FlyBase Gene Report: Dmel\alph

General Information

Symbol

Dmel\alph

Species

D. melanogaster

Name

alphabet

Annotation Symbol

CG1906

Feature Type

protein_coding_gene

FlyBase ID

FBgn0086361

Gene Model Status

Current

Stock Availability

12 publicly available

Enzyme Name (EC)

Protein-serine/threonine phosphatase (3.1.3.16)

Gene Summary

alphabet (alph) encodes a Ser/Thr phosphatase that regulates RAS/MAPK signaling. [Date last reviewed: 2019-08-01] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Pathway
Gene Group
Interactive Fly

Also Known As

SK3-1, pp2c99B

Key Links

Genomic Location

Cytogenetic map

99B4-99B5

Sequence location

3R:29,591,831..29,617,157 [+]

Recombination map

3-99

RefSeq locus

NT_033777 REGION:29591831..29617157

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (22 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables protein serine/threonine phosphatase activity

inferred from direct assay

(Baril and Therrien, 2006)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables magnesium ion binding

inferred from electronic annotation with InterPro:IPR012911

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables manganese ion binding

inferred from electronic annotation with InterPro:IPR012911

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Biological Process (17 terms)

Terms Based on Experimental Evidence (17 terms)

CV Term

Evidence

References

involved_in dephosphorylation

inferred from direct assay

(Baril and Therrien, 2006)

involved_in determination of adult lifespan

inferred from mutant phenotype

(Baril et al., 2009)

involved_in dorsal closure

inferred from genetic interaction with FLYBASE:slpr; FB:FBgn0030018

(Baril et al., 2009)

involved_in imaginal disc fusion, thorax closure

inferred from genetic interaction with FLYBASE:slpr; FB:FBgn0030018

(Baril et al., 2009)

involved_in mitotic cell cycle

inferred from mutant phenotype

(Chen et al., 2007)

involved_in negative regulation of ERK1 and ERK2 cascade

inferred from mutant phenotype

(Baril and Therrien, 2006)

inferred from high throughput genetic interaction with FLYBASE:Ras85D; FB:FBgn0003205

(Ashton-Beaucage et al., 2014)

involved_in negative regulation of JNK cascade

inferred from mutant phenotype

(Baril et al., 2009)

inferred from genetic interaction with FLYBASE:hep; FB:FBgn0010303

(Baril et al., 2009)

involved_in negative regulation of photoreceptor cell differentiation

inferred from genetic interaction with FLYBASE:aop; FB:FBgn0000097

(Baril and Therrien, 2006)

involved_in negative regulation of R7 cell differentiation

inferred from genetic interaction with FLYBASE:aop; FB:FBgn0000097

(Baril and Therrien, 2006)

involved_in negative regulation of Ras protein signal transduction

inferred from high throughput genetic interaction with FLYBASE:Ras85D; FB:FBgn0003205

(Ashton-Beaucage et al., 2014)

involved_in negative regulation of sevenless signaling pathway

inferred from genetic interaction with FLYBASE:aop; FB:FBgn0000097

(Baril and Therrien, 2006)

involved_in negative regulation of stress-activated MAPK cascade

inferred from genetic interaction with FLYBASE:hep; FB:FBgn0010303

(Baril et al., 2009)

inferred from genetic interaction with FLYBASE:slpr; FB:FBgn0030018

(Baril et al., 2009)

inferred from genetic interaction with FLYBASE:lic; FB:FBgn0261524

(Baril et al., 2009)

involved_in negative regulation of stress-activated protein kinase signaling cascade

inferred from mutant phenotype

(Baril et al., 2009)

inferred from genetic interaction with FLYBASE:Rac1; FB:FBgn0010333

(Baril et al., 2009)

involved_in regulation of p38MAPK cascade

inferred from genetic interaction with FLYBASE:lic; FB:FBgn0261524

(Baril et al., 2009)

involved_in response to oxidative stress

inferred from mutant phenotype

(Baril et al., 2009)

involved_in response to paraquat

inferred from mutant phenotype

(Baril et al., 2009)

involved_in response to UV-C

inferred from mutant phenotype

(Baril et al., 2009)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in negative regulation of stress-activated MAPK cascade

inferred from biological aspect of ancestor with PANTHER:PTN002675704

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in cytosol

inferred from biological aspect of ancestor with PANTHER:PTN002302296

(assigned by GO_Central )

(Gaudet et al., 2011)

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN002302296

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

PPM FAMILY PROTEIN SERINE/THREONINE PHOSPHATASES

Pathway (FlyBase)

Negative Regulators of Sevenless Signaling Pathway

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC)

Experimental Evidence

[a protein]-serine/threonine phosphate + H(2)O = [a protein]- serine/threonine + phosphate (3.1.3.16)

Predictions / Assertions

Summaries

Gene Snapshot

alphabet (alph) encodes a Ser/Thr phosphatase that regulates RAS/MAPK signaling. [Date last reviewed: 2019-08-01]

Gene Group (FlyBase)

PPM FAMILY PROTEIN SERINE/THREONINE PHOSPHATASES -: Metal-dependent protein phosphatase (PPM) are Mg²⁺- or Mn²⁺-dependent serine/threonine phosphatases. The phosphatase does not require regulatory subunits, but has additional domains that may determine substrate specificity. (Adapted from PMID:11230548 and PMID:19879837).

Pathway (FlyBase)

Negative Regulators of Sevenless Signaling Pathway -: Negative regulators of Sevenless signaling down-regulate the pathway, directing the presumptive R7 cell fate to that of a cone cell. (Adapted from FBrf0127283 and FBrf0221727).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\alph for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.40

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.50

EST data suggest additional 3' exon(s)

Sequence Ontology: Class of Gene

gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

alph-RA

FBtr0085442

NM_170416

2576

368

alph-RB

FBtr0085443

NM_170419

2712

371

alph-RC

FBtr0085446

NM_170417

2341

368

alph-RD

FBtr0085445

NM_170418

2417

368

alph-RE

FBtr0085444

NM_143444

1683

374

alph-RF

FBtr0309241

NM_001260427

2504

332

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

alph-PA

FBpp0084808

41.0

368

4.95

NP_733295

AAF56905

alph-PB

FBpp0084809

41.4

371

4.77

NP_733298

AAN14179

alph-PC

FBpp0084812

41.0

368

4.95

NP_733296

AAN14177

alph-PD

FBpp0084811

41.0

368

4.95

NP_733297

AAN14178

alph-PE

FBpp0084810

41.6

374

4.88

NP_651701

AAN14176

alph-PF

FBpp0301180

36.9

332

5.47

NP_001247356

AFH06673

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

368 aa isoforms: alph-PA, alph-PC, alph-PD

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\alph using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

alph_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\alph in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\alph

View exonic expression by tissue for Dmel\alph

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

43481/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0086361

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0086361

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0086361

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1906

Flygut - An atlas of the Drosophila adult midgut

FBgn0086361

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 9 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 10 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of alph

Transgenic constructs containing regulatory region of alph

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(3R)Dr-rv1

(Therrien et al., 2000)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | recessive

partially lethal - majority live (with alph^S-331)

alph^S-355

partially lethal - majority live (with alph^S-355)

partially lethal - majority live (with alph^XS-88)

alph^S-355

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

alph^GD8826

viable, with Scer\GAL4^Mef2.PR

alph^GD8826

viable, with Scer\GAL4^pnr-MD237

alph^GD8826

viable, with Scer\GAL4^tin.CΔ4

alph^GD8826

Sterility

Allele

female semi-sterile, with Scer\GAL4^{VP16.mat.αTub67C}

alph^HMS02040

fertile

alph^c04528

Other Phenotypes

Allele

abnormal oxidative stress response

long lived

radiation sensitive

radiation sensitive, with Scer\GAL4^Tub.PU

alph^dsRNA.UAS

visible (with alph^S-331)

alph^S-355

visible (with alph^S-355)

visible (with alph^XS-88)

alph^S-355

wild-type

Phenotype manifest in

Allele

cone cell

alph^S-331

embryonic/larval fat body | third instar larval stage, with Scer\GAL4^{SPARC-MI00329-GAL4}

alph^HMS02040

extracellular matrix | third instar larval stage, with Scer\GAL4^{SPARC-MI00329-GAL4}

alph^HMS02040

eye (with alph^S-331)

alph^S-355

eye (with alph^S-355)

eye (with alph^XS-88)

alph^S-355

photoreceptor cell R7 | increased number

primary pigment cell

alph^S-331

secondary pigment cell | increased number

alph^S-331

tertiary pigment cell | increased number

alph^S-331

wing (with alph^S-331)

alph^S-355

wing (with alph^S-355)

wing (with alph^XS-88)

alph^S-355

wing vein (with alph^S-331)

alph^S-355

wing vein (with alph^S-355)

wing vein (with alph^XS-88)

alph^S-355

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (18)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\PPM1A

13 of 15

Yes

Hsap\PPM1B

12 of 15

Yes

Hsap\PPM1N

7 of 15

Yes

3 of 15

3 of 15

2 of 15

2 of 15

Yes

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (18)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Ppm1a

13 of 15

Yes

Mmus\Ppm1b

13 of 15

Yes

Mmus\Ppm1n

7 of 15

Yes

Mmus\Ppm1f

3 of 15

Mmus\Pdp2

2 of 15

Mmus\Pp2d1

2 of 15

Yes

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Rattus norvegicus (Norway rat) (17)

Rnor\Ppm1a

11 of 13

Yes

Rnor\Ppm1b

10 of 13

Yes

Rnor\Ppm1n

5 of 13

Yes

Rnor\Ppm1g

3 of 13

Rnor\Pdp2

2 of 13

Rnor\Pp2d1

2 of 13

Yes

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Xenopus tropicalis (Western clawed frog) (15)

Xtro\ppm1b

12 of 12

Yes

Xtro\ppm1a

11 of 12

Yes

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Danio rerio (Zebrafish) (23)

Drer\ppm1ab

12 of 15

Yes

Drer\ppm1ba

12 of 15

Yes

Drer\ppm1bb

12 of 15

Yes

Drer\ppm1aa

10 of 15

Yes

Drer\ppm1na

7 of 15

Yes

Drer\ppm1nb

7 of 15

Yes

3 of 15

3 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Drer\si:ch211-149b19.3

1 of 15

Drer\tab1

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (9)

Cele\ppm-1.A

15 of 15

Yes

4 of 15

3 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Arabidopsis thaliana (thale-cress) (15)

Atha\AT2G25070

3 of 9

Yes

Atha\AT4G31860

3 of 9

Yes

Atha\ABI1

1 of 9

Yes

Atha\ABI2

1 of 9

Yes

Atha\AHG1

1 of 9

Yes

Atha\AT4G11040

1 of 9

Yes

Atha\DBP1

1 of 9

Yes

Atha\HAB1

1 of 9

Yes

Atha\HAB2

1 of 9

Yes

Atha\HAI1

1 of 9

Yes

Atha\HAI2

1 of 9

Yes

Atha\HAI3

1 of 9

Yes

Atha\PP2CA

1 of 9

Yes

Atha\PP2CG1

1 of 9

Yes

Atha\TAP38

1 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (7)

Scer\PTC2

5 of 15

Yes

Scer\PTC1

3 of 15

Yes

3 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Schizosaccharomyces pombe (Fission yeast) (3)

Spom\ptc2

3 of 12

Yes

Spom\ptc3

3 of 12

Yes

Spom\ptc1

1 of 12

Yes

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190AT3 )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Dsuz\DS10_00012969

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA15122

Drosophila persimilis

Dper\GL13894

Drosophila willistoni

Dwil\GK11903

Drosophila virilis

Dvir\GJ10608

Drosophila mojavensis

Dmoj\GI23119

Drosophila grimshawi

Dgri\GH18902

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091507O1 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17012971

Glossina morsitans

Tsetse fly

Gmor\GMOY007008

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_11666

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL003326

Anopheles darlingi

American malaria mosquito

Adar\ADAC007994

Anopheles gambiae

Malaria mosquito

Agam\AGAP008149

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ004872

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W082D )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA004743

Danaus plexippus

Monarch butterfly

Dple\DPOGS214749-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL006010

Apis florea

Little honeybee

Aflo\3170936452

Apis mellifera

Western honey bee

Amel\GB52229

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP11405

Bombus terrestris

Buff-tailed bumblebee

Bter\15216253

Linepithema humile

Argentine ant

Lhum\LH14091

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG003524

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_05285

Tribolium castaneum

Red flour beetle

Tcas\TC015925

Pediculus humanus

Human body louse

Phum\PHUM335180

Rhodnius prolixus

Kissing bug

Rpro\RPRC009605

Cimex lectularius

Bed bug

Clec\CLEC003309

Cimex lectularius

Bed bug

Clec\CLEC005985

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_07159

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X07Y6 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR003078

Ixodes scapularis

Black-legged tick

Isca\ISCW008982

Stegodyphus mimosarum

African social velvet spider

Smim\KFM77742.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur24g01750

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_307949

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0AJQ )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Ppm1a/b

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Ppm1g

Ciona intestinalis

Vase tunicate

Cint\LOC100178049

Ciona intestinalis

Vase tunicate

Cint\LOC100183363

Gallus gallus

Domestic chicken

Ggal\PPM1A

Gallus gallus

Domestic chicken

Ggal\PPM1B

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (12)

Gene Symbol

Score

Source

Alignment

7 of 10

7 of 10

7 of 10

5 of 10

4 of 10

3 of 10

Pdp

3 of 10

3 of 10

2 of 10

1 of 10

1 of 10

fig

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PPM1A; protein phosphatase, Mg2+/Mn2+ dependent 1A

13 of 15

606108

PPM1B; protein phosphatase, Mg2+/Mn2+ dependent 1B

12 of 15

603770

PPM1N; protein phosphatase, Mg2+/Mn2+ dependent 1N (putative)

7 of 15

PPM1F; protein phosphatase, Mg2+/Mn2+ dependent 1F

3 of 15

619309

PPM1G; protein phosphatase, Mg2+/Mn2+ dependent 1G

3 of 15

605119

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

alph - Tak1

anti tag coimmunoprecipitation, western blot

(Baril et al., 2009)

alph - slpr

anti tag coimmunoprecipitation, western blot

(Baril et al., 2009)

alph - wnd

anti tag coimmunoprecipitation, western blot

(Baril et al., 2009)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

alph

suppressible

hep

(Baril et al., 2009)

alph

enhanceable

PTP-ER

(Baril and Therrien, 2006)

alph

enhanceable

RasGAP1

(Baril and Therrien, 2006)

alph

enhanceable

(Baril and Therrien, 2006)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

aop

enhanceable

alph

(Baril and Therrien, 2006)

hep

suppressible

alph

(Baril et al., 2009)

ksr::tor

suppressible

alph

(Baril and Therrien, 2006, Therrien et al., 2000)

lic

suppressible

alph

(Baril et al., 2009)

PTP-ER

enhanceable

alph

(Baril and Therrien, 2006)

Rac1

suppressible

alph

(Baril et al., 2009)

Rac1

enhanceable

alph

(Baril et al., 2009)

Raf

suppressible

alph

(Therrien et al., 2000)

Ras85D

enhanceable

alph

(Baril and Therrien, 2006, Therrien et al., 2000)

enhanceable

alph

(Baril and Therrien, 2006)

slpr

suppressible

alph

(Baril et al., 2009)

slpr

enhanceable

alph

(Baril et al., 2009)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

68345

DroID - A comprehensive database of gene and protein interactions.

FBgn0086361

MIST (genetic) - An integrated Molecular Interaction Database

alph

MIST (protein-protein) - An integrated Molecular Interaction Database

alph

Pathways

Signaling Pathways (FlyBase)

Negative Regulators of Sevenless Signaling Pathway -: Negative regulators of Sevenless signaling down-regulate the pathway, directing the presumptive R7 cell fate to that of a cone cell. (Adapted from FBrf0127283 and FBrf0221727).

Metabolic Pathways

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0086361

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

MAPK signaling pathway - fly

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-99

Cytogenetic map

99B4-99B5

Sequence location

3R:29,591,831..29,617,157 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

99B4-99B5

Limits computationally determined from genome sequence between P{PZ}l(3)06743⁰⁶⁷⁴³ and P{PZ}ncd⁰⁵⁸⁸⁴

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

99A-99B

(Therrien et al., 2000)

Experimentally Determined Recombination Data

Location

3-99

(FlyBase, 2016)

3-96.6

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (12)

Bloomington

59007

alph^XS-88

59009

alph^S-331

66166

w^*; P{UASp-GFP.alph}attP2

11492

w¹¹¹⁸; PBac{PB}alph^c04528

31398

y¹ v¹; P{TRiP.JF01192}attP2

59008

alph^S-355/TM3, P{sE-KDN.T}3, Sb¹

51234

y¹ w^*; Mi{MIC}alph^MI08597

23341

y¹ w^67c23; Mi{ET1}alph^MB01683

40873

y¹ sc^* v¹ sev²¹; P{TRiP.HMS02040}attP2

FlyORF

F003144

M{UAS-alph.ORF.3xHA.GW}ZH-86Fb

VDRC

v105483

P{KK107730}VIE-260B

v32476

w¹¹¹⁸; P{GD8826}v32476

Genomic Clones (35)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (169)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD23542

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0086361

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0086361

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

43481

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: SK3-1 pp2c99B

(Baril and Therrien, 2004)

Source for database merge of

Source for merge of: alph pp2c99B

(Baril and Therrien, 2006)

Source for merge of: alph CG1906

(Baril and Therrien, 2006)

Additional comments

Other Comments

Encodes a Ser/Thr phosphatase that is highly homologous to mammalian PP2C.

(Baril and Therrien, 2006)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 61 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

43481

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0086361

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

43481/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0086361

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

alph_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0086361

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0086361

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1906

Flygut - An atlas of the Drosophila adult midgut

FBgn0086361

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0086361

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

43481

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC015925

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG1906

MARRVEL_MODEL - MARRVEL (model organism gene)

43481

modMine - A data warehouse for the modENCODE project

FBgn0086361

Linkouts

BioGRID - A database of protein and genetic interactions.

68345

DroID - A comprehensive database of gene and protein interactions.

FBgn0086361

DRSC - Results frm RNAi screens

FBgn0086361

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0086361

FlyMine - An integrated database for Drosophila genomics

FBgn0086361

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

MAPK signaling pathway - fly

MIST (genetic) - An integrated Molecular Interaction Database

alph

MIST (protein-protein) - An integrated Molecular Interaction Database

alph

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

Name Synonyms

CG1906

alphabet

(Ashton-Beaucage and Therrien, 2017, Baril and Therrien, 2006, Baril and Therrien, 2006)

pp2c99B

Secondary FlyBase IDs

FBgn0084988
FBgn0043570
FBgn0039672

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (68)

Report Sections

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