• Home
• Tools
  • Tools Overview
  • Query Tools

    
    • QuickSearch
    • QueryBuilder
    • TermLink
    • CytoSearch
    • Interactions Browser
    • ImageBrowse
    • Find A Person
    • Google™ FlyBase
  • Genomic/Map Tools

    
    • BLAST
    • GBrowse
    • CytoSearch
    • Aberrations Maps
    • Chromosome Maps
    • Coordinates Converter
  • Retrieve/Convert

    
    • Batch Download
    • Coordinates Converter
    • ID Converter
  • Data Submission

    
    • Fast-Track Your Paper
    • Submit Personal Comm
    • Add A New Person
    • Update Person Info
  • People

    
    • Find A Person
    • Add A New Person
    • Update Person Info
• Files
  • Files Overview
  • Downloads:
  • Precomputed files
  • Archived data
  • Releases (FTP)
  • Genomes (FTP)
  • AAA data (FTP)
  • Lists:
  • Transposons (Dmel)
  • Transposons (other)
  • Vectors & Constructs
  • Anatomical Terms
  • Map Conversion
• Species
  • Phylogeny
  • Sequenced Species
  • Synteny Table
  • Color Illustrations
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All Resources
  • Model Organisms
  • Stock Collections
  • Other links:
  • BDGP
  • DGRC
  • FlyExpress
  • Interactive Fly
  • modENCODE
  • Textpresso for Fly
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • FlyBase Guides
  • Video Tutorials
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Contact FlyBase
• Archives
  • Prior Release
  • Other Archives
  • Coordinate Converter

Gene Dmel\tum

General Information
Symbol	Dmel\tum	Species	D. melanogaster
Name	tumbleweed	Annotation symbol	CG13345
Feature type	protein_coding_gene	FlyBase ID	FBgn0086356
Gene Model Status	Current	Stock availability	12 publicly available
Also Known As	racGAP50C, AcGAP, RacGAP, DRacGAP
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	49C-50C6	Sequence location	2R:9,743,655..9,746,222 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene tumbleweed is referred to in FlyBase by the symbol Dmel\tum (CG13345, FBgn0086356). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: signal transducer activity; diacylglycerol binding; GTPase activator activity. There is experimental evidence for 12 unique biological process terms, many of which group under: biological regulation; cellular component organization or biogenesis; cellular process; system development; neuron differentiation; cell cycle; regulation of Wnt receptor signaling pathway; cell cycle process; cell proliferation; cell fate commitment. 40 alleles are reported. The phenotypes of these alleles are annotated with: organ system; organ system subdivision; adult segment; external compound sense organ; thoracic segment; acellular anatomical structure; organelle; adult; cellular component organization or biogenesis; cytoplasmic part; nucleus. It has one annotated transcript and one annotated polypeptide. Protein features are: Protein kinase C-like, phorbol ester/diacylglycerol binding; Rho GTPase activation protein; Rho GTPase-activating protein domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1634690_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of tum. Gene sequence location is 2R:9743655..9746222.
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2012_01	References Müller et al., 2010, EMBO J. 29(19): 3344--3357 Controlled Vocabulary Terms centrosome duplication Sequence features BKN29362 HFA07575
FB2011_10	References Bakal et al., 2007, Science 316(5832): 1753--1756 Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593 Transgenic Constructs P{UAS-Venus-tum}
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 49C-50C6   Left limit from (method unavailable) (FBrf0112136) Right limit from (method unavailable) (FBrf0112136); Limits computationally determined from genome sequence between P{lacW}l(2)03105k16702&P{lacW}fl(2)dk16105 and P{lacW}shotk03010
Experimentally Determined Cytological Location
Cytogenetic map Notes References 44F-45E   Determined by deficiency mapping (details unspecified).   (Gao et al., 1999) 50C4-50C8   (determined by in situ hybridisation)   (Sotillos and Campuzano, 2000) 50C4-50C6     (Sotillos, 1999.12.6) 49C-50D     (Gao et al., 1999) 49C-50D     (Gao, 1999.11.4)
Experimentally Determined Recombination Data
Location
Left of (cM)	P{lacW}shotk03010 c (Jones, 2005)
Right of (cM)	cn P{EP}EP2423 (Jones, 2005)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\tum for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) tum-RA FBtr0087641  NM_137068   2381   625
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein tum-PA   FBpp0086767   69.8   625   9.33     NP_610912   AAF58324
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Rho GTPase activation protein (IPR008936) Protein kinase C-like, phorbol ester/diacylglycerol binding (IPR002219) Rho GTPase-activating protein domain (IPR000198)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA202212   AA202642   AA246503   AA263282   AA264130   AA264948   AA390664   AA391210   AA391238   AA391836   AA439580   AA439929   AA538613   AA538658   AA538744   AA539253   AA540080   AA695469   AA735254   AA816838   AA817070   AA820454   AA941001   AA941099   AA941482   AA949496   AA950110   AA950644   AA950649   AA978566   AE013599 AAF58324   AC005977   AI514640   AI516108   AI518434   AI520206   AI532592   AI532889   AI543424   AI544299   AI546733   AJ251502 CAB96203   AY119631 AAM50285   BF503992   BF505729   BG635833   BG638436   BI162916   BI167108   BI167373   BI168555   BI173766   BI212583   BI216838   BI232527   BI240389   BI243502   BI354352   BI354401   BI356231   BI357731   BI362177   BI365342   BI367880   BI374619   BI481612   BI484323   BI485929   BI486380   BI578903   BI579502   BI579734   BI579914   BI632902   BI633103   BI633877   BI638377   BI638434   BI639592   BI640110   BI640691   BI640699   BI641110   BI641179   BP559850   BZ665555   CO263794   CO302772   CO303808   CO310226   CO312412   CO318853   CO319474   CO320962   CO326393   CO327024   CO333962   EC060789   EC078394   EC083810   EC085915   EC090152   EC205440   EC225087   EC227333   EC246010   EC250245   EC251901   EC253084   EV578196   GH738830   GH739597   GH741150   GH741292   GH744436   GH745579   GH746234   GH747553   GH747565   GH761703   GH763039   GH764941   GH767288   GH767677   GH769922   GH769959   GH779357   GH779714   GH779781   GO254357
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9N9Z9 Q8MRH0 A1Z9I5
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic cycle 16   organism   • midbody (Somers and Saint, 2003) Comment:telophase organism   • spindle & cell cortex | mid (Somers and Saint, 2003) Comment:anaphase organism   • spindle (Somers and Saint, 2003) Comment:metaphase   cell   • midbody (Somers and Saint, 2003) Comment:telophase; expression assayed in cultured S2 cells cell   • nucleus (Somers and Saint, 2003) Comment:interphase; expression assayed in cultured S2 cells cell   • spindle & cell cortex | mid (Somers and Saint, 2003) Comment:expression assayed in cultured S2 cells; anaphase cell   • spindle (Somers and Saint, 2003) Comment:metaphase; expression assayed in cultured S2 cells
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0086356    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0086356 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed within 00-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0086356 expression level Developmental Stage   Expression Level embryo 00-02hr    12744 embryo 02-04hr    8552 embryo 04-06hr    11141 embryo 06-08hr    12049 embryo 08-10hr    9228 embryo 10-12hr    5180 embryo 12-14hr    2975 embryo 14-16hr    3797 embryo 16-18hr    2640 embryo 18-20hr    1325 embryo 20-22hr    3785 embryo 22-24hr    3059 larva L1    129 larva L2    225 larva L3 12hr old    341 larva L3 puffstage 1-2    844 larva L3 puffstage 3-6    1115 larva L3 puffstage 7-9    1096 white prepupae new    889 white prepupae 12hr    2424 white prepupae 24hr    1549 pupae 2d postWPP    376 pupae 3d postWPP    209 pupae 4d postWPP    394 adult male 01day    206 adult male 05day    313 adult male 30day    345 adult female 01day    2568 adult female 05day    4967 adult female 30day    5383 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (12744) embryo 02-04hr  (8552) embryo 04-06hr  (11141) embryo 06-08hr  (12049) embryo 08-10hr  (9228) embryo 10-12hr  (5180) embryo 12-14hr  (2975) embryo 14-16hr  (3797) embryo 16-18hr  (2640) embryo 18-20hr  (1325) embryo 20-22hr  (3785) embryo 22-24hr  (3059) larva L1  (129) larva L2  (225) larva L3 12hr old  (341) larva L3 puffstage 1-2  (844) larva L3 puffstage 3-6  (1115) larva L3 puffstage 7-9  (1096) white prepupae new  (889) white prepupae 12hr  (2424) white prepupae 24hr  (1549) pupae 2d postWPP  (376) pupae 3d postWPP  (209) pupae 4d postWPP  (394) adult male 01day  (206) adult male 05day  (313) adult male 30day  (345) adult female 01day  (2568) adult female 05day  (4967) adult female 30day  (5383) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (12744) embryo 02-04hr  (8552) embryo 04-06hr  (11141) embryo 06-08hr  (12049) embryo 08-10hr  (9228) embryo 10-12hr  (5180) embryo 12-14hr  (2975) embryo 14-16hr  (3797) embryo 16-18hr  (2640) embryo 18-20hr    1325 embryo 20-22hr  (3785) embryo 22-24hr  (3059) larva L1    129 larva L2    225 larva L3 12hr old    341 larva L3 puffstage 1-2    844 larva L3 puffstage 3-6    1115 larva L3 puffstage 7-9    1096 white prepupae new    889 white prepupae 12hr  (2424) white prepupae 24hr  (1549) pupae 2d postWPP    376 pupae 3d postWPP    209 pupae 4d postWPP    394 adult male 01day    206 adult male 05day    313 adult male 30day    345 adult female 01day  (2568) adult female 05day  (4967) adult female 30day  (5383) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (12744) embryo 02-04hr    8552 embryo 04-06hr  (11141) embryo 06-08hr  (12049) embryo 08-10hr    9228 embryo 10-12hr    5180 embryo 12-14hr    2975 embryo 14-16hr    3797 embryo 16-18hr    2640 embryo 18-20hr    1325 embryo 20-22hr    3785 embryo 22-24hr    3059 larva L1    129 larva L2    225 larva L3 12hr old    341 larva L3 puffstage 1-2    844 larva L3 puffstage 3-6    1115 larva L3 puffstage 7-9    1096 white prepupae new    889 white prepupae 12hr    2424 white prepupae 24hr    1549 pupae 2d postWPP    376 pupae 3d postWPP    209 pupae 4d postWPP    394 adult male 01day    206 adult male 05day    313 adult male 30day    345 adult female 01day    2568 adult female 05day    4967 adult female 30day    5383 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    12744 embryo 02-04hr    8552 embryo 04-06hr    11141 embryo 06-08hr    12049 embryo 08-10hr    9228 embryo 10-12hr    5180 embryo 12-14hr    2975 embryo 14-16hr    3797 embryo 16-18hr    2640 embryo 18-20hr    1325 embryo 20-22hr    3785 embryo 22-24hr    3059 larva L1    129 larva L2    225 larva L3 12hr old    341 larva L3 puffstage 1-2    844 larva L3 puffstage 3-6    1115 larva L3 puffstage 7-9    1096 white prepupae new    889 white prepupae 12hr    2424 white prepupae 24hr    1549 pupae 2d postWPP    376 pupae 3d postWPP    209 pupae 4d postWPP    394 adult male 01day    206 adult male 05day    313 adult male 30day    345 adult female 01day    2568 adult female 05day    4967 adult female 30day    5383 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0086356 expression level Developmental Stage   Expression Level embryo 00-02hr    12744 embryo 02-04hr    8552 embryo 04-06hr    11141 embryo 06-08hr    12049 embryo 08-10hr    9228 embryo 10-12hr    5180 embryo 12-14hr    2975 embryo 14-16hr    3797 embryo 16-18hr    2640 embryo 18-20hr    1325 embryo 20-22hr    3785 embryo 22-24hr    3059 larva L1    129 larva L2    225 larva L3 12hr old    341 larva L3 puffstage 1-2    844 larva L3 puffstage 3-6    1115 larva L3 puffstage 7-9    1096 white prepupae new    889 white prepupae 12hr    2424 white prepupae 24hr    1549 pupae 2d postWPP    376 pupae 3d postWPP    209 pupae 4d postWPP    394 adult male 01day    206 adult male 05day    313 adult male 30day    345 adult female 01day    2568 adult female 05day    4967 adult female 30day    5383 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (12744) embryo 02-04hr  (8552) embryo 04-06hr  (11141) embryo 06-08hr  (12049) embryo 08-10hr  (9228) embryo 10-12hr  (5180) embryo 12-14hr  (2975) embryo 14-16hr  (3797) embryo 16-18hr  (2640) embryo 18-20hr  (1325) embryo 20-22hr  (3785) embryo 22-24hr  (3059) larva L1  129 larva L2  (225) larva L3 12hr old  (341) larva L3 puffstage 1-2  (844) larva L3 puffstage 3-6  (1115) larva L3 puffstage 7-9  (1096) white prepupae new  (889) white prepupae 12hr  (2424) white prepupae 24hr  (1549) pupae 2d postWPP  (376) pupae 3d postWPP  (209) pupae 4d postWPP  (394) adult male 01day  (206) adult male 05day  (313) adult male 30day  (345) adult female 01day  (2568) adult female 05day  (4967) adult female 30day  (5383) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (12744) embryo 02-04hr  (8552) embryo 04-06hr  (11141) embryo 06-08hr  (12049) embryo 08-10hr  (9228) embryo 10-12hr  (5180) embryo 12-14hr  (2975) embryo 14-16hr  (3797) embryo 16-18hr  (2640) embryo 18-20hr    1325 embryo 20-22hr  (3785) embryo 22-24hr  (3059) larva L1    129 larva L2    225 larva L3 12hr old    341 larva L3 puffstage 1-2    844 larva L3 puffstage 3-6    1115 larva L3 puffstage 7-9    1096 white prepupae new    889 white prepupae 12hr  2424 white prepupae 24hr  1549 pupae 2d postWPP    376 pupae 3d postWPP    209 pupae 4d postWPP    394 adult male 01day    206 adult male 05day    313 adult male 30day    345 adult female 01day  (2568) adult female 05day  (4967) adult female 30day  (5383) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  12744 embryo 02-04hr    8552 embryo 04-06hr  11141 embryo 06-08hr  12049 embryo 08-10hr    9228 embryo 10-12hr    5180 embryo 12-14hr    2975 embryo 14-16hr    3797 embryo 16-18hr    2640 embryo 18-20hr    1325 embryo 20-22hr    3785 embryo 22-24hr    3059 larva L1    129 larva L2    225 larva L3 12hr old    341 larva L3 puffstage 1-2    844 larva L3 puffstage 3-6    1115 larva L3 puffstage 7-9    1096 white prepupae new    889 white prepupae 12hr    2424 white prepupae 24hr    1549 pupae 2d postWPP    376 pupae 3d postWPP    209 pupae 4d postWPP    394 adult male 01day    206 adult male 05day    313 adult male 30day    345 adult female 01day    2568 adult female 05day    4967 adult female 30day    5383 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    12744 embryo 02-04hr    8552 embryo 04-06hr    11141 embryo 06-08hr    12049 embryo 08-10hr    9228 embryo 10-12hr    5180 embryo 12-14hr    2975 embryo 14-16hr    3797 embryo 16-18hr    2640 embryo 18-20hr    1325 embryo 20-22hr    3785 embryo 22-24hr    3059 larva L1    129 larva L2    225 larva L3 12hr old    341 larva L3 puffstage 1-2    844 larva L3 puffstage 3-6    1115 larva L3 puffstage 7-9    1096 white prepupae new    889 white prepupae 12hr    2424 white prepupae 24hr    1549 pupae 2d postWPP    376 pupae 3d postWPP    209 pupae 4d postWPP    394 adult male 01day    206 adult male 05day    313 adult male 30day    345 adult female 01day    2568 adult female 05day    4967 adult female 30day    5383 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0086356    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0086356 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0086356 expression level Tissue   Expression Level Larval Central Nervous System    296.55 Larval Midgut no informative data Larval Hindgut    19.8 Larval Malpighian Tubules    6.5 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    52.975 Larval Carcass no informative data Adult Head    6.6 Adult Eye    3.275 Adult Brain    5 Adult Thoracic-Abdominal Ganglion    4.1 Adult Crop    8.7 Adult Midgut no informative data Adult Hindgut    6.7 Adult Malpighian Tubules    7.5 Adult Fat Body    3.1 Adult Salivary Gland no informative data Adult Heart    2.3 Adult VirginFemale Spermatheca    7.2 Adult InseminatedFemale Spermatheca    6.3 Adult Ovary    445.4 Adult Testis    82.1 Adult Male Accessory Gland    4.3 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    296.55 Larval Midgut no informative data Larval Hindgut    19.8 Larval Malpighian Tubules    6.5 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    52.975 Larval Carcass no informative data Adult Head    6.6 Adult Eye    3.275 Adult Brain    5 Adult Thoracic-Abdominal Ganglion    4.1 Adult Crop    8.7 Adult Midgut no informative data Adult Hindgut    6.7 Adult Malpighian Tubules    7.5 Adult Fat Body    3.1 Adult Salivary Gland no informative data Adult Heart    2.3 Adult VirginFemale Spermatheca    7.2 Adult InseminatedFemale Spermatheca    6.3 Adult Ovary    445.4 Adult Testis    82.1 Adult Male Accessory Gland    4.3 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    296.55 Larval Midgut no informative data Larval Hindgut    19.8 Larval Malpighian Tubules    6.5 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    52.975 Larval Carcass no informative data Adult Head    6.6 Adult Eye    3.275 Adult Brain    5 Adult Thoracic-Abdominal Ganglion    4.1 Adult Crop    8.7 Adult Midgut no informative data Adult Hindgut    6.7 Adult Malpighian Tubules    7.5 Adult Fat Body    3.1 Adult Salivary Gland no informative data Adult Heart    2.3 Adult VirginFemale Spermatheca    7.2 Adult InseminatedFemale Spermatheca    6.3 Adult Ovary    445.4 Adult Testis    82.1 Adult Male Accessory Gland    4.3 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    296.55 Larval Midgut no informative data Larval Hindgut    19.8 Larval Malpighian Tubules    6.5 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    52.975 Larval Carcass no informative data Adult Head    6.6 Adult Eye    3.275 Adult Brain    5 Adult Thoracic-Abdominal Ganglion    4.1 Adult Crop    8.7 Adult Midgut no informative data Adult Hindgut    6.7 Adult Malpighian Tubules    7.5 Adult Fat Body    3.1 Adult Salivary Gland no informative data Adult Heart    2.3 Adult VirginFemale Spermatheca    7.2 Adult InseminatedFemale Spermatheca    6.3 Adult Ovary    445.4 Adult Testis    82.1 Adult Male Accessory Gland    4.3 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0086356 expression level Tissue   Expression Level Larval Central Nervous System    296.55 Larval Midgut no informative data Larval Hindgut    19.8 Larval Malpighian Tubules    6.5 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    52.975 Larval Carcass no informative data Adult Head    6.6 Adult Eye    3.275 Adult Brain    5 Adult Thoracic-Abdominal Ganglion    4.1 Adult Crop    8.7 Adult Midgut no informative data Adult Hindgut    6.7 Adult Malpighian Tubules    7.5 Adult Fat Body    3.1 Adult Salivary Gland no informative data Adult Heart    2.3 Adult VirginFemale Spermatheca    7.2 Adult InseminatedFemale Spermatheca    6.3 Adult Ovary    445.4 Adult Testis    82.1 Adult Male Accessory Gland    4.3 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    296.55 Larval Midgut no informative data Larval Hindgut    19.8 Larval Malpighian Tubules    6.5 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    52.975 Larval Carcass no informative data Adult Head    6.6 Adult Eye    3.275 Adult Brain    5 Adult Thoracic-Abdominal Ganglion    4.1 Adult Crop    8.7 Adult Midgut no informative data Adult Hindgut    6.7 Adult Malpighian Tubules    7.5 Adult Fat Body    3.1 Adult Salivary Gland no informative data Adult Heart    2.3 Adult VirginFemale Spermatheca    7.2 Adult InseminatedFemale Spermatheca    6.3 Adult Ovary    445.4 Adult Testis    82.1 Adult Male Accessory Gland    4.3 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    296.55 Larval Midgut no informative data Larval Hindgut    19.8 Larval Malpighian Tubules    6.5 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    52.975 Larval Carcass no informative data Adult Head    6.6 Adult Eye    3.275 Adult Brain    5 Adult Thoracic-Abdominal Ganglion    4.1 Adult Crop    8.7 Adult Midgut no informative data Adult Hindgut    6.7 Adult Malpighian Tubules    7.5 Adult Fat Body    3.1 Adult Salivary Gland no informative data Adult Heart    2.3 Adult VirginFemale Spermatheca    7.2 Adult InseminatedFemale Spermatheca    6.3 Adult Ovary    445.4 Adult Testis    82.1 Adult Male Accessory Gland    4.3 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    296.55 Larval Midgut no informative data Larval Hindgut    19.8 Larval Malpighian Tubules    6.5 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    52.975 Larval Carcass no informative data Adult Head    6.6 Adult Eye    3.275 Adult Brain    5 Adult Thoracic-Abdominal Ganglion    4.1 Adult Crop    8.7 Adult Midgut no informative data Adult Hindgut    6.7 Adult Malpighian Tubules    7.5 Adult Fat Body    3.1 Adult Salivary Gland no informative data Adult Heart    2.3 Adult VirginFemale Spermatheca    7.2 Adult InseminatedFemale Spermatheca    6.3 Adult Ovary    445.4 Adult Testis    82.1 Adult Male Accessory Gland    4.3 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut no informative data Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea     Larval Carcass no informative data Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut no informative data Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland no informative data Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass no informative data FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    6.6   NA  Eye    3.275   NA  Brain    5   296.55  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    4.1   NA  Crop    8.7   no informative data  Midgut    no informative data   19.8  Hindgut    6.7   6.5  Malpighian Tubules    7.5   no informative data  Fat Body    3.1   no informative data  Salivary Gland    no informative data   NA  Heart    2.3   52.975  Trachea    NA   NA  VirginFemale Spermatheca    7.2   NA  InseminatedFemale Spermatheca    6.3   NA  Ovary    445.4   NA  Testis    82.1   NA  Male Accessory Gland    4.3   no informative data  Carcass    no informative data modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_17
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0086356 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG13345-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL41407 tumGD7990 lethal, with Scer\GAL4αTub84B.PL tumdsRNA.Scer\UAS.cBa lethal | embryonic stage (with tumAR2) tumDH15 lethal | embryonic stage (with tumDH15) tumAR2 lethal | embryonic stage | recessive tumAR2 tumDH15 semi-lethal, with Scer\GAL4T80 tumdsRNA.Scer\UAS.cBa viable, with Scer\GAL4pnr-MD237 tumGD7990 Other Phenotypes Allele cytokinesis defective tumdsRNA.1110-1961 cytokinesis defective, with Scer\GAL4ey-OK107 tumdsRNA.Scer\UAS cytokinesis defective | recessive tumDH15 cytokinesis defective | somatic clone tum347 increased cell death, with Scer\GAL4en-e16E tumΔE1E.Scer\UAS increased cell size | pupal stage, with Scer\GAL4en-e16E tumdsRNA.Scer\UAS.cSa mitotic cell cycle defective tumdsRNA.cHa tumdsRNA.cSa mitotic cell cycle defective, with Scer\GAL4en-e16E tumΔE1E.Scer\UAS mitotic cell cycle defective | pupal stage, with Scer\GAL4en-e16E tumdsRNA.Scer\UAS.cSa neuroanatomy defective, with Scer\GAL4ey-OK107 tumdsRNA.Scer\UAS.cBa neuroanatomy defective | larval stage, with Scer\GAL41407 tumGD7990 planar polarity defective, with Scer\GAL469B tumP417Q.Scer\UAS tumΔE1E.Scer\UAS planar polarity defective, with Scer\GAL4Bx-MS1096 tumP417Q.Scer\UAS tumΔE1E.Scer\UAS planar polarity defective, with Scer\GAL4en-e16E tumdsRNA.Scer\UAS.cSa tumP417Q.Scer\UAS tumΔE1E.Scer\UAS visible, with Scer\GAL469B tumP417Q.Scer\UAS tumΔE1E.Scer\UAS visible, with Scer\GAL4Bx-MS1096 tumP417Q.Scer\UAS tumΔE1E.Scer\UAS visible, with Scer\GAL4en-e16E tumJF01093 tumJF01678 tumP417Q.Scer\UAS tumΔE1E.Scer\UAS visible, with Scer\GAL4ey.PB tumdsRNA.Scer\UAS.cBa visible, with Scer\GAL4pnr-MD237 tumGD7990 Phenotype manifest in Allele abdominal ventral denticle belt | embryonic stage tumAR2 actomyosin contractile ring tumdsRNA.1110-1961 axon | embryonic stage tum14 dendrite | dorsal | embryonic stage tum14 denticle tumAR2 tumDH15 denticle row tumAR2 tumDH15 embryonic/first instar larval cuticle tumAR2 tumDH15 embryonic/larval muscle system tumDH15 embryonic/larval neuroblast | larval stage, with Scer\GAL41407 tumGD7990 embryonic central nervous system & neuron tum14 embryonic epidermis tum14 embryonic epidermis & nucleus tumAR2 epithelium | embryonic stage tumAR2 epithelium | embryonic stage (with Df(2R)Exel7128) tumAR2 epithelium | extended germ band stage tumDH15 epithelium | extended germ band stage (with Df(2R)Exel7128) tumDH15 eye, with Scer\GAL4ey.PB tumdsRNA.Scer\UAS.cBa eye & ommatidium, with Scer\GAL4ey.PB tumdsRNA.Scer\UAS.cBa ganglion mother cell | larval stage, with Scer\GAL41407 tumGD7990 mesothoracic tergum, with Scer\GAL4pnr-MD237 tumGD7990 mitotic telophase tumdsRNA.1110-1961 mitotic telophase & spindle tumdsRNA.1110-1961 muscle cell | embryonic stage tum14 mushroom body, with Scer\GAL4ey-OK107 tumdsRNA.Scer\UAS.cBa mushroom body & axon, with Scer\GAL4ey-OK107 tumdsRNA.Scer\UAS tumdsRNA.Scer\UAS.cBa mushroom body & axon | somatic clone tum347 mushroom body alpha'-lobe tum347 mushroom body alpha-lobe tum347 mushroom body beta'-lobe tum347 mushroom body beta-lobe tum347 nervous system | embryonic stage tumDH15 neuron | embryonic stage tum14 nucleus tumAR2 nucleus (with Df(2R)Exel7128) tumAR2 nucleus | extended germ band stage tumDH15 nucleus | extended germ band stage (with Df(2R)Exel7128) tumDH15 ommatidium, with Scer\GAL4ey.PB tumdsRNA.Scer\UAS.cBa S2 cell-line & cell tumdsRNA.cHa S2 cell-line & spindle microtubule tumdsRNA.cHa sensillum campaniformium, with Scer\GAL4en-e16E tumScer\UAS.cSa sensillum campaniformium & wing vein L3 | supernumerary, with Scer\GAL469B tumΔE1E.Scer\UAS tumP417Q.Scer\UAS sensillum campaniformium & wing vein L3 | supernumerary, with Scer\GAL4Bx-MS1096 tumΔE1E.Scer\UAS tumP417Q.Scer\UAS sensillum campaniformium & wing vein L3 | supernumerary, with Scer\GAL4en-e16E tumΔE1E.Scer\UAS tumP417Q.Scer\UAS sensillum campaniformium & wing vein L4 | supernumerary, with Scer\GAL469B tumΔE1E.Scer\UAS tumP417Q.Scer\UAS sensillum campaniformium & wing vein L4 | supernumerary, with Scer\GAL4Bx-MS1096 tumΔE1E.Scer\UAS tumP417Q.Scer\UAS sensillum campaniformium & wing vein L4 | supernumerary, with Scer\GAL4en-e16E tumΔE1E.Scer\UAS tumP417Q.Scer\UAS sensillum campaniformium & wing vein L5 | supernumerary, with Scer\GAL469B tumΔE1E.Scer\UAS tumP417Q.Scer\UAS sensillum campaniformium & wing vein L5 | supernumerary, with Scer\GAL4Bx-MS1096 tumΔE1E.Scer\UAS tumP417Q.Scer\UAS sensillum campaniformium & wing vein L5 | supernumerary, with Scer\GAL4en-e16E tumΔE1E.Scer\UAS tumP417Q.Scer\UAS wing, with Scer\GAL469B tumΔE1E.Scer\UAS tumP417Q.Scer\UAS wing, with Scer\GAL4ap-md544 tumScer\UAS.cSa wing, with Scer\GAL4Bx-MS1096 tumΔE1E.Scer\UAS tumP417Q.Scer\UAS tumScer\UAS.cSa wing, with Scer\GAL4C-765 tumScer\UAS.cSa wing, with Scer\GAL4en-e16E tumJF01093 tumJF01678 tumΔE1E.Scer\UAS tumP417Q.Scer\UAS tumScer\UAS.cSa wing & cell, with Scer\GAL469B tumΔE1E.Scer\UAS tumP417Q.Scer\UAS wing & cell, with Scer\GAL4Bx-MS1096 tumΔE1E.Scer\UAS tumP417Q.Scer\UAS wing & cell, with Scer\GAL4en-e16E tumΔE1E.Scer\UAS tumP417Q.Scer\UAS wing & trichome, with Scer\GAL469B tumΔE1E.Scer\UAS tumP417Q.Scer\UAS wing & trichome, with Scer\GAL4Bx-MS1096 tumΔE1E.Scer\UAS tumP417Q.Scer\UAS wing & trichome, with Scer\GAL4en-e16E tumΔE1E.Scer\UAS tumP417Q.Scer\UAS wing disc, with Scer\GAL4en-e16E tumΔE1E.Scer\UAS wing hair | posterior compartment, with Scer\GAL4en-e16E tumdsRNA.Scer\UAS.cSa wing vein, with Scer\GAL469B tumΔE1E.Scer\UAS tumP417Q.Scer\UAS wing vein, with Scer\GAL4Bx-MS1096 tumΔE1E.Scer\UAS tumP417Q.Scer\UAS tumScer\UAS.cSa wing vein, with Scer\GAL4C-765 tumScer\UAS.cSa wing vein, with Scer\GAL4en-e16E tumΔE1E.Scer\UAS tumP417Q.Scer\UAS tumScer\UAS.cSa
Classical Alleles ( 8 )
For All Classical Alleles Show
Allele of tum Class Mutagen Stocks Known lesion tumDH15 amorphic allele - genetic evidence, loss of function allele ethyl methanesulfonate 1 Yes tum347 1 Yes tumAR2 loss of function allele ethyl methanesulfonate 1 Yes tumEY01491b P-element activity 1 -- tum14 ethyl methanesulfonate 0 -- tum1 0 Yes tumdsRNA.H.Scer\UAS 0 -- tumunspecified 0 --
Alleles Carried on Transgenic Constructs ( 32 )
For All Alleles Carried on Transgenic Constructs Show
Allele of tum Class Mutagen Stocks Known lesion tumdsRNA.Scer\UAS.cBa in vitro construct - regulatory fusion in vitro construct - RNAi 1 Yes tumGD7990 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes tumHMS01417 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes tumJF01092 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes tumJF01093 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes tumJF01639 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes tumJF01678 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes tumKK109362 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes tumdsRNA.1110-1961 in vitro construct - RNAi 0 Yes tumdsRNA.cHa in vitro construct - RNAi 0 -- tumdsRNA.cRa in vitro construct - RNAi 0 Yes tumdsRNA.cSa in vitro construct - RNAi 0 Yes tumdsRNA.Scer\UAS.cSa in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes tumdsRNA.Scer\UAS in vitro construct - RNAi 0 Yes tumg in vitro construct - genomic fragment 0 Yes tumP417Q.Scer\UAS in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes tumR417L.Scer\UAS.T:Hsap\MYC,T:Avic\GFP-EYFP in vitro construct - site directed mutagenesis 0 Yes tumR417L in vitro construct - site directed mutagenesis 0 Yes tumScer\UAS.cSa in vitro construct - regulatory fusion 0 Yes tumScer\UAS.T:Avic\GFP-YFP.Venus in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes tumScer\UAS.T:Hsap\MYC,T:Avic\GFP-EYFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes tumScer\UAS.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes tumScer\UAS.Z.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes tumΔE1E.Scer\UAS in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes tumΔEIE.Scer\UAS.T:Hsap\MYC in vitro construct - site directed mutagenesis 0 Yes tumΔEIE.Scer\UAS in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes tumΔNLS.Scer\UAS 0 Yes tumΔPav.Scer\UAS in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes tumΔPbl.Scer\UAS in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes tumΔYRL.Scer\UAS.T:Hsap\MYC in vitro construct - site directed mutagenesis 0 Yes tumΔYRL.Scer\UAS in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes tumΔZ4 in vitro construct - deletion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)CX1 (Sotillos and Campuzano, 2000, Jones, 2005) Df(2R)Exel7128 (Ryder, 2004.4.26, Zavortink et al., 2005)
Not disrupted in	Df(2R)vg-B (Jones, 2005)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD7990} NA P{KK109362} NA P{TRiP.HMS01417} NA P{TRiP.JF01092} NA P{TRiP.JF01093} NA P{TRiP.JF01639} NA P{TRiP.JF01678} NA P{UAS-tum.MYC.Z} NA P{UAS-tum.MYC} NA P{UAS-tum.ΔEIE} NA P{UAS-tum.ΔNLS} NA P{UAS-tum.ΔPav} NA P{UAS-tum.ΔPbl} NA P{UAS-tum.ΔYRL} NA P{UAS-Venus-tum} NA characterization construct P{gTum} NA P{tum.R417} NA P{tum.ΔZ4} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}tumEY01491b NA
Gene Ontology: Function, Process & Cellular Component ( 20 unique terms )
Terms Based on Experimental Evidence ( 16 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
cell fate determination inferred from mutant phenotype (Jones et al., 2010) cell proliferation inferred from genetic interaction with Cdc42 (Sotillos and Campuzano, 2000) centrosome duplication inferred from mutant phenotype (Müller et al., 2010) cytokinesis inferred from mutant phenotype (Somma et al., 2002, Echard et al., 2004, Eggert et al., 2004, Goshima et al., 2007, Dean et al., 2005, Zavortink et al., 2005) dendrite morphogenesis inferred from mutant phenotype (Gao et al., 1999, Parrish et al., 2006) maintenance of protein location in cell inferred from mutant phenotype (Dean et al., 2005) mitotic spindle organization inferred from mutant phenotype (Goshima et al., 2007) negative regulation of Wnt receptor signaling pathway inferred from genetic interaction with Axn AND inferred from genetic interaction with nkd AND inferred from mutant phenotype (Jones, 2005) inferred from genetic interaction with nkd AND inferred from genetic interaction with wg (Jones et al., 2003) neurogenesis inferred from mutant phenotype (Neumüller et al., 2011) neuron development inferred from mutant phenotype (Parrish et al., 2006) regulation of Wnt receptor signaling pathway inferred from genetic interaction with arm (Jones et al., 2010) inferred from genetic interaction with pav (Jones et al., 2010) inferred from mutant phenotype (Jones et al., 2010) Rho protein signal transduction inferred from genetic interaction with aos (Sotillos and Campuzano, 2000) inferred from genetic interaction with Pak (Sotillos and Campuzano, 2000) inferred from genetic interaction with rho (Sotillos and Campuzano, 2000) inferred from genetic interaction with vn (Sotillos and Campuzano, 2000)
Cellular Component
CV term Evidence References
colocalizes_with cortical microtubule inferred from direct assay (Zavortink et al., 2005) colocalizes_with midbody inferred from direct assay (Zavortink et al., 2005) nucleus inferred from direct assay (Jones et al., 2010) colocalizes_with spindle inferred from direct assay (Zavortink et al., 2005)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
diacylglycerol binding inferred from sequence or structural similarity with InterPro:IPR002219 (FlyBase, 1992-) GTPase activator activity inferred from sequence or structural similarity with EMBL:AF079974 (FlyBase, 1992-) Rho GTPase activator activity non-traceable author statement (Sotillos, 1999.12.6) signal transducer activity inferred from sequence or structural similarity with EMBL:AF079974 (FlyBase, 1992-)
Biological Process ( 0 terms)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	gene   nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions tum allele Gene References RacGAP50CAR2 Axn (Jones, 2005) nkd (Jones, 2005) wg (Jones, 2005) RacGAP50CDH15 wg (Jones, 2005) RacGAP50CdsRNA.Scer\UAS.cSa pbl (Somers and Saint, 2003) RacGAP50CScer\UAS.cSa Rac1 (Sotillos and Campuzano, 2000) RacGAP50CΔE1E.Scer\UAS Cdc42 (Sotillos and Campuzano, 2000) Pak (Sotillos and Campuzano, 2000) phl (Sotillos and Campuzano, 2000) Rac1 (Sotillos and Campuzano, 2000) rho (Sotillos and Campuzano, 2000) unspecified aos (Sotillos and Campuzano, 2000) nkd (Jones et al., 2003) RacGAP84C (Sotillos and Campuzano, 2000) vn (Sotillos and Campuzano, 2000) wg (Jones et al., 2003)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 62292 DroID - A comprehensive database of gene and protein interactions. • FBgn0086356 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36538
Orthologs
Genome-wide drosophilid orthologs
Curated drosophilid orthologs	Dpse\GA12222
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL010342-PA Aedes aegypti (mosquito) AGAP008912-PA Anopheles gambiae (mosquito) XP_393627.2 Apis mellifera (honey bee) XP_001948256.1 Acyrthosiphon pisum (pea aphid) 125596 Branchiostoma floridae (lancelet) 126189 Branchiostoma floridae (lancelet) BGIBMGA014109-PA Bombyx mori (silk worm) ENSBTAP00000016988 Bos taurus (bovine) CN13182 Caenorhabditis brenneri (small nematode) ENSCAFP00000012284 Canis familiaris (dog) ENSCINP00000013386 Ciona intestinalis (sea squirt) CPIJ003168 Culex pipens (mosquito) ENSCPOP00000003956 Cavia porcellus (guinea pig) RP02890 Caenorhabditis remanei (nematode) 161236 Capitella spl (polychaete worm) 270847 Daphnia pulex (daphnia) ENSDARP00000020703 Danio rerio (zebra fish) ENSECAP00000009022 Equus caballus (horse) ENSGACP00000010880 Gymnopilus aculeatus (mushroom) ENSGALP00000010121 Gallus gallus (chicken) 177894 Hypsipyla robusta (cedar shoot borer) ENSP00000309871 Homo sapiens (human) ISCW008636-PA Ixodes scapularis (deer tick) 238160 Lottia gigantea (owl limpet) ENSMODP00000005345 Monodelphis domestica (gray opossum) ENSMMUP00000028558 Macaca mulatta (rhesus monkey) ENSMUSP00000023756 Mus musculus (house mouse) 238395 Nematostella vectensis (starlet anemone) XP_001605567.1 Nasonia vitripennis (wasp) ENSOANP00000018603 Ornithorynchus anatinus (platypus) ENSORLP00000019460 Oryzias latipes (japanese killifish) PHUM011569-PA Pediculus humanus (human body louse) PP20320 Pristionchus pacificus (nematode) ENSPPYP00000005141 Pongo pygmaeus (orangutan) ENSPTRP00000008406 Pan troglodytes (chimpanzee) ENSRNOP00000024796 Rattus norvegicus (norway rat) XP_001196518.1 Strongylocentrotus purpuratus (purple sea urchin) 55060 Trichoplax adhaerens (metazoa) XP_969805.1 Tribolium castaneum (red flour beetle) ENSTNIP00000015493 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000038479 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000029342 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV12258 LH17976 ISCW008636 FBgn0231016 FBgn0223337 FBgn0207830 FBgn0197018 FBgn0175133 JGI_V11_306528 JGI_V11_270847 JGI_V11_333128 FBgn0072268 FBgn0154597 FBgn0143706 FBgn0128326 FBgn0114635 FBgn0088199 CPIJ003168 BGIBMGA014109 ACYPI006700 ACYPI001735 ACYPI000850 GB18839 AGAP008912 AAEL010342 PB19673 PHUM613950 PHUM615410 GLEAN_10549
Stocks & Reagents
Stocks Listed in FlyBase ( 12 )
Bloomington	8686 cn1 tumAR2 bw1/CyO 28982 y1 v1; P{TRiP.JF01639}attP2 31173 y1 v1; P{TRiP.JF01678}attP2 31532 y1 v1; P{TRiP.JF01092}attP2 31533 y1 v1; P{TRiP.JF01093}attP2 8687 cn1 tumDH15 bw1/CyO 6439 y1 w*; P{UAS-RacGAP50C-dsRNA}1.2 35007 y1 sc* v1; P{TRiP.HMS01417}attP2 25711 y1 w*; P{neoFRT}42D tum347/CyO, y+ 15072 y1 w67c23; P{EPgy2}tumEY01491b P{EPgy2}shotEY01491a
VDRC	v106850 P{KK109362}VIE-260B v17145 w1118; P{GD7990}v17145
Genomic Clones ( 2 )
	BACR06M19 BACR16G12 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 124 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE37229
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT05249 AT08015 GM02876 GM22540 GM25733 LD01685 LD02333 LD02812 LD05426 LD06373 LD07777 LD08696 LD09729 LD10404 LD10433 LD10731 LD14186 LD14635 LD18181 LD18239 LD18342 LD18930 LD19435 LD20013 LD22090 LD23423 LD24090 LD25646 LD28642 LD29526 LD30535 LD30548 LD31248 LD37940 LD40287 LD41864 LD46180 RE02190 RE07418 RE07746 RE09330 RE16864 RE17695 RE24120 RE28608 RE41162 RE42329 RE44280 RE46672 RE50622 RE53746 RE62256 RE64204 RE67410 RE69287 RE69794 RE72849 RE73547 RE73811 RE74020 SD04150 SD04535 SD10166 SD10802 SD10831 SD13332 SD20653 SD20725 SD22222 SD22903 SD23661 SD23672 SD24308 SD24389 SD26804 SD27082 SD28054
Other clones	28453 29844 56883 61392 98300 173803 173976.1 174202.1 184487 214249.21 214473.18 282940 Dmel_dig_010755_0643_0739 EK136444 EK184857 EK220826 EK228705 EK242805 EK274517 EK278321 EK289423 EK308458 EP03833 EP05521 FGM323C07 LD25058 RP01129 RP01462 RP001001326 RP001007990 RP001008142 RP001010369 RP001010686 RP001011598 RP001014166 RP001016144 RP001018518 RP001022885 RP001024672 RP001028041 RP001038343 RP001045066 RP001049357 RP001052316 RP001053327 RP001053659
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0086356 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36538 247521
Antibody Information
	polyclonal (Zavortink et al., 2005)
Other Information
Discoverer
Etymology
	The mutation was named "enhancer of naked" after the mutant phenotype. (Jones et al., 2003)
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: acGAP CG13345 (FlyBase, 1996-) Source for identity of: RacGAP50C eon (Bejsovec, 2003.2.10)
Source for database merge of	Source for merge of: RacGAP50C tum (Goldstein, 2005)
Additional comments	Source for identity of acGAP CG13345 was sequence comparison (date:000721). (FlyBase, 1996-)
Other Comments
	RNAi screen using dsRNA made from templates generated with primers directed against this gene causes defects in spindle shape and cytokinesis failure when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA. (Goshima et al., 2007) dsRNA has been made from templates generated with primers directed against this gene. RNAi of tum results in supernumerary class I neurons and a range of arborization defects. (Parrish et al., 2006) RacGAP50C negatively regulates wg pathway activity during embryonic development. (Jones, 2005) dsRNA directed against this gene causes defects in cytokinesis when tested in an RNAi screen in S2 cells. (Echard et al., 2004) RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a binucleation phenotype when assayed in Kc167 cells. (Eggert et al., 2004) RacGAP50C alters the wg homozygous mutant cuticle pattern phenotype. The RacGAP50C mutation in a wild-type background reduces denticle diversity and produces a compression and lateral expansion of the denticle belts. In the first row of denticles in each belt, some or all of the denticles may be missing. RacGAP50C interacts genetically with the gene nkd, enhancing the nkd phenotype. (Jones et al., 2003) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation. (Rogers et al., 2003) Expression of RacGAP50C is repressed by Egfr/Ras signalling in the prospective wing veins and accumulates at the vein/intervein boundaries. (Sotillos and Campuzano, 2000) RacGAP50C acts as a negative regulator of Rho-family GTPases Rac1 and Cdc42. (Sotillos and Campuzano, 2000) Reduced RacGAP50C or increased Rac1 activity in the wing disc cause similar defects: widening of veins development of extra sensory organs, apoptosis and appearance of enlarged cells that differentiate multiple hairs with abnormal polarity. (Sotillos and Campuzano, 2000) tum has a function in dendritic development. (Gao et al., 1999) "49C--50D" was stated as revision. (Gao, 1999.11.4, Gao et al., 1999)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_137068 RefSeq (Proteins) • NP_610912 DDBJ / EMBL / GenBank • AAF58324 AAM50285 AC005977 AJ251502 AY119631 BI240389 BZ665555 CAB96203 Entrez Gene - A searchable database of RefSeq genes. • 36538 UniProtKB/TrEMBL • A1Z9I5 Q8MRH0 Q9N9Z9
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Rho GTPase activation protein (IPR008936) Protein kinase C-like, phorbol ester/diacylglycerol binding (IPR002219) Rho GTPase-activating protein domain (IPR000198)
Linkouts
	BioGRID - A database of protein and genetic interactions • 62292 DroID - A comprehensive database of gene and protein interactions. • FBgn0086356 DRSC - Results from RNAi screens. • FBgn0086356 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0086356 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG13345-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0086356 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36538 247521 InParanoid A subset of ortholog calls from InParanoid. • AAEL010342-PA Aedes aegypti (mosquito) AGAP008912-PA Anopheles gambiae (mosquito) XP_393627.2 Apis mellifera (honey bee) XP_001948256.1 Acyrthosiphon pisum (pea aphid) 125596 Branchiostoma floridae (lancelet) 126189 Branchiostoma floridae (lancelet) BGIBMGA014109-PA Bombyx mori (silk worm) ENSBTAP00000016988 Bos taurus (bovine) CN13182 Caenorhabditis brenneri (small nematode) ENSCAFP00000012284 Canis familiaris (dog) ENSCINP00000013386 Ciona intestinalis (sea squirt) CPIJ003168 Culex pipens (mosquito) ENSCPOP00000003956 Cavia porcellus (guinea pig) RP02890 Caenorhabditis remanei (nematode) 161236 Capitella spl (polychaete worm) 270847 Daphnia pulex (daphnia) ENSDARP00000020703 Danio rerio (zebra fish) ENSECAP00000009022 Equus caballus (horse) ENSGACP00000010880 Gymnopilus aculeatus (mushroom) ENSGALP00000010121 Gallus gallus (chicken) 177894 Hypsipyla robusta (cedar shoot borer) ENSP00000309871 Homo sapiens (human) ISCW008636-PA Ixodes scapularis (deer tick) 238160 Lottia gigantea (owl limpet) ENSMODP00000005345 Monodelphis domestica (gray opossum) ENSMMUP00000028558 Macaca mulatta (rhesus monkey) ENSMUSP00000023756 Mus musculus (house mouse) 238395 Nematostella vectensis (starlet anemone) XP_001605567.1 Nasonia vitripennis (wasp) ENSOANP00000018603 Ornithorynchus anatinus (platypus) ENSORLP00000019460 Oryzias latipes (japanese killifish) PHUM011569-PA Pediculus humanus (human body louse) PP20320 Pristionchus pacificus (nematode) ENSPPYP00000005141 Pongo pygmaeus (orangutan) ENSPTRP00000008406 Pan troglodytes (chimpanzee) ENSRNOP00000024796 Rattus norvegicus (norway rat) XP_001196518.1 Strongylocentrotus purpuratus (purple sea urchin) 55060 Trichoplax adhaerens (metazoa) XP_969805.1 Tribolium castaneum (red flour beetle) ENSTNIP00000015493 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000038479 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000029342 Xenopus tropicalis (frog) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /cytoskel/racgap50c-1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36538 modMine - Data generated by the modENCODE project. • FBgn0086356 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV12258 LH17976 ISCW008636 FBgn0231016 FBgn0223337 FBgn0207830 FBgn0197018 FBgn0175133 JGI_V11_306528 JGI_V11_270847 JGI_V11_333128 FBgn0072268 FBgn0154597 FBgn0143706 FBgn0128326 FBgn0114635 FBgn0088199 CPIJ003168 BGIBMGA014109 ACYPI006700 ACYPI001735 ACYPI000850 GB18839 AGAP008912 AAEL010342 PB19673 PHUM613950 PHUM615410 GLEAN_10549
Synonyms & Secondary IDs ( 21 )
Reported As
Symbol Synonym	13345 (Hu, 2005) AcGAP (Rogers et al., 2003, Rogers et al., 2003, Ramet et al., 2002) acGAP (Bernards, 2003, Somma et al., 2002, Somma et al., 2001, ) CG13345 (Rogers et al., 2003, Hu, 2005, Dimova et al., 2003, Battersby et al., 2003, Asha et al., 2003, Ramet et al., 2002, Billuart et al., 2001, Goshima et al., 2007, Keleman et al., 2009.8.5, Perkins et al., 2009, Ni et al., 2008, Montembault et al., 2010, Ni et al., 2011.7.19) DRacGAP (Billuart et al., 2001, Sotillos and Campuzano, 2000) eon (Jones et al., 2003) i249 (Battersby et al., 2003) RacGAP (Asha et al., 2003, ) RacGap (Ni et al., 2009) racGAP50 (Somma et al., 2008) racGAP50C (Hickson et al., 2006, Echard et al., 2004, Jones and Saint, 2005) RacGap50C (Jones and Bejsovec, 2007, Jones, 2005, Bejsovec, 2005.1, Jones et al., 2004, Jones and Bejsovec, 2006, Jang et al., 2005) RacGAP50C (Bakal et al., 2007, Guerin et al., 2008, Gregory et al., 2008, Bakal et al., 2007, D'Avino et al., 2005, Georlette et al., 2007, Ng, 2008, Gregory et al., 2008, Zavortink et al., 2005, D'Avino et al., 2008, D'Avino et al., 2006, Nir et al., 2010, Serio et al., 2010, Jones et al., 2010, Montembault et al., 2010, Lee and Thomas, 2011, Ebrahimi et al., 2010) RacGAP50c (Goshima et al., 2007) racGAP50c (Dean et al., 2005) RhoGAP-50C14 (Billuart et al., 2001) tum (Guerin et al., 2008, Ng, 2008, Zavortink et al., 2005, Jones et al., 2010, Neumüller et al., 2011, Müller et al., 2010) TUM (Moshkin et al., 2009)
Name Synonym	enhancer of naked (Jones et al., 2003) RacGAP50C   RacGap50C (Ni et al., 2009) tumbleweed (Parrish et al., 2006, Gao et al., 1999, Guerin et al., 2008, Jones et al., 2010) Tumbleweed (Prokopenko and Chia, 2007, Prokopenko and Chia, 2008, Zavortink et al., 2005)
Secondary FlyBase IDs
	FBgn0028559 FBgn0033881
References ( 83 )
Generate a list of	All references relating to this gene ( 83 )
List References by type	Research paper  ( 39 ) Supplementary material  ( 9 ) Review  ( 6 ) Erratum  ( 1 ) Personal communication to FlyBase  ( 8 ) Abstract  ( 12 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 1 ) Computer file  ( 2 ) Conference report  ( 1 ) Automatic genome annotation  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 9 )
	Lee and Thomas, 2011, Mech. Dev. 128(1-2): 116--128 Rac1 modulation of the apical domain is negatively regulated by β(Heavy)-spectrin. [FBrf0213021] Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593 Genome-Wide Analysis of Self-Renewal in Drosophila Neural Stem Cells by Transgenic RNAi. [FBrf0213621] Blanco et al., 2010, BMC Dev. Biol. 10: 94 Gene expression following induction of regeneration in Drosophila wing imaginal discs. Expression profile of regenerating wing discs. [FBrf0211812] Ebrahimi et al., 2010, J. Biol. Chem. 285(37): 28667--28673 Polo kinase interacts with RacGAP50C and is required to localize the cytokinesis initiation complex. [FBrf0214097] Jones et al., 2010, J. Cell Sci. 123(13): 2179--2189 Cytokinesis proteins Tum and Pav have a nuclear role in Wnt regulation. [FBrf0211056] Montembault et al., 2010, J. Cell Biol. 191(7): 1351--1365 Nessun Dorma, a novel centralspindlin partner, is required for cytokinesis in Drosophila spermatocytes. [FBrf0212581] Müller et al., 2010, EMBO J. 29(19): 3344--3357 Proteomic and functional analysis of the mitotic Drosophila centrosome. [FBrf0212030] Nir et al., 2010, Genome Res. 20(3): 372--380 Inference of RhoGAP/GTPase regulation using single-cell morphological data from a combinatorial RNAi screen. [FBrf0210162] Serio et al., 2010, Cell Host Microbe 7(5): 388--398 Defining a Core Set of Actin Cytoskeletal Proteins Critical for Actin-Based Motility of Rickettsia. [FBrf0210774] Show all research papers ...
Recent reviews ( 1 )
	Gregory et al., 2010, IUBMB Life 62(4): 290--295 Signalling through the RhoGEF Pebble in Drosophila. [FBrf0210431] Show all reviews ...