Gene: Dmel\pan
dTCF, TCF, l(4)13, LEF-1, T cell factor
transcription factor - HMG domain - segment polarity - acts to transduce the Wingless signal - toggles between acting as a transcriptional repressor (when bound to Groucho) and activator (when bound to Armadillo) to promote cell fate specification
Please see the JBrowse view of Dmel\pan for information on other features
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Gene model reviewed during 5.41
Gene model reviewed during 5.39
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.47
Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
2.8 (compiled cDNA)
751 (aa)
A fusion construct containing amino acids 1 to 130 of wild-type pan protein (panwt.cBa) binds 4-5 fold more of the vertebrate arm protein homolog beta-catenin in-vitro than fusion constructs containing amino acids 1 to 130 of either panS25 or panS28 protein. The panS25 and panS28 proteins each differ from wild type pan protein by one amino acid.
Repeats 3-8 of the arm protein interact with aa 1-90 of pan in a yeast two-hybrid assay. The DNA binding site of the pan HMG box was assayed, and revealed the consensus CCTTTGATCTT. Cotransfection of arm and pan vectors resulted in transcriptional activation of a CAT or a Ppyr\LUC reporter, and the C-terminus of arm protein was necessary for this activation. The transactivation capacity of the C-terminus of arm was further demonstrated by fusing it to a GAL4-DNA binding domain, and assaying expression of a CAT reporter.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\pan using the Feature Mapper tool.
Comment: reference states 0-2 hr AEL
GBrowse - Visual display of RNA-Seq signals
View Dmel\pan in GBrowse 2
Maps close to ci.
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
polyclonal
Source for merge of: pan IA5
Source for merge of: pan CG32005
Annotations CG17964 and CG32005 merged as CG34403 in release 5.2 of the genome annotation.
DNA-protein interactions: genome-wide binding profile assayed for pan protein in stage 9-11 embryos; ArrayExpress accession number E-MTAB-1184.
dsRNA made from templates generated with primers directed against this gene has been transfected into Kc cells.
ChEST reveals this is a target of Mef2.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
New annotation (CG32005) in release 3 of the genome annotation.
The pan gene product can function as either an activator or a repressor of wg-responsive genes depending on the state of the wg signalling pathway and thus the availability of arm, the pan product coactivator. In the absence of arm, pan acts to repress wg responsive genes, with the gro protein acting as a corepressor.
Genomic organisation of pan is determined: a single copy gene spanning approximately 50kb and composed of 13 exons that share conservation with the exon/intron boundaries of human TCF-1.
Mutations in pan cause a segment polarity phenotype.
The gene is named 'pangolin' due to the 'lawn of denticles' phenotype of pan13 homozygotes.
