FB2025_05 , released December 11, 2025
Gene: Dmel\egg
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General Information
Symbol
Dmel\egg
Species
D. melanogaster
Name
eggless
Annotation Symbol
CG12196
Feature Type
protein_coding_gene
FlyBase ID
FBgn0086908
Gene Model Status
Current
Stock Availability
21 publicly available
Enzyme Name (EC)
[histone H3]-N(6),N(6)-dimethyl-lysine(9) N-methyltransferase (2.1.1.366)
Gene Summary
eggless (egg) encodes a histone methyltransferase involved in epigenetic regulation of gene expression. It contributes to stem cell asymmetric division, oogenesis and wing disc development. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)
All Summaries
Also Known As

dSETDB1, SetDB1, DmSetdb1, dEset

Key Links
Genomic Location
Cytogenetic map
60E1-60E1
Sequence location
Recombination map
2-107
RefSeq locus
NT_033778 REGION:24775534..24779901
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (18 terms)
Molecular Function (6 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
enables histone H3K9 methyltransferase activity
inferred from mutant phenotype
(Sienski et al., 2015, Clough et al., 2007, Seum et al., 2007)
inferred from direct assay
(Tzeng et al., 2007)
enables histone H3K9me2 methyltransferase activity
inferred from direct assay
(Clough et al., 2007)
enables protein binding
inferred from physical interaction with FLYBASE:Pof; FB:FBgn0035047
(Tzeng et al., 2007)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
enables DNA binding
inferred from electronic annotation with InterPro:IPR001739, InterPro:IPR016177
(InterPro Project Members, 2004-)
enables histone H3K9 methyltransferase activity
inferred from biological aspect of ancestor with PANTHER:PTN002339393
(GO Reference Genome Project, 2011-)
enables histone methyltransferase activity
inferred from electronic annotation with InterPro:IPR007728
(InterPro Project Members, 2004-)
enables zinc ion binding
inferred from electronic annotation with InterPro:IPR007728
(InterPro Project Members, 2004-)
Biological Process (8 terms)
Terms Based on Experimental Evidence (7 terms)
CV Term
Evidence
References
involved_in epigenetic regulation of gene expression
inferred from mutant phenotype
(Tzeng et al., 2007)
involved_in female germ-line stem cell asymmetric division
inferred from direct assay
(Yoon et al., 2008)
involved_in heterochromatin formation
inferred from mutant phenotype
(Eastwood et al., 2021, Hines et al., 2009, Seum et al., 2007)
involved_in heterochromatin organization
inferred from mutant phenotype
(Sienski et al., 2015)
involved_in oogenesis
inferred from mutant phenotype
(Clough et al., 2014, Clough et al., 2007)
inferred from direct assay
(Yoon et al., 2008)
involved_in transposable element silencing by heterochromatin formation
inferred from genetic interaction with FLYBASE:Panx; FB:FBgn0034617
(Sienski et al., 2015)
involved_in transposable element silencing by piRNA-mediated heterochromatin formation
inferred from mutant phenotype
(Sienski et al., 2015, Yu et al., 2015)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
involved_in heterochromatin organization
inferred from biological aspect of ancestor with PANTHER:PTN002339393
(GO Reference Genome Project, 2011-)
involved_in negative regulation of gene expression
inferred from biological aspect of ancestor with PANTHER:PTN002339393
(GO Reference Genome Project, 2011-)
Cellular Component (4 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
colocalizes_with chromocenter
inferred from direct assay
(Seum et al., 2007)
located_in cytoplasm
inferred from direct assay
(Clough et al., 2007)
located_in nucleus
inferred from direct assay
(Koch et al., 2009, Clough et al., 2007)
located_in polytene chromosome
inferred from direct assay
(Seum et al., 2007, Stabell et al., 2006)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
is_active_in nucleus
inferred from biological aspect of ancestor with PANTHER:PTN002339393
(GO Reference Genome Project, 2011-)
located_in nucleus
inferred from electronic annotation with InterPro:IPR007728
(InterPro Project Members, 2004-)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily. (Q32KD2)
Protein Signatures (InterPro)
Catalytic Activity (EC/Rhea)
histone H3K9me2 methyltransferase activity
N(6),N(6)-dimethyl-L-lysyl(9)-[histone H3] + S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(9)-[histone H3] + S-adenosyl-L- homocysteine + H(+) (2.1.1.366)
RHEA 60288:
Summaries
Gene Snapshot
eggless (egg) encodes a histone methyltransferase involved in epigenetic regulation of gene expression. It contributes to stem cell asymmetric division, oogenesis and wing disc development. [Date last reviewed: 2019-03-07]
Gene Group (FlyBase)
SET DOMAIN LYSINE METHYLTRANSFERASES -
SET domain lysine methyltransferases (KMTs) catalyze the addition of methyl groups to lysine residues. SET domain KMTs possess a domain that was original characterized in the D.mel KMTs Su(var)3-9, E(z) and trx. (Adapted from FBrf0216343).
Protein Function (UniProtKB)
Histone methyltransferase that specifically trimethylates 'Lys-10' of histone H3 (H3K9me3) in ovary (PubMed:17164421). H3K9me3 represents a specific tag for epigenetic transcriptional repression by recruiting Su(var)205/HP1 to methylated histones (PubMed:17164421). Plays a central role during oogenesis (PubMed:17164421).
(UniProt, Q32KD2)
Summary (Interactive Fly)

SET domain hydroxymethyltransferase required for oogenesis - trimethylates histone H3 at its K9 residue - involved in gene silencing

Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\egg for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q32KD2)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
PDB - An information portal to biological macromolecular structures
Comments on Gene Model

Gene model reviewed during 5.47

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
egg-RA
FBtr0112777
NM_138122
3937
1262
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
egg-PA
FBpp0111689
141.9
1262
5.16
Q32KD2
NP_611966
AAF47268
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Domain

In the pre-SET domain, Cys residues bind 3 zinc ions that are arranged in a triangular cluster; some of these Cys residues contribute to the binding of two zinc ions within the cluster.

(UniProt, Q32KD2)
Crossreferences
InterPro - A database of protein families, domains and functional sites
PDB - An information portal to biological macromolecular structures
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\egg using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.24

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
anterior endoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
dorsal ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
mesoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
organism | faint | ubiquitous
(Fisher et al., 2012)
organism | ubiquitous
• cytoplasm | basal

Comment: apically cleared

(Fisher et al., 2012)
embryonic stage 7 -- 8
anterior endoderm anlage
(Fisher et al., 2012)
head mesoderm
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
dorsal ectoderm
(Fisher et al., 2012)
hindgut anlage
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 9 -- 10
anterior endoderm
(Fisher et al., 2012)
head mesoderm
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
amnioserosa
(Fisher et al., 2012)
antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
inclusive hindgut primordium
(Fisher et al., 2012)
organism | faint | ubiquitous
(Fisher et al., 2012)
posterior endoderm
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 11 -- 12
central brain primordium
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 13 -- 16
embryonic brain
(Fisher et al., 2012)
larval ventral nerve cord
(Fisher et al., 2012)
larval nervous system
(Fisher et al., 2012)
germline cell
(Fisher et al., 2012)
gonad
(Fisher et al., 2012)
adult stage | female
germarium
(Yoon et al., 2008)
oogenesis stage S10
egg chamber
(Yoon et al., 2008)
RT-PCR
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage -- adult stage
organism
(Yoon et al., 2008)
adult stage | female
adult abdomen
(Yoon et al., 2008)
Additional Descriptive Data

Expression levels of egg transcript oscillates continously throughout the life cycle. High expression levels are observed in the adult abdomen, with minimal expression in the head or thorax.

(Yoon et al., 2008)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage | female
germarium
(Yoon et al., 2008)
oogenesis
germarium
(Clough et al., 2007)
female germline stem cell
(Clough et al., 2007)
female germline cyst
(Clough et al., 2007)
follicle cell
(Clough et al., 2007)
nurse cell
(Clough et al., 2007)
oocyte
(Clough et al., 2007)
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage | female
adult abdomen
(Yoon et al., 2008)
ovary
(Yoon et al., 2008)
Additional Descriptive Data

egg protein is expressed strongly in germ stem cells and dividing germline cyst cells in the germarium in both nucleus and cytoplasm, but after the 16 cell germline cysts form, it accumulates preferentially in the nucleus. It is present at low levels in somatic cells in regions 2 and 3 of the germarium, including the prefollicular cells and the follicle cells of stage 1 egg chambers. egg protein levels increase in follicle cells shortly after egg chambers bud off. By mid-oogenesis, egg protein levels decrease in nurse cells, and increase in follicle cells. In the oocyte nucleus, egg protein is present throughout the nucleoplasm and also

(Clough et al., 2007)

localizes to distinct subnuclear sites associated with chromosomes.

(Clough et al., 2007)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
colocalizes_with chromocenter
inferred from direct assay
(Seum et al., 2007)
located_in cytoplasm
inferred from direct assay
(Clough et al., 2007)
located_in nucleus
inferred from direct assay
(Koch et al., 2009, Clough et al., 2007)
located_in polytene chromosome
inferred from direct assay
(Seum et al., 2007, Stabell et al., 2006)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\egg in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 28 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 35 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of egg
Transgenic constructs containing regulatory region of egg
Aberrations (Deficiencies and Duplications) ( 5 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal, with Scer\GAL4Act5C.PI
lethal (with Df(2R)Dll-MP)
lethal | male (with Df(2R)Dll-MP)
lethal | male (with Df(2R)ED4065)
lethal | recessive
lethal - all die before end of P-stage, with Scer\GAL4da.G32
lethal - all die before end of pupal stage, with Scer\GAL4c698a
lethal - all die before end of pupal stage | recessive
partially lethal, with Scer\GAL4Act5C.PU
partially lethal (with Df(2R)Dll-MP)
partially lethal - majority die
partially lethal - majority die (with Df(2R)Dll-MP)
partially lethal - majority die (with Df(2R)ED4065)
partially lethal - majority die (with Df(2R)ED4071)
partially lethal - majority die (with eggGS7132)
partially lethal - majority die (with eggnull)
partially lethal - majority die | recessive
viable
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
viable (with Df(2R)Dll-MP)
Sterility
Allele
female sterile
female sterile (with Df(2R)Dll-MP)
female sterile | germline clone | recessive
female sterile | temperature conditional (with Df(2R)Dll-MP)
fertile
sterile
Other Phenotypes
Allele
abnormal body color, with Scer\GAL4pnr-MD237
abnormal developmental rate | larval stage, with Scer\GAL4da.G32
abnormal eclosion
abnormal locomotor behavior
abnormal locomotor behavior | adult stage (with Df(2R)Dll-MP)
abnormal locomotor behavior | adult stage (with Df(2R)ED4065)
abnormal locomotor behavior | larval stage, with Scer\GAL4Act5C.PU
abnormal size | adult stage (with Df(2R)Dll-MP)
decreased cell number | germline clone | oogenesis | progressive
decreased cell number | oogenesis (with Df(2R)Dll-MP)
decreased cell number | oogenesis | progressive | somatic clone
decreased occurrence of cell division | oogenesis (with Df(2R)Dll-MP)
increased cell death (with Df(2R)Dll-MP)
increased cell death | germline clone | oogenesis
increased cell death | oogenesis, with Df(2R)Dll-MP/eggnull, Scer\GAL4NP1624
increased cell death | oogenesis (with Df(2R)Dll-MP), with Scer\GAL4NP1624, eggUAS.cTa
increased cell death | oogenesis | somatic clone
increased cell number | oogenesis (with Df(2R)Dll-MP)
increased mortality | P-stage
majority die during embryonic stage | maternal effect, with JabbaUASp.VHH(GFP4), Scer\GAL4VP16.mat.αTub67C
non-suppressor of variegation, with P{RS5}5-HA-1005
non-suppressor of variegation | dominant, with In(1)wm4
non-suppressor of variegation | dominant, with P{hsp26-pt-T}8-M76
non-suppressor of variegation | dominant, with P{hsp26-pt-T}39C-66
non-suppressor of variegation | dominant, with P{RS3}panCB-5204-3
non-suppressor of variegation | dominant, with P{RS5}5-HA-1902
non-suppressor of variegation | dominant, with P{RS5}5-HA-1992
non-suppressor of variegation | dominant, with T(2;3)V21-P{lacW}92E.x3
short lived, with Scer\GAL4Act5C.PU
short lived | male (with Df(2R)Dll-MP)
short lived | male (with Df(2R)ED4065)
some die during P-stage, with Scer\GAL4da.G32
some die during pupal stage, with Scer\GAL4c698a
some die during pupal stage | recessive
suppressor of variegation
suppressor of variegation, with P{hsp26-pt-T}118E-10
suppressor of variegation | dominant, with P{hsp26-pt-T}39C-66
suppressor of variegation | recessive
visible, with Scer\GAL4lz-gal4
visible, with Scer\GAL4pnr-MD237
visible | adult stage, with Scer\GAL4en.PU
visible | adult stage, with Scer\GAL4ey.PH
visible | adult stage (with Df(2R)Dll-MP)
visible | adult stage (with Df(2R)ED4065)
Phenotype manifest in
Allele
chromocenter, with Scer\GAL4da.G32
dorsal appendage | egg stage (with Df(2R)Dll-MP)
dorsal appendage | oogenesis (with Df(2R)Dll-MP)
egg, with Scer\GAL4nanos.PG
egg chamber (with Df(2R)Dll-MP)
egg chamber | germline clone
egg chamber | oogenesis (with Df(2R)Dll-MP)
egg chorion (with Df(2R)Dll-MP)
eye, with Scer\GAL4ey.PH
eye disc posterior to the morphogenetic furrow, with Scer\GAL4lz-gal4
female germline cyst | germline clone | oogenesis | progressive
female germline cyst | oogenesis (with Df(2R)Dll-MP)
follicle cell (with Df(2R)Dll-MP)
follicle stem cell | oogenesis | progressive | somatic clone
germarium (with Df(2R)Dll-MP)
germarium | oogenesis (with Df(2R)Dll-MP)
germline cyst (with Df(2R)Dll-MP)
germline stem cell | germline clone | oogenesis | progressive
interommatidial bristle
interommatidial bristle, with Scer\GAL4lz-gal4
mesonotum, with Scer\GAL4pnr-MD237
nurse cell (with Df(2R)Dll-MP)
nurse cell | oogenesis (with Df(2R)Dll-MP)
ommatidium, with Scer\GAL4lz-gal4
oocyte (with Df(2R)Dll-MP)
oocyte | oogenesis (with Df(2R)Dll-MP)
ovary
ovary (with Df(2R)Dll-MP)
ovary (with Df(2R)ED4065)
polytene chromosome, with Scer\GAL4da.G32
posterior wing margin, with Scer\GAL4en.PU
wing
wing, with Scer\GAL4Act5C.PU
wing, with Scer\GAL4ap-md544
wing (with Df(2R)Dll-MP)
wing (with Df(2R)ED4065)
wing (with eggGS7132)
wing (with eggnull)
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (16)
Hsap\SETDB1
12 of 14
Yes
Yes
1  
Hsap\SETDB2
6 of 14
No
Yes
Hsap\NSD1
2 of 14
No
No
Hsap\SUV39H1
2 of 14
No
No
4  
Hsap\SUV39H2
2 of 14
No
No
Hsap\EHMT1
1 of 14
No
No
1  
Hsap\EHMT2
1 of 14
No
No
Hsap\EZH1
1 of 14
No
No
Hsap\KMT2A
1 of 14
No
No
3  
Hsap\KMT2B
1 of 14
No
No
Hsap\KMT2C
1 of 14
No
No
Hsap\KMT2D
1 of 14
No
No
Hsap\NSD2
1 of 14
No
No
1  
Hsap\SETBP1
1 of 14
No
No
Hsap\SETD2
1 of 14
No
No
Hsap\SETDB2-PHF11
1 of 14
No
Yes
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (13)
Rnor\Setdb1
11 of 14
Yes
Yes
Rnor\Setdb2
6 of 14
No
Yes
Rnor\Nsd1
2 of 14
No
No
Rnor\Suv39h1l1
2 of 14
No
No
Rnor\Suv39h2
2 of 14
No
No
Rnor\Ehmt1
1 of 14
No
No
Rnor\Ehmt2
1 of 14
No
No
Rnor\Ezh1
1 of 14
No
No
Rnor\Kmt2a
1 of 14
No
No
Rnor\Kmt2b
1 of 14
No
No
Rnor\Kmt2d
1 of 14
No
Yes
Rnor\Nsd2
1 of 14
No
No
Rnor\Setd2
1 of 14
No
No
Mus musculus (laboratory mouse) (18)
Mmus\Setdb1
10 of 14
Yes
Yes
Mmus\Setdb2
5 of 14
No
Yes
Mmus\Nsd1
2 of 14
No
No
Mmus\Suv39h1
2 of 14
No
No
Mmus\Suv39h2
2 of 14
No
No
Mmus\Ehmt1
1 of 14
No
No
Mmus\Ehmt2
1 of 14
No
No
Mmus\Ezh1
1 of 14
No
No
Mmus\Gm45935
1 of 14
No
Yes
Mmus\Kmt2a
1 of 14
No
No
Mmus\Kmt2b
1 of 14
No
No
Mmus\Kmt2c
1 of 14
No
No
Mmus\Kmt2d
1 of 14
No
No
Mmus\Nsd2
1 of 14
No
No
Mmus\Setbp1
1 of 14
No
No
Mmus\Setd1a
1 of 14
No
No
Mmus\Setd2
1 of 14
No
No
Mmus\Setmar
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (12)
Xtro\setdb1
8 of 13
Yes
Yes
Xtro\setdb2
5 of 13
No
Yes
Xtro\suv39h2
2 of 13
No
No
Xtro\arid3b
1 of 13
No
No
Xtro\ehmt2
1 of 13
No
No
Xtro\fhip1b
1 of 13
No
No
Xtro\LOC594890
1 of 13
No
No
Xtro\LOC100488563
1 of 13
No
Yes
Xtro\LOC100490508
1 of 13
No
No
Xtro\LOC116408207
1 of 13
No
No
Xtro\phax
1 of 13
No
No
Xtro\setmar
1 of 13
No
No
Danio rerio (Zebrafish) (19)
Drer\setdb1a
11 of 14
Yes
Yes
Drer\setdb1b
10 of 14
No
Yes
Drer\setdb2
3 of 14
No
Yes
Drer\suv39h1a
2 of 14
No
No
Drer\suv39h1b
2 of 14
No
No
Drer\ehmt1a
1 of 14
No
No
Drer\ehmt1b
1 of 14
No
No
Drer\ehmt2
1 of 14
No
No
Drer\ezh1
1 of 14
No
No
Drer\kmt2a
1 of 14
No
No
Drer\kmt2ca
1 of 14
No
No
Drer\kmt2cb
1 of 14
No
No
Drer\LOC101883274
1 of 14
No
No
Drer\LOC103910708
1 of 14
No
Yes
Drer\nsd1a
1 of 14
No
No
Drer\nsd1b
1 of 14
No
No
Drer\nsd2
1 of 14
No
No
Drer\setbp1
1 of 14
No
No
Drer\setd1a
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (2)
Cele\met-2
9 of 14
Yes
Yes
Cele\set-11
1 of 14
No
No
Anopheles gambiae (African malaria mosquito) (4)
Agam\AgaP_AGAP007978
12 of 12
Yes
Yes
Agam\AgaP_AGAP003597
2 of 12
No
No
Agam\AgaP_AGAP002246
1 of 12
No
No
Agam\AgaP_AGAP003013
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (13)
Atha\SUVH6
3 of 13
Yes
No
Atha\SUVH1
2 of 13
No
No
Atha\SUVH5
2 of 13
No
No
Atha\EFS
1 of 13
No
No
Atha\SDG21
1 of 13
No
No
Atha\SUVH2
1 of 13
No
No
Atha\SUVH3
1 of 13
No
No
Atha\SUVH4
1 of 13
No
No
Atha\SUVH7
1 of 13
No
No
Atha\SUVH9
1 of 13
No
No
Atha\SUVR1
1 of 13
No
No
Atha\SUVR2
1 of 13
No
No
Atha\SUVR4
1 of 13
No
No
Saccharomyces cerevisiae (Brewer's yeast) (0)
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\clr4
1 of 12
Yes
No
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:egg. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (9)
Su(var)3-9
3 of 13
G9a
2 of 13
ash1
1 of 13
CG4565
1 of 13
Set1
1 of 13
Set2
1 of 13
Set8
1 of 13
trr
1 of 13
trx
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    SETDB1; SET domain bifurcated histone lysine methyltransferase 1
    12 of 14
    604396
         
        SETDB2; SET domain bifurcated histone lysine methyltransferase 2
        6 of 14
        607865
            Functional Complementation Data
            Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
            Interactions
            Summary of Physical Interactions
            Interaction Browsers
            View in FlyBase Interactions Browser   View in MIST tool (external link)
            Please see the Physical Interaction reports below for full details
            protein-protein
            Physical Interaction
            Assay
            References
            egg - ADD1
            anti tag coimmunoprecipitation, peptide massfingerprinting
            (Alekseyenko et al., 2014)
            egg - AMPdeam
            two hybrid, pull down, autoradiography
            (Stabell et al., 2006)
            egg - CG14464
            experimental knowledge based
            (Guruharsha et al., 2011)
            egg - CG14820
            two hybrid
            (Stabell et al., 2006)
            egg - CG8478
            two hybrid array
            (Shokri et al., 2019)
            egg - HP1b
            pull down, anti tag western blot
            (Lee et al., 2019)
            egg - HP1c
            pull down, anti tag western blot
            (Lee et al., 2019)
            egg - His3
            x-ray crystallography, enzymatic study, western blot, autoradiography, surface plasmon resonance, predetermined participant
            (Tang et al., 2024, Gou et al., 2010)
            egg - His4
            x-ray crystallography, surface plasmon resonance, predetermined participant
            (Tang et al., 2024)
            egg - Hr78
            two hybrid array
            (Shokri et al., 2019)
            egg - MEP-1
            anti bait coimmunoprecipitation, western blot
            (Mugat et al., 2020)
            egg - Mcr
            two hybrid
            (Stabell et al., 2006)
            egg - Pof
            anti tag coimmunoprecipitation, anti tag western blot
            (Tzeng et al., 2007)
            egg - SERCA
            two hybrid
            (Stabell et al., 2006)
            egg - Su(var)2-10
            anti tag coimmunoprecipitation, anti tag western blot, western blot, luminescence based mammalian interactome mapping
            (Mugat et al., 2020, Ninova et al., 2020)
            egg - Su(var)205
            pull down, anti tag western blot, phenotype-based detection assay, fluorescence microscopy, inferred by author, anti tag coimmunoprecipitation, peptide massfingerprinting
            (Lee et al., 2019, Alekseyenko et al., 2014, Hines et al., 2009)
            egg - Sumo
            anti tag coimmunoprecipitation, anti tag western blot
            (Ninova et al., 2020)
            egg - Ubc10
            two hybrid
            (Stabell et al., 2006)
            egg - Zasp52
            pull down, autoradiography, two hybrid
            (Stabell et al., 2006)
            egg - bon
            anti tag coimmunoprecipitation, anti tag western blot
            (Godneeva et al., 2023)
            egg - croc
            anti tag coimmunoprecipitation, anti tag western blot
            (Tu et al., 2020)
            egg - ctp
            two hybrid
            (Stabell et al., 2006)
            egg - drn
            two hybrid array
            (Shokri et al., 2019)
            egg - spir
            pull down, autoradiography, two hybrid
            (Stabell et al., 2006)
            egg - stwl
            two hybrid array
            (Shokri et al., 2019)
            egg - usp
            two hybrid
            (Stabell et al., 2006)
            egg - wde
            anti bait coimmunoprecipitation, anti tag western blot, anti tag coimmunoprecipitation, experimental knowledge based, western blot
            (Osumi et al., 2019, Seller et al., 2019, Guruharsha et al., 2011, Koch et al., 2009)
            Summary of Genetic Interactions
            Interaction Browsers
            View in FlyBase Interactions Browser   View in MIST tool (external link)
            Please look at the allele data for full details of the genetic interactions
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            egg
            enhanceable
            G9a
            (Brower-Toland et al., 2009)
            egg
            suppressible
            Su(var)3-9
            (Brower-Toland et al., 2009)
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            Su(var)205
            suppressible
            egg
            (Hines et al., 2009)
            External Data
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            DroID - A comprehensive database of gene and protein interactions.
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Pathways
            Signaling Pathways (FlyBase)
            Metabolic Pathways
            FlyBase
            External Links
            KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.
            External Data
            Linkouts
            KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            Class of Gene
            Genomic Location and Detailed Mapping Data
            Chromosome (arm)
            2R
            Recombination map
            2-107
            Cytogenetic map
            60E1-60E1
            Sequence location
            FlyBase Computed Cytological Location
            Cytogenetic map
            Evidence for location
            60E1-60E1
            Limits computationally determined from genome sequence between P{lacW}Mmp1k04809 and P{EP}Reg-5EP1065
            Experimentally Determined Cytological Location
            Cytogenetic map
            Notes
            References
            Experimentally Determined Recombination Data
            Location

            2-107

            (FlyBase, 2016)

            2-111.4

            (Comeron et al., 2012)
            Left of (cM)
            Right of (cM)
            Notes
            Stocks and Reagents
            Stocks (21)
            Genomic Clones (24)
            cDNA Clones (22)
             

            Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

            cDNA clones, fully sequenced
            BDGP DGC clones
            Other clones
              Drosophila Genomics Resource Center cDNA clones

              For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

              cDNA Clones, End Sequenced (ESTs)
              BDGP DGC clones
              Other clones
              RNAi and Array Information
              Linkouts
              DRSC - Results frm RNAi screens
              Antibody Information
              Laboratory Generated Antibodies
               

              monoclonal

              (Murano et al., 2019, Osumi et al., 2019, Gou et al., 2010)

              polyclonal

              (Sienski et al., 2015, Chiolo et al., 2011, Yoon et al., 2008)
              Commercially Available Antibodies
               
              Cell Line Information
              Publicly Available Cell Lines
               
                Other Stable Cell Lines
                 
                Other Comments

                egg is required in the ovary for trimethylation of His3 at its K9 residue.

                (Clough et al., 2007)

                egg is required for for the proliferation and viability of somatic cells in the germarium of the ovary. egg is also required for the viability of germ cells in the ovary.

                (Clough et al., 2007)

                Identification: In a genetic screen for EMS-induced lethal and female sterile mutations uncovered by Df(2R)Dll-MP.

                (Clough et al., 2006)

                egg mediates trimethylation of histone H3 at lysine 9 in ovary germ cells.

                (Clough et al., 2006)
                Relationship to Other Genes
                Source for database merge of

                Source for merge of: CG30426 BcDNA:AT13877

                (FlyBase, 1996-)

                Source for merge of: CG30422 CG30426

                (Hazelrigg, 2006.3.6)
                Additional comments

                Annotations CG30422 and CG30426 merged as CG12196 in release 5.2 of the genome annotation.

                (FlyBase Genome Annotators, 2007)

                FlyBase curator comment: Correspondence with author Lee revealed that 'dSETDB1' corresponds to 'egg'.

                (Lee et al., 2007)

                Source for merge of CG30426 BcDNA:AT13877 was shared cDNA (AT13877) ( date:030602 ).

                (FlyBase Genome Annotators, 2002-2003, FlyBase, 1996-)
                Nomenclature History
                Source for database identify of

                Source for identity of: egg CG30426

                (FlyBase Genome Annotators, 2007)

                Source for identity of: egg Dmsetdb1

                (Seum, 2007.2.26)

                Source for identity of: egg CG30422 CG30426

                (Hazelrigg, 2006.3.6)
                Nomenclature comments
                Etymology
                Synonyms and Secondary IDs (25)
                Reported As
                Symbol Synonym
                AAF47268 Dm
                (Alvarez-Venegas and Avramova, 2002)
                AAM70794
                (Hendrich and Tweedie, 2003)
                BcDNA:AT13877
                CG12196
                (National Institute of Genetics Fly Stocks, 2022-, Çiçek et al., 2016, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Ni et al., 2008, FlyBase Genome Annotators, 2007, Tzeng et al., 2007)
                CG30422
                (Clough et al., 2007, Seum, 2007.2.26, Seum et al., 2007, Tzeng et al., 2007)
                CG30426
                (Clough et al., 2007, Koh et al., 2007, Seum, 2007.2.26, Seum et al., 2007, Tzeng et al., 2007, Mis et al., 2006, Schotta et al., 2004, FlyBase, 1996-)
                Dm-setdb1
                (Seum et al., 2007)
                DmSETDB1
                (Shimaji et al., 2019)
                DmSetdb1
                (Huang et al., 2008, Seum et al., 2007, Seum et al., 2007)
                Dmsetdb1
                (Seum, 2007.2.26)
                EGG
                (Liu et al., 2020, Demidova et al., 2016, Alekseyenko et al., 2014, Riddle et al., 2012)
                Egg
                (Bernués et al., 2022, Kang et al., 2022, Casale et al., 2021, Eastwood et al., 2021, Onishi et al., 2021, Schnabl et al., 2021, Vidaurre and Chen, 2021, Kelleher et al., 2020, Onishi et al., 2020, Sato and Siomi, 2020, Tu et al., 2020, Hirakata and Siomi, 2019, Osumi et al., 2019, Amaral et al., 2017, Rogers et al., 2017, Hirakata and Siomi, 2016, Guzzardo et al., 2013, Koch et al., 2008)
                Eggless
                (Greer and Shi, 2012)
                SETDB1
                (Sarkar et al., 2023, Blatt et al., 2020, Benner et al., 2019, Maksimov and Koryakov, 2019, Paddibhatla et al., 2019, Mehrotra and Deshpande, 2018, Mani and Juliano, 2013, Stenberg and Larsson, 2011, Gou et al., 2010, Seum et al., 2007)
                SetDB1
                (Tang et al., 2024, Cabrita and Martinho, 2023, Godneeva et al., 2023, Melnikova and Golovnin, 2023, Cacchione et al., 2020, Gamez et al., 2020, Luo et al., 2020, Mugat et al., 2020, Ninova et al., 2020, Shimaji et al., 2019, Amaral et al., 2017, Caridi et al., 2017, Chen and Aravin, 2015, Sienski et al., 2015, Ost et al., 2014, Phalke et al., 2009)
                Setdb1
                (Lundberg et al., 2013, Figueiredo et al., 2012, Stenberg and Larsson, 2011)
                dEset
                (Hines et al., 2009, Stabell et al., 2006)
                dSETDB1
                (Lee et al., 2019, Masuko et al., 2018, Timms et al., 2016, Alekseyenko et al., 2014, Clough et al., 2014, Debruhl et al., 2013, Gou et al., 2010, Lee et al., 2010, Brower-Toland et al., 2009, Hines et al., 2009, Koch et al., 2009, Yoon et al., 2008, Lee et al., 2007, Tzeng et al., 2007, Tzeng et al., 2007, Mis et al., 2006)
                dSetDB1
                (Wang and Lin, 2021, Clémot et al., 2018)
                dSetdb1
                (Cryderman et al., 2011)
                dsetdb1
                (Yoon et al., 2008)
                egg
                (Kagemann et al., 2025, Spradling, 2024, Marzullo et al., 2023, Pang et al., 2023, Andreyeva et al., 2022, Eickelberg et al., 2022, Kalashnikova et al., 2021, Makki and Meller, 2021, Slaidina et al., 2021, Yamaguchi et al., 2021, Gulati et al., 2020, Meyer-Nava et al., 2020, Saint-Leandre et al., 2020, Tu et al., 2020, Gao et al., 2019, Maksimov and Koryakov, 2019, Seller et al., 2019, Shokri et al., 2019, Gene Disruption Project members, 2018-, Jankovics et al., 2018, Kang et al., 2018, Mehrotra and Deshpande, 2018, Song et al., 2018, Anreiter et al., 2017, Cheng et al., 2017, Dobson et al., 2017, Teixeira et al., 2017, Transgenic RNAi Project members, 2017-, Yang and Xi, 2017, Abramov et al., 2016, Dietz et al., 2015, Schertel et al., 2015, Yu et al., 2015, Clough et al., 2014, Ost et al., 2014, Wang et al., 2014, Yan et al., 2014, Sentmanat et al., 2013, Butchar et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Riddle et al., 2012, Friedman et al., 2011, Koryakov et al., 2011, Wang et al., 2011, Gan et al., 2010, Brower-Toland et al., 2009, Hines et al., 2009, Koch et al., 2009, Yoon et al., 2008, Clough and Hazelrigg, 2007, Clough et al., 2007, Nurminsky, 2007)
                setdb1
                (Shapiro-Kulnane et al., 2022)
                Name Synonyms
                Eggless
                (Kelleher et al., 2020, Murano et al., 2019, Zhao et al., 2019, Yamashiro and Siomi, 2018, Giauque and Bickel, 2016, Hirakata and Siomi, 2016, Clough et al., 2014, Zhang et al., 2014, Eliazer and Buszczak, 2011, Koch et al., 2009, Koch et al., 2008)
                Histone-lysine N-methyltransferase
                (Anholt and Williams, 2010)
                dSETDB1
                (Mis et al., 2006)
                eggless
                (Gibert and Peronnet, 2021, Clémot et al., 2018, Hughes et al., 2018, Mehrotra and Deshpande, 2018, Marie et al., 2017, Specchia et al., 2017, Chen and Aravin, 2015, Sienski et al., 2015, Handler et al., 2013, Eliazer and Buszczak, 2011, Brower-Toland et al., 2009, Hines et al., 2009, Clough et al., 2007, Nurminsky, 2007, Clough et al., 2006, Hazelrigg, 2006.3.6)
                Secondary FlyBase IDs
                • FBgn0065010
                • FBgn0050422
                • FBgn0035061
                • FBgn0050426
                Datasets (3)
                Study focus (3)
                Experimental Role
                Project
                Project Type
                Title
                • RNAi_target
                modENCODE_chromatin_proteins_cells
                Genome-wide localization of chromosomal proteins in cell lines by ChIP-chip and ChIP-Seq.
                • allele_used
                modENCODE_chromatin_proteins_flies
                Genome-wide localization of chromosomal proteins in fly tissues by ChIP-chip and ChIP-Seq.
                • allele_used
                modENCODE_chromatin_histones_flies
                Genome-wide localization of histones and their modifications in fly tissues by ChIP-chip and ChIP-Seq.
                Study result (0)
                Result
                Result Type
                Title
                External Crossreferences and Linkouts ( 53 )
                Sequence Crossreferences
                NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
                Other crossreferences
                AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                FlyMine - An integrated database for Drosophila genomics
                InterPro - A database of protein families, domains and functional sites
                KEGG Genes - Molecular building blocks of life in the genomic space.
                MARRVEL_MODEL - MARRVEL (model organism gene)
                PDB - An information portal to biological macromolecular structures
                Linkouts
                BioGRID - A database of protein and genetic interactions.
                Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                DroID - A comprehensive database of gene and protein interactions.
                DRSC - Results frm RNAi screens
                Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                Flygut - An atlas of the Drosophila adult midgut
                FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
                iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
                KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.
                KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
                MIST (protein-protein) - An integrated Molecular Interaction Database
                Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                References (200)