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Gene Dmel\AGO2

General Information
Symbol	Dmel\AGO2	Species	D. melanogaster
Name	Argonaute 2	Annotation symbol	CG7439
Feature type	protein_coding_gene	FlyBase ID	FBgn0087035
Gene Model Status	Current	Stock availability	10 publicly available
Also Known As	Ago-2, dAgo2
Genomic Location
Chromosome (arm)	3L	Recombination map	3-[42]
Cytogenetic map	71C3-71E5	Sequence location	3L:15,547,207..15,554,142 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Argonaute 2 is referred to in FlyBase by the symbol Dmel\AGO2 (CG7439, FBgn0087035). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding; endoribonuclease activity; siRNA binding. There is experimental evidence for 14 unique biological process terms, many of which group under: biological regulation; gene silencing; cellular component organization or biogenesis; localization; programmed cell death; anatomical structure formation involved in morphogenesis; immune system process; pole cell development; embryonic pattern specification; gland morphogenesis; RNA interference; cell death. 39 alleles are reported. The phenotypes of these alleles are annotated with: pole cell; condensed chromosome; pole bud. It has 3 annotated transcripts and 3 annotated polypeptides. Protein features are: Argonaute/Dicer protein, PAZ; Domain of unknown function DUF1785; Ribonuclease H-like; Stem cell self-renewal protein Piwi. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderately high expression. Peak expression observed at stages throughout embryogenesis, during late larval stages, at stages throughout the pupal period, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult spermathecae, adult male accessory gland, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult ovary, adult testis. Comments on Affy2 ProbeSet: ProbeSet 1636068_a_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of AGO2. Gene sequence location is 3L:15547207..15554142.
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	References Knowles-Barley, 2011.8.24, BrainTrap expression curation. Jovel and Schneemann, 2011, Virology 419(1): 43--53 Sanders and Smith, 2011, PLoS ONE 6(8): e24151
FB2012_01	References Khurana et al., 2010, PLoS Genet. 6(12): e1001246 Maiti et al., 2011, Chemistry 17(5): 1519--1528 Sequence features HFA10847 HFA09999 BKN26052
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 71C3-71E5   Left limit from inclusion within Df(3L)BK10 (FBrf0057258) Right limit from inclusion within Df(3L)BK10 (FBrf0057258)
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location	3-[42]
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\AGO2 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	DGC clone GM07030 appears problematic: incomplete CDS
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) AGO2-RB FBtr0075559  NM_140518   4056   1214 AGO2-RC FBtr0075560  NM_168626   4114   1217 AGO2-RD FBtr0308124     3757   694
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein AGO2-PB   FBpp0075312   136.9   1214   10.05     NP_648775   AAF49619 AGO2-PC   FBpp0075313   137.2   1217   10.00     NP_730054   AAF49620 AGO2-PD   FBpp0300446   79.7   694   9.68
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Argonaute/Dicer protein, PAZ (IPR003100) Stem cell self-renewal protein Piwi (IPR003165) Ribonuclease H-like (IPR012337) Domain of unknown function DUF1785 (IPR014811)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA696644   AA697782   AA803111   AA816282   AE014296 AAF49619   AE014296 AAF49620   AF222007   AI534851   AI945716   AW941283   AY094751 AAM11104   BG639471   BG640026   BI164635   BI165027   BI166132   BI166465   BI169081   BI215715   BI232765   BI238513   BI239989   BI242470   BI358052   BI371109   BI373545   BI577439   BI579458   BI580236   BP550128   BT003546 AAO39550   BT099682   CK658543   CL527754   CO155937   CO177081   CO177453   CO178413   CO184440   CO189178   CO193452   CO194968   CO263155   CO276183   CO277451   CO279088   CO282449   CO282758   CO285582   CO290024   CO290041   CO290680   CO300257   CO301903   CO304377   CO311752   CO313515   CO331131   CO338396   CO345391   DQ228766 ABB54719   DQ228767 ABB54720   DQ228768 ABB54721   DQ228769 ABB54722   DQ228770 ABB54723   DQ228771 ABB54724   DQ228772 ABB54725   DQ228773 ABB54726   EC069703   EC083202   EC086726   EC196356   EC196362   EC202729   EC210943   EC211640   EC221512   EC223117   EC225307   EC230701   EC231219   EC234082   EC234338   EC235668   EC241670   EC242052   EC244529   EC248565   EC254030   EC263933   EC268008   EV585249   EV595667   EV598839   GH753496   GH759337   GH760982   GH771787   GH774988   GH777300   GH778487   GH781387   GH781992   GH782060   GH783265   GH784569   GH785339   GH785489   GH786143   GH787468   GH787669   GH788812   GH789673   GH790358   GH791888   GH793950   GH794612   GH795639   GH796349   GH799316   GH799793   GH800164   GH801289   GH801955   GH804067   GH804643   GH805437   GH805483   GH806960   GH807128   GH807823   GH807944   GH808974   GH810228   GH810568   GH812383   GH813884   GH814569   GH815978   GH818877   GH819655   GH820304   GH821252   GH821462   GH827915   GH830699   GH832241   GH833372   GH833398   GH834214   GH838739   GH839795   GH841081   GH841510   GH842065   GH843161   GH846676   GH847647   GH848501   GH851566   GH852435   GH854640   GH855603   GH857556   GH857655   GH858727   GH859554   GH860105   GH861717   GH866261   GH866960   GH868565   GH869309   GH872301   GH872450   GH873628   GH873959   GH874744   GH874964   GH876626   GH878056   GH880864   GH881008   GH882167   GH885830   GH886855   GH888973   GH891007   GH891071   GH892940   GH893520   GH894153   GH895312   GH898991   GH899412   GH899653   GH900781   GH901499   GH901962   GH904111   GH905112   GH905738   GH906370   GH906453   GH909622   GH909971   GH909985   GH912421   GH913580   GH915307   GH918408   GH918680   GH919848   GH921691   GH923630   GH925209   GH926564   GH926864   GH928139   GH929443   GH930489   GH934263   GH936390   GH936413   GH939151   GH939969
UniProtKB/Swiss-Prot	Q9VUQ5
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0087035    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0087035 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderately high expression. Peak expression observed at stages throughout embryogenesis, during late larval stages, at stages throughout the pupal period, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0087035 expression level Developmental Stage   Expression Level embryo 00-02hr    3723 embryo 02-04hr    5542 embryo 04-06hr    6528 embryo 06-08hr    5475 embryo 08-10hr    5620 embryo 10-12hr    4726 embryo 12-14hr    5280 embryo 14-16hr    4899 embryo 16-18hr    5139 embryo 18-20hr    4356 embryo 20-22hr    3866 embryo 22-24hr    4424 larva L1    2488 larva L2    2454 larva L3 12hr old    2622 larva L3 puffstage 1-2    4883 larva L3 puffstage 3-6    6678 larva L3 puffstage 7-9    7088 white prepupae new    7089 white prepupae 12hr    6250 white prepupae 24hr    5934 pupae 2d postWPP    4942 pupae 3d postWPP    4282 pupae 4d postWPP    2607 adult male 01day    3931 adult male 05day    3940 adult male 30day    3967 adult female 01day    4315 adult female 05day    5004 adult female 30day    4548 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (3723) embryo 02-04hr  (5542) embryo 04-06hr  (6528) embryo 06-08hr  (5475) embryo 08-10hr  (5620) embryo 10-12hr  (4726) embryo 12-14hr  (5280) embryo 14-16hr  (4899) embryo 16-18hr  (5139) embryo 18-20hr  (4356) embryo 20-22hr  (3866) embryo 22-24hr  (4424) larva L1  (2488) larva L2  (2454) larva L3 12hr old  (2622) larva L3 puffstage 1-2  (4883) larva L3 puffstage 3-6  (6678) larva L3 puffstage 7-9  (7088) white prepupae new  (7089) white prepupae 12hr  (6250) white prepupae 24hr  (5934) pupae 2d postWPP  (4942) pupae 3d postWPP  (4282) pupae 4d postWPP  (2607) adult male 01day  (3931) adult male 05day  (3940) adult male 30day  (3967) adult female 01day  (4315) adult female 05day  (5004) adult female 30day  (4548) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (3723) embryo 02-04hr  (5542) embryo 04-06hr  (6528) embryo 06-08hr  (5475) embryo 08-10hr  (5620) embryo 10-12hr  (4726) embryo 12-14hr  (5280) embryo 14-16hr  (4899) embryo 16-18hr  (5139) embryo 18-20hr  (4356) embryo 20-22hr  (3866) embryo 22-24hr  (4424) larva L1  (2488) larva L2  (2454) larva L3 12hr old  (2622) larva L3 puffstage 1-2  (4883) larva L3 puffstage 3-6  (6678) larva L3 puffstage 7-9  (7088) white prepupae new  (7089) white prepupae 12hr  (6250) white prepupae 24hr  (5934) pupae 2d postWPP  (4942) pupae 3d postWPP  (4282) pupae 4d postWPP  (2607) adult male 01day  (3931) adult male 05day  (3940) adult male 30day  (3967) adult female 01day  (4315) adult female 05day  (5004) adult female 30day  (4548) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    3723 embryo 02-04hr    5542 embryo 04-06hr    6528 embryo 06-08hr    5475 embryo 08-10hr    5620 embryo 10-12hr    4726 embryo 12-14hr    5280 embryo 14-16hr    4899 embryo 16-18hr    5139 embryo 18-20hr    4356 embryo 20-22hr    3866 embryo 22-24hr    4424 larva L1    2488 larva L2    2454 larva L3 12hr old    2622 larva L3 puffstage 1-2    4883 larva L3 puffstage 3-6    6678 larva L3 puffstage 7-9    7088 white prepupae new    7089 white prepupae 12hr    6250 white prepupae 24hr    5934 pupae 2d postWPP    4942 pupae 3d postWPP    4282 pupae 4d postWPP    2607 adult male 01day    3931 adult male 05day    3940 adult male 30day    3967 adult female 01day    4315 adult female 05day    5004 adult female 30day    4548 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    3723 embryo 02-04hr    5542 embryo 04-06hr    6528 embryo 06-08hr    5475 embryo 08-10hr    5620 embryo 10-12hr    4726 embryo 12-14hr    5280 embryo 14-16hr    4899 embryo 16-18hr    5139 embryo 18-20hr    4356 embryo 20-22hr    3866 embryo 22-24hr    4424 larva L1    2488 larva L2    2454 larva L3 12hr old    2622 larva L3 puffstage 1-2    4883 larva L3 puffstage 3-6    6678 larva L3 puffstage 7-9    7088 white prepupae new    7089 white prepupae 12hr    6250 white prepupae 24hr    5934 pupae 2d postWPP    4942 pupae 3d postWPP    4282 pupae 4d postWPP    2607 adult male 01day    3931 adult male 05day    3940 adult male 30day    3967 adult female 01day    4315 adult female 05day    5004 adult female 30day    4548 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0087035 expression level Developmental Stage   Expression Level embryo 00-02hr    3723 embryo 02-04hr    5542 embryo 04-06hr    6528 embryo 06-08hr    5475 embryo 08-10hr    5620 embryo 10-12hr    4726 embryo 12-14hr    5280 embryo 14-16hr    4899 embryo 16-18hr    5139 embryo 18-20hr    4356 embryo 20-22hr    3866 embryo 22-24hr    4424 larva L1    2488 larva L2    2454 larva L3 12hr old    2622 larva L3 puffstage 1-2    4883 larva L3 puffstage 3-6    6678 larva L3 puffstage 7-9    7088 white prepupae new    7089 white prepupae 12hr    6250 white prepupae 24hr    5934 pupae 2d postWPP    4942 pupae 3d postWPP    4282 pupae 4d postWPP    2607 adult male 01day    3931 adult male 05day    3940 adult male 30day    3967 adult female 01day    4315 adult female 05day    5004 adult female 30day    4548 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (3723) embryo 02-04hr  (5542) embryo 04-06hr  (6528) embryo 06-08hr  (5475) embryo 08-10hr  (5620) embryo 10-12hr  (4726) embryo 12-14hr  (5280) embryo 14-16hr  (4899) embryo 16-18hr  (5139) embryo 18-20hr  (4356) embryo 20-22hr  (3866) embryo 22-24hr  (4424) larva L1  (2488) larva L2  (2454) larva L3 12hr old  (2622) larva L3 puffstage 1-2  (4883) larva L3 puffstage 3-6  (6678) larva L3 puffstage 7-9  (7088) white prepupae new  (7089) white prepupae 12hr  (6250) white prepupae 24hr  (5934) pupae 2d postWPP  (4942) pupae 3d postWPP  (4282) pupae 4d postWPP  (2607) adult male 01day  (3931) adult male 05day  (3940) adult male 30day  (3967) adult female 01day  (4315) adult female 05day  (5004) adult female 30day  (4548) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (3723) embryo 02-04hr  (5542) embryo 04-06hr  (6528) embryo 06-08hr  (5475) embryo 08-10hr  (5620) embryo 10-12hr  (4726) embryo 12-14hr  (5280) embryo 14-16hr  (4899) embryo 16-18hr  (5139) embryo 18-20hr  (4356) embryo 20-22hr  (3866) embryo 22-24hr  (4424) larva L1  (2488) larva L2  2454 larva L3 12hr old  (2622) larva L3 puffstage 1-2  (4883) larva L3 puffstage 3-6  (6678) larva L3 puffstage 7-9  (7088) white prepupae new  (7089) white prepupae 12hr  (6250) white prepupae 24hr  (5934) pupae 2d postWPP  (4942) pupae 3d postWPP  (4282) pupae 4d postWPP  (2607) adult male 01day  (3931) adult male 05day  (3940) adult male 30day  (3967) adult female 01day  (4315) adult female 05day  (5004) adult female 30day  (4548) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    3723 embryo 02-04hr    5542 embryo 04-06hr    6528 embryo 06-08hr    5475 embryo 08-10hr    5620 embryo 10-12hr    4726 embryo 12-14hr    5280 embryo 14-16hr    4899 embryo 16-18hr    5139 embryo 18-20hr    4356 embryo 20-22hr    3866 embryo 22-24hr    4424 larva L1    2488 larva L2    2454 larva L3 12hr old    2622 larva L3 puffstage 1-2    4883 larva L3 puffstage 3-6    6678 larva L3 puffstage 7-9    7088 white prepupae new    7089 white prepupae 12hr    6250 white prepupae 24hr    5934 pupae 2d postWPP    4942 pupae 3d postWPP    4282 pupae 4d postWPP    2607 adult male 01day    3931 adult male 05day    3940 adult male 30day    3967 adult female 01day    4315 adult female 05day    5004 adult female 30day    4548 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    3723 embryo 02-04hr    5542 embryo 04-06hr    6528 embryo 06-08hr    5475 embryo 08-10hr    5620 embryo 10-12hr    4726 embryo 12-14hr    5280 embryo 14-16hr    4899 embryo 16-18hr    5139 embryo 18-20hr    4356 embryo 20-22hr    3866 embryo 22-24hr    4424 larva L1    2488 larva L2    2454 larva L3 12hr old    2622 larva L3 puffstage 1-2    4883 larva L3 puffstage 3-6    6678 larva L3 puffstage 7-9    7088 white prepupae new    7089 white prepupae 12hr    6250 white prepupae 24hr    5934 pupae 2d postWPP    4942 pupae 3d postWPP    4282 pupae 4d postWPP    2607 adult male 01day    3931 adult male 05day    3940 adult male 30day    3967 adult female 01day    4315 adult female 05day    5004 adult female 30day    4548 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0087035    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0087035 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult spermathecae, adult male accessory gland, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult ovary, adult testis. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0087035 expression level Tissue   Expression Level Larval Central Nervous System    764.575 Larval Midgut    744.5 Larval Hindgut    821.6 Larval Malpighian Tubules    966.5 Larval Fat Body    971.6 Larval Salivary Gland    1804.6 Larval Trachea    1379.625 Larval Carcass    979.725 Adult Head    671.2 Adult Eye    628.575 Adult Brain    622.9 Adult Thoracic-Abdominal Ganglion    548.5 Adult Crop    1009.5 Adult Midgut    759.3 Adult Hindgut    818.2 Adult Malpighian Tubules    1067 Adult Fat Body    951.2 Adult Salivary Gland    1173 Adult Heart    876.025 Adult VirginFemale Spermatheca    771.7 Adult InseminatedFemale Spermatheca    790.3 Adult Ovary    447.3 Adult Testis    272.5 Adult Male Accessory Gland    864.3 Adult Carcass    760.4 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (764.575) Larval Midgut  (744.5) Larval Hindgut  (821.6) Larval Malpighian Tubules  (966.5) Larval Fat Body  (971.6) Larval Salivary Gland  (1804.6) Larval Trachea  (1379.625) Larval Carcass  (979.725) Adult Head  (671.2) Adult Eye  (628.575) Adult Brain  (622.9) Adult Thoracic-Abdominal Ganglion  (548.5) Adult Crop  (1009.5) Adult Midgut  (759.3) Adult Hindgut  (818.2) Adult Malpighian Tubules  (1067) Adult Fat Body  (951.2) Adult Salivary Gland  (1173) Adult Heart  (876.025) Adult VirginFemale Spermatheca  (771.7) Adult InseminatedFemale Spermatheca  (790.3) Adult Ovary    447.3 Adult Testis    272.5 Adult Male Accessory Gland  (864.3) Adult Carcass  (760.4) Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    764.575 Larval Midgut    744.5 Larval Hindgut    821.6 Larval Malpighian Tubules    966.5 Larval Fat Body    971.6 Larval Salivary Gland  (1804.6) Larval Trachea  (1379.625) Larval Carcass    979.725 Adult Head    671.2 Adult Eye    628.575 Adult Brain    622.9 Adult Thoracic-Abdominal Ganglion    548.5 Adult Crop  1009.5 Adult Midgut    759.3 Adult Hindgut    818.2 Adult Malpighian Tubules  1067 Adult Fat Body    951.2 Adult Salivary Gland  (1173) Adult Heart    876.025 Adult VirginFemale Spermatheca    771.7 Adult InseminatedFemale Spermatheca    790.3 Adult Ovary    447.3 Adult Testis    272.5 Adult Male Accessory Gland    864.3 Adult Carcass    760.4 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    764.575 Larval Midgut    744.5 Larval Hindgut    821.6 Larval Malpighian Tubules    966.5 Larval Fat Body    971.6 Larval Salivary Gland    1804.6 Larval Trachea    1379.625 Larval Carcass    979.725 Adult Head    671.2 Adult Eye    628.575 Adult Brain    622.9 Adult Thoracic-Abdominal Ganglion    548.5 Adult Crop    1009.5 Adult Midgut    759.3 Adult Hindgut    818.2 Adult Malpighian Tubules    1067 Adult Fat Body    951.2 Adult Salivary Gland    1173 Adult Heart    876.025 Adult VirginFemale Spermatheca    771.7 Adult InseminatedFemale Spermatheca    790.3 Adult Ovary    447.3 Adult Testis    272.5 Adult Male Accessory Gland    864.3 Adult Carcass    760.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0087035 expression level Tissue   Expression Level Larval Central Nervous System    764.575 Larval Midgut    744.5 Larval Hindgut    821.6 Larval Malpighian Tubules    966.5 Larval Fat Body    971.6 Larval Salivary Gland    1804.6 Larval Trachea    1379.625 Larval Carcass    979.725 Adult Head    671.2 Adult Eye    628.575 Adult Brain    622.9 Adult Thoracic-Abdominal Ganglion    548.5 Adult Crop    1009.5 Adult Midgut    759.3 Adult Hindgut    818.2 Adult Malpighian Tubules    1067 Adult Fat Body    951.2 Adult Salivary Gland    1173 Adult Heart    876.025 Adult VirginFemale Spermatheca    771.7 Adult InseminatedFemale Spermatheca    790.3 Adult Ovary    447.3 Adult Testis    272.5 Adult Male Accessory Gland    864.3 Adult Carcass    760.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  764.575 Larval Midgut  744.5 Larval Hindgut  (821.6) Larval Malpighian Tubules  (966.5) Larval Fat Body  (971.6) Larval Salivary Gland  (1804.6) Larval Trachea  (1379.625) Larval Carcass  (979.725) Adult Head  671.2 Adult Eye  628.575 Adult Brain  622.9 Adult Thoracic-Abdominal Ganglion  548.5 Adult Crop  (1009.5) Adult Midgut  759.3 Adult Hindgut  (818.2) Adult Malpighian Tubules  (1067) Adult Fat Body  (951.2) Adult Salivary Gland  (1173) Adult Heart  (876.025) Adult VirginFemale Spermatheca  771.7 Adult InseminatedFemale Spermatheca  790.3 Adult Ovary    447.3 Adult Testis    272.5 Adult Male Accessory Gland  (864.3) Adult Carcass  760.4 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    764.575 Larval Midgut    744.5 Larval Hindgut    821.6 Larval Malpighian Tubules    966.5 Larval Fat Body    971.6 Larval Salivary Gland  (1804.6) Larval Trachea  1379.625 Larval Carcass    979.725 Adult Head    671.2 Adult Eye    628.575 Adult Brain    622.9 Adult Thoracic-Abdominal Ganglion    548.5 Adult Crop  1009.5 Adult Midgut    759.3 Adult Hindgut    818.2 Adult Malpighian Tubules  1067 Adult Fat Body    951.2 Adult Salivary Gland  1173 Adult Heart    876.025 Adult VirginFemale Spermatheca    771.7 Adult InseminatedFemale Spermatheca    790.3 Adult Ovary    447.3 Adult Testis    272.5 Adult Male Accessory Gland    864.3 Adult Carcass    760.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    764.575 Larval Midgut    744.5 Larval Hindgut    821.6 Larval Malpighian Tubules    966.5 Larval Fat Body    971.6 Larval Salivary Gland    1804.6 Larval Trachea    1379.625 Larval Carcass    979.725 Adult Head    671.2 Adult Eye    628.575 Adult Brain    622.9 Adult Thoracic-Abdominal Ganglion    548.5 Adult Crop    1009.5 Adult Midgut    759.3 Adult Hindgut    818.2 Adult Malpighian Tubules    1067 Adult Fat Body    951.2 Adult Salivary Gland    1173 Adult Heart    876.025 Adult VirginFemale Spermatheca    771.7 Adult InseminatedFemale Spermatheca    790.3 Adult Ovary    447.3 Adult Testis    272.5 Adult Male Accessory Gland    864.3 Adult Carcass    760.4 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    671.2   NA  Eye    628.575   NA  Brain    622.9   764.575  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    548.5   NA  Crop    1009.5   744.5  Midgut    759.3   821.6  Hindgut    818.2   966.5  Malpighian Tubules    1067   971.6  Fat Body    951.2   1804.6  Salivary Gland    1173   NA  Heart    876.025   1379.625  Trachea    NA   NA  VirginFemale Spermatheca    771.7   NA  InseminatedFemale Spermatheca    790.3   NA  Ovary    447.3   NA  Testis    272.5   NA  Male Accessory Gland    864.3   979.725  Carcass    760.4 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_23
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0087035
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele semi-lethal | embryonic stage AGO251B AGO2414 viable AGO251B AGO2321 AGO2414 AGO2454 AGO2EY04479 AGO2V966M viable, with Scer\GAL4pnr-MD237 AGO2GD17090 Sterility Allele fertile AGO2414 AGO2EY04479 AGO2V966M Other Phenotypes Allele decreased cell number AGO2414 decreased cell number | embryonic stage AGO251B immune response defective AGO2414 immune response defective | conditional AGO2414 increased cell death AGO2dsRNA.cRa mitotic cell cycle defective AGO2414 suppressor of variegation | dominant AGO2414 wild-type AGO2414 Phenotype manifest in Allele condensed chromosome AGO2414 nucleus | embryonic stage 4 AGO2414 pole bud AGO2414 pole cell AGO2414 AGO251B
Classical Alleles ( 13 )
For All Classical Alleles Show
Allele of AGO2 Class Mutagen Stocks Known lesion AGO2321 amorphic allele - molecular evidence Delta2-3 1 Yes AGO2414 loss of function allele, hypomorphic allele - molecular evidence Delta2-3 1 Yes AGO2454 Delta2-3 1 Yes AGO2EY04479 P-element activity 1 -- AGO2NP3072 1 -- AGO2V966M ethyl methanesulfonate 1 Yes AGO251B Delta2-3 0 Yes AGO2CA07002 P-element activity 0 -- AGO2CPTI000743 piggyBac transposase 0 Yes AGO2CPTI002343 piggyBac transposase 0 Yes AGO2EP3417 P-element activity 0 Yes AGO2NP4402 0 -- AGO2S1008 natural population 0 Yes
Alleles Carried on Transgenic Constructs ( 26 )
For All Alleles Carried on Transgenic Constructs Show
Allele of AGO2 Class Mutagen Stocks Known lesion AGO2GD17090 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes AGO2HMS00108 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes AGO2KK106052 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes AGO2T:Zzzz\FLAG,T:Ivir\HA1 in vitro construct - coding region fusion 1 Yes AGO2+t10.1 in vitro construct - genomic fragment 0 Yes AGO2Act5C.T:Disc\RFP in vitro construct - coding region fusion in vitro construct - regulatory fusion 0 Yes AGO2dsRNA.1-1000 in vitro construct - RNAi 0 Yes AGO2dsRNA.2500-3435 in vitro construct - RNAi 0 Yes AGO2dsRNA.C in vitro construct - RNAi 0 Yes AGO2dsRNA.cCa in vitro construct - RNAi 0 Yes AGO2dsRNA.cDa in vitro construct - RNAi 0 Yes AGO2dsRNA.cDb in vitro construct - RNAi 0 Yes AGO2dsRNA.cFa in vitro construct - RNAi 0 Yes AGO2dsRNA.cIa in vitro construct - RNAi 0 Yes AGO2dsRNA.cJa in vitro construct - RNAi 0 Yes AGO2dsRNA.cOa in vitro construct - RNAi 0 Yes AGO2dsRNA.cOb in vitro construct - RNAi 0 Yes AGO2dsRNA.cRa in vitro construct - RNAi 0 Yes AGO2dsRNA.si.1695-1717 in vitro construct - RNAi 0 Yes AGO2dsRNA.si.3293-3315 in vitro construct - RNAi 0 Yes AGO2GM07030.Scer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes AGO2MtnA.T:Zzzz\His6 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes AGO2MtnA.T:Zzzz\TAP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes AGO2Scer\UAS.cMa in vitro construct - regulatory fusion 0 Yes AGO2T:Uuuu\T7 in vitro construct - coding region fusion 0 Yes AGO2αTub67C.PM in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD17090} NA P{KK106052} NA P{TRiP.HMS00108} NA P{UAS-AGO2.GM07030.HA} NA P{UAS-AGO2.M} NA characterization construct P{AGO2+t10.1} NA P{FLAG.HA.AGO2} NA P{tub-AGO2.mat} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}AGO2EP3417 NA P{EPgy2}AGO2EY04479 NA P{Mae-UAS.6.11}B2036B2036 NA insertion of enhancer trap binary system P{GawB}AGO2NP3072 No P{GawB}AGO2NP4402 No miscellaneous insertions P{?EP}AGO2321 NA P{PTT-GA}AGO2CA07002 NA PBac{544.SVS-1}AGO2CPTI002343 NA PBac{602.P.SVS-1}AGO2CPTI000743 NA
Gene Ontology: Function, Process & Cellular Component ( 23 unique terms )
Terms Based on Experimental Evidence ( 20 terms )
Molecular Function
CV term Evidence References
endoribonuclease activity inferred from direct assay (Rand et al., 2005) protein binding inferred from physical interaction with AGO1 (Meyer et al., 2006) inferred from physical interaction with Dcr-1 (Hammond et al., 2001) inferred from physical interaction with Fmr1 (Ishizuka et al., 2002) siRNA binding inferred from direct assay (Rand et al., 2005, Lingel et al., 2003, Kawamura et al., 2008)
Biological Process
CV term Evidence References
autophagic cell death inferred from expression pattern (Gorski et al., 2003) cellularization inferred from mutant phenotype (Deshpande et al., 2005) defense response to virus inferred from mutant phenotype (Wang et al., 2006, Mueller et al., 2010) dsRNA transport inferred from mutant phenotype (Saleh et al., 2006) NOT gene silencing by miRNA inferred from mutant phenotype (Rehwinkel et al., 2005) heterochromatin organization involved in chromatin silencing inferred from mutant phenotype (Fagegaltier et al., 2009) negative regulation of viral genome replication inferred from mutant phenotype (Sabin et al., 2009) pole cell formation inferred from mutant phenotype (Deshpande et al., 2005) production of siRNA involved in RNA interference inferred from direct assay (Hammond et al., 2001) inferred from mutant phenotype (Okamura et al., 2008) RNA interference inferred from direct assay (Matranga et al., 2005, Rand et al., 2005) inferred from mutant phenotype (Ishizuka et al., 2002, Dorner et al., 2006, Rehwinkel et al., 2005, Meyer et al., 2006) salivary gland cell autophagic cell death inferred from expression pattern (Gorski et al., 2003) segment polarity determination inferred from genetic interaction with AGO1 (Meyer et al., 2006) siRNA loading onto RISC involved in RNA interference inferred from direct assay (Kim et al., 2007) inferred from mutant phenotype (Okamura et al., 2004) syncytial nuclear migration inferred from mutant phenotype (Deshpande et al., 2005)
Cellular Component
CV term Evidence References
cytoplasm inferred from direct assay (Reich et al., 2009) RISC-loading complex inferred from direct assay (Kim et al., 2007) RNA-induced silencing complex inferred from direct assay (Hammond et al., 2001, Kim et al., 2007, Liu et al., 2009)
Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function
CV term Evidence References
translation initiation factor activity inferred from sequence or structural similarity with UniProtKB:Q9UL18 (FlyBase, 1992-)
Biological Process
CV term Evidence References
RNA interference non-traceable author statement (Skipper, 2004) traceable author statement (Cerutti, 2003) targeting of mRNA for destruction involved in RNA interference traceable author statement (Williams and Rubin, 2002) translational initiation inferred from sequence or structural similarity with UniProtKB:Q9UL18 (FlyBase, 1992-)
Cellular Component
CV term Evidence References
RNA-induced silencing complex traceable author statement (Ishizuka et al., 2002, Nelson et al., 2003, Caudy et al., 2002)
Sequence Ontology: Class of Gene
	gene   nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions AGO2 allele Gene References unspecified AGO1 (Meyer et al., 2006)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 65002 DroID - A comprehensive database of gene and protein interactions. • FBgn0087035 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 39683
Orthologs
Genome-wide drosophilid orthologs
Curated drosophilid orthologs	Dana\AGO2 (Meyer et al., 2006) Dmoj\AGO2 (Meyer et al., 2006) Dpse\GA20353 (Meyer et al., 2006) Dsan\AGO2 (Obbard et al., 2005.10.3) Dsim\AGO2 (Obbard et al., 2005.10.3, Obbard et al., 2005.10.3, Obbard et al., 2005.10.3, Obbard et al., 2005.10.3, Obbard et al., 2005.10.3, Obbard et al., 2005.10.3, Obbard et al., 2005.10.3, Obbard et al., 2005.10.3) Dtei\AGO2 (Obbard et al., 2005.10.3) Dvir\AGO2 (Meyer et al., 2006) Dyak\AGO2 (Obbard et al., 2005.10.3, Obbard et al., 2005.10.3, Obbard et al., 2005.10.3, Obbard et al., 2005.10.3, Obbard et al., 2005.10.3, Obbard et al., 2005.10.3, Obbard et al., 2005.10.3, Obbard et al., 2005.10.3)
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AGAP011537-PA Anopheles gambiae (mosquito) XP_395048.3 Apis mellifera (honey bee) XP_001944852.1 Acyrthosiphon pisum (pea aphid) BGIBMGA010406-PA Bombyx mori (silk worm) CPIJ009898 Culex pipens (mosquito) XP_001607164.1 Nasonia vitripennis (wasp) PHUM002582-PA Pediculus humanus (human body louse) NP_001107828.1 Tribolium castaneum (red flour beetle) 20630 Thalassiosira pseudonana (marine diatom) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB16541 NV12825 NV12824 LH23016 FBgn0085112 FBgn0212615 FBgn0195702 FBgn0084451 FBgn0180394 FBgn0245029 FBgn0162465 FBgn0135876 FBgn0122217 FBgn0108142 FBgn0087097 CPIJ014791 CPIJ009898 BGIBMGA010406 ACYPI008293 GB15464 AGAP011537 AAEL017251 PHUM004130 GLEAN_11525 GLEAN_13762
Stocks & Reagents
Stocks Listed in FlyBase ( 10 )
Bloomington	33242 w1118; P{FLAG.HA.AGO2}2 36511 w1118; AGO2321/TM3, Sb1 16608 y1 w67c23; P{EPgy2}AGO2EY04479 34799 y1 sc* v1; P{TRiP.HMS00108}attP2 32062 yd2 w1118 P{ey-FLP.N}2; AGO2V966M 36512 w1118; AGO2454/TM3, Sb1 Ser1
Kyoto	109027 w*; AGO2414 104364 w*; P{GawB}AGO2NP3072
VDRC	v100356 P{KK106052}VIE-260B v49473 w1118; P{GD17090}v49473
Genomic Clones ( 2 )
	BACR07L07 BACR09C21 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 209 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GM07030 RE04347 RE36670
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GM08058 HL03146 LD01139 LP15542 RE04827 RE06162 RE06592 RE10056 RE22310 RE28889 RE34995 RE36628 RE39801 RE44719 RE57933 RE60864 RE71115 RE73489 RE74390
Other clones	5452 17652 18832 45136 45534 53108 57323 59014 73105 74093 102477 107550 214251.48 525753 571358 bs16a06 CK01.64C.G5 CK01.71B.D2 CK01.87A.C7 CK01.91B.F9 CK01.93C.C3 CK01.93C.H3 CK01.104C.A4 Dmel_dig_006551_0657_0834 Dmel_neb_009678_0921_1344 Dmel_neb_012686_0626_3249 EC28095 EC35918 EC39533 EK043338 EK052421 EK053532 EK056645 EK072910 EK072930 EK074747 EK087634 EK119905 EK124629 EK133518 EK160519 EK161145 EK169512 EK177757 EK182541 EK226059 EK233152 EK244527 EK298442 EN07051 EN16714 EP21952 FGM105D11 RP003003814 RP003006000 RP003008818 RP003009473 RP003010481 RP003011588 RP003011956 RP003013926 RP003027773 RP003029148 RP003035245 RP003038405 RP003044412 RP003048385 RP003051154 RP003054737 RP003064043 RP003065395 RP003066106 RP003073988 RP003078828 RP003090237 RP003093344 RP003094186 RP003095798 RP003103201 RP003108954 RP003111409 RP003113545 RP003114827 RP003116700 RP003123339 RP003134158 RP003146363 RP003150705 RP003152308 RP003157686 RP003161105 RP003163366 RP003164343 RP003164781 RP003179007 RP003192925 RP003207066 RP003208809 RP003210417 RP003211275 RP003221206 RP003227510 RP003230547 RP003234855 RP003241993 RP003243150 RP003243663 RP003245633 RP003257694 RP003268401 RP003269011 RP003283770 RP003291419 RP003295472 RP003305201 RP003310484 RP003312861 RP003315812 RP003318643 RP003319229 RP003322062 RP003328327 RP003331027 RP003337757 RP003342928 RP003346633 RP003346922 RP003360575 RP003361045 RP003375453 RP003376274 RP003383693 RP003390813 RP003399121 RP003409617 RP003411009 RP003414852 RP003415789 RP003417023 RP003417427 RP003420386 RP003423142 RP003429664 RP003440407 RP003442707 RP003443022 RP003447453 RP003453626 RP003464183 RP003472055 RP003477027 RP003507141 RP003507757 RP003508848 RP003511059 RP003515572 RP003518234 RP003534270 RP003546014 RP003546068 RP003547927 RP003550496 RP003555492 RP003565654 RP003571931 RP003573595 RP003575030 RP003575175 RP003583945 RP003585646 RP003608270 RP003611764 RP003614150 RP003618002 RP003618227 RP003620584 RP003624138 RP003628083 RP003628462 RP003633395 RP003633498 RP003642000 RP003644457 RP003648514 RP003660661 SD01108 SD10540
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0087035 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 39683 45982
Antibody Information
	polyclonal (Rehwinkel et al., 2006)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: AGO2 CG7439 (Hammond et al., 2001)
Source for database merge of	Source for merge of: AGO2 CG13452 (FlyBase Genome Annotators, 2002-2003)
Additional comments	"dop" is not allelic to "AGO2". (Hain et al., 2010) In contrast to what is stated in FBrf0194224, the "drop out" complementation group is not allelic to "AGO2". Recently isolated mRNA null mutations of the AGO2 locus fully complement dop mutant alleles. The molecular lesions of dop mutant alleles were reported in FBrf0194224 to represent in frame deletions of glutamine rich repeats (GRR) in the amino-terminus of AGO2. Analysis of 24 different D.melanogaster haplotypes show that the amino-terminal domain of AGO2 is highly variable and that the alteration of the amino-terminal GRR pattern observed in the dop[46] allele is not unique. The GRR haplotype of dop[46] is not the cause of the developmental phenotype of embryos derived from homozygous mothers. A D.melanogaster strain with the identical GRR haplotype does not exhibit gross developmental defects. (Muller, 2010.6.29) FlyBase curator comment: In contrast to what is stated in FBrf0194224, the "drop out" complementation group is not allelic to "AGO2" (see FBrf0211203 for more details). (Meyer et al., 2006) Annotations CG7439 and CG13452 merged as CG7439 in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003)
Other Comments
	The N-terminal glutamine rich repeat (GRR) domain of the long isoform of AGO2 appears to be subject to rapid evolution, with extensive variation in GRR copy number being seen among D. melanogaster strains isolated from natural and laboratory populations. GRR variation does not cause striking defects in embryonic development and assembly of the RNA induced silencing complex is unimpaired in embryos when GRR copy number is altered. The AGO2 locus also produces a putative short isoform that does not contain the N-terminal GRR containing domain. (Hain et al., 2010) Many miRNA* species, unlike their partner mature miRNAs, are preferentially incorporated into the AGO2 complex and are 3' modified. The miRNA strand selection of AGO2 is very sensitive to the base-pairing status of the 9th and 10th position of the miRNA duplex. (Okamura et al., 2009) AGO2 binds endogenous short interfering RNAs (esiRNAs) derived from retrotransposons and esiRNAs arising from stem-loop structures. (Kawamura et al., 2008) Production of mature siRNAs from endogenous long hairpin RNA genes is a hybrid mechanism that combines canonical RNA interference factors (Dcr-2, Hen1 and AGO2) and a canonical microRNA factor loqs. (Okamura et al., 2008) Double-stranded miRNAs and siRNAs participate in a common sorting step that partitions them into AGO1- or AGO2-containing effector complexes. (Förstemann et al., 2007) The Dcr-2-r2d2 heterodimer acts as a gatekeeper for the assembly of AGO2 complexes, promoting the incorporation of siRNAs and disfavoring miRNAs as loading substrates for AGO2. A separate mechanism acts in parallel to favor miRNA/miRNA* duplexes and exclude siRNAs from assembly into AGO1 complexes. (Tomari et al., 2007) dsRNA made from templates generated with primers directed against this gene are used to infer a role for this gene in the miRNA pathway. (Behm-Ansmant et al., 2006) AGO2 is in the top 3% of fastest-evolving Drosophila genes. (Obbard et al., 2006) AGO2 is not required for repeat-associated small interfering RNA (rasiRNA) production. (Vagin et al., 2006) In vitro assays show that the AGO2 protein directly receives the double-stranded siRNA from the RISC complex machinery and cleaves the siRNA passenger strand, releasing the single-stranded guide. (Matranga et al., 2005) AGO2 protein associated with guide siRNA is able to cleave passenger siRNA. (Miyoshi et al., 2005) AGO2 protein binds both strands of siRNA and cleaves the anti-guide strand to produce active RISC. (Rand et al., 2005) dsRNA has been made from templates generated with primers directed against this gene. (Rehwinkel et al., 2005) AGO1 and AGO2 are not redundant. (Rehwinkel et al., 2005) The orientation of the Dcr-2/r2d2 protein heterodimer on the siRNA duplex determines which siRNA strand associates with the core RISC (RNA-induced silencing complex) protein AGO2. r2d2 protein binds the siRNA end with the greatest double-stranded character, thereby orienting the Dcr-2/r2d2 heterodimer on the siRNA duplex. Strong binding by the r2d2 protein requires a 5'-phosphate on the siRNA strand that is excluded from the RISC. (Tomari et al., 2004)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_140518 NM_168626 RefSeq (Proteins) • NP_648775 NP_730054 DDBJ / EMBL / GenBank • AA803111 AAF49619 AAF49620 AAM11104 AAO39550 ABB54719 ABB54720 ABB54721 ABB54722 ABB54723 ABB54724 ABB54725 ABB54726 AI945716 AW941283 AY094751 BI164635 BT003546 CL527754 DQ228766 DQ228767 DQ228768 DQ228769 DQ228770 DQ228771 DQ228772 DQ228773 Entrez Gene - A searchable database of RefSeq genes. • 39683 UniProtKB/Swiss-Prot • Q9VUQ5
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Argonaute/Dicer protein, PAZ (IPR003100) Stem cell self-renewal protein Piwi (IPR003165) Ribonuclease H-like (IPR012337) Domain of unknown function DUF1785 (IPR014811)
Linkouts
	BioGRID - A database of protein and genetic interactions • 65002 DroID - A comprehensive database of gene and protein interactions. • FBgn0087035 DRSC - Results from RNAi screens. • FBgn0087035 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0087035 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0087035 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 39683 45982 InParanoid A subset of ortholog calls from InParanoid. • AGAP011537-PA Anopheles gambiae (mosquito) XP_395048.3 Apis mellifera (honey bee) XP_001944852.1 Acyrthosiphon pisum (pea aphid) BGIBMGA010406-PA Bombyx mori (silk worm) CPIJ009898 Culex pipens (mosquito) XP_001607164.1 Nasonia vitripennis (wasp) PHUM002582-PA Pediculus humanus (human body louse) NP_001107828.1 Tribolium castaneum (red flour beetle) 20630 Thalassiosira pseudonana (marine diatom) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /sturtevant/argonaut2-1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 39683 modMine - Data generated by the modENCODE project. • FBgn0087035 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB16541 NV12825 NV12824 LH23016 FBgn0085112 FBgn0212615 FBgn0195702 FBgn0084451 FBgn0180394 FBgn0245029 FBgn0162465 FBgn0135876 FBgn0122217 FBgn0108142 FBgn0087097 CPIJ014791 CPIJ009898 BGIBMGA010406 ACYPI008293 GB15464 AGAP011537 AAEL017251 PHUM004130 GLEAN_11525 GLEAN_13762
Synonyms & Secondary IDs ( 23 )
Reported As
Symbol Synonym	AG02 (Foley and O'Farrell, 2004) ago (Muller, 2010.6.29) Ago2 (Zamore, 2007, Meyer et al., 2006, Filipowicz, 2005, Li and Ding, 2005, Hammond, 2005, Zamore and Haley, 2005, Tomari et al., 2004, Mason et al., 2004, Skipper, 2004, Pham et al., 2004, Du et al., 2006, Pham and Sontheimer, 2005, Obbard et al., 2006, Sommer, 2006, Zaratiegui, 2007, Preall and Sontheimer, 2005, Matranga et al., 2005, Rand et al., 2005, Vagin et al., 2006, Valencia-Sanchez et al., 2006, Lingel et al., 2003, Okamura et al., 2007, Tomari et al., 2007, Jacquier et al., 2008, González et al., 2008, Peng and Karpen, 2007, Borah et al., 2009, Saleh et al., 2009, Zhou et al., 2008, Berry et al., 2009, Sabin et al., 2009, Iwasaki et al., 2009, Li et al., 2009, Kim et al., 2007, Easow et al., 2007, Obbard et al., 2009, Pepper et al., 2009, Nayak et al., 2010, Glaser and Meola, 2010, Ghildiyal et al., 2010, González et al., 2010, Shah and Förstemann, 2008, Ameres et al., 2011, Iwasaki et al., 2010, Lingel et al., 2004, Miyoshi et al., 2010) AGO2 (Grewal and Elgin, 2007, Dorner et al., 2006, Rehwinkel et al., 2005, Ulvila et al., 2006, Gunawardane et al., 2007, Czech et al., 2009, Robine et al., 2009, Grieder et al., 2007, Jin et al., 2004, Brower-Toland et al., 2007, Rehwinkel et al., 2006, Czech et al., 2008, Okamura et al., 2008, Kawamura et al., 2008, Berdnik et al., 2008, Haley et al., 2008, Reich et al., 2009, Eulalio et al., 2007, Deddouche et al., 2008, Hurt et al., 2009, Taft et al., 2009, Habayeb et al., 2009, Teleman et al., 2008, Siomi et al., 2005, Nishida et al., 2007, Behm-Ansmant et al., 2006, Graze et al., 2009, Okamura et al., 2009, Curtis et al., 2007, Moshkovich and Lei, 2010, Obbard et al., 2011, Mueller et al., 2010, Okamura et al., 2011, Ni et al., 2011, Khong and Jan, 2011, Jovel and Schneemann, 2011) ago2 (Pek and Kai, 2011, Hartig et al., 2009, Meyer et al., 2005, Xu et al., 2004, Banerjee and Slack, 2002, Kavi and Birchler, 2007, Vagin et al., 2006, Förstemann et al., 2007, Kavi and Birchler, 2008, Horwich et al., 2007, Ghildiyal et al., 2008, Tadros et al., 2007, Chung et al., 2008, Blumenstiel et al., 2008, Martin et al., 2009, Flynt et al., 2009, Hain et al., 2010, Kadener et al., 2009, Chambeyron et al., 2008, Rehwinkel et al., 2005, Fagegaltier et al., 2009, Lucchetta et al., 2009, Liu et al., 2009, Czech et al., 2009, Liu and Gall, 2007, Ameres et al., 2010, Pek and Kai, 2011, Gehrke et al., 2010, Marques et al., 2010, Singh et al., 2011, Seitz et al., 2011, Khurana et al., 2010) Ago-2 (Waterhouse et al., 2007, Hutvagner and Zamore, 2002, Caudy et al., 2002, Hammond et al., 2001, Budde, 2006, Budde, 2006, Sanders and Smith, 2011, van Rij et al., 2006, Saleh et al., 2006, Lipardi and Paterson, 2009, Mukherjee and Hanley, 2010) ago-2 (Deshpande et al., 2005, Wang et al., 2006, Wang et al., 2006, Jepson and Reenan, 2009) AGO 2 (Ulvila et al., 2005) CG7439 (Gorski et al., 2003, Ramet et al., 2002, Labourier et al., 2002, Harris and Macdonald, 2001, Kataoka et al., 2001, Dorner et al., 2006, Rehwinkel et al., 2005, Grieder et al., 2007, González et al., 2008, Saleh et al., 2006, Keleman et al., 2009.8.5, Ni et al., 2011.7.19) CG13452   dAgo2 (Jing et al., 2005, Denli and Hannon, 2003, Carmell et al., 2002, Gehrke et al., 2010) dAGO2 (Monzo et al., 2006) Dm Ago2 (Schwarz and Zamore, 2002) eIF2C2 (Wienholds, 2005)
Name Synonym	Argonaute (Jacquier et al., 2008, Easow et al., 2007) argonaute (Barbee et al., 2006) argonaute 2 (Muller, 2010.6.29) Argonaute 2 (Hammond et al., 2001, Ulvila et al., 2006, Lingel et al., 2003, Schneider et al., 2006, van Rij et al., 2006, Jin et al., 2004, Okamura et al., 2008, Kawamura et al., 2008, Saleh et al., 2006, Saleh et al., 2009, Worringer et al., 2009, Graze et al., 2009, Harbison et al., 2005, Nayak et al., 2010, Mukherjee and Hanley, 2010, Miyoshi et al., 2010, Maiti et al., 2011) Argonaute2 (Vagin et al., 2006, Simmonds et al., 2006, Du et al., 2006, Lei, 2007, Carmi, 2006, Dorner et al., 2006, Lei et al., 2008, Iwasaki et al., 2009, Kim et al., 2007, Pepper et al., 2009, Liu and Gall, 2007, Ameres et al., 2010, Ghildiyal et al., 2010, Lingel et al., 2004) Argonaute-2 (Wang et al., 2006, Czech et al., 2008, Chung et al., 2008, Berdnik et al., 2008, Sofola et al., 2007, Gerbasi et al., 2010, Obbard et al., 2011) argonaute2 (Hain et al., 2010) ARGONAUTE2 (Lucchetta et al., 2009) argonaute-2 (Jepson and Reenan, 2009)
Secondary FlyBase IDs
	FBgn0004891 FBgn0036508 FBgn0040803 FBgn0046812
References ( 217 )
Generate a list of	All references relating to this gene ( 217 )
List References by type	Research paper  ( 133 ) Supplementary material  ( 8 ) Review  ( 34 ) Personal communication to FlyBase  ( 7 ) Abstract  ( 10 ) FlyBase analysis  ( 4 ) Meeting report  ( 1 ) Computer file  ( 2 ) DNA/RNA sequence record  ( 18 )
Recent research papers ( 28 )
	Ameres et al., 2011, RNA 17(1): 54--63 Target RNA-directed tailing and trimming purifies the sorting of endo-siRNAs between the two Drosophila Argonaute proteins. [FBrf0212657] Jovel and Schneemann, 2011, Virology 419(1): 43--53 Molecular characterization of Drosophila cells persistently infected with Flock House virus. [FBrf0215224] Khong and Jan, 2011, J. Virol. 85(4): 1439--1451 Modulation of Stress Granules and P Bodies during Dicistrovirus Infection. [FBrf0212847] Maiti et al., 2011, Chemistry 17(5): 1519--1528 Structural and Binding Study of Modified siRNAs with the Argonaute 2 PAZ Domain by NMR Spectroscopy. [FBrf0212887] Moshkovich et al., 2011, Genes Dev. 25(16): 1686--1701 RNAi-independent role for Argonaute2 in CTCF/CP190 chromatin insulator function. [FBrf0214686] Ni et al., 2011, Nat. Methods 8(5): 405--407 A genome-scale shRNA resource for transgenic RNAi in Drosophila. [FBrf0213581] Obbard et al., 2011, Mol. Biol. Evol. 28(2): 1043--1056 Recent and Recurrent Selective Sweeps of the Antiviral RNAi Gene Argonaute-2 in Three Species of Drosophila. [FBrf0212800] Okamura et al., 2011, Mol. Cell. Biol. 31(4): 884--896 R2D2 Organizes Small Regulatory RNA Pathways in Drosophila. [FBrf0212879] Pek and Kai, 2011, Proc. Natl. Acad. Sci. U.S.A. 108(29): 12007--12012 DEAD-box RNA helicase Belle/DDX3 and the RNA interference pathway promote mitotic chromosome segregation. [FBrf0214405] Pek and Kai, 2011, Curr. Biol. 21(1): 39--44 A role for vasa in regulating mitotic chromosome condensation in Drosophila. [FBrf0212718] Sanders and Smith, 2011, PLoS ONE 6(8): e24151 LUMP Is a Putative Double-Stranded RNA Binding Protein Required for Male Fertility in Drosophila melanogaster. [FBrf0215206] Seitz et al., 2011, Silence 2: 4 A 5'-uridine amplifies miRNA/miRNA* asymmetry in Drosophila by promoting RNA-induced silencing complex formation. [FBrf0214042] Singh et al., 2011, Dev. Biol. 352(1): 104--115 The Bin3 RNA methyltransferase is required for repression of caudal translation in the Drosophila embryo. [FBrf0213172] Ameres et al., 2010, Science 328(5985): 1534--1539 Target RNA-directed trimming and tailing of small silencing RNAs. [FBrf0211070] Gehrke et al., 2010, Nature 466(7306): 637--641 Pathogenic LRRK2 negatively regulates microRNA-mediated translational repression. [FBrf0211387] Gerbasi et al., 2010, Mol. Cell. Proteomics 9(9): 1866--1872 Proteomics Identification of Drosophila Small Interfering RNA-associated Factors. [FBrf0211707] Ghildiyal et al., 2010, RNA 16(1): 43--56 Sorting of Drosophila small silencing RNAs partitions microRNA* strands into the RNA interference pathway. [FBrf0209572] Glaser and Meola, 2010, PLoS ONE 5(8): e11977 The Native Wolbachia Endosymbionts of Drosophila melanogaster and Culex quinquefasciatus Increase Host Resistance to West Nile Virus Infection. [FBrf0211492] González et al., 2010, PLoS Genet. 6(4): e1000905 Genome-wide patterns of adaptation to temperate environments associated with transposable elements in Drosophila. [FBrf0210506] Hain et al., 2010, PLoS ONE 5(12): e15264 Natural variation of the amino-terminal glutamine-rich domain in Drosophila argonaute2 is not associated with developmental defects. [FBrf0212816] Iwasaki et al., 2010, Mol. Cell 39(2): 292--299 Hsc70/Hsp90 chaperone machinery mediates ATP-dependent RISC loading of small RNA duplexes. [FBrf0214214] Khurana et al., 2010, PLoS Genet. 6(12): e1001246 Distinct Functions for the Drosophila piRNA Pathway in Genome Maintenance and Telomere Protection. [FBrf0212661] Marques et al., 2010, Nat. Struct. Mol. Biol. 17(1): 24--30 Loqs and R2D2 act sequentially in the siRNA pathway in Drosophila. [FBrf0209625] Miyoshi et al., 2010, Nat. Struct. Mol. Biol. 17(8): 1024--1026 A direct role for Hsp90 in pre-RISC formation in Drosophila. [FBrf0211382] Moshkovich and Lei, 2010, PLoS Genet. 6(3): e1000880 HP1 Recruitment in the Absence of Argonaute Proteins in Drosophila. [FBrf0210291] Mueller et al., 2010, Proc. Natl. Acad. Sci. U.S.A. 107(45): 19390--19395 RNAi-mediated immunity provides strong protection against the negative-strand RNA vesicular stomatitis virus in Drosophila. [FBrf0212288] Mukherjee and Hanley, 2010, BMC Microbiol. 10: 127 RNA interference modulates replication of dengue virus in Drosophila melanogaster cells. [FBrf0210847] Nayak et al., 2010, Nat. Struct. Mol. Biol. 17(5): 547--554 Cricket paralysis virus antagonizes Argonaute 2 to modulate antiviral defense in Drosophila. [FBrf0210742] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...