FB2025_04 , released October 2, 2025
Gene: Dmel\RpS25
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General Information
Symbol
Dmel\RpS25
Species
D. melanogaster
Name
Ribosomal protein S25
Annotation Symbol
CG6684
Feature Type
protein_coding_gene
FlyBase ID
FBgn0086472
Gene Model Status
Current
Stock Availability
6 publicly available
Gene Summary
Contribute a Gene Snapshot for this gene.
All Summaries
Also Known As

M(3)S31, M(3)86D, M(3)S32

Key Links
Genomic Location
Cytogenetic map
86D8-86D8
Sequence location
Recombination map
3-50
RefSeq locus
NT_033777 REGION:11215028..11216287
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (4 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables structural constituent of ribosome
inferred from direct assay
(Anger et al., 2013)
inferred from high throughput direct assay
(Alonso and Santaren, 2006)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables structural constituent of ribosome
inferred from biological aspect of ancestor with PANTHER:PTN000982949
(GO Reference Genome Project, 2011-)
traceable author statement
(Marygold et al., 2007)
Biological Process (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in cytoplasmic translation
traceable author statement
(Marygold et al., 2007)
inferred by curator from GO:0022626
(Anger et al., 2013, Alonso and Santaren, 2006)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
located_in cytosolic ribosome
inferred from direct assay
(Anger et al., 2013)
inferred from high throughput direct assay
(Alonso and Santaren, 2006)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
part_of cytosolic small ribosomal subunit
inferred from biological aspect of ancestor with PANTHER:PTN000982949
(GO Reference Genome Project, 2011-)
traceable author statement
(Marygold et al., 2007)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the eukaryotic ribosomal protein eS25 family. (P48588)
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
CYTOPLASMIC SMALL RIBOSOMAL SUBUNIT -
Cytoplasmic ribosomes are found on the endoplasmic reticulum and in the cytosol. They translate all mRNAs produced from nuclear genes and perform the majority of cellular protein synthesis. Ribosomes are formed by two ribonucleoprotein subunits, large and small. The principle function of the small subunit is to bind mRNA. (Adapted from FBrf0205398.)
CYTOPLASMIC SMALL RIBOSOMAL SUBUNIT - TESTIS-SPECIFIC VARIANT -
Cytoplasmic ribosomes are found on the endoplasmic reticulum and in the cytosol. They translate all mRNAs produced from nuclear genes and perform the majority of cellular protein synthesis. Ribosomes are formed by two ribonucleoprotein subunits, large and small. The principle function of the small subunit is to bind mRNA. Tissue expression data from modENCODE has been used to infer the composition of the cytosolic small ribosomal subunit testis-specific variant. (Adapted from FBrf0205398.)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
M(3)86D
Fine-bristled Minute with medium viability. RK3.
M(3)S32
Medium Minute. Most flies thickset. RK3.
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\RpS25 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry P48588)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
PDB - An information portal to biological macromolecular structures
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.47

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
RpS25-RA
FBtr0082369
NM_079591
850
117
RpS25-RB
FBtr0082370
NM_169376
515
117
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
RpS25-PA
FBpp0081845
13.2
117
11.00
P48588
NP_524315
AAN13495
RpS25-PB
FBpp0081846
13.2
117
11.00
P48588
NP_731544
AAF54605
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

117 aa isoforms: RpS25-PA, RpS25-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
PDB - An information portal to biological macromolecular structures
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
UniProt
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\RpS25 using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.94

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism | ubiquitous

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 7 -- 8
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 9 -- 10
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 11 -- 12
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 13 -- 16
organism | ubiquitous
(Fisher et al., 2012)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
day 7 of adulthood -- day 49 of adulthood
adult heart
(Cammarato et al., 2011)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in cytosolic ribosome
inferred from direct assay
(Anger et al., 2013)
inferred from high throughput direct assay
(Alonso and Santaren, 2006)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\RpS25 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 4-6
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 2 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of RpS25
Transgenic constructs containing regulatory region of RpS25
Aberrations (Deficiencies and Duplications) ( 16 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (1)
Hsap\RPS25
14 of 14
Yes
Yes
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (4)
Rnor\Rps25
14 of 14
Yes
Yes
Rnor\RGD1563613
2 of 14
No
Yes
Rnor\RGD1564597
2 of 14
No
Yes
Rnor\LOC685085
1 of 14
No
Yes
Mus musculus (laboratory mouse) (1)
Mmus\Rps25
13 of 14
Yes
Yes
Xenopus tropicalis (Western clawed frog) (1)
Xtro\rps25
10 of 13
Yes
Yes
Danio rerio (Zebrafish) (1)
Drer\rps25
13 of 14
Yes
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\rps-25
14 of 14
Yes
Yes
Anopheles gambiae (African malaria mosquito) (2)
Agam\AgaP_AGAP004462
12 of 12
Yes
Yes
Agam\AgaP_AGAP005092
7 of 12
No
Yes
Arabidopsis thaliana (thale-cress) (4)
Atha\AT2G16360
12 of 13
Yes
Yes
Atha\AT2G21580
12 of 13
Yes
Yes
Atha\AT4G34555
12 of 13
Yes
Yes
Atha\AT4G39200
12 of 13
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (2)
Scer\RPS25A
12 of 13
Yes
Yes
Scer\RPS25B
12 of 13
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (2)
Spom\rps2501
11 of 12
Yes
Yes
Spom\rps2502
11 of 12
Yes
Yes
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:RpS25. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Allele
Disease
Evidence
References
Potential Models Based on Orthology ( 0 )
Human Ortholog
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 2 )
Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
RPS25; ribosomal protein S25
14 of 14
180465
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      Interaction Browsers
      View in FlyBase Interactions Browser   View in MIST tool (external link)
      Please see the Physical Interaction reports below for full details
      protein-protein
      Physical Interaction
      Assay
      References
      RpS25 - Rack1
      cosedimentation, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL10
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL10Ab
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL11
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL12
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL13
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL13A
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL14
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL15
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL17
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL18
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL18A
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL19
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL21
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL22
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL23
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL23A
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL24
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL26
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL27
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL27A
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL28
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL29
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL3
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL30
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL31
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL32
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL34a
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL35
      cosedimentation, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL35A
      cosedimentation, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL36
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL36A
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL37-1
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL37A
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL38
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL39
      cosedimentation, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL4
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL40
      cosedimentation, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL41
      cosedimentation, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL5
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL6
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL7
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL7A
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL8
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpL9
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpLP0
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpLP1
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpLP2
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpS10a
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpS11
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpS12
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpS13
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpS14a
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpS14b
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpS15
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpS15Aa
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpS16
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpS17
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpS18
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpS19a
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpS2
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpS20
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpS21
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpS23
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpS24
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpS26
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpS27
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpS27A
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpS28b
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpS29
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpS3
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpS30
      cosedimentation, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpS3A
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpS4
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpS5a
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpS6
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpS7
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpS8
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - RpS9
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - S6kII
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      RpS25 - eEF2
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - gig
      anti tag coimmunoprecipitation, Identification by mass spectrometry
      (Vinayagam et al., 2016)
      RpS25 - pix
      tandem affinity purification, molecular weight estimation by silver staining, Identification by mass spectrometry
      (Andersen and Leevers, 2007)
      RpS25 - sta
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS25 - vig2
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RNA-protein
      Physical Interaction
      Assay
      References
      RpS25 - 18SrRNA
      cosedimentation, electron microscopy
      (Anger et al., 2013)
      RpS25 - 28SrRNA
      cosedimentation, electron microscopy
      (Anger et al., 2013)
      RpS25 - 2SrRNA
      cosedimentation, electron microscopy
      (Anger et al., 2013)
      RpS25 - 5.8SrRNA
      cosedimentation, electron microscopy
      (Anger et al., 2013)
      RpS25 - 5SrRNA
      cosedimentation, electron microscopy
      (Anger et al., 2013)
      Summary of Genetic Interactions
      Interaction Browsers
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      External Data
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Pathways
      Class of Gene
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      3R
      Recombination map
      3-50
      Cytogenetic map
      86D8-86D8
      Sequence location
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      86D8-86D8
      Limits computationally determined from genome sequence between P{EP}EP3340EP3340 and P{PZ}tho1&P{PZ}l(3)0462905275
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      Experimentally Determined Recombination Data
      Location

      3-50

      (FlyBase, 2016)

      3-47.7

      (Comeron et al., 2012)

      3-

      3-50.0

      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (6)
      Genomic Clones (14)
       

      Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

      cDNA Clones (699)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequenced
      BDGP DGC clones
      Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
      Other clones
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      Antibody Information
      Laboratory Generated Antibodies
       

      polyclonal

      (Nishiyama et al., 2007)
      Commercially Available Antibodies
       
      Cell Line Information
      Publicly Available Cell Lines
       
        Other Stable Cell Lines
         
          Other Comments

          Likely Minute gene.

          (Marygold et al., 2007)

          A deletion that removes RpS25 and RpL3 but no other cytoplasmic ribosomal protein-encoding genes shows Minute phenotypes, whereas molecularly-defined mutations in RpL3 do not result in Minute phenotypes. This suggests that RpS25 is a Minute gene.

          (Marygold et al., 2007)

          The M(3)86D mutant phenotype is not suppressed by RpL32+t3.3.

          (Kongsuwan et al., 1985)

          One of a class of genes (see MIN record) that when present in one, rather than two, copies, produce a characteristic phenotype consisting of short slender bristles and delayed development.

          One of a class of genes (see MIN record) that when present in one, rather than two, copies, produce a characteristic phenotype consisting of short slender bristles and delayed development. Identified by virtue of Minute phenotype of Df(3R)M86D; This Minute erroneously designated M(3)S35 (Lindsley et al., 1972).

          Relationship to Other Genes
          Source for database merge of

          Source for merge of: RpS25 M(3)S32 M(3)86D

          (Cook, 2006.10.1)
          Additional comments

          "RpS25" likely corresponds to "M(3)86D".

          (Marygold et al., 2007)

          Mapping against deficiencies in 86D suggests M(3)86D may correspond to RpL3 and/or RpS25.

          (Roote, 2004.6.24)
          Nomenclature History
          Source for database identify of
          Nomenclature comments
          Etymology
          Synonyms and Secondary IDs (18)
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          External Crossreferences and Linkouts ( 49 )
          Sequence Crossreferences
          NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
          GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
          GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
          RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
          UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
          UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
          Other crossreferences
          AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
          BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
          DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
          EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
          FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
          FlyMine - An integrated database for Drosophila genomics
          InterPro - A database of protein families, domains and functional sites
          KEGG Genes - Molecular building blocks of life in the genomic space.
          MARRVEL_MODEL - MARRVEL (model organism gene)
          PDB - An information portal to biological macromolecular structures
          Linkouts
          BioGRID - A database of protein and genetic interactions.
          Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
          DroID - A comprehensive database of gene and protein interactions.
          DRSC - Results frm RNAi screens
          FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
          FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
          Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
          Flygut - An atlas of the Drosophila adult midgut
          FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
          iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
          KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
          MIST (protein-protein) - An integrated Molecular Interaction Database
          Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
          References (77)