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General Information
Symbol
Dmel\lap
Species
D. melanogaster
Name
like-AP180
Annotation Symbol
CG2520
Feature Type
FlyBase ID
FBgn0086372
Gene Model Status
Stock Availability
Gene Snapshot
like-AP180 (lap) encodes a cytosolic protein that acts as a clathrin adaptor to promote clathrin-coated vesicle formation and restrict coated vesicle size. At synapses it regulates synaptic vesicle size as well as the efficacy of synaptic vesicle protein retrieval. [Date last reviewed: 2019-03-14]
Also Known As

AP180, l(3)neo34, EP3060, dAP-3

Key Links
Genomic Location
Cytogenetic map
Sequence location
3R:7,186,994..7,200,941 [+]
Recombination map

3-48

RefSeq locus
NT_033777 REGION:7186994..7200941
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Protein Family (UniProt)
Belongs to the PICALM/SNAP91 family. (Q9VI75)
Summaries
Protein Function (UniProtKB)
Assembly protein recruiting clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction.
(UniProt, Q9VI75)
Gene Model and Products
Number of Transcripts
9
Number of Unique Polypeptides
9

Please see the GBrowse view of Dmel\lap or the JBrowse view of Dmel\lap for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.47

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Multiphase exon postulated: exon reading frame differs in alternative transcripts; overlap >20aa.

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0081572
4341
468
FBtr0302183
5126
758
FBtr0302184
5216
788
FBtr0305153
2984
572
FBtr0305154
3249
685
FBtr0305155
4871
673
FBtr0334886
4964
704
FBtr0334887
5180
776
FBtr0334888
4886
678
Additional Transcript Data and Comments
Reported size (kB)

4.2 (northern blot)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0081091
49.9
468
6.12
FBpp0291393
80.9
758
7.20
FBpp0291394
84.2
788
7.28
FBpp0293683
61.7
572
6.68
FBpp0293684
72.8
685
7.46
FBpp0293685
71.8
673
7.70
FBpp0306907
75.0
704
7.93
FBpp0306908
83.2
776
7.49
FBpp0306909
72.4
678
8.15
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Binds clathrin and phosphatidylinositol 4,5-bisphosphate.

(UniProt, Q9VI75)
Crossreferences
PDB - An information portal to biological macromolecular structures
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\lap using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Gene Ontology (24 terms)
Molecular Function (5 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000536700
(assigned by GO_Central )
inferred from sequence or structural similarity with UniProtKB:O55012
(assigned by UniProt )
inferred from sequence or structural similarity with UniProtKB:Q13492
(assigned by UniProt )
non-traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN000536700
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000536700
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000536700
(assigned by GO_Central )
Biological Process (13 terms)
Terms Based on Experimental Evidence (7 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from mutant phenotype
inferred from physical interaction with FLYBASE:Csp; FB:FBgn0004179
inferred from physical interaction with FLYBASE:Syt1; FB:FBgn0004242
inferred from physical interaction with FLYBASE:nSyb; FB:FBgn0013342
inferred from physical interaction with FLYBASE:VGlut; FB:FBgn0031424
Terms Based on Predictions or Assertions (7 terms)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR014712
(assigned by InterPro )
inferred from biological aspect of ancestor with PANTHER:PTN000536700
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000536700
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001119474
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001119474
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000536700
(assigned by GO_Central )
Cellular Component (6 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from direct assay
Terms Based on Predictions or Assertions (6 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000536700
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000536700
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001119474
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000536700
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001119474
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001119474
(assigned by GO_Central )
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

lap transcripts are expressed mainly in the central and peripheral nervous systems and in garland cells in embryos.

Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

lap protein is enriched at synaptic boutons of neuromuscular junctions in larvae. lap protein and Clc protein co-localize at the synaptic boutons. The level of Clc protein is significantly reduced in lap mutants.

Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\lap in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 13 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 10 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of lap
Transgenic constructs containing regulatory region of lap
Deletions and Duplications ( 7 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
14 of 15
Yes
Yes
 
11 of 15
No
Yes
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
14 of 15
Yes
Yes
10 of 15
No
Yes
Rattus norvegicus (Norway rat) (2)
10 of 13
Yes
Yes
9 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (3)
7 of 12
Yes
Yes
5 of 12
No
Yes
1 of 12
No
Yes
Danio rerio (Zebrafish) (6)
10 of 15
Yes
Yes
10 of 15
Yes
Yes
8 of 15
No
Yes
7 of 15
No
Yes
3 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
13 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (18)
6 of 9
Yes
Yes
6 of 9
Yes
Yes
5 of 9
No
Yes
5 of 9
No
Yes
5 of 9
No
Yes
5 of 9
No
Yes
5 of 9
No
Yes
3 of 9
No
Yes
3 of 9
No
Yes
3 of 9
No
Yes
1 of 9
No
Yes
1 of 9
No
Yes
1 of 9
No
Yes
1 of 9
No
Yes
1 of 9
No
Yes
1 of 9
No
Yes
1 of 9
No
Yes
1 of 9
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (2)
14 of 15
Yes
Yes
13 of 15
No
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
8 of 12
Yes
Yes
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091904GN )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091505YG )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Musca domestica
House fly
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W05XQ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X05TC )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0810 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 3 )
Potential Models Based on Orthology ( 1 )
Modifiers Based on Experimental Evidence ( 0 )
Allele
Disease
Interaction
References
Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Interactions Browser

Please see the Physical Interaction reports below for full details
protein-protein
Physical Interaction
Assay
References
RNA-protein
Physical Interaction
Assay
References
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement

Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Subunit Structure (UniProtKB)
Binds clathrin and phosphatidylinositol 4,5-bisphosphate.
(UniProt, Q9VI75 )
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Signaling Pathways (FlyBase)
Metabolic Pathways
External Data
Linkouts
Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
Genomic Location and Detailed Mapping Data
Chromosome (arm)
3R
Recombination map

3-48

Cytogenetic map
Sequence location
3R:7,186,994..7,200,941 [+]
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
84D1-84D2
Limits computationally determined from genome sequence between P{EP}EP3060EP3060 and P{PZ}grn05930
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
Determined by comparing Celera genomic sequence with sequence from BDGP BAC and P1 clones.
84D1-84D14
(determined by in situ hybridisation)
84C-84D
(determined by in situ hybridisation)
84D-84D
(determined by in situ hybridisation)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (12)
Genomic Clones (19)
 

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (74)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences
BDGP DGC clones
Other clones
Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)
RNAi and Array Information
Linkouts
DRSC - Results frm RNAi screens
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
Antibody Information
Laboratory Generated Antibodies
 
Commercially Available Antibodies
 
Other Information
Relationship to Other Genes
Source for database identify of

Source for identity of: lap CG2520

Source for database merge of

Source for merge of: lap EP3060

Source for merge of: lap BcDNA:GM10787

Additional comments

FlyBase curator comment: "EP3060" overexpression phenotype stated to be due to its effect on EST RE52393 (which corresponds to CG10055) but orientation of the P{EP} element suggests otherwise.

Source for merge of lap BcDNA:GM10787 was a shared cDNA ( date:020730 ).

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

A mutation in lap severely impairs the efficiency of synaptic vesicle endocytosis.

Origin and Etymology
Discoverer

A. Spradling.

Etymology
Identification
External Crossreferences and Linkouts ( 77 )
Sequence Crossreferences
NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
Other crossreferences
Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
KEGG Genes - Molecular building blocks of life in the genomic space.
modMine - A data warehouse for the modENCODE project
PDB - An information portal to biological macromolecular structures
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
DRSC - Results frm RNAi screens
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
FlyMine - An integrated database for Drosophila genomics
InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
MIST (protein-protein) - An integrated Molecular Interaction Database
Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
Synonyms and Secondary IDs (17)
Reported As
Symbol Synonym
BcDNA:GM10787
EP3060
lap/AP180
Secondary FlyBase IDs
  • FBgn0026210
  • FBgn0062670
  • FBgn0011467
  • FBgn0026062
  • FBgn0047251
Datasets (0)
Study focus (0)
Experimental Role
Project
Project Type
Title
References (110)