FlyBase Gene Report: Dmel\Myo31DF

General Information

Symbol

Dmel\Myo31DF

Species

D. melanogaster

Name

Myosin 31DF

Annotation Symbol

CG7438

Feature Type

protein_coding_gene

FlyBase ID

FBgn0086347

Gene Model Status

Current

Stock Availability

42 publicly available

Enzyme Name (EC)

Myosin ATPase (5.6.1.8)

Gene Summary

Unconventional myosin that functions as actin-based motor protein with ATPase activity (PubMed:30467170). Binds to membranes enriched in phosphatidylinositol 4-5-bisphosphate, and can glide along actin filaments when anchored to a lipid bilayer (PubMed:30467170). Generates left-right asymmetry at the level of single cells, organs and the whole body via its interaction with the actin cytoskeleton, both in the embryo and the adult (PubMed:16598258, PubMed:16598259, PubMed:18521948, PubMed:26073018, PubMed:25659376, PubMed:30467170). Normal left-right asymmetry of the larval midgut and hindgut requires expression in the embryonic hindgut epithelium during a critical time period, 10 to 12.75 hours after egg laying (PubMed:18521948). This period corresponds to a late stage of germband retraction, and precedes left-right asymmetric morphogenesis (PubMed:18521948). Expression in segment H1 of the imaginal ring is required at 0 to 24 hours after pupation for changes of cell shape and orientation in the H2 segment, which then gives rise to normal, dextral looping of the adult hindgut (PubMed:16598258, PubMed:26073018). Required during a critical period, 126-132 hours after egg laying, for normal, dextral rotation of the adult male genitalia (PubMed:16598258, PubMed:16598259, PubMed:22491943, PubMed:26073018, PubMed:25659376). Has a double role by promoting dextral rotation in the posterior compartment of segment A8 of the male genital disk, and in repressing sinistral looping in the anterior compartment (PubMed:16598259). (UniProt, Q23978)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

Myo1A, Myo1D, Myosin IA, MyoIA, myoID

Key Links

Genomic Location

Cytogenetic map

31F1-31F4

Sequence location

2L:10,491,814..10,506,779 [-]

Recombination map

2-43

RefSeq locus

NT_033779 REGION:10491814..10506779

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

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Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (18 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables microfilament motor activity

inferred from direct assay

(assigned by UniProt )

(Lebreton et al., 2018)

inferred from mutant phenotype

(assigned by UniProt )

(Lebreton et al., 2018)

enables phosphatidylinositol-4,5-bisphosphate binding

inferred from direct assay

(assigned by UniProt )

(Lebreton et al., 2018)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables actin filament binding

inferred from biological aspect of ancestor with PANTHER:PTN000320364

(assigned by GO_Central )

(Gaudet et al., 2011)

enables ATP binding

inferred from electronic annotation with InterPro:IPR001609

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables microfilament motor activity

inferred from biological aspect of ancestor with PANTHER:PTN000320364

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (7 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

involved_in determination of digestive tract left/right asymmetry

inferred from mutant phenotype

(Okumura et al., 2015, Taniguchi et al., 2011)

involved_in determination of left/right symmetry

inferred from mutant phenotype

(Ishibashi et al., 2019, Okumura et al., 2015, Petzoldt et al., 2012, Suzanne et al., 2010, Taniguchi et al., 2007, Hozumi et al., 2006, Speder et al., 2006)

inferred from mutant phenotype

(assigned by UniProt )

(Lebreton et al., 2018)

involved_in hindgut development

inferred from mutant phenotype

(Ishibashi et al., 2019)

involved_in imaginal disc-derived male genitalia morphogenesis

inferred from mutant phenotype

(Petzoldt et al., 2012, Suzanne et al., 2010)

involved_in mesoderm development

inferred from expression pattern

(Furlong et al., 2001)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in actin filament organization

inferred from biological aspect of ancestor with PANTHER:PTN000320364

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in vesicle transport along actin filament

inferred from biological aspect of ancestor with PANTHER:PTN000320364

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (7 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in cell cortex

inferred from direct assay

(Petzoldt et al., 2012)

Terms Based on Predictions or Assertions (6 terms)

CV Term

Evidence

References

is_active_in actin cytoskeleton

inferred from biological aspect of ancestor with PANTHER:PTN000320364

(assigned by GO_Central )

(Gaudet et al., 2011)

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000320364

(assigned by GO_Central )

(Gaudet et al., 2011)

is_active_in microvillus

inferred from biological aspect of ancestor with PANTHER:PTN001406997

(assigned by GO_Central )

(Gaudet et al., 2011)

part_of myosin complex

inferred from electronic annotation with InterPro:IPR001609, InterPro:IPR010926

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN001406997

(assigned by GO_Central )

(Gaudet et al., 2011)

is_active_in vesicle

inferred from biological aspect of ancestor with PANTHER:PTN000320364

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

MYOSIN MOTORS

Protein Family (UniProt)

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family. (Q23978)

Protein Signatures (InterPro)

Catalytic Activity (EC)

Experimental Evidence

ATP + H(2)O + myosin bound to actin filament at position(n) = ADP + phosphate + myosin bound to actin filament at position(n+1) (5.6.1.8)

Predictions / Assertions

ATP + H(2)O + myosin bound to actin filament at position(n) = ADP + phosphate + myosin bound to actin filament at position(n+1) (5.6.1.8)

Summaries

Gene Group (FlyBase)

MYOSIN MOTORS -: Myosin motors are motor proteins that use the hydrolysis of ATP to drive movement along actin filaments. They can be divided into two groups: conventional myosins (myosin II) are involved in generating the mechanical force for muscle contraction; unconventional myosins (all other classes) are involved in processes such as cell motility, cytokinesis and trafficking. (Adapted from FBrf0134714.)

Protein Function (UniProtKB)

(UniProt, Q23978)

Summary (Interactive Fly)

cytoskeletal motor protein required in the hindgut epithelium and testes for normal embryonic handedness (left-right asymmetry)

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Myo31DF for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q23978)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.48

Sequence Ontology: Class of Gene

gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Myo31DF-RA

FBtr0080035

NM_078814

3741

1011

Myo31DF-RB

FBtr0080036

NM_164933

4318

1011

Myo31DF-RC

FBtr0304138

NM_001201855

3882

1011

Additional Transcript Data and Comments

Reported size (kB)

4.4, 3.8 (northern blot)

(Morgan et al., 1994)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Myo31DF-PA

FBpp0079625

117.1

1011

9.89

NP_523538

AAF52966

Myo31DF-PB

FBpp0079626

117.1

1011

9.89

NP_723596

AAN10756

Myo31DF-PC

FBpp0293078

117.1

1011

9.89

NP_001188784

ADV37034

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1011 aa isoforms: Myo31DF-PA, Myo31DF-PB, Myo31DF-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

1011 (aa); 117 (kD predicted)

(Morgan et al., 1994)

Comments

External Data

Subunit Structure (UniProtKB)

Binds to F-actin (PubMed:7589814). Interacts with arm (PubMed:16598259, PubMed:22491943). Interacts with shg (PubMed:22491943). Interacts with ds (via intracellular region) (PubMed:26073018).

(UniProt, Q23978)

Domain

The myosin motor domain contains the derminants for dextral twisting.

The two IQ domains are essential for activity in determining left-right asymmetry.

The actin-binding domain is essential for activity in determining left-right asymmetry.

(UniProt, Q23978)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q23978

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Myo31DF using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Myo31DF_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

hindgut anlage in statu nascendi

(Fisher et al., 2012)

posterior ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

embryonic stage 7 -- 8

hindgut anlage

(Fisher et al., 2012)

embryonic stage 9 -- 10

hindgut proper primordium

Comment: reported as hindgut proper anlage

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

ventral ectoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

dorsal epidermis primordium

(Fisher et al., 2012)

foregut primordium

(Fisher et al., 2012)

hindgut proper primordium

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

salivary gland body primordium

(Fisher et al., 2012)

visceral muscle primordium

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Morgan et al., 1994)

single cell RNA-seq

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult midgut enterocyte

(Xu et al., 2019)

Additional Descriptive Data

Myo31DF transcripts are detected throughout development on northern blots. The 3.8kb transcript is less abundant than the 4.4kb transcript.

(Morgan et al., 1994)

Marker for

adult midgut enterocyte

(Xu et al., 2019)

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

late embryonic stage

embryonic/larval proventriculus

(Morgan et al., 1995)

gastric caecum

(Morgan et al., 1995)

embryonic/larval hindgut

(Morgan et al., 1995)

embryonic/larval digestive system

(Morgan et al., 1995)

embryonic/larval midgut

(Morgan et al., 1995)

early third instar larval stage -- prepupal stage P4

abdominal segment 8 of male genital disc

(Speder et al., 2006)

prepupal stage P4(i) | male

abdominal segment 8 of male genital disc

(Petzoldt et al., 2012)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Morgan et al., 1995)

Additional Descriptive Data

At embryonic stage 16, Myo31DF protein is concentrated at the basolateral domain of immature enterocytes and is diffusely located in the cytoplasm. By stage 17, slightly more apical staining is observed in most regions of the gut. In larvae, Myo31DF protein is enriched in the brush border in the subapical terminal web domain. In adults, it is found in the microvilli in addition to its terminal web locale.

(Petzoldt et al., 2012, Morgan et al., 1995)

Myo31DF protein is expressed in a symmetrical double chevron pattern restricted to the ventral domain of the male genital disc; expression is restricted to the A8 segment of the disc. Expression begins early in the third instar, and maintains a consistent pattern through prepupal stage P4.

(Speder et al., 2006)

Myo31DF protein is first detected on western blots in 4-8hr embryos, is present in appreciable amounts in 8-12hr embryos, peaks in third instar larvae, decreases dramatically during pupation, and is present at low levels in adults. Myo31DF protein is detected by immunolocalization in the alimentary canal and its derivatives and weakly in egg chambers. In late embryos, it is uniformly distributed throughout the midgut and hindgut but not in the foregut. Some staining is observed in the proventriculus and in the gastric caeca.

(Morgan et al., 1995)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cell cortex

inferred from direct assay

(Petzoldt et al., 2012)

Expression Deduced from Reporters

Reporter: P{GawB}Myo31DF^NP0001

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

midgut imaginal island

(Jiang and Edgar, 2009)

enterocyte

(Jiang and Edgar, 2009)

adult stage

adult gut

(Zaidman-Remy et al., 2006)

adult midgut

(Buchon et al., 2009)

enterocyte

(Jiang et al., 2011, Buchon et al., 2010)

Reporter: P{GawB}Myo31DF^NP1242

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

projection neuron | subset of adult medial antennal lobe tract

(Masuda-Nakagawa et al., 2010)

embryonic/larval antennal lobe glomerulus | restricted | subset

(Masuda-Nakagawa et al., 2010)

Kenyon cell | subset

(Masuda-Nakagawa et al., 2010)

larval MB calyx glomerulus A2 | restricted

(Masuda-Nakagawa et al., 2010)

Reporter: P{GawB}Myo31DF^NP1548

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

larval hindgut imaginal ring | anterior

(González-Morales et al., 2015)

early third instar larval stage -- prepupal stage P4

abdominal segment 8 of male genital disc

(Speder et al., 2006)

pupal stage

genital disc | restricted

(Suzanne et al., 2010)

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Myo31DF in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Myo31DF

View exonic expression by tissue for Dmel\Myo31DF

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0086347

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34445/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0086347

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0086347

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0086347

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7438

Flygut - An atlas of the Drosophila adult midgut

FBgn0086347

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Myo31DF

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 31 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 20 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Myo31DF

Transgenic constructs containing regulatory region of Myo31DF

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(2L)ED8142

(Bosch and Perrimon, 2019.9.30)

Df(2L)Exel7048

(Ryder, 2004.4.26)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

partially lethal - majority die, with Scer\GAL4^pnr-MD237

Myo31DF^GD4046

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Myo31DF^GD4046

viable, with Scer\GAL4^tin.CΔ4

Myo31DF^GD4046

Sterility

Allele

fertile

Other Phenotypes

Allele

abnormal axis specification

Myo31DF^L152

abnormal axis specification | embryonic stage, with Scer\GAL4^da.G32

Myo31DF^UAS.Tag:HA

abnormal cell shape | embryonic stage

Myo31DF^L152

flightless, with Scer\GAL4^Mef2.PR

Myo31DF^GD4046

long lived, with Scer\GAL4^hs.PB

Myo31DF^GS2116

some die during pupal stage, with Scer\GAL4^pnr-MD237

Myo31DF^GD4046

visible, with Scer\GAL4^pnr-MD237

Myo31DF^GD4046

Phenotype manifest in

Allele

adult hindgut

adult hindgut, with Scer\GAL4^byn-Gal4

Myo31DF^{dsRNA.Sym.UAS}

embryonic/larval hindgut

Myo31DF^L152

embryonic/larval midgut primordium

embryonic/larval midgut primordium (with Df(2L)J2)

Myo31DF^L152

embryonic/larval posterior midgut

Myo31DF^L152

embryonic foregut, with Scer\GAL4^da.G32

embryonic hindgut

embryonic hindgut (with Df(2L)J2)

Myo31DF^L152

male genitalia

male genitalia, with Scer\GAL4^Abd-B-LDN

Myo31DF^{dsRNA.Sym.UAS}

male genitalia, with Scer\GAL4^dpp.blk1

Myo31DF^{dsRNA.Sym.UAS}

male genitalia, with Scer\GAL4^en-e16E

Myo31DF^{dsRNA.Sym.UAS}

male genitalia, with Scer\GAL4^hh-Gal4

Myo31DF^{dsRNA.Sym.UAS}

male genitalia, with Scer\GAL4^ptc-559.1

Myo31DF^{dsRNA.Sym.UAS}

male genitalia, with Scer\GAL4^tsh-md621

Myo31DF^{dsRNA.Sym.UAS}

male genitalia (with Df(2L)Exel7048)

Myo31DF^KG02246

male genitalia (with Df(2L)J3)

Myo31DF^KG02246

male genitalia | heat sensitive, with Scer\GAL4^tsh-md621, Scer\GAL80^ts.αTub84B

Myo31DF^{dsRNA.Sym.UAS}

testis

trichogen cell, with Scer\GAL4^pnr-MD237

Myo31DF^GD4046

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (9)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\MYO1D

14 of 15

Yes

Hsap\MYO1G

12 of 15

Yes

4 of 15

4 of 15

4 of 15

4 of 15

Yes

Hsap\MYO1F

4 of 15

Yes

Hsap\MYO1H

3 of 15

Hsap\MYO6

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (9)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Myo1d

14 of 15

Yes

Mmus\Myo1g

12 of 15

Yes

Mmus\Myo1a

4 of 15

Mmus\Myo1c

4 of 15

Mmus\Myo1e

4 of 15

Yes

Mmus\Myo1f

4 of 15

Yes

Mmus\Myo1b

3 of 15

Mmus\Myo1h

3 of 15

Mmus\Myo6

1 of 15

Rattus norvegicus (Norway rat) (10)

Rnor\Myo1d

12 of 13

Yes

Rnor\Myo1g

7 of 13

Yes

Rnor\Myo1c

4 of 13

Rnor\Myo1a

3 of 13

Rnor\Myo1f

3 of 13

Yes

Rnor\Myo1b

2 of 13

Rnor\Myo1e

2 of 13

Yes

Rnor\LOC108352465

1 of 13

Yes

Rnor\Myo1h

1 of 13

Yes

Rnor\Myo6

1 of 13

Xenopus tropicalis (Western clawed frog) (8)

Xtro\myo1d

12 of 12

Yes

3 of 12

3 of 12

3 of 12

2 of 12

Yes

Xtro\myo1f

2 of 12

Yes

Xtro\myo1e.1

1 of 12

Yes

Xtro\myo6

1 of 12

Danio rerio (Zebrafish) (12)

Drer\myo1d

12 of 15

Yes

Drer\myo1g

12 of 15

Yes

4 of 15

4 of 15

4 of 15

4 of 15

Yes

Drer\myo1f

4 of 15

Yes

Drer\myo1ha

4 of 15

Drer\myo1hb

4 of 15

Drer\myo1eb

3 of 15

Yes

Drer\myo6a

1 of 15

Drer\myo6b

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (6)

Cele\hum-5

14 of 15

Yes

Cele\hum-1

4 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

Arabidopsis thaliana (thale-cress) (4)

Atha\ATM1

2 of 9

Yes

Atha\ATM2

2 of 9

Yes

Atha\VIIIB

2 of 9

Yes

Atha\VIIIA

1 of 9

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\MYO3

5 of 15

Yes

Scer\MYO5

5 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\myo1

5 of 12

Yes

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (14)

Gene Symbol

Score

Source

Alignment

6 of 10

5 of 10

jar

3 of 10

2 of 10

2 of 10

2 of 10

2 of 10

1 of 10

1 of 10

Mhc

1 of 10

Myo81F

1 of 10

Prm

1 of 10

zip

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

MYO1E; myosin IE

model of focal segmental glomerulosclerosis 6

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Myo31DF^KK102456

ameliorates carcinoma

modeled by Ras85D^V12.UAS, scrib⁶⁷³

(Amcheslavsky et al., 2018)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

14 of 15

12 of 15

4 of 15

4 of 15

4 of 15

4 of 15

FOCAL SEGMENTAL GLOMERULOSCLEROSIS 6; FSGS6

focal segmental glomerulosclerosis 6

4 of 15

3 of 15

CENTRAL HYPOVENTILATION SYNDROME, CONGENITAL, 2, AND AUTONOMIC DYSFUNCTION; CCHS2

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Myo31DF - DAAM

anti tag coimmunoprecipitation, anti tag western blot

(Chougule et al., 2020)

Myo31DF - Dronc

phenotype-based detection assay, fluorescence microscopy, inferred by author, anti tag coimmunoprecipitation, western blot

(Amcheslavsky et al., 2018)

Myo31DF - Mer

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kwon et al., 2013)

Myo31DF - Nrd1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Yoon et al., 2017)

Myo31DF - SMC5

anti tag coimmunoprecipitation, anti tag western blot

(Caridi et al., 2018)

Myo31DF - arm

pull down, autoradiography, anti tag coimmunoprecipitation, western blot, two hybrid

(Petzoldt et al., 2012, Speder et al., 2006)

Myo31DF - ds

anti tag coimmunoprecipitation, western blot, anti tag western blot

(González-Morales et al., 2015)

Myo31DF - jnj

anti tag coimmunoprecipitation, anti tag western blot

(Caridi et al., 2018)

Myo31DF - scra

anti tag coimmunoprecipitation, peptide massfingerprinting

(D'Avino et al., 2008)

Myo31DF - shg

anti tag coimmunoprecipitation, western blot

(Petzoldt et al., 2012)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Myo31DF

enhanceable

Myo61F

(Okumura et al., 2015)

Myo31DF

enhanceable

Myo61F|Myo95E

(Okumura et al., 2015)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Myo61F

suppressible

Myo31DF

(Petzoldt et al., 2012)

pbl

suppressible

Myo31DF

(Gregory et al., 2007)

pbl

enhanceable

Myo31DF

(Gregory et al., 2007)

External Data

Subunit Structure (UniProtKB)

Binds to F-actin (PubMed:7589814). Interacts with arm (PubMed:16598259, PubMed:22491943). Interacts with shg (PubMed:22491943). Interacts with ds (via intracellular region) (PubMed:26073018).

(UniProt, Q23978 )

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0086347

MIST (genetic) - An integrated Molecular Interaction Database

Myo31DF

MIST (protein-protein) - An integrated Molecular Interaction Database

Myo31DF

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-43

Cytogenetic map

31F1-31F4

Sequence location

2L:10,491,814..10,506,779 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

31F1-31F4

Limits computationally determined from genome sequence between P{lacW}KdelR^k00311 and P{lacW}l(2)k09116^k09116&P{lacW}Fatp^k10307

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

31D-31F

(Morgan, 1994.3.8)

31D-31F

(determined by in situ hybridisation)

(Morgan et al., 1994)

Experimentally Determined Recombination Data

Location

2-43

(FlyBase, 2016)

2-35.7

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (42)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

1521

w^*; P{UAS-GFP.S65T}Myo31DF^T2

6313

P{GawB}lz^gal4; P{UAS-GFP.S65T}Myo31DF^T2

84307

w^*; P{GawB}Myo31DF^NP0001; P{UAS-GC3Ai}3

84328

w^*; P{GawB}Myo31DF^NP0001; P{UAS-VC3Ai}3

84286

w^*; P{GawB}Myo31DF^NP0001; P{UAS-CC3Ai}3

19940

y¹ w^67c23; P{EPgy2}Myo31DF^EY08859

6795

w^*; P{nrv2-GAL4.S}3 P{UAS-GFP.S65T}Myo31DF^T2

Kyoto

106363

w^*; P{UAS-GFP.S65T}Myo31DF^T2

125255

w¹¹¹⁸; P{RS5}Myo31DF^5-HA-1552

115611

w¹¹¹⁸; PBac{806.LOX-SVS-2}Myo31DF^CPTI004107

VDRC

v104089

P{KK102456}VIE-260B

v12558

w¹¹¹⁸; P{GD4046}v12558

More stocks available...

Genomic Clones (32)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (97)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

SD01662

Other clones

DPiM_SD01662

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0086347

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0086347

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

34445

Antibody Information

Laboratory Generated Antibodies

monoclonal

(Amcheslavsky et al., 2018)

polyclonal

(Hatori et al., 2014, Petzoldt et al., 2012)

monoclonal, polyclonal

(Morgan et al., 1995)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: Myo31DF souther

(Hozumi et al., 2006)

Additional comments

Other Comments

Myo31DF mutants show reversed laterality of the embryonic and adult gut and testis.

(Taniguchi et al., 2007)

Myo31DF is required for normal left-right handedness of the embryonic midgut and hindgut.

(Hozumi et al., 2006)

Myo31DF is required for the normal dextral looping of the male genitalia that occurs during development.

(Speder et al., 2006)

Indentification: In a genetic screen for mutations that show situs inversus or randomization of the gut left-right asymmetry.

(Hozumi et al., 2003)

In souther mutant embryos, left-right inversion in the shape of the midgut and hindgut was observed at approximately 50%.

(Hozumi et al., 2003)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation.

(Rogers et al., 2003)

The products of the Myo31DF and Myo61F loci are associated with the brush border cytoskeleton.

(Morgan et al., 1995)

Isolation and characterisation of Myo31DF.

(Morgan et al., 1994)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 55 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34445

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q23978

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q23978

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q23978

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0086347

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0086347

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34445/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0086347

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Myo31DF_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0086347

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0086347

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7438

Flygut - An atlas of the Drosophila adult midgut

FBgn0086347

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

34445

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC005895

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG7438

MARRVEL_MODEL - MARRVEL (model organism gene)

34445

modMine - A data warehouse for the modENCODE project

FBgn0086347

Linkouts

ApoDroso - Functional genomic database for photoreceptor development, survival and function

10863-Myosin31DF[k09116]

DroID - A comprehensive database of gene and protein interactions.

FBgn0086347

DRSC - Results frm RNAi screens

FBgn0086347

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0086347

FlyMine - An integrated database for Drosophila genomics

FBgn0086347

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (genetic) - An integrated Molecular Interaction Database

Myo31DF

MIST (protein-protein) - An integrated Molecular Interaction Database

Myo31DF

Synonyms and Secondary IDs (33)

Reported As

Symbol Synonym

CG7438

(Kolkhof et al., 2017, Hosono et al., 2015, Rosa-Ferreira et al., 2015, Haye et al., 2014, Christis and Munro, 2012, Hadar et al., 2012, Petzoldt et al., 2012, Cammarato et al., 2011, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Yi et al., 2007, Speder et al., 2006, Rogers et al., 2003, Seong et al., 2001, Goldstein and Gunawardena, 2000)

Dm IA

(Hodge and Cope, 2000)

DmIA

(Yamashita et al., 2000)

DroMIA

(Morgan, 1995, Morgan et al., 1994, Morgan et al., 1993)

(Titus, 1997)

MYOIA

(Goldstein and Gunawardena, 2000)

Mhc1

(Lindsey et al., 2021, Karageorgiou et al., 2020, Kollmar, 2015.9.15)

Myo-IA

(Berg et al., 2001)

Myo1A

(Caridi et al., 2018, Driver and Ohlstein, 2014, Zhou et al., 2013, Lee et al., 2012, Poernbacher et al., 2012, Jiang et al., 2011, Apidianakis et al., 2009, Oliver et al., 1999)

Myo1D

(Hounsell and Fan, 2021, Amcheslavsky et al., 2020, Chougule et al., 2020, Amcheslavsky et al., 2018, Lebreton et al., 2018, Packard et al., 2015)

Myo1a

(Bosch et al., 2020)

Myo31DF

(Takakura et al., 2021, Axelrod, 2020, Hamada and Tam, 2020, Bosch and Perrimon, 2019.9.30, Inatomi et al., 2019, Yildirim et al., 2019, Gene Disruption Project members, 2018-, Inaki et al., 2018, Aw et al., 2017, Fochler et al., 2017, Nie et al., 2017, Yoon et al., 2017, Inaki et al., 2016, Kuleesha et al., 2016, Dent et al., 2015, Okumura et al., 2015, Packard et al., 2015, Ashwal-Fluss et al., 2014, Hatori et al., 2014, Kwon et al., 2013, Kwon et al., 2013, Wilson et al., 2013, Zhu and Zhang, 2013, Hadar et al., 2012, Izutsu et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Takahashi et al., 2012, Chatterjee et al., 2011, Taniguchi et al., 2011, Taniguchi et al., 2011, Kazemian et al., 2010, Kong et al., 2010, Skora and Spradling, 2010, Ayroles et al., 2009, Hozumi et al., 2008, Inomata et al., 2008, Buszczak et al., 2007, Georlette et al., 2007, Gregory et al., 2007, Spéder et al., 2007, Stuart et al., 2007, Taniguchi et al., 2007, Yi et al., 2007, Zhang et al., 2007, Baum, 2006, Hozumi et al., 2006, Speder et al., 2006, Cryderman et al., 2005, Kim et al., 2005, Raymond et al., 2004)

MyoIA

(Lan et al., 2018, Tang et al., 2018, Li et al., 2013, Jiang and Edgar, 2009, Jiang et al., 2009)

MyoID

(Cohen et al., 2020, Ishibashi et al., 2019, Petzoldt et al., 2012, Taniguchi et al., 2011)

mhc1

(Palavalli et al., 2021)

myo1D

(Chougule et al., 2020, Yoong et al., 2020)

myo31DF

(Sato et al., 2015, Haye et al., 2014)

myoID

(González-Morales et al., 2015, Sato et al., 2015, Bulgakova et al., 2012, Suzanne et al., 2010)

souther

Name Synonyms

Myo31DF

(Cammarato et al., 2011)

Myosin 1D

(Hozumi et al., 2006)

Myosin 31DF

(Chatterjee et al., 2011, D'Avino et al., 2008, Gregory et al., 2007)

Myosin IA

(Reed et al., 2012, Serio et al., 2010, Jiang and Edgar, 2009, Jiang et al., 2009, Rogers et al., 2003)

Myosin ID

(Ishibashi et al., 2019, Taniguchi et al., 2011)

Myosin-IA

Myosin31DF

(Inaki et al., 2018, Hozumi et al., 2008, Taniguchi et al., 2007)

myosin 1a

(Wolenski, 1995)

myosin I

(Miller et al., 1991)

myosin IA

(Rogers et al., 2003, Goldstein and Gunawardena, 2000)

myosin ID

(Suzanne et al., 2010)

myosin-IA

(Morgan, 1995, Morgan et al., 1995, Morgan, 1994.3.8, Morgan et al., 1994)

myosin1A

(Apidianakis et al., 2009)

souther

(Hozumi et al., 2003)

type ID unconventional myosin 31DF

(Sato et al., 2015)

unconventional myosin 31DF

(Spéder et al., 2007)

Secondary FlyBase IDs

FBgn0011673
FBgn0064139

Datasets (8)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (8)

Result

Result Type

Title

scRNAseq_2020_Cattenoz_NI_seq_clustering

cell clustering analysis

Clustering analysis of hemocytes from non-infested third instar larvae

scRNAseq_2020_Cattenoz_WI_seq_clustering

cell clustering analysis

Clustering analysis of hemocytes from wasp-infested third instar larvae

scRNAseq_2020_Cho_NI72LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 72 h after egg-laying

scRNAseq_2020_Cho_NI96LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI120LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 120 h after egg-laying

scRNAseq_2020_Cho_WI96LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from wasp-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI96HL_seq_clustering

cell clustering analysis

Clustering analysis of circulating hemocytes from non-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI120HL_seq_clustering

cell clustering analysis

Clustering analysis of circulating hemocytes from non-infested larvae at 120 h after egg-laying

References (161)

The following external sites may use different assemblies or annotations than FlyBase.

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