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Gene Dmel\Mst77F

General Information
Symbol	Dmel\Mst77F	Species	D. melanogaster
Name	Male-specific transcript 77F	Annotation symbol	CG3354
Feature type	protein_coding_gene	FlyBase ID	FBgn0086915
Gene Model Status	Current	Stock availability	5 publicly available
Genomic Location
Chromosome (arm)	3L	Recombination map	3-[46]
Cytogenetic map	77E8-77F	Sequence location	3L:20,810,001..20,811,150 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Male-specific transcript 77F is referred to in FlyBase by the symbol Dmel\Mst77F (CG3354, FBgn0086915). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: sperm chromatin condensation. 6 alleles are reported. The phenotype of these alleles is annotated with: spermatid. It has one annotated transcript and one annotated polypeptide. Protein features are: High mobility group, superfamily. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of no expression detected. Peak expression observed at stages throughout the pupal period, in adult male stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval fat body, adult testis. Comments on Affy2 ProbeSet: ProbeSet 1628256_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Mst77F. Gene sequence location is 3L:20810001..20811150.
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	ms(3)nc3 Fails to complement certain βTub mutant alleles for male fertility although mapping to another site. Male fertility of homozygotes not determined. ms(3)nc3/+ males fertile.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2011_10	Clones MIP31849
FB2012_01	Sequence features BKN25084 HFA11871 BKN46462
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 77E8-77F   Limits computationally determined from genome sequence between P{EP}trblEP3519 and P{EP}fngEP3082&P{lacW}skdL7062
Experimentally Determined Cytological Location
Cytogenetic map Notes References 77F-77F     (Kalderon and Rubin, 1988)
Experimentally Determined Recombination Data
Location	3-47.0 +/- 0.1 (Fuller, 1986) 3-[46]
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Mst77F for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Mst77F-RA FBtr0078270  NM_079464   1088   215
Additional Transcript Data & Comments
Reported size (kB)	1.4 (northern blot) (Kalderon and Rubin, 1988)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Mst77F-PA   FBpp0077928   24.5   215   10.46     NP_524188   AAF51647
Additional Polypeptide Data & Comments
Reported size (kDa)	215 (aa) (Kalderon and Rubin, 1988)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • High mobility group, superfamily (IPR009071)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014296 AAF51647   AI945818   AI946428   AI946506   AY089414   BE975027   BE975070   BE978948   BF485704   BF485768   BF488799   BF489009   BF489083   BF495264   BF504286   BF505804   BG634184   BG634528   BI592207   BT004840 AAO45196   BT128734   BT132683   CO181914   CO302257   EC211668   X16962 CAA34836
UniProtKB/Swiss-Prot	P16909
UniProtKB/TrEMBL	Q8T402
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
northern blot Stage Tissue/Position (including subcellular localization) Reference pupal stage & adult stage     (Kalderon and Rubin, 1988)
Additional Descriptive Data
	Mst77F is expressed at high levels in pupae and adults. (Kalderon and Rubin, 1988)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0086915    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0086915 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of no expression detected. Peak expression observed at stages throughout the pupal period, in adult male stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0086915 expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    2 embryo 04-06hr    2 embryo 06-08hr    11 embryo 08-10hr    4 embryo 10-12hr    1 embryo 12-14hr    8 embryo 14-16hr    7 embryo 16-18hr    5 embryo 18-20hr    8 embryo 20-22hr    7 embryo 22-24hr    13 larva L1    13 larva L2    9 larva L3 12hr old    46 larva L3 puffstage 1-2    1746 larva L3 puffstage 3-6    3809 larva L3 puffstage 7-9    5626 white prepupae new    5312 white prepupae 12hr    10085 white prepupae 24hr    13504 pupae 2d postWPP    16179 pupae 3d postWPP    17709 pupae 4d postWPP    11737 adult male 01day    24865 adult male 05day    27709 adult male 30day    20899 adult female 01day    15 adult female 05day    13 adult female 30day    9 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    2 embryo 04-06hr    2 embryo 06-08hr    11 embryo 08-10hr    4 embryo 10-12hr    1 embryo 12-14hr    8 embryo 14-16hr    7 embryo 16-18hr    5 embryo 18-20hr    8 embryo 20-22hr    7 embryo 22-24hr    13 larva L1    13 larva L2    9 larva L3 12hr old    46 larva L3 puffstage 1-2  (1746) larva L3 puffstage 3-6  (3809) larva L3 puffstage 7-9  (5626) white prepupae new  (5312) white prepupae 12hr  (10085) white prepupae 24hr  (13504) pupae 2d postWPP  (16179) pupae 3d postWPP  (17709) pupae 4d postWPP  (11737) adult male 01day  (24865) adult male 05day  (27709) adult male 30day  (20899) adult female 01day    15 adult female 05day    13 adult female 30day    9 Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    2 embryo 04-06hr    2 embryo 06-08hr    11 embryo 08-10hr    4 embryo 10-12hr    1 embryo 12-14hr    8 embryo 14-16hr    7 embryo 16-18hr    5 embryo 18-20hr    8 embryo 20-22hr    7 embryo 22-24hr    13 larva L1    13 larva L2    9 larva L3 12hr old    46 larva L3 puffstage 1-2  (1746) larva L3 puffstage 3-6  (3809) larva L3 puffstage 7-9  (5626) white prepupae new  (5312) white prepupae 12hr  (10085) white prepupae 24hr  (13504) pupae 2d postWPP  (16179) pupae 3d postWPP  (17709) pupae 4d postWPP  (11737) adult male 01day  (24865) adult male 05day  (27709) adult male 30day  (20899) adult female 01day    15 adult female 05day    13 adult female 30day    9 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    2 embryo 04-06hr    2 embryo 06-08hr    11 embryo 08-10hr    4 embryo 10-12hr    1 embryo 12-14hr    8 embryo 14-16hr    7 embryo 16-18hr    5 embryo 18-20hr    8 embryo 20-22hr    7 embryo 22-24hr    13 larva L1    13 larva L2    9 larva L3 12hr old    46 larva L3 puffstage 1-2    1746 larva L3 puffstage 3-6    3809 larva L3 puffstage 7-9    5626 white prepupae new    5312 white prepupae 12hr  10085 white prepupae 24hr  (13504) pupae 2d postWPP  (16179) pupae 3d postWPP  (17709) pupae 4d postWPP  (11737) adult male 01day  (24865) adult male 05day  (27709) adult male 30day  (20899) adult female 01day    15 adult female 05day    13 adult female 30day    9 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    2 embryo 04-06hr    2 embryo 06-08hr    11 embryo 08-10hr    4 embryo 10-12hr    1 embryo 12-14hr    8 embryo 14-16hr    7 embryo 16-18hr    5 embryo 18-20hr    8 embryo 20-22hr    7 embryo 22-24hr    13 larva L1    13 larva L2    9 larva L3 12hr old    46 larva L3 puffstage 1-2    1746 larva L3 puffstage 3-6    3809 larva L3 puffstage 7-9    5626 white prepupae new    5312 white prepupae 12hr    10085 white prepupae 24hr    13504 pupae 2d postWPP    16179 pupae 3d postWPP    17709 pupae 4d postWPP    11737 adult male 01day    24865 adult male 05day    27709 adult male 30day    20899 adult female 01day    15 adult female 05day    13 adult female 30day    9 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0086915 expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    2 embryo 04-06hr    2 embryo 06-08hr    11 embryo 08-10hr    4 embryo 10-12hr    1 embryo 12-14hr    8 embryo 14-16hr    7 embryo 16-18hr    5 embryo 18-20hr    8 embryo 20-22hr    7 embryo 22-24hr    13 larva L1    13 larva L2    9 larva L3 12hr old    46 larva L3 puffstage 1-2    1746 larva L3 puffstage 3-6    3809 larva L3 puffstage 7-9    5626 white prepupae new    5312 white prepupae 12hr    10085 white prepupae 24hr    13504 pupae 2d postWPP    16179 pupae 3d postWPP    17709 pupae 4d postWPP    11737 adult male 01day    24865 adult male 05day    27709 adult male 30day    20899 adult female 01day    15 adult female 05day    13 adult female 30day    9 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    2 embryo 04-06hr    2 embryo 06-08hr    11 embryo 08-10hr    4 embryo 10-12hr    1 embryo 12-14hr    8 embryo 14-16hr    7 embryo 16-18hr    5 embryo 18-20hr    8 embryo 20-22hr    7 embryo 22-24hr    13 larva L1    13 larva L2    9 larva L3 12hr old    46 larva L3 puffstage 1-2  (1746) larva L3 puffstage 3-6  (3809) larva L3 puffstage 7-9  (5626) white prepupae new  (5312) white prepupae 12hr  (10085) white prepupae 24hr  (13504) pupae 2d postWPP  (16179) pupae 3d postWPP  (17709) pupae 4d postWPP  (11737) adult male 01day  (24865) adult male 05day  (27709) adult male 30day  (20899) adult female 01day    15 adult female 05day    13 adult female 30day    9 Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    2 embryo 04-06hr    2 embryo 06-08hr    11 embryo 08-10hr    4 embryo 10-12hr    1 embryo 12-14hr    8 embryo 14-16hr    7 embryo 16-18hr    5 embryo 18-20hr    8 embryo 20-22hr    7 embryo 22-24hr    13 larva L1    13 larva L2    9 larva L3 12hr old    46 larva L3 puffstage 1-2  1746 larva L3 puffstage 3-6  (3809) larva L3 puffstage 7-9  (5626) white prepupae new  (5312) white prepupae 12hr  (10085) white prepupae 24hr  (13504) pupae 2d postWPP  (16179) pupae 3d postWPP  (17709) pupae 4d postWPP  (11737) adult male 01day  (24865) adult male 05day  (27709) adult male 30day  (20899) adult female 01day    15 adult female 05day    13 adult female 30day    9 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    2 embryo 04-06hr    2 embryo 06-08hr    11 embryo 08-10hr    4 embryo 10-12hr    1 embryo 12-14hr    8 embryo 14-16hr    7 embryo 16-18hr    5 embryo 18-20hr    8 embryo 20-22hr    7 embryo 22-24hr    13 larva L1    13 larva L2    9 larva L3 12hr old    46 larva L3 puffstage 1-2    1746 larva L3 puffstage 3-6    3809 larva L3 puffstage 7-9    5626 white prepupae new    5312 white prepupae 12hr  10085 white prepupae 24hr  13504 pupae 2d postWPP  16179 pupae 3d postWPP  17709 pupae 4d postWPP  11737 adult male 01day  (24865) adult male 05day  (27709) adult male 30day  (20899) adult female 01day    15 adult female 05day    13 adult female 30day    9 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    2 embryo 04-06hr    2 embryo 06-08hr    11 embryo 08-10hr    4 embryo 10-12hr    1 embryo 12-14hr    8 embryo 14-16hr    7 embryo 16-18hr    5 embryo 18-20hr    8 embryo 20-22hr    7 embryo 22-24hr    13 larva L1    13 larva L2    9 larva L3 12hr old    46 larva L3 puffstage 1-2    1746 larva L3 puffstage 3-6    3809 larva L3 puffstage 7-9    5626 white prepupae new    5312 white prepupae 12hr    10085 white prepupae 24hr    13504 pupae 2d postWPP    16179 pupae 3d postWPP    17709 pupae 4d postWPP    11737 adult male 01day    24865 adult male 05day    27709 adult male 30day    20899 adult female 01day    15 adult female 05day    13 adult female 30day    9 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0086915    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0086915 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval fat body, adult testis. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0086915 expression level Tissue   Expression Level Larval Central Nervous System    2.275 Larval Midgut no informative data Larval Hindgut    5.1 Larval Malpighian Tubules no informative data Larval Fat Body    1191.7 Larval Salivary Gland    6.6 Larval Trachea no informative data Larval Carcass no informative data Adult Head no informative data Adult Eye    5.075 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion    9.2 Adult Crop    9.7 Adult Midgut    4.9 Adult Hindgut    6.5 Adult Malpighian Tubules    6.1 Adult Fat Body    8.4 Adult Salivary Gland    2.4 Adult Heart    4.275 Adult VirginFemale Spermatheca    1.7 Adult InseminatedFemale Spermatheca    4.5 Adult Ovary    3.2 Adult Testis    3857.4 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    2.275 Larval Midgut no informative data Larval Hindgut    5.1 Larval Malpighian Tubules no informative data Larval Fat Body  (1191.7) Larval Salivary Gland    6.6 Larval Trachea no informative data Larval Carcass no informative data Adult Head no informative data Adult Eye    5.075 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion    9.2 Adult Crop    9.7 Adult Midgut    4.9 Adult Hindgut    6.5 Adult Malpighian Tubules    6.1 Adult Fat Body    8.4 Adult Salivary Gland    2.4 Adult Heart    4.275 Adult VirginFemale Spermatheca    1.7 Adult InseminatedFemale Spermatheca    4.5 Adult Ovary    3.2 Adult Testis  (3857.4) Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    2.275 Larval Midgut no informative data Larval Hindgut    5.1 Larval Malpighian Tubules no informative data Larval Fat Body  (1191.7) Larval Salivary Gland    6.6 Larval Trachea no informative data Larval Carcass no informative data Adult Head no informative data Adult Eye    5.075 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion    9.2 Adult Crop    9.7 Adult Midgut    4.9 Adult Hindgut    6.5 Adult Malpighian Tubules    6.1 Adult Fat Body    8.4 Adult Salivary Gland    2.4 Adult Heart    4.275 Adult VirginFemale Spermatheca    1.7 Adult InseminatedFemale Spermatheca    4.5 Adult Ovary    3.2 Adult Testis  (3857.4) Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    2.275 Larval Midgut no informative data Larval Hindgut    5.1 Larval Malpighian Tubules no informative data Larval Fat Body    1191.7 Larval Salivary Gland    6.6 Larval Trachea no informative data Larval Carcass no informative data Adult Head no informative data Adult Eye    5.075 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion    9.2 Adult Crop    9.7 Adult Midgut    4.9 Adult Hindgut    6.5 Adult Malpighian Tubules    6.1 Adult Fat Body    8.4 Adult Salivary Gland    2.4 Adult Heart    4.275 Adult VirginFemale Spermatheca    1.7 Adult InseminatedFemale Spermatheca    4.5 Adult Ovary    3.2 Adult Testis    3857.4 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0086915 expression level Tissue   Expression Level Larval Central Nervous System    2.275 Larval Midgut no informative data Larval Hindgut    5.1 Larval Malpighian Tubules no informative data Larval Fat Body    1191.7 Larval Salivary Gland    6.6 Larval Trachea no informative data Larval Carcass no informative data Adult Head no informative data Adult Eye    5.075 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion    9.2 Adult Crop    9.7 Adult Midgut    4.9 Adult Hindgut    6.5 Adult Malpighian Tubules    6.1 Adult Fat Body    8.4 Adult Salivary Gland    2.4 Adult Heart    4.275 Adult VirginFemale Spermatheca    1.7 Adult InseminatedFemale Spermatheca    4.5 Adult Ovary    3.2 Adult Testis    3857.4 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    2.275 Larval Midgut no informative data Larval Hindgut    5.1 Larval Malpighian Tubules no informative data Larval Fat Body  (1191.7) Larval Salivary Gland    6.6 Larval Trachea no informative data Larval Carcass no informative data Adult Head no informative data Adult Eye    5.075 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion    9.2 Adult Crop    9.7 Adult Midgut    4.9 Adult Hindgut    6.5 Adult Malpighian Tubules    6.1 Adult Fat Body    8.4 Adult Salivary Gland    2.4 Adult Heart    4.275 Adult VirginFemale Spermatheca    1.7 Adult InseminatedFemale Spermatheca    4.5 Adult Ovary    3.2 Adult Testis  (3857.4) Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    2.275 Larval Midgut no informative data Larval Hindgut    5.1 Larval Malpighian Tubules no informative data Larval Fat Body  1191.7 Larval Salivary Gland    6.6 Larval Trachea no informative data Larval Carcass no informative data Adult Head no informative data Adult Eye    5.075 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion    9.2 Adult Crop    9.7 Adult Midgut    4.9 Adult Hindgut    6.5 Adult Malpighian Tubules    6.1 Adult Fat Body    8.4 Adult Salivary Gland    2.4 Adult Heart    4.275 Adult VirginFemale Spermatheca    1.7 Adult InseminatedFemale Spermatheca    4.5 Adult Ovary    3.2 Adult Testis  (3857.4) Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    2.275 Larval Midgut no informative data Larval Hindgut    5.1 Larval Malpighian Tubules no informative data Larval Fat Body    1191.7 Larval Salivary Gland    6.6 Larval Trachea no informative data Larval Carcass no informative data Adult Head no informative data Adult Eye    5.075 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion    9.2 Adult Crop    9.7 Adult Midgut    4.9 Adult Hindgut    6.5 Adult Malpighian Tubules    6.1 Adult Fat Body    8.4 Adult Salivary Gland    2.4 Adult Heart    4.275 Adult VirginFemale Spermatheca    1.7 Adult InseminatedFemale Spermatheca    4.5 Adult Ovary    3.2 Adult Testis    3857.4 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut no informative data Larval Hindgut     Larval Malpighian Tubules no informative data Larval Fat Body     Larval Salivary Gland     Larval Trachea no informative data Larval Carcass no informative data Adult Head no informative data Adult Eye     Adult Brain no informative data Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland no informative data Adult Carcass no informative data FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    no informative data   NA  Eye    5.075   NA  Brain    no informative data   2.275  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    9.2   NA  Crop    9.7   no informative data  Midgut    4.9   5.1  Hindgut    6.5   no informative data  Malpighian Tubules    6.1   1191.7  Fat Body    8.4   6.6  Salivary Gland    2.4   NA  Heart    4.275   no informative data  Trachea    NA   NA  VirginFemale Spermatheca    1.7   NA  InseminatedFemale Spermatheca    4.5   NA  Ovary    3.2   NA  Testis    3857.4   NA  Male Accessory Gland    no informative data   no informative data  Carcass    no informative data modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0086915
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele semi-lethal | pupal stage, with Scer\GAL4pnr-MD237 Mst77FGD10945 viable Mst77Fc06969 viable (with Df(3L)ri-79c) Mst77Fc06969 Sterility Allele fertile Mst77Fc06969 fertile (with Df(3L)ri-79c) Mst77Fc06969 male sterile (with Df(3L)ri-79c) Mst77F1 male sterile (with Mst77F1) Mst77Fc06969 male sterile (with Mst77Fc06969) Mst77F1 Phenotype manifest in Allele chromatin & late spermatid (with Df(3L)ri-79c) Mst77F1 chromatin & late spermatid (with Mst77F1) Mst77Fc06969 chromatin & late spermatid (with Mst77Fc06969) Mst77F1 nucleus & late spermatid (with Df(3L)ri-79c) Mst77F1 nucleus & late spermatid (with Mst77F1) Mst77Fc06969 nucleus & late spermatid (with Mst77Fc06969) Mst77F1 nucleus & spermatozoon Mst77F1 spermatid (with Df(3L)ri-79c) Mst77F1
Classical Alleles ( 2 )
For All Classical Alleles Show
Allele of Mst77F Class Mutagen Stocks Known lesion Mst77Fc06969 2 Yes Mst77F1 ethyl methanesulfonate 0 Yes
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Mst77F Class Mutagen Stocks Known lesion Mst77FGD10945 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Mst77FGL00317 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Mst77FHMS00633 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Mst77FT:Avic\GFP-EGFP in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD10945} NA P{TRiP.GL00317} NA P{TRiP.HMS00633} NA characterization construct P{Mst77F-eGFP} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{PB}Pka-R1c06969 NA
Gene Ontology: Function, Process & Cellular Component ( 3 unique terms )
Terms Based on Experimental Evidence ( 3 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
sperm chromatin condensation inferred from mutant phenotype (Raja and Renkawitz-Pohl, 2005)
Cellular Component
CV term Evidence References
flagellum inferred from direct assay (Raja and Renkawitz-Pohl, 2005, Rathke et al., 2010) nucleus inferred from direct assay (Raja and Renkawitz-Pohl, 2005, Rathke et al., 2010)
Terms Based on Predictions or Assertions ( 0 terms )
Sequence Ontology: Class of Gene
	gene   nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Mst77F allele Gene References ms(3)nc31 βTub85D (Fuller, 1986)
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0086915
Orthologs
Genome-wide drosophilid orthologs
Curated drosophilid orthologs	Dsim\Mst77F
Linkouts	OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0239937 FBgn0239598 FBgn0183894 FBgn0177053 FBgn0249087 FBgn0085494 FBgn0105550 FBgn0087243
Stocks & Reagents
Stocks Listed in FlyBase ( 5 )
Bloomington	35397 y1 sc* v1; P{TRiP.GL00317}attP2 17792 w1118; PBac{PB}Pka-R1c06969 Mst77Fc06969 32849 y1 sc* v1; P{TRiP.HMS00633}attP2/TM3, Sb1
Harvard	- PBac{PB}Pka-R1c06969
VDRC	v41923 w1118 P{GD10945}v41923
Genomic Clones ( 1 )
	BACR22I16 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 19 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	AT04151 AT23593 RH09844
Other clones	MIP31849
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT05605 AT08196 AT19650 AT19814 AT24442 AT24693 AT24783 AT30311 AT30762
Other clones	59365 bs37g10 bs38c06 bs84g07 EC15796 EK183745
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0086915 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 40304 48308
Antibody Information
	polyclonal (Rathke et al., 2010)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: Mst77F ms(3)nc3 (Raja and Renkawitz-Pohl, 2005)
Additional comments	18 Y-linked genes that are related to the Mst77F gene (which is located on 3L) have been identified. Evidence suggests that these multiple copies originated from a single duplication from the autosome to the Y chromosome, followed by duplications inside the Y. Ten of these Y-linked genes encode defective proteins (Mst77Y-3Ψ, Mst77Y-5Ψ, Mst77Y-6Ψ, Mst77Y-10Ψ, Mst77Y-11Ψ, Mst77Y-14Ψ, Mst77Y-15Ψ, Mst77Y-16Ψ, Mst77Y-17Ψ and Mst77Y-18Ψ) and the other eight are potentially functional (Mst77Y-1, Mst77Y-2, Mst77Y-4, Mst77Y-7, Mst77Y-8, Mst77Y-9, Mst77Y-12 and Mst77Y-13). The eight potentially functional genes produce approximately 20% of the functional Mst77F-like mRNA and evolved under purifying selection. (Krsticevic et al., 2010)
Other Comments
	The Mst77F polypeptide is the first candidate for a specialized DNA binding protein involved in compaction of Drosophila sperm chromatin. (Russell and Kaiser, 1993) Located within an intron of Pka-R1, transcribed in the opposite orientation to Pka-R1. (Kalderon and Rubin, 1988)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079464 RefSeq (Proteins) • NP_524188 DDBJ / EMBL / GenBank • AAF51647 AAF51647.1 AAO45196 AI945818 AI946428 AI946506 BI592207 BT004840 CAA34836 X16962 Entrez Gene - A searchable database of RefSeq genes. • 40304 UniProtKB/Swiss-Prot • P16909 UniProtKB/TrEMBL • Q8T402
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • High mobility group, superfamily (IPR009071)
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0086915 DRSC - Results from RNAi screens. • FBgn0086915 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0086915 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0086915 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 40304 48308 modMine - Data generated by the modENCODE project. • FBgn0086915 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0239937 FBgn0239598 FBgn0183894 FBgn0177053 FBgn0249087 FBgn0085494 FBgn0105550 FBgn0087243
Synonyms & Secondary IDs ( 9 )
Reported As
Symbol Synonym	18c (Kalderon and Rubin, 1988, ) CG3354 (Ni et al., 2010.12.1, Ni et al., 2011.7.19) ms(3)nc3 (Rathke et al., 2007) Mst77F (Raja and Renkawitz-Pohl, 2005, Rathke et al., 2007, Osoegawa et al., 2007, Raja and Renkawitz-Pohl, 2006, Rathke et al., 2007, Vardanyan et al., 2008, Krsticevic et al., 2010, Rathke et al., 2010, Dubruille et al., 2010) mst77F (Balakirev and Ayala, 2003, Russell and Kaiser, 1993, Gatti and Pimpinelli, 1992) nc3 (Fuller, 1986, ) R1-18c
Name Synonym	Male-specific transcript 77F   male sterile (3) nc3
Secondary FlyBase IDs
	FBgn0000093 FBgn0002839
References ( 22 )
Generate a list of	All references relating to this gene ( 22 )
List References by type	Research paper  ( 10 ) Supplementary material  ( 1 ) Review  ( 2 ) Erratum  ( 1 ) Personal communication to FlyBase  ( 4 ) Abstract  ( 1 ) Computer file  ( 1 ) Book  ( 1 )
Recent research papers ( 3 )
	Dubruille et al., 2010, Curr. Biol. 20(23): 2090--2099 Specialization of a Drosophila Capping Protein Essential for the Protection of Sperm Telomeres. [FBrf0212516] Krsticevic et al., 2010, Genetics 184(1): 295--307 Functional copies of the Mst77F gene on the Y chromosome of Drosophila melanogaster. [FBrf0209663] Rathke et al., 2010, Europ. J. Cell Biol. 89(4): 326--338 Distinct functions of Mst77F and protamines in nuclear shaping and chromatin condensation during Drosophila spermiogenesis. [FBrf0210142] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...