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General Information
Symbol
Dmel\sls
Species
D. melanogaster
Name
sallimus
Annotation Symbol
CG1915
Feature Type
FlyBase ID
FBgn0086906
Gene Model Status
Stock Availability
Gene Snapshot
sallimus (sls) encodes a large modular protein present in muscles. It links myosin filaments to the Z-disc. [Date last reviewed: 2019-03-14]
Also Known As

D-Titin, kettin, Titin, Ket, l(3)dre8

Key Links
Genomic Location
Cytogenetic map
Sequence location
3L:2,039,681..2,115,611 [-]
Recombination map

3-1.5

RefSeq locus
NT_037436 REGION:2039681..2115611
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (18 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
inferred from direct assay
(assigned by UniProt )
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
(assigned by UniProt )
Terms Based on Predictions or Assertions (0 terms)
Biological Process (10 terms)
Terms Based on Experimental Evidence (10 terms)
CV Term
Evidence
References
inferred from mutant phenotype
(assigned by UniProt )
inferred from expression pattern
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:Mlp84B; FB:FBgn0014863
inferred from mutant phenotype
(assigned by UniProt )
inferred from direct assay
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
(assigned by UniProt )
inferred from expression pattern
(assigned by BHF-UCL )
inferred from expression pattern
(assigned by BHF-UCL )
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (5 terms)
Terms Based on Experimental Evidence (5 terms)
CV Term
Evidence
References
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
inferred from direct assay
inferred from direct assay
(assigned by UniProt )
inferred from mutant phenotype
(assigned by UniProt )
inferred from high throughput direct assay
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
(assigned by BHF-UCL )
Terms Based on Predictions or Assertions (0 terms)
Protein Family (UniProt)
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. (Q9I7U4)
Summaries
Protein Function (UniProtKB)
Key component in the assembly and functioning of adult and embryonic striated muscles and muscle tendons. By providing connections at the level of individual microfilaments, it contributes to the fine balance of forces between the two halves of the sarcomere. The size and extensibility of the cross-links are the main determinants of sarcomere extensibility properties of muscle. In non-muscle cells, seems to play a role in chromosome condensation and chromosome segregation during mitosis. Might link the lamina network to chromatin or nuclear actin, or both during interphase.
(UniProt, Q9I7U4)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
l(3)62Ca
Hemizygotes for seventeen of nineteen alleles die during the first larval instar; four of these show some escape into the second instar; two alleles cause lethality in the pupal-adult stage.
sls: sallimus (J.A. Kennison)
Dominant suppressor of the extra-sex-combs phenotype of heterozygous Pc alleles. Also suppresses the dominant extra-sex-combs phenotype of Pcl alleles.
Gene Model and Products
Number of Transcripts
13
Number of Unique Polypeptides
13

Please see the GBrowse view of Dmel\sls or the JBrowse view of Dmel\sls for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.40

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.45

Gene model reviewed during 5.55

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0072848
15263
4796
FBtr0309042
17268
4811
FBtr0332674
56489
18468
FBtr0332675
56405
18440
FBtr0332676
55436
18117
FBtr0332677
47528
15481
FBtr0332678
55136
18017
FBtr0332679
48944
15953
FBtr0332680
49523
16146
FBtr0332681
47519
15478
FBtr0332682
55178
18031
FBtr0332683
32960
10625
FBtr0332684
46550
15155
Additional Transcript Data and Comments
Reported size (kB)

18.0, 14.5 (northern blot)

11.5 (northern blot)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0072727
540.7
4796
5.89
FBpp0301063
542.5
4811
5.88
FBpp0304920
2102.8
18468
4.66
FBpp0304921
2099.5
18440
4.66
FBpp0304922
2063.4
18117
4.66
FBpp0304923
1759.9
15481
4.65
FBpp0304924
2051.1
18017
4.66
FBpp0304925
1810.7
15953
4.70
FBpp0304926
1844.1
16146
4.70
FBpp0304927
1760.3
15478
4.64
FBpp0304928
2053.6
18031
4.66
FBpp0304929
1207.3
10625
4.81
FBpp0304930
1724.3
15155
4.64
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)

4796 (aa); 540 (kD predicted)

Comments
External Data
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\sls using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
expression microarray
Stage
Tissue/Position (including subcellular localization)
Reference
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
RT-PCR
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

The 18kb Ket transcript is a minor species observed on northern blots.

Ket transcripts are first expressed at stage 11 in the mesoderm. They are expressed in most if not all muscles until the third instar larval stage. At stage 14, they are expressed in nearly all somatic, visceral, and pharyngeal muscles. Later they are expressed in muscle attachment sites.

Ket transcripts are first detected in early stage 11 embryos. Expression is observed mainly in the hindgut and posterior midgut invagination. By late stage 11, expression is observed throughout the mesoderm and can be seen in the progenitors to the pharyngeal muscles and salivary glands. By stage 12,13, the mesodermal pattern has resolved into a parasegmental pattern. By stage 13,14, expression is present in both somatic and visceral mesoderm.

Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

sls protein can be detected in scattered aggregates dispersed in the cytoplasm of muscle cell precursors as early at 30 hrs APF. By 48 hours sls, along with other myofibrillar proteins, have organized into Z-bands.

The protein is enriched at the membrane of fusion competent cells and primarily cytoplasmic in founder cells in the developing muscle system. In myotubes sls protein islocated near the points of contact with myoblasts.

Ket protein localizes to the nucleus during interphase and to the chromosomes during mitosis.

Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
inferred from direct assay
inferred from direct assay
(assigned by UniProt )
inferred from mutant phenotype
(assigned by UniProt )
inferred from high throughput direct assay
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
(assigned by BHF-UCL )
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\sls in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 13-16
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 69 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of sls
Transgenic constructs containing regulatory region of sls
Deletions and Duplications ( 157 )
Disrupted in
Not disrupted in
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
indirect flight muscle & myofibril
indirect flight muscle & striated muscle thick filament
indirect flight muscle & Z disc
mitosis & nuclear chromosome
mitosis & nuclear chromosome (with sls110ry-)
mitosis & nuclear chromosome (with slse4)
mitosis & nuclear chromosome (with slsg65)
mitosis & nuclear chromosome (with slsj1D7)
muscle fiber & nucleus & embryo
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (38)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
3 of 15
Yes
Yes
3 of 15
Yes
No
2 of 15
No
No
2 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1  
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
3  
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (37)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
4 of 15
Yes
Yes
3 of 15
No
Yes
3 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Rattus norvegicus (Norway rat) (31)
3 of 13
Yes
Yes
2 of 13
No
Yes
2 of 13
No
Yes
2 of 13
No
No
1 of 13
No
No
1 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
Yes
1 of 13
No
No
1 of 13
No
No
1 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
No
1 of 13
No
No
1 of 13
No
Yes
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
Yes
1 of 13
No
No
1 of 13
No
No
1 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (25)
2 of 12
Yes
Yes
1 of 12
No
No
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
Yes
1 of 12
No
No
1 of 12
No
Yes
1 of 12
No
No
1 of 12
No
No
Danio rerio (Zebrafish) (65)
3 of 15
Yes
Yes
2 of 15
No
No
2 of 15
No
Yes
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (30)
10 of 15
Yes
Yes
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (2)
1 of 15
Yes
No
1 of 15
Yes
No
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190001 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915000C )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0007 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Pediculus humanus
Human body louse
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0006 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G000F )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (23)
2 of 10
2 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 3 )
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser

    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    RNA-RNA
    Physical Interaction
    Assay
    References
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    External Data
    Linkouts
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    3L
    Recombination map

    3-1.5

    Cytogenetic map
    Sequence location
    3L:2,039,681..2,115,611 [-]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    62C2-62C4
    Limits computationally determined from genome sequence between P{PZ}dlt04276 and P{PZ}slsrL182
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    62C-62C
    (determined by in situ hybridisation)
    62C1-62C2
    (determined by in situ hybridisation) 62C (determined by in situ hybridisation)
    62C1-62C1
    62C1-62C3
    (determined by in situ hybridisation)
    62C1-62C2
    (determined by in situ hybridisation) 62C3--4 (determined by in situ hybridisation)
    62C1-62C2
    (determined by in situ hybridisation)
    68B8-68F2
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (19)
    Genomic Clones (32)
    cDNA Clones (272)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
    Commercially Available Antibodies
     
    Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
    Other Information
    Relationship to Other Genes
    Source for database identify of

    Source for identity of: Titin CG1915

    Source for database merge of

    Source for merge of: sls l(3)S002001

    Source for merge of: Ket Titin

    Source for merge of: Ket l(3)rL182 l(3)62Ca

    Source for merge of: sls Ket

    Source for merge of: sls CG18857

    Source for merge of: sls CG18242

    Additional comments

    FlyBase curator comment: Correspondence with author Sparrow confirmed that 'Salimus' is identical to 'sallimus' (CG1915).

    Annotations CG1915, CG18242 and CG18857 merged as CG1915 in release 3 of the genome annotation.

    Other Comments

    tn, Actn, sls and Mlp84B act in concert to maintain the integrity and stability of Z-discs, thereby ensuring myofibril stability and optimal muscle function.

    ChEST reveals this is a target of Mef2.

    dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

    sls may be involved in the morphogenesis of leg imaginal discs, and it is necessary for condensation and separation of sister chromatids during mitosis.

    sls along with bt protein may determine most of the high passive stiffness of the indirect flight muscle that is necessary for stretch activation.

    sls is essential for the formation and maintenance of normal sarcomere structure of muscles and muscle tendons.

    Mutations in sls result in chromosome undercondensation, chromosome breakage, loss of diploidy and premature sister chromatid separation. Mutants also have defects in myoblast fusion and muscle organisation.

    sls has a role in myoblast fusion during myogenesis and later serves to organise myofilaments into the highly ordered arrays underlying skeletal muscle striation.

    maps to clone: DS07136

    Identification: Via an autoimmune serum from a scleroderma patient. During embryogenesis, sls RNA and protein accumulate in all the somatic and visceral muscles. Antisera made against two different domains of sls localize this portion of the protein to the Z-discs of both larval visceral and adult thoracic muscles by confocal microscopy.

    sls has been cloned and its expression pattern has been analysed. Isolated from a genomic library using human autoimmune scleroderma serum that identifies a chromosomal protein in human cells and Drosophila embryos.

    Identified in a screen for modifiers of the Dfd13/Dfd3 mutant phenotype. Shows no interaction with the Pc mutant phenotype.

    sls encodes a mitotic chromosomal epitope and is expressed in the somatic musculature during late embryogenesis.

    sls encodes a mitotic chromosomal epitope.

    Isolated from a screen of a cDNA expression library with a human CREST serum.

    sls is required for the function of other homeotic genes.

    Sections of the Scr regulatory region may be important for regulation of Scr by Polycomb- and trithorax-group genes.

    Mutations of sls interact with Dfd to reduce the viability of the Dfd3/Dfd13 combination.

    Identified as the gene encoding the epitope for a monoclonal antibody, MAC155, that recognises the Z-discs of Drosophila and Lethocerus. The encoded protein may act as scaffolding in the Z-disc.

    Recessive lethal mutations in this complementation group were identified by failure to complement the recessive lethality of Df(3L)R-R2.

    sls is one of the 18 loci identified in a screen for dominant modifiers of Pc and/or Antp phenotypes.

    Hemizygotes for seventeen of nineteen alleles die during the first larval instar; four of these show some escape into the second instar; two alleles cause lethality in the pupal-adult stage.

    Origin and Etymology
    Discoverer

    Kennison, 1984.

    Etymology

    'sallimus' is Finnish for 'fate'.

    Named 'Kettin' after the German word 'Kette', meaning 'chain'.

    Identification
    External Crossreferences and Linkouts ( 159 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    KEGG Genes - Molecular building blocks of life in the genomic space.
    modMine - A data warehouse for the modENCODE project
    SignaLink - A signaling pathway resource with multi-layered regulatory networks.
    Linkouts
    ApoDroso - Functional genomic database for photoreceptor development, survival and function
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
    FlyMine - An integrated database for Drosophila genomics
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Synonyms and Secondary IDs (41)
    Reported As
    Symbol Synonym
    CG18245
    CG18857
    MCP
    anon-CREST