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General Information
Symbol
Dmel\5-HT2A
Species
D. melanogaster
Name
5-hydroxytryptamine (serotonin) receptor 2A
Annotation Symbol
CG1056
Feature Type
FlyBase ID
FBgn0087012
Gene Model Status
Stock Availability
Gene Summary
5-hydroxytryptamine (serotonin) receptor 2A (5-HT2A) encodes a 5-hydroxytryptamine (serotonin) GPCR that belongs to the Class A GPCR family. They bind and transmit the signal from the neurotransmitter 5-HT (serotonin). The family can be subdivided into several subclasses based on pharmacology, signal transduction and structure. There are 5 genes encoding serotonin receptors in the Drosophila melanogaster genome: 5-HT1A, 5-HT1B, 5-HT2A, 5-HT2B, and 5-HT7. [Date last reviewed: 2019-09-26] (FlyBase Gene Snapshot)
Also Known As

5-HT2, 5-HT2Dro, 5-HT2, 5HT2Dro, 5-HT2Dro

Key Links
Genomic Location
Cytogenetic map
Sequence location
Recombination map
3-47.1
RefSeq locus
NT_033777 REGION:4612870..4633304
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (14 terms)
Molecular Function (5 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
inferred from sequence or structural similarity
inferred from biological aspect of ancestor with PANTHER:PTN000664111
(assigned by GO_Central )
inferred from sequence or structural similarity
traceable author statement
non-traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN000664111
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN004683911
(assigned by GO_Central )
Biological Process (5 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from mutant phenotype
inferred from direct assay
inferred from mutant phenotype
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000664111
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000664111
(assigned by GO_Central )
traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN004683911
(assigned by GO_Central )
inferred from sequence or structural similarity
Cellular Component (4 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
located_in plasma membrane
inferred from direct assay
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
is_active_in dendrite
inferred from biological aspect of ancestor with PANTHER:PTN000664111
(assigned by GO_Central )
inferred from sequence or structural similarity
inferred from biological aspect of ancestor with PANTHER:PTN000664111
(assigned by GO_Central )
located_in plasma membrane
inferred from sequence or structural similarity
Protein Family (UniProt)
-
Summaries
Gene Snapshot
5-hydroxytryptamine (serotonin) receptor 2A (5-HT2A) encodes a 5-hydroxytryptamine (serotonin) GPCR that belongs to the Class A GPCR family. They bind and transmit the signal from the neurotransmitter 5-HT (serotonin). The family can be subdivided into several subclasses based on pharmacology, signal transduction and structure. There are 5 genes encoding serotonin receptors in the Drosophila melanogaster genome: 5-HT1A, 5-HT1B, 5-HT2A, 5-HT2B, and 5-HT7. [Date last reviewed: 2019-09-26]
Gene Group (FlyBase)
5-HYDROXYTRYPTAMINE (SEROTONIN) GPCRs -
5-hydroxytryptamine (serotonin) GPCRs belong to the Class A GPCR family. They bind and transmit the signal from the neurotransmitter 5-HT (serotonin). The family can be subdivided into seven subclasses: 5-HT1-7 based on pharmacology, signal transduction and structure. (Adapted from PMID:18571247).
Summary (Interactive Fly)

seven-pass transmembrane protein - coordinates germband extension movements

Gene Model and Products
Number of Transcripts
5
Number of Unique Polypeptides
5

Please see the JBrowse view of Dmel\5-HT2A for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure New Section
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q8IPN2)

If you don't see the viewer to the right, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.53

Gene model reviewed during 5.49

Gene model reviewed during 5.55

Low-frequency RNA-Seq exon junction(s) not annotated.

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0078854
4355
868
FBtr0078855
3722
930
FBtr0300474
3178
638
FBtr0339395
3541
916
FBtr0339397
3683
917
Additional Transcript Data and Comments
Reported size (kB)

3.9 (compiled cDNA)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0078494
95.1
868
8.90
FBpp0078495
101.6
930
8.82
FBpp0289701
70.1
638
6.93
FBpp0308487
100.0
916
8.70
FBpp0308489
100.1
917
8.70
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\5-HT2A using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
dorsal ectoderm anlage

Comment: anlage in statu nascendi

ventral ectoderm anlage

Comment: anlage in statu nascendi

Additional Descriptive Data

5-HT2A is expressed in seven stripes in the blastoderm embryo. The first two and last two stripes are stronger than the middle three. Expression remains detectable through gastrulation with expression weaker in the 4th and 5th stripes. Expression fades during germ band extension.

5-HT2 transcripts are present in embryos, larvae, and adults with a peak in 3-6hr embryos. They are first detected by in situ hybridization in cellular blastoderm embryos in even pair-rule stripes which persist through germ band extension. They are later seen in a pair of cells per neuromere in the CNS starting at stage 13.

Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in plasma membrane
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\5-HT2A in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 16 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 11 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of 5-HT2A
Transgenic constructs containing regulatory region of 5-HT2A
Aberrations (Deficiencies and Duplications) ( 9 )
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
10 of 15
Yes
Yes
2  
7 of 15
No
Yes
7 of 15
No
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
10 of 15
Yes
Yes
7 of 15
No
Yes
6 of 15
No
Yes
Rattus norvegicus (Norway rat) (3)
7 of 13
Yes
Yes
6 of 13
No
Yes
6 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (2)
5 of 12
Yes
Yes
2 of 12
No
Yes
Danio rerio (Zebrafish) (5)
8 of 15
Yes
Yes
7 of 15
No
Yes
7 of 15
No
Yes
6 of 15
No
Yes
5 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (2)
6 of 15
Yes
Yes
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Other Organism Orthologs (via OrthoDB)
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (8)
2 of 10
2 of 10
2 of 10
2 of 10
1 of 10
1 of 10
1 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Allele
Disease
Evidence
References
Potential Models Based on Orthology ( 3 )
Human Ortholog
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Allele
Disease
Interaction
References
Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
esyN Network Diagram
Interactions Browser
Summary of Genetic Interactions
esyN Network Diagram
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
Pathways
Signaling Pathways (FlyBase)
Metabolic Pathways
FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
External Data
Linkouts
KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
Genomic Location and Detailed Mapping Data
Chromosome (arm)
3R
Recombination map
3-47.1
Cytogenetic map
Sequence location
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
82C4-82D5
Left limit from inclusion within Df(3R)110 (FBrf0104943) Right limit from non-inclusion within Df(3R)6-7 (FBrf0104943)
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
82C-82D
(determined by in situ hybridisation)
Experimentally Determined Recombination Data
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (20)
Genomic Clones (16)
 

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (28)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced
BDGP DGC clones
Other clones
Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
RNAi and Array Information
Linkouts
DRSC - Results frm RNAi screens
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
Antibody Information
Laboratory Generated Antibodies
 
Commercially Available Antibodies
 
Other Information
Relationship to Other Genes
Source for database identify of

Source for identity of: 5-HT2A 5-HT2

Source for database merge of

Source for merge of: 5-HT2 l(3)82CDb

Additional comments
Other Comments

Shows particularly robust cycling of transcription in adult heads, as assessed by expression analysis using high density oligonucleotide arrays with probe generated during three 12-point time course experiments over the course of 6 days.

Serotinin, acting through the 5-HT2 gene product, is necessary for proper gastrulation.

Deletion of 5-HT2 is associated with a specific, double-line cuticular phenotype.

Peaks of 5-HT2 expression and serotonin synthesis coincide precisely with the onset of convergent extension of the ectoderm.

Mutants do not extend the germband properly, and ectodermal movements become asynchronous with the morphogenetic movements in the endoderm and mesoderm. Adherens junctions in the ectoderm show altered subcellular distribution at the onset of the asynchronicity.

Identified in a screen for genes encoding G-protein coupled receptors.

Characterisation of 5-HT2 reveals it is expressed in the central nervous system during larval and adult stages and the receptor is expressed at the blastoderm stage in a pattern similar to that of ftz.

This serotonin receptor displays a sequence, gene organisation and pharmacology typical of the mammalian serotonin 5-HT2 receptor type (5-HT2B subtype) acting on phospholipase C.

Origin and Etymology
Discoverer
Etymology
Identification
External Crossreferences and Linkouts ( 64 )
Sequence Crossreferences
NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
Other crossreferences
AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
InterPro - A database of protein families, domains and functional sites
KEGG Genes - Molecular building blocks of life in the genomic space.
MARRVEL_MODEL - MARRVEL (model organism gene)
modMine - A data warehouse for the modENCODE project
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
DRSC - Results frm RNAi screens
FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
FlyMine - An integrated database for Drosophila genomics
Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
Synonyms and Secondary IDs (30)
Reported As
Symbol Synonym
5HT-R82A
5HT-dro1
Dm5-HT2A
Name Synonyms
5-HT2Dro receptor
5-hydroxytryptamine (serotonin) receptor 2A
Serotonin receptor 2
lethal (3) 82CDb
serotonin receptor 2a
Secondary FlyBase IDs
  • FBgn0013743
  • FBgn0025495
Datasets (1)
Study focus (0)
Experimental Role
Project
Project Type
Title
Study result (1)
Result
Result Type
Title
Clustering analysis of hemocytes from wasp-infested third instar larvae
References (107)