PACAP38, Drm-amn, cheapdate, PACAP
Gene model reviewed during 5.46
Gene model uncertain: The transcript structure and CDS of this gene are not well supported; it is predicted to encode a small neuropeptide (FBrf0132261). Although the CDS as annotated is not conserved, the region corresponding to the predicted post-translationally processed neuropeptide is conserved in the closer Sophophora species.
There is only one protein coding transcript and one polypeptide associated with this gene
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\amn using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\amn in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
The expression pattern of amn, together with targetted mutant phenotypic analysis suggests a modulatory role for amn in memory formation. amn neuropeptide release from the dorsal paired medial cells onto the mushroom bodies is critical for normal olfactory memory.
The larval CNS and neuromuscular junctions have a PACAP-like immunoreactivity. Antibodies raised against mammalian PACAP38 identify a polypeptide of a similar size. Pacap38 may induce two temporally separated muscle responses: an early depolarisation and a late modulation of voltage-activated potassium currents. Muscle response is evoked by a high frequency stimulation of larval motor axons.
Homozygous or hemizygous mutant flies can be conditioned to avoid odors associated with electric shocks, but effects of conditioning decay with a half life of 15 min compared to 60 min for normal. Memory decay biphasic; rapid for first hour and slow thereafter.
Mutations in amn significantly reduce the females song memory after prestimulation with courtship hums. This suggests a simple sensitization process may be involved with the female pulse song memory (FBrf0041559).
Memory retention curves and retrograde amnesia experiments suggest the amn mutations interferes with early memory formation.
The effects on courtship behavior or pre-exposure to fertilized females decay more rapidly in amn than in normal males.
Females defective in ability to be primed by courtship song.
Substitution of reward (1.0 M sucrose) for punishment (electric shock) lengthens memory span from one hour to six hours.