Gene Dmel\brp

(BDGP Project Members, 2000-, Wagh et al., 2006)

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

t-SNARE (IPR010989)

Prefoldin (IPR009053)

Helix-loop-helix DNA-binding (IPR011598)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Maps to

Does NOT map to

Identified with

AT09405

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

larval neuromuscular junction

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable, with Scer\GAL4^elav-C155

brp^{dsRNA.C.Scer\UAS}

viable, with Scer\GAL4^GMR.PF

brp^{dsRNA.C.Scer\UAS}

lethal | larval stage (with Df(2R)BSC29)

brp⁶⁹

Other Phenotypes

Allele

locomotor behavior defective, with Scer\GAL4^elav-C155

flight behavior defective, with Scer\GAL4^elav-C155

brp^{dsRNA.C.Scer\UAS}

neurophysiology defective (with Df(2R)BSC29)

brp⁶⁹

neurophysiology defective, with Scer\GAL4^elav-C155

brp^{dsRNA.C.Scer\UAS}

neuroanatomy defective (with Df(2R)BSC29)

brp⁶⁹

Phenotype manifest in

Allele

brp⁶⁹

t-bar

brp⁶⁹

t-bar, with Scer\GAL4^GMR.PF

brp^{dsRNA.C.Scer\UAS}

t-bar, with Scer\GAL4^elav-C155

brp^{dsRNA.C.Scer\UAS}

Classical Alleles ( 1 )

For All Classical Alleles Show

Allele of brp	Class	Mutagen	Stocks	Known lesion
brp⁶⁹		Δ2-3	0	Yes

Alleles Carried on Transgenic Constructs ( 8 )

For All Alleles Carried on Transgenic Constructs Show

Allele of brp	Mutagen	Stocks	Known lesion
brp^GD10045	in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
brp^GD10046	in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
brp^GD7874	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
brp^GD7875	in vitro construct \| RNAi in vitro construct \| regulatory fusion	0	Yes
brp^{Scer\UAS.T:Avic\GFP}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	0	Yes
brp^Scer\UAS.cWa	in vitro construct \| regulatory fusion	0	Yes
brp^{dsRNA.B.Scer\UAS}	in vitro construct \| regulatory fusion in vitro construct \| RNAi	0	Yes
brp^{dsRNA.C.Scer\UAS}	in vitro construct \| regulatory fusion in vitro construct \| RNAi	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(2R)w45-31n

In(2R)w45-67n

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

Insertions

Type of insertions

Name

Expression data

Related Comments

Please look at the allele reports for the complete phenotype data

brp mutant neuromuscular synapses suffer from active zone membrane defects, a complete loss of presynaptic dense bodies and severely depressed evoked vesicle release. Presynaptic Ca2+-channels become diffusely distributed in brp mutants.

(Sigrist et al., 2006)

RNAi knockdown flies show synaptic defects, impairment of walking behaviour and unstable flight.

(Nieratschker, 2005)

Gene Ontology: Function, Process & Cellular Component ( 12 )

Molecular Function

CV term

Evidence

References

calcium channel activity

inferred from mutant phenotype

(Sigrist et al., 2006)

protein binding

inferred from electronic annotation with InterPro:IPR010989

transcription regulator activity

inferred from electronic annotation with InterPro:IPR011598

adult locomotory behavior

Biological Process

CV term

Evidence

References

inferred from mutant phenotype

maintenance of presynaptic active zone structure

inferred from mutant phenotype

inferred from mutant phenotype

neurotransmitter secretion

inferred from mutant phenotype

regulation of synaptic plasticity

inferred from mutant phenotype

regulation of transcription

inferred from electronic annotation with InterPro:IPR011598

vesicle-mediated transport

inferred from electronic annotation with InterPro:IPR010989

Cellular Component

CV term

Evidence

References

membrane

inferred from electronic annotation with InterPro:IPR010989

nucleus

inferred from electronic annotation with InterPro:IPR011598

presynaptic active zone

inferred from direct assay

inferred from direct assay

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

brp allele

Gene

References

External Data

Orthologs

Genome-wide drosophilid orthologs

Dana\GF12472

Dere\GG24080

Dgri\GH19927

Dmoj\GI19884

Dper\GL15294

Dpse\GA11918

Dsec\GM21130

Dsim\GD15383

Dvir\GJ15053

Dwil\GK21819

Dyak\GE19283

Curated drosophilid orthologs

Dpse\GA11918

Dpse\GA11919

Dpse\GA15767

Dpse\GA15768

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 4 )

VDRC

v38746

w¹¹¹⁸; P{GD7874}v38746

v38748

w¹¹¹⁸; P{GD7874}v38748

v21135

w¹¹¹⁸; P{GD10046}v21135

v21134

w¹¹¹⁸; P{GD10045}v21134/TM3

Genomic Clones ( 2 )

cDNA Clones ( 16 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

IP11737

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

(Marrus and DiAntonio, 2004)

Antibody Information

monoclonal

Other Information

Discoverer

Etymology

Named "bruchpilot" as mutants for this gene display unstable flight and quickly crash to the ground.

Etymology: 'bruchpilot' is German for 'crash pilot'.

(Nieratschker, 2005)

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: brp Mabnc82

Source for merge of: brp CG12932

Source for merge of: brp CG12933 CG30336 CG30337

(Sigrist, 2006.2.28)

Additional comments

Annotations CG34146 and CG12932 merged as CG42344 in release 5.9 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Source for split of: CG1931 Annotation CG1931 split into CG30337 and CG30336 in release 3 of the genome annotation.

Annotations CG12933, CG30336 and CG30337 merged as CG34146 (which corresponds to brp) in release 5.1 of the genome annotation.

(FlyBase Genome Annotators, 2006)

Comments About Role

brp plays a role in promoting calcium channel clustering at the active zone.

(Wagh et al., 2006, Kittel et al., 2006, Kittel et al., 2006)

Comments About Molecular Function

Encodes a protein which is expressed in the neuropil structures of the adult brain.

(Laissue et al., 1999)

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

DDBJ / EMBL / GenBank

•

UniProtKB/TrEMBL

•

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

t-SNARE (IPR010989)

Prefoldin (IPR009053)

Helix-loop-helix DNA-binding (IPR011598)

Linkouts

Synonyms & Secondary IDs ( 19 )

Reported As

Symbol Synonym

BRP

brp

Cast

(Buchner, 2004.10.12)

CG1931

(Anholt and Mackay, 2001, )

CG12932

(FlyBase Genome Annotators, 2006)

CG12933

CG30336

CG30337

(Giot et al., 2003, )

CG34146

CG42344

(Komiyama et al., 2004, Heimbeck et al., 1999)

mAb nc82

MAbnc82

(Ashraf et al., 2006)

Mabnc82

nc82

(Benton et al., 2007, Liu et al., 2007, Komiyama, 2007, Kreher et al., 2005, Goldman et al., 2005, Berdnik et al., 2006, Ang et al., 2006, Ataman et al., 2006, Dudu et al., 2006, Dickman, 2006, Dickman et al., 2006, Longair et al., 2007, Hiesinger et al., 2005, Fishilevich et al., 2005, Liu et al., 2006, Yoshihara et al., 2005, Ramaekers et al., 2005, Pielage et al., 2005, Fishilevich and Vosshall, 2005, Larsson et al., 2004, Wang et al., 2004, Wucherpfennig et al., 2003, Marie et al., 2004, Yu et al., 2004, Hummel and Zipursky, 2004, Zhu and Luo, 2004, Jefferis et al., 2002, Zhu et al., 2003, Hummel et al., 2003, Pereanu and Hartenstein, 2003, Rein et al., 2002, Jefferis et al., 2001, Scott et al., 2001, Vosshall et al., 2000, Zhu et al., 2006, Mehta, 2005, Zhai et al., 2006, Kurtovic et al., 2007, Pielage et al., 2006, Jefferis et al., 2007, Wilson and Laurent, 2005, Wu et al., 2005, Dekker et al., 2006)

NC82

(Collins et al., 2006, Miller et al., 2005, Marrus and DiAntonio, 2004, Dulcis and Levine, 2005, Wu et al., 2005, Romero-Pozuelo et al., 2007)

Nc82

(Mahr and Aberle, 2006, Lichtneckert et al., 2007, Guerrero et al., 2005)

Name Synonym

bruchpilot

(Sigrist, 2006.2.28, Nieratschker, 2005, Sigrist et al., 2006, Wagh et al., 2006, Pielage et al., 2006)

Bruchpilot

(Pielage et al., 2006, Stowers and Isacoff, 2007)

CG12932

CG42344