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Gene Dmel\puc

General Information
Symbol	Dmel\puc	Species	D. melanogaster
Name	puckered	Annotation symbol	CG7850
Feature type	protein_coding_gene	FlyBase ID	FBgn0243512
Gene Model Status	Current	Stock availability	27 publicly available
Also Known As	hrt, l(3)84Eh
Genomic Location
Chromosome (arm)	3R	Recombination map	3-47.6
Cytogenetic map	84E12-84E11	Sequence location	3R:3,931,054..3,948,016 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene puckered is referred to in FlyBase by the symbol Dmel\puc (CG7850, FBgn0243512). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: JUN kinase phosphatase activity; phosphoprotein phosphatase activity. There is experimental evidence for 24 unique biological process terms, many of which group under: single-organism developmental process; biological regulation; cellular component organization or biogenesis; localization; post-embryonic organ morphogenesis; response to stress; ovarian follicle cell development; system development; digestive tract development; actin filament-based process. 59 alleles are reported. The phenotypes of these alleles are annotated with: organ system; organ system subdivision; acellular anatomical structure; multicellular structure; external compound sense organ; adult segment; non-connected developing system; portion of tissue; imaginal precursor; peripheral nervous system. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Dual specificity phosphatase; Dual specificity phosphatase, catalytic domain; Dual specificity phosphatase, subgroup, catalytic domain; Protein-tyrosine phosphatase, active site; Protein-tyrosine/Dual specificity phosphatase. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 12-24 hour embryonic stages, during late larval stages, during early pupal stages. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: adult midgut, adult Malpighian tubules, adult salivary gland. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval midgut, larval/adult hindgut, larval Malpighian tubules, adult heart, adult fat body, larval salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1631765_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of puc. Gene sequence location is 3R:3931054..3948016.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02	Controlled Vocabulary Terms inactivation of MAPK activity
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 84E12-84E11   Limits computationally determined from genome sequence between P{EP}EP3060EP3060 and P{PZ}grn05930; Left limit from in situ hybridisation (FBrf0067338) Right limit from in situ hybridisation (FBrf0067338)
Experimentally Determined Cytological Location
Cytogenetic map Notes References 84E-84E     (Martin-Blanco, 1998.1.12) 84E10-84E11   (determined by in situ hybridisation)   (Spradling et al., 1999) 84F-84F   (determined by in situ hybridisation)   (Szeged Stock Center, 1998-) 84E-84E   (determined by in situ hybridisation)   (Martin-Blanco et al., 1998) 84F-84F   (determined by in situ hybridisation)   (Salzberg et al., 1997) 84F-84F   (determined by in situ hybridisation)   (Deak et al., 1997) 84F1-84F2   (determined by in situ hybridisation)   (Salzberg et al., 1994) 84E-84E   (determined by in situ hybridisation)   (Ring and Martinez Arias, 1993) 84E10-84E11   (determined by in situ hybridisation)   (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location	3-47.6 (Salzberg et al., 1994)
Left of (cM)	cu (Kirchner et al., 2007)
Right of (cM)	st (Kirchner et al., 2007)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\puc for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.49
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) puc-RA FBtr0081792  NM_079549   2919   476 puc-RB FBtr0339722     2407   369
Additional Transcript Data & Comments
Reported size (kB)	2.9 (northern blot) (Martin-Blanco et al., 1998)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein puc-PA   FBpp0081288   51.3   476   6.02     NP_524273   AAF54191 puc-PB   FBpp0308778   39.5   369   5.41
Additional Polypeptide Data & Comments
Reported size (kDa)	476 (aa); 58 (kD predicted) (Martin-Blanco et al., 1998)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Protein-tyrosine/Dual specificity phosphatase (IPR000387) Dual specificity phosphatase, catalytic domain (IPR000340) Protein-tyrosine phosphatase, active site (IPR016130) Dual specificity phosphatase, subgroup, catalytic domain (IPR020422) Dual specificity phosphatase (IPR024950)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014297 AAF54191   AF174657   AI542084   AJ223360 CAA11282   AQ025202   AQ026320   AW944549   AY061616 AAL29164   BG640704   BH615123   BI164896   BI213153   BI363232   BI578977   BI633662   BI637842   BP545915   BP546795   BP548981   CL610255   CO178872   CO181025   CO185431   CO187497   CO187923   CO278148   CO292849   CO292878   CO312520   CO316811   CO340013   CO340082   CZ468616   CZ469169   CZ469798   CZ470972   CZ476486   CZ477654   CZ477799   CZ479140   CZ485418   CZ487585   CZ488867   EC051594   EC052078   EC053107   EC053592   EC055128   EC055771   EC056603   EC057594   EC057595   EC076450   EC076873   EC086612   EC086617   EC089474   EC206311   EC209964   EC218646   EC228871   EC246663   EC256502   EV595288   GH749983   GH753012   GH754999   GH756416   GH771570   GH772903   GH775179   GH783344   GH784926   GH795656   GH797090   GH801996   GH802813   GH809841   GH819566   GH820966   GH822676   GH824100   GH825067   GH828412   GH831526   GH834305   GH835885   GH837714   GH842791   GH843604   GH845963   GH847253   GH859002   GH862058   GH869692   GH877146   GH878149   GH887330   GH895574   GH896130   GH908925   GH909414   GH914732   GH921047   GH925846
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VHV8 O46122
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 9 -- 12   epidermis | dorsal   (Martin-Blanco et al., 1998) Comment:reference states 4-8 hr AEL northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage     (Martin-Blanco et al., 1998)
Additional Descriptive Data
	puc transcripts are detected throughout embryogenesis by northern blot. By in situ hybridization, puc transcripts are detected weakly in the dorsal-most cells at the leading edge of the epidermis. After stage 11, expression decays. (Martin-Blanco et al., 1998)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
endoplasmic reticulum inferred from direct assay (Bard et al., 2006) Golgi apparatus inferred from direct assay (Bard et al., 2006)
Expression Deduced from Reporters
Reporter: P{A92}pucE69 Stage Tissue/Position (including subcellular localization) Reference embryonic stage   leading edge mixer cell | subset   (Gettings et al., 2010) embryonic stage 11 -- 15   amnioserosa | ventral to   (Ring and Martinez Arias, 1993) embryonic stage 11 -- 17   epidermis | dorsal   (Ring and Martinez Arias, 1993) embryonic stage 13 -- 16   embryonic leading edge cell   (Stronach and Perrimon, 2001) embryonic stage 14   embryonic leading edge cell   (Tateno et al., 2000) embryonic stage 14 -- 15   embryonic leading edge cell   (Magie et al., 1999) embryonic stage 15   embryonic leading edge cell   (Goberdhan and Wilson, 1998) third instar larval stage   wing disc | restricted   (del Alamo Rodriguez et al., 2004) Reporter: P{lArB}pucA251.1F3 Stage Tissue/Position (including subcellular localization) Reference embryonic stage   epidermis | dorsal   (Bellen, 1998.8.10) amnioserosa | vicinity of   (Bellen, 1998.8.10) larval stage   ventral thoracic disc | distal   (Azpiazu and Morata, 2002) adult stage & oogenesis stage S10   follicle cell   (Bellen, 1998.8.10)
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: adult midgut, adult Malpighian tubules, adult salivary gland. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval midgut, larval/adult hindgut, larval Malpighian tubules, adult heart, adult fat body, larval salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    260.1 Larval Midgut    462.9 Larval Hindgut    353.1 Larval Malpighian Tubules    226 Larval Fat Body    99.5 Larval Salivary Gland    321.5 Larval Trachea    267.6 Larval Carcass    362.65 Adult Head    235.4 Adult Eye    345.325 Adult Brain    183.6 Adult Thoracic-Abdominal Ganglion    183.4 Adult Crop    472.8 Adult Midgut    562 Adult Hindgut    328.8 Adult Malpighian Tubules    552.8 Adult Fat Body    192.7 Adult Salivary Gland    798.9 Adult Heart    183.525 Adult VirginFemale Spermatheca    414.2 Adult InseminatedFemale Spermatheca    419.9 Adult Ovary    227.5 Adult Testis    21.9 Adult Male Accessory Gland    106.5 Adult Carcass    163.4 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    260.1 Larval Midgut    462.9 Larval Hindgut    353.1 Larval Malpighian Tubules    226 Larval Fat Body    99.5 Larval Salivary Gland    321.5 Larval Trachea    267.6 Larval Carcass    362.65 Adult Head    235.4 Adult Eye    345.325 Adult Brain    183.6 Adult Thoracic-Abdominal Ganglion    183.4 Adult Crop    472.8 Adult Midgut  (562) Adult Hindgut    328.8 Adult Malpighian Tubules  (552.8) Adult Fat Body    192.7 Adult Salivary Gland  (798.9) Adult Heart    183.525 Adult VirginFemale Spermatheca    414.2 Adult InseminatedFemale Spermatheca    419.9 Adult Ovary    227.5 Adult Testis    21.9 Adult Male Accessory Gland    106.5 Adult Carcass    163.4 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    260.1 Larval Midgut    462.9 Larval Hindgut    353.1 Larval Malpighian Tubules    226 Larval Fat Body    99.5 Larval Salivary Gland    321.5 Larval Trachea    267.6 Larval Carcass    362.65 Adult Head    235.4 Adult Eye    345.325 Adult Brain    183.6 Adult Thoracic-Abdominal Ganglion    183.4 Adult Crop    472.8 Adult Midgut    562 Adult Hindgut    328.8 Adult Malpighian Tubules    552.8 Adult Fat Body    192.7 Adult Salivary Gland    798.9 Adult Heart    183.525 Adult VirginFemale Spermatheca    414.2 Adult InseminatedFemale Spermatheca    419.9 Adult Ovary    227.5 Adult Testis    21.9 Adult Male Accessory Gland    106.5 Adult Carcass    163.4 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    260.1 Larval Midgut    462.9 Larval Hindgut    353.1 Larval Malpighian Tubules    226 Larval Fat Body    99.5 Larval Salivary Gland    321.5 Larval Trachea    267.6 Larval Carcass    362.65 Adult Head    235.4 Adult Eye    345.325 Adult Brain    183.6 Adult Thoracic-Abdominal Ganglion    183.4 Adult Crop    472.8 Adult Midgut    562 Adult Hindgut    328.8 Adult Malpighian Tubules    552.8 Adult Fat Body    192.7 Adult Salivary Gland    798.9 Adult Heart    183.525 Adult VirginFemale Spermatheca    414.2 Adult InseminatedFemale Spermatheca    419.9 Adult Ovary    227.5 Adult Testis    21.9 Adult Male Accessory Gland    106.5 Adult Carcass    163.4 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    260.1 Larval Midgut    462.9 Larval Hindgut    353.1 Larval Malpighian Tubules    226 Larval Fat Body    99.5 Larval Salivary Gland    321.5 Larval Trachea    267.6 Larval Carcass    362.65 Adult Head    235.4 Adult Eye    345.325 Adult Brain    183.6 Adult Thoracic-Abdominal Ganglion    183.4 Adult Crop    472.8 Adult Midgut    562 Adult Hindgut    328.8 Adult Malpighian Tubules    552.8 Adult Fat Body    192.7 Adult Salivary Gland    798.9 Adult Heart    183.525 Adult VirginFemale Spermatheca    414.2 Adult InseminatedFemale Spermatheca    419.9 Adult Ovary    227.5 Adult Testis    21.9 Adult Male Accessory Gland    106.5 Adult Carcass    163.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    260.1 Larval Midgut    462.9 Larval Hindgut    353.1 Larval Malpighian Tubules    226 Larval Fat Body    99.5 Larval Salivary Gland    321.5 Larval Trachea    267.6 Larval Carcass    362.65 Adult Head    235.4 Adult Eye    345.325 Adult Brain    183.6 Adult Thoracic-Abdominal Ganglion    183.4 Adult Crop    472.8 Adult Midgut  562 Adult Hindgut    328.8 Adult Malpighian Tubules  552.8 Adult Fat Body    192.7 Adult Salivary Gland  798.9 Adult Heart    183.525 Adult VirginFemale Spermatheca    414.2 Adult InseminatedFemale Spermatheca    419.9 Adult Ovary    227.5 Adult Testis    21.9 Adult Male Accessory Gland    106.5 Adult Carcass    163.4 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    260.1 Larval Midgut    462.9 Larval Hindgut    353.1 Larval Malpighian Tubules    226 Larval Fat Body    99.5 Larval Salivary Gland    321.5 Larval Trachea    267.6 Larval Carcass    362.65 Adult Head    235.4 Adult Eye    345.325 Adult Brain    183.6 Adult Thoracic-Abdominal Ganglion    183.4 Adult Crop    472.8 Adult Midgut    562 Adult Hindgut    328.8 Adult Malpighian Tubules    552.8 Adult Fat Body    192.7 Adult Salivary Gland    798.9 Adult Heart    183.525 Adult VirginFemale Spermatheca    414.2 Adult InseminatedFemale Spermatheca    419.9 Adult Ovary    227.5 Adult Testis    21.9 Adult Male Accessory Gland    106.5 Adult Carcass    163.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    260.1 Larval Midgut    462.9 Larval Hindgut    353.1 Larval Malpighian Tubules    226 Larval Fat Body    99.5 Larval Salivary Gland    321.5 Larval Trachea    267.6 Larval Carcass    362.65 Adult Head    235.4 Adult Eye    345.325 Adult Brain    183.6 Adult Thoracic-Abdominal Ganglion    183.4 Adult Crop    472.8 Adult Midgut    562 Adult Hindgut    328.8 Adult Malpighian Tubules    552.8 Adult Fat Body    192.7 Adult Salivary Gland    798.9 Adult Heart    183.525 Adult VirginFemale Spermatheca    414.2 Adult InseminatedFemale Spermatheca    419.9 Adult Ovary    227.5 Adult Testis    21.9 Adult Male Accessory Gland    106.5 Adult Carcass    163.4 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    235.4   NA  Eye    345.325   NA  Brain    183.6   260.1  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    183.4   NA  Crop    472.8   462.9  Midgut    562   353.1  Hindgut    328.8   226  Malpighian Tubules    552.8   99.5  Fat Body    192.7   321.5  Salivary Gland    798.9   NA  Heart    183.525   267.6  Trachea    NA   NA  VirginFemale Spermatheca    414.2   NA  InseminatedFemale Spermatheca    419.9   NA  Ovary    227.5   NA  Testis    21.9   NA  Male Accessory Gland    106.5   362.65  Carcass    163.4 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    24 central nervous system, larvae L3    33 central nervous system, pupae P8    22 head, virgin 1-day female    25 head, virgin 4-day female    37 head, virgin 20-day female    24 head, mated 1-day female    36 head, mated 4-day female    38 head, mated 20-day female    26 head, mated 1-day male    40 head, mated 4-day male    63 head, mated 20-day male    51 salivary gland, larvae L3 wandering    9 salivary gland, white prepupae    52 digestive system, larvae L3 wandering    45 digestive system, 1-day adult    45 digestive system, 4-day adult    66 digestive system, 20-day adult    66 fat body, larvae L3 wandering    15 fat body, white prepupae    14 fat body, pupae P8    28 carcass, larvae L3 wandering    33 carcass, 1-day adult    29 carcass, 4-day adult    31 carcass, 20-day adult    30 ovary, virgin 4-day female    26 ovary, mated 4-day female    29 testis, mated 4-day male    5 accessory gland, mated 4-day male    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    24 central nervous system, larvae L3  (33) central nervous system, pupae P8    22 head, virgin 1-day female    25 head, virgin 4-day female  (37) head, virgin 20-day female    24 head, mated 1-day female  (36) head, mated 4-day female  (38) head, mated 20-day female  26 head, mated 1-day male  (40) head, mated 4-day male  (63) head, mated 20-day male  (51) salivary gland, larvae L3 wandering    9 salivary gland, white prepupae  (52) digestive system, larvae L3 wandering  (45) digestive system, 1-day adult  (45) digestive system, 4-day adult  (66) digestive system, 20-day adult  (66) fat body, larvae L3 wandering    15 fat body, white prepupae    14 fat body, pupae P8  (28) carcass, larvae L3 wandering  (33) carcass, 1-day adult  (29) carcass, 4-day adult  (31) carcass, 20-day adult  (30) ovary, virgin 4-day female  26 ovary, mated 4-day female  (29) testis, mated 4-day male    5 accessory gland, mated 4-day male    14 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    24 central nervous system, larvae L3    33 central nervous system, pupae P8    22 head, virgin 1-day female    25 head, virgin 4-day female    37 head, virgin 20-day female    24 head, mated 1-day female    36 head, mated 4-day female    38 head, mated 20-day female    26 head, mated 1-day male    40 head, mated 4-day male    63 head, mated 20-day male    51 salivary gland, larvae L3 wandering    9 salivary gland, white prepupae    52 digestive system, larvae L3 wandering    45 digestive system, 1-day adult    45 digestive system, 4-day adult    66 digestive system, 20-day adult    66 fat body, larvae L3 wandering    15 fat body, white prepupae    14 fat body, pupae P8    28 carcass, larvae L3 wandering    33 carcass, 1-day adult    29 carcass, 4-day adult    31 carcass, 20-day adult    30 ovary, virgin 4-day female    26 ovary, mated 4-day female    29 testis, mated 4-day male    5 accessory gland, mated 4-day male    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    24 central nervous system, larvae L3    33 central nervous system, pupae P8    22 head, virgin 1-day female    25 head, virgin 4-day female    37 head, virgin 20-day female    24 head, mated 1-day female    36 head, mated 4-day female    38 head, mated 20-day female    26 head, mated 1-day male    40 head, mated 4-day male    63 head, mated 20-day male    51 salivary gland, larvae L3 wandering    9 salivary gland, white prepupae    52 digestive system, larvae L3 wandering    45 digestive system, 1-day adult    45 digestive system, 4-day adult    66 digestive system, 20-day adult    66 fat body, larvae L3 wandering    15 fat body, white prepupae    14 fat body, pupae P8    28 carcass, larvae L3 wandering    33 carcass, 1-day adult    29 carcass, 4-day adult    31 carcass, 20-day adult    30 ovary, virgin 4-day female    26 ovary, mated 4-day female    29 testis, mated 4-day male    5 accessory gland, mated 4-day male    14 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    24 central nervous system, larvae L3    33 central nervous system, pupae P8    22 head, virgin 1-day female    25 head, virgin 4-day female    37 head, virgin 20-day female    24 head, mated 1-day female    36 head, mated 4-day female    38 head, mated 20-day female    26 head, mated 1-day male    40 head, mated 4-day male    63 head, mated 20-day male    51 salivary gland, larvae L3 wandering    9 salivary gland, white prepupae    52 digestive system, larvae L3 wandering    45 digestive system, 1-day adult    45 digestive system, 4-day adult    66 digestive system, 20-day adult    66 fat body, larvae L3 wandering    15 fat body, white prepupae    14 fat body, pupae P8    28 carcass, larvae L3 wandering    33 carcass, 1-day adult    29 carcass, 4-day adult    31 carcass, 20-day adult    30 ovary, virgin 4-day female    26 ovary, mated 4-day female    29 testis, mated 4-day male    5 accessory gland, mated 4-day male    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    24 central nervous system, larvae L3  (33) central nervous system, pupae P8    22 head, virgin 1-day female    25 head, virgin 4-day female  (37) head, virgin 20-day female    24 head, mated 1-day female  (36) head, mated 4-day female  (38) head, mated 20-day female  26 head, mated 1-day male  (40) head, mated 4-day male  (63) head, mated 20-day male  (51) salivary gland, larvae L3 wandering    9 salivary gland, white prepupae  (52) digestive system, larvae L3 wandering  (45) digestive system, 1-day adult  (45) digestive system, 4-day adult  (66) digestive system, 20-day adult  (66) fat body, larvae L3 wandering    15 fat body, white prepupae    14 fat body, pupae P8  28 carcass, larvae L3 wandering  (33) carcass, 1-day adult  29 carcass, 4-day adult  31 carcass, 20-day adult  30 ovary, virgin 4-day female  26 ovary, mated 4-day female  29 testis, mated 4-day male    5 accessory gland, mated 4-day male    14 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    24 central nervous system, larvae L3    33 central nervous system, pupae P8    22 head, virgin 1-day female    25 head, virgin 4-day female    37 head, virgin 20-day female    24 head, mated 1-day female    36 head, mated 4-day female    38 head, mated 20-day female    26 head, mated 1-day male    40 head, mated 4-day male    63 head, mated 20-day male    51 salivary gland, larvae L3 wandering    9 salivary gland, white prepupae    52 digestive system, larvae L3 wandering    45 digestive system, 1-day adult    45 digestive system, 4-day adult    66 digestive system, 20-day adult    66 fat body, larvae L3 wandering    15 fat body, white prepupae    14 fat body, pupae P8    28 carcass, larvae L3 wandering    33 carcass, 1-day adult    29 carcass, 4-day adult    31 carcass, 20-day adult    30 ovary, virgin 4-day female    26 ovary, mated 4-day female    29 testis, mated 4-day male    5 accessory gland, mated 4-day male    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    24 central nervous system, larvae L3    33 central nervous system, pupae P8    22 head, virgin 1-day female    25 head, virgin 4-day female    37 head, virgin 20-day female    24 head, mated 1-day female    36 head, mated 4-day female    38 head, mated 20-day female    26 head, mated 1-day male    40 head, mated 4-day male    63 head, mated 20-day male    51 salivary gland, larvae L3 wandering    9 salivary gland, white prepupae    52 digestive system, larvae L3 wandering    45 digestive system, 1-day adult    45 digestive system, 4-day adult    66 digestive system, 20-day adult    66 fat body, larvae L3 wandering    15 fat body, white prepupae    14 fat body, pupae P8    28 carcass, larvae L3 wandering    33 carcass, 1-day adult    29 carcass, 4-day adult    31 carcass, 20-day adult    30 ovary, virgin 4-day female    26 ovary, mated 4-day female    29 testis, mated 4-day male    5 accessory gland, mated 4-day male    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 12-24 hour embryonic stages, during late larval stages, during early pupal stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    16 embryo 02-04hr    9 embryo 04-06hr    9 embryo 06-08hr    11 embryo 08-10hr    11 embryo 10-12hr    14 embryo 12-14hr    19 embryo 14-16hr    22 embryo 16-18hr    28 embryo 18-20hr    36 embryo 20-22hr    31 embryo 22-24hr    32 larva L1    13 larva L2    10 larva L3 12hr old    7 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    21 white prepupae new    25 white prepupae 12hr    26 white prepupae 24hr    13 pupae 2d postWPP    13 pupae 3d postWPP    7 pupae 4d postWPP    11 adult male 01day    10 adult male 05day    12 adult male 30day    12 adult female 01day    11 adult female 05day    13 adult female 30day    13 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    16 embryo 02-04hr    9 embryo 04-06hr    9 embryo 06-08hr    11 embryo 08-10hr    11 embryo 10-12hr    14 embryo 12-14hr    19 embryo 14-16hr    22 embryo 16-18hr  (28) embryo 18-20hr  (36) embryo 20-22hr  (31) embryo 22-24hr  (32) larva L1    13 larva L2    10 larva L3 12hr old    7 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    21 white prepupae new    25 white prepupae 12hr  26 white prepupae 24hr    13 pupae 2d postWPP    13 pupae 3d postWPP    7 pupae 4d postWPP    11 adult male 01day    10 adult male 05day    12 adult male 30day    12 adult female 01day    11 adult female 05day    13 adult female 30day    13 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    16 embryo 02-04hr    9 embryo 04-06hr    9 embryo 06-08hr    11 embryo 08-10hr    11 embryo 10-12hr    14 embryo 12-14hr    19 embryo 14-16hr    22 embryo 16-18hr    28 embryo 18-20hr    36 embryo 20-22hr    31 embryo 22-24hr    32 larva L1    13 larva L2    10 larva L3 12hr old    7 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    21 white prepupae new    25 white prepupae 12hr    26 white prepupae 24hr    13 pupae 2d postWPP    13 pupae 3d postWPP    7 pupae 4d postWPP    11 adult male 01day    10 adult male 05day    12 adult male 30day    12 adult female 01day    11 adult female 05day    13 adult female 30day    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    16 embryo 02-04hr    9 embryo 04-06hr    9 embryo 06-08hr    11 embryo 08-10hr    11 embryo 10-12hr    14 embryo 12-14hr    19 embryo 14-16hr    22 embryo 16-18hr    28 embryo 18-20hr    36 embryo 20-22hr    31 embryo 22-24hr    32 larva L1    13 larva L2    10 larva L3 12hr old    7 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    21 white prepupae new    25 white prepupae 12hr    26 white prepupae 24hr    13 pupae 2d postWPP    13 pupae 3d postWPP    7 pupae 4d postWPP    11 adult male 01day    10 adult male 05day    12 adult male 30day    12 adult female 01day    11 adult female 05day    13 adult female 30day    13 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    16 embryo 02-04hr    9 embryo 04-06hr    9 embryo 06-08hr    11 embryo 08-10hr    11 embryo 10-12hr    14 embryo 12-14hr    19 embryo 14-16hr    22 embryo 16-18hr    28 embryo 18-20hr    36 embryo 20-22hr    31 embryo 22-24hr    32 larva L1    13 larva L2    10 larva L3 12hr old    7 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    21 white prepupae new    25 white prepupae 12hr    26 white prepupae 24hr    13 pupae 2d postWPP    13 pupae 3d postWPP    7 pupae 4d postWPP    11 adult male 01day    10 adult male 05day    12 adult male 30day    12 adult female 01day    11 adult female 05day    13 adult female 30day    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    16 embryo 02-04hr    9 embryo 04-06hr    9 embryo 06-08hr    11 embryo 08-10hr    11 embryo 10-12hr    14 embryo 12-14hr    19 embryo 14-16hr    22 embryo 16-18hr  28 embryo 18-20hr  (36) embryo 20-22hr  31 embryo 22-24hr  32 larva L1    13 larva L2    10 larva L3 12hr old    7 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    21 white prepupae new    25 white prepupae 12hr  26 white prepupae 24hr    13 pupae 2d postWPP    13 pupae 3d postWPP    7 pupae 4d postWPP    11 adult male 01day    10 adult male 05day    12 adult male 30day    12 adult female 01day    11 adult female 05day    13 adult female 30day    13 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    16 embryo 02-04hr    9 embryo 04-06hr    9 embryo 06-08hr    11 embryo 08-10hr    11 embryo 10-12hr    14 embryo 12-14hr    19 embryo 14-16hr    22 embryo 16-18hr    28 embryo 18-20hr    36 embryo 20-22hr    31 embryo 22-24hr    32 larva L1    13 larva L2    10 larva L3 12hr old    7 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    21 white prepupae new    25 white prepupae 12hr    26 white prepupae 24hr    13 pupae 2d postWPP    13 pupae 3d postWPP    7 pupae 4d postWPP    11 adult male 01day    10 adult male 05day    12 adult male 30day    12 adult female 01day    11 adult female 05day    13 adult female 30day    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    16 embryo 02-04hr    9 embryo 04-06hr    9 embryo 06-08hr    11 embryo 08-10hr    11 embryo 10-12hr    14 embryo 12-14hr    19 embryo 14-16hr    22 embryo 16-18hr    28 embryo 18-20hr    36 embryo 20-22hr    31 embryo 22-24hr    32 larva L1    13 larva L2    10 larva L3 12hr old    7 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    12 larva L3 puffstage 7-9    21 white prepupae new    25 white prepupae 12hr    26 white prepupae 24hr    13 pupae 2d postWPP    13 pupae 3d postWPP    7 pupae 4d postWPP    11 adult male 01day    10 adult male 05day    12 adult male 30day    12 adult female 01day    11 adult female 05day    13 adult female 30day    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    67 Schneider line 2 Sg4    33 embryonic 1182-4H    45 embryonic GM2    144 embryonic Kc167    84 embryonic S1    66 embryonic S3    37 leg disc CME L1    58 wing disc CME-W2    77 wing disc ML-DmD8    51 wing disc ML-DmD9    65 wing disc ML-DmD16-c3    53 wing disc ML-DmD21    49 wing disc ML-DmD32    77 haltere disc ML-DmD17-c3    102 eye-antennal disc ML-DmD11    48 antennal disc ML-DmD20-c5    25 mixed discs ML-DmD4-c1    36 CNS ML-DmBG1-c1    50 CNS ML-DmBG2-c2    28 tumorous blood cells mbn2    127 ovary fGS/OSS    83 ovary OSC    58 ovary OSS    59 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (67) Schneider line 2 Sg4  (33) embryonic 1182-4H  (45) embryonic GM2  (144) embryonic Kc167  (84) embryonic S1  (66) embryonic S3  (37) leg disc CME L1  (58) wing disc CME-W2  (77) wing disc ML-DmD8  (51) wing disc ML-DmD9  (65) wing disc ML-DmD16-c3  (53) wing disc ML-DmD21  (49) wing disc ML-DmD32  (77) haltere disc ML-DmD17-c3  (102) eye-antennal disc ML-DmD11  (48) antennal disc ML-DmD20-c5    25 mixed discs ML-DmD4-c1  (36) CNS ML-DmBG1-c1  (50) CNS ML-DmBG2-c2  (28) tumorous blood cells mbn2  (127) ovary fGS/OSS  (83) ovary OSC  (58) ovary OSS  (59) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    67 Schneider line 2 Sg4    33 embryonic 1182-4H    45 embryonic GM2  (144) embryonic Kc167    84 embryonic S1    66 embryonic S3    37 leg disc CME L1    58 wing disc CME-W2    77 wing disc ML-DmD8    51 wing disc ML-DmD9    65 wing disc ML-DmD16-c3    53 wing disc ML-DmD21    49 wing disc ML-DmD32    77 haltere disc ML-DmD17-c3  102 eye-antennal disc ML-DmD11    48 antennal disc ML-DmD20-c5    25 mixed discs ML-DmD4-c1    36 CNS ML-DmBG1-c1    50 CNS ML-DmBG2-c2    28 tumorous blood cells mbn2  (127) ovary fGS/OSS    83 ovary OSC    58 ovary OSS    59 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    67 Schneider line 2 Sg4    33 embryonic 1182-4H    45 embryonic GM2    144 embryonic Kc167    84 embryonic S1    66 embryonic S3    37 leg disc CME L1    58 wing disc CME-W2    77 wing disc ML-DmD8    51 wing disc ML-DmD9    65 wing disc ML-DmD16-c3    53 wing disc ML-DmD21    49 wing disc ML-DmD32    77 haltere disc ML-DmD17-c3    102 eye-antennal disc ML-DmD11    48 antennal disc ML-DmD20-c5    25 mixed discs ML-DmD4-c1    36 CNS ML-DmBG1-c1    50 CNS ML-DmBG2-c2    28 tumorous blood cells mbn2    127 ovary fGS/OSS    83 ovary OSC    58 ovary OSS    59 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    67 Schneider line 2 Sg4    33 embryonic 1182-4H    45 embryonic GM2    144 embryonic Kc167    84 embryonic S1    66 embryonic S3    37 leg disc CME L1    58 wing disc CME-W2    77 wing disc ML-DmD8    51 wing disc ML-DmD9    65 wing disc ML-DmD16-c3    53 wing disc ML-DmD21    49 wing disc ML-DmD32    77 haltere disc ML-DmD17-c3    102 eye-antennal disc ML-DmD11    48 antennal disc ML-DmD20-c5    25 mixed discs ML-DmD4-c1    36 CNS ML-DmBG1-c1    50 CNS ML-DmBG2-c2    28 tumorous blood cells mbn2    127 ovary fGS/OSS    83 ovary OSC    58 ovary OSS    59 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (67) Schneider line 2 Sg4  (33) embryonic 1182-4H  (45) embryonic GM2  (144) embryonic Kc167  (84) embryonic S1  (66) embryonic S3  (37) leg disc CME L1  (58) wing disc CME-W2  (77) wing disc ML-DmD8  (51) wing disc ML-DmD9  (65) wing disc ML-DmD16-c3  (53) wing disc ML-DmD21  (49) wing disc ML-DmD32  (77) haltere disc ML-DmD17-c3  (102) eye-antennal disc ML-DmD11  (48) antennal disc ML-DmD20-c5    25 mixed discs ML-DmD4-c1  (36) CNS ML-DmBG1-c1  (50) CNS ML-DmBG2-c2  28 tumorous blood cells mbn2  (127) ovary fGS/OSS  (83) ovary OSC  (58) ovary OSS  (59) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    67 Schneider line 2 Sg4    33 embryonic 1182-4H    45 embryonic GM2  (144) embryonic Kc167    84 embryonic S1    66 embryonic S3    37 leg disc CME L1    58 wing disc CME-W2    77 wing disc ML-DmD8    51 wing disc ML-DmD9    65 wing disc ML-DmD16-c3    53 wing disc ML-DmD21    49 wing disc ML-DmD32    77 haltere disc ML-DmD17-c3  102 eye-antennal disc ML-DmD11    48 antennal disc ML-DmD20-c5    25 mixed discs ML-DmD4-c1    36 CNS ML-DmBG1-c1    50 CNS ML-DmBG2-c2    28 tumorous blood cells mbn2  127 ovary fGS/OSS    83 ovary OSC    58 ovary OSS    59 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    67 Schneider line 2 Sg4    33 embryonic 1182-4H    45 embryonic GM2    144 embryonic Kc167    84 embryonic S1    66 embryonic S3    37 leg disc CME L1    58 wing disc CME-W2    77 wing disc ML-DmD8    51 wing disc ML-DmD9    65 wing disc ML-DmD16-c3    53 wing disc ML-DmD21    49 wing disc ML-DmD32    77 haltere disc ML-DmD17-c3    102 eye-antennal disc ML-DmD11    48 antennal disc ML-DmD20-c5    25 mixed discs ML-DmD4-c1    36 CNS ML-DmBG1-c1    50 CNS ML-DmBG2-c2    28 tumorous blood cells mbn2    127 ovary fGS/OSS    83 ovary OSC    58 ovary OSS    59 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    22 cold shock, 4-day adult    16 heat shock, 4-day adult    25 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    29 Cadmium 100 mM 48 hrs, 4-day adult    67 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult    154 Zinc 5 mM 12 hrs, larvae L3    14 Zinc 4.5 mM 48 hrs, 4-day adult    93 Ethanol 2.5% 3 hrs, larvae L3    33 Ethanol 5% 3 hrs, larvae L3    21 Ethanol 10% 3 hrs, larvae L3    15 Caffeine 1.5 mg/ml 4 hrs, larvae L3    22 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    44 Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult    33 Paraquat 10 mM 48 hrs, 4-day adult    34 Rotenone 2 μg 12 hrs, larvae L3    16 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    22 cold shock, 4-day adult    16 heat shock, 4-day adult    25 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult  (29) Cadmium 100 mM 48 hrs, 4-day adult  (67) Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult  (154) Zinc 5 mM 12 hrs, larvae L3    14 Zinc 4.5 mM 48 hrs, 4-day adult  (93) Ethanol 2.5% 3 hrs, larvae L3  (33) Ethanol 5% 3 hrs, larvae L3    21 Ethanol 10% 3 hrs, larvae L3    15 Caffeine 1.5 mg/ml 4 hrs, larvae L3    22 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (44) Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult  (33) Paraquat 10 mM 48 hrs, 4-day adult  (34) Rotenone 2 μg 12 hrs, larvae L3    16 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    22 cold shock, 4-day adult    16 heat shock, 4-day adult    25 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    29 Cadmium 100 mM 48 hrs, 4-day adult    67 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult  (154) Zinc 5 mM 12 hrs, larvae L3    14 Zinc 4.5 mM 48 hrs, 4-day adult    93 Ethanol 2.5% 3 hrs, larvae L3    33 Ethanol 5% 3 hrs, larvae L3    21 Ethanol 10% 3 hrs, larvae L3    15 Caffeine 1.5 mg/ml 4 hrs, larvae L3    22 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    44 Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult    33 Paraquat 10 mM 48 hrs, 4-day adult    34 Rotenone 2 μg 12 hrs, larvae L3    16 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    22 cold shock, 4-day adult    16 heat shock, 4-day adult    25 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    29 Cadmium 100 mM 48 hrs, 4-day adult    67 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult    154 Zinc 5 mM 12 hrs, larvae L3    14 Zinc 4.5 mM 48 hrs, 4-day adult    93 Ethanol 2.5% 3 hrs, larvae L3    33 Ethanol 5% 3 hrs, larvae L3    21 Ethanol 10% 3 hrs, larvae L3    15 Caffeine 1.5 mg/ml 4 hrs, larvae L3    22 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    44 Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult    33 Paraquat 10 mM 48 hrs, 4-day adult    34 Rotenone 2 μg 12 hrs, larvae L3    16 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    22 cold shock, 4-day adult    16 heat shock, 4-day adult    25 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    29 Cadmium 100 mM 48 hrs, 4-day adult    67 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult    154 Zinc 5 mM 12 hrs, larvae L3    14 Zinc 4.5 mM 48 hrs, 4-day adult    93 Ethanol 2.5% 3 hrs, larvae L3    33 Ethanol 5% 3 hrs, larvae L3    21 Ethanol 10% 3 hrs, larvae L3    15 Caffeine 1.5 mg/ml 4 hrs, larvae L3    22 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    44 Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult    33 Paraquat 10 mM 48 hrs, 4-day adult    34 Rotenone 2 μg 12 hrs, larvae L3    16 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    22 cold shock, 4-day adult    16 heat shock, 4-day adult    25 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult  29 Cadmium 100 mM 48 hrs, 4-day adult  (67) Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult  (154) Zinc 5 mM 12 hrs, larvae L3    14 Zinc 4.5 mM 48 hrs, 4-day adult  (93) Ethanol 2.5% 3 hrs, larvae L3  (33) Ethanol 5% 3 hrs, larvae L3    21 Ethanol 10% 3 hrs, larvae L3    15 Caffeine 1.5 mg/ml 4 hrs, larvae L3    22 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (44) Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult  (33) Paraquat 10 mM 48 hrs, 4-day adult  (34) Rotenone 2 μg 12 hrs, larvae L3    16 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    22 cold shock, 4-day adult    16 heat shock, 4-day adult    25 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    29 Cadmium 100 mM 48 hrs, 4-day adult    67 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult  (154) Zinc 5 mM 12 hrs, larvae L3    14 Zinc 4.5 mM 48 hrs, 4-day adult    93 Ethanol 2.5% 3 hrs, larvae L3    33 Ethanol 5% 3 hrs, larvae L3    21 Ethanol 10% 3 hrs, larvae L3    15 Caffeine 1.5 mg/ml 4 hrs, larvae L3    22 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    44 Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult    33 Paraquat 10 mM 48 hrs, 4-day adult    34 Rotenone 2 μg 12 hrs, larvae L3    16 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    22 cold shock, 4-day adult    16 heat shock, 4-day adult    25 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    29 Cadmium 100 mM 48 hrs, 4-day adult    67 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult    154 Zinc 5 mM 12 hrs, larvae L3    14 Zinc 4.5 mM 48 hrs, 4-day adult    93 Ethanol 2.5% 3 hrs, larvae L3    33 Ethanol 5% 3 hrs, larvae L3    21 Ethanol 10% 3 hrs, larvae L3    15 Caffeine 1.5 mg/ml 4 hrs, larvae L3    22 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    44 Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult    33 Paraquat 10 mM 48 hrs, 4-day adult    34 Rotenone 2 μg 12 hrs, larvae L3    16 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_09 mE1_20_mRNA_expression_cluster_09
External Data & Images
Linkouts	BDGP expression data • SD08157 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0243512
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele cell lethal | somatic clone puc1 pucA251.1F3 lethal puc1 puc2 puc3 lethal | dominant | embryonic stage pucA251.1F3 lethal | embryonic stage | recessive puc2 pucE69 pucS023803 pucS070806 pucS135108 lethal | pupal stage, with Scer\GAL4pnr-MD237 pucGD1515 lethal | pupal stage | recessive pucE69-F lethal | recessive puc320c pucA251.1F3 pucB48 pucB48R23 pucE69 pucE69-C pucE69-E pucE69-S pucH246 pucj4E1 pucS023803 pucS070806 pucS135108 lethal | somatic clone pucE69 partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237 pucGD1515 partially lethal - majority live | recessive pucE69-F pucE69-M pucE69-Q viable pucE69-A pucE69-G pucE69-H pucE69-I pucE69-K pucEY09772 viable, with Scer\GAL4en-e16E pucDead.Scer\UAS.T:Hsap\MYC Sterility Allele female semi-sterile | germline clone pucA251.1F3 female sterile | germline clone puc1 fertile pucE69 pucEY09772 sterile | recessive pucE69-A pucE69-G pucE69-H pucE69-I pucE69-K Other Phenotypes Allele chemical resistant | dominant pucA251.1F3 pucE69 developmental rate defective | embryonic stage pucA251.1F3 increased cell death pucA251.1F3 long lived | dominant pucA251.1F3 pucE69 neuroanatomy defective, with Scer\GAL4elav-C155 pucScer\UAS.cMa neuroanatomy defective, with Scer\GAL4GMR.PF pucScer\UAS.cMa neurophysiology defective, with Scer\GAL4elav-C155 pucScer\UAS.cMa planar polarity defective, with Scer\GAL4hs.2sev pucScer\UAS.cMa radiation resistant, with Scer\GAL4en-e16E pucScer\UAS.P\T.T:Hsap\MYC stress response defective | dominant pucE69 visible, with Scer\GAL4455.2 pucScer\UAS.cMa visible, with Scer\GAL4Abd-B-LDN pucScer\UAS.cMa visible, with Scer\GAL4ap-md544 pucScer\UAS.cMa visible, with Scer\GAL4en-e16E pucScer\UAS.cMa visible, with Scer\GAL4hs.2sev pucScer\UAS.cMa visible, with Scer\GAL4opa pucEP3194 visible, with Scer\GAL4pnr-MD237 pucScer\UAS.cMa visible, with Scer\GAL4ptc-559.1 pucScer\UAS.cMa wild-type pucE69-A wild-type, with Scer\GAL4nub-AC-62 pucScer\UAS.cMa wound healing defective, with Scer\GAL4en-e16E pucScer\UAS.cMa Phenotype manifest in Allele adult external abdomen | dorsal, with Scer\GAL4dpp.blk1 pucScer\UAS.cMa adult external abdomen | dorsal, with Scer\GAL4en-e16E pucScer\UAS.cMa adult thorax, with Scer\GAL4ap-md544 pucScer\UAS.cMa adult thorax, with Scer\GAL4LE pucScer\UAS.cMa adult thorax, with Scer\GAL4opa pucEP3194 adult thorax, with Scer\GAL4pnr-MD237 pucScer\UAS.cMa anterior embryonic/larval midgut pucE69 pucGS16811 anterior embryonic/larval midgut (with Df(3R)dsx10D) pucGS16811 anterior embryonic/larval midgut | heat sensitive, with Scer\GAL4hs.PU pucScer\UAS.cMa bouton, with Scer\GAL4elav-C155 pucScer\UAS.cMa cardioblast pucE69 chorion | maternal effect | somatic clone pucA251.1F3 collar | maternal effect | somatic clone pucA251.1F3 denticle belt pucA251.1F3 denticle belt | maternal effect pucA251.1F3 dorsal appendage, with Scer\GAL455B pucScer\UAS.cMa dorsal appendage, with Scer\GAL4CY2 pucScer\UAS.cMa dorsal appendage, with Scer\GAL4hs.PB pucScer\UAS.cMa dorsal appendage, with Scer\GAL4T155 pucScer\UAS.cMa dorsal appendage | maternal effect | somatic clone pucA251.1F3 dorsal fine hair pucE69 dorsal hair pucA251.1F3 dorsal mesothoracic disc & peripodial epithelium & prepupa, with Scer\GAL4Act5C.PI pucScer\UAS.cMa dorsal mesothoracic disc & peripodial epithelium & prepupa, with Scer\GAL4Act5C.PI, pucE69 pucScer\UAS.cMa dorsal mesothoracic disc & peripodial epithelium & prepupa, with Scer\GAL4Act5C.PI, pucScer\UAS.cMa pucE69 dorsal mesothoracic disc & peripodial stalk & prepupa, with Scer\GAL4Act5C.PI pucScer\UAS.cMa dorsal mesothoracic disc & peripodial stalk & prepupa, with Scer\GAL4Act5C.PI, pucE69 pucScer\UAS.cMa dorsal mesothoracic disc & peripodial stalk & prepupa, with Scer\GAL4Act5C.PI, pucScer\UAS.cMa pucE69 egg, with Scer\GAL4hs.PB pucScer\UAS.cMa egg | maternal effect | somatic clone pucA251.1F3 egg | somatic clone, with Scer\GAL4Act5C.PP pucScer\UAS.cMa egg operculum | maternal effect | somatic clone pucA251.1F3 ejaculatory bulb, with Scer\GAL4dpp.blk1 pucScer\UAS.cMa ejaculatory bulb, with Scer\GAL4en-e16E pucScer\UAS.cMa ejaculatory duct, with Scer\GAL4dpp.blk1 pucScer\UAS.cMa ejaculatory duct, with Scer\GAL4en-e16E pucScer\UAS.cMa embryo | dorsal, with Scer\GAL469B pucScer\UAS.cMa embryo | dorsal, with Scer\GAL4e22c pucScer\UAS.cMa embryonic/first instar larval cuticle pucE69 embryonic/first instar larval cuticle, with Scer\GAL469B pucScer\UAS.cMa embryonic/first instar larval cuticle | dorsal pucA251.1F3 pucE69 embryonic/first instar larval cuticle | dorsal, with Scer\GAL4hs.PB pucScer\UAS.cMa embryonic/first instar larval cuticle | maternal effect pucA251.1F3 embryonic/larval dorsal vessel | posterior pucS023803 pucS070806 pucS135108 embryonic/larval proventriculus pucGS16811 embryonic dorsal epidermis puc2 pucE69 embryonic dorsal vessel pucE69 embryonic epidermis pucE69 embryonic epidermis | dorsal pucA251.1F3 pucE69 embryonic epidermis | dorsal, with Scer\GAL4arm.PS pucScer\UAS.cMa embryonic epidermis | dorsal, with Scer\GAL4arm.T:Hsim\VP16 pucScer\UAS.cMa embryonic leading edge cell pucE69 embryonic leading edge cell, with Scer\GAL4ptc-559.1 pucScer\UAS.cMa embryonic segment | dorsal puc2 embryonic segment | maternal effect pucA251.1F3 eye puc1 pucA251.1F3 eye, with Scer\GAL4GMR.PF pucScer\UAS.cMa eye, with Scer\GAL4hs.2sev pucScer\UAS.cMa eye disc | somatic clone pucE69 eye photoreceptor cell, with Scer\GAL4GMR.PF pucScer\UAS.cMa follicle cell | somatic clone pucA251.1F3 genital arch, with Scer\GAL4en-e16E pucScer\UAS.cMa genital arch, with Scer\GAL4ptc-559.1 pucScer\UAS.cMa germline cell | embryonic stage 15 pucE69 imaginal disc pucE69-F pucE69-M pucE69-Q lamina, with Scer\GAL4GMR.PF pucScer\UAS.cMa macrochaeta | supernumerary, with Scer\GAL4ptc-559.1 pucScer\UAS.P\T.T:Hsap\MYC male genitalia, with Scer\GAL4en-e16E pucScer\UAS.P\T.T:Hsap\MYC male terminalia, with Scer\GAL4Abd-B-LDN pucScer\UAS.cMa male terminalia, with Scer\GAL4dpp.blk1 pucScer\UAS.cMa male terminalia, with Scer\GAL4en-e16E pucScer\UAS.cMa male terminalia, with Scer\GAL4ptc-559.1 pucScer\UAS.cMa mesothoracic cleft | ectopic, with Scer\GAL4ap-md544 pucScer\UAS.cMa mesothoracic tergum, with Scer\GAL4pnr-MD237 pucScer\UAS.cMa micropyle, with Scer\GAL455B pucScer\UAS.cMa micropyle, with Scer\GAL4CY2 pucScer\UAS.cMa micropyle, with Scer\GAL4slbo.2.6 pucScer\UAS.cMa micropyle, with Scer\GAL4T155 pucScer\UAS.cMa neuromuscular junction | larval stage, with Scer\GAL4elav-C155 pucScer\UAS.cMa neuron, with Scer\GAL4GMR.PF pucScer\UAS.cMa nucleus & follicle cell | somatic clone pucA251.1F3 nurse cell | maternal effect | somatic clone pucA251.1F3 nurse cell | somatic clone, with Scer\GAL4Act5C.PP pucScer\UAS.cMa ommatidium, with Scer\GAL4hs.2sev pucScer\UAS.cMa peripheral nervous system pucH246 presumptive embryonic/larval peripheral nervous system pucS023803 pucS070806 pucS135108 scutellar bristle | ectopic, with Scer\GAL4455.2 pucScer\UAS.cMa scutellum, with Scer\GAL4455.2 pucScer\UAS.cMa ventral midline | dorsal pucE69 wing disc, with Scer\GAL4Bx-MS1096-KE pucScer\UAS.cMa wing disc, with Scer\GAL4en-e16E pucScer\UAS.cMa wing disc | prepupal stage, with Scer\GAL4Act5C.PI pucScer\UAS.cMa wing disc | prepupal stage, with Scer\GAL4Act5C.PI, pucE69 pucScer\UAS.cMa wing disc | prepupal stage, with Scer\GAL4Act5C.PI, pucScer\UAS.cMa pucE69
Classical Alleles ( 50 )
For All Classical Alleles Show
Allele of puc Class Mutagen Stocks Known lesion puc1 1 -- puc67BDn 1 -- pucA251.1F3 P-element activity 1 Yes pucc03339 piggyBac transposase 1 -- pucc04318 piggyBac transposase 1 -- pucc05229 piggyBac transposase 1 -- pucc06751 piggyBac transposase 1 -- pucd08940 P-element activity 1 -- pucd11271 P-element activity 1 -- puce01329 piggyBac transposase 1 -- puce01459 1 -- pucE69 loss of function allele P-element activity 1 Yes pucEY09772 P-element activity 1 -- pucf02859 piggyBac transposase 1 -- pucf03088 1 -- pucf04998 piggyBac transposase 1 -- pucf06210 piggyBac transposase 1 -- pucGAL4E69 Delta2-3 gene conversion 1 Yes pucGS16811 1 -- pucH246 loss of function allele ethyl methanesulfonate 1 -- pucJK0 Delta2-3 1 Yes pucNP1311 1 -- puc0694 P-element activity 0 -- puc2 0 -- puc320c P-element activity 0 Yes puc3 0 -- pucB48 P-element activity 0 Yes pucB48R23 0 Yes pucd11548 P-element activity 0 -- pucDG06708 0 -- pucE69-A gene conversion 0 -- pucE69-C gene conversion 0 -- pucE69-E gene conversion 0 -- pucE69-F gene conversion 0 -- pucE69-G gene conversion 0 -- pucE69-H gene conversion 0 -- pucE69-I gene conversion 0 -- pucE69-K gene conversion 0 -- pucE69-M gene conversion 0 -- pucE69-Q gene conversion 0 -- pucE69-S gene conversion 0 -- pucEP1225 P-element activity 0 Yes pucEP3194 0 -- pucj4E1 P-element activity 0 Yes pucKG04275 P-element activity 0 -- pucPBac3929 piggyBac transposase 0 Yes pucS023803 Delta2-3 P-element activity 0 Yes pucS070806 P-element activity 0 -- pucS135108 Delta2-3 P-element activity 0 -- pucWG1173 P-element activity 0 --
Alleles Carried on Transgenic Constructs ( 9 )
For All Alleles Carried on Transgenic Constructs Show
Allele of puc Class Mutagen Stocks Known lesion pucGD1515 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes pucGL00504 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes pucHMS01386 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes pucJF01127 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes pucDead.Scer\UAS.T:Hsap\MYC in vitro construct - amino acid replacement 0 Yes pucdsRNA.cBa in vitro construct - RNAi 0 Yes pucScer\UAS.cMa in vitro construct - regulatory fusion 0 Yes pucScer\UAS.P\T.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes pucT:SV5\V5 in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)BSC465 (Christensen et al., 2008.4.15) Df(3R)BSC466 (Christensen et al., 2008.4.15) Df(3R)CA1 (Salzberg et al., 1994) Df(3R)D6 (Baker et al., 1991) Df(3R)D7 (Baker et al., 1991) Df(3R)dsx2D (Baker et al., 1991) Df(3R)dsx3 (Baker et al., 1991, Ring and Martinez Arias, 1993) Df(3R)dsx10M (Baker et al., 1991, Ring and Martinez Arias, 1993, Salzberg et al., 1994) Df(3R)dsx11 (Baker et al., 1991) Df(3R)dsx29 (Baker et al., 1991, Salzberg et al., 1994) Df(3R)p40 (Baker et al., 1991, Ring and Martinez Arias, 1993) Df(3R)p712 (Pastor-Pareja et al., 2004, Spradling et al., 1999, BDGP Project Members, 1994-1999)
Not disrupted in	Df(3L)AC1 (Kirchner et al., 2007) Df(3R)Antp17 (Baker et al., 1991) Df(3R)dsx2M (Baker et al., 1991) Df(3R)dsx15 (Baker et al., 1991, Ring and Martinez Arias, 1993) Df(3R)dsx21 (Baker et al., 1991, Ring and Martinez Arias, 1993) Df(3R)dsx43 (Baker et al., 1991) Df(3R)p13 (Baker et al., 1991, Ring and Martinez Arias, 1993) Df(3R)p30 (Baker et al., 1991) Df(3R)p-XT103 (Salzberg et al., 1994)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD1515} NA P{TRiP.GL00504} NA P{TRiP.HMS01386} NA P{TRiP.JF01127} NA P{UASp-puc.MYC} NA P{UAS-puc.Dead.MYC} NA P{UAS-puc.M} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{A92}pucE69 No P{lacW}puc320c No P{lacW}pucj4E1 No P{lacW}pucS023803 No P{lacW}pucS135108 No P{lacW}pucWG1173 No P{lacZ}puc0694 No P{lacZ}pucB48 No P{lArB}pucA251.1F3 No insertion of mobile activating element P{EP}pucEP1225 NA P{EP}pucEP3194 NA P{EPgy2}pucEY09772 NA P{GSV6}pucGS16811 NA P{XP}pucd08940 NA P{XP}pucd11271 NA P{XP}pucd11548 NA miscellaneous insertions P{GAL4E69}pucGAL4E69 NA P{SUPor-P}pucKG04275 NA P{wHy}pucDG06708 NA PBac{IRES-GFP.GT}pucPBac3929 NA PBac{PB}pucc03339 NA PBac{PB}pucc04318 NA PBac{PB}pucc05229 NA PBac{PB}pucc06751 NA PBac{RB}puce01329 NA PBac{RB}puce01459 NA PBac{WH}pucf02859 NA PBac{WH}pucf03088 NA PBac{WH}pucf04998 NA PBac{WH}pucf06210 NA insertion of enhancer trap binary system P{GawB}pucE69-A No P{GawB}pucE69-C No P{GawB}pucE69-E No P{GawB}pucE69-F No P{GawB}pucE69-G No P{GawB}pucE69-H No P{GawB}pucE69-I No P{GawB}pucE69-K No P{GawB}pucE69-M No P{GawB}pucE69-Q No P{GawB}pucE69-S No P{GawB}pucNP1311 No
Gene Ontology: Function, Process & Cellular Component ( 37 unique terms )
Terms Based on Experimental Evidence ( 28 terms )
Molecular Function
CV term Evidence References
JUN kinase phosphatase activity inferred from mutant phenotype (Martin-Blanco et al., 1998) phosphoprotein phosphatase activity inferred from direct assay (Martin-Blanco et al., 1998)
Biological Process
CV term Evidence References
actin cytoskeleton organization inferred from mutant phenotype (Martin-Blanco et al., 1998) actin filament organization inferred from mutant phenotype (Kiger et al., 2003) border follicle cell migration inferred from genetic interaction with Pvr (Mathieu et al., 2007) chitin-based embryonic cuticle biosynthetic process inferred from mutant phenotype (McEwen and Peifer, 2005) compound eye development inferred from mutant phenotype (McEwen and Peifer, 2005) determination of adult lifespan inferred from mutant phenotype (Wang et al., 2003) determination of digestive tract left/right asymmetry inferred from mutant phenotype (Taniguchi et al., 2007) dorsal appendage formation inferred from mutant phenotype (Suzanne et al., 2001) dorsal closure inferred from mutant phenotype (Ring and Martinez Arias, 1993) embryonic anterior midgut (ectodermal) morphogenesis inferred from mutant phenotype (Taniguchi et al., 2007) epidermis development inferred from mutant phenotype (Ring and Martinez Arias, 1993) imaginal disc eversion inferred from mutant phenotype (Pastor-Pareja et al., 2004) imaginal disc fusion inferred from mutant phenotype (Pastor-Pareja et al., 2004) JNK cascade inferred from mutant phenotype (Martin-Blanco et al., 1998) micropyle formation inferred from mutant phenotype (Suzanne et al., 2001) negative regulation of apoptotic process inferred from mutant phenotype (McEwen and Peifer, 2005) negative regulation of stress-activated protein kinase signaling cascade inferred from mutant phenotype (Boutros et al., 2002) ovarian follicle cell development inferred from mutant phenotype (Jordan et al., 2006) protein dephosphorylation inferred from direct assay AND inferred from sequence or structural similarity (Martin-Blanco et al., 1998) protein secretion inferred from mutant phenotype (Bard et al., 2006) regulation of cell shape inferred from mutant phenotype (Kiger et al., 2003) regulation of G2/M transition of mitotic cell cycle inferred from mutant phenotype (Chen et al., 2007) response to oxidative stress inferred from mutant phenotype (Wang et al., 2003) wound healing inferred from direct assay (Manfruelli et al., 2002) inferred from expression pattern (Ramet et al., 2002) inferred from mutant phenotype (Bosch et al., 2005)
Cellular Component
CV term Evidence References
endoplasmic reticulum inferred from direct assay (Bard et al., 2006) Golgi apparatus inferred from direct assay (Bard et al., 2006)
Terms Based on Predictions or Assertions ( 14 terms )
Molecular Function
CV term Evidence References
JUN kinase phosphatase activity non-traceable author statement (Martin-Blanco, 1998.1.12) traceable author statement (Prokopenko et al., 2000, Harden, 2002) MAP kinase tyrosine/serine/threonine phosphatase activity inferred from biological aspect of ancestor with PANTHER:PTN000016984 (assigned by RefGenome) (Gaudet et al., 2010) non-traceable author statement (Riesgo-Escovar et al., 1996, Noselli and Agnes, 1999) traceable author statement (Van Aelst and Symons, 2002, Harden, 2002) protein tyrosine/serine/threonine phosphatase activity inferred from sequence or structural similarity (Martin-Blanco et al., 1998) non-traceable author statement (Morrison et al., 2000) protein tyrosine phosphatase activity inferred from electronic annotation with InterPro:IPR016130 (FlyBase Curators et al., 2004-) inferred from sequence or structural similarity (Martin-Blanco et al., 1998)
Biological Process
CV term Evidence References
dorsal closure non-traceable author statement (Van Aelst and Symons, 2002) traceable author statement (Prokopenko et al., 2000, Noselli and Agnes, 1999, Lamka and Lipshitz, 1999, Harden, 2002) establishment of planar polarity non-traceable author statement (Mlodzik, 2002) inactivation of MAPK activity inferred from biological aspect of ancestor with PANTHER:PTN000016984 (assigned by RefGenome) (Gaudet et al., 2010) JNK cascade non-traceable author statement (Riesgo-Escovar et al., 1996, Noselli and Agnes, 1999) traceable author statement (Prokopenko et al., 2000) negative regulation of JNK cascade non-traceable author statement (Gibson and Schubiger, 2001) traceable author statement (Stronach and Perrimon, 2002, Van Aelst and Symons, 2002, Harden, 2002) ommatidial rotation non-traceable author statement (Van Aelst and Symons, 2002) ovarian follicle cell development traceable author statement (Dobens and Raftery, 2000) peripheral nervous system development traceable author statement (Salzberg et al., 1994, Prokopenko et al., 2000) protein dephosphorylation inferred from biological aspect of ancestor with PANTHER:PTN000016642 (assigned by RefGenome) (Gaudet et al., 2010) non-traceable author statement (Martin-Blanco, 1998.1.12, Morrison et al., 2000) suture of dorsal opening non-traceable author statement (Riesgo-Escovar et al., 1996)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	gene   nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
puc - Atx2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) puc - B52 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) puc - CG13321 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) puc - CG13472 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) puc - CG1458 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) puc - CG4199 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) puc - CG4611 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) puc - CG6701 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) puc - CtBP anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) puc - Gp210 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) puc - Jupiter anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) puc - Plod anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) puc - RpL35A anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) puc - RpS12 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) puc - Sec23 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) puc - Srp72 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) puc - Top3β anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) puc - Zn72D anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) puc - cathD anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) puc - hyd anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) puc - l(3)72Ab anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) puc - larp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) puc - mRpL39 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) puc - nito anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) puc - p38b anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) puc - qkr58E-3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) puc - ref(2)P anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) puc - tyf anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions puc allele Gene References puc1 arm (McEwen et al., 2000) pucA251.1F3 arm (McEwen et al., 2000, Cox et al., 2000) hep (Martin-Blanco et al., 1998) pan (McEwen et al., 2000) peb (Wilk et al., 2004) wg (McEwen et al., 2000) pucE69 bi (Adachi-Yamada et al., 1999) dpp (Adachi-Yamada et al., 1999) eg (Gritzan et al., 2002) hep (Martin-Blanco et al., 1998, Zeitlinger and Bohmann, 1999, Agnes et al., 1999, Weber et al., 2000, Wang et al., 2003) Jra (Sanyal et al., 2002) kay (Zeitlinger and Bohmann, 1999, Ramet et al., 2002) knrl (Gritzan et al., 2002) Pvr (Ishimaru et al., 2004) Rac1 (Stronach and Perrimon, 2002) Sac1 (Wei et al., 2003) slpr (Stronach and Perrimon, 2002) Src42A (Tateno et al., 2000) tkv (Adachi-Yamada et al., 1999) pucE69-F hep (Bosch et al., 2005) pucScer\UAS.cMa bi (del Alamo Rodriguez et al., 2004) bsk (Sanyal et al., 2002) dm (Moreno and Basler, 2004) kay (Sanyal et al., 2002) rl (Martin-Blanco, 1998) unspecified bsk (McEwen and Peifer, 2005) chm (Miotto et al., 2006) Dok (Biswas et al., 2006) flw (Kirchner et al., 2007) Fps85D (Murray et al., 2006) grim (Mueller et al., 2005) H (Mueller et al., 2005) Irc (Ha et al., 2005) L (Singh et al., 2006) N (Zecchini et al., 1999) p53 (Mueller et al., 2005) park (Cha et al., 2005) Pvr (Mathieu et al., 2007) rpr (Mueller et al., 2005) slpr (Polaski et al., 2006, Stronach and Perrimon, 1999) W (Mueller et al., 2005)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 66141 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0243512 DroID - A comprehensive database of gene and protein interactions. • FBgn0243512 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 40958
Orthologs
OrthoDB Orthologs (38) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG612MRC Dipteran inclusive ortholog search EOG60P4GD Insect inclusive ortholog search EOG6GXDH2 Arthropod inclusive ortholog search EOG6VX0MF Metazoa inclusive ortholog search EOG67Q907
Orthologs in Drosophila Species (EOG612MRC)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\puc     Drosophila simulans   Dsim\GD18513     Drosophila sechellia   Dsec\GM23703     Drosophila erecta   Dere\GG23159     Drosophila yakuba   Dyak\GE25849     Drosophila ananassae   Dana\GF16724     Drosophila pseudoobscura pseudoobscura   Dpse\GA20632     Drosophila persimilis   Dper\GL24256     Drosophila willistoni   Dwil\GK14377     Drosophila virilis   Dvir\GJ24161     Drosophila mojavensis   Dmoj\GI23305     Drosophila grimshawi   Dgri\GH18324
Orthologs in non-Drosophila Dipterans (EOG60P4GD)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL010411   Anopheles gambiae Malaria mosquito  Agam\AGAP004353   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ008018
Orthologs in non-Dipteran Insects (EOG6GXDH2)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB44634   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH17293   Atta cephalotes Leafcutter ant  Acep\ACEP13037   Camponotus floridanus Florida carpenter ant  Cflo\CFLO16978   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL18777   Linepithema humile Argentine ant  Lhum\LH22171   Pogonomyrmex barbatus Red harvester ant  Pbar\PB15639   Solenopsis invicta Red fire ant  Sinv\SINV16113   Acyrthosiphon pisum Pea aphid  Apim\ACYPI005391   Pediculus humanus Human body louse  Phum\PHUM490910   Tribolium castaneum Red flour beetle  Tcas\TC010766
Orthologs in non-Insect Arthropods (EOG6VX0MF)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_23494   Ixodes scapularis Deer tick  Isca\ISCW021412
Orthologs in non-Arthropod Metazoa (EOG67Q907)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_006513gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_023676gn   Danio rerio Zebrafish  Drer\si:dkey-175m17.7   Danio rerio Zebrafish  Drer\si:ch211-180b22.4   Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000033337   Xenopus tropicalis Western clawed frog  Xtro\dusp10.1   Gallus gallus Domestic chicken  Ggal\DUSP10   Mus musculus House mouse  Mmus\Dusp10   Rattus norvegicus Norway rat  Rnor\Dusp10   Homo sapiens Human  Hsap\DUSP10
Human Orthologs (1)
Gene OMIM HGNC Hsap\DUSP10
AAA Orthologs (0) based on analysis using Dmel annotation version 4.3
No orthologs identified
Stocks & Reagents
Stocks Listed in FlyBase ( 27 )
Harvard	- P{XP}pucd08940 - P{XP}pucd11271 - PBac{PB}pucc03339 - PBac{PB}pucc04318 - PBac{PB}pucc05229 - PBac{PB}pucc06751 - PBac{RB}puce01329 - PBac{RB}puce01459 - PBac{WH}pucf02859 - PBac{WH}pucf03088 - PBac{WH}pucf04998 - PBac{WH}pucf06210
	More stocks available...
Genomic Clones ( 2 )
	BACR07M14 BACR45A07 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 75 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	SD08157
Other clones	DPiM_SD08157
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RE04663 RE18491 RE47993 RE72934 SD11844 SD19950 SD27806
Other clones	104611 CK01.23D.E5 Dmel_neb_008034_0659_3001 EC13355 EC051594 EC052078 EC053107 EC053592 EC055128 EC055771 EC056603 EK009357 EK030022 EK034848 EK057828 EK128954 EK201060 EK201133 EK229161 EK265939 EP09860 EP10005 FGH001H05 FGH110B11 FGM002H12 RP003006231 RP003056487 RP003079405 RP003082462 RP003086493 RP003126201 RP003142400 RP003151486 RP003155312 RP003155983 RP003157479 RP003213587 RP003229982 RP003232950 RP003243110 RP003258150 RP003258681 RP003275834 RP003281524 RP003294014 RP003313555 RP003332194 RP003339932 RP003354753 RP003380280 RP003381570 RP003383614 RP003433521 RP003440605 RP003490863 RP003522965 RP003565140 RP003567314 RP003568370 RP003583889 RP003605185 RP003629030 RP003645222 RP003661119 RP003661689 RP003665258
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0243512 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 40958
Antibody Information
Other Information
Discoverer
	L. and Y. Jan.
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: puc CG7850 (Zhao, 1999.11)
Source for database merge of	Source for merge of: puc hrt (Prokopenko et al., 2000) Source for merge of: puc l(3)j4E1 (Kirchner et al., 2007)
Additional comments
Other Comments
	dsRNA directed against puc inhibits protein secretion in S2 cells, but has no apparent effect on Golgi organisation. (Bard et al., 2006) dsRNA directed against this gene has been used in a screen for genes required for constitutive protein secretion. (Bard et al., 2006) RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 cells: change from round to spindle-shaped, with the formation of F-actin puncta and microtubule extensions. Alterations of cell shape are also evident in S2R+ cells. (Kiger et al., 2003) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) Proper levels of puc activity in the follicle cells are critical for the production of a normal egg. (Dobens et al., 2001) Either reduced or increased puc activity results in incomplete nurse cell dumping and aberrant dorsal appendages. (Dobens et al., 2001) Mutations in puc, a known negative regulator of JNK signaling, suppress both the dorsal closure and ventral segment polarity defects associated with a decrease in wg signaling. (McEwen et al., 2000) puc normally antagonizes wg signalling in the ventral epidermis. (McEwen et al., 2000) Loss of function and gain of function mutants in puc cause planar polarity phenotypes in the eye. (Weber et al., 2000) hep controls puc expression but not dpp expression in imaginal discs. (Agnes et al., 1999) Candidate gene for quantitative trait (QTL) locus determining bristle number. (Nuzhdin et al., 1999) puc has a negative regulatory role in thorax closure during metamorphosis. (Zeitlinger and Bohmann, 1999) puc encodes a phosphatase that regulates signaling through the bsk pathway and participates in a negative feedback loop leading to a transient activity of the bsk signal during dorsal closure. (Martin-Blanco et al., 1998) puc is both a repressor and a target of hep function in the leading edge. The combined and antagonistic functions of hep and puc maintain appropriate levels of puc and dpp activities in migrating epithelia during dorsal closure. These results indicate a leading edge cell identity and dorsal closure depend on a balance between bsk activation and puc repression. (Glise and Noselli, 1997) puc expression is regulated by bsk function. (Riesgo-Escovar et al., 1996) puc is negatively autoregulated in the follicle cells and that depression of puc in the squamous follicle cells at stage 10 is necessary for nurse cell transport. (Dobens et al., 1995) Ectopic expression of puc can cause defects in dorsal closure, dorsal puckering and denticle belt patterning. These phenotypes overlap those caused by loss of function alleles of puc. (Gampel and Martinez Arias, 1995) The General Council of the Velcro Group Corporation prohibited the use of the name 'velcro' for this gene, as that would constitute an infringement of trademark. (Skaer, 1995.12.11) Mutation in puc affects neuronal connections, affecting fasciculation. (Salzberg et al., 1994) puc is required for PNS development in the embryo. (Salzberg et al., 1994)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079549 RefSeq (Proteins) • NP_524273 DDBJ / EMBL / GenBank • AAF54191 AAL29164 AF174657 AI542084 AJ223360 AQ025202 AQ026320 AW944549 AY061616 BH615123 CAA11282 CL610255 CZ468616 CZ469169 CZ469798 CZ470972 CZ476486 CZ477654 CZ477799 CZ479140 CZ485418 CZ487585 CZ488867 Entrez Gene - A searchable database of RefSeq genes. • 40958 UniProtKB/TrEMBL • O46122 Q9VHV8
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Protein-tyrosine/Dual specificity phosphatase (IPR000387) Dual specificity phosphatase, catalytic domain (IPR000340) Protein-tyrosine phosphatase, active site (IPR016130) Dual specificity phosphatase, subgroup, catalytic domain (IPR020422) Dual specificity phosphatase (IPR024950) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • SD08157
Linkouts
	BioGRID - A database of protein and genetic interactions • 66141 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0243512 DroID - A comprehensive database of gene and protein interactions. • FBgn0243512 DRSC - Results from RNAi screens. • FBgn0243512 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0243512 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0243512 FlyReactome - A curated knowledgebase of Drosophila melanogaster pathways • 205628 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 40958 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /torstoll/puckrd1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 40958 modMine - Data generated by the modENCODE project. • FBgn0243512
Synonyms & Secondary IDs ( 20 )
Reported As
Symbol Synonym	0238/03 (Deak et al., 1997) 1351/08 (Deak et al., 1997) CG7850 (Bard et al., 2006, De Gregorio et al., 2001, Morrison et al., 2000, Quinones-Coello, 2007, Sun et al., 2010, Carrera et al., 2008, Ni et al., 2008, Ni et al., 2010.12.1, Ni et al., 2011.7.19, Wheeler et al., 2004) hrt (Nuzhdin et al., 1999, Salzberg et al., 1997, Salzberg et al., 1994, ) JNK-DSP (Llense and Martín-Blanco, 2008) l(3)84Eh (Ring and Martinez Arias, 1993, Baker et al., 1991, ) l(3)A251.1 (Cox et al., 2000) l(3)j4E1   puc (Doheny et al., 2008, Bates et al., 2008, Hyun et al., 2006, Jordan et al., 2005, FlyBase Curators, 2008, Yoshioka et al., 2008, Pezzulo et al., 2012, Tiwari and Roy, 2009, Tripura et al., 2011, Rynes et al., 2012, Kleino et al., 2008, Neuman-Silberberg, 2007, Lennox and Stronach, 2010, Ohayon et al., 2009, Chen et al., 2007, Kirchner et al., 2007, Singh et al., 2006, Pastor-Pareja et al., 2004, Libert et al., 2007, Taniguchi et al., 2007, Murray et al., 2006, McEwen and Peifer, 2005, Delaney et al., 2006, Cha et al., 2003, Dionne et al., 2006, Baena-Lopez and Garcia-Bellido, 2006, Ryoo et al., 2004, Herz et al., 2006, Tsuda et al., 2006, Löw et al., 2007, Friedman and Perrimon, 2006, Pfleger et al., 2007, Srahna et al., 2006, Cerrato et al., 2006, Quinones-Coello, 2007, Jordan et al., 2006, Sun et al., 2010, Chatterjee and Bohmann, 2012, Uhlirova and Bohmann, 2006, Sem et al., 2002, Polaski et al., 2006, McNamee and Brodsky, 2008, Baeg et al., 2007, Christensen et al., 2008.4.15, Christensen et al., 2008.4.15, Schimmelpfeng et al., 2006, Sato et al., 2006, Krieser et al., 2007, Avet-Rochex et al., 2007, Wang and Ward, 2010, Homsy et al., 2006, Vidal et al., 2010, Nelson et al., 2005, Balakireva et al., 2006, Singh et al., 2010, González et al., 2009, Massaro et al., 2009, Kankel et al., 2007, Thomas et al., 2009, Apidianakis et al., 2009, Opota et al., 2011, Bhuin and Roy, 2010, McNamee and Brodsky, 2009, Wu et al., 2010, Seisenbacher et al., 2011, Belacortu et al., 2011, Greenberg and Hatini, 2011, Rumpf et al., 2011, Shaw et al., 2010, Umemori et al., 2009, Bakal et al., 2008, Luo et al., 2007, Xue et al., 2007, Langton et al., 2007, Taniguchi et al., 2007, Lin et al., 2007, Umemori et al., 2007, Poernbacher et al., 2012, Lee et al., 2005, Llense and Martín-Blanco, 2008, Manjon et al., 2007, Bidla et al., 2007, Baril et al., 2009, Dichtel-Danjoy et al., 2009, Ziv et al., 2009, Rodahl et al., 2009, Jiang and Crews, 2003, Carrera et al., 2008, Hull-Thompson et al., 2009, Lee et al., 2006, Meinander et al., 2012, Tsuda et al., 2005, Ohsawa et al., 2011, Buchon et al., 2009, Wu et al., 2009, Jiang et al., 2009, Igaki et al., 2006, Igaki et al., 2006, Zeitlinger et al., 2007, Owusu-Ansah et al., 2008, Weber et al., 2008, Cruz et al., 2009, Glavic et al., 2009, Grima et al., 2008, Owusu-Ansah and Banerjee, 2009, Mallik and Lakhotia, 2009, Tuxworth et al., 2009, Wang et al., 2010, Rousset et al., 2010, Campos et al., 2010, Gettings et al., 2010, Sorrosal et al., 2010, Li et al., 2010, Xi et al., 2008, Beam and Moberg, 2010, Warner et al., 2010, Tamori et al., 2010, Okumura et al., 2010, Neisch et al., 2010, Yavari et al., 2010, Bergantiños et al., 2010, Xiong et al., 2010, Chen et al., 2010, Mattila et al., 2005, Bosch et al., 2008, Shen et al., 2009, Padash-Barmchi et al., 2010, Bosch et al., 2010, Marinho et al., 2011, Lam et al., 2010, Portela et al., 2010, Boyd et al., 2011, Resnik-Docampo and de Celis, 2011, Jiang et al., 2011, Pastor-Pareja et al., 2008, Glittenberg et al., 2011, Jiang et al., 2011, Coffman et al., 2005, Boutros et al., 2002, Thevenon et al., 2009, Umehara et al., 2010, Maillet et al., 2008, Biteau et al., 2008, Libert et al., 2008, Wheeler et al., 2004, Nam et al., 2012, Abruzzi et al., 2011, Colombani et al., 2012, Gesellchen et al., 2005, Kelsey et al., 2012, Gowda et al., 2012, Lim et al., 2012, Bangi et al., 2012, Marchal et al., 2012, Jemc et al., 2012, Gonda et al., 2012, Chakrabarti et al., 2012, Lu et al., 2012) Puc (Vidal et al., 2006, Ha et al., 2009, Ha et al., 2005, Krieser et al., 2007, Kadrmas et al., 2004, Lee et al., 2007, Bond and Foley, 2009, Zhuang et al., 2009, Zhang et al., 2009, Melani et al., 2008, Liu et al., 2009, Wang et al., 2010, Wang et al., 2010, Cully et al., 2010, Benitez et al., 2010, Friedman et al., 2011) PUC (Xia and Karin, 2004, Sun et al., 2008) PUC/MKP (Morrison et al., 2000) vco (Martinez Arias, 1993, )
Name Synonym	hearty (Salzberg et al., 1994, ) Jun N-terminal kinase dual-specificity phosphatase (Llense and Martín-Blanco, 2008) lethal (3) j4E1   puckered (Doheny et al., 2008, Bates et al., 2008, Jordan et al., 2005, Yoshioka et al., 2008, Ha et al., 2009, Kleino et al., 2008, Neuman-Silberberg, 2007, Lennox and Stronach, 2010, Ohayon et al., 2009, Martin-Blanco, 1998.1.12, Taniguchi et al., 2007, Cha et al., 2003, Baena-Lopez and Garcia-Bellido, 2006, Tsuda et al., 2006, Cerrato et al., 2006, Eissenberg, 2006, Mathieu et al., 2007, Sato et al., 2006, Avet-Rochex et al., 2007, Wang and Ward, 2010, Homsy et al., 2006, Vidal et al., 2010, Nelson et al., 2005, Lee et al., 2007, Massaro et al., 2009, Kankel et al., 2007, Bakal et al., 2008, Tadros et al., 2007, Xue et al., 2007, Taniguchi et al., 2007, Lee et al., 2005, Llense and Martín-Blanco, 2008, Melani et al., 2008, Manjon et al., 2007, Dichtel-Danjoy et al., 2009, Rodahl et al., 2009, Jiang and Crews, 2003, Lee et al., 2006, Meinander et al., 2012, Tsuda et al., 2005, Buchon et al., 2009, Wu et al., 2009, Jiang et al., 2009, Shalaby et al., 2009, Owusu-Ansah et al., 2008, Wang et al., 2004, Weber et al., 2008, Grima et al., 2008, Owusu-Ansah and Banerjee, 2009, Mallik and Lakhotia, 2009, Rousset et al., 2010, Leulier et al., 2006, Campos et al., 2010, Gettings et al., 2010, Li et al., 2010, Beam and Moberg, 2010, Warner et al., 2010, Tamori et al., 2010, Okumura et al., 2010, Bergantiños et al., 2010, Xiong et al., 2010, Mattila et al., 2005, Bosch et al., 2008, Shen et al., 2009, Padash-Barmchi et al., 2010, Kauppila et al., 2003, Radyuk et al., 2010, Portela et al., 2010, Boyd et al., 2011, Jiang et al., 2011, Umehara et al., 2010, Maillet et al., 2008, Colombani et al., 2012, Bangi et al., 2012, Förster and Luschnig, 2012, Jemc et al., 2012, Lu et al., 2012, Wang et al., 2012) Puckered (Huh et al., 2007, Herz et al., 2006, Kadrmas et al., 2004, Orr et al., 2008, Balakireva et al., 2006, Zhang et al., 2009, Neisch et al., 2010, Langton et al., 2007, Kim et al., 2007, Sun et al., 2008, Liu et al., 2009, Cruz et al., 2009, Glavic et al., 2009, Wang et al., 2010, Johansson et al., 2005, Nam et al., 2012, Wheeler et al., 2004, Karkali and Panayotou, 2012, Mathew et al., 2011) Velcro (Skaer, 1995.12.11) velcro
Secondary FlyBase IDs
	FBgn0004210 FBgn0011369 FBgn0011600
References ( 391 )
Generate a list of	All references relating to this gene ( 391 )
List References by type	Research paper  ( 270 ) Supplementary material  ( 13 ) Review  ( 33 ) Erratum  ( 1 ) Personal communication to FlyBase  ( 18 ) Abstract  ( 44 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 1 ) Curated genome annotation  ( 3 ) Stock list  ( 1 ) Website  ( 1 ) Letter  ( 1 ) Conference report  ( 1 ) Book  ( 1 )
Recent research papers ( 45 )
	Castillo et al., 2013, J. Insect Physiol. 59(2): 179--185 Immune gene transcription in Drosophila adult flies infected by entomopathogenic nematodes and their mutualistic bacteria. [FBrf0220790] Katsuyama and Paro, 2013, Mech. Dev. 130(2-3): 112--121 Innate immune cells are dispensable for regenerative growth of imaginal discs. [FBrf0220781] Rudrapatna et al., 2013, EMBO Rep. 14(2): 172--177 Caspase signalling in the absence of apoptosis drives Jnk-dependent invasion. [FBrf0220676] Bangi et al., 2012, EMBO Rep. 13(6): 569--576 Immune response to bacteria induces dissemination of Ras-activated Drosophila hindgut cells. [FBrf0218473] Chakrabarti et al., 2012, Cell Host Microbe 12(1): 60--70 Infection-induced host translational blockage inhibits immune responses and epithelial renewal in the Drosophila gut. [FBrf0218984] Chatterjee and Bohmann, 2012, PLoS ONE 7(4): e34063 A Versatile ΦC31 Based Reporter System for Measuring AP-1 and Nrf2 Signaling in Drosophila and in Tissue Culture. [FBrf0218067] Colombani et al., 2012, Science 336(6081): 582--585 Secreted peptide Dilp8 coordinates Drosophila tissue growth with developmental timing. [FBrf0218236] Dekanty et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109(50): 20549--20554 Aneuploidy-induced delaminating cells drive tumorigenesis in Drosophila epithelia. [FBrf0220285] Förster and Luschnig, 2012, Nat. Cell Biol. 14(5): 526--534 Src42A-dependent polarized cell shape changes mediate epithelial tube elongation in Drosophila. [FBrf0218206] Gonda et al., 2012, PLoS ONE 7(7): e42369 Drosophila Heat Shock Response Requires the JNK Pathway and Phosphorylation of Mixed Lineage Kinase at a Conserved Serine-Proline Motif. [FBrf0219015] Gowda et al., 2012, J. Biol. Chem. 287(21): 17554--17567 p53 Binding Prevents Phosphatase-mediated Inactivation of Diphosphorylated c-Jun N-terminal Kinase. [FBrf0218361] Jemc et al., 2012, Dev. Biol. 367(2): 114--125 raw Functions through JNK signaling and cadherin-based adhesion to regulate Drosophila gonad morphogenesis. [FBrf0218557] Karkali and Panayotou, 2012, Biochem. Biophys. Res. Commun. 418(2): 301--306 The Drosophila DUSP Puckered is phosphorylated by JNK and p38 in response to arsenite-induced oxidative stress. [FBrf0217429] Kelsey et al., 2012, J. Cell Sci. 125(6): 1393--1400 Schnurri regulates hemocyte function to promote tissue recovery after DNA damage. [FBrf0218143] Lim et al., 2012, PLoS ONE 7(5): e37028 Ebi/AP-1 Suppresses Pro-Apoptotic Genes Expression and Permits Long-Term Survival of Drosophila Sensory Neurons. [FBrf0218464] Lu et al., 2012, PLoS Biol. 10(7): e1001357 Niche-associated activation of rac promotes the asymmetric division of Drosophila female germline stem cells. [FBrf0218889] Marchal et al., 2012, PLoS ONE 7(3): e34310 The HIV-1 Vpu Protein Induces Apoptosis in Drosophila via Activation of JNK Signaling. [FBrf0218002] Meinander et al., 2012, EMBO J. 31(12): 2770--2783 Ubiquitylation of the initiator caspase DREDD is required for innate immune signalling. [FBrf0218688] Nam et al., 2012, EMBO J. 31(5): 1253--1265 Genetic evidence of a redox-dependent systemic wound response via Hayan Protease-Phenoloxidase system in Drosophila. [FBrf0217593] Pezzulo et al., 2012, PLoS ONE 7(8): e43777 Expression of Human Paraoxonase 1 Decreases Superoxide Levels and Alters Bacterial Colonization in the Gut of Drosophila melanogaster. [FBrf0219363] Poernbacher et al., 2012, Curr. Biol. 22(5): 389--396 Drosophila Pez acts in hippo signaling to restrict intestinal stem cell proliferation. [FBrf0217766] Rynes et al., 2012, Mol. Cell. Biol. 32(19): 3949--3962 Activating transcription factor 3 regulates immune and metabolic homeostasis. [FBrf0219385] Wandler and Guillemin, 2012, PLoS Pathog. 8(10): e1002939 Transgenic Expression of the Helicobacter pylori Virulence Factor CagA Promotes Apoptosis or Tumorigenesis through JNK Activation in Drosophila. [FBrf0219744] Wang et al., 2012, Sci. Rep. 2: 563 Akt signaling-associated metabolic effects of dietary gold nanoparticles in Drosophila. [FBrf0219113] Abruzzi et al., 2011, Genes Dev. 25(22): 2374--2386 Drosophila CLOCK target gene characterization: implications for circadian tissue-specific gene expression. [FBrf0216798] Belacortu et al., 2011, Gene Expr. Patterns 11(3-4): 190--201 Expression of Drosophila Cabut during early embryogenesis, dorsal closure and nervous system development. [FBrf0213309] Booker et al., 2011, BMC Genomics 12: 50 False negative rates in Drosophila cell-based RNAi screens: a case study. [FBrf0212969] Boyd et al., 2011, Free Radical Biol. Med. 50(11): 1669--1678 Nectarine promotes longevity in Drosophila melanogaster. [FBrf0213612] Friedman et al., 2011, Sci. Signal. 4(196): rs10 Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. [FBrf0216513] Glittenberg et al., 2011, Dis. Model Mech. 4(4): 515--525 Pathogen and host factors are needed to provoke a systemic host response to gastrointestinal infection of Drosophila larvae by Candida albicans. [FBrf0214008] Greenberg and Hatini, 2011, Mech. Dev. 128(1-2): 5--17 Systematic expression and loss-of-function analysis defines spatially restricted requirements for Drosophila RhoGEFs and RhoGAPs in leg morphogenesis. [FBrf0212976] Horn et al., 2011, Nat. Methods 8(4): 341--346 Mapping of signaling networks through synthetic genetic interaction analysis by RNAi. [FBrf0213352] Jezowska et al., 2011, Dev. Biol. 360(1): 143--159 A dual function of Drosophila capping protein on DE-cadherin maintains epithelial integrity and prevents JNK-mediated apoptosis. [FBrf0216680] Jiang et al., 2011, Cell Stem Cell 8(1): 84--95 EGFR/Ras/MAPK Signaling Mediates Adult Midgut Epithelial Homeostasis and Regeneration in Drosophila. [FBrf0212694] Jiang et al., 2011, Oncogene 30(29): 3248--3260 Sds22/PP1 links epithelial integrity and tumor suppression via regulation of myosin II and JNK signaling. [FBrf0214488] Marinho et al., 2011, Development 138(2): 349--357 The Drosophila Nol12 homologue viriato is a dMyc target that regulates nucleolar architecture and is required for dMyc-stimulated cell growth. [FBrf0212565] Mathew et al., 2011, Mol. Cell. Biol. 31(24): 4978--4993 Role for traf4 in polarizing adherens junctions as a prerequisite for efficient cell shape changes. [FBrf0216703] Ohsawa et al., 2011, Dev. Cell 20(3): 315--328 Elimination of Oncogenic Neighbors by JNK-Mediated Engulfment in Drosophila. [FBrf0213219] Opota et al., 2011, PLoS Pathog. 7(9): e1002259 Monalysin, a Novel ß-Pore-Forming Toxin from the Drosophila Pathogen Pseudomonas entomophila, Contributes to Host Intestinal Damage and Lethality. [FBrf0216341] Resnik-Docampo and de Celis, 2011, PLoS ONE 6(1): e14528 MAP4K3 Is a Component of the TORC1 Signalling Complex that Modulates Cell Growth and Viability in Drosophila melanogaster. [FBrf0212911] Rumpf et al., 2011, Development 138(6): 1153--1160 Neuronal remodeling and apoptosis require VCP-dependent degradation of the apoptosis inhibitor DIAP1. [FBrf0213078] Seisenbacher et al., 2011, PLoS Genet. 7(8): e1002168 MK2-Dependent p38b Signalling Protects Drosophila Hindgut Enterocytes against JNK-Induced Apoptosis under Chronic Stress. [FBrf0214607] Sinenko et al., 2011, EMBO Rep. 13(1): 83--89 Oxidative stress in the haematopoietic niche regulates the cellular immune response in Drosophila. [FBrf0217071] Suissa et al., 2011, J. Biol. Chem. 286(17): 15556--15564 The NAB-Brk Signal Bifurcates at JNK to Independently Induce Apoptosis and Compensatory Proliferation. [FBrf0213516] Tripura et al., 2011, Int. J. Dev. Biol. 55(6): 583--590 Regulation and activity of JNK signaling in the wing disc peripodial membrane during adult morphogenesis in Drosophila. [FBrf0216296] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...