Myo1A, MyoIA, Myosin IA, myoID, myosin-IA
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Gene model reviewed during 5.48
4.4, 3.8 (northern blot)
1011 (aa); 117 (kD predicted)
The myosin motor domain contains the derminants for dextral twisting.
The two IQ domains are essential for activity in determining left-right asymmetry.
The actin-binding domain is essential for activity in determining left-right asymmetry.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Myo31DF using the Feature Mapper tool.
At embryonic stage 16, Myo31DF protein is concentrated at the basolateral domain of immature enterocytes and is diffusely located in the cytoplasm. By stage 17, slightly more apical staining is observed in most regions of the gut. In larvae, Myo31DF protein is enriched in the brush border in the subapical terminal web domain. In adults, it is found in the microvilli in addition to its terminal web locale.
Myo31DF protein is expressed in a symmetrical double chevron pattern restricted to the ventral domain of the male genital disc; expression is restricted to the A8 segment of the disc. Expression begins early in the third instar, and maintains a consistent pattern through prepupal stage P4.
Myo31DF protein is first detected on western blots in 4-8hr embryos, is present in appreciable amounts in 8-12hr embryos, peaks in third instar larvae, decreases dramatically during pupation, and is present at low levels in adults. Myo31DF protein is detected by immunolocalization in the alimentary canal and its derivatives and weakly in egg chambers. In late embryos, it is uniformly distributed throughout the midgut and hindgut but not in the foregut. Some staining is observed in the proventriculus and in the gastric caeca.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Myo31DF in GBrowse 2
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Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for merge of: Myo31DF souther
Myo31DF mutants show reversed laterality of the embryonic and adult gut and testis.
Myo31DF is required for normal left-right handedness of the embryonic midgut and hindgut.
Myo31DF is required for the normal dextral looping of the male genitalia that occurs during development.
Indentification: In a genetic screen for mutations that show situs inversus or randomization of the gut left-right asymmetry.
In souther mutant embryos, left-right inversion in the shape of the midgut and hindgut was observed at approximately 50%.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation.