Gene Dmel\stmA

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General Information

Symbol

Dmel\stmA

Species

D. melanogaster

Name

stambha A

Annotation symbol

CG8739

Feature type

protein_coding_gene

FlyBase ID

FBgn0086784

Gene Model Status

Current

Stock availability

18 publicly available

Also Known As

cmp44E, rbo

Genomic Location

Chromosome (arm)

Recombination map

2-56.8

Cytogenetic map

44E3-44E3

Sequence location

2R:4,617,007..4,621,809 [-]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

The gene stambha A is referred to in FlyBase by the symbol Dmel\stmA (CG8739, FBgn0086784). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: synaptic transmission; phototransduction; regulation of endocytosis; synaptic vesicle endocytosis; cytokinesis; synaptic vesicle exocytosis. 53 alleles are reported. The phenotypes of these alleles are annotated with: organ system subdivision; organ system; multicellular structure; adult segment; synapse; portion of tissue; extended germ band embryo; cytoplasmic part; sensory cluster; external compound sense organ; late extended germ band embryo. It has 4 annotated transcripts and 4 annotated polypeptides. Protein features are: Armadillo-like helical; Armadillo-type fold. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 00-06 and 12-24 hour embryonic stages, during early larval stages, during late pupal stages, in stages of adults of both sexes. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult central nervous system. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, adult Malpighian tubules, adult fat body, larval/adult salivary gland, adult ovary, adult male reproductive system, adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1641233_a_at completely aligns to an exonic region common to each of the 3 FlyBase-annotated transcript isoforms of stmA. Gene sequence location is 2R:4617007..4621809.

User Contributed Data

Beta version, feedback welcome

Phenotypic Description from the Red Book (Lindsley & Zimm 1992)

Gene/Allele symbols may differ from current usage

stm-A

Two-hour heat shocks applied throughout development show 0-8 h and 56-140 h to be highly sensitive, with no survival; 8-56 h and after 140 h show survival with normal phenotype. Early temperature shock leads to blastoderm arrest with no gastrulation. Embryos produced by homozygous stm-A mothers are exceedingly sensitive to a two-hour exposure to 36; leads to complete lethality from 0-3 h irrespective of paternal contribution.

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2013_03

FB2013_02

All updates

Click here to see a list of all updates to this record from FB2010_08 and on.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

44E3-44E3

Limits computationally determined from genome sequence between P{PZ}l(2)44DEa⁰⁵⁸⁴⁷ and P{lacW}Rya-r44F^k04913

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

44D1-44D2

FBrf0065355 concluded that stmA mapped to 42A16-42B2, however deficiency mapping in FBrf0144960 suggests that stmA maps to 44D1-44D2.

(Kumar et al., 2001)

44E3-44E4

(Faulkner, 1998.10.15)

Experimentally Determined Recombination Data

Location

2-56.8

(Chandrashekaran and Sarla, 1993, Chandrashekaran, 1993)

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\stmA for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.49

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

stmA-RA

FBtr0088716

NM_165625

2738

794

stmA-RB

FBtr0088715

NM_165624

3116

820

stmA-RC

FBtr0301639

NM_001014513

3158

834

stmA-RD

FBtr0339341

2780

808

Additional Transcript Data & Comments

Reported size (kB)

3.4, 2.9 (northern blot)

(Faulkner et al., 1998)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

stmA-PA

FBpp0087795

89.3

794

6.85

NP_724711

AAF59046

stmA-PB

FBpp0087794

92.4

820

6.62

NP_724710

AAF59047

stmA-PC

FBpp0100147

94.0

834

6.76

NP_001014513

AAX52723

stmA-PD

FBpp0308439

90.9

808

7.02

Additional Polypeptide Data & Comments

Reported size (kDa)

794 (aa); 91 (kD predicted)

(Faulkner et al., 1998)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Armadillo-type fold (IPR016024)

Armadillo-like helical (IPR011989)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q8IGJ0

UniProtKB/TrEMBL

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism | ubiquitous

(Faulkner et al., 1998)

embryonic brain

(Faulkner et al., 1998)

presumptive embryonic/larval central nervous system

(Faulkner et al., 1998)

embryonic stage 1 -- 3

organism

(Fisher et al., 2012)

Comment:maternally deposited

embryonic stage 4 -- 6

organism

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic/larval muscle system

(Fisher et al., 2012)

adult stage & oogenesis

germarium

(Faulkner et al., 1998)

follicle cell

(Faulkner et al., 1998)

adult stage oogenesis stage S1 -- S5

nurse cell

(Faulkner et al., 1998)

adult stage oogenesis stage S9 -- S14

(Faulkner et al., 1998)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage & adult stage

(Faulkner et al., 1998)

embryonic stage -- adult stage

(Faulkner et al., 1998)

Additional Descriptive Data

The 2.9kb stmA transcript is detected throughout development and in adults on northern blots.

(Faulkner et al., 1998)

The 3.4kb stmA transcript is detected in 12-23hr embryos and in adults. It is present at lower abundance than the 2.9kb transcript.

(Faulkner et al., 1998)

stmA transcripts are ubiquitously distributed in the first half of embryogenesis. They later become more concentrated in the CNS and brain. stmA transcripts are detected in the germline throughout the germarial stages. They are expressed in nurse cells until stage 5 and then are not detected again until stage 9, at which time they are present at high levels. Lower levels are also observed in follicle cells.

(Faulkner et al., 1998)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Wasbrough et al., 2010)

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

synapse

inferred from direct assay

(Vijayakrishnan et al., 2009)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult central nervous system. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, adult Malpighian tubules, adult fat body, larval/adult salivary gland, adult ovary, adult male reproductive system, adult carcass.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		150.025
Larval Midgut		100.2
Larval Hindgut		164.7
Larval Malpighian Tubules		60.2
Larval Fat Body		75.5
Larval Salivary Gland		114.5
Larval Trachea		90.075
Larval Carcass		92.3
Adult Head		333.5
Adult Eye		305.725
Adult Brain		1273.1
Adult Thoracic-Abdominal Ganglion		1072.4
Adult Crop		193.8
Adult Midgut		141.2
Adult Hindgut		357.1
Adult Malpighian Tubules		147.9
Adult Fat Body		135.9
Adult Salivary Gland		170.2
Adult Heart		98.45
Adult VirginFemale Spermatheca		78.9
Adult InseminatedFemale Spermatheca		82.7
Adult Ovary		146.6
Adult Testis		113.9
Adult Male Accessory Gland		137.7
Adult Carcass		152.8
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		150.025
Larval Midgut		100.2
Larval Hindgut		164.7
Larval Malpighian Tubules		60.2
Larval Fat Body		75.5
Larval Salivary Gland		114.5
Larval Trachea		90.075
Larval Carcass		92.3
Adult Head		333.5
Adult Eye		305.725
Adult Brain		(1273.1)
Adult Thoracic-Abdominal Ganglion		(1072.4)
Adult Crop		193.8
Adult Midgut		141.2
Adult Hindgut		357.1
Adult Malpighian Tubules		147.9
Adult Fat Body		135.9
Adult Salivary Gland		170.2
Adult Heart		98.45
Adult VirginFemale Spermatheca		78.9
Adult InseminatedFemale Spermatheca		82.7
Adult Ovary		146.6
Adult Testis		113.9
Adult Male Accessory Gland		137.7
Adult Carcass		152.8
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		150.025
Larval Midgut		100.2
Larval Hindgut		164.7
Larval Malpighian Tubules		60.2
Larval Fat Body		75.5
Larval Salivary Gland		114.5
Larval Trachea		90.075
Larval Carcass		92.3
Adult Head		333.5
Adult Eye		305.725
Adult Brain		(1273.1)
Adult Thoracic-Abdominal Ganglion		1072.4
Adult Crop		193.8
Adult Midgut		141.2
Adult Hindgut		357.1
Adult Malpighian Tubules		147.9
Adult Fat Body		135.9
Adult Salivary Gland		170.2
Adult Heart		98.45
Adult VirginFemale Spermatheca		78.9
Adult InseminatedFemale Spermatheca		82.7
Adult Ovary		146.6
Adult Testis		113.9
Adult Male Accessory Gland		137.7
Adult Carcass		152.8
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		150.025
Larval Midgut		100.2
Larval Hindgut		164.7
Larval Malpighian Tubules		60.2
Larval Fat Body		75.5
Larval Salivary Gland		114.5
Larval Trachea		90.075
Larval Carcass		92.3
Adult Head		333.5
Adult Eye		305.725
Adult Brain		1273.1
Adult Thoracic-Abdominal Ganglion		1072.4
Adult Crop		193.8
Adult Midgut		141.2
Adult Hindgut		357.1
Adult Malpighian Tubules		147.9
Adult Fat Body		135.9
Adult Salivary Gland		170.2
Adult Heart		98.45
Adult VirginFemale Spermatheca		78.9
Adult InseminatedFemale Spermatheca		82.7
Adult Ovary		146.6
Adult Testis		113.9
Adult Male Accessory Gland		137.7
Adult Carcass		152.8
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		150.025
Larval Midgut		100.2
Larval Hindgut		164.7
Larval Malpighian Tubules		60.2
Larval Fat Body		75.5
Larval Salivary Gland		114.5
Larval Trachea		90.075
Larval Carcass		92.3
Adult Head		333.5
Adult Eye		305.725
Adult Brain		1273.1
Adult Thoracic-Abdominal Ganglion		1072.4
Adult Crop		193.8
Adult Midgut		141.2
Adult Hindgut		357.1
Adult Malpighian Tubules		147.9
Adult Fat Body		135.9
Adult Salivary Gland		170.2
Adult Heart		98.45
Adult VirginFemale Spermatheca		78.9
Adult InseminatedFemale Spermatheca		82.7
Adult Ovary		146.6
Adult Testis		113.9
Adult Male Accessory Gland		137.7
Adult Carcass		152.8
Expression Level Scale		None Low Moderate High Very high

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		150.025
Larval Midgut		100.2
Larval Hindgut		164.7
Larval Malpighian Tubules		60.2
Larval Fat Body		75.5
Larval Salivary Gland		114.5
Larval Trachea		90.075
Larval Carcass		92.3
Adult Head		333.5
Adult Eye		305.725
Adult Brain		(1273.1)
Adult Thoracic-Abdominal Ganglion		(1072.4)
Adult Crop		193.8
Adult Midgut		141.2
Adult Hindgut		357.1
Adult Malpighian Tubules		147.9
Adult Fat Body		135.9
Adult Salivary Gland		170.2
Adult Heart		98.45
Adult VirginFemale Spermatheca		78.9
Adult InseminatedFemale Spermatheca		82.7
Adult Ovary		146.6
Adult Testis		113.9
Adult Male Accessory Gland		137.7
Adult Carcass		152.8
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		150.025
Larval Midgut		100.2
Larval Hindgut		164.7
Larval Malpighian Tubules		60.2
Larval Fat Body		75.5
Larval Salivary Gland		114.5
Larval Trachea		90.075
Larval Carcass		92.3
Adult Head		333.5
Adult Eye		305.725
Adult Brain		1273.1
Adult Thoracic-Abdominal Ganglion		1072.4
Adult Crop		193.8
Adult Midgut		141.2
Adult Hindgut		357.1
Adult Malpighian Tubules		147.9
Adult Fat Body		135.9
Adult Salivary Gland		170.2
Adult Heart		98.45
Adult VirginFemale Spermatheca		78.9
Adult InseminatedFemale Spermatheca		82.7
Adult Ovary		146.6
Adult Testis		113.9
Adult Male Accessory Gland		137.7
Adult Carcass		152.8
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		150.025
Larval Midgut		100.2
Larval Hindgut		164.7
Larval Malpighian Tubules		60.2
Larval Fat Body		75.5
Larval Salivary Gland		114.5
Larval Trachea		90.075
Larval Carcass		92.3
Adult Head		333.5
Adult Eye		305.725
Adult Brain		1273.1
Adult Thoracic-Abdominal Ganglion		1072.4
Adult Crop		193.8
Adult Midgut		141.2
Adult Hindgut		357.1
Adult Malpighian Tubules		147.9
Adult Fat Body		135.9
Adult Salivary Gland		170.2
Adult Heart		98.45
Adult VirginFemale Spermatheca		78.9
Adult InseminatedFemale Spermatheca		82.7
Adult Ovary		146.6
Adult Testis		113.9
Adult Male Accessory Gland		137.7
Adult Carcass		152.8
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	333.5
NA	Eye	305.725
NA	Brain	1273.1
150.025	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	1072.4
NA	Crop	193.8
100.2	Midgut	141.2
164.7	Hindgut	357.1
60.2	Malpighian Tubules	147.9
75.5	Fat Body	135.9
114.5	Salivary Gland	170.2
NA	Heart	98.45
90.075	Trachea	NA
NA	VirginFemale Spermatheca	78.9
NA	InseminatedFemale Spermatheca	82.7
NA	Ovary	146.6
NA	Testis	113.9
NA	Male Accessory Gland	137.7
92.3	Carcass	152.8

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		15
central nervous system, larvae L3		14
central nervous system, pupae P8		16
head, virgin 1-day female		67
head, virgin 4-day female		50
head, virgin 20-day female		41
head, mated 1-day female		50
head, mated 4-day female		41
head, mated 20-day female		35
head, mated 1-day male		103
head, mated 4-day male		84
head, mated 20-day male		75
salivary gland, larvae L3 wandering		4
salivary gland, white prepupae		13
digestive system, larvae L3 wandering		12
digestive system, 1-day adult		16
digestive system, 4-day adult		13
digestive system, 20-day adult		15
fat body, larvae L3 wandering		6
fat body, white prepupae		11
fat body, pupae P8		18
carcass, larvae L3 wandering		14
carcass, 1-day adult		15
carcass, 4-day adult		15
carcass, 20-day adult		17
ovary, virgin 4-day female		29
ovary, mated 4-day female		22
testis, mated 4-day male		18
accessory gland, mated 4-day male		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		15
central nervous system, larvae L3		14
central nervous system, pupae P8		16
head, virgin 1-day female		(67)
head, virgin 4-day female		(50)
head, virgin 20-day female		(41)
head, mated 1-day female		(50)
head, mated 4-day female		(41)
head, mated 20-day female		(35)
head, mated 1-day male		(103)
head, mated 4-day male		(84)
head, mated 20-day male		(75)
salivary gland, larvae L3 wandering		4
salivary gland, white prepupae		13
digestive system, larvae L3 wandering		12
digestive system, 1-day adult		16
digestive system, 4-day adult		13
digestive system, 20-day adult		15
fat body, larvae L3 wandering		6
fat body, white prepupae		11
fat body, pupae P8		18
carcass, larvae L3 wandering		14
carcass, 1-day adult		15
carcass, 4-day adult		15
carcass, 20-day adult		17
ovary, virgin 4-day female		(29)
ovary, mated 4-day female		22
testis, mated 4-day male		18
accessory gland, mated 4-day male		9
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		15
central nervous system, larvae L3		14
central nervous system, pupae P8		16
head, virgin 1-day female		67
head, virgin 4-day female		50
head, virgin 20-day female		41
head, mated 1-day female		50
head, mated 4-day female		41
head, mated 20-day female		35
head, mated 1-day male		103
head, mated 4-day male		84
head, mated 20-day male		75
salivary gland, larvae L3 wandering		4
salivary gland, white prepupae		13
digestive system, larvae L3 wandering		12
digestive system, 1-day adult		16
digestive system, 4-day adult		13
digestive system, 20-day adult		15
fat body, larvae L3 wandering		6
fat body, white prepupae		11
fat body, pupae P8		18
carcass, larvae L3 wandering		14
carcass, 1-day adult		15
carcass, 4-day adult		15
carcass, 20-day adult		17
ovary, virgin 4-day female		29
ovary, mated 4-day female		22
testis, mated 4-day male		18
accessory gland, mated 4-day male		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		15
central nervous system, larvae L3		14
central nervous system, pupae P8		16
head, virgin 1-day female		67
head, virgin 4-day female		50
head, virgin 20-day female		41
head, mated 1-day female		50
head, mated 4-day female		41
head, mated 20-day female		35
head, mated 1-day male		103
head, mated 4-day male		84
head, mated 20-day male		75
salivary gland, larvae L3 wandering		4
salivary gland, white prepupae		13
digestive system, larvae L3 wandering		12
digestive system, 1-day adult		16
digestive system, 4-day adult		13
digestive system, 20-day adult		15
fat body, larvae L3 wandering		6
fat body, white prepupae		11
fat body, pupae P8		18
carcass, larvae L3 wandering		14
carcass, 1-day adult		15
carcass, 4-day adult		15
carcass, 20-day adult		17
ovary, virgin 4-day female		29
ovary, mated 4-day female		22
testis, mated 4-day male		18
accessory gland, mated 4-day male		9
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		15
central nervous system, larvae L3		14
central nervous system, pupae P8		16
head, virgin 1-day female		67
head, virgin 4-day female		50
head, virgin 20-day female		41
head, mated 1-day female		50
head, mated 4-day female		41
head, mated 20-day female		35
head, mated 1-day male		103
head, mated 4-day male		84
head, mated 20-day male		75
salivary gland, larvae L3 wandering		4
salivary gland, white prepupae		13
digestive system, larvae L3 wandering		12
digestive system, 1-day adult		16
digestive system, 4-day adult		13
digestive system, 20-day adult		15
fat body, larvae L3 wandering		6
fat body, white prepupae		11
fat body, pupae P8		18
carcass, larvae L3 wandering		14
carcass, 1-day adult		15
carcass, 4-day adult		15
carcass, 20-day adult		17
ovary, virgin 4-day female		29
ovary, mated 4-day female		22
testis, mated 4-day male		18
accessory gland, mated 4-day male		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		15
central nervous system, larvae L3		14
central nervous system, pupae P8		16
head, virgin 1-day female		(67)
head, virgin 4-day female		(50)
head, virgin 20-day female		(41)
head, mated 1-day female		(50)
head, mated 4-day female		(41)
head, mated 20-day female		(35)
head, mated 1-day male		(103)
head, mated 4-day male		(84)
head, mated 20-day male		(75)
salivary gland, larvae L3 wandering		4
salivary gland, white prepupae		13
digestive system, larvae L3 wandering		12
digestive system, 1-day adult		16
digestive system, 4-day adult		13
digestive system, 20-day adult		15
fat body, larvae L3 wandering		6
fat body, white prepupae		11
fat body, pupae P8		18
carcass, larvae L3 wandering		14
carcass, 1-day adult		15
carcass, 4-day adult		15
carcass, 20-day adult		17
ovary, virgin 4-day female		29
ovary, mated 4-day female		22
testis, mated 4-day male		18
accessory gland, mated 4-day male		9
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		15
central nervous system, larvae L3		14
central nervous system, pupae P8		16
head, virgin 1-day female		67
head, virgin 4-day female		50
head, virgin 20-day female		41
head, mated 1-day female		50
head, mated 4-day female		41
head, mated 20-day female		35
head, mated 1-day male		103
head, mated 4-day male		84
head, mated 20-day male		75
salivary gland, larvae L3 wandering		4
salivary gland, white prepupae		13
digestive system, larvae L3 wandering		12
digestive system, 1-day adult		16
digestive system, 4-day adult		13
digestive system, 20-day adult		15
fat body, larvae L3 wandering		6
fat body, white prepupae		11
fat body, pupae P8		18
carcass, larvae L3 wandering		14
carcass, 1-day adult		15
carcass, 4-day adult		15
carcass, 20-day adult		17
ovary, virgin 4-day female		29
ovary, mated 4-day female		22
testis, mated 4-day male		18
accessory gland, mated 4-day male		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		15
central nervous system, larvae L3		14
central nervous system, pupae P8		16
head, virgin 1-day female		67
head, virgin 4-day female		50
head, virgin 20-day female		41
head, mated 1-day female		50
head, mated 4-day female		41
head, mated 20-day female		35
head, mated 1-day male		103
head, mated 4-day male		84
head, mated 20-day male		75
salivary gland, larvae L3 wandering		4
salivary gland, white prepupae		13
digestive system, larvae L3 wandering		12
digestive system, 1-day adult		16
digestive system, 4-day adult		13
digestive system, 20-day adult		15
fat body, larvae L3 wandering		6
fat body, white prepupae		11
fat body, pupae P8		18
carcass, larvae L3 wandering		14
carcass, 1-day adult		15
carcass, 4-day adult		15
carcass, 20-day adult		17
ovary, virgin 4-day female		29
ovary, mated 4-day female		22
testis, mated 4-day male		18
accessory gland, mated 4-day male		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 00-06 and 12-24 hour embryonic stages, during early larval stages, during late pupal stages, in stages of adults of both sexes.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		38
embryo 02-04hr		16
embryo 04-06hr		13
embryo 06-08hr		14
embryo 08-10hr		15
embryo 10-12hr		16
embryo 12-14hr		14
embryo 14-16hr		18
embryo 16-18hr		22
embryo 18-20hr		25
embryo 20-22hr		21
embryo 22-24hr		23
larva L1		22
larva L2		16
larva L3 12hr old		15
larva L3 puffstage 1-2		13
larva L3 puffstage 3-6		15
larva L3 puffstage 7-9		15
white prepupae new		15
white prepupae 12hr		19
white prepupae 24hr		16
pupae 2d postWPP		20
pupae 3d postWPP		32
pupae 4d postWPP		42
adult male 01day		41
adult male 05day		41
adult male 30day		37
adult female 01day		32
adult female 05day		29
adult female 30day		30
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(38)
embryo 02-04hr		16
embryo 04-06hr		13
embryo 06-08hr		14
embryo 08-10hr		15
embryo 10-12hr		16
embryo 12-14hr		14
embryo 14-16hr		18
embryo 16-18hr		22
embryo 18-20hr		25
embryo 20-22hr		21
embryo 22-24hr		23
larva L1		22
larva L2		16
larva L3 12hr old		15
larva L3 puffstage 1-2		13
larva L3 puffstage 3-6		15
larva L3 puffstage 7-9		15
white prepupae new		15
white prepupae 12hr		19
white prepupae 24hr		16
pupae 2d postWPP		20
pupae 3d postWPP		(32)
pupae 4d postWPP		(42)
adult male 01day		(41)
adult male 05day		(41)
adult male 30day		(37)
adult female 01day		(32)
adult female 05day		(29)
adult female 30day		(30)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		38
embryo 02-04hr		16
embryo 04-06hr		13
embryo 06-08hr		14
embryo 08-10hr		15
embryo 10-12hr		16
embryo 12-14hr		14
embryo 14-16hr		18
embryo 16-18hr		22
embryo 18-20hr		25
embryo 20-22hr		21
embryo 22-24hr		23
larva L1		22
larva L2		16
larva L3 12hr old		15
larva L3 puffstage 1-2		13
larva L3 puffstage 3-6		15
larva L3 puffstage 7-9		15
white prepupae new		15
white prepupae 12hr		19
white prepupae 24hr		16
pupae 2d postWPP		20
pupae 3d postWPP		32
pupae 4d postWPP		42
adult male 01day		41
adult male 05day		41
adult male 30day		37
adult female 01day		32
adult female 05day		29
adult female 30day		30
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		38
embryo 02-04hr		16
embryo 04-06hr		13
embryo 06-08hr		14
embryo 08-10hr		15
embryo 10-12hr		16
embryo 12-14hr		14
embryo 14-16hr		18
embryo 16-18hr		22
embryo 18-20hr		25
embryo 20-22hr		21
embryo 22-24hr		23
larva L1		22
larva L2		16
larva L3 12hr old		15
larva L3 puffstage 1-2		13
larva L3 puffstage 3-6		15
larva L3 puffstage 7-9		15
white prepupae new		15
white prepupae 12hr		19
white prepupae 24hr		16
pupae 2d postWPP		20
pupae 3d postWPP		32
pupae 4d postWPP		42
adult male 01day		41
adult male 05day		41
adult male 30day		37
adult female 01day		32
adult female 05day		29
adult female 30day		30
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		38
embryo 02-04hr		16
embryo 04-06hr		13
embryo 06-08hr		14
embryo 08-10hr		15
embryo 10-12hr		16
embryo 12-14hr		14
embryo 14-16hr		18
embryo 16-18hr		22
embryo 18-20hr		25
embryo 20-22hr		21
embryo 22-24hr		23
larva L1		22
larva L2		16
larva L3 12hr old		15
larva L3 puffstage 1-2		13
larva L3 puffstage 3-6		15
larva L3 puffstage 7-9		15
white prepupae new		15
white prepupae 12hr		19
white prepupae 24hr		16
pupae 2d postWPP		20
pupae 3d postWPP		32
pupae 4d postWPP		42
adult male 01day		41
adult male 05day		41
adult male 30day		37
adult female 01day		32
adult female 05day		29
adult female 30day		30
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(38)
embryo 02-04hr		16
embryo 04-06hr		13
embryo 06-08hr		14
embryo 08-10hr		15
embryo 10-12hr		16
embryo 12-14hr		14
embryo 14-16hr		18
embryo 16-18hr		22
embryo 18-20hr		25
embryo 20-22hr		21
embryo 22-24hr		23
larva L1		22
larva L2		16
larva L3 12hr old		15
larva L3 puffstage 1-2		13
larva L3 puffstage 3-6		15
larva L3 puffstage 7-9		15
white prepupae new		15
white prepupae 12hr		19
white prepupae 24hr		16
pupae 2d postWPP		20
pupae 3d postWPP		32
pupae 4d postWPP		(42)
adult male 01day		(41)
adult male 05day		(41)
adult male 30day		(37)
adult female 01day		32
adult female 05day		29
adult female 30day		30
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		38
embryo 02-04hr		16
embryo 04-06hr		13
embryo 06-08hr		14
embryo 08-10hr		15
embryo 10-12hr		16
embryo 12-14hr		14
embryo 14-16hr		18
embryo 16-18hr		22
embryo 18-20hr		25
embryo 20-22hr		21
embryo 22-24hr		23
larva L1		22
larva L2		16
larva L3 12hr old		15
larva L3 puffstage 1-2		13
larva L3 puffstage 3-6		15
larva L3 puffstage 7-9		15
white prepupae new		15
white prepupae 12hr		19
white prepupae 24hr		16
pupae 2d postWPP		20
pupae 3d postWPP		32
pupae 4d postWPP		42
adult male 01day		41
adult male 05day		41
adult male 30day		37
adult female 01day		32
adult female 05day		29
adult female 30day		30
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		38
embryo 02-04hr		16
embryo 04-06hr		13
embryo 06-08hr		14
embryo 08-10hr		15
embryo 10-12hr		16
embryo 12-14hr		14
embryo 14-16hr		18
embryo 16-18hr		22
embryo 18-20hr		25
embryo 20-22hr		21
embryo 22-24hr		23
larva L1		22
larva L2		16
larva L3 12hr old		15
larva L3 puffstage 1-2		13
larva L3 puffstage 3-6		15
larva L3 puffstage 7-9		15
white prepupae new		15
white prepupae 12hr		19
white prepupae 24hr		16
pupae 2d postWPP		20
pupae 3d postWPP		32
pupae 4d postWPP		42
adult male 01day		41
adult male 05day		41
adult male 30day		37
adult female 01day		32
adult female 05day		29
adult female 30day		30
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		16
Schneider line 2 Sg4		12
embryonic 1182-4H		16
embryonic GM2		22
embryonic Kc167		17
embryonic S1		20
embryonic S3		15
leg disc CME L1		16
wing disc CME-W2		13
wing disc ML-DmD8		11
wing disc ML-DmD9		16
wing disc ML-DmD16-c3		17
wing disc ML-DmD21		22
wing disc ML-DmD32		15
haltere disc ML-DmD17-c3		16
eye-antennal disc ML-DmD11		15
antennal disc ML-DmD20-c5		19
mixed discs ML-DmD4-c1		14
CNS ML-DmBG1-c1		16
CNS ML-DmBG2-c2		23
tumorous blood cells mbn2		15
ovary fGS/OSS		22
ovary OSC		14
ovary OSS		9
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		16
Schneider line 2 Sg4		12
embryonic 1182-4H		16
embryonic GM2		22
embryonic Kc167		17
embryonic S1		20
embryonic S3		15
leg disc CME L1		16
wing disc CME-W2		13
wing disc ML-DmD8		11
wing disc ML-DmD9		16
wing disc ML-DmD16-c3		17
wing disc ML-DmD21		22
wing disc ML-DmD32		15
haltere disc ML-DmD17-c3		16
eye-antennal disc ML-DmD11		15
antennal disc ML-DmD20-c5		19
mixed discs ML-DmD4-c1		14
CNS ML-DmBG1-c1		16
CNS ML-DmBG2-c2		23
tumorous blood cells mbn2		15
ovary fGS/OSS		22
ovary OSC		14
ovary OSS		9
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		16
Schneider line 2 Sg4		12
embryonic 1182-4H		16
embryonic GM2		22
embryonic Kc167		17
embryonic S1		20
embryonic S3		15
leg disc CME L1		16
wing disc CME-W2		13
wing disc ML-DmD8		11
wing disc ML-DmD9		16
wing disc ML-DmD16-c3		17
wing disc ML-DmD21		22
wing disc ML-DmD32		15
haltere disc ML-DmD17-c3		16
eye-antennal disc ML-DmD11		15
antennal disc ML-DmD20-c5		19
mixed discs ML-DmD4-c1		14
CNS ML-DmBG1-c1		16
CNS ML-DmBG2-c2		23
tumorous blood cells mbn2		15
ovary fGS/OSS		22
ovary OSC		14
ovary OSS		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		16
Schneider line 2 Sg4		12
embryonic 1182-4H		16
embryonic GM2		22
embryonic Kc167		17
embryonic S1		20
embryonic S3		15
leg disc CME L1		16
wing disc CME-W2		13
wing disc ML-DmD8		11
wing disc ML-DmD9		16
wing disc ML-DmD16-c3		17
wing disc ML-DmD21		22
wing disc ML-DmD32		15
haltere disc ML-DmD17-c3		16
eye-antennal disc ML-DmD11		15
antennal disc ML-DmD20-c5		19
mixed discs ML-DmD4-c1		14
CNS ML-DmBG1-c1		16
CNS ML-DmBG2-c2		23
tumorous blood cells mbn2		15
ovary fGS/OSS		22
ovary OSC		14
ovary OSS		9
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		16
Schneider line 2 Sg4		12
embryonic 1182-4H		16
embryonic GM2		22
embryonic Kc167		17
embryonic S1		20
embryonic S3		15
leg disc CME L1		16
wing disc CME-W2		13
wing disc ML-DmD8		11
wing disc ML-DmD9		16
wing disc ML-DmD16-c3		17
wing disc ML-DmD21		22
wing disc ML-DmD32		15
haltere disc ML-DmD17-c3		16
eye-antennal disc ML-DmD11		15
antennal disc ML-DmD20-c5		19
mixed discs ML-DmD4-c1		14
CNS ML-DmBG1-c1		16
CNS ML-DmBG2-c2		23
tumorous blood cells mbn2		15
ovary fGS/OSS		22
ovary OSC		14
ovary OSS		9
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		16
Schneider line 2 Sg4		12
embryonic 1182-4H		16
embryonic GM2		22
embryonic Kc167		17
embryonic S1		20
embryonic S3		15
leg disc CME L1		16
wing disc CME-W2		13
wing disc ML-DmD8		11
wing disc ML-DmD9		16
wing disc ML-DmD16-c3		17
wing disc ML-DmD21		22
wing disc ML-DmD32		15
haltere disc ML-DmD17-c3		16
eye-antennal disc ML-DmD11		15
antennal disc ML-DmD20-c5		19
mixed discs ML-DmD4-c1		14
CNS ML-DmBG1-c1		16
CNS ML-DmBG2-c2		23
tumorous blood cells mbn2		15
ovary fGS/OSS		22
ovary OSC		14
ovary OSS		9
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		16
Schneider line 2 Sg4		12
embryonic 1182-4H		16
embryonic GM2		22
embryonic Kc167		17
embryonic S1		20
embryonic S3		15
leg disc CME L1		16
wing disc CME-W2		13
wing disc ML-DmD8		11
wing disc ML-DmD9		16
wing disc ML-DmD16-c3		17
wing disc ML-DmD21		22
wing disc ML-DmD32		15
haltere disc ML-DmD17-c3		16
eye-antennal disc ML-DmD11		15
antennal disc ML-DmD20-c5		19
mixed discs ML-DmD4-c1		14
CNS ML-DmBG1-c1		16
CNS ML-DmBG2-c2		23
tumorous blood cells mbn2		15
ovary fGS/OSS		22
ovary OSC		14
ovary OSS		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		16
Schneider line 2 Sg4		12
embryonic 1182-4H		16
embryonic GM2		22
embryonic Kc167		17
embryonic S1		20
embryonic S3		15
leg disc CME L1		16
wing disc CME-W2		13
wing disc ML-DmD8		11
wing disc ML-DmD9		16
wing disc ML-DmD16-c3		17
wing disc ML-DmD21		22
wing disc ML-DmD32		15
haltere disc ML-DmD17-c3		16
eye-antennal disc ML-DmD11		15
antennal disc ML-DmD20-c5		19
mixed discs ML-DmD4-c1		14
CNS ML-DmBG1-c1		16
CNS ML-DmBG2-c2		23
tumorous blood cells mbn2		15
ovary fGS/OSS		22
ovary OSC		14
ovary OSS		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		11
cold shock, 4-day adult		10
heat shock, 4-day adult		15
Cadmium 50 mM 6 hrs, larvae L3		10
Cadmium 50 mM 12 hrs, larvae L3		8
Cadmium 50 mM 48 hrs, 4-day adult		15
Cadmium 100 mM 48 hrs, 4-day adult		26
Copper 0.5 mM 12 hrs, larvae L3		4
Copper 15 mM 48 hrs, 4-day adult		15
Zinc 5 mM 12 hrs, larvae L3		6
Zinc 4.5 mM 48 hrs, 4-day adult		17
Ethanol 2.5% 3 hrs, larvae L3		29
Ethanol 5% 3 hrs, larvae L3		13
Ethanol 10% 3 hrs, larvae L3		10
Caffeine 1.5 mg/ml 4 hrs, larvae L3		13
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		22
Caffeine 25 mg/ml 48 hrs, 4-day adult		10
Paraquat 5 mM 48 hrs, 4-day adult		27
Paraquat 10 mM 48 hrs, 4-day adult		31
Rotenone 2 μg 12 hrs, larvae L3		9
Rotenone 8 μg 12 hrs, larvae L3		11
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		11
cold shock, 4-day adult		10
heat shock, 4-day adult		15
Cadmium 50 mM 6 hrs, larvae L3		10
Cadmium 50 mM 12 hrs, larvae L3		8
Cadmium 50 mM 48 hrs, 4-day adult		15
Cadmium 100 mM 48 hrs, 4-day adult		26
Copper 0.5 mM 12 hrs, larvae L3		4
Copper 15 mM 48 hrs, 4-day adult		15
Zinc 5 mM 12 hrs, larvae L3		6
Zinc 4.5 mM 48 hrs, 4-day adult		17
Ethanol 2.5% 3 hrs, larvae L3		(29)
Ethanol 5% 3 hrs, larvae L3		13
Ethanol 10% 3 hrs, larvae L3		10
Caffeine 1.5 mg/ml 4 hrs, larvae L3		13
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		22
Caffeine 25 mg/ml 48 hrs, 4-day adult		10
Paraquat 5 mM 48 hrs, 4-day adult		(27)
Paraquat 10 mM 48 hrs, 4-day adult		(31)
Rotenone 2 μg 12 hrs, larvae L3		9
Rotenone 8 μg 12 hrs, larvae L3		11
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		11
cold shock, 4-day adult		10
heat shock, 4-day adult		15
Cadmium 50 mM 6 hrs, larvae L3		10
Cadmium 50 mM 12 hrs, larvae L3		8
Cadmium 50 mM 48 hrs, 4-day adult		15
Cadmium 100 mM 48 hrs, 4-day adult		26
Copper 0.5 mM 12 hrs, larvae L3		4
Copper 15 mM 48 hrs, 4-day adult		15
Zinc 5 mM 12 hrs, larvae L3		6
Zinc 4.5 mM 48 hrs, 4-day adult		17
Ethanol 2.5% 3 hrs, larvae L3		29
Ethanol 5% 3 hrs, larvae L3		13
Ethanol 10% 3 hrs, larvae L3		10
Caffeine 1.5 mg/ml 4 hrs, larvae L3		13
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		22
Caffeine 25 mg/ml 48 hrs, 4-day adult		10
Paraquat 5 mM 48 hrs, 4-day adult		27
Paraquat 10 mM 48 hrs, 4-day adult		31
Rotenone 2 μg 12 hrs, larvae L3		9
Rotenone 8 μg 12 hrs, larvae L3		11
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		11
cold shock, 4-day adult		10
heat shock, 4-day adult		15
Cadmium 50 mM 6 hrs, larvae L3		10
Cadmium 50 mM 12 hrs, larvae L3		8
Cadmium 50 mM 48 hrs, 4-day adult		15
Cadmium 100 mM 48 hrs, 4-day adult		26
Copper 0.5 mM 12 hrs, larvae L3		4
Copper 15 mM 48 hrs, 4-day adult		15
Zinc 5 mM 12 hrs, larvae L3		6
Zinc 4.5 mM 48 hrs, 4-day adult		17
Ethanol 2.5% 3 hrs, larvae L3		29
Ethanol 5% 3 hrs, larvae L3		13
Ethanol 10% 3 hrs, larvae L3		10
Caffeine 1.5 mg/ml 4 hrs, larvae L3		13
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		22
Caffeine 25 mg/ml 48 hrs, 4-day adult		10
Paraquat 5 mM 48 hrs, 4-day adult		27
Paraquat 10 mM 48 hrs, 4-day adult		31
Rotenone 2 μg 12 hrs, larvae L3		9
Rotenone 8 μg 12 hrs, larvae L3		11
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		11
cold shock, 4-day adult		10
heat shock, 4-day adult		15
Cadmium 50 mM 6 hrs, larvae L3		10
Cadmium 50 mM 12 hrs, larvae L3		8
Cadmium 50 mM 48 hrs, 4-day adult		15
Cadmium 100 mM 48 hrs, 4-day adult		26
Copper 0.5 mM 12 hrs, larvae L3		4
Copper 15 mM 48 hrs, 4-day adult		15
Zinc 5 mM 12 hrs, larvae L3		6
Zinc 4.5 mM 48 hrs, 4-day adult		17
Ethanol 2.5% 3 hrs, larvae L3		29
Ethanol 5% 3 hrs, larvae L3		13
Ethanol 10% 3 hrs, larvae L3		10
Caffeine 1.5 mg/ml 4 hrs, larvae L3		13
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		22
Caffeine 25 mg/ml 48 hrs, 4-day adult		10
Paraquat 5 mM 48 hrs, 4-day adult		27
Paraquat 10 mM 48 hrs, 4-day adult		31
Rotenone 2 μg 12 hrs, larvae L3		9
Rotenone 8 μg 12 hrs, larvae L3		11
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		11
cold shock, 4-day adult		10
heat shock, 4-day adult		15
Cadmium 50 mM 6 hrs, larvae L3		10
Cadmium 50 mM 12 hrs, larvae L3		8
Cadmium 50 mM 48 hrs, 4-day adult		15
Cadmium 100 mM 48 hrs, 4-day adult		26
Copper 0.5 mM 12 hrs, larvae L3		4
Copper 15 mM 48 hrs, 4-day adult		15
Zinc 5 mM 12 hrs, larvae L3		6
Zinc 4.5 mM 48 hrs, 4-day adult		17
Ethanol 2.5% 3 hrs, larvae L3		29
Ethanol 5% 3 hrs, larvae L3		13
Ethanol 10% 3 hrs, larvae L3		10
Caffeine 1.5 mg/ml 4 hrs, larvae L3		13
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		22
Caffeine 25 mg/ml 48 hrs, 4-day adult		10
Paraquat 5 mM 48 hrs, 4-day adult		27
Paraquat 10 mM 48 hrs, 4-day adult		31
Rotenone 2 μg 12 hrs, larvae L3		9
Rotenone 8 μg 12 hrs, larvae L3		11
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		11
cold shock, 4-day adult		10
heat shock, 4-day adult		15
Cadmium 50 mM 6 hrs, larvae L3		10
Cadmium 50 mM 12 hrs, larvae L3		8
Cadmium 50 mM 48 hrs, 4-day adult		15
Cadmium 100 mM 48 hrs, 4-day adult		26
Copper 0.5 mM 12 hrs, larvae L3		4
Copper 15 mM 48 hrs, 4-day adult		15
Zinc 5 mM 12 hrs, larvae L3		6
Zinc 4.5 mM 48 hrs, 4-day adult		17
Ethanol 2.5% 3 hrs, larvae L3		29
Ethanol 5% 3 hrs, larvae L3		13
Ethanol 10% 3 hrs, larvae L3		10
Caffeine 1.5 mg/ml 4 hrs, larvae L3		13
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		22
Caffeine 25 mg/ml 48 hrs, 4-day adult		10
Paraquat 5 mM 48 hrs, 4-day adult		27
Paraquat 10 mM 48 hrs, 4-day adult		31
Rotenone 2 μg 12 hrs, larvae L3		9
Rotenone 8 μg 12 hrs, larvae L3		11
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		11
cold shock, 4-day adult		10
heat shock, 4-day adult		15
Cadmium 50 mM 6 hrs, larvae L3		10
Cadmium 50 mM 12 hrs, larvae L3		8
Cadmium 50 mM 48 hrs, 4-day adult		15
Cadmium 100 mM 48 hrs, 4-day adult		26
Copper 0.5 mM 12 hrs, larvae L3		4
Copper 15 mM 48 hrs, 4-day adult		15
Zinc 5 mM 12 hrs, larvae L3		6
Zinc 4.5 mM 48 hrs, 4-day adult		17
Ethanol 2.5% 3 hrs, larvae L3		29
Ethanol 5% 3 hrs, larvae L3		13
Ethanol 10% 3 hrs, larvae L3		10
Caffeine 1.5 mg/ml 4 hrs, larvae L3		13
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		22
Caffeine 25 mg/ml 48 hrs, 4-day adult		10
Paraquat 5 mM 48 hrs, 4-day adult		27
Paraquat 10 mM 48 hrs, 4-day adult		31
Rotenone 2 μg 12 hrs, larvae L3		9
Rotenone 8 μg 12 hrs, larvae L3		11
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010, Graveley et al., 2011)

External Data & Images

Linkouts

BDGP expression data

•

LD03322

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0086784

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal (with stmA^1-2)

stmA¹²

lethal (with stmA^1-4)

stmA¹²

lethal (with stmA^2-2)

stmA¹²

lethal (with stmA¹²)

lethal (with stmA^18-2)

stmA¹²

lethal (with stmA^27-1)

stmA¹²

lethal (with stmA^PEL1)

stmA¹²

lethal (with stmA^PEL3)

stmA¹²

lethal (with stmA^PEL4)

stmA¹²

lethal (with stmA^PEL6)

stmA¹²

lethal (with stmA^PEL8)

stmA¹²

lethal (with stmA^PEL11)

stmA¹²

lethal | embryonic stage

lethal | embryonic stage | recessive

lethal | first instar larval stage

stmA^{S358A.T:Avic\GFP-EGFP}

lethal | heat sensitive

lethal | maternal effect

lethal | pupal stage, with Scer\GAL4^pnr-MD237

stmA^GD14013

lethal | recessive

lethal | semidominant

viable

viable, with stmA¹, stmA^rev499

viable, with stmA¹, stmA^{S358A.T:Avic\GFP-EGFP}

stmA^rev499

viable, with stmA¹, stmA^{T:Avic\GFP-EGFP}

stmA^rev499

viable, with stmA¹/stmA^rev499

stmA^{S358A.T:Avic\GFP-EGFP}

viable, with stmA^{S358A.T:Avic\GFP-EGFP}, stmA^rev499

stmA¹

viable, with stmA^{S358A.T:Avic\GFP-EGFP}/stmA^rev499

stmA¹

viable, with stmA^{T:Avic\GFP-EGFP}, stmA^rev499

stmA¹

viable (with stmA¹), with stmA^{S358A.T:Avic\GFP-EGFP}

stmA^rev499

viable (with stmA^rev499), with stmA¹

stmA^{S358A.T:Avic\GFP-EGFP}

viable (with stmA^rev499), with stmA^{S358A.T:Avic\GFP-EGFP}

stmA¹

viable (with stmA^{S358A.T:Avic\GFP-EGFP}), with stmA¹

stmA^rev499

Other Phenotypes

Allele

stmA²

hypoactive

neurophysiology defective | heat sensitive

stmA¹

neurophysiology defective | heat sensitive (with stmA¹)

stmA^rev499

neurophysiology defective | heat sensitive (with stmA^rev499)

stmA¹

paralytic | adult stage | semidominant

paralytic | dominant

paralytic | dominant | heat sensitive

paralytic | heat sensitive

paralytic | heat sensitive (with Df(2R)H3C1)

paralytic | heat sensitive (with Df(2R)H3D3)

stmA¹

paralytic | heat sensitive (with Df(2R)H3E1)

paralytic | heat sensitive (with stmA¹)

paralytic | heat sensitive (with stmA²)

stmA¹

paralytic | heat sensitive (with stmA⁷)

stmA¹

paralytic | heat sensitive (with stmA¹²)

stmA¹

paralytic | heat sensitive (with stmA^18-2)

stmA¹

paralytic | heat sensitive (with stmA^KP1)

stmA¹

paralytic | heat sensitive (with stmA^KP2)

stmA¹

paralytic | heat sensitive (with stmA^KP3)

stmA¹

paralytic | heat sensitive (with stmA^KP4)

stmA¹

paralytic | heat sensitive (with stmA^KP5)

stmA¹

paralytic | heat sensitive (with stmA^KP6)

stmA¹

paralytic | heat sensitive (with stmA^p1)

stmA¹

paralytic | heat sensitive (with stmA^p2)

stmA¹

paralytic | heat sensitive (with stmA^p3)

stmA¹

paralytic | heat sensitive (with stmA^p4)

stmA¹

paralytic | heat sensitive (with stmA^p5)

stmA¹

paralytic | heat sensitive (with stmA^PEL1)

stmA¹

paralytic | heat sensitive (with stmA^PEL3)

stmA¹

paralytic | heat sensitive (with stmA^PEL4)

stmA¹

paralytic | heat sensitive (with stmA^PEL6)

stmA¹

paralytic | heat sensitive (with stmA^PEL8)

stmA¹

paralytic | heat sensitive (with stmA^PEL11)

stmA¹

paralytic | heat sensitive (with stmA^rev499)

stmA¹

paralytic | larval stage | semidominant

paralytic | recessive | heat sensitive

stmA¹

visible | recessive

wild-type

Phenotype manifest in

Allele

amnioserosa

antenno-maxillary complex

stmA¹

cephalopharyngeal skeleton | embryonic stage

cuticle | anterior | dorsal

denticle belt | embryonic stage

dorsal medial muscle

stmA¹

ectoderm

embryonic/larval brain | embryonic stage

embryonic/larval foregut | embryonic stage

embryonic/larval neuromuscular junction

embryonic/larval neuromuscular junction (with stmA¹)

stmA^rev499

embryonic/larval neuromuscular junction (with stmA^rev499)

stmA¹

embryonic/larval pharynx | embryonic stage

embryonic epidermis

extended germ band embryo

eye

garland cell | wandering third instar larval stage | temperature conditional

stmA¹

head

labral segment

leg

mesothoracic extracoxal depressor muscle 66

stmA¹

NMJ bouton | wandering third instar larval stage | temperature conditional

stmA¹

presynaptic active zone membrane

stmA¹

proctodeum

retina | heat sensitive (with stmA¹)

stmA^rev499

retina | heat sensitive (with stmA^rev499)

stmA¹

subesophageal ganglion

stmA¹

synaptic vesicle

stmA¹

terminal bouton

stmA¹

ventral nerve cord

Classical Alleles ( 44 )

For All Classical Alleles Show

Allele of stmA	Class	Mutagen	Stocks	Known lesion
stmA^cmp44E-1		ethyl methanesulfonate	2	--
stmA^KG03925		P-element activity	2	--
stmA^18-2		gamma ray	1	Yes
stmA¹	heat sensitive neomorphic allele - genetic evidence		1	Yes
stmA²	heat sensitive neomorphic allele - genetic evidence	ethyl methanesulfonate	1	--
stmA^e00058		piggyBac transposase	1	--
stmA^EY09152		P-element activity	1	--
stmA^f03887		piggyBac transposase	1	--
stmA^MI05288			1	--
stmA^NP0882			1	--
stmA^1-2		gamma ray	0	--
stmA^1-4		gamma ray	0	--
stmA¹²		ethyl methanesulfonate	0	--
stmA^2-2		gamma ray	0	--
stmA^27-1		gamma ray	0	--
stmA⁷		ethyl methanesulfonate	0	--
stmA^cmp44E-3		ethyl methanesulfonate	0	--
stmA^cmp44E-4		ethyl methanesulfonate	0	--
stmA^cmp44E-5		ethyl methanesulfonate	0	--
stmA^KP1		ethyl methanesulfonate	0	--
stmA^KP2		ethyl methanesulfonate	0	--
stmA^KP3		ethyl methanesulfonate	0	--
stmA^KP4		ethyl methanesulfonate	0	--
stmA^KP5		ethyl methanesulfonate	0	--
stmA^KP6		ethyl methanesulfonate	0	--
stmA^p1		Delta2-3	0	--
stmA^p2		Delta2-3	0	--
stmA^p3		Delta2-3	0	--
stmA^p4		Delta2-3	0	Yes
stmA^p5		Delta2-3	0	--
stmA^PEL11		Delta2-3	0	Yes
stmA^PEL1		Delta2-3	0	Yes
stmA^PEL3		Delta2-3	0	Yes
stmA^PEL4		Delta2-3	0	Yes
stmA^PEL6		Delta2-3	0	Yes
stmA^PEL8		Delta2-3	0	Yes
stmA^pngcl		P-element activity	0	Yes
stmA^rbo-P1		P-element activity	0	Yes
stmA^rbo-P2		P-element activity	0	Yes
stmA^rev499	loss of function allele	Delta2-3	0	Yes
stmA^rev522		Delta2-3	0	Yes
stmA^rev787		Delta2-3	0	Yes
stmA^rev792		Delta2-3	0	Yes
stmA^rev828		Delta2-3	0	Yes

Alleles Carried on Transgenic Constructs ( 9 )

For All Alleles Carried on Transgenic Constructs Show

Allele of stmA	Mutagen	Stocks	Known lesion
stmA^GD14013	in vitro construct - RNAi in vitro construct - regulatory fusion	4	Yes
stmA^HMS01982	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
stmA^KK100493	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
stmA^+t17	in vitro construct - genomic fragment	0	Yes
stmA^+t8.5	in vitro construct - genomic fragment	0	Yes
stmA^{MtnA.T:SV5\V5}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	0	Yes
stmA^NIG.8739R	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes
stmA^{S358A.T:Avic\GFP-EGFP}	in vitro construct - genomic fragment in vitro construct - coding region fusion in vitro construct - site directed mutagenesis	0	Yes
stmA^{T:Avic\GFP-EGFP}	in vitro construct - coding region fusion	0	Yes

Aneuploid Aberrations

Disrupted in

Df(2R)BSC268

(Christensen et al., 2008.12.28)

Df(2R)BSC269

(Christensen and Cook, 2007.3.22, Christensen and Cook, 2007.3.22)

Df(2R)cmp44E-rev390

(Faulkner et al., 1998, Robertson et al., 1999)

Df(2R)H3C1

(Kumar et al., 2001, Huang et al., 2004)

Df(2R)H3D3

(Huang et al., 2004, Huang et al., 2006)

Df(2R)H3E1

(Rao et al., 2001, Kumar et al., 2001, Dockendorff et al., 2000)

Df(2R)Np3

(Huang et al., 2004)

Partially disrupted in

Df(2R)H3C1

(Rao et al., 2001)

Not disrupted in

Df(2R)42

(Chandrashekaran and Sarla, 1993)

Df(2R)44CE

(Kumar et al., 2001)

Df(2R)cn9

(Kumar et al., 2001)

Df(2R)cn87e

(Kumar et al., 2001)

Df(2R)pk78k

(Chandrashekaran and Sarla, 1993)

Df(2R)pk78s

(Chandrashekaran and Sarla, 1993)

Df(2R)rl10a

(Chandrashekaran and Sarla, 1993)

Df(2R)rl10b

(Chandrashekaran and Sarla, 1993)

Df(2R)ST1

(Chandrashekaran and Sarla, 1993)

Df(2R)V30

(Chandrashekaran and Sarla, 1993)

In(2R)D5^LTE146-sz4^R

(Chandrashekaran and Sarla, 1993)

Duplicated in

Dp(2;3)P32

(Chandrashekaran and Sarla, 1993)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

characterization construct

P{stmA-EGFP.H}

P{stmA-EGFP.S358A}

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{EP}EP2233

P{EPgy2}stmA^EY09152

P{GSV1}EP-405^EP-405

insertion of enhancer trap

P{lacW}l(2)SH0382^SH0382

P{lacW}stmA^pngcl

P{lacW}stmA^rbo-P1

P{lacW}stmA^rbo-P2

insertion of enhancer trap binary system

P{GawB}stmA^NP0882

miscellaneous insertions

Mi{MIC}stmA^MI05288

P{}stmA^p1

P{}stmA^p4

P{SUPor-P}stmA^KG03925

PBac{RB}stmA^e00058

PBac{WH}stmA^f03887

Gene Ontology: Function, Process & Cellular Component ( 8 unique terms )

Terms Based on Experimental Evidence ( 7 terms )

Molecular Function ( 0 terms)

Biological Process

CV term

Evidence

References

cytokinesis