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Gene Dmel\Pcmt

General Information
Symbol	Dmel\Pcmt	Species	D. melanogaster
Name	Protein-L-isoaspartate (D-aspartate) O-methyltransferase	Annotation symbol	CG2152
Feature type	protein_coding_gene	FlyBase ID	FBgn0086768
Gene Model Status	Current	Stock availability	12 publicly available
Also Known As	PIMT
Genomic Location
Chromosome (arm)	3R	Recombination map	3-
Cytogenetic map	101F-83B1	Sequence location	3R:1,378,545..1,380,033 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Protein-L-isoaspartate (D-aspartate) O-methyltransferase is referred to in FlyBase by the symbol Dmel\Pcmt (CG2152, FBgn0086768). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein-L-isoaspartate (D-aspartate) O-methyltransferase activity. It is reported to be involved in the biological process: protein repair; protein modification process. 7 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Protein-L-isoaspartate(D-aspartate) O-methyltransferase. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderately high expression. Peak expression observed within 00-12 hour embryonic stages, during late larval stages, at stages throughout the pupal period, in stages of adults of both sexes. Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult eye, larval trachea. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, adult female reproductive system, adult male reproductive system, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1626213_a_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Pcmt. Gene sequence location is 3R:1378545..1380033.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01	Polypeptides Pcmt-PB Sequence features BKN29391 HFA12363 Transcripts Pcmt-RB
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 101F-83B1   Left limit from in situ hybridisation (FBrf0085080) Right limit from in situ hybridisation (FBrf0085080); Limits computationally determined from genome sequence between P{PZ}Snr101319&P{PZ}Itp-r83A05616 and P{lacW}Atus1938
Experimentally Determined Cytological Location
Cytogenetic map Notes References 83A5-83A6     (O'Connor, 2000.1.4) 83A-83B     (O'Connor, 1995.12.21) 83A-83B   (determined by in situ hybridisation)   (O'Connor et al., 1997) 101F-102A   (determined by in situ hybridisation)   (Galus et al., 1995)
Experimentally Determined Recombination Data
Location	3- (O'Connor, 1995.12.21)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Pcmt for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.43
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Pcmt-RA FBtr0078758  NM_080508   948   226 Pcmt-RB FBtr0310546     993   203
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Pcmt-PA   FBpp0078405   24.6   226   7.23     NP_536756   AAF52012 Pcmt-PB   FBpp0302683   21.9   203   7.70
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Protein-L-isoaspartate(D-aspartate) O-methyltransferase (IPR000682)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014297 AAF52012   AI064650   AI107211   AI107913   AI109342   AI135034   AI238109   AI402997   AI403979   AI405223   AY070630 AAL48101   AY075527 AAL68334   BG637203   BI354257   BI358667   BI364426   BI368372   BI371990   BI486138   BI579760   BI580301   BI608906   BI610037   BT125930   CO280326   CO283233   CO313144   CO316568   CO317412   CO317858   EC062017   EC076806   EC078105   EC078172   EC079139   EC080468   EC084781   EC225592   EL871095   EL871096   EL877608   ES690359   ES690397   ES692309   EV582699   U37432 AAA80540   U43737 AAA86272
UniProtKB/Swiss-Prot	Q27869
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0086768    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0086768 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderately high expression. Peak expression observed within 00-12 hour embryonic stages, during late larval stages, at stages throughout the pupal period, in stages of adults of both sexes. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0086768 expression level Developmental Stage   Expression Level embryo 00-02hr    3500 embryo 02-04hr    5359 embryo 04-06hr    4797 embryo 06-08hr    4282 embryo 08-10hr    4085 embryo 10-12hr    4668 embryo 12-14hr    2652 embryo 14-16hr    2693 embryo 16-18hr    2150 embryo 18-20hr    1865 embryo 20-22hr    1856 embryo 22-24hr    1524 larva L1    1666 larva L2    1747 larva L3 12hr old    2430 larva L3 puffstage 1-2    4824 larva L3 puffstage 3-6    5145 larva L3 puffstage 7-9    5497 white prepupae new    4844 white prepupae 12hr    4516 white prepupae 24hr    5822 pupae 2d postWPP    4647 pupae 3d postWPP    3886 pupae 4d postWPP    5084 adult male 01day    5129 adult male 05day    6688 adult male 30day    5749 adult female 01day    4299 adult female 05day    3237 adult female 30day    3180 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (3500) embryo 02-04hr  (5359) embryo 04-06hr  (4797) embryo 06-08hr  (4282) embryo 08-10hr  (4085) embryo 10-12hr  (4668) embryo 12-14hr  (2652) embryo 14-16hr  (2693) embryo 16-18hr  (2150) embryo 18-20hr  (1865) embryo 20-22hr  (1856) embryo 22-24hr  (1524) larva L1  (1666) larva L2  (1747) larva L3 12hr old  (2430) larva L3 puffstage 1-2  (4824) larva L3 puffstage 3-6  (5145) larva L3 puffstage 7-9  (5497) white prepupae new  (4844) white prepupae 12hr  (4516) white prepupae 24hr  (5822) pupae 2d postWPP  (4647) pupae 3d postWPP  (3886) pupae 4d postWPP  (5084) adult male 01day  (5129) adult male 05day  (6688) adult male 30day  (5749) adult female 01day  (4299) adult female 05day  (3237) adult female 30day  (3180) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (3500) embryo 02-04hr  (5359) embryo 04-06hr  (4797) embryo 06-08hr  (4282) embryo 08-10hr  (4085) embryo 10-12hr  (4668) embryo 12-14hr  (2652) embryo 14-16hr  (2693) embryo 16-18hr  (2150) embryo 18-20hr  (1865) embryo 20-22hr  (1856) embryo 22-24hr  (1524) larva L1  (1666) larva L2  (1747) larva L3 12hr old  (2430) larva L3 puffstage 1-2  (4824) larva L3 puffstage 3-6  (5145) larva L3 puffstage 7-9  (5497) white prepupae new  (4844) white prepupae 12hr  (4516) white prepupae 24hr  (5822) pupae 2d postWPP  (4647) pupae 3d postWPP  (3886) pupae 4d postWPP  (5084) adult male 01day  (5129) adult male 05day  (6688) adult male 30day  (5749) adult female 01day  (4299) adult female 05day  (3237) adult female 30day  (3180) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    3500 embryo 02-04hr    5359 embryo 04-06hr    4797 embryo 06-08hr    4282 embryo 08-10hr    4085 embryo 10-12hr    4668 embryo 12-14hr    2652 embryo 14-16hr    2693 embryo 16-18hr    2150 embryo 18-20hr    1865 embryo 20-22hr    1856 embryo 22-24hr    1524 larva L1    1666 larva L2    1747 larva L3 12hr old    2430 larva L3 puffstage 1-2    4824 larva L3 puffstage 3-6    5145 larva L3 puffstage 7-9    5497 white prepupae new    4844 white prepupae 12hr    4516 white prepupae 24hr    5822 pupae 2d postWPP    4647 pupae 3d postWPP    3886 pupae 4d postWPP    5084 adult male 01day    5129 adult male 05day    6688 adult male 30day    5749 adult female 01day    4299 adult female 05day    3237 adult female 30day    3180 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    3500 embryo 02-04hr    5359 embryo 04-06hr    4797 embryo 06-08hr    4282 embryo 08-10hr    4085 embryo 10-12hr    4668 embryo 12-14hr    2652 embryo 14-16hr    2693 embryo 16-18hr    2150 embryo 18-20hr    1865 embryo 20-22hr    1856 embryo 22-24hr    1524 larva L1    1666 larva L2    1747 larva L3 12hr old    2430 larva L3 puffstage 1-2    4824 larva L3 puffstage 3-6    5145 larva L3 puffstage 7-9    5497 white prepupae new    4844 white prepupae 12hr    4516 white prepupae 24hr    5822 pupae 2d postWPP    4647 pupae 3d postWPP    3886 pupae 4d postWPP    5084 adult male 01day    5129 adult male 05day    6688 adult male 30day    5749 adult female 01day    4299 adult female 05day    3237 adult female 30day    3180 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0086768 expression level Developmental Stage   Expression Level embryo 00-02hr    3500 embryo 02-04hr    5359 embryo 04-06hr    4797 embryo 06-08hr    4282 embryo 08-10hr    4085 embryo 10-12hr    4668 embryo 12-14hr    2652 embryo 14-16hr    2693 embryo 16-18hr    2150 embryo 18-20hr    1865 embryo 20-22hr    1856 embryo 22-24hr    1524 larva L1    1666 larva L2    1747 larva L3 12hr old    2430 larva L3 puffstage 1-2    4824 larva L3 puffstage 3-6    5145 larva L3 puffstage 7-9    5497 white prepupae new    4844 white prepupae 12hr    4516 white prepupae 24hr    5822 pupae 2d postWPP    4647 pupae 3d postWPP    3886 pupae 4d postWPP    5084 adult male 01day    5129 adult male 05day    6688 adult male 30day    5749 adult female 01day    4299 adult female 05day    3237 adult female 30day    3180 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (3500) embryo 02-04hr  (5359) embryo 04-06hr  (4797) embryo 06-08hr  (4282) embryo 08-10hr  (4085) embryo 10-12hr  (4668) embryo 12-14hr  (2652) embryo 14-16hr  (2693) embryo 16-18hr  (2150) embryo 18-20hr  (1865) embryo 20-22hr  (1856) embryo 22-24hr  (1524) larva L1  (1666) larva L2  (1747) larva L3 12hr old  (2430) larva L3 puffstage 1-2  (4824) larva L3 puffstage 3-6  (5145) larva L3 puffstage 7-9  (5497) white prepupae new  (4844) white prepupae 12hr  (4516) white prepupae 24hr  (5822) pupae 2d postWPP  (4647) pupae 3d postWPP  (3886) pupae 4d postWPP  (5084) adult male 01day  (5129) adult male 05day  (6688) adult male 30day  (5749) adult female 01day  (4299) adult female 05day  (3237) adult female 30day  (3180) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (3500) embryo 02-04hr  (5359) embryo 04-06hr  (4797) embryo 06-08hr  (4282) embryo 08-10hr  (4085) embryo 10-12hr  (4668) embryo 12-14hr  (2652) embryo 14-16hr  (2693) embryo 16-18hr  2150 embryo 18-20hr  1865 embryo 20-22hr  1856 embryo 22-24hr  1524 larva L1  1666 larva L2  1747 larva L3 12hr old  2430 larva L3 puffstage 1-2  (4824) larva L3 puffstage 3-6  (5145) larva L3 puffstage 7-9  (5497) white prepupae new  (4844) white prepupae 12hr  (4516) white prepupae 24hr  (5822) pupae 2d postWPP  (4647) pupae 3d postWPP  (3886) pupae 4d postWPP  (5084) adult male 01day  (5129) adult male 05day  (6688) adult male 30day  (5749) adult female 01day  (4299) adult female 05day  (3237) adult female 30day  (3180) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    3500 embryo 02-04hr    5359 embryo 04-06hr    4797 embryo 06-08hr    4282 embryo 08-10hr    4085 embryo 10-12hr    4668 embryo 12-14hr    2652 embryo 14-16hr    2693 embryo 16-18hr    2150 embryo 18-20hr    1865 embryo 20-22hr    1856 embryo 22-24hr    1524 larva L1    1666 larva L2    1747 larva L3 12hr old    2430 larva L3 puffstage 1-2    4824 larva L3 puffstage 3-6    5145 larva L3 puffstage 7-9    5497 white prepupae new    4844 white prepupae 12hr    4516 white prepupae 24hr    5822 pupae 2d postWPP    4647 pupae 3d postWPP    3886 pupae 4d postWPP    5084 adult male 01day    5129 adult male 05day    6688 adult male 30day    5749 adult female 01day    4299 adult female 05day    3237 adult female 30day    3180 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    3500 embryo 02-04hr    5359 embryo 04-06hr    4797 embryo 06-08hr    4282 embryo 08-10hr    4085 embryo 10-12hr    4668 embryo 12-14hr    2652 embryo 14-16hr    2693 embryo 16-18hr    2150 embryo 18-20hr    1865 embryo 20-22hr    1856 embryo 22-24hr    1524 larva L1    1666 larva L2    1747 larva L3 12hr old    2430 larva L3 puffstage 1-2    4824 larva L3 puffstage 3-6    5145 larva L3 puffstage 7-9    5497 white prepupae new    4844 white prepupae 12hr    4516 white prepupae 24hr    5822 pupae 2d postWPP    4647 pupae 3d postWPP    3886 pupae 4d postWPP    5084 adult male 01day    5129 adult male 05day    6688 adult male 30day    5749 adult female 01day    4299 adult female 05day    3237 adult female 30day    3180 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0086768    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0086768 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult eye, larval trachea. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, adult female reproductive system, adult male reproductive system, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0086768 expression level Tissue   Expression Level Larval Central Nervous System    320.925 Larval Midgut    228.4 Larval Hindgut    348.2 Larval Malpighian Tubules    459.8 Larval Fat Body    333.7 Larval Salivary Gland    247.2 Larval Trachea    520.475 Larval Carcass    392.025 Adult Head    387.1 Adult Eye    627.575 Adult Brain    305.1 Adult Thoracic-Abdominal Ganglion    315.3 Adult Crop    457.6 Adult Midgut    199.7 Adult Hindgut    368.4 Adult Malpighian Tubules    425.8 Adult Fat Body    232.7 Adult Salivary Gland    406.3 Adult Heart    368.575 Adult VirginFemale Spermatheca    263.9 Adult InseminatedFemale Spermatheca    252.2 Adult Ovary    168.6 Adult Testis    355.9 Adult Male Accessory Gland    412.5 Adult Carcass    427.1 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    320.925 Larval Midgut    228.4 Larval Hindgut    348.2 Larval Malpighian Tubules    459.8 Larval Fat Body    333.7 Larval Salivary Gland    247.2 Larval Trachea  520.475 Larval Carcass    392.025 Adult Head    387.1 Adult Eye  (627.575) Adult Brain    305.1 Adult Thoracic-Abdominal Ganglion    315.3 Adult Crop    457.6 Adult Midgut    199.7 Adult Hindgut    368.4 Adult Malpighian Tubules    425.8 Adult Fat Body    232.7 Adult Salivary Gland    406.3 Adult Heart    368.575 Adult VirginFemale Spermatheca    263.9 Adult InseminatedFemale Spermatheca    252.2 Adult Ovary    168.6 Adult Testis    355.9 Adult Male Accessory Gland    412.5 Adult Carcass    427.1 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    320.925 Larval Midgut    228.4 Larval Hindgut    348.2 Larval Malpighian Tubules    459.8 Larval Fat Body    333.7 Larval Salivary Gland    247.2 Larval Trachea    520.475 Larval Carcass    392.025 Adult Head    387.1 Adult Eye    627.575 Adult Brain    305.1 Adult Thoracic-Abdominal Ganglion    315.3 Adult Crop    457.6 Adult Midgut    199.7 Adult Hindgut    368.4 Adult Malpighian Tubules    425.8 Adult Fat Body    232.7 Adult Salivary Gland    406.3 Adult Heart    368.575 Adult VirginFemale Spermatheca    263.9 Adult InseminatedFemale Spermatheca    252.2 Adult Ovary    168.6 Adult Testis    355.9 Adult Male Accessory Gland    412.5 Adult Carcass    427.1 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    320.925 Larval Midgut    228.4 Larval Hindgut    348.2 Larval Malpighian Tubules    459.8 Larval Fat Body    333.7 Larval Salivary Gland    247.2 Larval Trachea    520.475 Larval Carcass    392.025 Adult Head    387.1 Adult Eye    627.575 Adult Brain    305.1 Adult Thoracic-Abdominal Ganglion    315.3 Adult Crop    457.6 Adult Midgut    199.7 Adult Hindgut    368.4 Adult Malpighian Tubules    425.8 Adult Fat Body    232.7 Adult Salivary Gland    406.3 Adult Heart    368.575 Adult VirginFemale Spermatheca    263.9 Adult InseminatedFemale Spermatheca    252.2 Adult Ovary    168.6 Adult Testis    355.9 Adult Male Accessory Gland    412.5 Adult Carcass    427.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0086768 expression level Tissue   Expression Level Larval Central Nervous System    320.925 Larval Midgut    228.4 Larval Hindgut    348.2 Larval Malpighian Tubules    459.8 Larval Fat Body    333.7 Larval Salivary Gland    247.2 Larval Trachea    520.475 Larval Carcass    392.025 Adult Head    387.1 Adult Eye    627.575 Adult Brain    305.1 Adult Thoracic-Abdominal Ganglion    315.3 Adult Crop    457.6 Adult Midgut    199.7 Adult Hindgut    368.4 Adult Malpighian Tubules    425.8 Adult Fat Body    232.7 Adult Salivary Gland    406.3 Adult Heart    368.575 Adult VirginFemale Spermatheca    263.9 Adult InseminatedFemale Spermatheca    252.2 Adult Ovary    168.6 Adult Testis    355.9 Adult Male Accessory Gland    412.5 Adult Carcass    427.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    320.925 Larval Midgut    228.4 Larval Hindgut    348.2 Larval Malpighian Tubules    459.8 Larval Fat Body    333.7 Larval Salivary Gland    247.2 Larval Trachea  520.475 Larval Carcass    392.025 Adult Head    387.1 Adult Eye  627.575 Adult Brain    305.1 Adult Thoracic-Abdominal Ganglion    315.3 Adult Crop    457.6 Adult Midgut    199.7 Adult Hindgut    368.4 Adult Malpighian Tubules    425.8 Adult Fat Body    232.7 Adult Salivary Gland    406.3 Adult Heart    368.575 Adult VirginFemale Spermatheca    263.9 Adult InseminatedFemale Spermatheca    252.2 Adult Ovary    168.6 Adult Testis    355.9 Adult Male Accessory Gland    412.5 Adult Carcass    427.1 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    320.925 Larval Midgut    228.4 Larval Hindgut    348.2 Larval Malpighian Tubules    459.8 Larval Fat Body    333.7 Larval Salivary Gland    247.2 Larval Trachea    520.475 Larval Carcass    392.025 Adult Head    387.1 Adult Eye    627.575 Adult Brain    305.1 Adult Thoracic-Abdominal Ganglion    315.3 Adult Crop    457.6 Adult Midgut    199.7 Adult Hindgut    368.4 Adult Malpighian Tubules    425.8 Adult Fat Body    232.7 Adult Salivary Gland    406.3 Adult Heart    368.575 Adult VirginFemale Spermatheca    263.9 Adult InseminatedFemale Spermatheca    252.2 Adult Ovary    168.6 Adult Testis    355.9 Adult Male Accessory Gland    412.5 Adult Carcass    427.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    320.925 Larval Midgut    228.4 Larval Hindgut    348.2 Larval Malpighian Tubules    459.8 Larval Fat Body    333.7 Larval Salivary Gland    247.2 Larval Trachea    520.475 Larval Carcass    392.025 Adult Head    387.1 Adult Eye    627.575 Adult Brain    305.1 Adult Thoracic-Abdominal Ganglion    315.3 Adult Crop    457.6 Adult Midgut    199.7 Adult Hindgut    368.4 Adult Malpighian Tubules    425.8 Adult Fat Body    232.7 Adult Salivary Gland    406.3 Adult Heart    368.575 Adult VirginFemale Spermatheca    263.9 Adult InseminatedFemale Spermatheca    252.2 Adult Ovary    168.6 Adult Testis    355.9 Adult Male Accessory Gland    412.5 Adult Carcass    427.1 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    387.1   NA  Eye    627.575   NA  Brain    305.1   320.925  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    315.3   NA  Crop    457.6   228.4  Midgut    199.7   348.2  Hindgut    368.4   459.8  Malpighian Tubules    425.8   333.7  Fat Body    232.7   247.2  Salivary Gland    406.3   NA  Heart    368.575   520.475  Trachea    NA   NA  VirginFemale Spermatheca    263.9   NA  InseminatedFemale Spermatheca    252.2   NA  Ovary    168.6   NA  Testis    355.9   NA  Male Accessory Gland    412.5   392.025  Carcass    427.1 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_21
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0086768
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable Pcmtf00979 viable, with Scer\GAL4pnr-MD237 PcmtGD8637 Sterility Allele fertile Pcmtf00979 fertile, with Scer\GAL4hs.PB PcmtScer\UAS.cCa Other Phenotypes Allele locomotor behavior defective, with Scer\GAL4Act5C.PHb PcmtScer\UAS.cCa locomotor behavior defective, with Scer\GAL4hs.PB PcmtScer\UAS.cCa long lived, with Scer\GAL4Act5C.PHb PcmtScer\UAS.cCa long lived, with Scer\GAL4hs.PB PcmtScer\UAS.cCa Phenotype manifest in Allele
Classical Alleles ( 3 )
For All Classical Alleles Show
Allele of Pcmt Class Mutagen Stocks Known lesion Pcmtf00979 piggyBac transposase 2 -- PcmtG4610 P-element activity 1 -- PcmtLT P-element activity 1 Yes
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Pcmt Class Mutagen Stocks Known lesion PcmtScer\UAS.cCa in vitro construct - regulatory fusion 3 Yes PcmtdsRNA.IR.Scer\UAS in vitro construct - regulatory fusion in vitro construct - RNAi 2 Yes PcmtGD8637 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes PcmtHMS00768 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes
Aneuploid Aberrations
Partially disrupted in	Df(3R)LT (O'Connor and Tanini, 2009.6.24)
Disrupted in	Df(3R)Exel6144 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD8637} NA P{TRiP.HMS00768} NA P{UAS-Pcmt.C} NA P{UAS-Pcmt.IR} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{3'EPgy2}LT NA PBac{WH}Pcmtf00979 NA insertion of mobile activating element P{EP}PcmtG4610 NA
Gene Ontology: Function, Process & Cellular Component ( 4 unique terms )
Terms Based on Experimental Evidence ( 1 term )
Molecular Function
CV term Evidence References
protein-L-isoaspartate (D-aspartate) O-methyltransferase activity inferred from direct assay (Bennett et al., 2003)
Biological Process ( 0 terms)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
protein-L-isoaspartate (D-aspartate) O-methyltransferase activity inferred from sequence or structural similarity with mouse Pcmt1 (FlyBase, 1992-) non-traceable author statement (O'Connor, 1995.12.21, O'Connor, 1995.10.2, Galus et al., 1995)
Biological Process
CV term Evidence References
protein modification process inferred from electronic annotation with InterPro:IPR000682 (FlyBase Curators et al., 2004-) non-traceable author statement (Swiss-Prot Project Members, 1997.2.1) protein repair non-traceable author statement (O'Connor, 1995.10.2)
Cellular Component
CV term Evidence References
cytoplasm non-traceable author statement (Swiss-Prot Project Members, 1997.2.1)
Sequence Ontology: Class of Gene
	gene   nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Pcmt allele Gene References
External Data
Linkouts	DPiM - DPiM, Drosophila Protein interaction Map • FBgn0086768 DroID - A comprehensive database of gene and protein interactions. • FBgn0086768 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 40668
Orthologs
Genome-wide drosophilid orthologs
Curated drosophilid orthologs	Dpse\GA15271
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL001465-PA Aedes aegypti (mosquito) AAEL015520-PA Aedes aegypti (mosquito) AGAP000457-PA Anopheles gambiae (mosquito) XP_392316.2 Apis mellifera (honey bee) XP_001946333.1 Acyrthosiphon pisum (pea aphid) At3g48330.1 Arabidopsis thaliana (wall cress) 284774 Branchiostoma floridae (lancelet) BGIBMGA004956-PA Bombyx mori (silk worm) ENSBTAP00000013464 Bos taurus (bovine) CN15021 Caenorhabditis brenneri (small nematode) CBP08255 Caenorhabditis briggsae (nematode) CE08070 Caenorhabditis elegans (worm) ENSCAFP00000000571 Canis familiaris (dog) ENSCINP00000014934 Ciona intestinalis (sea squirt) JA11151 Caenorhabditis japonica (nematode) cn02244 Cryptococcus neoformans (yeast-like fungus) CPIJ016406 Culex pipens (mosquito) ENSCPOP00000010442 Cavia porcellus (guinea pig) 117339 Chlamidomonas reinhardtii (green alga) RP21615 Caenorhabditis remanei (nematode) 159419 Capitella spl (polychaete worm) DDB0214947 Dictyostelium discoideum (amoeba) 47356 Daphnia pulex (daphnia) ENSDARP00000087150 Danio rerio (zebra fish) ENSECAP00000004162 Equus caballus (horse) NP_417223.1 Escherichia coli K12 (bacteria) ENSGACP00000009603 Gymnopilus aculeatus (mushroom) ENSGALP00000007384 Gallus gallus (chicken) 155661 Hypsipyla robusta (cedar shoot borer) ENSP00000356354 Homo sapiens (human) ISCW019420-PA Ixodes scapularis (deer tick) 178722 Lottia gigantea (owl limpet) LmjF36.6550 Leishmania major (virus) 25262 Monocosta brevicollis (choanoflagellate) ENSMODP00000020973 Monodelphis domestica (gray opossum) ENSMMUP00000032338 Macaca mulatta (rhesus monkey) ENSMMUP00000033197 Macaca mulatta (rhesus monkey) ENSMUSP00000090386 Mus musculus (house mouse) 136839 Nematostella vectensis (starlet anemone) XP_001602813.1 Nasonia vitripennis (wasp) ENSOANP00000001700 Ornithorynchus anatinus (platypus) ENSORLP00000022053 Oryzias latipes (japanese killifish) LOC_Os04g40540.1 Oryza sativa (asian rice) 18817 Ostreococcus tauri (green alga) PF14_0309 Plasmodium falciparum (protozoal parasite) PHUM002859-PA Pediculus humanus (human body louse) 205982 Physcomitrella patens (moss) ENSPPYP00000019138 Pongo pygmaeus (orangutan) 73768 Phytophthora ramorum (sudden oak death pathogen) 113172 Phytophthora sojae (root rot pathogen) 423236 Populus trichocarpa (black cottonwood) ENSPTRP00000031911 Pan troglodytes (chimpanzee) PVX_084910 Plasmodium vivax (protozoal parasite) ENSRNOP00000050469 Rattus norvegicus (norway rat) 4887980 Sorghum bicolor (sorghum) Smp_058140.2 Schistosoma mansoni (trematode parasite) SPAC869.08 Schizosaccharomyces pombe (fission yeast) XP_001189051.1 Strongylocentrotus purpuratus (purple sea urchin) 49594 Trichoplax adhaerens (metazoa) XP_971086.1 Tribolium castaneum (red flour beetle) Tc00.1047053503779.40 Trypanosoma cruzi (parasitic trypanosome) Tc00.1047053506247.410 Trypanosoma cruzi (parasitic trypanosome) ENSTNIP00000020466 Tetraodon nigroviridis (green spotted pufferfish) 9911 Thalassiosira pseudonana (marine diatom) ENSTRUP00000003135 Takifugu rubripes (pufferfish Takifugu) 3694.m00175 Tetrahymena thermophila (ciliate protozoa) ENSXETP00000034884 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV18571 NV15844 NV14733 LH14641 ISCW019420 FBgn0241297 FBgn0215789 FBgn0211632 FBgn0191100 FBgn0165569 JGI_V11_47356 FBgn0075295 FBgn0159749 FBgn0147208 FBgn0129859 FBgn0103269 FBgn0096044 FBgn0093064 CPIJ016406 BGIBMGA004956 ACYPI004561 GB10967 GB10882 AGAP000457 AAEL015520 AAEL001465 PB23964 PHUM349660 GLEAN_07209
Stocks & Reagents
Stocks Listed in FlyBase ( 12 )
Bloomington	27396 P{UAS-Pcmt.IR}3-4 27397 P{UAS-Pcmt.IR}X-5 27393 w*; P{UAS-Pcmt.C}2-1 27394 P{UAS-Pcmt.C}X-2, w* 27395 w*; P{UAS-Pcmt.C}3-2 30088 w1118; P{EP}PcmtG4610 18398 w1118; PBac{WH}Pcmtf00979 32971 y1 sc* v1; P{TRiP.HMS00768}attP2 27595 y1 w*; Df(3R)LT, miaLT PcmtLT
Harvard	- PBac{WH}Pcmtf00979
VDRC	v19123 w1118; P{GD8637}v19123 v19121 w1118; P{GD8637}v19121/CyO
Genomic Clones ( 1 )
	BACR24O24 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 42 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH18536 RE73839 RE74472
Other clones	DPiM_RE74472
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH02868 GH05805 GH06539 GH08495 GH12647 GH14106 GH22349 GH23543 GH25163 GM16369 GM29761 RE49529 RE54335 RE56086 RE69517 RH13626 RH14905 SD15082
Other clones	40359 43676 106282 117468 163406 164870 273177 CK01.33A.D6 Dmel_dig_005103_0677_1842 EK153762 EK162642 EK231712 EK265309 EK267940 EK269518 IP03246 IP07604 RC0-DA0008-130700-012-E07 RC0-DA0008-130700-013-D02 RC0-DA0008-130700-013-G07
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0086768 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 40668 5657763
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Pcmt CG2152 (Gong, 1999.11)
Source for database merge of	Source for merge of: Pcmt Pimt (Bennett et al., 2003)
Additional comments	FlyBase curator comment: As pointed out by Terence Murphy of NCBI, the amino acid sequence given in Figure 1 for Pimt is identical to that of Pcmt. Therefore, this publication is the source of the merge for the two genes. (Bennett et al., 2003)
Other Comments
	Nonsense-mediated mRNA decay (NMD) down-regulates a distinct splice isoform(s) of this gene. (Hansen et al., 2009) Pcmt has been cloned and sequenced. (O'Connor et al., 1997)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_080508 RefSeq (Proteins) • NP_536756 DDBJ / EMBL / GenBank • AAA80540 AAA86272 AAF52012 AAF52012.1 AAL48101 AAL68334 AAN13241.1 AY070630 AY075527 BI579760 BI580301 U37432 U43737 Entrez Gene - A searchable database of RefSeq genes. • 40668 UniProtKB/Swiss-Prot • Q27869
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Protein-L-isoaspartate(D-aspartate) O-methyltransferase (IPR000682)
Linkouts
	DPiM - DPiM, Drosophila Protein interaction Map • FBgn0086768 DroID - A comprehensive database of gene and protein interactions. • FBgn0086768 DRSC - Results from RNAi screens. • FBgn0086768 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0086768 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0086768 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 40668 5657763 InParanoid A subset of ortholog calls from InParanoid. • AAEL001465-PA Aedes aegypti (mosquito) AAEL015520-PA Aedes aegypti (mosquito) AGAP000457-PA Anopheles gambiae (mosquito) XP_392316.2 Apis mellifera (honey bee) XP_001946333.1 Acyrthosiphon pisum (pea aphid) At3g48330.1 Arabidopsis thaliana (wall cress) 284774 Branchiostoma floridae (lancelet) BGIBMGA004956-PA Bombyx mori (silk worm) ENSBTAP00000013464 Bos taurus (bovine) CN15021 Caenorhabditis brenneri (small nematode) CBP08255 Caenorhabditis briggsae (nematode) CE08070 Caenorhabditis elegans (worm) ENSCAFP00000000571 Canis familiaris (dog) ENSCINP00000014934 Ciona intestinalis (sea squirt) JA11151 Caenorhabditis japonica (nematode) cn02244 Cryptococcus neoformans (yeast-like fungus) CPIJ016406 Culex pipens (mosquito) ENSCPOP00000010442 Cavia porcellus (guinea pig) 117339 Chlamidomonas reinhardtii (green alga) RP21615 Caenorhabditis remanei (nematode) 159419 Capitella spl (polychaete worm) DDB0214947 Dictyostelium discoideum (amoeba) 47356 Daphnia pulex (daphnia) ENSDARP00000087150 Danio rerio (zebra fish) ENSECAP00000004162 Equus caballus (horse) NP_417223.1 Escherichia coli K12 (bacteria) ENSGACP00000009603 Gymnopilus aculeatus (mushroom) ENSGALP00000007384 Gallus gallus (chicken) 155661 Hypsipyla robusta (cedar shoot borer) ENSP00000356354 Homo sapiens (human) ISCW019420-PA Ixodes scapularis (deer tick) 178722 Lottia gigantea (owl limpet) LmjF36.6550 Leishmania major (virus) 25262 Monocosta brevicollis (choanoflagellate) ENSMODP00000020973 Monodelphis domestica (gray opossum) ENSMMUP00000032338 Macaca mulatta (rhesus monkey) ENSMMUP00000033197 Macaca mulatta (rhesus monkey) ENSMUSP00000090386 Mus musculus (house mouse) 136839 Nematostella vectensis (starlet anemone) XP_001602813.1 Nasonia vitripennis (wasp) ENSOANP00000001700 Ornithorynchus anatinus (platypus) ENSORLP00000022053 Oryzias latipes (japanese killifish) LOC_Os04g40540.1 Oryza sativa (asian rice) 18817 Ostreococcus tauri (green alga) PF14_0309 Plasmodium falciparum (protozoal parasite) PHUM002859-PA Pediculus humanus (human body louse) 205982 Physcomitrella patens (moss) ENSPPYP00000019138 Pongo pygmaeus (orangutan) 73768 Phytophthora ramorum (sudden oak death pathogen) 113172 Phytophthora sojae (root rot pathogen) 423236 Populus trichocarpa (black cottonwood) ENSPTRP00000031911 Pan troglodytes (chimpanzee) PVX_084910 Plasmodium vivax (protozoal parasite) ENSRNOP00000050469 Rattus norvegicus (norway rat) 4887980 Sorghum bicolor (sorghum) Smp_058140.2 Schistosoma mansoni (trematode parasite) SPAC869.08 Schizosaccharomyces pombe (fission yeast) XP_001189051.1 Strongylocentrotus purpuratus (purple sea urchin) 49594 Trichoplax adhaerens (metazoa) XP_971086.1 Tribolium castaneum (red flour beetle) Tc00.1047053503779.40 Trypanosoma cruzi (parasitic trypanosome) Tc00.1047053506247.410 Trypanosoma cruzi (parasitic trypanosome) ENSTNIP00000020466 Tetraodon nigroviridis (green spotted pufferfish) 9911 Thalassiosira pseudonana (marine diatom) ENSTRUP00000003135 Takifugu rubripes (pufferfish Takifugu) 3694.m00175 Tetrahymena thermophila (ciliate protozoa) ENSXETP00000034884 Xenopus tropicalis (frog) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 40668 modMine - Data generated by the modENCODE project. • FBgn0086768 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV18571 NV15844 NV14733 LH14641 ISCW019420 FBgn0241297 FBgn0215789 FBgn0211632 FBgn0191100 FBgn0165569 JGI_V11_47356 FBgn0075295 FBgn0159749 FBgn0147208 FBgn0129859 FBgn0103269 FBgn0096044 FBgn0093064 CPIJ016406 BGIBMGA004956 ACYPI004561 GB10967 GB10882 AGAP000457 AAEL015520 AAEL001465 PB23964 PHUM349660 GLEAN_07209
Synonyms & Secondary IDs ( 9 )
Reported As
Symbol Synonym	CG2152 (Ayres et al., 2008, Hansen et al., 2009, Ni et al., 2010.12.1) dPIMT (Bennett et al., 2003) pcmt (Ayres et al., 2008) Pcmt (O'Connor, 2009.5.11, O'Connor and Tanini, 2009.6.24, Hansen et al., 2009) PIMT (David et al., 1999, Galus et al., 1995) Pimt
Name Synonym	protein D-aspartyl, L-isoaspartyl O-methyltransferase (O'Connor, 1995.10.2) Protein isoaspartyl methyltransferase (Galus et al., 1995) Protein-L-isoaspartate (D-aspartate) O-methyltransferase
Secondary FlyBase IDs
	FBgn0015276 FBgn0015280 FBgn0022769
References ( 32 )
Generate a list of	All references relating to this gene ( 32 )
List References by type	Research paper  ( 7 ) Supplementary material  ( 2 ) Personal communication to FlyBase  ( 9 ) Abstract  ( 5 ) FlyBase analysis  ( 1 ) DNA/RNA sequence record  ( 2 ) Curated genome annotation  ( 2 ) Protein sequence record  ( 1 ) Computer file  ( 1 ) Automatic genome annotation  ( 1 )
Recent research papers (0)
	All research papers listed in FlyBase were published before 2010 Show research papers ...