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Gene Dmel\spn-F

General Information
Symbol	Dmel\spn-F	Species	D. melanogaster
Name	spn-F	Annotation symbol	CG12114
Feature type	protein_coding_gene	FlyBase ID	FBgn0086362
Gene Model Status	Current	Stock availability	3 publicly available
Genomic Location
Chromosome (arm)	3R	Recombination map	3-100
Cytogenetic map	100B8-100B9	Sequence location	3R:27,080,864..27,082,388 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene spn-F is referred to in FlyBase by the symbol Dmel\spn-F (CG12114, FBgn0086362). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: minus-end-directed microtubule motor activity. There is experimental evidence that it is involved in the biological process: oocyte axis specification; oocyte microtubule cytoskeleton organization; chaeta morphogenesis; regulation of cytoskeleton organization. 11 alleles are reported. The phenotypes of these alleles are annotated with: embryonic head; egg; macrochaeta; microtubule cytoskeleton; scutellar bristle; actin tubule; oocyte nucleus; dorsal appendage; embryonic abdominal segment. It has one annotated transcript and one annotated polypeptide. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, adult thoracico-abdominal ganglion, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval carcass. Comments on Affy2 ProbeSet: ProbeSet 1629151_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of spn-F. Gene sequence location is 3R:27080864..27082388.
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	spn-F: spindle F Maternal-effect lethal. Similar to spn-A.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2012_01	References Dubin-Bar et al., 2011, Development 138(21): 4661--4671 Sequence features HFA14475 BKN23984
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 100B8-100B9   Limits computationally determined from genome sequence between P{PZ}Aph-407028&P{PZ}dcorK215 and P{PZ}l(3)rM731rM731
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location	3-100 (Tearle and Nusslein-Volhard, 1987)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\spn-F for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) spn-F-RA FBtr0085760  NM_143601   1457   364
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein spn-F-PA   FBpp0085122   40.9   364   4.96     NP_651858   AAF57139
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA390937   AA538608   AA816416   AA941000   AA951502   AE014297 AAF57139   AW941475   AY061046 AAL28594   BF494722   BI563710   BI566689   BI566748   BI570444   BI575062   BI583365   BI587859   BI589277   BI589656   BI605788   BI610960   BI621651   BI623395   BI630890   BI632748   BI634021   CO182663   CO185194   CO283783   CO315772   CO332615   CO337625   EC077857   EC216637   EC264313   EL882870   GH941503   GH954174   GH955476   GH956075   GH958344   GH958465   GH962134   GH962775   GH963363   GH963412   GH964043   GH964858   GH964989   GH965128   GH969606   GH971762   GH973483   GH977945   GH978608   GH981601   GH983169
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9V9Y9
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0086362    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0086362 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0086362 expression level Developmental Stage   Expression Level embryo 00-02hr    5276 embryo 02-04hr    3092 embryo 04-06hr    2241 embryo 06-08hr    2076 embryo 08-10hr    2508 embryo 10-12hr    2025 embryo 12-14hr    1818 embryo 14-16hr    1721 embryo 16-18hr    1510 embryo 18-20hr    1026 embryo 20-22hr    1557 embryo 22-24hr    1648 larva L1    626 larva L2    675 larva L3 12hr old    495 larva L3 puffstage 1-2    1116 larva L3 puffstage 3-6    1545 larva L3 puffstage 7-9    2309 white prepupae new    2482 white prepupae 12hr    1589 white prepupae 24hr    1866 pupae 2d postWPP    1675 pupae 3d postWPP    1393 pupae 4d postWPP    1300 adult male 01day    1719 adult male 05day    2176 adult male 30day    1962 adult female 01day    1515 adult female 05day    2669 adult female 30day    2715 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (5276) embryo 02-04hr  (3092) embryo 04-06hr  (2241) embryo 06-08hr  (2076) embryo 08-10hr  (2508) embryo 10-12hr  (2025) embryo 12-14hr  (1818) embryo 14-16hr  (1721) embryo 16-18hr  (1510) embryo 18-20hr  (1026) embryo 20-22hr  (1557) embryo 22-24hr  (1648) larva L1  (626) larva L2  (675) larva L3 12hr old  (495) larva L3 puffstage 1-2  (1116) larva L3 puffstage 3-6  (1545) larva L3 puffstage 7-9  (2309) white prepupae new  (2482) white prepupae 12hr  (1589) white prepupae 24hr  (1866) pupae 2d postWPP  (1675) pupae 3d postWPP  (1393) pupae 4d postWPP  (1300) adult male 01day  (1719) adult male 05day  (2176) adult male 30day  (1962) adult female 01day  (1515) adult female 05day  (2669) adult female 30day  (2715) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (5276) embryo 02-04hr  (3092) embryo 04-06hr  (2241) embryo 06-08hr  (2076) embryo 08-10hr  (2508) embryo 10-12hr  (2025) embryo 12-14hr  (1818) embryo 14-16hr  (1721) embryo 16-18hr  (1510) embryo 18-20hr    1026 embryo 20-22hr  (1557) embryo 22-24hr  (1648) larva L1    626 larva L2    675 larva L3 12hr old    495 larva L3 puffstage 1-2    1116 larva L3 puffstage 3-6  (1545) larva L3 puffstage 7-9  (2309) white prepupae new  (2482) white prepupae 12hr  (1589) white prepupae 24hr  (1866) pupae 2d postWPP  (1675) pupae 3d postWPP    1393 pupae 4d postWPP    1300 adult male 01day  (1719) adult male 05day  (2176) adult male 30day  (1962) adult female 01day  (1515) adult female 05day  (2669) adult female 30day  (2715) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    5276 embryo 02-04hr    3092 embryo 04-06hr    2241 embryo 06-08hr    2076 embryo 08-10hr    2508 embryo 10-12hr    2025 embryo 12-14hr    1818 embryo 14-16hr    1721 embryo 16-18hr    1510 embryo 18-20hr    1026 embryo 20-22hr    1557 embryo 22-24hr    1648 larva L1    626 larva L2    675 larva L3 12hr old    495 larva L3 puffstage 1-2    1116 larva L3 puffstage 3-6    1545 larva L3 puffstage 7-9    2309 white prepupae new    2482 white prepupae 12hr    1589 white prepupae 24hr    1866 pupae 2d postWPP    1675 pupae 3d postWPP    1393 pupae 4d postWPP    1300 adult male 01day    1719 adult male 05day    2176 adult male 30day    1962 adult female 01day    1515 adult female 05day    2669 adult female 30day    2715 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    5276 embryo 02-04hr    3092 embryo 04-06hr    2241 embryo 06-08hr    2076 embryo 08-10hr    2508 embryo 10-12hr    2025 embryo 12-14hr    1818 embryo 14-16hr    1721 embryo 16-18hr    1510 embryo 18-20hr    1026 embryo 20-22hr    1557 embryo 22-24hr    1648 larva L1    626 larva L2    675 larva L3 12hr old    495 larva L3 puffstage 1-2    1116 larva L3 puffstage 3-6    1545 larva L3 puffstage 7-9    2309 white prepupae new    2482 white prepupae 12hr    1589 white prepupae 24hr    1866 pupae 2d postWPP    1675 pupae 3d postWPP    1393 pupae 4d postWPP    1300 adult male 01day    1719 adult male 05day    2176 adult male 30day    1962 adult female 01day    1515 adult female 05day    2669 adult female 30day    2715 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0086362 expression level Developmental Stage   Expression Level embryo 00-02hr    5276 embryo 02-04hr    3092 embryo 04-06hr    2241 embryo 06-08hr    2076 embryo 08-10hr    2508 embryo 10-12hr    2025 embryo 12-14hr    1818 embryo 14-16hr    1721 embryo 16-18hr    1510 embryo 18-20hr    1026 embryo 20-22hr    1557 embryo 22-24hr    1648 larva L1    626 larva L2    675 larva L3 12hr old    495 larva L3 puffstage 1-2    1116 larva L3 puffstage 3-6    1545 larva L3 puffstage 7-9    2309 white prepupae new    2482 white prepupae 12hr    1589 white prepupae 24hr    1866 pupae 2d postWPP    1675 pupae 3d postWPP    1393 pupae 4d postWPP    1300 adult male 01day    1719 adult male 05day    2176 adult male 30day    1962 adult female 01day    1515 adult female 05day    2669 adult female 30day    2715 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (5276) embryo 02-04hr  (3092) embryo 04-06hr  (2241) embryo 06-08hr  (2076) embryo 08-10hr  (2508) embryo 10-12hr  (2025) embryo 12-14hr  (1818) embryo 14-16hr  (1721) embryo 16-18hr  (1510) embryo 18-20hr  (1026) embryo 20-22hr  (1557) embryo 22-24hr  (1648) larva L1  (626) larva L2  (675) larva L3 12hr old  (495) larva L3 puffstage 1-2  (1116) larva L3 puffstage 3-6  (1545) larva L3 puffstage 7-9  (2309) white prepupae new  (2482) white prepupae 12hr  (1589) white prepupae 24hr  (1866) pupae 2d postWPP  (1675) pupae 3d postWPP  (1393) pupae 4d postWPP  (1300) adult male 01day  (1719) adult male 05day  (2176) adult male 30day  (1962) adult female 01day  (1515) adult female 05day  (2669) adult female 30day  (2715) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (5276) embryo 02-04hr  (3092) embryo 04-06hr  2241 embryo 06-08hr  2076 embryo 08-10hr  (2508) embryo 10-12hr  2025 embryo 12-14hr  1818 embryo 14-16hr  1721 embryo 16-18hr  1510 embryo 18-20hr    1026 embryo 20-22hr  1557 embryo 22-24hr  1648 larva L1    626 larva L2    675 larva L3 12hr old    495 larva L3 puffstage 1-2    1116 larva L3 puffstage 3-6  1545 larva L3 puffstage 7-9  2309 white prepupae new  (2482) white prepupae 12hr  1589 white prepupae 24hr  1866 pupae 2d postWPP  1675 pupae 3d postWPP    1393 pupae 4d postWPP    1300 adult male 01day  1719 adult male 05day  2176 adult male 30day  1962 adult female 01day  1515 adult female 05day  (2669) adult female 30day  (2715) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    5276 embryo 02-04hr    3092 embryo 04-06hr    2241 embryo 06-08hr    2076 embryo 08-10hr    2508 embryo 10-12hr    2025 embryo 12-14hr    1818 embryo 14-16hr    1721 embryo 16-18hr    1510 embryo 18-20hr    1026 embryo 20-22hr    1557 embryo 22-24hr    1648 larva L1    626 larva L2    675 larva L3 12hr old    495 larva L3 puffstage 1-2    1116 larva L3 puffstage 3-6    1545 larva L3 puffstage 7-9    2309 white prepupae new    2482 white prepupae 12hr    1589 white prepupae 24hr    1866 pupae 2d postWPP    1675 pupae 3d postWPP    1393 pupae 4d postWPP    1300 adult male 01day    1719 adult male 05day    2176 adult male 30day    1962 adult female 01day    1515 adult female 05day    2669 adult female 30day    2715 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    5276 embryo 02-04hr    3092 embryo 04-06hr    2241 embryo 06-08hr    2076 embryo 08-10hr    2508 embryo 10-12hr    2025 embryo 12-14hr    1818 embryo 14-16hr    1721 embryo 16-18hr    1510 embryo 18-20hr    1026 embryo 20-22hr    1557 embryo 22-24hr    1648 larva L1    626 larva L2    675 larva L3 12hr old    495 larva L3 puffstage 1-2    1116 larva L3 puffstage 3-6    1545 larva L3 puffstage 7-9    2309 white prepupae new    2482 white prepupae 12hr    1589 white prepupae 24hr    1866 pupae 2d postWPP    1675 pupae 3d postWPP    1393 pupae 4d postWPP    1300 adult male 01day    1719 adult male 05day    2176 adult male 30day    1962 adult female 01day    1515 adult female 05day    2669 adult female 30day    2715 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0086362    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0086362 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, adult thoracico-abdominal ganglion, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0086362 expression level Tissue   Expression Level Larval Central Nervous System    76.225 Larval Midgut    103.4 Larval Hindgut    100.4 Larval Malpighian Tubules    155.8 Larval Fat Body    135.7 Larval Salivary Gland    115.1 Larval Trachea    124.9 Larval Carcass    128.425 Adult Head    76.1 Adult Eye    105.975 Adult Brain    96.8 Adult Thoracic-Abdominal Ganglion    109.9 Adult Crop    126.8 Adult Midgut    136.1 Adult Hindgut    117.5 Adult Malpighian Tubules    137 Adult Fat Body    105 Adult Salivary Gland    206.8 Adult Heart    143.375 Adult VirginFemale Spermatheca    125.4 Adult InseminatedFemale Spermatheca    129.4 Adult Ovary    266.5 Adult Testis    275.1 Adult Male Accessory Gland    160.7 Adult Carcass    97.5 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    76.225 Larval Midgut    103.4 Larval Hindgut    100.4 Larval Malpighian Tubules    155.8 Larval Fat Body    135.7 Larval Salivary Gland    115.1 Larval Trachea    124.9 Larval Carcass    128.425 Adult Head    76.1 Adult Eye    105.975 Adult Brain    96.8 Adult Thoracic-Abdominal Ganglion    109.9 Adult Crop    126.8 Adult Midgut    136.1 Adult Hindgut    117.5 Adult Malpighian Tubules    137 Adult Fat Body    105 Adult Salivary Gland    206.8 Adult Heart    143.375 Adult VirginFemale Spermatheca    125.4 Adult InseminatedFemale Spermatheca    129.4 Adult Ovary    266.5 Adult Testis    275.1 Adult Male Accessory Gland    160.7 Adult Carcass    97.5 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    76.225 Larval Midgut    103.4 Larval Hindgut    100.4 Larval Malpighian Tubules    155.8 Larval Fat Body    135.7 Larval Salivary Gland    115.1 Larval Trachea    124.9 Larval Carcass    128.425 Adult Head    76.1 Adult Eye    105.975 Adult Brain    96.8 Adult Thoracic-Abdominal Ganglion    109.9 Adult Crop    126.8 Adult Midgut    136.1 Adult Hindgut    117.5 Adult Malpighian Tubules    137 Adult Fat Body    105 Adult Salivary Gland    206.8 Adult Heart    143.375 Adult VirginFemale Spermatheca    125.4 Adult InseminatedFemale Spermatheca    129.4 Adult Ovary    266.5 Adult Testis    275.1 Adult Male Accessory Gland    160.7 Adult Carcass    97.5 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    76.225 Larval Midgut    103.4 Larval Hindgut    100.4 Larval Malpighian Tubules    155.8 Larval Fat Body    135.7 Larval Salivary Gland    115.1 Larval Trachea    124.9 Larval Carcass    128.425 Adult Head    76.1 Adult Eye    105.975 Adult Brain    96.8 Adult Thoracic-Abdominal Ganglion    109.9 Adult Crop    126.8 Adult Midgut    136.1 Adult Hindgut    117.5 Adult Malpighian Tubules    137 Adult Fat Body    105 Adult Salivary Gland    206.8 Adult Heart    143.375 Adult VirginFemale Spermatheca    125.4 Adult InseminatedFemale Spermatheca    129.4 Adult Ovary    266.5 Adult Testis    275.1 Adult Male Accessory Gland    160.7 Adult Carcass    97.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0086362 expression level Tissue   Expression Level Larval Central Nervous System    76.225 Larval Midgut    103.4 Larval Hindgut    100.4 Larval Malpighian Tubules    155.8 Larval Fat Body    135.7 Larval Salivary Gland    115.1 Larval Trachea    124.9 Larval Carcass    128.425 Adult Head    76.1 Adult Eye    105.975 Adult Brain    96.8 Adult Thoracic-Abdominal Ganglion    109.9 Adult Crop    126.8 Adult Midgut    136.1 Adult Hindgut    117.5 Adult Malpighian Tubules    137 Adult Fat Body    105 Adult Salivary Gland    206.8 Adult Heart    143.375 Adult VirginFemale Spermatheca    125.4 Adult InseminatedFemale Spermatheca    129.4 Adult Ovary    266.5 Adult Testis    275.1 Adult Male Accessory Gland    160.7 Adult Carcass    97.5 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    76.225 Larval Midgut    103.4 Larval Hindgut    100.4 Larval Malpighian Tubules    155.8 Larval Fat Body    135.7 Larval Salivary Gland    115.1 Larval Trachea    124.9 Larval Carcass    128.425 Adult Head    76.1 Adult Eye    105.975 Adult Brain    96.8 Adult Thoracic-Abdominal Ganglion    109.9 Adult Crop    126.8 Adult Midgut    136.1 Adult Hindgut    117.5 Adult Malpighian Tubules    137 Adult Fat Body    105 Adult Salivary Gland    206.8 Adult Heart    143.375 Adult VirginFemale Spermatheca    125.4 Adult InseminatedFemale Spermatheca    129.4 Adult Ovary    266.5 Adult Testis    275.1 Adult Male Accessory Gland    160.7 Adult Carcass    97.5 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    76.225 Larval Midgut    103.4 Larval Hindgut    100.4 Larval Malpighian Tubules    155.8 Larval Fat Body    135.7 Larval Salivary Gland    115.1 Larval Trachea    124.9 Larval Carcass    128.425 Adult Head    76.1 Adult Eye    105.975 Adult Brain    96.8 Adult Thoracic-Abdominal Ganglion    109.9 Adult Crop    126.8 Adult Midgut    136.1 Adult Hindgut    117.5 Adult Malpighian Tubules    137 Adult Fat Body    105 Adult Salivary Gland    206.8 Adult Heart    143.375 Adult VirginFemale Spermatheca    125.4 Adult InseminatedFemale Spermatheca    129.4 Adult Ovary    266.5 Adult Testis    275.1 Adult Male Accessory Gland    160.7 Adult Carcass    97.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    76.225 Larval Midgut    103.4 Larval Hindgut    100.4 Larval Malpighian Tubules    155.8 Larval Fat Body    135.7 Larval Salivary Gland    115.1 Larval Trachea    124.9 Larval Carcass    128.425 Adult Head    76.1 Adult Eye    105.975 Adult Brain    96.8 Adult Thoracic-Abdominal Ganglion    109.9 Adult Crop    126.8 Adult Midgut    136.1 Adult Hindgut    117.5 Adult Malpighian Tubules    137 Adult Fat Body    105 Adult Salivary Gland    206.8 Adult Heart    143.375 Adult VirginFemale Spermatheca    125.4 Adult InseminatedFemale Spermatheca    129.4 Adult Ovary    266.5 Adult Testis    275.1 Adult Male Accessory Gland    160.7 Adult Carcass    97.5 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    76.1   NA  Eye    105.975   NA  Brain    96.8   76.225  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    109.9   NA  Crop    126.8   103.4  Midgut    136.1   100.4  Hindgut    117.5   155.8  Malpighian Tubules    137   135.7  Fat Body    105   115.1  Salivary Gland    206.8   NA  Heart    143.375   124.9  Trachea    NA   NA  VirginFemale Spermatheca    125.4   NA  InseminatedFemale Spermatheca    129.4   NA  Ovary    266.5   NA  Testis    275.1   NA  Male Accessory Gland    160.7   128.425  Carcass    97.5 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_01
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0086362 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG12114-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal (with Df(3R)A177der21) spn-F2 lethal | maternal effect spn-F1 lethal | recessive spn-F2 viable, with Scer\GAL4pnr-MD237 spn-FGD7489 Sterility Allele female sterile spn-F2 female sterile (with Df(3R)BSC793) spn-F1 semi-fertile spn-F1 Phenotype manifest in Allele actin tubule spn-Funspecified dorsal appendage spn-F2 spn-F1 dorsal appendage (with Df(3R)tll-e) spn-F2 spn-F1 egg spn-F1 egg (with Df(3R)tll-e) spn-F2 spn-F1 embryonic abdominal segment spn-F1 embryonic head spn-F1 macrochaeta spn-F2 spn-Funspecified macrochaeta, with Scer\GAL4neur-GAL4-A101 spn-F1 macrochaeta, with Scer\GAL4neur-P72 spn-FGD7489 microtubule cytoskeleton spn-Funspecified oocyte nucleus spn-Funspecified scutellar bristle, with Scer\GAL4neur-GAL4-A101 spn-F1
Classical Alleles ( 3 )
For All Classical Alleles Show
Allele of spn-F Class Mutagen Stocks Known lesion spn-F1 loss of function allele ethyl methanesulfonate 1 Yes spn-F2 loss of function allele Delta2-3 0 Yes spn-Funspecified 0 --
Alleles Carried on Transgenic Constructs ( 8 )
For All Alleles Carried on Transgenic Constructs Show
Allele of spn-F Class Mutagen Stocks Known lesion spn-FGD7489 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes spn-FKK103792 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes spn-FScer\UAS.cAa 0 Yes spn-FScer\UAS.P\T.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes spn-FScer\UAS.P\T.T:Disc\RFP-mCherry in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes spn-FT:Avic\GFP in vitro construct - genomic fragment in vitro construct - coding region fusion 0 Yes spn-FΔC.Scer\UAS.P\T.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes spn-FΔN.Scer\UAS.P\T.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)A177der21 (Deak et al., 1997, Abdu et al., 2006) Df(3R)BSC793 (Christensen et al., 2009.5.6) Df(3R)Exel6218 (Ryder, 2004.4.26)
Not disrupted in	Df(3R)A177der20 (Abdu et al., 2006)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD7489} NA P{KK103792} NA P{UASp-EGFP-spn-F} NA P{UASp-EGFP-spn-F-ΔC} NA P{UASp-EGFP-spn-F-ΔN} NA P{UASp-spn-F.mCherry} NA P{UAS-spn-F.A} NA characterization construct P{GFP-spn-F} NA
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 5 unique terms )
Terms Based on Experimental Evidence ( 5 terms )
Molecular Function
CV term Evidence References
minus-end-directed microtubule motor activity inferred from direct assay (Abdu et al., 2006)
Biological Process
CV term Evidence References
chaeta morphogenesis inferred from mutant phenotype (Abdu et al., 2006, Bitan et al., 2010, Dubin-Bar et al., 2011) oocyte axis specification inferred from mutant phenotype (Abdu et al., 2006) oocyte microtubule cytoskeleton organization inferred from direct assay (Abdu et al., 2006) regulation of cytoskeleton organization inferred from physical interaction with ik2 (Dubin-Bar et al., 2008)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 0 terms )
Sequence Ontology: Class of Gene
	gene   nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions spn-F allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 68545 DroID - A comprehensive database of gene and protein interactions. • FBgn0086362 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43700
Orthologs
Genome-wide drosophilid orthologs
Curated drosophilid orthologs	Dpse\GA11408
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL007749-PA Aedes aegypti (mosquito) AAEL014720-PA Aedes aegypti (mosquito) AGAP000758-PA Anopheles gambiae (mosquito) BGIBMGA012563-PA Bombyx mori (silk worm) CPIJ014044 Culex pipens (mosquito) XP_001602274.1 Nasonia vitripennis (wasp) PHUM008079-PA Pediculus humanus (human body louse) XP_973387.1 Tribolium castaneum (red flour beetle) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB16500 NV18790 LH12908 FBgn0228757 FBgn0212881 FBgn0210680 FBgn0192992 FBgn0167861 JGI_V11_228160 FBgn0071462 FBgn0151342 FBgn0146336 FBgn0125478 FBgn0104094 FBgn0094794 CPIJ014044 BGIBMGA012563 ACYPI50307 ACYPI32970 GB17853 AGAP000758 AAEL014720 AAEL007749 PHUM259910 GLEAN_00407
Stocks & Reagents
Stocks Listed in FlyBase ( 3 )
Bloomington	3325 Dp(1;Y)BS; ru1 st1 e1 spn-F1 ca1/TM3, Sb1
VDRC	v107850 P{KK103792}VIE-260B v17015 w1118; P{GD7489}v17015/TM3
Genomic Clones ( 1 )
	BACR30B13 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 53 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD01470
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT03445 LD11778 LD18176 LD23422 LD31987 RH02776 RH03127 RH17002 RH21962 RH27909 RH28752 RH31665 RH34389 RH36407 RH36482 RH53031 RH55633 RH60230 RH71740 SD26612 SD28251
Other clones	159142 277236 569464 EC23614 EC29914 EK164432 EK262418 EK304041 EN14934 IP12555 RP004032640 RP004035805 RP004085428 RP004096820 RP004118438 RP004212435 RP004230296 RP004259046 RP004266224 RP004266935 RP004380769 RP004407655 RP004438697 RP004494508 RP004497062 RP004498528 RP004508629 RP004514782 RP004515292 RP004521600 RP004527639
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0086362 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43700 252695
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: spn-F l(3)S113003b CG12114 (Abdu et al., 2006)
Additional comments
Other Comments
	Flies double mutant for mei-W68 or lok and spn-F exhibit neither ventralisation nor the defects in appearance of the DNA within the oocyte nuclei of spn-F, indicating suppression of the spn-F phenotype. (Abdu et al., 2006)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_143601 RefSeq (Proteins) • NP_651858 DDBJ / EMBL / GenBank • AA816416 AAF57139 AAL28594 AW941475 AY061046 Entrez Gene - A searchable database of RefSeq genes. • 43700 UniProtKB/TrEMBL • Q9V9Y9
Other Crossreferences
Linkouts
	BioGRID - A database of protein and genetic interactions • 68545 DroID - A comprehensive database of gene and protein interactions. • FBgn0086362 DRSC - Results from RNAi screens. • FBgn0086362 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0086362 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG12114-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0086362 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43700 252695 InParanoid A subset of ortholog calls from InParanoid. • AAEL007749-PA Aedes aegypti (mosquito) AAEL014720-PA Aedes aegypti (mosquito) AGAP000758-PA Anopheles gambiae (mosquito) BGIBMGA012563-PA Bombyx mori (silk worm) CPIJ014044 Culex pipens (mosquito) XP_001602274.1 Nasonia vitripennis (wasp) PHUM008079-PA Pediculus humanus (human body louse) XP_973387.1 Tribolium castaneum (red flour beetle) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43700 modMine - Data generated by the modENCODE project. • FBgn0086362 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB16500 NV18790 LH12908 FBgn0228757 FBgn0212881 FBgn0210680 FBgn0192992 FBgn0167861 JGI_V11_228160 FBgn0071462 FBgn0151342 FBgn0146336 FBgn0125478 FBgn0104094 FBgn0094794 CPIJ014044 BGIBMGA012563 ACYPI50307 ACYPI32970 GB17853 AGAP000758 AAEL014720 AAEL007749 PHUM259910 GLEAN_00407
Synonyms & Secondary IDs ( 9 )
Reported As
Symbol Synonym	1130/03 (Deak et al., 1997) CG12114 (Abdu et al., 2006, Keleman et al., 2009.8.5) l(3)S113003b   spn-F (Abdu et al., 2006, Bergmann, 2006, Dubin-Bar and Abdu, 2007, Dubin-Bar et al., 2008, Christensen et al., 2009.5.6, Bitan et al., 2010, Dubin-Bar et al., 2011) Spn-F (Dubin-Bar and Abdu, 2007, Gervais et al., 2008)
Name Synonym	CG12114   lethal (3) S113003b   spindle F   Spindle-F (Dubin-Bar and Abdu, 2007, Dubin-Bar et al., 2008) spindle-F (Bitan et al., 2010) spn-F (Abdu et al., 2006)
Secondary FlyBase IDs
	FBgn0003484 FBgn0039837 FBgn0060468
References ( 24 )
Generate a list of	All references relating to this gene ( 24 )
List References by type	Research paper  ( 7 ) Supplementary material  ( 3 ) Review  ( 1 ) Personal communication to FlyBase  ( 4 ) Abstract  ( 4 ) FlyBase analysis  ( 1 ) Stock list  ( 1 ) Book  ( 1 ) Computer file  ( 1 ) Automatic genome annotation  ( 1 )
Recent research papers ( 2 )
	Dubin-Bar et al., 2011, Development 138(21): 4661--4671 Drosophila javelin-like encodes a novel microtubule-associated protein and is required for mRNA localization during oogenesis. [FBrf0216406] Bitan et al., 2010, Mol. Cell. Biol. 30(2): 496--507 Asymmetric microtubule function is an essential requirement for polarized organization of the Drosophila bristle. [FBrf0209571] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...