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Gene Dmel\tlk

General Information
Symbol	Dmel\tlk	Species	D. melanogaster
Name	Tousled-like kinase	Annotation symbol	CG34412
Feature type	protein_coding_gene	FlyBase ID	FBgn0086899
Gene Model Status	Current	Stock availability	69 publicly available
Also Known As	CG2829, CG32782, l(1)G0054, CG12462
Genomic Location
Chromosome (arm)	X	Recombination map
Cytogenetic map	3F1-3E7	Sequence location	X:3,614,054..3,682,851 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Tousled-like kinase is referred to in FlyBase by the symbol Dmel\tlk (CG34412, FBgn0086899). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding; protein kinase activity. There is experimental evidence that it is involved in the biological process: regulation of cell shape; protein autophosphorylation; mitotic cell cycle, embryonic; chromatin organization; chromosome segregation; protein phosphorylation; cell adhesion; histone phosphorylation; antimicrobial humoral response; regulation of cell cycle. 91 alleles are reported. The phenotypes of these alleles are annotated with: eye; macrochaeta; embryonic/larval salivary gland; head. It has 8 annotated transcripts and 8 annotated polypeptides. Protein features are: Protein kinase, ATP binding site; Serine/threonine-protein kinase-like domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: No expression detected in any larval or adult organs/tissues. Comments on Affy2 ProbeSet: ProbeSet 1633368_at partially aligns to an exonic region of only a subset of 1 of the 8 FlyBase-annotated transcript isoforms of tlk: 6 out of 14 probes fall into this exonic region. Gene sequence location is X:3614054..3682851.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	References Knowles-Barley, 2011.8.24, BrainTrap expression curation.
FB2012_01	References Reis et al., 2010, PLoS Genet. 6(11): e1001206 Sequence features BKN31248 BKN50352 BKN50260 BKN28036 HFA17835 HFA17731 HFA17281
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 3F1-3E7   Limits computationally determined from genome sequence between P{EP}EP1200EP1200&P{EP}EP1343 and P{EP}CG2930EP1352 3F1--5; Limits computationally determined from genome sequence between P{EP}EP1200EP1200&P{EP}EP1343 and P{EP}CG2930EP1352
Experimentally Determined Cytological Location
Cytogenetic map Notes References 3F4-3F5   (determined by in situ hybridisation)   (Peter et al., 2002) 3F1-3F5   (determined by in situ hybridisation)   (Schaefer et al., 1999.6.15) 3F4-3F5   (determined by in situ hybridisation)   (Schaefer et al., 1999.3.29)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\tlk for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	3' UTR contains an ORF that is conserved among close Drosophila species (potential peptide 129 aa in length). Gene merge based on EST/cDNA data. Due to significant sequence change in this region between Releases 4 and 5, data that predate Release 5 are not shown for some categories of evidence.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) tlk-RB FBtr0112680  NM_001103403   4624   1463 tlk-RC FBtr0070596     5849   1254 tlk-RE FBtr0299579  NM_206620   5537   1255 tlk-RF FBtr0299580  NM_001144680   6499   1266 tlk-RG FBtr0299581  NM_001144681   7243   1489 tlk-RH FBtr0299582  NM_001144682   5876   1266 tlk-RI FBtr0299583  NM_001144683   4673   1242 tlk-RJ FBtr0301659  NM_001169184   4633   1266
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein tlk-PB   FBpp0111592   160.3   1463   9.10     NP_001096873   AAN09103 tlk-PC   FBpp0099673   135.5   1254   9.31       AAN09104 tlk-PE   FBpp0288854   135.6   1255   9.36     NP_996343   AAS72337 tlk-PF   FBpp0288855   136.7   1266   9.10     NP_001138152   ACL82885 tlk-PG   FBpp0288856   162.2   1489   9.62     NP_001138153   ACL82886 tlk-PH   FBpp0288857   136.7   1266   9.10     NP_001138154   ACL82887 tlk-PI   FBpp0288858   133.9   1242   9.02     NP_001138155   ACL82888 tlk-PJ   FBpp0290873   136.7   1266   9.10     NP_001162655   ACZ95192
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, ATP binding site (IPR017441) Serine/threonine-protein kinase-like domain (IPR017442)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA141350   AA201800   AA201997   AA202484   AA264210   AA391297   AA438516   AA438857   AA440473   AA440573   AA441301   AA536326   AA539237   AA540118   AA540453   AA696731   AA697276   AA697944   AA801847   AA816249   AA816850   AA949425   AA949694   AE014298 AAN09103   AE014298 AAN09104   AE014298 AAS72337   AE014298 ACL82885   AE014298 ACL82886   AE014298 ACL82887   AE014298 ACL82888   AE014298 ACZ95192   AF181637   AI113830   AI455055   AI514458   AI515370   AI515499   AI516006   AI516066   AI518165   AI518878   AI519314   AI531615   AI546568   AQ025450   AW941751   BF505227   BG638013   BG638179   BG639212   BI165256   BI352578   BI355184   BI375874   BI568660   BI582042   BI592336   BI592818   BI616535   BI620714   BI640042   BP540580   BP552969   BP555953   BT014926   BT024217   BT025041   BT029136   BT030440   BT120061   CO156406   CO177690   CO179630   CO179636   CO189472   CO262170   CO264436   CO264697   CO266895   CO270463   CO271527   CO273820   CO277242   CO278314   CO278959   CO280221   CO280237   CO282305   CO284611   CO285575   CO295005   CO298598   CO298669   CO300778   CO301443   CO303768   CO304558   CO307180   CO308023   CO313535   CO313863   CO315689   CO316938   CO321093   CO330668   CO331138   CO334131   CO337742   CO339339   CO344692   DQ327746   EC053037   EC062783   EC066198   EC069317   EC072419   EC076736   EC079572   EC084054   EC084187   EC084766   EC088630   EC088937   EC194797   EC205687   EC207122   EC212916   EC216334   EC218007   EC219764   EC220603   EC222000   EC226906   EC230411   EC231010   EC237209   EC237384   EC241853   EC242467   EC242928   EC243468   EC243895   EC244456   EC244569   EC248171   EC256335   EC256822   EC265236   EC265785   EV581728
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q8IRT8 B7Z126 B7Z120 Q1RL19
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 3 -- 9   organism | ubiquitous   (Carrera et al., 2003) larval stage   imaginal disc   (Carrera et al., 2003) adult stage | female & oogenesis stage S10   nurse cell & oocyte   (Carrera et al., 2003) Comment:maternal transcript transported from nurse cells to oocytes northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage 3     (Carrera et al., 2003)
Additional Descriptive Data
	tlk is ubiquituously distributed in all proliferative tissues. (Carrera et al., 2003)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage 5 -- 8   cell   • nucleus (Carrera et al., 2003) Comment:interphase larval stage   salivary gland   • nucleus (Carrera et al., 2003) Comment:interphase western blot Stage Tissue/Position (including subcellular localization) Reference fertilized egg stage   egg   (Carrera et al., 2003) embryonic stage   organism   • nucleus (Carrera et al., 2003)
Additional Descriptive Data
	tlk protein is localized to the nucleus in interphase cells, and diffusely distributed throughout the cell during other parts of the mitotic cell cycle. tlk protein does not associate directly with DNA. (Carrera et al., 2003)
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0086899    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0086899 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0086899 expression level Developmental Stage   Expression Level embryo 00-02hr    6194 embryo 02-04hr    6830 embryo 04-06hr    2611 embryo 06-08hr    2510 embryo 08-10hr    2587 embryo 10-12hr    1895 embryo 12-14hr    1932 embryo 14-16hr    3154 embryo 16-18hr    2474 embryo 18-20hr    1594 embryo 20-22hr    3345 embryo 22-24hr    2611 larva L1    753 larva L2    480 larva L3 12hr old    501 larva L3 puffstage 1-2    342 larva L3 puffstage 3-6    1005 larva L3 puffstage 7-9    915 white prepupae new    940 white prepupae 12hr    1144 white prepupae 24hr    1586 pupae 2d postWPP    1156 pupae 3d postWPP    604 pupae 4d postWPP    642 adult male 01day    563 adult male 05day    642 adult male 30day    692 adult female 01day    1886 adult female 05day    2872 adult female 30day    3250 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (6194) embryo 02-04hr  (6830) embryo 04-06hr  (2611) embryo 06-08hr  (2510) embryo 08-10hr  (2587) embryo 10-12hr  (1895) embryo 12-14hr  (1932) embryo 14-16hr  (3154) embryo 16-18hr  (2474) embryo 18-20hr  (1594) embryo 20-22hr  (3345) embryo 22-24hr  (2611) larva L1  (753) larva L2  (480) larva L3 12hr old  (501) larva L3 puffstage 1-2  (342) larva L3 puffstage 3-6  (1005) larva L3 puffstage 7-9  (915) white prepupae new  (940) white prepupae 12hr  (1144) white prepupae 24hr  (1586) pupae 2d postWPP  (1156) pupae 3d postWPP  (604) pupae 4d postWPP  (642) adult male 01day  (563) adult male 05day  (642) adult male 30day  (692) adult female 01day  (1886) adult female 05day  (2872) adult female 30day  (3250) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (6194) embryo 02-04hr  (6830) embryo 04-06hr  (2611) embryo 06-08hr  (2510) embryo 08-10hr  (2587) embryo 10-12hr  (1895) embryo 12-14hr  (1932) embryo 14-16hr  (3154) embryo 16-18hr  (2474) embryo 18-20hr  (1594) embryo 20-22hr  (3345) embryo 22-24hr  (2611) larva L1    753 larva L2    480 larva L3 12hr old    501 larva L3 puffstage 1-2    342 larva L3 puffstage 3-6    1005 larva L3 puffstage 7-9    915 white prepupae new    940 white prepupae 12hr    1144 white prepupae 24hr  (1586) pupae 2d postWPP    1156 pupae 3d postWPP    604 pupae 4d postWPP    642 adult male 01day    563 adult male 05day    642 adult male 30day    692 adult female 01day  (1886) adult female 05day  (2872) adult female 30day  (3250) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    6194 embryo 02-04hr    6830 embryo 04-06hr    2611 embryo 06-08hr    2510 embryo 08-10hr    2587 embryo 10-12hr    1895 embryo 12-14hr    1932 embryo 14-16hr    3154 embryo 16-18hr    2474 embryo 18-20hr    1594 embryo 20-22hr    3345 embryo 22-24hr    2611 larva L1    753 larva L2    480 larva L3 12hr old    501 larva L3 puffstage 1-2    342 larva L3 puffstage 3-6    1005 larva L3 puffstage 7-9    915 white prepupae new    940 white prepupae 12hr    1144 white prepupae 24hr    1586 pupae 2d postWPP    1156 pupae 3d postWPP    604 pupae 4d postWPP    642 adult male 01day    563 adult male 05day    642 adult male 30day    692 adult female 01day    1886 adult female 05day    2872 adult female 30day    3250 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    6194 embryo 02-04hr    6830 embryo 04-06hr    2611 embryo 06-08hr    2510 embryo 08-10hr    2587 embryo 10-12hr    1895 embryo 12-14hr    1932 embryo 14-16hr    3154 embryo 16-18hr    2474 embryo 18-20hr    1594 embryo 20-22hr    3345 embryo 22-24hr    2611 larva L1    753 larva L2    480 larva L3 12hr old    501 larva L3 puffstage 1-2    342 larva L3 puffstage 3-6    1005 larva L3 puffstage 7-9    915 white prepupae new    940 white prepupae 12hr    1144 white prepupae 24hr    1586 pupae 2d postWPP    1156 pupae 3d postWPP    604 pupae 4d postWPP    642 adult male 01day    563 adult male 05day    642 adult male 30day    692 adult female 01day    1886 adult female 05day    2872 adult female 30day    3250 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0086899 expression level Developmental Stage   Expression Level embryo 00-02hr    6194 embryo 02-04hr    6830 embryo 04-06hr    2611 embryo 06-08hr    2510 embryo 08-10hr    2587 embryo 10-12hr    1895 embryo 12-14hr    1932 embryo 14-16hr    3154 embryo 16-18hr    2474 embryo 18-20hr    1594 embryo 20-22hr    3345 embryo 22-24hr    2611 larva L1    753 larva L2    480 larva L3 12hr old    501 larva L3 puffstage 1-2    342 larva L3 puffstage 3-6    1005 larva L3 puffstage 7-9    915 white prepupae new    940 white prepupae 12hr    1144 white prepupae 24hr    1586 pupae 2d postWPP    1156 pupae 3d postWPP    604 pupae 4d postWPP    642 adult male 01day    563 adult male 05day    642 adult male 30day    692 adult female 01day    1886 adult female 05day    2872 adult female 30day    3250 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (6194) embryo 02-04hr  (6830) embryo 04-06hr  (2611) embryo 06-08hr  (2510) embryo 08-10hr  (2587) embryo 10-12hr  (1895) embryo 12-14hr  (1932) embryo 14-16hr  (3154) embryo 16-18hr  (2474) embryo 18-20hr  (1594) embryo 20-22hr  (3345) embryo 22-24hr  (2611) larva L1  (753) larva L2  (480) larva L3 12hr old  (501) larva L3 puffstage 1-2  (342) larva L3 puffstage 3-6  (1005) larva L3 puffstage 7-9  (915) white prepupae new  (940) white prepupae 12hr  (1144) white prepupae 24hr  (1586) pupae 2d postWPP  (1156) pupae 3d postWPP  (604) pupae 4d postWPP  (642) adult male 01day  (563) adult male 05day  (642) adult male 30day  (692) adult female 01day  (1886) adult female 05day  (2872) adult female 30day  (3250) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (6194) embryo 02-04hr  (6830) embryo 04-06hr  (2611) embryo 06-08hr  (2510) embryo 08-10hr  (2587) embryo 10-12hr  1895 embryo 12-14hr  1932 embryo 14-16hr  (3154) embryo 16-18hr  (2474) embryo 18-20hr  1594 embryo 20-22hr  (3345) embryo 22-24hr  (2611) larva L1    753 larva L2    480 larva L3 12hr old    501 larva L3 puffstage 1-2    342 larva L3 puffstage 3-6    1005 larva L3 puffstage 7-9    915 white prepupae new    940 white prepupae 12hr    1144 white prepupae 24hr  1586 pupae 2d postWPP    1156 pupae 3d postWPP    604 pupae 4d postWPP    642 adult male 01day    563 adult male 05day    642 adult male 30day    692 adult female 01day  1886 adult female 05day  (2872) adult female 30day  (3250) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    6194 embryo 02-04hr    6830 embryo 04-06hr    2611 embryo 06-08hr    2510 embryo 08-10hr    2587 embryo 10-12hr    1895 embryo 12-14hr    1932 embryo 14-16hr    3154 embryo 16-18hr    2474 embryo 18-20hr    1594 embryo 20-22hr    3345 embryo 22-24hr    2611 larva L1    753 larva L2    480 larva L3 12hr old    501 larva L3 puffstage 1-2    342 larva L3 puffstage 3-6    1005 larva L3 puffstage 7-9    915 white prepupae new    940 white prepupae 12hr    1144 white prepupae 24hr    1586 pupae 2d postWPP    1156 pupae 3d postWPP    604 pupae 4d postWPP    642 adult male 01day    563 adult male 05day    642 adult male 30day    692 adult female 01day    1886 adult female 05day    2872 adult female 30day    3250 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    6194 embryo 02-04hr    6830 embryo 04-06hr    2611 embryo 06-08hr    2510 embryo 08-10hr    2587 embryo 10-12hr    1895 embryo 12-14hr    1932 embryo 14-16hr    3154 embryo 16-18hr    2474 embryo 18-20hr    1594 embryo 20-22hr    3345 embryo 22-24hr    2611 larva L1    753 larva L2    480 larva L3 12hr old    501 larva L3 puffstage 1-2    342 larva L3 puffstage 3-6    1005 larva L3 puffstage 7-9    915 white prepupae new    940 white prepupae 12hr    1144 white prepupae 24hr    1586 pupae 2d postWPP    1156 pupae 3d postWPP    604 pupae 4d postWPP    642 adult male 01day    563 adult male 05day    642 adult male 30day    692 adult female 01day    1886 adult female 05day    2872 adult female 30day    3250 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0086899    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0086899 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: No expression detected in any larval or adult organs/tissues. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0086899 expression level Tissue   Expression Level Larval Central Nervous System    5.875 Larval Midgut    1.5 Larval Hindgut    1.7 Larval Malpighian Tubules    3.9 Larval Fat Body    0.7 Larval Salivary Gland    1.8 Larval Trachea    3.375 Larval Carcass    2.975 Adult Head    2.1 Adult Eye    1.7 Adult Brain    4.5 Adult Thoracic-Abdominal Ganglion    1.5 Adult Crop    5 Adult Midgut    2.3 Adult Hindgut    2.2 Adult Malpighian Tubules    2.5 Adult Fat Body    4.6 Adult Salivary Gland    2.1 Adult Heart    1.525 Adult VirginFemale Spermatheca    0.4 Adult InseminatedFemale Spermatheca    0.9 Adult Ovary    0.8 Adult Testis    0.9 Adult Male Accessory Gland    5.3 Adult Carcass    5.8 Expression Level Scale  None   Low  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    5.875 Larval Midgut    1.5 Larval Hindgut    1.7 Larval Malpighian Tubules    3.9 Larval Fat Body    0.7 Larval Salivary Gland    1.8 Larval Trachea    3.375 Larval Carcass    2.975 Adult Head    2.1 Adult Eye    1.7 Adult Brain    4.5 Adult Thoracic-Abdominal Ganglion    1.5 Adult Crop    5 Adult Midgut    2.3 Adult Hindgut    2.2 Adult Malpighian Tubules    2.5 Adult Fat Body    4.6 Adult Salivary Gland    2.1 Adult Heart    1.525 Adult VirginFemale Spermatheca    0.4 Adult InseminatedFemale Spermatheca    0.9 Adult Ovary    0.8 Adult Testis    0.9 Adult Male Accessory Gland    5.3 Adult Carcass    5.8 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    5.875 Larval Midgut    1.5 Larval Hindgut    1.7 Larval Malpighian Tubules    3.9 Larval Fat Body    0.7 Larval Salivary Gland    1.8 Larval Trachea    3.375 Larval Carcass    2.975 Adult Head    2.1 Adult Eye    1.7 Adult Brain    4.5 Adult Thoracic-Abdominal Ganglion    1.5 Adult Crop    5 Adult Midgut    2.3 Adult Hindgut    2.2 Adult Malpighian Tubules    2.5 Adult Fat Body    4.6 Adult Salivary Gland    2.1 Adult Heart    1.525 Adult VirginFemale Spermatheca    0.4 Adult InseminatedFemale Spermatheca    0.9 Adult Ovary    0.8 Adult Testis    0.9 Adult Male Accessory Gland    5.3 Adult Carcass    5.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    5.875 Larval Midgut    1.5 Larval Hindgut    1.7 Larval Malpighian Tubules    3.9 Larval Fat Body    0.7 Larval Salivary Gland    1.8 Larval Trachea    3.375 Larval Carcass    2.975 Adult Head    2.1 Adult Eye    1.7 Adult Brain    4.5 Adult Thoracic-Abdominal Ganglion    1.5 Adult Crop    5 Adult Midgut    2.3 Adult Hindgut    2.2 Adult Malpighian Tubules    2.5 Adult Fat Body    4.6 Adult Salivary Gland    2.1 Adult Heart    1.525 Adult VirginFemale Spermatheca    0.4 Adult InseminatedFemale Spermatheca    0.9 Adult Ovary    0.8 Adult Testis    0.9 Adult Male Accessory Gland    5.3 Adult Carcass    5.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0086899 expression level Tissue   Expression Level Larval Central Nervous System    5.875 Larval Midgut    1.5 Larval Hindgut    1.7 Larval Malpighian Tubules    3.9 Larval Fat Body    0.7 Larval Salivary Gland    1.8 Larval Trachea    3.375 Larval Carcass    2.975 Adult Head    2.1 Adult Eye    1.7 Adult Brain    4.5 Adult Thoracic-Abdominal Ganglion    1.5 Adult Crop    5 Adult Midgut    2.3 Adult Hindgut    2.2 Adult Malpighian Tubules    2.5 Adult Fat Body    4.6 Adult Salivary Gland    2.1 Adult Heart    1.525 Adult VirginFemale Spermatheca    0.4 Adult InseminatedFemale Spermatheca    0.9 Adult Ovary    0.8 Adult Testis    0.9 Adult Male Accessory Gland    5.3 Adult Carcass    5.8 Expression Level Scale  None   Low  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    5.875 Larval Midgut    1.5 Larval Hindgut    1.7 Larval Malpighian Tubules    3.9 Larval Fat Body    0.7 Larval Salivary Gland    1.8 Larval Trachea    3.375 Larval Carcass    2.975 Adult Head    2.1 Adult Eye    1.7 Adult Brain    4.5 Adult Thoracic-Abdominal Ganglion    1.5 Adult Crop    5 Adult Midgut    2.3 Adult Hindgut    2.2 Adult Malpighian Tubules    2.5 Adult Fat Body    4.6 Adult Salivary Gland    2.1 Adult Heart    1.525 Adult VirginFemale Spermatheca    0.4 Adult InseminatedFemale Spermatheca    0.9 Adult Ovary    0.8 Adult Testis    0.9 Adult Male Accessory Gland    5.3 Adult Carcass    5.8 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    5.875 Larval Midgut    1.5 Larval Hindgut    1.7 Larval Malpighian Tubules    3.9 Larval Fat Body    0.7 Larval Salivary Gland    1.8 Larval Trachea    3.375 Larval Carcass    2.975 Adult Head    2.1 Adult Eye    1.7 Adult Brain    4.5 Adult Thoracic-Abdominal Ganglion    1.5 Adult Crop    5 Adult Midgut    2.3 Adult Hindgut    2.2 Adult Malpighian Tubules    2.5 Adult Fat Body    4.6 Adult Salivary Gland    2.1 Adult Heart    1.525 Adult VirginFemale Spermatheca    0.4 Adult InseminatedFemale Spermatheca    0.9 Adult Ovary    0.8 Adult Testis    0.9 Adult Male Accessory Gland    5.3 Adult Carcass    5.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    5.875 Larval Midgut    1.5 Larval Hindgut    1.7 Larval Malpighian Tubules    3.9 Larval Fat Body    0.7 Larval Salivary Gland    1.8 Larval Trachea    3.375 Larval Carcass    2.975 Adult Head    2.1 Adult Eye    1.7 Adult Brain    4.5 Adult Thoracic-Abdominal Ganglion    1.5 Adult Crop    5 Adult Midgut    2.3 Adult Hindgut    2.2 Adult Malpighian Tubules    2.5 Adult Fat Body    4.6 Adult Salivary Gland    2.1 Adult Heart    1.525 Adult VirginFemale Spermatheca    0.4 Adult InseminatedFemale Spermatheca    0.9 Adult Ovary    0.8 Adult Testis    0.9 Adult Male Accessory Gland    5.3 Adult Carcass    5.8 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    2.1   NA  Eye    1.7   NA  Brain    4.5   5.875  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    1.5   NA  Crop    5   1.5  Midgut    2.3   1.7  Hindgut    2.2   3.9  Malpighian Tubules    2.5   0.7  Fat Body    4.6   1.8  Salivary Gland    2.1   NA  Heart    1.525   3.375  Trachea    NA   NA  VirginFemale Spermatheca    0.4   NA  InseminatedFemale Spermatheca    0.9   NA  Ovary    0.8   NA  Testis    0.9   NA  Male Accessory Gland    5.3   2.975  Carcass    5.8 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_01
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0086899 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG32781-RB
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele cell lethal | somatic clone tlkΔ14 lethal, with Scer\GAL4pnr-MD237 tlkGD9828 lethal | embryonic stage | recessive tlkΔ14 tlkΔ40 lethal | germline clone | male limited tlkG0054 lethal | larval stage tlkG0054 lethal | larval stage | heat sensitive, with Scer\GAL4hs.2sev tlkScer\UAS.cCa lethal | larval stage | recessive tlkG0054 lethal | prepupal stage tlkG0054 lethal | pupal stage tlkG0054 lethal | pupal stage, with Scer\GAL4pnr-MD237 tlkGD3220 tlkGD15953 lethal | recessive tlkG0054 tlkPL56#2 Sterility Allele sterile | female | germline clone tlkΔ14 tlkΔ40 Other Phenotypes Allele increased cell death, with Scer\GAL4arm.PS tlkScer\UAS.cCa increased cell death, with Scer\GAL4Bx-MS1096 tlkScer\UAS.cCa increased cell death, with Scer\GAL4mat.αTub67C.T:Hsim\VP16 tlkScer\UAS.cCa increased cell death | embryonic stage tlkΔ14 mitotic cell cycle defective, with Scer\GAL4bcd3.1.T:Scer\GCN4 tlkScer\UAS.cCa mitotic cell cycle defective, with Scer\GAL4GMR.PF tlkScer\UAS.cCa mitotic cell cycle defective | embryonic stage tlkΔ14 visible, with Scer\GAL4GMR.PF tlkEP1413 Phenotype manifest in Allele embryonic/larval salivary gland, with Scer\GAL4hs.2sev tlkScer\UAS.cCa tlkdead.Scer\UAS eye, with Scer\GAL4ey.PH tlkScer\UAS.cCa tlkdead.Scer\UAS eye, with Scer\GAL4GMR.PF tlkEP1413 head, with Scer\GAL4ey.PH tlkdead.Scer\UAS larval salivary gland & nuclear chromosome, with Scer\GAL4hs.2sev tlkScer\UAS.cCa macrochaeta, with Scer\GAL4hs.2sev tlkScer\UAS.cCa
Classical Alleles ( 78 )
For All Classical Alleles Show
Allele of tlk Class Mutagen Stocks Known lesion tlkG0054 P-element activity 2 Yes tlkNP7280 2 -- tlk5-SZ-3006 1 -- tlk5-SZ-3116 1 -- tlkBG02799 1 -- tlkc00155 1 -- tlkc00749 1 -- tlkc02544 1 -- tlkCB-5803-3 1 -- tlkd04126 1 -- tlkd04235 1 -- tlkd04372 1 -- tlkd04653 1 -- tlkd09027 1 -- tlkd09371b 1 -- tlkd11280a 1 -- tlke00896 1 -- tlke01681 1 -- tlke01709 1 -- tlke02560 1 -- tlke03845 piggyBac transposase 1 -- tlkEP911 1 -- tlkEY11693 1 -- tlkEY14954 1 -- tlkEY23414 1 -- tlkf00242 1 -- tlkf01347 1 -- tlkf02966 1 -- tlkf07841 1 -- tlkf07951b 1 -- tlkG0113a 1 -- tlkG17414 P-element activity 1 -- tlkGS1207 1 Yes tlkKG02078 1 -- tlkKG06931 1 -- tlkNP0872 1 -- tlkNP1098 1 -- tlkNP2153 1 -- tlkNP3171 1 -- tlkNP3445 1 -- tlkNP3463 1 -- tlkNP4018 1 -- tlkNP5046 1 -- tlkNP5054 1 -- tlkNP6023 1 -- tlkNP7150 1 -- tlkNP7205 1 -- tlkNP7244 1 -- tlkNP7263 1 -- tlkNP7281 1 -- tlkUM-8202-3 1 -- tlkUM-8219-3 1 -- tlk27-9 P-element activity 0 Yes tlk27 0 Yes tlk5-SZ-3148 0 -- tlkCPTI002768 piggyBac transposase 0 Yes tlkCPTI003640 piggyBac transposase 0 Yes tlkd02927 0 -- tlkd04184 0 -- tlkd05898 0 -- tlkd09016 0 -- tlkd10494 0 -- tlke03278 0 -- tlke04329 0 -- tlkEP1094 0 -- tlkEP1327 0 -- tlkEP1413 P-element activity 0 -- tlkEY10218 0 -- tlkf00072 0 -- tlkf00258 0 -- tlkf00270 0 -- tlkf07789 0 -- tlkGG01057 0 -- tlkNP4076 0 -- tlkNP4095 0 -- tlkPL56#2 Delta2-3 0 -- tlkΔ14 P-element activity 0 Yes tlkΔ40 0 Yes
Alleles Carried on Transgenic Constructs ( 13 )
For All Alleles Carried on Transgenic Constructs Show
Allele of tlk Class Mutagen Stocks Known lesion tlkGD15952 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes tlkGD15953 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes tlkGD3220 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes tlkScer\UAS.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - coding region fusion 2 Yes tlkGD9828 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes tlkGL00200 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes tlkHMS00943 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes tlkJF01742 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes tlkK956E.Scer\UAS in vitro construct - site directed mutagenesis in vitro construct - regulatory fusion 1 Yes tlkKK108036 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes tlkScer\UAS.cMa in vitro construct - regulatory fusion 1 Yes tlkdead.Scer\UAS in vitro construct - site directed mutagenesis 0 Yes tlkScer\UAS.cCa in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD3220} NA P{GD9828} NA P{GD15952} NA P{GD15953} NA P{KK108036} NA P{TRiP.GL00200} NA P{TRiP.HMS00943} NA P{TRiP.JF01742} NA P{UAS-tlk.C} NA P{UAS-tlk.dead} NA P{UAS-tlk.FLAG} NA P{UAS-tlk.K956E} NA P{UAS-tlk} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}BEST:GH17330EP1408 NA P{EP}EP1200EP1200 NA P{EP}tlkEP911 NA P{EP}tlkEP1094 NA P{EP}tlkEP1327 NA P{EP}tlkEP1413 NA P{EP}tlkG17414 NA P{EPgy2}tlkEY10218 NA P{EPgy2}tlkEY11693 NA P{EPgy2}tlkEY14954 NA P{EPgy2}tlkEY23414 NA P{GSV1}tlkGS1207 NA P{Mae-UAS.6.11}tlkGG01057 NA P{XP}tlkd02927 NA P{XP}tlkd04126 NA P{XP}tlkd04184 NA P{XP}tlkd04235 NA P{XP}tlkd04372 NA P{XP}tlkd04653 NA P{XP}tlkd05898 NA P{XP}tlkd09016 NA P{XP}tlkd09027 NA P{XP}tlkd09371b NA P{XP}tlkd10494 NA P{XP}tlkd11280a NA miscellaneous insertions P{GT1}BG02107 NA P{GT1}tlkBG02799 NA P{GT1}VhaAC39-1BG01910 NA P{RS3}tlkCB-5803-3 NA P{RS3}tlkUM-8202-3 NA P{RS3}tlkUM-8219-3 NA P{RS5}tlk5-SZ-3006 NA P{RS5}tlk5-SZ-3116 NA P{RS5}tlk5-SZ-3148 NA P{SUPor-P}tlkKG02078 NA P{SUPor-P}tlkKG06931 NA PBac{768.FSVS-0}tlkCPTI003640 NA PBac{CPTI-un}tlkCPTI002768 NA PBac{PB}tlkc00155 NA PBac{PB}tlkc00749 NA PBac{PB}tlkc02544 NA PBac{RB}tlke00896 NA PBac{RB}tlke01681 NA PBac{RB}tlke01709 NA PBac{RB}tlke02560 NA PBac{RB}tlke03278 NA PBac{RB}tlke03845 NA PBac{RB}tlke04329 NA PBac{WH}tlkf00072 NA PBac{WH}tlkf00242 NA PBac{WH}tlkf00258 NA PBac{WH}tlkf00270 NA PBac{WH}tlkf01347 NA PBac{WH}tlkf02966 NA PBac{WH}tlkf07789 NA PBac{WH}tlkf07841 NA PBac{WH}tlkf07951b NA insertion of enhancer trap binary system P{GawB}tlkNP0872 No P{GawB}tlkNP1098 No P{GawB}tlkNP2153 No P{GawB}tlkNP3171 No P{GawB}tlkNP3445 No P{GawB}tlkNP3463 No P{GawB}tlkNP4018 No P{GawB}tlkNP4076 No P{GawB}tlkNP4095 No P{GawB}tlkNP5046 No P{GawB}tlkNP5054 No P{GawB}tlkNP6023 No P{GawB}tlkNP7150 No P{GawB}tlkNP7205 No P{GawB}tlkNP7244 No P{GawB}tlkNP7263 No P{GawB}tlkNP7280 No P{GawB}tlkNP7281 No insertion of enhancer trap P{lacW}l(1)G0167G0167 No P{lacW}l(1)G0299G0299 No P{lacW}l(1)G0343G0343 No P{lacW}tlk27 No P{lacW}tlkG0054 No P{lacW}tlkG0113a No P{lacW}tlkPL56#2 No
Gene Ontology: Function, Process & Cellular Component ( 15 unique terms )
Terms Based on Experimental Evidence ( 13 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with asf1 (Carrera et al., 2003) protein kinase activity inferred from direct assay (Carrera et al., 2003)
Biological Process
CV term Evidence References
antimicrobial humoral response inferred from mutant phenotype (Kleino et al., 2005) cell adhesion inferred from mutant phenotype (Kiger et al., 2003) chromatin organization inferred from genetic interaction with asf1 (Carrera et al., 2003) chromosome segregation inferred from genetic interaction with mars (Li et al., 2009) histone phosphorylation inferred from mutant phenotype (Carrera et al., 2003) mitotic cell cycle, embryonic inferred from mutant phenotype (Carrera et al., 2003) protein autophosphorylation inferred from direct assay (Carrera et al., 2003) protein phosphorylation inferred from direct assay (Carrera et al., 2003) regulation of cell cycle inferred from mutant phenotype (Carrera et al., 2003) regulation of cell shape inferred from mutant phenotype (Kiger et al., 2003)
Cellular Component
CV term Evidence References
nucleus inferred from direct assay (Carrera et al., 2003)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR017441 (FlyBase Curators et al., 2004-) protein kinase activity non-traceable author statement (Morrison et al., 2000) protein serine/threonine kinase activity inferred from sequence or structural similarity with EMBL:AF045252 (FlyBase, 1992-) inferred from sequence or structural similarity with EMBL:AF045253 (FlyBase Genome Annotators, 2004) inferred from sequence or structural similarity with human TLK1 AND inferred from sequence or structural similarity with human TLK2 AND inferred from sequence or structural similarity with TAIR:gene:1944622 (Carrera et al., 2003)
Biological Process
CV term Evidence References
protein phosphorylation inferred from sequence or structural similarity with EMBL:AF045253 (FlyBase Genome Annotators, 2004) non-traceable author statement (Morrison et al., 2000)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions tlk allele Gene References CG12462EP1413 btl (Zhu et al., 2005) Cdc42 (Zhu et al., 2005) pnr (Pena-Rangel et al., 2002) Pvr (Zhu et al., 2005) Rac1 (Zhu et al., 2005) tlkdead.Scer\UAS asf1 (Carrera et al., 2003) tlkScer\UAS.cCa asf1 (Carrera et al., 2003) unspecified H (Mueller et al., 2005) pbl (Gregory et al., 2007)
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0086899 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 318206
Orthologs
Genome-wide drosophilid orthologs
Curated drosophilid orthologs	Dpse\GA11648   Dpse\GA17134   Dsim\GD16650
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL014329-PA Aedes aegypti (mosquito) AGAP000043-PA Anopheles gambiae (mosquito) XP_624065.2 Apis mellifera (honey bee) XP_001947282.1 Acyrthosiphon pisum (pea aphid) At5g20930.1 Arabidopsis thaliana (wall cress) 71623 Branchiostoma floridae (lancelet) BGIBMGA006552-PA Bombyx mori (silk worm) CN11474 Caenorhabditis brenneri (small nematode) CBP26520 Caenorhabditis briggsae (nematode) CE36354 Caenorhabditis elegans (worm) JA08474 Caenorhabditis japonica (nematode) cgd8_1230 Cryptosporidium parvum (protozoan parasite) CPIJ009012 Culex pipens (mosquito) 178953 Chlamidomonas reinhardtii (green alga) RP38426 Caenorhabditis remanei (nematode) 174294 Capitella spl (polychaete worm) 302378 Daphnia pulex (daphnia) LmjF31.2860 Leishmania major (virus) ENSMMUP00000002885 Macaca mulatta (rhesus monkey) ENSMUSP00000102554 Mus musculus (house mouse) XP_001601609.1 Nasonia vitripennis (wasp) LOC_Os03g53880.1 Oryza sativa (asian rice) PF11_0242 Plasmodium falciparum (protozoal parasite) PHUM003306-PA Pediculus humanus (human body louse) PP15295 Pristionchus pacificus (nematode) PVX_099725 Plasmodium vivax (protozoal parasite) TA08840 Theileria annulata (apicomplexan parasite) XP_967371.2 Tribolium castaneum (red flour beetle) TP04_0518 Theileria parva (parasitic protozoan) 3830.m00511 Tetrahymena thermophila (ciliate protozoa) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV10617 LH22948 ISCW000436 FBgn0233844 FBgn0212228 FBgn0206477 FBgn0068687 FBgn0167241 JGI_V11_302378 FBgn0247156 FBgn0150605 FBgn0138590 FBgn0131671 FBgn0110878 FBgn0096167 CPIJ009012 BGIBMGA006552 ACYPI002689 GB15719 AGAP000043 AAEL014329 PB26073 PHUM466780 GLEAN_08538
Stocks & Reagents
Stocks Listed in FlyBase ( 69 )
Harvard	- P{XP}tlkd04126 - P{XP}tlkd04235 - P{XP}tlkd04372 - P{XP}tlkd04653 - P{XP}tlkd09027 - PBac{PB}tlkc00155 - PBac{PB}tlkc00749 - PBac{PB}tlkc02544 - PBac{RB}tlke00896 - PBac{RB}tlke01681 - PBac{RB}tlke01709
VDRC	v105732 P{KK108036}VIE-260B
	More stocks available...
Genomic Clones ( 3 )
	BACR19D19 BACR32E02 CH221-14P20 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 134 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	AT12465 GH07910 LD09833 LD14750 LD15160 LD40879 RE19210
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT07411 GM03476 GM19884 GM29772 HL02134 HL03509 LD01024 LD01971 LD03727 LD04615 LD07890 LD09768 LD10494 LD13226 LD15329 LD15394 LD16185 LD16882 LD18912 LD19494 LD19947 LD20029 LD28322 LD29673 LD35294 LD37602 LD38852 LD40968 LD41737 LD47166 LD47357 RE05110 RE63773 RH09985 RH10596 RH19913 RH40313 RH46508 RH51915 SD02751 SD06371 SD22806
Other clones	2214 11616 17250 17427 19412 19476 30592 37108 40082 58582 78561 102217 107408 108493 117283 122707 148287 153099 161972 192844 214339.54 214371.63 527819 569589 CK01.21C.A6 CK01.40A.G12 CK01.54D.E10 CK01.66D.E2 CK01.76D.D2 CK01.76D.F2 CK01.99D.E6 CK01.109C.C8 CK01.111A.H10 CK01.114C.G6 CK01.168D.G10 CK01643 Dmel_dig_007737_0648_3567 EC053037 EK044351 EK054049 EK060045 EK060053 EK084551 EK098944 EK103757 EK112337 EK123452 EK129724 EK132427 EK139220 EK139934 EK147859 EK153424 EK153446 EK160202 EK166412 EK169504 EK173331 EK173450 EK179402 EK180956 EK208141 EK233320 EK234205 EK242724 EK245111 EK253839 EK256443 EK262206 EK266334 EK290311 EK295851 EK298538 EK308959 EN08013 EN15214 EP01638 EP19733 FGA005H10 FGM221G11 FGM238B06 GH10307 GM08281 LD12312 LD41810
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0086899 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 31333 45092 318205 318206 2768876
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: CG12462 EP1413 (Mueller et al., 2005) Source for merge of: CG32782 CG33219 (FlyBase Genome Annotators, 2004-2005) Source for merge of: tlk anon-WO03040301.114 anon-WO03040301.116 (FlyBase, 1996-) Source for merge of: tlk CG12462 (FlyBase Genome Annotators, 2007) Source for merge of: tlk CG32781 (FlyBase Genome Annotators, 2007) Source for merge of: tlk CG32782 (FlyBase, 1996-)
Additional comments	Annotations CG32782 and CG32781 merged as CG34412 in release 5.2 of the genome annotation. (FlyBase Genome Annotators, 2007) Sequences corresponding to annotation CG12462 (which was eliminated in release 5.1 of the genome annotation) have been reinstated as part of the CG34412 annotation (which corresponds to tlk) in release 5.2 of the genome annotation. (FlyBase Genome Annotators, 2007) "l(1)G0299" may affect "CG2829". "l(1)G0167" may affect "CG2829". "l(1)G0343" may affect "CG2829". "l(1)G0054" may affect "CG2829". (Peter et al., 2002) Source for merge of tlk anon-WO03040301.114 anon-WO03040301.116 was sequence comparison (date:051113). (FlyBase, 1996-) Source for merge of tlk CG32782 was sequence comparison (date:050324). (FlyBase, 1996-)
Other Comments
	RNAi screen using dsRNA made from templates generated with primers directed against this gene causes decreased γ-tubulin staining at the spindle pole and misalignment of chromosomes along the spindle when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA. (Goshima et al., 2007) Annotation CG12462 eliminated in release 5.1 of the genome annotation. (FlyBase Genome Annotators, 2006) RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a greater than three-fold increase in AttA activity in response to heat-killed E.coli after ecdysone treatment in S2 cells. (Kleino et al., 2005) When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an increase in the proportion of S phase cells, G2/M+, an increase in the proportion of G2/M phase cells, a decrease in cytokinetic index and a decrease in the mitotic index are seen. (Bettencourt-Dias et al., 2004) New annotation (CG33219) in release 3.2 of the genome annotation. (FlyBase Genome Annotators, 2004) The release 4 CG32782 annotation contains sequences corresponding to release 2 annotation CG2829. The release 3 version of CG32782 was incomplete and was missing most of the sequence corresponding to release 2 annotation CG2829 (possibly due to an assembly error), but this has been fixed in release 4 and the release 4 CG32782 annotation now includes all of the release 2 CG2829 sequence. 'CG33219' does not appear in Release 4. The region it was located in in Release 3.2 is now a large TE. 'CG33219' is homologous to annotation CG32782; presumably that is where this little bit of mislaid 'CG33219' sequence originated. (FlyBase Genome Annotators, 2004-2005) The tlk-asf1 pathway is important for both DNA replication and chromatin assembly. tlk may coordinate cell cycle progression through the regulation of chromatin dynamics. (Carrera et al., 2003) RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R+ cells: cells become round and detached. (Kiger et al., 2003) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) New annotation (CG32782) in release 3 of the genome annotation. Annotation CG2829 was eliminated in release 3 of the genome annotation, October 2002. (FlyBase Genome Annotators, 2002-2003) New annotation (CG32781) in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_206620 NM_001103403 NM_001144680 NM_001144681 NM_001144682 NM_001144683 NM_001169184 RefSeq (Proteins) • NP_996343 NP_001096873 NP_001138152 NP_001138153 NP_001138154 NP_001138155 NP_001162655 DDBJ / EMBL / GenBank • AAN09103 AAN09104 AAS72337 ACL82885 ACL82886 ACL82887 ACL82888 ACZ95192 AQ025450 Entrez Gene - A searchable database of RefSeq genes. • 318206 UniProtKB/TrEMBL • B7Z120 B7Z126 Q1RL19 Q8IRT8
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, ATP binding site (IPR017441) Serine/threonine-protein kinase-like domain (IPR017442)
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0086899 DRSC - Results from RNAi screens. • FBgn0086899 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0086899 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG32781-RB FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0086899 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 31333 45092 318205 318206 2768876 InParanoid A subset of ortholog calls from InParanoid. • AAEL014329-PA Aedes aegypti (mosquito) AGAP000043-PA Anopheles gambiae (mosquito) XP_624065.2 Apis mellifera (honey bee) XP_001947282.1 Acyrthosiphon pisum (pea aphid) At5g20930.1 Arabidopsis thaliana (wall cress) 71623 Branchiostoma floridae (lancelet) BGIBMGA006552-PA Bombyx mori (silk worm) CN11474 Caenorhabditis brenneri (small nematode) CBP26520 Caenorhabditis briggsae (nematode) CE36354 Caenorhabditis elegans (worm) JA08474 Caenorhabditis japonica (nematode) cgd8_1230 Cryptosporidium parvum (protozoan parasite) CPIJ009012 Culex pipens (mosquito) 178953 Chlamidomonas reinhardtii (green alga) RP38426 Caenorhabditis remanei (nematode) 174294 Capitella spl (polychaete worm) 302378 Daphnia pulex (daphnia) LmjF31.2860 Leishmania major (virus) ENSMMUP00000002885 Macaca mulatta (rhesus monkey) ENSMUSP00000102554 Mus musculus (house mouse) XP_001601609.1 Nasonia vitripennis (wasp) LOC_Os03g53880.1 Oryza sativa (asian rice) PF11_0242 Plasmodium falciparum (protozoal parasite) PHUM003306-PA Pediculus humanus (human body louse) PP15295 Pristionchus pacificus (nematode) PVX_099725 Plasmodium vivax (protozoal parasite) TA08840 Theileria annulata (apicomplexan parasite) XP_967371.2 Tribolium castaneum (red flour beetle) TP04_0518 Theileria parva (parasitic protozoan) 3830.m00511 Tetrahymena thermophila (ciliate protozoa) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 318206 modMine - Data generated by the modENCODE project. • FBgn0086899 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV10617 LH22948 ISCW000436 FBgn0233844 FBgn0212228 FBgn0206477 FBgn0068687 FBgn0167241 JGI_V11_302378 FBgn0247156 FBgn0150605 FBgn0138590 FBgn0131671 FBgn0110878 FBgn0096167 CPIJ009012 BGIBMGA006552 ACYPI002689 GB15719 AGAP000043 AAEL014329 PB26073 PHUM466780 GLEAN_08538
Synonyms & Secondary IDs ( 18 )
Reported As
Symbol Synonym	anon-WO03040301.114   anon-WO03040301.116   BcDNA:GH07910 (Peter et al., 2002, ) CG2829 (Bettencourt-Dias et al., 2004, Zhu et al., 2005, Morrison et al., 2000, Whitfield, 2000.5.10, Kiger et al., 2003, Kiger et al., 2003, Brody et al., 2002, ) CG12462 (Gilfillan et al., 2006, Gregory et al., 2007) CG32781 (Gilfillan et al., 2006, ) CG32782 (Kleino et al., 2005, Blanco et al., 2010, Goshima et al., 2007, Hogan and Bettencourt, 2009) CG33219 (FlyBase Genome Annotators, 2004-2005) CG34412 (FlyBase Genome Annotators, 2007, Keleman et al., 2009.8.5, Ni et al., 2008, Ni et al., 2010.12.1, Ni et al., 2011.7.19) dtlk (Chou and Liaw, 2006) EP1413 (Zhu et al., 2005, ) EP(X)1413 (Pena-Rangel et al., 2002) l(1)G0054 (Peter et al., 2002, Schaefer et al., 1999.6.15, Schaefer et al., 1999.3.29, ) tlk (Gilfillan et al., 2006, Gan et al., 2010, Stofanko et al., 2008, Manak et al., 2006, Hogan and Bettencourt, 2009, Li et al., 2009, Merdes, 2010.2.24, Zhou et al., 2008, Merdes, 2010.8.5, Mourikis et al., 2010, Reis et al., 2010) Tlk (Goshima et al., 2007) TLK (Pilyugin et al., 2009, Moshkin et al., 2009)
Name Synonym	CG12462   CG32781   Tousled-like kinase (Carrera et al., 2003, Merdes, 2010.2.24, Hogan and Bettencourt, 2009, Merdes, 2010.8.5) tousled-like kinase (Stofanko et al., 2008, Li et al., 2009)
Secondary FlyBase IDs
	FBan0032782 FBgn0026698 FBgn0028489 FBgn0029675 FBgn0052781 FBgn0052782 FBgn0053219 FBgn0062688 FBgn0066855 FBgn0066856 FBgn0085441 FBgn0086709
References ( 60 )
Generate a list of	All references relating to this gene ( 60 )
List References by type	Research paper  ( 24 ) Supplementary material  ( 7 ) Review  ( 1 ) Personal communication to FlyBase  ( 13 ) Abstract  ( 1 ) FlyBase analysis  ( 8 ) Computer file  ( 3 ) Automatic genome annotation  ( 1 ) Patent  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 4 )
	Blanco et al., 2010, BMC Dev. Biol. 10: 94 Gene expression following induction of regeneration in Drosophila wing imaginal discs. Expression profile of regenerating wing discs. [FBrf0211812] Gan et al., 2010, Cell Res. 20(7): 763--783 Dynamic regulation of alternative splicing and chromatin structure in Drosophila gonads revealed by RNA-seq. [FBrf0211191] Mourikis et al., 2010, BMC Dev. Biol. 10: 107 Modifiers of notch transcriptional activity identified by genome-wide RNAi. [FBrf0212287] Reis et al., 2010, PLoS Genet. 6(11): e1001206 A buoyancy-based screen of Drosophila larvae for fat-storage mutants reveals a role for sir2 in coupling fat storage to nutrient availability. [FBrf0212335] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...