Gene Dmel\tum

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General Information

Symbol

Dmel\tum

Species

D. melanogaster

Name

tumbleweed

Annotation symbol

CG13345

Feature type

protein_coding_gene

FlyBase ID

FBgn0086356

Created / Updated

2007-06-14/2007-06-14

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

49C-50C6

Sequence location

2R:9,743,648..9,746,222 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene tumbleweed is referred to in FlyBase by the symbol tum (CG13345, FBgn0086356). It has the cytological map location 49C-50C6. Its sequence location is 2R:9743648..9746222. Its molecular function is described as: Rho GTPase activator activity; GTPase activator activity; diacylglycerol binding; signal transducer activity. It is involved in the biological processes: dendrite morphogenesis; neuron development; Rho protein signal transduction; cell proliferation; negative regulation of Wnt receptor signaling pathway; cytokinesis; mitotic spindle organization and biogenesis. 27 alleles are reported. The phenotypes of these alleles are annotated with 18 unique terms, many of which group under: adult segment; organ system; nervous system; adult brain; peripheral nervous system; adult mesothoracic segment; thoracic segment; cuticle; contractile ring; wing hair; cell cycle; dorsal thoracic disc. It has one annotated transcript and one annotated polypeptide.

External Summaries

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

49C-50C6

Left limit from (method unavailable) (FBrf0112136) Right limit from (method unavailable) (FBrf0112136); Limits computationally determined from genome sequence between P{lacW}l(2)03105^k16702&P{lacW}fl(2)d^k16105 and P{lacW}shot^k03010

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

50C4-50C8

(determined by in situ hybridisation)

(Sotillos and Campuzano, 2000)

50C4-50C6

(Sotillos, 1999.12.6)

49C-50D

(Gao et al., 1999)

49C-50D

(Gao, 1999.11.4)

44F-45E

Determined by deficiency mapping (details unspecified).

(Gao et al., 1999)

Experimentally Determined Recombination Data

Location

Left of (cM)

(Jones, 2005)

Right of (cM)

(Jones, 2005)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\tum for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

tum-RA

FBtr0087641

NM_137068

2388

625

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

tum-PA

FBpp0086767

69.8

625

9.33

NP_610912

AAF58324

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Rho GTPase activation protein (IPR008936)

Protein kinase C, phorbol ester/diacylglycerol binding (IPR002219)

RhoGAP (IPR000198)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Maps to

DS04160

(Sotillos and Campuzano, 2000)

Does NOT map to

Identified with

RE37229

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

point mutation

RacGAP50C[tum347]
2R:9,745,588..9,745,588

evidence=experimental
na_change=G9745588T
pr_change=E114@|RacGAP50C-PA
reported_na_change=GAG?TAG
reported_pr_change=E114@

(Goldstein, 2005)

point mutation

RacGAP50C[DH15]
2R:9,745,345..9,745,345

comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
evidence=experimental
na_change=C9745345T
pr_change=R195@|RacGAP50C-PA
reported_pr_change=R195@

(Jones, 2005)

point mutation

RacGAP50C[tum1]
2R:9,745,087..9,745,087

evidence=experimental
na_change=C9745087T
pr_change=R281@|RacGAP50C-PA
reported_na_change=CGA?TGA
reported_pr_change=R281@

(Goldstein, 2005)

point mutation

RacGAP50C[AR2]
2R:9,744,449..9,744,450

comment=G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation. (exact site of mutation unspecified). Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
evidence=experimental
pr_change=W470@|RacGAP50C-PA
reported_pr_change=W470@

(Jones, 2005)

External Data

Linkouts

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

C | S2 cells

(Somers and Saint, 2003)

embryonic stage | embryonic cycle 16

(Somers and Saint, 2003)

embryonic stage | embryonic cycle 16

(Somers and Saint, 2003)

C | S2 cells

(Somers and Saint, 2003)

embryonic stage | embryonic cycle 16

(Somers and Saint, 2003)

C | S2 cells

(Somers and Saint, 2003)

C | S2 cells

(Somers and Saint, 2003)

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal | embryonic stage (with tum^DH15)

tum^AR2

lethal | embryonic stage (with tum^AR2)

tum^DH15

lethal | embryonic stage | recessive

lethal, with Scer\GAL4^αTub84B.PL

tum^{dsRNA.Scer\UAS.cBa}

semi-lethal, with Scer\GAL4^T80

tum^{dsRNA.Scer\UAS.cBa}

Other Phenotypes

Allele

visible, with Scer\GAL4^69B

visible, with Scer\GAL4^Bx-MS1096

visible, with Scer\GAL4^en-e16E

visible, with Scer\GAL4^ey.PB

tum^{dsRNA.Scer\UAS.cBa}

increased cell size | pupal stage, with Scer\GAL4^en-e16E

tum^{dsRNA.Scer\UAS.cSa}

cell death increase, with Scer\GAL4^en-e16E

tum^{ΔE1E.Scer\UAS}

mitotic cell cycle defective

mitotic cell cycle defective | pupal stage, with Scer\GAL4^en-e16E

tum^{dsRNA.Scer\UAS.cSa}

mitotic cell cycle defective, with Scer\GAL4^en-e16E

tum^{ΔE1E.Scer\UAS}

cytokinesis defective

tum^{dsRNA.1110-1961}

cytokinesis defective | somatic clone

tum³⁴⁷

cytokinesis defective, with Scer\GAL4^OK107

tum^{dsRNA.Scer\UAS}

neuroanatomy defective, with Scer\GAL4^OK107

tum^{dsRNA.Scer\UAS.cBa}

tissue polarity defective, with Scer\GAL4^69B

tissue polarity defective, with Scer\GAL4^Bx-MS1096

tissue polarity defective, with Scer\GAL4^en-e16E

Phenotype manifest in

Allele

dendrite & embryo | dorsal

tum¹⁴

muscle cell & embryo

tum¹⁴

embryonic epidermis

tum¹⁴

neuron & embryo

tum¹⁴

embryonic central nervous system & neuron

tum¹⁴

axon & embryo

tum¹⁴

mushroom body & axon | somatic clone

tum³⁴⁷

alpha'-lobe

tum³⁴⁷

beta'-lobe

tum³⁴⁷

alpha-lobe

tum³⁴⁷

beta-lobe

tum³⁴⁷

mushroom body & axon, with Scer\GAL4^OK107

wing, with Scer\GAL4^en-e16E

wing vein, with Scer\GAL4^en-e16E

dorsal mesothoracic disc, with Scer\GAL4^en-e16E

tum^{ΔE1E.Scer\UAS}

sensillum campaniformium & wing vein L3 | supernumerary, with Scer\GAL4^en-e16E

sensillum campaniformium & wing vein L4 | supernumerary, with Scer\GAL4^en-e16E

sensillum campaniformium & wing vein L5 | supernumerary, with Scer\GAL4^en-e16E

wing & cell, with Scer\GAL4^en-e16E

wing & trichome, with Scer\GAL4^en-e16E

wing, with Scer\GAL4^Bx-MS1096

wing vein, with Scer\GAL4^Bx-MS1096

sensillum campaniformium & wing vein L3 | supernumerary, with Scer\GAL4^Bx-MS1096

sensillum campaniformium & wing vein L4 | supernumerary, with Scer\GAL4^Bx-MS1096

sensillum campaniformium & wing vein L5 | supernumerary, with Scer\GAL4^Bx-MS1096

wing & cell, with Scer\GAL4^Bx-MS1096

wing & trichome, with Scer\GAL4^Bx-MS1096

wing, with Scer\GAL4^69B

wing vein, with Scer\GAL4^69B

sensillum campaniformium & wing vein L3 | supernumerary, with Scer\GAL4^69B

sensillum campaniformium & wing vein L4 | supernumerary, with Scer\GAL4^69B

sensillum campaniformium & wing vein L5 | supernumerary, with Scer\GAL4^69B

wing & cell, with Scer\GAL4^69B

wing & trichome, with Scer\GAL4^69B

denticle

embryonic/first instar larval cuticle

denticle row

embryonic epidermis & nucleus

tum^AR2

wing vein, with Scer\GAL4^C-765

tum^Scer\UAS.cSa

wing, with Scer\GAL4^C-765

tum^Scer\UAS.cSa

sensillum campaniformium, with Scer\GAL4^en-e16E

tum^Scer\UAS.cSa

mitotic telophase

tum^{dsRNA.1110-1961}

mitotic telophase & spindle

tum^{dsRNA.1110-1961}

contractile ring

tum^{dsRNA.1110-1961}

eye, with Scer\GAL4^ey.PB

tum^{dsRNA.Scer\UAS.cBa}

ommatidium, with Scer\GAL4^ey.PB

tum^{dsRNA.Scer\UAS.cBa}

eye & ommatidium, with Scer\GAL4^ey.PB

tum^{dsRNA.Scer\UAS.cBa}

mushroom body, with Scer\GAL4^OK107

tum^{dsRNA.Scer\UAS.cBa}

wing hair | posterior compartment, with Scer\GAL4^en-e16E

tum^{dsRNA.Scer\UAS.cSa}

S2 cell-line & cell

tum^dsRNA.cHa

S2 cell-line & spindle microtubule

tum^dsRNA.cHa

Classical Alleles ( 8 )

For All Classical Alleles Show

Allele of tum	Class	Mutagen	Stocks	Known lesion
tum¹⁴		ethyl methanesulfonate	0	--
tum¹			0	Yes
tum³⁴⁷			0	Yes
tum^AR2	loss of function	ethyl methanesulfonate	1	Yes
tum^DH15	loss of function	ethyl methanesulfonate	1	Yes
tum^EY01491b		P-element activity	1	--
tum^{dsRNA.H.Scer\UAS}			0	--
tum^unspecified			0	--

Alleles Carried on Transgenic Constructs ( 19 )

For All Alleles Carried on Transgenic Constructs Show

Allele of tum	Mutagen	Stocks	Known lesion
tum^GD7990	in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
tum^{P417Q.Scer\UAS}	in vitro construct \| regulatory fusion in vitro construct \| amino acid replacement	0	Yes
tum^{R417L.Scer\UAS.T:Hsap\MYC,T:Avic\GFP-EYFP}	in vitro construct \| site dire