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Gene Dmel\Tpi

General Information
Symbol	Dmel\Tpi	Species	D. melanogaster
Name	Triose phosphate isomerase	Annotation symbol	CG2171
Feature type	protein_coding_gene	FlyBase ID	FBgn0086355
Gene Model Status	Current	Stock availability	10 publicly available
Genomic Location
Chromosome (arm)	3R	Recombination map	3-101.3
Cytogenetic map	99D8-99D8	Sequence location	3R:25,958,703..25,960,321 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Triose phosphate isomerase is referred to in FlyBase by the symbol Dmel\Tpi (CG2171, FBgn0086355). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: triose-phosphate isomerase activity. There is experimental evidence that it is involved in the biological process: response to mechanical stimulus; determination of adult lifespan; neurological system process. 20 alleles are reported. The phenotypes of these alleles are annotated with: thoracico-abdominal ganglion; central nervous system; adult brain. It has 3 annotated transcripts and 3 annotated polypeptides. Protein features are: Aldolase-type TIM barrel; Triosephosphate isomerase; Triosephosphate isomerase, active site; Triosephosphate isomerase, bacterial/eukaryotic. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed within 00-06 and 12-24 hour embryonic stages, at stages throughout the larval period, during late pupal stages, in stages of adults of both sexes. Summary of FlyAtlas Anatomical Expression Data: High levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1637816_s_at completely aligns to an exonic region common to each of the 3 FlyBase-annotated transcript isoforms of Tpi. Gene sequence location is 3R:25958703..25960321.
User Contributed Data
	Beta version, feedback welcome
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	Tpi: Triosephosphate isomerase Structural gene for the glycolytic enzyme triosphosphate isomerase [TPI (EC5.3.1.1)] found in muscle. Heterozygote contains the parental isozymes plus a hybrid isozyme.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 99D8-99D8   Limits computationally determined from genome sequence between P{PZ}ncd05884 and P{lacW}l(3)s2500s2500
Experimentally Determined Cytological Location
Cytogenetic map Notes References 99E-99E   (determined by in situ hybridisation)   (Shaw-Lee et al., 1991)
Experimentally Determined Recombination Data
Location	3-101.3   3-100 +/- 3 (Palladino et al., 2002)
Left of (cM)	bv
Right of (cM)	Acph-1
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Tpi for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.47
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Tpi-RA FBtr0085582  NM_176587   1374   348 Tpi-RB FBtr0085583  NM_176588   1112   247 Tpi-RC FBtr0085584  NM_176589   1268   247
Additional Transcript Data & Comments
Reported size (kB)	1.3 (northern blot) (Shaw-Lee et al., 1991)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Tpi-PA   FBpp0084948   37.9   348   7.73     NP_788764   AAN14218 Tpi-PB   FBpp0084949   26.6   247   5.80     NP_788765   AAN14219 Tpi-PC   FBpp0084950   26.6   247   5.80     NP_788766   AAF57011
Additional Polypeptide Data & Comments
Reported size (kDa)	247 (aa) (Shaw-Lee et al., 1991)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Triosephosphate isomerase (IPR000652) Aldolase-type TIM barrel (IPR013785) Triosephosphate isomerase, active site (IPR020861) Triosephosphate isomerase, bacterial/eukaryotic (IPR022896)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA264270   AA264858   AA568006   AA696290   AA696741   AA697159   AA735236   AA802109   AE014297 AAF57011   AE014297 AAN14218   AE014297 AAN14219   AI108071   AI114163   AI134810   AI135947   AI258075   AI260349   AI260549   AI260618   AI293792   AI294600   AI295057   AI387546   AI389886   AI389995   AI402234   AY231717   BE662241   BE975258   BE975696   BE975697   BE976342   BE976634   BE977009   BF490886   BF491023   BF491308   BF491346   BF494024   BF494254   BF494517   BF494734   BF495174   BF496034   BF498877   BF499704   BF500029   BF502646   BF503960   BF504800   BF506038   BG632057   BG633770   BG634003   BG634726   BG634758   BI162116   BI162117   BI166361   BI166595   BI166979   BI168220   BI213690   BI215348   BI229131   BI231957   BI239613   BI240096   BI240898   BI353256   BI353702   BI354966   BI358461   BI364228   BI365810   BI375869   BI482589   BI482843   BI563375   BI564303   BI564676   BI566325   BI574899   BI575599   BI583108   BI583215   BI585751   BI587260   BI588228   BI605809   BI608664   BI612813   BI614243   BI619364   BI628432   BI628433   BI628770   BI630461   BI632322   BI634224   BI634441   BI634960   BI635647   BI636685   BI639742   BI640023   BI641284   BT012347   BT014664   BT029261   BX018846   BX066292   BX071751   CA804051   CK135300   CK570282   CK659066   CK660250   CL527709   CL868516   CO195468   CO263269   CO263275   CO266637   CO271445   CO300633   CO300712   CO319194   EC062485   EC071587   EC082133   EC085640   EC088435   EC215912   EC219605   EC231252   EC231253   EL881053   EL881166   EL881274   EL881396   EL886954   EL887378   ES696036   EV576136   EV577904   EV580850   U60836 AAC39041   U60837 AAC39042   U60838 AAC39043   U60839 AAC39044   U60840 AAC39045   U60841 AAC39046   U60842 AAC39047   U60843 AAC39048   U60844 AAC39049   U60845 AAC39050   U60846 AAC39051   U60847 AAC39052   U60848 AAC39053   U60849 AAC39054   U60850 AAC39055   U60851 AAC39056   U60852 AAC39057   U60853 AAC39058   U60854 AAC39059   U60855 AAC39060   U60856 AAC39061   U60857 AAC39062   U60858 AAC39063   U60859 AAC39064   U60860 AAC39065   X57576 CAA40804
UniProtKB/Swiss-Prot	P29613
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 13 -- 16   embryonic/larval muscle system   (Fisher et al., 2012) embryonic/larval somatic muscle   (Fisher et al., 2012) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage -- adult stage     (Shaw-Lee et al., 1991)
Additional Descriptive Data
	Tpi transcripts are detected throughout development on northern blots. They are very abundant in very early embryos and then decline. Levels then rise to a peak in third instar larvae followed by a decline and a subsequent rise near the time of eclosion. This is a pattern characteristic of enzymes involved in intermediate metabolism. (Shaw-Lee et al., 1991)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
enzyme assay or biochemical detection Stage Tissue/Position (including subcellular localization) Reference third instar larval stage   wing disc   (Santaren et al., 1993) immunolocalization Stage Tissue/Position (including subcellular localization) Reference adult stage   flight muscle   • myofibril | restricted (Sullivan et al., 2003) flight muscle   • sarcomere | restricted (Sullivan et al., 2003) flight muscle   • M band (Sullivan et al., 2003) flight muscle   • Z disc (Sullivan et al., 2003) mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Dorus et al., 2006) day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011)
Additional Descriptive Data
	Glycolytic enzymes, including Tpi protein are localized to M bands and Z discs, as visualized in the isolated myofibrils of flight muscles, where immunoreactivity appears as alternating bright and less-intense bands. (Sullivan et al., 2003) Tpi protein was identified in wing discs. (Santaren et al., 1993)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
M band inferred from direct assay (Sullivan et al., 2003) Z disc inferred from direct assay (Sullivan et al., 2003)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: High levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    2000.7 Larval Midgut    2387.2 Larval Hindgut    2037.8 Larval Malpighian Tubules    1934.7 Larval Fat Body    2362.3 Larval Salivary Gland    2524.5 Larval Trachea    2057.3 Larval Carcass    5079.425 Adult Head    2415.6 Adult Eye    3169.625 Adult Brain    1682.7 Adult Thoracic-Abdominal Ganglion    1763.2 Adult Crop    2642.6 Adult Midgut    2233.5 Adult Hindgut    3226.4 Adult Malpighian Tubules    2216.6 Adult Fat Body    3386.5 Adult Salivary Gland    5245.4 Adult Heart    3072.625 Adult VirginFemale Spermatheca    4059.9 Adult InseminatedFemale Spermatheca    3927.9 Adult Ovary    2048.6 Adult Testis    1804.4 Adult Male Accessory Gland    676.4 Adult Carcass    3219.5 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (2000.7) Larval Midgut  (2387.2) Larval Hindgut  (2037.8) Larval Malpighian Tubules  (1934.7) Larval Fat Body  (2362.3) Larval Salivary Gland  (2524.5) Larval Trachea  (2057.3) Larval Carcass  (5079.425) Adult Head  (2415.6) Adult Eye  (3169.625) Adult Brain  (1682.7) Adult Thoracic-Abdominal Ganglion  (1763.2) Adult Crop  (2642.6) Adult Midgut  (2233.5) Adult Hindgut  (3226.4) Adult Malpighian Tubules  (2216.6) Adult Fat Body  (3386.5) Adult Salivary Gland  (5245.4) Adult Heart  (3072.625) Adult VirginFemale Spermatheca  (4059.9) Adult InseminatedFemale Spermatheca  (3927.9) Adult Ovary  (2048.6) Adult Testis  (1804.4) Adult Male Accessory Gland  (676.4) Adult Carcass  (3219.5) Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  (2000.7) Larval Midgut  (2387.2) Larval Hindgut  (2037.8) Larval Malpighian Tubules  (1934.7) Larval Fat Body  (2362.3) Larval Salivary Gland  (2524.5) Larval Trachea  (2057.3) Larval Carcass  (5079.425) Adult Head  (2415.6) Adult Eye  (3169.625) Adult Brain  (1682.7) Adult Thoracic-Abdominal Ganglion  (1763.2) Adult Crop  (2642.6) Adult Midgut  (2233.5) Adult Hindgut  (3226.4) Adult Malpighian Tubules  (2216.6) Adult Fat Body  (3386.5) Adult Salivary Gland  (5245.4) Adult Heart  (3072.625) Adult VirginFemale Spermatheca  (4059.9) Adult InseminatedFemale Spermatheca  (3927.9) Adult Ovary  (2048.6) Adult Testis  (1804.4) Adult Male Accessory Gland    676.4 Adult Carcass  (3219.5) Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    2000.7 Larval Midgut    2387.2 Larval Hindgut    2037.8 Larval Malpighian Tubules    1934.7 Larval Fat Body    2362.3 Larval Salivary Gland    2524.5 Larval Trachea    2057.3 Larval Carcass    5079.425 Adult Head    2415.6 Adult Eye    3169.625 Adult Brain    1682.7 Adult Thoracic-Abdominal Ganglion    1763.2 Adult Crop    2642.6 Adult Midgut    2233.5 Adult Hindgut    3226.4 Adult Malpighian Tubules    2216.6 Adult Fat Body    3386.5 Adult Salivary Gland    5245.4 Adult Heart    3072.625 Adult VirginFemale Spermatheca    4059.9 Adult InseminatedFemale Spermatheca    3927.9 Adult Ovary    2048.6 Adult Testis    1804.4 Adult Male Accessory Gland    676.4 Adult Carcass    3219.5 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    2000.7 Larval Midgut    2387.2 Larval Hindgut    2037.8 Larval Malpighian Tubules    1934.7 Larval Fat Body    2362.3 Larval Salivary Gland    2524.5 Larval Trachea    2057.3 Larval Carcass    5079.425 Adult Head    2415.6 Adult Eye    3169.625 Adult Brain    1682.7 Adult Thoracic-Abdominal Ganglion    1763.2 Adult Crop    2642.6 Adult Midgut    2233.5 Adult Hindgut    3226.4 Adult Malpighian Tubules    2216.6 Adult Fat Body    3386.5 Adult Salivary Gland    5245.4 Adult Heart    3072.625 Adult VirginFemale Spermatheca    4059.9 Adult InseminatedFemale Spermatheca    3927.9 Adult Ovary    2048.6 Adult Testis    1804.4 Adult Male Accessory Gland    676.4 Adult Carcass    3219.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (2000.7) Larval Midgut  (2387.2) Larval Hindgut  (2037.8) Larval Malpighian Tubules  (1934.7) Larval Fat Body  (2362.3) Larval Salivary Gland  (2524.5) Larval Trachea  (2057.3) Larval Carcass  (5079.425) Adult Head  (2415.6) Adult Eye  (3169.625) Adult Brain  (1682.7) Adult Thoracic-Abdominal Ganglion  (1763.2) Adult Crop  (2642.6) Adult Midgut  (2233.5) Adult Hindgut  (3226.4) Adult Malpighian Tubules  (2216.6) Adult Fat Body  (3386.5) Adult Salivary Gland  (5245.4) Adult Heart  (3072.625) Adult VirginFemale Spermatheca  (4059.9) Adult InseminatedFemale Spermatheca  (3927.9) Adult Ovary  (2048.6) Adult Testis  (1804.4) Adult Male Accessory Gland  676.4 Adult Carcass  (3219.5) Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  (2000.7) Larval Midgut  (2387.2) Larval Hindgut  (2037.8) Larval Malpighian Tubules  (1934.7) Larval Fat Body  (2362.3) Larval Salivary Gland  (2524.5) Larval Trachea  (2057.3) Larval Carcass  (5079.425) Adult Head  (2415.6) Adult Eye  (3169.625) Adult Brain  1682.7 Adult Thoracic-Abdominal Ganglion  (1763.2) Adult Crop  (2642.6) Adult Midgut  (2233.5) Adult Hindgut  (3226.4) Adult Malpighian Tubules  (2216.6) Adult Fat Body  (3386.5) Adult Salivary Gland  (5245.4) Adult Heart  (3072.625) Adult VirginFemale Spermatheca  (4059.9) Adult InseminatedFemale Spermatheca  (3927.9) Adult Ovary  (2048.6) Adult Testis  (1804.4) Adult Male Accessory Gland    676.4 Adult Carcass  (3219.5) Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    2000.7 Larval Midgut    2387.2 Larval Hindgut    2037.8 Larval Malpighian Tubules    1934.7 Larval Fat Body    2362.3 Larval Salivary Gland    2524.5 Larval Trachea    2057.3 Larval Carcass    5079.425 Adult Head    2415.6 Adult Eye    3169.625 Adult Brain    1682.7 Adult Thoracic-Abdominal Ganglion    1763.2 Adult Crop    2642.6 Adult Midgut    2233.5 Adult Hindgut    3226.4 Adult Malpighian Tubules    2216.6 Adult Fat Body    3386.5 Adult Salivary Gland    5245.4 Adult Heart    3072.625 Adult VirginFemale Spermatheca    4059.9 Adult InseminatedFemale Spermatheca    3927.9 Adult Ovary    2048.6 Adult Testis    1804.4 Adult Male Accessory Gland    676.4 Adult Carcass    3219.5 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    2415.6   NA  Eye    3169.625   NA  Brain    1682.7   2000.7  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    1763.2   NA  Crop    2642.6   2387.2  Midgut    2233.5   2037.8  Hindgut    3226.4   1934.7  Malpighian Tubules    2216.6   2362.3  Fat Body    3386.5   2524.5  Salivary Gland    5245.4   NA  Heart    3072.625   2057.3  Trachea    NA   NA  VirginFemale Spermatheca    4059.9   NA  InseminatedFemale Spermatheca    3927.9   NA  Ovary    2048.6   NA  Testis    1804.4   NA  Male Accessory Gland    676.4   5079.425  Carcass    3219.5 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    257 central nervous system, larvae L3    67 central nervous system, pupae P8    43 head, virgin 1-day female    293 head, virgin 4-day female    166 head, virgin 20-day female    222 head, mated 1-day female    198 head, mated 4-day female    99 head, mated 20-day female    177 head, mated 1-day male    323 head, mated 4-day male    163 head, mated 20-day male    166 salivary gland, larvae L3 wandering    25 salivary gland, white prepupae    27 digestive system, larvae L3 wandering    37 digestive system, 1-day adult    210 digestive system, 4-day adult    121 digestive system, 20-day adult    148 fat body, larvae L3 wandering    268 fat body, white prepupae    114 fat body, pupae P8    73 carcass, larvae L3 wandering    155 carcass, 1-day adult    449 carcass, 4-day adult    181 carcass, 20-day adult    212 ovary, virgin 4-day female    258 ovary, mated 4-day female    260 testis, mated 4-day male    434 accessory gland, mated 4-day male    105 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (257) central nervous system, larvae L3  (67) central nervous system, pupae P8  (43) head, virgin 1-day female  (293) head, virgin 4-day female  (166) head, virgin 20-day female  (222) head, mated 1-day female  (198) head, mated 4-day female  (99) head, mated 20-day female  (177) head, mated 1-day male  (323) head, mated 4-day male  (163) head, mated 20-day male  (166) salivary gland, larvae L3 wandering    25 salivary gland, white prepupae  (27) digestive system, larvae L3 wandering  (37) digestive system, 1-day adult  (210) digestive system, 4-day adult  (121) digestive system, 20-day adult  (148) fat body, larvae L3 wandering  (268) fat body, white prepupae  (114) fat body, pupae P8  (73) carcass, larvae L3 wandering  (155) carcass, 1-day adult  (449) carcass, 4-day adult  (181) carcass, 20-day adult  (212) ovary, virgin 4-day female  (258) ovary, mated 4-day female  (260) testis, mated 4-day male  (434) accessory gland, mated 4-day male  (105) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (257) central nervous system, larvae L3    67 central nervous system, pupae P8    43 head, virgin 1-day female  (293) head, virgin 4-day female  (166) head, virgin 20-day female  (222) head, mated 1-day female  (198) head, mated 4-day female    99 head, mated 20-day female  (177) head, mated 1-day male  (323) head, mated 4-day male  (163) head, mated 20-day male  (166) salivary gland, larvae L3 wandering    25 salivary gland, white prepupae    27 digestive system, larvae L3 wandering    37 digestive system, 1-day adult  (210) digestive system, 4-day adult  (121) digestive system, 20-day adult  (148) fat body, larvae L3 wandering  (268) fat body, white prepupae  (114) fat body, pupae P8    73 carcass, larvae L3 wandering  (155) carcass, 1-day adult  (449) carcass, 4-day adult  (181) carcass, 20-day adult  (212) ovary, virgin 4-day female  (258) ovary, mated 4-day female  (260) testis, mated 4-day male  (434) accessory gland, mated 4-day male  105 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    257 central nervous system, larvae L3    67 central nervous system, pupae P8    43 head, virgin 1-day female    293 head, virgin 4-day female    166 head, virgin 20-day female    222 head, mated 1-day female    198 head, mated 4-day female    99 head, mated 20-day female    177 head, mated 1-day male    323 head, mated 4-day male    163 head, mated 20-day male    166 salivary gland, larvae L3 wandering    25 salivary gland, white prepupae    27 digestive system, larvae L3 wandering    37 digestive system, 1-day adult    210 digestive system, 4-day adult    121 digestive system, 20-day adult    148 fat body, larvae L3 wandering    268 fat body, white prepupae    114 fat body, pupae P8    73 carcass, larvae L3 wandering    155 carcass, 1-day adult    449 carcass, 4-day adult    181 carcass, 20-day adult    212 ovary, virgin 4-day female    258 ovary, mated 4-day female    260 testis, mated 4-day male    434 accessory gland, mated 4-day male    105 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    257 central nervous system, larvae L3    67 central nervous system, pupae P8    43 head, virgin 1-day female    293 head, virgin 4-day female    166 head, virgin 20-day female    222 head, mated 1-day female    198 head, mated 4-day female    99 head, mated 20-day female    177 head, mated 1-day male    323 head, mated 4-day male    163 head, mated 20-day male    166 salivary gland, larvae L3 wandering    25 salivary gland, white prepupae    27 digestive system, larvae L3 wandering    37 digestive system, 1-day adult    210 digestive system, 4-day adult    121 digestive system, 20-day adult    148 fat body, larvae L3 wandering    268 fat body, white prepupae    114 fat body, pupae P8    73 carcass, larvae L3 wandering    155 carcass, 1-day adult    449 carcass, 4-day adult    181 carcass, 20-day adult    212 ovary, virgin 4-day female    258 ovary, mated 4-day female    260 testis, mated 4-day male    434 accessory gland, mated 4-day male    105 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (257) central nervous system, larvae L3  (67) central nervous system, pupae P8  (43) head, virgin 1-day female  (293) head, virgin 4-day female  (166) head, virgin 20-day female  (222) head, mated 1-day female  (198) head, mated 4-day female  (99) head, mated 20-day female  (177) head, mated 1-day male  (323) head, mated 4-day male  (163) head, mated 20-day male  (166) salivary gland, larvae L3 wandering    25 salivary gland, white prepupae  27 digestive system, larvae L3 wandering  (37) digestive system, 1-day adult  (210) digestive system, 4-day adult  (121) digestive system, 20-day adult  (148) fat body, larvae L3 wandering  (268) fat body, white prepupae  (114) fat body, pupae P8  (73) carcass, larvae L3 wandering  (155) carcass, 1-day adult  (449) carcass, 4-day adult  (181) carcass, 20-day adult  (212) ovary, virgin 4-day female  (258) ovary, mated 4-day female  (260) testis, mated 4-day male  (434) accessory gland, mated 4-day male  (105) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (257) central nervous system, larvae L3    67 central nervous system, pupae P8    43 head, virgin 1-day female  (293) head, virgin 4-day female  (166) head, virgin 20-day female  (222) head, mated 1-day female  (198) head, mated 4-day female    99 head, mated 20-day female  (177) head, mated 1-day male  (323) head, mated 4-day male  (163) head, mated 20-day male  (166) salivary gland, larvae L3 wandering    25 salivary gland, white prepupae    27 digestive system, larvae L3 wandering    37 digestive system, 1-day adult  (210) digestive system, 4-day adult  121 digestive system, 20-day adult  (148) fat body, larvae L3 wandering  (268) fat body, white prepupae  114 fat body, pupae P8    73 carcass, larvae L3 wandering  (155) carcass, 1-day adult  (449) carcass, 4-day adult  (181) carcass, 20-day adult  (212) ovary, virgin 4-day female  (258) ovary, mated 4-day female  (260) testis, mated 4-day male  (434) accessory gland, mated 4-day male  105 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    257 central nervous system, larvae L3    67 central nervous system, pupae P8    43 head, virgin 1-day female    293 head, virgin 4-day female    166 head, virgin 20-day female    222 head, mated 1-day female    198 head, mated 4-day female    99 head, mated 20-day female    177 head, mated 1-day male    323 head, mated 4-day male    163 head, mated 20-day male    166 salivary gland, larvae L3 wandering    25 salivary gland, white prepupae    27 digestive system, larvae L3 wandering    37 digestive system, 1-day adult    210 digestive system, 4-day adult    121 digestive system, 20-day adult    148 fat body, larvae L3 wandering    268 fat body, white prepupae    114 fat body, pupae P8    73 carcass, larvae L3 wandering    155 carcass, 1-day adult    449 carcass, 4-day adult    181 carcass, 20-day adult    212 ovary, virgin 4-day female    258 ovary, mated 4-day female    260 testis, mated 4-day male    434 accessory gland, mated 4-day male    105 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed within 00-06 and 12-24 hour embryonic stages, at stages throughout the larval period, during late pupal stages, in stages of adults of both sexes. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    321 embryo 02-04hr    7 embryo 04-06hr    4 embryo 06-08hr    3 embryo 08-10hr    5 embryo 10-12hr    20 embryo 12-14hr    29 embryo 14-16hr    138 embryo 16-18hr    158 embryo 18-20hr    251 embryo 20-22hr    272 embryo 22-24hr    241 larva L1    406 larva L2    455 larva L3 12hr old    575 larva L3 puffstage 1-2    575 larva L3 puffstage 3-6    316 larva L3 puffstage 7-9    142 white prepupae new    120 white prepupae 12hr    84 white prepupae 24hr    113 pupae 2d postWPP    181 pupae 3d postWPP    370 pupae 4d postWPP    471 adult male 01day    491 adult male 05day    375 adult male 30day    271 adult female 01day    453 adult female 05day    252 adult female 30day    265 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (321) embryo 02-04hr    7 embryo 04-06hr    4 embryo 06-08hr    3 embryo 08-10hr    5 embryo 10-12hr    20 embryo 12-14hr  (29) embryo 14-16hr  (138) embryo 16-18hr  (158) embryo 18-20hr  (251) embryo 20-22hr  (272) embryo 22-24hr  (241) larva L1  (406) larva L2  (455) larva L3 12hr old  (575) larva L3 puffstage 1-2  (575) larva L3 puffstage 3-6  (316) larva L3 puffstage 7-9  (142) white prepupae new  (120) white prepupae 12hr  (84) white prepupae 24hr  (113) pupae 2d postWPP  (181) pupae 3d postWPP  (370) pupae 4d postWPP  (471) adult male 01day  (491) adult male 05day  (375) adult male 30day  (271) adult female 01day  (453) adult female 05day  (252) adult female 30day  (265) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (321) embryo 02-04hr    7 embryo 04-06hr    4 embryo 06-08hr    3 embryo 08-10hr    5 embryo 10-12hr    20 embryo 12-14hr    29 embryo 14-16hr  (138) embryo 16-18hr  (158) embryo 18-20hr  (251) embryo 20-22hr  (272) embryo 22-24hr  (241) larva L1  (406) larva L2  (455) larva L3 12hr old  (575) larva L3 puffstage 1-2  (575) larva L3 puffstage 3-6  (316) larva L3 puffstage 7-9  (142) white prepupae new  (120) white prepupae 12hr    84 white prepupae 24hr  (113) pupae 2d postWPP  (181) pupae 3d postWPP  (370) pupae 4d postWPP  (471) adult male 01day  (491) adult male 05day  (375) adult male 30day  (271) adult female 01day  (453) adult female 05day  (252) adult female 30day  (265) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    321 embryo 02-04hr    7 embryo 04-06hr    4 embryo 06-08hr    3 embryo 08-10hr    5 embryo 10-12hr    20 embryo 12-14hr    29 embryo 14-16hr    138 embryo 16-18hr    158 embryo 18-20hr    251 embryo 20-22hr    272 embryo 22-24hr    241 larva L1    406 larva L2    455 larva L3 12hr old    575 larva L3 puffstage 1-2    575 larva L3 puffstage 3-6    316 larva L3 puffstage 7-9    142 white prepupae new    120 white prepupae 12hr    84 white prepupae 24hr    113 pupae 2d postWPP    181 pupae 3d postWPP    370 pupae 4d postWPP    471 adult male 01day    491 adult male 05day    375 adult male 30day    271 adult female 01day    453 adult female 05day    252 adult female 30day    265 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    321 embryo 02-04hr    7 embryo 04-06hr    4 embryo 06-08hr    3 embryo 08-10hr    5 embryo 10-12hr    20 embryo 12-14hr    29 embryo 14-16hr    138 embryo 16-18hr    158 embryo 18-20hr    251 embryo 20-22hr    272 embryo 22-24hr    241 larva L1    406 larva L2    455 larva L3 12hr old    575 larva L3 puffstage 1-2    575 larva L3 puffstage 3-6    316 larva L3 puffstage 7-9    142 white prepupae new    120 white prepupae 12hr    84 white prepupae 24hr    113 pupae 2d postWPP    181 pupae 3d postWPP    370 pupae 4d postWPP    471 adult male 01day    491 adult male 05day    375 adult male 30day    271 adult female 01day    453 adult female 05day    252 adult female 30day    265 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (321) embryo 02-04hr    7 embryo 04-06hr    4 embryo 06-08hr    3 embryo 08-10hr    5 embryo 10-12hr    20 embryo 12-14hr  29 embryo 14-16hr  (138) embryo 16-18hr  (158) embryo 18-20hr  (251) embryo 20-22hr  (272) embryo 22-24hr  (241) larva L1  (406) larva L2  (455) larva L3 12hr old  (575) larva L3 puffstage 1-2  (575) larva L3 puffstage 3-6  (316) larva L3 puffstage 7-9  (142) white prepupae new  (120) white prepupae 12hr  (84) white prepupae 24hr  (113) pupae 2d postWPP  (181) pupae 3d postWPP  (370) pupae 4d postWPP  (471) adult male 01day  (491) adult male 05day  (375) adult male 30day  (271) adult female 01day  (453) adult female 05day  (252) adult female 30day  (265) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (321) embryo 02-04hr    7 embryo 04-06hr    4 embryo 06-08hr    3 embryo 08-10hr    5 embryo 10-12hr    20 embryo 12-14hr    29 embryo 14-16hr  138 embryo 16-18hr  (158) embryo 18-20hr  (251) embryo 20-22hr  (272) embryo 22-24hr  (241) larva L1  (406) larva L2  (455) larva L3 12hr old  (575) larva L3 puffstage 1-2  (575) larva L3 puffstage 3-6  (316) larva L3 puffstage 7-9  142 white prepupae new  120 white prepupae 12hr    84 white prepupae 24hr  113 pupae 2d postWPP  (181) pupae 3d postWPP  (370) pupae 4d postWPP  (471) adult male 01day  (491) adult male 05day  (375) adult male 30day  (271) adult female 01day  (453) adult female 05day  (252) adult female 30day  (265) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    321 embryo 02-04hr    7 embryo 04-06hr    4 embryo 06-08hr    3 embryo 08-10hr    5 embryo 10-12hr    20 embryo 12-14hr    29 embryo 14-16hr    138 embryo 16-18hr    158 embryo 18-20hr    251 embryo 20-22hr    272 embryo 22-24hr    241 larva L1    406 larva L2    455 larva L3 12hr old    575 larva L3 puffstage 1-2    575 larva L3 puffstage 3-6    316 larva L3 puffstage 7-9    142 white prepupae new    120 white prepupae 12hr    84 white prepupae 24hr    113 pupae 2d postWPP    181 pupae 3d postWPP    370 pupae 4d postWPP    471 adult male 01day    491 adult male 05day    375 adult male 30day    271 adult female 01day    453 adult female 05day    252 adult female 30day    265 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    65 Schneider line 2 Sg4    38 embryonic 1182-4H    75 embryonic GM2    26 embryonic Kc167    178 embryonic S1    67 embryonic S3    31 leg disc CME L1    52 wing disc CME-W2    54 wing disc ML-DmD8    215 wing disc ML-DmD9    135 wing disc ML-DmD16-c3    128 wing disc ML-DmD21    107 wing disc ML-DmD32    70 haltere disc ML-DmD17-c3    96 eye-antennal disc ML-DmD11    66 antennal disc ML-DmD20-c5    52 mixed discs ML-DmD4-c1    143 CNS ML-DmBG1-c1    261 CNS ML-DmBG2-c2    95 tumorous blood cells mbn2    120 ovary fGS/OSS    147 ovary OSC    50 ovary OSS    36 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (65) Schneider line 2 Sg4  (38) embryonic 1182-4H  (75) embryonic GM2  26 embryonic Kc167  (178) embryonic S1  (67) embryonic S3  (31) leg disc CME L1  (52) wing disc CME-W2  (54) wing disc ML-DmD8  (215) wing disc ML-DmD9  (135) wing disc ML-DmD16-c3  (128) wing disc ML-DmD21  (107) wing disc ML-DmD32  (70) haltere disc ML-DmD17-c3  (96) eye-antennal disc ML-DmD11  (66) antennal disc ML-DmD20-c5  (52) mixed discs ML-DmD4-c1  (143) CNS ML-DmBG1-c1  (261) CNS ML-DmBG2-c2  (95) tumorous blood cells mbn2  (120) ovary fGS/OSS  (147) ovary OSC  (50) ovary OSS  (36) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    65 Schneider line 2 Sg4    38 embryonic 1182-4H    75 embryonic GM2    26 embryonic Kc167  (178) embryonic S1    67 embryonic S3    31 leg disc CME L1    52 wing disc CME-W2    54 wing disc ML-DmD8  (215) wing disc ML-DmD9  (135) wing disc ML-DmD16-c3  (128) wing disc ML-DmD21  107 wing disc ML-DmD32    70 haltere disc ML-DmD17-c3    96 eye-antennal disc ML-DmD11    66 antennal disc ML-DmD20-c5    52 mixed discs ML-DmD4-c1  (143) CNS ML-DmBG1-c1  (261) CNS ML-DmBG2-c2    95 tumorous blood cells mbn2  (120) ovary fGS/OSS  (147) ovary OSC    50 ovary OSS    36 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    65 Schneider line 2 Sg4    38 embryonic 1182-4H    75 embryonic GM2    26 embryonic Kc167    178 embryonic S1    67 embryonic S3    31 leg disc CME L1    52 wing disc CME-W2    54 wing disc ML-DmD8    215 wing disc ML-DmD9    135 wing disc ML-DmD16-c3    128 wing disc ML-DmD21    107 wing disc ML-DmD32    70 haltere disc ML-DmD17-c3    96 eye-antennal disc ML-DmD11    66 antennal disc ML-DmD20-c5    52 mixed discs ML-DmD4-c1    143 CNS ML-DmBG1-c1    261 CNS ML-DmBG2-c2    95 tumorous blood cells mbn2    120 ovary fGS/OSS    147 ovary OSC    50 ovary OSS    36 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    65 Schneider line 2 Sg4    38 embryonic 1182-4H    75 embryonic GM2    26 embryonic Kc167    178 embryonic S1    67 embryonic S3    31 leg disc CME L1    52 wing disc CME-W2    54 wing disc ML-DmD8    215 wing disc ML-DmD9    135 wing disc ML-DmD16-c3    128 wing disc ML-DmD21    107 wing disc ML-DmD32    70 haltere disc ML-DmD17-c3    96 eye-antennal disc ML-DmD11    66 antennal disc ML-DmD20-c5    52 mixed discs ML-DmD4-c1    143 CNS ML-DmBG1-c1    261 CNS ML-DmBG2-c2    95 tumorous blood cells mbn2    120 ovary fGS/OSS    147 ovary OSC    50 ovary OSS    36 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (65) Schneider line 2 Sg4  (38) embryonic 1182-4H  (75) embryonic GM2  26 embryonic Kc167  (178) embryonic S1  (67) embryonic S3  31 leg disc CME L1  (52) wing disc CME-W2  (54) wing disc ML-DmD8  (215) wing disc ML-DmD9  (135) wing disc ML-DmD16-c3  (128) wing disc ML-DmD21  (107) wing disc ML-DmD32  (70) haltere disc ML-DmD17-c3  (96) eye-antennal disc ML-DmD11  (66) antennal disc ML-DmD20-c5  (52) mixed discs ML-DmD4-c1  (143) CNS ML-DmBG1-c1  (261) CNS ML-DmBG2-c2  (95) tumorous blood cells mbn2  (120) ovary fGS/OSS  (147) ovary OSC  (50) ovary OSS  (36) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    65 Schneider line 2 Sg4    38 embryonic 1182-4H    75 embryonic GM2    26 embryonic Kc167  (178) embryonic S1    67 embryonic S3    31 leg disc CME L1    52 wing disc CME-W2    54 wing disc ML-DmD8  (215) wing disc ML-DmD9  135 wing disc ML-DmD16-c3  128 wing disc ML-DmD21  107 wing disc ML-DmD32    70 haltere disc ML-DmD17-c3    96 eye-antennal disc ML-DmD11    66 antennal disc ML-DmD20-c5    52 mixed discs ML-DmD4-c1  143 CNS ML-DmBG1-c1  (261) CNS ML-DmBG2-c2    95 tumorous blood cells mbn2  120 ovary fGS/OSS  (147) ovary OSC    50 ovary OSS    36 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    65 Schneider line 2 Sg4    38 embryonic 1182-4H    75 embryonic GM2    26 embryonic Kc167    178 embryonic S1    67 embryonic S3    31 leg disc CME L1    52 wing disc CME-W2    54 wing disc ML-DmD8    215 wing disc ML-DmD9    135 wing disc ML-DmD16-c3    128 wing disc ML-DmD21    107 wing disc ML-DmD32    70 haltere disc ML-DmD17-c3    96 eye-antennal disc ML-DmD11    66 antennal disc ML-DmD20-c5    52 mixed discs ML-DmD4-c1    143 CNS ML-DmBG1-c1    261 CNS ML-DmBG2-c2    95 tumorous blood cells mbn2    120 ovary fGS/OSS    147 ovary OSC    50 ovary OSS    36 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    186 cold shock, 4-day adult    125 heat shock, 4-day adult    646 Cadmium 50 mM 6 hrs, larvae L3    1097 Cadmium 50 mM 12 hrs, larvae L3    515 Cadmium 50 mM 48 hrs, 4-day adult    551 Cadmium 100 mM 48 hrs, 4-day adult    232 Copper 0.5 mM 12 hrs, larvae L3    953 Copper 15 mM 48 hrs, 4-day adult    44 Zinc 5 mM 12 hrs, larvae L3    442 Zinc 4.5 mM 48 hrs, 4-day adult    85 Ethanol 2.5% 3 hrs, larvae L3    451 Ethanol 5% 3 hrs, larvae L3    613 Ethanol 10% 3 hrs, larvae L3    1156 Caffeine 1.5 mg/ml 4 hrs, larvae L3    580 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    209 Caffeine 25 mg/ml 48 hrs, 4-day adult    154 Paraquat 5 mM 48 hrs, 4-day adult    311 Paraquat 10 mM 48 hrs, 4-day adult    490 Rotenone 2 μg 12 hrs, larvae L3    793 Rotenone 8 μg 12 hrs, larvae L3    1145 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (186) cold shock, 4-day adult  (125) heat shock, 4-day adult  (646) Cadmium 50 mM 6 hrs, larvae L3  (1097) Cadmium 50 mM 12 hrs, larvae L3  (515) Cadmium 50 mM 48 hrs, 4-day adult  (551) Cadmium 100 mM 48 hrs, 4-day adult  (232) Copper 0.5 mM 12 hrs, larvae L3  (953) Copper 15 mM 48 hrs, 4-day adult  (44) Zinc 5 mM 12 hrs, larvae L3  (442) Zinc 4.5 mM 48 hrs, 4-day adult  (85) Ethanol 2.5% 3 hrs, larvae L3  (451) Ethanol 5% 3 hrs, larvae L3  (613) Ethanol 10% 3 hrs, larvae L3  (1156) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (580) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (209) Caffeine 25 mg/ml 48 hrs, 4-day adult  (154) Paraquat 5 mM 48 hrs, 4-day adult  (311) Paraquat 10 mM 48 hrs, 4-day adult  (490) Rotenone 2 μg 12 hrs, larvae L3  (793) Rotenone 8 μg 12 hrs, larvae L3  (1145) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult  (186) cold shock, 4-day adult  (125) heat shock, 4-day adult  (646) Cadmium 50 mM 6 hrs, larvae L3  (1097) Cadmium 50 mM 12 hrs, larvae L3  (515) Cadmium 50 mM 48 hrs, 4-day adult  (551) Cadmium 100 mM 48 hrs, 4-day adult  (232) Copper 0.5 mM 12 hrs, larvae L3  (953) Copper 15 mM 48 hrs, 4-day adult    44 Zinc 5 mM 12 hrs, larvae L3  (442) Zinc 4.5 mM 48 hrs, 4-day adult    85 Ethanol 2.5% 3 hrs, larvae L3  (451) Ethanol 5% 3 hrs, larvae L3  (613) Ethanol 10% 3 hrs, larvae L3  (1156) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (580) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (209) Caffeine 25 mg/ml 48 hrs, 4-day adult  (154) Paraquat 5 mM 48 hrs, 4-day adult  (311) Paraquat 10 mM 48 hrs, 4-day adult  (490) Rotenone 2 μg 12 hrs, larvae L3  (793) Rotenone 8 μg 12 hrs, larvae L3  (1145) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    186 cold shock, 4-day adult    125 heat shock, 4-day adult    646 Cadmium 50 mM 6 hrs, larvae L3    1097 Cadmium 50 mM 12 hrs, larvae L3    515 Cadmium 50 mM 48 hrs, 4-day adult    551 Cadmium 100 mM 48 hrs, 4-day adult    232 Copper 0.5 mM 12 hrs, larvae L3    953 Copper 15 mM 48 hrs, 4-day adult    44 Zinc 5 mM 12 hrs, larvae L3    442 Zinc 4.5 mM 48 hrs, 4-day adult    85 Ethanol 2.5% 3 hrs, larvae L3    451 Ethanol 5% 3 hrs, larvae L3    613 Ethanol 10% 3 hrs, larvae L3    1156 Caffeine 1.5 mg/ml 4 hrs, larvae L3    580 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    209 Caffeine 25 mg/ml 48 hrs, 4-day adult    154 Paraquat 5 mM 48 hrs, 4-day adult    311 Paraquat 10 mM 48 hrs, 4-day adult    490 Rotenone 2 μg 12 hrs, larvae L3    793 Rotenone 8 μg 12 hrs, larvae L3    1145 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    186 cold shock, 4-day adult    125 heat shock, 4-day adult    646 Cadmium 50 mM 6 hrs, larvae L3    1097 Cadmium 50 mM 12 hrs, larvae L3    515 Cadmium 50 mM 48 hrs, 4-day adult    551 Cadmium 100 mM 48 hrs, 4-day adult    232 Copper 0.5 mM 12 hrs, larvae L3    953 Copper 15 mM 48 hrs, 4-day adult    44 Zinc 5 mM 12 hrs, larvae L3    442 Zinc 4.5 mM 48 hrs, 4-day adult    85 Ethanol 2.5% 3 hrs, larvae L3    451 Ethanol 5% 3 hrs, larvae L3    613 Ethanol 10% 3 hrs, larvae L3    1156 Caffeine 1.5 mg/ml 4 hrs, larvae L3    580 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    209 Caffeine 25 mg/ml 48 hrs, 4-day adult    154 Paraquat 5 mM 48 hrs, 4-day adult    311 Paraquat 10 mM 48 hrs, 4-day adult    490 Rotenone 2 μg 12 hrs, larvae L3    793 Rotenone 8 μg 12 hrs, larvae L3    1145 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (186) cold shock, 4-day adult  (125) heat shock, 4-day adult  (646) Cadmium 50 mM 6 hrs, larvae L3  (1097) Cadmium 50 mM 12 hrs, larvae L3  (515) Cadmium 50 mM 48 hrs, 4-day adult  (551) Cadmium 100 mM 48 hrs, 4-day adult  (232) Copper 0.5 mM 12 hrs, larvae L3  (953) Copper 15 mM 48 hrs, 4-day adult  (44) Zinc 5 mM 12 hrs, larvae L3  (442) Zinc 4.5 mM 48 hrs, 4-day adult  (85) Ethanol 2.5% 3 hrs, larvae L3  (451) Ethanol 5% 3 hrs, larvae L3  (613) Ethanol 10% 3 hrs, larvae L3  (1156) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (580) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (209) Caffeine 25 mg/ml 48 hrs, 4-day adult  (154) Paraquat 5 mM 48 hrs, 4-day adult  (311) Paraquat 10 mM 48 hrs, 4-day adult  (490) Rotenone 2 μg 12 hrs, larvae L3  (793) Rotenone 8 μg 12 hrs, larvae L3  (1145) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult  (186) cold shock, 4-day adult  125 heat shock, 4-day adult  (646) Cadmium 50 mM 6 hrs, larvae L3  (1097) Cadmium 50 mM 12 hrs, larvae L3  (515) Cadmium 50 mM 48 hrs, 4-day adult  (551) Cadmium 100 mM 48 hrs, 4-day adult  (232) Copper 0.5 mM 12 hrs, larvae L3  (953) Copper 15 mM 48 hrs, 4-day adult    44 Zinc 5 mM 12 hrs, larvae L3  (442) Zinc 4.5 mM 48 hrs, 4-day adult    85 Ethanol 2.5% 3 hrs, larvae L3  (451) Ethanol 5% 3 hrs, larvae L3  (613) Ethanol 10% 3 hrs, larvae L3  (1156) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (580) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (209) Caffeine 25 mg/ml 48 hrs, 4-day adult  (154) Paraquat 5 mM 48 hrs, 4-day adult  (311) Paraquat 10 mM 48 hrs, 4-day adult  (490) Rotenone 2 μg 12 hrs, larvae L3  (793) Rotenone 8 μg 12 hrs, larvae L3  (1145) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    186 cold shock, 4-day adult    125 heat shock, 4-day adult    646 Cadmium 50 mM 6 hrs, larvae L3    1097 Cadmium 50 mM 12 hrs, larvae L3    515 Cadmium 50 mM 48 hrs, 4-day adult    551 Cadmium 100 mM 48 hrs, 4-day adult    232 Copper 0.5 mM 12 hrs, larvae L3    953 Copper 15 mM 48 hrs, 4-day adult    44 Zinc 5 mM 12 hrs, larvae L3    442 Zinc 4.5 mM 48 hrs, 4-day adult    85 Ethanol 2.5% 3 hrs, larvae L3    451 Ethanol 5% 3 hrs, larvae L3    613 Ethanol 10% 3 hrs, larvae L3    1156 Caffeine 1.5 mg/ml 4 hrs, larvae L3    580 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    209 Caffeine 25 mg/ml 48 hrs, 4-day adult    154 Paraquat 5 mM 48 hrs, 4-day adult    311 Paraquat 10 mM 48 hrs, 4-day adult    490 Rotenone 2 μg 12 hrs, larvae L3    793 Rotenone 8 μg 12 hrs, larvae L3    1145 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_10 mE1_20_mRNA_expression_cluster_10
External Data & Images
Linkouts	BDGP expression data • GH10864 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0086355 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG2171-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Tpi Stages(s) 1-3
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | recessive Tpisgk-js10 viable TpiEY03361 TpiEY11806 Tpisgk-1 Tpisgk-R1 Tpisgk-R2 Tpisgk-R3 Tpisgk-R4 viable, with Scer\GAL4pnr-MD237 TpiGD10138 Sterility Allele fertile TpiEY03361 TpiEY11806 Other Phenotypes Allele bang sensitive Tpisgk-1 bang sensitive | heat sensitive (with Tpisgk-1) Tpisgk-js10 bang sensitive | heat sensitive (with Tpisgk-js10) Tpisgk-1 electrophoretic variant | fast Tpi6 electrophoretic variant | slow Tpi4 neuroanatomy defective Tpisgk-1 neuroanatomy defective (with Tpisgk-1) Tpisgk-js10 neuroanatomy defective (with Tpisgk-js10) Tpisgk-1 short lived Tpisgk-1 short lived, with Scer\GAL4Act5C.PI TpiScer\UAS.cCa short lived | heat sensitive (with Tpisgk-1) Tpisgk-js10 short lived | heat sensitive (with Tpisgk-js10) Tpisgk-1 Phenotype manifest in Allele adult brain Tpisgk-1 adult brain (with Tpisgk-1) Tpisgk-js10 adult brain (with Tpisgk-js10) Tpisgk-1 central nervous system Tpisgk-1 thoracico-abdominal ganglion (with Tpisgk-1) Tpisgk-js10 thoracico-abdominal ganglion (with Tpisgk-js10) Tpisgk-1
Classical Alleles ( 15 )
For All Classical Alleles Show
Allele of Tpi Class Mutagen Stocks Known lesion Tpi4 natural population 2 -- Tpi6 natural population 2 -- Tpi5-HA-1094 1 -- TpiEY03361 P-element activity 1 -- TpiEY11806 P-element activity 1 -- TpiNP1356 1 -- TpiNP5917 1 -- Tpisgk-1 hypomorphic allele - genetic evidence 0 Yes Tpisgk-js10 Delta2-3 0 Yes Tpisgk-R1 Delta2-3 0 Yes Tpisgk-R2 Delta2-3 0 Yes Tpisgk-R3 Delta2-3 0 Yes Tpisgk-R4 Delta2-3 0 Yes Tpiwstd-1 ethyl methanesulfonate 0 -- Tpiwstd-2 0 --
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Tpi Class Mutagen Stocks Known lesion TpiGD10138 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes TpiJF02074 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes TpiNIG.2171R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes TpiScer\UAS.cCa in vitro construct - regulatory fusion 0 Yes Tpisgk-1.Scer\UAS in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Dp(3;1)1A (Frisardi and MacIntyre, 1984) Dp(3;1)19 (Frisardi and MacIntyre, 1984) Dp(3;1)27 (Frisardi and MacIntyre, 1984) Dp(3;1)48 (Frisardi and MacIntyre, 1984) Dp(3;1)79 (Frisardi and MacIntyre, 1984) Dp(3;1)88 (Frisardi and MacIntyre, 1984) Dp(3;1)93 (Frisardi and MacIntyre, 1984) Dp(3;1)138P (Frisardi and MacIntyre, 1984) Dp(3;1)150P (Frisardi and MacIntyre, 1984) Dp(3;1)152P (Frisardi and MacIntyre, 1984)
Duplicated in	Dp(3;Y)L127 (Frisardi and MacIntyre, 1984)
Not disrupted in	Dp(3;1)3 (Frisardi and MacIntyre, 1984) Dp(3;1)34 (Frisardi and MacIntyre, 1984) Dp(3;1)35 (Frisardi and MacIntyre, 1984) Dp(3;1)45B (Frisardi and MacIntyre, 1984) Dp(3;1)46A (Frisardi and MacIntyre, 1984) Dp(3;1)52 (Frisardi and MacIntyre, 1984) Dp(3;1)67A (Frisardi and MacIntyre, 1984) Dp(3;1)67N (Frisardi and MacIntyre, 1984) Dp(3;1)74 (Frisardi and MacIntyre, 1984) Dp(3;1)78 (Frisardi and MacIntyre, 1984) Dp(3;1)97 (Frisardi and MacIntyre, 1984) Dp(3;1)124P (Frisardi and MacIntyre, 1984) Dp(3;1)165P (Frisardi and MacIntyre, 1984) Dp(3;1)R10 (Frisardi and MacIntyre, 1984) Dp(3;1)R14 (Frisardi and MacIntyre, 1984)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD10138} NA P{NIG.2171R} NA P{TRiP.JF02074} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}TpiEY03361 NA P{EPgy2}TpiEY11806 NA miscellaneous insertions P{RS5}Tpi5-HA-1094 NA P{SUPor-P}KG03964 NA insertion of enhancer trap binary system P{GawB}TpiNP1356 No P{GawB}TpiNP5917 No
Gene Ontology: Function, Process & Cellular Component ( 7 unique terms )
Terms Based on Experimental Evidence ( 6 terms )
Molecular Function
CV term Evidence References
triose-phosphate isomerase activity inferred from mutant phenotype (Eanes et al., 2006)
Biological Process
CV term Evidence References
determination of adult lifespan inferred from mutant phenotype (Celotto et al., 2006) neurological system process inferred from mutant phenotype (Celotto et al., 2006) response to mechanical stimulus inferred from mutant phenotype (Celotto et al., 2006)
Cellular Component
CV term Evidence References
M band inferred from direct assay (Sullivan et al., 2003) Z disc inferred from direct assay (Sullivan et al., 2003)
Terms Based on Predictions or Assertions ( 2 terms )
Molecular Function
CV term Evidence References
triose-phosphate isomerase activity inferred from sequence or structural similarity with UniProtKB:P30741 (FlyBase, 1992-) non-traceable author statement (Swiss-Prot Project Members, 1993.4.1)
Biological Process
CV term Evidence References
glycolysis inferred from electronic annotation with InterPro:IPR022896 (FlyBase Curators et al., 2004-)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	gene   nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Tpi - CG10077 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Tpi - CG1129 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Tpi - CG31961 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Tpi - CG42784 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Tpi - CG5746 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Tpi - CG6330 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Tpi - CG7049 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Tpi - CG7889 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Tpi - CG9977 experimental knowledge based (Guruharsha et al., 2011) Tpi - Ccs anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Tpi - Cg25C anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Tpi - Chc anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Tpi - Eig71Ed anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Tpi - Mapmodulin experimental knowledge based (Guruharsha et al., 2011) Tpi - Npc2g anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Tpi - Obp19d experimental knowledge based (Guruharsha et al., 2011) Tpi - Pez experimental knowledge based (Guruharsha et al., 2011) Tpi - RpS21 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Tpi - Rpb8 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Tpi - Syx6 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Tpi - antdh experimental knowledge based (Guruharsha et al., 2011) Tpi - cathD anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Tpi - gish anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Tpi - grsm experimental knowledge based (Guruharsha et al., 2011) Tpi - pr experimental knowledge based (Guruharsha et al., 2011) Tpi - snama anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Tpi allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 68438 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0086355 DroID - A comprehensive database of gene and protein interactions. • FBgn0086355 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43582
Orthologs
OrthoDB Orthologs (38) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6VQB8C Dipteran inclusive ortholog search EOG6VHKG8 Insect inclusive ortholog search EOG6RBPFD Arthropod inclusive ortholog search EOG680GCC Metazoa inclusive ortholog search EOG67X1GJ
Orthologs in Drosophila Species (EOG6VQB8C)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Tpi     Drosophila simulans   Dsim\Tpi     Drosophila sechellia   Dsec\GM12179     Drosophila erecta   Dere\GG11962     Drosophila yakuba   Dyak\Tpi     Drosophila ananassae   Dana\GF16201     Drosophila pseudoobscura pseudoobscura   Dpse\Tpi     Drosophila persimilis   Dper\GL14069     Drosophila willistoni   Dwil\GK13151     Drosophila virilis   Dvir\GJ10377     Drosophila mojavensis   Dmoj\GI23383     Drosophila grimshawi   Dgri\GH18391
Orthologs in non-Drosophila Dipterans (EOG6VHKG8)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL002542   Anopheles gambiae Malaria mosquito  Agam\AGAP001630   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ002354
Orthologs in non-Dipteran Insects (EOG6RBPFD)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB54369   Apis mellifera Western honey bee  Amel\GB54370   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG017420   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH10515   Atta cephalotes Leafcutter ant  Acep\ACEP23283   Camponotus floridanus Florida carpenter ant  Cflo\CFLO14030   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL20610   Linepithema humile Argentine ant  Lhum\LH16840   Pogonomyrmex barbatus Red harvester ant  Pbar\PB12314   Acyrthosiphon pisum Pea aphid  Apim\ACYPI006727   Bombyx mori Silkmoth  Bmor\BGIBMGA000559   Pediculus humanus Human body louse  Phum\PHUM247280   Tribolium castaneum Red flour beetle  Tcas\GLEAN_07346
Orthologs in non-Insect Arthropods (EOG680GCC)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Ixodes scapularis Deer tick  Isca\ISCW008869
Orthologs in non-Arthropod Metazoa (EOG67X1GJ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\tpi-1   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_027281gn   Danio rerio Zebrafish  Drer\tpi1b   Danio rerio Zebrafish  Drer\tpi1a   Xenopus tropicalis Western clawed frog  Xtro\tpi1   Gallus gallus Domestic chicken  Ggal\TPI1   Mus musculus House mouse  Mmus\ENSMUSG00000023456   Rattus norvegicus Norway rat  Rnor\Tpi1   Homo sapiens Human  Hsap\TPI1
Human Orthologs (1)
Gene OMIM HGNC Hsap\TPI1
AAA Orthologs (0) based on analysis using Dmel annotation version 4.3
No orthologs identified
Stocks & Reagents
Stocks Listed in FlyBase ( 10 )
Bloomington	26304 y1 v1; P{TRiP.JF02074}attP2 16563 y1 w67c23; P{EPgy2}TpiEY03361 20699 y1 w67c23; P{EPgy2}TpiEY11806 4016 Est-6S α-Est5F FdhS Lap-DF Acph-1A Tpi6/TM6, Est-6F α-Est5S FdhF Lap-DS Acph-1B Tpi4
Kyoto	125067 w1118; P{RS5}Tpi5-HA-1094 105052 y* w*; P{GawB}TpiNP5917 / TM6, P{UAS-lacZ.UW23-1}UW23-1 112643 y* w*; P{GawB}TpiNP1356 / TM6, P{UAS-lacZ.UW23-1}UW23-1 107556 Est-6S α-Est5F FdhS Lap-DF Acph-1A Tpi6/TM6, Est-6F α-Est5S FdhF Lap-DS Acph-1B Tpi4
VDRC	v25643 w1118; P{GD10138}v25643 v25644 w1118; P{GD10138}v25644
Genomic Clones ( 1 )
	BACR05L15 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 132 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	AT02695 GH10864 GH11014
Other clones	DPiM_GH10864 DPiM_GH11014 yak-em_Tpi
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT02430 AT03179 AT03458 AT04029 AT05210 AT06829 AT08392 AT09740 AT13287 AT14336 AT14825 AT18441 AT27516 AT27672 AT28018 AT28067 AT29637 AT30024 AT31037 AT31074 GH02005 GH06796 GH12364 GH13877 GH18102 GH21177 GH21434 GM05465 GM08315 GM09220 GM22883 GM24790 GM29075 HL02126 LD01652 LD07955 LD09514 LP01167 LP04150 LP04455 LP04550 LP07015 LP08014 LP08577 LP14379 LP16525 LP18658 RE06464 RE06770 RE07242 RE08811 RE19147 RE21728 RE25141 RE26847 RE36156 RE36744 RE37855 RE45236 RE49292 RE51192 RE63767 RE65301 RE65615 RH13283 RH21561 RH21756 RH25405 RH27448 RH27784 RH29365 RH31333 RH34005 RH35538 RH36188 RH37651 RH39943 RH41787 RH43573 RH50388 RH56833 RH56835 RH57514 RH59509 RH62050 RH71777 SD17060 SD18465 SD22408 SD22779 SD24511 SD26073
Other clones	54398 57675 119523 176088 bs32h11 bs40f07 bs44c04 bs49e12 bs54a08 bs58g11 CK01.105A.E4 CK01.105A.G4 CK01.168C.E12 Dmel_dig_000463_0652_0367 Dmel_dig_000666_0640_0336 Dmel_dig_020379_0725_0322 EC40421 EK087950 EK087958 EK103450 EK147109 EK178838 EK179232 EK276542 ESG0111d.B21_E10 GM03866 IP10871 IP10971 IP11071 IP11171 IP18152 IP18515 RE01526 RH38494
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0086355 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43582
Antibody Information
	polyclonal (Sullivan et al., 2003)
Other Information
Discoverer
Etymology
	The gene has been named "sugarkill", due to the well characterised function of the Tpi protein in carbohydrate metabolism and the reduced longevity defect of Tpi mutants. (Celotto et al., 2006)
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Tpi CG2171 (Ye, 1999.11)
Source for database merge of	Source for merge of: Tpi ND14 (Celotto et al., 2006)
Additional comments
Other Comments
	Mutants have reduced longevity, progressive locomotor deficiency and neural degeneration. (Celotto et al., 2006) In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes. (Kliman and Eyre-Walker, 1998) Identified in 2D gels of CMW W2 wing imaginal disc cell proteins. (Santaren et al., 1993) Isolated using a fragment of the chicken Tpi gene as a probe. (Shaw-Lee et al., 1991) Tpi has been cloned and sequenced, and its developmental expression pattern analysed. (Shaw-Lee et al., 1991) Structural gene for the glycolytic enzyme triosephosphate isomerase (TPI) found in muscle. Heterozygote contains the parental isozymes plus a hybrid isozyme.
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_176587 NM_176588 NM_176589 RefSeq (Proteins) • NP_788764 NP_788765 NP_788766 DDBJ / EMBL / GenBank • AAC39041 AAC39042 AAC39043 AAC39044 AAC39045 AAC39046 AAC39047 AAC39048 AAC39049 AAC39050 AAC39051 AAC39052 AAC39053 AAC39054 AAC39055 AAC39056 AAC39057 AAC39058 AAC39059 AAC39060 AAC39061 AAC39062 AAC39063 AAC39064 AAC39065 AAF57011 AAN14218 AAN14219 AI114163 AI402234 BE662241 BF494254 BI634441 CAA40804 CL527709 CL868516 U60836 U60837 U60838 U60839 U60840 U60841 U60842 U60843 U60844 U60845 U60846 U60847 U60848 U60849 U60850 U60851 U60852 U60853 U60854 U60855 U60856 U60857 U60858 U60859 U60860 X57576 Entrez Gene - A searchable database of RefSeq genes. • 43582 UniProtKB/Swiss-Prot • P29613
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Triosephosphate isomerase (IPR000652) Aldolase-type TIM barrel (IPR013785) Triosephosphate isomerase, active site (IPR020861) Triosephosphate isomerase, bacterial/eukaryotic (IPR022896) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • GH10864
Linkouts
	BioGRID - A database of protein and genetic interactions • 68438 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0086355 DroID - A comprehensive database of gene and protein interactions. • FBgn0086355 DRSC - Results from RNAi screens. • FBgn0086355 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0086355 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG2171-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0086355 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43582 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43582 modMine - Data generated by the modENCODE project. • FBgn0086355
Synonyms & Secondary IDs ( 15 )
Reported As
Symbol Synonym	CG2171 (Lim et al., 2011, Perkins et al., 2009.3.31, Ambrus et al., 2009, Nair et al., 2007, Cammarato et al., 2011) ND14 (Celotto et al., 2006, ) sgk (Celotto et al., 2006) TPI (Seigle et al., 2008, Eanes, 1994, Palladino et al., 2008, Zhou et al., 2008, Sullivan et al., 2003, Hrizo and Palladino, 2010) tpi (Celotto et al., 2006) Tpi (Palladino et al., 2007, Gnerer et al., 2006, Flowers et al., 2007, Lindsley and Zimm, 1992, Gan et al., 2010, Dorus et al., 2006, Eanes et al., 2006, Tennessen et al., 2011, Japanese National Institute of Genetics, 2012.5.21) TPIS (Mushegian et al., 1998) wstd (Gnerer et al., 2006)
Name Synonym	Neurodegeneration 14 (Palladino et al., 2002) sugarkill (Celotto et al., 2006) Tpi (Cammarato et al., 2011) Triose phosphate isomerase (Seigle et al., 2008, Manh et al., 2005, Lim et al., 2011, Nair et al., 2007) Triosephosphate isomerase (Lindsley and Zimm, 1992, Bozoky et al., 2009) triose phosphate isomerase (Zhou et al., 2008, Sullivan et al., 2003, Tennessen et al., 2011) triosephosphate isomerase (Hrizo and Palladino, 2010) wasted away (Gnerer et al., 2006)
Secondary FlyBase IDs
	FBgn0003738 FBgn0063906
References ( 74 )
Generate a list of	All references relating to this gene ( 74 )
List References by type	Research paper  ( 46 ) Supplementary material  ( 8 ) Personal communication to FlyBase  ( 8 ) Abstract  ( 2 ) FlyBase analysis  ( 2 ) Book  ( 1 ) Stock list  ( 1 ) Curated genome annotation  ( 2 ) Protein sequence record  ( 1 ) Website  ( 1 ) Letter  ( 1 )
Recent research papers ( 2 )
	Lim et al., 2011, FEBS Lett. 585(19): 3079--3085 The endogenous siRNA pathway in Drosophila impacts stress resistance and lifespan by regulating metabolic homeostasis. [FBrf0216303] Tennessen et al., 2011, Cell Metab. 13(2): 139--148 The Drosophila Estrogen-Related Receptor Directs a Metabolic Switch that Supports Developmental Growth. [FBrf0212927] Show all research papers ...