Gene Dmel\rg

Experimentally Determined Recombination Data

Location

1-11.0

(Curry, 1939)

1-2

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\rg for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

rg-RB

FBtr0070745

NM_167028

10795

3522

rg-RC

FBtr0110993

NM_001042796

11519

3712

rg-RD

FBtr0290088

NM_001144685

11549

3722

rg-RE

FBtr0299588

NM_001144686

11161

3578

rg-RF

FBtr0299589

NM_001144687

10898

3505

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

rg-PB

FBpp0070713

389.6

3522

5.31

NP_726978

AAN09135

rg-PC

FBpp0110292

411.0

3712

5.45

NP_001036261

ABI30968

rg-PD

FBpp0288527

412.3

3722

5.45

NP_001138157

ACL82890

rg-PE

FBpp0288863

395.5

3578

5.41

NP_001138158

AAF46011

rg-PF

FBpp0288864

391.0

3505

5.47

NP_001138159

ACL82891

Additional Polypeptide Data & Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

WD40-repeat-containing domain (IPR017986)

BEACH domain (IPR000409)

WD40 repeat (IPR001680)

Concanavalin A-like lectin/glucanases superfamily (IPR008985)

Domain of unknown function DUF1088 (IPR010508)

WD40/YVTN repeat-like-containing domain (IPR015943)

PH-BEACH domain (IPR023362)

Armadillo-type fold (IPR016024)

Concanavalin A-like lectin/glucanase, subgroup (IPR013320)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9W4E2

UniProtKB/TrEMBL

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Crossreferences

Expression Data

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment:maternally deposited

embryonic stage 4 -- 6

organism

embryonic stage 9 -- 10

head mesoderm

embryonic stage 11 -- 12

hindgut proper primordium

embryonic stage 13 -- 16

embryonic brain

embryonic central brain

embryonic central brain glial cell

embryonic esophagus

embryonic foregut

embryonic hindgut

embryonic proventriculus

embryonic/larval midgut

embryonic/larval nervous system

lateral cord glial cell

sensory nervous system primordium

ventral nerve cord

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

neuron of adult brain

• cell body

neuron of embryonic/larval brain

• cell body

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Wasbrough et al., 2010)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

Additional Descriptive Data

rg protein is detected in the cell bodies in the larval and adult brain, opposite to the cis-Golgi marker GM130.

Marker for

Subcellular Localization (GO Cellular Component)

CV term

References

cytoplasm

inferred from direct assay

(assigned by UniProtKB)

integral to membrane

inferred from direct assay

(assigned by UniProtKB)

neuronal cell body

inferred from direct assay

(The modENCODE Consortium, 2010, Graveley et al., 2011)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression in all larval and adult organs/tissues ranges from low to undetected. Expression at high levels in the following post-embryonic organs or tissues: adult eye, adult central nervous system. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval central nervous system, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, larval/adult salivary gland, larval trachea, adult male accessory gland.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		9
central nervous system, larvae L3		28
central nervous system, pupae P8		44
head, virgin 1-day female		16
head, virgin 4-day female		13
head, virgin 20-day female		10
head, mated 1-day female		15
head, mated 4-day female		14
head, mated 20-day female		9
head, mated 1-day male		28
head, mated 4-day male		23
head, mated 20-day male		18
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		3
digestive system, larvae L3 wandering		4
digestive system, 1-day adult		6
digestive system, 4-day adult		8
digestive system, 20-day adult		7
fat body, larvae L3 wandering		0
fat body, white prepupae		2
fat body, pupae P8		9
carcass, larvae L3 wandering		11
carcass, 1-day adult		4
carcass, 4-day adult		3
carcass, 20-day adult		2
ovary, virgin 4-day female		8
ovary, mated 4-day female		8
testis, mated 4-day male		1
accessory gland, mated 4-day male		8
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		9
central nervous system, larvae L3		(28)
central nervous system, pupae P8		(44)
head, virgin 1-day female		16
head, virgin 4-day female		13
head, virgin 20-day female		10
head, mated 1-day female		15
head, mated 4-day female		14
head, mated 20-day female		9
head, mated 1-day male		(28)
head, mated 4-day male		23
head, mated 20-day male		18
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		3
digestive system, larvae L3 wandering		4
digestive system, 1-day adult		6
digestive system, 4-day adult		8
digestive system, 20-day adult		7
fat body, larvae L3 wandering		0
fat body, white prepupae		2
fat body, pupae P8		9
carcass, larvae L3 wandering		11
carcass, 1-day adult		4
carcass, 4-day adult		3
carcass, 20-day adult		2
ovary, virgin 4-day female		8
ovary, mated 4-day female		8
testis, mated 4-day male		1
accessory gland, mated 4-day male		8
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		9
central nervous system, larvae L3		28
central nervous system, pupae P8		44
head, virgin 1-day female		16
head, virgin 4-day female		13
head, virgin 20-day female		10
head, mated 1-day female		15
head, mated 4-day female		14
head, mated 20-day female		9
head, mated 1-day male		28
head, mated 4-day male		23
head, mated 20-day male		18
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		3
digestive system, larvae L3 wandering		4
digestive system, 1-day adult		6
digestive system, 4-day adult		8
digestive system, 20-day adult		7
fat body, larvae L3 wandering		0
fat body, white prepupae		2
fat body, pupae P8		9
carcass, larvae L3 wandering		11
carcass, 1-day adult		4
carcass, 4-day adult		3
carcass, 20-day adult		2
ovary, virgin 4-day female		8
ovary, mated 4-day female		8
testis, mated 4-day male		1
accessory gland, mated 4-day male		8
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		9
central nervous system, larvae L3		28
central nervous system, pupae P8		44
head, virgin 1-day female		16
head, virgin 4-day female		13
head, virgin 20-day female		10
head, mated 1-day female		15
head, mated 4-day female		14
head, mated 20-day female		9
head, mated 1-day male		28
head, mated 4-day male		23
head, mated 20-day male		18
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		3
digestive system, larvae L3 wandering		4
digestive system, 1-day adult		6
digestive system, 4-day adult		8
digestive system, 20-day adult		7
fat body, larvae L3 wandering		0
fat body, white prepupae		2
fat body, pupae P8		9
carcass, larvae L3 wandering		11
carcass, 1-day adult		4
carcass, 4-day adult		3
carcass, 20-day adult		2
ovary, virgin 4-day female		8
ovary, mated 4-day female		8
testis, mated 4-day male		1
accessory gland, mated 4-day male		8
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		9
central nervous system, larvae L3		28
central nervous system, pupae P8		44
head, virgin 1-day female		16
head, virgin 4-day female		13
head, virgin 20-day female		10
head, mated 1-day female		15
head, mated 4-day female		14
head, mated 20-day female		9
head, mated 1-day male		28
head, mated 4-day male		23
head, mated 20-day male		18
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		3
digestive system, larvae L3 wandering		4
digestive system, 1-day adult		6
digestive system, 4-day adult		8
digestive system, 20-day adult		7
fat body, larvae L3 wandering		0
fat body, white prepupae		2
fat body, pupae P8		9
carcass, larvae L3 wandering		11
carcass, 1-day adult		4
carcass, 4-day adult		3
carcass, 20-day adult		2
ovary, virgin 4-day female		8
ovary, mated 4-day female		8
testis, mated 4-day male		1
accessory gland, mated 4-day male		8
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		9
central nervous system, larvae L3		28
central nervous system, pupae P8		(44)
head, virgin 1-day female		16
head, virgin 4-day female		13
head, virgin 20-day female		10
head, mated 1-day female		15
head, mated 4-day female		14
head, mated 20-day female		9
head, mated 1-day male		28
head, mated 4-day male		23
head, mated 20-day male		18
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		3
digestive system, larvae L3 wandering		4
digestive system, 1-day adult		6
digestive system, 4-day adult		8
digestive system, 20-day adult		7
fat body, larvae L3 wandering		0
fat body, white prepupae		2
fat body, pupae P8		9
carcass, larvae L3 wandering		11
carcass, 1-day adult		4
carcass, 4-day adult		3
carcass, 20-day adult		2
ovary, virgin 4-day female		8
ovary, mated 4-day female		8
testis, mated 4-day male		1
accessory gland, mated 4-day male		8
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		9
central nervous system, larvae L3		28
central nervous system, pupae P8		44
head, virgin 1-day female		16
head, virgin 4-day female		13
head, virgin 20-day female		10
head, mated 1-day female		15
head, mated 4-day female		14
head, mated 20-day female		9
head, mated 1-day male		28
head, mated 4-day male		23
head, mated 20-day male		18
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		3
digestive system, larvae L3 wandering		4
digestive system, 1-day adult		6
digestive system, 4-day adult		8
digestive system, 20-day adult		7
fat body, larvae L3 wandering		0
fat body, white prepupae		2
fat body, pupae P8		9
carcass, larvae L3 wandering		11
carcass, 1-day adult		4
carcass, 4-day adult		3
carcass, 20-day adult		2
ovary, virgin 4-day female		8
ovary, mated 4-day female		8
testis, mated 4-day male		1
accessory gland, mated 4-day male		8
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		9
central nervous system, larvae L3		28
central nervous system, pupae P8		44
head, virgin 1-day female		16
head, virgin 4-day female		13
head, virgin 20-day female		10
head, mated 1-day female		15
head, mated 4-day female		14
head, mated 20-day female		9
head, mated 1-day male		28
head, mated 4-day male		23
head, mated 20-day male		18
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		3
digestive system, larvae L3 wandering		4
digestive system, 1-day adult		6
digestive system, 4-day adult		8
digestive system, 20-day adult		7
fat body, larvae L3 wandering		0
fat body, white prepupae		2
fat body, pupae P8		9
carcass, larvae L3 wandering		11
carcass, 1-day adult		4
carcass, 4-day adult		3
carcass, 20-day adult		2
ovary, virgin 4-day female		8
ovary, mated 4-day female		8
testis, mated 4-day male		1
accessory gland, mated 4-day male		8
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 12-24 hour embryonic stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		5
embryo 02-04hr		4
embryo 04-06hr		2
embryo 06-08hr		3
embryo 08-10hr		6
embryo 10-12hr		9
embryo 12-14hr		16
embryo 14-16hr		24
embryo 16-18hr		22
embryo 18-20hr		19
embryo 20-22hr		14
embryo 22-24hr		12
larva L1		17
larva L2		11
larva L3 12hr old		8
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		9
larva L3 puffstage 7-9		8
white prepupae new		8
white prepupae 12hr		9
white prepupae 24hr		13
pupae 2d postWPP		12
pupae 3d postWPP		10
pupae 4d postWPP		7
adult male 01day		9
adult male 05day		10
adult male 30day		10
adult female 01day		8
adult female 05day		7
adult female 30day		6
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		5
embryo 02-04hr		4
embryo 04-06hr		2
embryo 06-08hr		3
embryo 08-10hr		6
embryo 10-12hr		9
embryo 12-14hr		16
embryo 14-16hr		24
embryo 16-18hr		22
embryo 18-20hr		19
embryo 20-22hr		14
embryo 22-24hr		12
larva L1		17
larva L2		11
larva L3 12hr old		8
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		9
larva L3 puffstage 7-9		8
white prepupae new		8
white prepupae 12hr		9
white prepupae 24hr		13
pupae 2d postWPP		12
pupae 3d postWPP		10
pupae 4d postWPP		7
adult male 01day		9
adult male 05day		10
adult male 30day		10
adult female 01day		8
adult female 05day		7
adult female 30day		6
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		5
embryo 02-04hr		4
embryo 04-06hr		2
embryo 06-08hr		3
embryo 08-10hr		6
embryo 10-12hr		9
embryo 12-14hr		16
embryo 14-16hr		24
embryo 16-18hr		22
embryo 18-20hr		19
embryo 20-22hr		14
embryo 22-24hr		12
larva L1		17
larva L2		11
larva L3 12hr old		8
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		9
larva L3 puffstage 7-9		8
white prepupae new		8
white prepupae 12hr		9
white prepupae 24hr		13
pupae 2d postWPP		12
pupae 3d postWPP		10
pupae 4d postWPP		7
adult male 01day		9
adult male 05day		10
adult male 30day		10
adult female 01day		8
adult female 05day		7
adult female 30day		6
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		5
embryo 02-04hr		4
embryo 04-06hr		2
embryo 06-08hr		3
embryo 08-10hr		6
embryo 10-12hr		9
embryo 12-14hr		16
embryo 14-16hr		24
embryo 16-18hr		22
embryo 18-20hr		19
embryo 20-22hr		14
embryo 22-24hr		12
larva L1		17
larva L2		11
larva L3 12hr old		8
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		9
larva L3 puffstage 7-9		8
white prepupae new		8
white prepupae 12hr		9
white prepupae 24hr		13
pupae 2d postWPP		12
pupae 3d postWPP		10
pupae 4d postWPP		7
adult male 01day		9
adult male 05day		10
adult male 30day		10
adult female 01day		8
adult female 05day		7
adult female 30day		6
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		5
embryo 02-04hr		4
embryo 04-06hr		2
embryo 06-08hr		3
embryo 08-10hr		6
embryo 10-12hr		9
embryo 12-14hr		16
embryo 14-16hr		24
embryo 16-18hr		22
embryo 18-20hr		19
embryo 20-22hr		14
embryo 22-24hr		12
larva L1		17
larva L2		11
larva L3 12hr old		8
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		9
larva L3 puffstage 7-9		8
white prepupae new		8
white prepupae 12hr		9
white prepupae 24hr		13
pupae 2d postWPP		12
pupae 3d postWPP		10
pupae 4d postWPP		7
adult male 01day		9
adult male 05day		10
adult male 30day		10
adult female 01day		8
adult female 05day		7
adult female 30day		6
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		5
embryo 02-04hr		4
embryo 04-06hr		2
embryo 06-08hr		3
embryo 08-10hr		6
embryo 10-12hr		9
embryo 12-14hr		16
embryo 14-16hr		24
embryo 16-18hr		22
embryo 18-20hr		19
embryo 20-22hr		14
embryo 22-24hr		12
larva L1		17
larva L2		11
larva L3 12hr old		8
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		9
larva L3 puffstage 7-9		8
white prepupae new		8
white prepupae 12hr		9
white prepupae 24hr		13
pupae 2d postWPP		12
pupae 3d postWPP		10
pupae 4d postWPP		7
adult male 01day		9
adult male 05day		10
adult male 30day		10
adult female 01day		8
adult female 05day		7
adult female 30day		6
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		5
embryo 02-04hr		4
embryo 04-06hr		2
embryo 06-08hr		3
embryo 08-10hr		6
embryo 10-12hr		9
embryo 12-14hr		16
embryo 14-16hr		24
embryo 16-18hr		22
embryo 18-20hr		19
embryo 20-22hr		14
embryo 22-24hr		12
larva L1		17
larva L2		11
larva L3 12hr old		8
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		9
larva L3 puffstage 7-9		8
white prepupae new		8
white prepupae 12hr		9
white prepupae 24hr		13
pupae 2d postWPP		12
pupae 3d postWPP		10
pupae 4d postWPP		7
adult male 01day		9
adult male 05day		10
adult male 30day		10
adult female 01day		8
adult female 05day		7
adult female 30day		6
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		5
embryo 02-04hr		4
embryo 04-06hr		2
embryo 06-08hr		3
embryo 08-10hr		6
embryo 10-12hr		9
embryo 12-14hr		16
embryo 14-16hr		24
embryo 16-18hr		22
embryo 18-20hr		19
embryo 20-22hr		14
embryo 22-24hr		12
larva L1		17
larva L2		11
larva L3 12hr old		8
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		9
larva L3 puffstage 7-9		8
white prepupae new		8
white prepupae 12hr		9
white prepupae 24hr		13
pupae 2d postWPP		12
pupae 3d postWPP		10
pupae 4d postWPP		7
adult male 01day		9
adult male 05day		10
adult male 30day		10
adult female 01day		8
adult female 05day		7
adult female 30day		6
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		8
embryonic 1182-4H		1
embryonic GM2		0
embryonic Kc167		0
embryonic S1		4
embryonic S3		0
leg disc CME L1		8
wing disc CME-W2		2
wing disc ML-DmD8		6
wing disc ML-DmD9		5
wing disc ML-DmD16-c3		1
wing disc ML-DmD21		0
wing disc ML-DmD32		1
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		65
antennal disc ML-DmD20-c5		1
mixed discs ML-DmD4-c1		1
CNS ML-DmBG1-c1		1
CNS ML-DmBG2-c2		3
tumorous blood cells mbn2		0
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		4
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		8
embryonic 1182-4H		1
embryonic GM2		0
embryonic Kc167		0
embryonic S1		4
embryonic S3		0
leg disc CME L1		8
wing disc CME-W2		2
wing disc ML-DmD8		6
wing disc ML-DmD9		5
wing disc ML-DmD16-c3		1
wing disc ML-DmD21		0
wing disc ML-DmD32		1
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		(65)
antennal disc ML-DmD20-c5		1
mixed discs ML-DmD4-c1		1
CNS ML-DmBG1-c1		1
CNS ML-DmBG2-c2		3
tumorous blood cells mbn2		0
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		4
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		8
embryonic 1182-4H		1
embryonic GM2		0
embryonic Kc167		0
embryonic S1		4
embryonic S3		0
leg disc CME L1		8
wing disc CME-W2		2
wing disc ML-DmD8		6
wing disc ML-DmD9		5
wing disc ML-DmD16-c3		1
wing disc ML-DmD21		0
wing disc ML-DmD32		1
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		65
antennal disc ML-DmD20-c5		1
mixed discs ML-DmD4-c1		1
CNS ML-DmBG1-c1		1
CNS ML-DmBG2-c2		3
tumorous blood cells mbn2		0
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		4
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		8
embryonic 1182-4H		1
embryonic GM2		0
embryonic Kc167		0
embryonic S1		4
embryonic S3		0
leg disc CME L1		8
wing disc CME-W2		2
wing disc ML-DmD8		6
wing disc ML-DmD9		5
wing disc ML-DmD16-c3		1
wing disc ML-DmD21		0
wing disc ML-DmD32		1
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		65
antennal disc ML-DmD20-c5		1
mixed discs ML-DmD4-c1		1
CNS ML-DmBG1-c1		1
CNS ML-DmBG2-c2		3
tumorous blood cells mbn2		0
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		4
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		8
embryonic 1182-4H		1
embryonic GM2		0
embryonic Kc167		0
embryonic S1		4
embryonic S3		0
leg disc CME L1		8
wing disc CME-W2		2
wing disc ML-DmD8		6
wing disc ML-DmD9		5
wing disc ML-DmD16-c3		1
wing disc ML-DmD21		0
wing disc ML-DmD32		1
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		65
antennal disc ML-DmD20-c5		1
mixed discs ML-DmD4-c1		1
CNS ML-DmBG1-c1		1
CNS ML-DmBG2-c2		3
tumorous blood cells mbn2		0
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		4
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		8
embryonic 1182-4H		1
embryonic GM2		0
embryonic Kc167		0
embryonic S1		4
embryonic S3		0
leg disc CME L1		8
wing disc CME-W2		2
wing disc ML-DmD8		6
wing disc ML-DmD9		5
wing disc ML-DmD16-c3		1
wing disc ML-DmD21		0
wing disc ML-DmD32		1
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		(65)
antennal disc ML-DmD20-c5		1
mixed discs ML-DmD4-c1		1
CNS ML-DmBG1-c1		1
CNS ML-DmBG2-c2		3
tumorous blood cells mbn2		0
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		4
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		8
embryonic 1182-4H		1
embryonic GM2		0
embryonic Kc167		0
embryonic S1		4
embryonic S3		0
leg disc CME L1		8
wing disc CME-W2		2
wing disc ML-DmD8		6
wing disc ML-DmD9		5
wing disc ML-DmD16-c3		1
wing disc ML-DmD21		0
wing disc ML-DmD32		1
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		65
antennal disc ML-DmD20-c5		1
mixed discs ML-DmD4-c1		1
CNS ML-DmBG1-c1		1
CNS ML-DmBG2-c2		3
tumorous blood cells mbn2		0
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		4
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		0
Schneider line 2 Sg4		8
embryonic 1182-4H		1
embryonic GM2		0
embryonic Kc167		0
embryonic S1		4
embryonic S3		0
leg disc CME L1		8
wing disc CME-W2		2
wing disc ML-DmD8		6
wing disc ML-DmD9		5
wing disc ML-DmD16-c3		1
wing disc ML-DmD21		0
wing disc ML-DmD32		1
haltere disc ML-DmD17-c3		0
eye-antennal disc ML-DmD11		65
antennal disc ML-DmD20-c5		1
mixed discs ML-DmD4-c1		1
CNS ML-DmBG1-c1		1
CNS ML-DmBG2-c2		3
tumorous blood cells mbn2		0
ovary fGS/OSS		0
ovary OSC		0
ovary OSS		4
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		7
cold shock, 4-day adult		6
heat shock, 4-day adult		5
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		1
Cadmium 50 mM 48 hrs, 4-day adult		5
Cadmium 100 mM 48 hrs, 4-day adult		7
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		3
Zinc 5 mM 12 hrs, larvae L3		3
Zinc 4.5 mM 48 hrs, 4-day adult		5
Ethanol 2.5% 3 hrs, larvae L3		9
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		5
Caffeine 25 mg/ml 48 hrs, 4-day adult		5
Paraquat 5 mM 48 hrs, 4-day adult		6
Paraquat 10 mM 48 hrs, 4-day adult		9
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		7
cold shock, 4-day adult		6
heat shock, 4-day adult		5
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		1
Cadmium 50 mM 48 hrs, 4-day adult		5
Cadmium 100 mM 48 hrs, 4-day adult		7
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		3
Zinc 5 mM 12 hrs, larvae L3		3
Zinc 4.5 mM 48 hrs, 4-day adult		5
Ethanol 2.5% 3 hrs, larvae L3		9
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		5
Caffeine 25 mg/ml 48 hrs, 4-day adult		5
Paraquat 5 mM 48 hrs, 4-day adult		6
Paraquat 10 mM 48 hrs, 4-day adult		9
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		7
cold shock, 4-day adult		6
heat shock, 4-day adult		5
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		1
Cadmium 50 mM 48 hrs, 4-day adult		5
Cadmium 100 mM 48 hrs, 4-day adult		7
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		3
Zinc 5 mM 12 hrs, larvae L3		3
Zinc 4.5 mM 48 hrs, 4-day adult		5
Ethanol 2.5% 3 hrs, larvae L3		9
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		5
Caffeine 25 mg/ml 48 hrs, 4-day adult		5
Paraquat 5 mM 48 hrs, 4-day adult		6
Paraquat 10 mM 48 hrs, 4-day adult		9
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		7
cold shock, 4-day adult		6
heat shock, 4-day adult		5
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		1
Cadmium 50 mM 48 hrs, 4-day adult		5
Cadmium 100 mM 48 hrs, 4-day adult		7
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		3
Zinc 5 mM 12 hrs, larvae L3		3
Zinc 4.5 mM 48 hrs, 4-day adult		5
Ethanol 2.5% 3 hrs, larvae L3		9
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		5
Caffeine 25 mg/ml 48 hrs, 4-day adult		5
Paraquat 5 mM 48 hrs, 4-day adult		6
Paraquat 10 mM 48 hrs, 4-day adult		9
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		7
cold shock, 4-day adult		6
heat shock, 4-day adult		5
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		1
Cadmium 50 mM 48 hrs, 4-day adult		5
Cadmium 100 mM 48 hrs, 4-day adult		7
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		3
Zinc 5 mM 12 hrs, larvae L3		3
Zinc 4.5 mM 48 hrs, 4-day adult		5
Ethanol 2.5% 3 hrs, larvae L3		9
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		5
Caffeine 25 mg/ml 48 hrs, 4-day adult		5
Paraquat 5 mM 48 hrs, 4-day adult		6
Paraquat 10 mM 48 hrs, 4-day adult		9
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		7
cold shock, 4-day adult		6
heat shock, 4-day adult		5
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		1
Cadmium 50 mM 48 hrs, 4-day adult		5
Cadmium 100 mM 48 hrs, 4-day adult		7
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		3
Zinc 5 mM 12 hrs, larvae L3		3
Zinc 4.5 mM 48 hrs, 4-day adult		5
Ethanol 2.5% 3 hrs, larvae L3		9
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		5
Caffeine 25 mg/ml 48 hrs, 4-day adult		5
Paraquat 5 mM 48 hrs, 4-day adult		6
Paraquat 10 mM 48 hrs, 4-day adult		9
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		7
cold shock, 4-day adult		6
heat shock, 4-day adult		5
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		1
Cadmium 50 mM 48 hrs, 4-day adult		5
Cadmium 100 mM 48 hrs, 4-day adult		7
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		3
Zinc 5 mM 12 hrs, larvae L3		3
Zinc 4.5 mM 48 hrs, 4-day adult		5
Ethanol 2.5% 3 hrs, larvae L3		9
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		5
Caffeine 25 mg/ml 48 hrs, 4-day adult		5
Paraquat 5 mM 48 hrs, 4-day adult		6
Paraquat 10 mM 48 hrs, 4-day adult		9
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		7
cold shock, 4-day adult		6
heat shock, 4-day adult		5
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		1
Cadmium 50 mM 48 hrs, 4-day adult		5
Cadmium 100 mM 48 hrs, 4-day adult		7
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		3
Zinc 5 mM 12 hrs, larvae L3		3
Zinc 4.5 mM 48 hrs, 4-day adult		5
Ethanol 2.5% 3 hrs, larvae L3		9
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		5
Caffeine 25 mg/ml 48 hrs, 4-day adult		5
Paraquat 5 mM 48 hrs, 4-day adult		6
Paraquat 10 mM 48 hrs, 4-day adult		9
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

External Data & Images

Show all FlyExpress processed images for rg

BDGP expression data

•

GH23814

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0086911

FlyExpress - Embryonic expression images (BDGP data)

•

Stages(s) 9-10

Stages(s) 11-12

Stages(s) 13-16

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal | embryonic stage

partially lethal - majority die | recessive

rg^e8R

viable

viable, with Scer\GAL4^pnr-MD237

viable | reduced

Sterility

Allele

fertile

rg⁷

fertile | reduced

rg^P

Other Phenotypes

Allele

body color defective

rg^P

eclosion rhythm defective

rg⁷

learning defective

memory defective

rg¹

neuroanatomy defective | adult stage

neuroanatomy defective | adult stage (with Df(1)ovo41)

neuroanatomy defective | third instar larval stage

neuroanatomy defective | third instar larval stage (with Df(1)ovo41)

rg^FDD

visible

visible (with Df(1)JC70)

visible (with rg¹)

visible (with rg⁴¹)

rg¹

visible (with rg^γ1)

rg¹

visible (with rg^γ2)

rg¹

visible (with rg^γ3)

rg¹

visible (with rg^γ4)

rg¹

visible (with rg^γ5)

rg¹

visible (with rg^γ6)

rg¹

visible (with rg^γ7)

rg¹

visible (with rg^γ8)

rg¹

visible (with rg^γ9)

rg¹

visible (with rg^γ10)

rg¹

visible (with rg^γ11)

rg¹

Phenotype manifest in

Allele

adult mushroom body

adult mushroom body (with Df(1)ovo41)

cone cell

eye

eye (with Df(1)JC70)

eye (with rg¹)

eye (with rg⁴¹)

rg¹

eye (with rg^γ1)

rg¹

eye (with rg^γ2)

rg¹

eye (with rg^γ3)

rg¹

eye (with rg^γ4)

rg¹

eye (with rg^γ5)

rg¹

eye (with rg^γ6)

rg¹

eye (with rg^γ7)

rg¹

eye (with rg^γ8)

rg¹

eye (with rg^γ9)

rg¹

eye (with rg^γ10)

rg¹

eye (with rg^γ11)

rg¹

eye photoreceptor cell

rg^γ6

interommatidial bristle

rg^γ6

NMJ bouton | third instar larval stage

NMJ bouton | third instar larval stage (with Df(1)ovo4)

rg^FDD

ommatidium

pigment cell

rg^γ6

retina

rg^γ6

secondary pigment cell

rg^p5

wing

wing vein

rg^unspecified

wing vein L5

rg^unspecified

Classical Alleles ( 54 )

For All Classical Alleles Show

Allele of rg	Class	Mutagen	Stocks	Known lesion
rg⁰⁹			1	--
rg^e02307			1	--
rg^e03870			1	--
rg^e03909			1	--
rg^EY03995			1	--
rg^EY13849			1	--
rg^f02898			1	--
rg^f04040		piggyBac transposase	1	--
rg^f05791			1	--
rg^f07843			1	--
rg^f07969			1	--
rg^KG02343		P-element activity	1	--
rg^MB01845			1	--
rg^MB03792			1	--
rg^MB05724			1	--
rg^MI05253			1	--
rg^NP0965			1	--
rg^NP3467			1	--
rg^FDD	amorphic allele - molecular evidence	FLPase	0	Yes
rg⁰			0	--
rg¹	hypomorphic allele - molecular evidence, hypomorphic allele - genetic evidence		0	--
rg²			0	--
rg³³ⁱ⁹		heat-treatment	0	--
rg³		spontaneous	0	--
rg⁴¹	hypomorphic allele - genetic evidence	ethyl methanesulfonate	0	--
rg⁴		heat-treatment	0	--
rg⁵		spontaneous	0	--
rg⁶		spontaneous	0	--
rg⁷		X ray	0	--
rg^CPTI003323		piggyBac transposase	0	Yes
rg^e8R		ethyl methanesulfonate	0	--
rg^GG01099			0	--
rg^p1	hypomorphic allele - genetic evidence	Delta2-3	0	--
rg^p2	hypomorphic allele - molecular evidence	Delta2-3	0	--
rg^p3	loss of function allele	Delta2-3	0	--
rg^p4	hypomorphic allele - molecular evidence		0	--
rg^p5	loss of function allele	Delta2-3	0	--
rg^p6	hypomorphic allele - molecular evidence, hypomorphic allele - genetic evidence	Delta2-3	0	--
rg^p9	hypomorphic allele - genetic evidence	Delta2-3	0	--
rg^P		32P	0	--
rg^SP89		gamma ray	0	--
rg^unspecified			0	--
rg^x3			0	--
rg^γ10	hypomorphic allele - genetic evidence	gamma ray	0	--
rg^γ11		gamma ray	0	--
rg^γ1	loss of function allele, hypomorphic allele - molecular evidence	gamma ray	0	--
rg^γ2	hypomorphic allele - genetic evidence	gamma ray	0	--
rg^γ3	loss of function allele	gamma ray	0	--
rg^γ4	hypomorphic allele - genetic evidence	gamma ray	0	--
rg^γ5	hypomorphic allele - molecular evidence, hypomorphic allele - genetic evidence	gamma ray	0	--
rg^γ6	loss of function allele, hypomorphic allele - molecular evidence	gamma ray	0	--
rg^γ7	hypomorphic allele - genetic evidence	gamma ray	0	--
rg^γ8	hypomorphic allele - genetic evidence	gamma ray	0	--
rg^γ9	hypomorphic allele - genetic evidence	gamma ray	0	--

Alleles Carried on Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Allele of rg	Mutagen	Stocks	Known lesion
rg^GD10753	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
rg^KK102295	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
rg^GD14583	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes
rg^Scer\UAS.cVa	in vitro construct - regulatory fusion	0	Yes

Aneuploid Aberrations

Not disrupted in

Df(1)bi-D2

Df(1)bi-DL5

Df(1)C149

Df(1)cho23

Df(1)ct268-37

(Demerec, 1940)

Df(1)GA56

Df(1)GA102

Df(1)N73

Df(1)rb1

(Steinmann-Zwicky, 1988, Oliver et al., 1988)

Df(1)rb13

Df(1)rb29

Df(1)rb33

Df(1)rb35

Df(1)rb46

Df(1)rb47

Df(1)rb48

Df(1)w^m258-44

In(1)Cl^Lbb^DfR

Not duplicated in

In(1)bb^DfLCl^R

Disrupted in

Df(1)bi-DL3

Df(1)cho19

(Craymer and Roy, 1980, Steinmann-Zwicky, 1988, Oliver et al., 1988, Salz, 1992)

Df(1)DEB4D

(Salz, 1992)

Df(1)HC244

Df(1)HF366

(Craymer and Roy, 1980, Oliver et al., 1988)

Df(1)JC70

(Craymer and Roy, 1980, Oliver et al., 1988, Salz, 1992, Han et al., 1997)

Df(1)ovo4

Df(1)ovo6

(Oliver et al., 1988, Salz, 1992)

Df(1)ovo7

(Oliver et al., 1988, Salz, 1992)

Df(1)ovo14

Df(1)ovo15

Df(1)ovo41

Df(1)ovo44

(Steinmann-Zwicky, 1988, Salz, 1992)

Df(1)rb14

Df(1)rb30

(Craymer and Roy, 1980, Steinmann-Zwicky, 1988, Oliver et al., 1988, Salz, 1992)

Df(1)RC40

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD10753}

P{KK102295}

P{UAS-rg.V}

characterization construct

P{GD14583}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

Mi{MIC}rg^MI05253

P{SUPor-P}rg^KG02343

P{UASp-YFP.Rab21.Q73L}rg⁰⁹

PBac{754.P.FSVS-0}rg^CPTI003323

insertion of mobile activating element

P{EPgy2}rg^EY03995

P{EPgy2}rg^EY13849

P{Mae-UAS.6.11}rg^GG01099

insertion of enhancer trap binary system

insertion of enhancer trap

Gene Ontology: Function, Process & Cellular Component ( 9 unique terms )

Terms Based on Experimental Evidence ( 9 terms )

Molecular Function

CV term

References

protein kinase A binding

inferred from direct assay

(assigned by UniProtKB)

Biological Process

CV term

compound eye cone cell differentiation

References

inferred from mutant phenotype

mushroom body development

inferred from mutant phenotype

neuromuscular junction development

inferred from mutant phenotype

olfactory learning

inferred from mutant phenotype

protein localization

inferred from direct assay

(assigned by UniProtKB)

Cellular Component

CV term

References

cytoplasm

inferred from direct assay

(assigned by UniProtKB)

integral to membrane

inferred from direct assay

(assigned by UniProtKB)

neuronal cell body

inferred from direct assay

Terms Based on Predictions or Assertions ( 2 terms )

Molecular Function

CV term

References

protein kinase A binding

non-traceable author statement

(Han, 1997.5.12)

non-traceable author statement

(assigned by UniProtKB)

Biological Process

CV term

References

protein localization

non-traceable author statement

(Han, 1997.5.12)

non-traceable author statement

(assigned by UniProtKB)

(Mintseris et al., 2009.11.23)

Cellular Component ( 0 terms)

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

protein-protein

Interacting group

Assay

References

rg - CG4546

anti tag coimmunoprecipitation, peptide massfingerprinting

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

rg allele

Gene

References

rg^unspecified

aos

bul

soba

Su(H)

rg^γ1

bul

rg^γ3

rg^γ6

Egfr

Ras85D

rg^γ10

aos

External Data

BioGRID - A database of protein and genetic interactions

•

69112

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0086911

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

44531

SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores.

•

Q9W4E2

Orthologs

OrthoDB Orthologs (65) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG66Q7BS

Dipteran inclusive ortholog search

EOG6BZN9G

Insect inclusive ortholog search

EOG6HT7N1

Arthropod inclusive ortholog search

EOG637PVW

Metazoa inclusive ortholog search

EOG679T0V

Orthologs in Drosophila Species (EOG66Q7BS)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA19855

Drosophila pseudoobscura pseudoobscura

Dpse\GA13721

Drosophila persimilis

Dper\GL14283

Drosophila willistoni

Dwil\GK10290

Drosophila willistoni

Dwil\GK24149

Drosophila virilis

Dvir\GJ16846

Drosophila mojavensis

Dmoj\GI12711

Drosophila mojavensis

Dmoj\GI12327

Drosophila mojavensis

Dmoj\GI15273

Drosophila grimshawi

Dgri\GH24603

Drosophila grimshawi

Dgri\GH15669

Orthologs in non-Drosophila Dipterans (EOG6BZN9G)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL014730

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL003592

Anopheles gambiae

Malaria mosquito

Agam\AGAP000017

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ007145

Orthologs in non-Dipteran Insects (EOG6HT7N1)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\GB52925

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG013477

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH21641

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH21646

Atta cephalotes

Leafcutter ant

Acep\ACEP25717

Atta cephalotes

Leafcutter ant

Acep\ACEP25723

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO17389

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO17390

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL15079

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL15078

Linepithema humile

Argentine ant

Lhum\LH13791

Linepithema humile

Argentine ant

Lhum\LH13786

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB24818

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB24819

Solenopsis invicta

Red fire ant

Sinv\SINV24775

Solenopsis invicta

Red fire ant

Sinv\SINV24782

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI004514

Bombyx mori

Silkmoth

Bmor\BGIBMGA009317

Bombyx mori

Silkmoth

Bmor\BGIBMGA009316

Pediculus humanus

Human body louse

Phum\PHUM055020

Pediculus humanus

Human body louse

Phum\PHUM055000

Tribolium castaneum

Red flour beetle

Tcas\GLEAN_07593

Tribolium castaneum

Red flour beetle

Tcas\GLEAN_07591

Orthologs in non-Insect Arthropods (EOG637PVW)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\JGI_V11_319191

Ixodes scapularis

Deer tick

Isca\ISCW018526

Ixodes scapularis

Deer tick

Isca\ISCW018522

Orthologs in non-Arthropod Metazoa (EOG679T0V)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Caenorhabditis elegans

Nematode

Cele\sel-2

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_009382gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_004484gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_025957gn

Danio rerio

Zebrafish

Drer\nbea

Danio rerio

Zebrafish

Drer\BX530409.1

Danio rerio

Zebrafish

Drer\si:dkey-257n17.3

Xenopus tropicalis

Western clawed frog

Xtro\ENSXETG00000026062

Xenopus tropicalis

Western clawed frog

Xtro\nbea

Xenopus tropicalis

Western clawed frog

Xtro\ENSXETG00000026016

Gallus gallus

Domestic chicken

Ggal\LRBA

Gallus gallus

Domestic chicken

Ggal\NBEA

Mus musculus

House mouse

Mmus\Lrba

Mus musculus

House mouse

Mmus\Nbea

Rattus norvegicus

Norway rat

Rnor\Lrba

Rattus norvegicus

Norway rat

Rnor\ENSRNOG00000014747

Homo sapiens

Human

Hsap\ENSG00000198589

Homo sapiens

Human

Hsap\NBEA

Human Orthologs (2)

Gene

OMIM

HGNC

Hsap\ENSG00000198589

Hsap\NBEA

AAA Orthologs (0) based on analysis using Dmel annotation version 4.3

No orthologs identified

Stocks & Reagents

Stocks Listed in FlyBase ( 20 )

Bloomington

12980

y¹ P{SUPor-P}rg^KG02343

23371

w¹¹¹⁸ Mi{ET1}rg^MB01845

v107056

v26090

w¹¹¹⁸; P{GD10753}v26090/CyO

More stocks available...

Genomic Clones ( 3 )

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 53 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

IP18551

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

DRSC - Results from RNAi screens.

•

FBgn0086911

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

44531

Antibody Information

polyclonal

(FlyBase Genome Annotators, 2006)

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for identity of: Akap550 CG6775

(Zhao, 1999.11)

Source for database merge of

Source for merge of: rg Akap550

(Venkatesh et al., 2001)

Source for merge of: rg CG15466

Additional comments

Release 2 annotation CG15466 (which was eliminated in release 3 of the genome annotation) has been merged with CG6775 (which corresponds to rg) in release 5.1 of the genome annotation.

(FlyBase Genome Annotators, 2006)

Not allelic to N or rst.

(FlyBase Genome Annotators, 2002-2003)

may be allelic to lzl: lozenge-like

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Annotation CG15466 was eliminated in release 3 of the genome annotation, October 2002.

Cloning and sequencing of rg cDNA, the differential expression of the protein during embryogenesis and the mapping of the binding site are studied.

(Renfranz and Benzer, 1989)

The interaction of rap and rg with S is synergistic and dosage sensitive.

(Venkatesh et al., 1995)

lzl may be allelic to rg.

(Oliver et al., 1990)

The abnormal rg phenotype is due to a developmental defect: late occurring defects. Adult eye is rough with variable facet shape, variable number of rhabdomeres per ommatidium, eye disc pattern appears normal.

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

RefSeq (Proteins)

•

DDBJ / EMBL / GenBank

•

Entrez Gene - A searchable database of RefSeq genes.

•

44531

UniProtKB/Swiss-Prot

•

Q9W4E2

UniProtKB/TrEMBL

•

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

WD40-repeat-containing domain (IPR017986)

BEACH domain (IPR000409)

WD40 repeat (IPR001680)

Concanavalin A-like lectin/glucanases superfamily (IPR008985)

Domain of unknown function DUF1088 (IPR010508)

WD40/YVTN repeat-like-containing domain (IPR015943)

PH-BEACH domain (IPR023362)

Armadillo-type fold (IPR016024)

Concanavalin A-like lectin/glucanase, subgroup (IPR013320)

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

•

GH23814

Linkouts

BioGRID - A database of protein and genetic interactions

•

69112

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0086911

DRSC - Results from RNAi screens.

•

FBgn0086911

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0086911

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0086911

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

44531

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

•

/hjmuller/rugose1.htm

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

44531

modMine - Data generated by the modENCODE project.

•

FBgn0086911

SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores.

•

Q9W4E2

Synonyms & Secondary IDs ( 12 )

Reported As

Symbol Synonym

AKAP

(Schwaerzel et al., 2007)

Akap550

(Anholt et al., 2003, Han et al., 1997, Wang et al., 2004)

CG6775

(Keleman et al., 2009.8.5)

CG15466

(Luschnig, 2003.12.17, )

DAKAP550

(Wang et al., 2000, Venkatesh et al., 2001, Jackson and Berg, 2000, Han, 1997.5.12, Han et al., 1997, Guan et al., 2011)

fas

(Schreiber et al., 2002, Negre et al., 2006, Wech and Nagel, 2005, Guan et al., 2011, Kankel et al., 2007, Dworkin et al., 2009, Manak et al., 2006, Volders et al., 2012, Wasbrough et al., 2010)

Name Synonym

A kinase anchor protein 550

Drosophila A kinase anchor protein

facetious