FlyBase Gene Report: Dmel\cbs

General Information

Symbol

Dmel\cbs

Species

D. melanogaster

Name

centrosomin's beautiful sister

Annotation Symbol

CG4840

Feature Type

protein_coding_gene

FlyBase ID

FBgn0086757

Gene Model Status

Current

Stock Availability

4 publicly available

Gene Summary

centrosomin's beautiful sister (cbs) encodes a a Golgi protein required for the centrosome cycle. [Date last reviewed: 2019-07-11] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

dGolgin-97, dem

Key Links

Genomic Location

Cytogenetic map

50A9-50A9

Sequence location

2R:13,450,586..13,453,393 [+]

Recombination map

2-68

RefSeq locus

NT_033778 REGION:13450586..13453393

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (9 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables small GTPase binding

inferred from physical interaction with FLYBASE:Arl1; FB:FBgn0000115, FLYBASE:Rab19; FB:FBgn0015793, FLYBASE:Rab6; FB:FBgn0015797, FLYBASE:Rab30; FB:FBgn0031882

(Sinka et al., 2008)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in centrosome cycle

inferred from mutant phenotype

(Eisman et al., 2006)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in Golgi vesicle transport

inferred by curator from GO:0000138,GO:0031267

(Sinka et al., 2008)

Cellular Component (6 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

located_in chromatin

inferred from direct assay

(Eisman et al., 2006)

located_in cytoplasm

inferred from direct assay

(Eisman et al., 2006)

located_in Golgi apparatus

inferred from direct assay

(Torres et al., 2014)

located_in Golgi trans cisterna

inferred from direct assay

(Sinka et al., 2008)

located_in perinuclear region of cytoplasm

inferred from direct assay

(Eisman et al., 2006)

located_in spindle

inferred from direct assay

(Eisman et al., 2006)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in Golgi apparatus

inferred from biological aspect of ancestor with PANTHER:PTN001504455

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

GOLGINS

Protein Family (UniProt)

Protein Signatures (InterPro)

GRIP domain

Summaries

Gene Snapshot

centrosomin's beautiful sister (cbs) encodes a a Golgi protein required for the centrosome cycle. [Date last reviewed: 2019-07-11]

Gene Group (FlyBase)

GOLGINS -: Golgins are long, parallel coiled-coil homodimers important in regulating vesicle mediated transport and Golgi architecture. They are anchored to specific sites on the Golgi complex surface either by a C-terminal transmembrane domain or interacting with Arf/Arf-like GTPases via a GRIP/GRAB domain. Golgins are thought to act as tethering factors, interacting with vesicles by binding Rab GTPases and guiding them to the correct Golgi cisternae. (Adapted from PMID:21436057).

Summary (Interactive Fly)

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\cbs for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q7K4K7)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.41

Gene model reviewed during 5.49

Sequence Ontology: Class of Gene

gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

cbs-RA

FBtr0087666

NM_137031

2257

611

cbs-RB

FBtr0309886

NM_001259368

2233

603

cbs-RC

FBtr0309887

NM_001259369

2227

601

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

cbs-PA

FBpp0086786

70.8

611

4.97

FBpp0301620

69.9

603

4.93

FBpp0301621

69.7

601

4.93

A0A0B4K864

NP_001246298

AFH08052

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

GRIP domain

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\cbs using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

cbs_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that the Fly Cell Atlas project found the gene expressed in that cell type. Darker colors mean that more cells of that cell type express the gene:

low

high

Colorless tiles indicate that there is no scRNAseq data for the gene in that cell type.

Colored tiles in ribbon indicate that expression data (RNA and/or protein) has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

Colored tiles in the ribbon indicate the average RNA expression level of the gene at the indicated stages:

low

high

as determined by RNA-seq (RPKM) using whole organism samples modENCODE, Brown et al., 2014. For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in chromatin

inferred from direct assay

(Eisman et al., 2006)

located_in cytoplasm

inferred from direct assay

(Eisman et al., 2006)

located_in Golgi apparatus

inferred from direct assay

(Torres et al., 2014)

located_in Golgi trans cisterna

inferred from direct assay

(Sinka et al., 2008)

located_in perinuclear region of cytoplasm

inferred from direct assay

(Eisman et al., 2006)

located_in spindle

inferred from direct assay

(Eisman et al., 2006)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\cbs in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\cbs

View exonic expression by tissue for Dmel\cbs

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36492/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0086757

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0086757

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0086757

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4840

Flygut - An atlas of the Drosophila adult midgut

FBgn0086757

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 3 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 6 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of cbs

Transgenic constructs containing regulatory region of cbs

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die during embryonic stage | partially, with Scer\GAL4^VP16.nos.UTR

cbs^{GRIP.UASp.GFP}

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

cbs^GD11443

viable, with Scer\GAL4^Mef2.PR

cbs^GD11443

viable, with Scer\GAL4^pnr-MD237

cbs^GD11443

viable, with Scer\GAL4^tin.CΔ4

cbs^GD11443

Other Phenotypes

Allele

abnormal mitotic cell cycle | embryonic stage, with Scer\GAL4^VP16.nos.UTR

cbs^{GRIP.UASp.GFP}

some die during embryonic stage | partially, with Scer\GAL4^VP16.nos.UTR

cbs^{GRIP.UASp.GFP}

Phenotype manifest in

Allele

centrosome, with Scer\GAL4^VP16.nos.UTR

cbs^{dsRNA.Sym.UAS}

centrosome | embryonic stage 4, with Scer\GAL4^VP16.nos.UTR

cbs^{dsRNA.Sym.UAS}

centrosome | embryonic stage 5, with Scer\GAL4^VP16.nos.UTR

cbs^{dsRNA.Sym.UAS}

centrosome | increased number, with Scer\GAL4^VP16.nos.UTR

cbs^{GRIP.UASp.GFP}

embryo | embryonic stage 4, with Scer\GAL4^VP16.nos.UTR

cbs^{dsRNA.Sym.UAS}

embryo | embryonic stage 5, with Scer\GAL4^VP16.nos.UTR

cbs^{dsRNA.Sym.UAS}

nucleus | embryonic stage 4, with Scer\GAL4^VP16.nos.UTR

cbs^{dsRNA.Sym.UAS}

nucleus | embryonic stage 5, with Scer\GAL4^VP16.nos.UTR

cbs^{dsRNA.Sym.UAS}

spindle | embryonic stage 4, with Scer\GAL4^VP16.nos.UTR

cbs^{dsRNA.Sym.UAS}

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (2)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\GOLGA1

12 of 15

Yes

Hsap\CCDC186

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (2)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Golga1

11 of 15

Yes

Mmus\Ccdc186

1 of 15

Rattus norvegicus (Norway rat) (1)

Rnor\Golga1

11 of 13

Yes

Xenopus tropicalis (Western clawed frog) (1)

Xtro\golga1

10 of 12

Yes

Danio rerio (Zebrafish) (3)

Drer\golga1

12 of 15

Yes

Drer\ccdc186

1 of 15

Drer\si:ch211-286b5.4

1 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (7)

Cele\cccp-1

1 of 15

Yes

Cele\dnc-2

1 of 15

Yes

Cele\gcc-2

1 of 15

Yes

Cele\T01C3.3

1 of 15

Yes

Cele\T01G5.7

1 of 15

Yes

Cele\Y45F10B.8

1 of 15

Yes

Cele\Y45F10B.9

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (1)

Atha\AT5G44310

1 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\brl1

1 of 12

Yes

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (2)

Gene Symbol

Score

Source

Alignment

2 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

GOLGA1; golgin A1

12 of 15

602502

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

cbs - Arl1

two hybrid, pull down, western blot

(Sinka et al., 2008)

cbs - Dsor1

experimental knowledge based

(Guruharsha et al., 2011)

cbs - Nepl3

experimental knowledge based

(Guruharsha et al., 2011)

cbs - Rab19

pull down, western blot, two hybrid

(Sinka et al., 2008)

cbs - Rab30

two hybrid, pull down, western blot, peptide massfingerprinting

(Gillingham et al., 2014, Sinka et al., 2008)

cbs - Rab6

pull down, peptide massfingerprinting, western blot, two hybrid

(Gillingham et al., 2014, Sinka et al., 2008)

cbs - TER94

experimental knowledge based

(Guruharsha et al., 2011)

cbs - drl

experimental knowledge based

(Guruharsha et al., 2011)

cbs - gkt

experimental knowledge based, inferred by curator

(Guruharsha et al., 2011)

cbs - jar

pull down, anti tag western blot, peptide massfingerprinting

(Finan et al., 2011)

cbs - vret

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

62252

DroID - A comprehensive database of gene and protein interactions.

FBgn0086757

MIST (protein-protein) - An integrated Molecular Interaction Database

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0086757

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Retrograde transport at the Trans-Golgi-Network

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-68

Cytogenetic map

50A9-50A9

Sequence location

2R:13,450,586..13,453,393 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

50A9-50A9

Limits computationally determined from genome sequence between P{EP}Dp^EP358 and P{lacW}drk^k13809&P{EP}mip120^EP606

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-68

(FlyBase, 2016)

2-72.3

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

cbs is very tightly linked to cnn.

(Megraw et al., 1999)

Stocks and Reagents

Stocks (4)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

26992

y¹ w^*; P{EP}cbs^G2391

91515

y¹ w^*; TI{CRIMIC.TG4.0}cbs^{CR02345-TG4.0}

VDRC

v103509

P{KK112417}VIE-260B

v21959

w¹¹¹⁸; P{GD11443}v21959

Genomic Clones (22)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (14)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD35238

Other clones

DPiM_LD35238

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0086757

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0086757

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

36492

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Sinka et al., 2008)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: cbs CG4840

(Eisman et al., 2006)

Additional comments

Other Comments

cbs protein undergoes a dramatic relocalization during mitosis from the cytoplasm to an association with chromosomes from late prometaphase to early telophase.

(Eisman et al., 2006)

cbs appears to participate in microtubule organising centre function.

(Megraw et al., 1999)

Transcription start sites of cnn and cbs are separated by a mere 134bp. Overall orientation not stated: cnn- cbs+

(Megraw, 1999.3.3)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 43 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

36492

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q7K4K7

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q7K4K7

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0086757

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36492/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0086757

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

cbs_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0086757

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0086757

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4840

Flygut - An atlas of the Drosophila adult midgut

FBgn0086757

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0086757

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

36492

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC014288

InterPro - A database of protein families, domains and functional sites

GRIP domain

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG4840

MARRVEL_MODEL - MARRVEL (model organism gene)

36492

modMine - A data warehouse for the modENCODE project

FBgn0086757

Linkouts

BioGRID - A database of protein and genetic interactions.

62252

DroID - A comprehensive database of gene and protein interactions.

FBgn0086757

DRSC - Results frm RNAi screens

FBgn0086757

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0086757

FlyMine - An integrated database for Drosophila genomics

FBgn0086757

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (protein-protein) - An integrated Molecular Interaction Database

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Retrograde transport at the Trans-Golgi-Network

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

CG4840

(Mathew et al., 2011, Munro, 2011, Keleman et al., 2009.8.5, Sinka et al., 2008, Eisman et al., 2006, Yepiskoposyan et al., 2006)

Cbs

(Finan et al., 2011)

cbs

(Shilova et al., 2020, Gene Disruption Project members, 2018-, Bischof and FlyORF project members, 2014.6.20, Chen et al., 2014, Japanese National Institute of Genetics, 2012.5.21, Munro, 2011, Eisman et al., 2006)

dGolgin-97

(Torres et al., 2014, Sinka et al., 2008)

dGolgin97

(Cai et al., 2019)

dem

(Megraw et al., 1999, Megraw et al., 1998)

Name Synonyms

CG4840

Centrosomin's beautiful sister

(Finan et al., 2011)

Golgin 97

(Southon et al., 2010)

centrosomin's beautiful sister

(Eisman et al., 2006, Megraw, 1999.3.3)

dGolgin-97

(Sinka et al., 2008)

dementin

(Megraw et al., 1999)

Secondary FlyBase IDs

FBgn0026256
FBgn0033840

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

References (43)

The following external sites may use different assemblies or annotations than FlyBase.

Report Sections