FlyBase Gene Report: Dmel\CG34435

General Information

Symbol

Dmel\CG34435

Species

D. melanogaster

Name

Annotation Symbol

CG34435

Feature Type

protein_coding_gene

FlyBase ID

FBgn0085464

Gene Model Status

Current

Stock Availability

1 publicly available

Gene Snapshot

Contributions welcome

Other Summaries

Alliance
Auto summary
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

Sequence location

X:5,613,325..5,614,467 [-]

Recombination map

1-12

RefSeq locus

NC_004354 REGION:5613325..5614467

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (2 terms)

Molecular Function (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in multicellular organism reproduction

inferred from high throughput expression pattern

(Findlay et al., 2008)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in extracellular space

inferred from high throughput direct assay

(Findlay et al., 2008)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

EF-hand domain pair

Summaries

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\CG34435 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.51

Sequence Ontology: Class of Gene

nuclear_gene

(FlyBase Genome Annotators, 2007)

protein_coding_gene

(FlyBase Genome Annotators, 2007)

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

CG34435-RA

FBtr0112736

NM_132005

1084

299

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

CG34435-PA

FBpp0111648

34.2

299

4.78

NP_572233

AAF46045

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

EF-hand domain pair

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9W4B1

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CG34435 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG34435_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in extracellular space

inferred from high throughput direct assay

(Findlay et al., 2008)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\CG34435 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CG34435

View exonic expression by tissue for Dmel\CG34435

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0085464

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG15780-RA

Flygut - An atlas of the Drosophila adult midgut

FBgn0085464

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 0 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 1 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CG34435

Transgenic constructs containing regulatory region of CG34435

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation

Inferred from location

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (5)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\MYL6B

3 of 15

Yes

Hsap\MYL6

3 of 15

Yes

Hsap\MYL1

2 of 15

Hsap\MYL3

2 of 15

Hsap\MYL4

2 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (5)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Myl6b

3 of 15

Yes

Mmus\Myl6

3 of 15

Yes

Mmus\Myl1

2 of 15

Mmus\Myl3

2 of 15

Mmus\Myl4

2 of 15

Rattus norvegicus (Norway rat) (6)

Rnor\Myl1

2 of 13

Yes

Rnor\Myl3

2 of 13

Yes

Rnor\Myl4

2 of 13

Yes

Rnor\Myl6l

1 of 13

Rnor\Myl6

1 of 13

Rnor\RGD1560334

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (4)

Xtro\myl1

2 of 12

Yes

Xtro\myl3

2 of 12

Yes

Xtro\myl4

2 of 12

Yes

Xtro\myl6

2 of 12

Yes

Danio rerio (Zebrafish) (8)

Drer\cmlc1

2 of 15

Yes

Drer\myl1

2 of 15

Yes

Drer\myl4

2 of 15

Yes

Drer\myl6

2 of 15

Yes

Drer\myl13

2 of 15

Yes

Drer\mylz3

2 of 15

Yes

Drer\zgc:153867

2 of 15

Yes

Drer\zgc:163073

2 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (4)

Cele\mlc-6

3 of 15

Yes

Cele\mlc-7

2 of 15

Cele\cal-7

1 of 15

Cele\mlc-5

1 of 15

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\MLC1

2 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\cdc4

2 of 12

Yes

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190D87 )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA13953

Drosophila persimilis

Dper\GL16473

Drosophila willistoni

Dwil\GK10180

Drosophila virilis

Dvir\GJ16950

Drosophila mojavensis

Dmoj\GI21778

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( None identified )

No non-Drosophilid orthologies identified

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( None identified )

No non-Dipteran orthologies identified

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( None identified )

No non-Insect Arthropod orthologies identified

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )

No non-Arthropod Metazoa orthologies identified

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (4)

Gene Symbol

Score

Source

Alignment

2 of 10

2 of 10

2 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

MYL6B; myosin light chain 6B

3 of 15

609930

MYL6; myosin light chain 6

3 of 15

609931

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

CG34435

two hybrid

(Hu et al., 2017.6.13)

CG34435 - Gsc

two hybrid

(Hu et al., 2017.6.13)

CG34435 - Vta1

two hybrid

(Hu et al., 2017.6.13)

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0085464

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-12

Cytogenetic map

5A7-5A8

Sequence location

X:5,613,325..5,614,467 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

5A7-5A8

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-12

(FlyBase, 2016)

1-14.4

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (1)

Bloomington

57401

y¹ sc^* v¹ sev²¹; P{TRiP.HMC04705}attP40

Genomic Clones (23)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (2)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

MIP10861

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0085464

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

DMG2-chrX.6.034.a

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0085464

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

31471

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Additional comments

Annotation CG15780 has been split in release 5.2 of the genome annotation. Sequences from the 3' half of release 5.1 annotation CG15780 are present in release 5.2 annotation CG34435. Sequences from the 5' half of release 5.1 annotation CG15780 have been merged with sequences from release 5.1 annotation CG17763 to form release 5.2 annotation CG34434 (although the reading frame of this new annotation is different from much of that present in release 5.1 annotation CG15780).

(FlyBase Genome Annotators, 2007)

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 18 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

31471

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.