Antibody used is specific to the nub
-PB and nub
Early in third instar, nub
is expressed in a broad region from the pretarsus through the presumptive tarsal segments, up to the distal part of the dac
domain in presumptive tarsal segment 2. By late third instar, nub
expression disappears from the intersegmental region of true segments and the pretarsal region, remaining only in the joint-forming cells.
Between 4 and 12 hr APF, nub
expression is proximally adjacent to Ecol\lacZodd-rK111
, which labels the proximal parts of all leg joints except those between tarsal subsegments.
protein is localized to proximal half of the true leg segments of leg discs; localization is proximal to that of bowl
protein is expressed in the wing pouch and distal wing hinge, but not in the presumptive notum. Expression is mutually exclusive with that of tsh
Expression first seen in the wing disc during middle to late second instar in an area that corresponds to the distal region of the wing. The nub
domain is a bit broader than the rn
Markers that uniquely identify the cells of the NB3-7 lineage were used to examine the serotonin expressing cell lineages.
Expression in procephalic neuroblasts stage 9-11: tritocerebrum - d1-6, v1, v5; deuterocerebrum - d1-8, v2-8; protocerebrum - ad1, ad2, ad4-9, ad12, ad15, ad17, av1, cd1-6, cd8-14, cd17, cd20, cd21, cv1, cv3-9, pd2, pd4, pd5, pd8, pd9, pd12, pd16, pv1-3
Protein is detected in ring pattern in proximal joint of third instar larval leg discs. At 34-38 hours after pupal formation protein is expressed in the tibial and not the tarsal leg joints in the proximal joint tissue and a subset of bristle lineage.
protein is expressed in the wing pouch region of the third instar wing disc.
is expressed in the lateral, but not the medial, of the paired ventro-lateral serotonergic neurons.
is expressed in early stages of the developing CNS in a highly dynamic pattern that includes roughly half of the neuroblasts and several ganglion mother cells. Here, nub
are shown to be coexpressed in GMC4-2a but not in its progenitor neuroblast or its mature progeny.