FlyBase Gene Report: Dmel\Vrp1

General Information

Symbol

Dmel\Vrp1

Species

D. melanogaster

Name

Verprolin 1

Annotation Symbol

CG13503

Feature Type

protein_coding_gene

FlyBase ID

FBgn0243516

Gene Model Status

Stock Availability

11 publicly available

Gene Summary

Verprolin 1 (Vrp1) encodes the sole Drosophila homolog of the conserved Verprolin/WASp-interacting-protein family of actin regulators. It facilitates myoblast fusion in somatic muscles by recruiting the branched-actin polymerization machinery to sites of myoblast-myotube attachment and fusion. [Date last reviewed: 2019-03-21] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Interactive Fly

Also Known As

sltr, D-WIP, solitary, wip, Verprolin

Key Links

Genomic Location

Cytogenetic map

Sequence location

2R:22,106,107..22,122,562 [-]

Recombination map

2-98

RefSeq locus

NT_033778 REGION:22106107..22122562

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (9 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables actin filament binding

inferred from direct assay

(Kim et al., 2007)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (6 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

involved_in behavioral response to ethanol

inferred from mutant phenotype

(Kong et al., 2010)

involved_in myoblast fusion

inferred from mutant phenotype

(Berger et al., 2008, Kim et al., 2007, Massarwa et al., 2007, Estrada et al., 2006)

involved_in positive regulation of actin filament polymerization

inferred from direct assay

(Kim et al., 2007)

involved_in regulation of actin filament polymerization

inferred from mutant phenotype

(Rotkopf et al., 2011)

involved_in spermatid development

inferred from mutant phenotype

(Rotkopf et al., 2011)

involved_in visceral muscle development

inferred from mutant phenotype

(Eriksson et al., 2010)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Kim et al., 2007)

located_in filopodium tip

inferred from direct assay

(Berger et al., 2008)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

Protein Family (UniProt)

-

Protein Signatures (InterPro)

WH2 domain

Summaries

Gene Snapshot

Verprolin 1 (Vrp1) encodes the sole Drosophila homolog of the conserved Verprolin/WASp-interacting-protein family of actin regulators. It facilitates myoblast fusion in somatic muscles by recruiting the branched-actin polymerization machinery to sites of myoblast-myotube attachment and fusion. [Date last reviewed: 2019-03-21]

Summary (Interactive Fly)

facilitator of myoblast fusion - homolog of the conserved Verprolin/WASp Interacting Protein family of WASp-binding proteins - recruits the actin-polymerization machinery to sites of myoblast attachment and fusion

Interactive Fly

Gene Model and Products

Number of Transcripts

12

Number of Unique Polypeptides

7

Please see the JBrowse view of Dmel\Vrp1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.51

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.56

Sequence Ontology: Class of Gene

protein_coding_gene

(Chen, 2006)

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0071793

3823

751

FBtr0071791

3686

751

FBtr0071795

3345

751

FBtr0071794

3331

751

FBtr0071796

3263

761

FBtr0071792

3407

708

FBtr0113104

4017

852

FBtr0302295

3134

718

FBtr0302296

3410

809

FBtr0302297

3888

809

FBtr0302298

3216

708

FBtr0342980

3281

767

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

FBpp0071706

76.4

751

11.89

FBpp0071704

76.4

751

11.89

FBpp0071708

76.4

751

11.89

FBpp0071707

76.4

751

11.89

FBpp0071709

77.6

761

11.99

FBpp0071705

71.2

708

11.98

FBpp0112017

86.3

852

11.96

FBpp0291501

72.5

718

12.08

FBpp0291502

81.1

809

12.05

FBpp0291503

81.1

809

12.05

FBpp0291504

71.2

708

11.98

FBpp0309744

78.3

767

11.94

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

751 aa isoforms: Vrp1-PA, Vrp1-PB, Vrp1-PC, Vrp1-PD

708 aa isoforms: Vrp1-PF, Vrp1-PK

809 aa isoforms: Vrp1-PI, Vrp1-PJ

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

WH2 domain

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Vrp1 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

(Fisher et al., 2012)

embryonic stage 9 -- 10

(Fisher et al., 2012)

embryonic stage 11

embryonic precursor cell of adult muscle

(Massarwa et al., 2007)

embryonic stage 11 -- 12

dorsal pharyngeal muscle primordium

(Fisher et al., 2012)

somatic muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)

visceral muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)

(Fisher et al., 2012)

visceral mesoderm

(Eriksson et al., 2010)

embryonic stage 11 -- 14

embryonic somatic muscle

• cytoplasm

Comment: expressed in fusion-competent myoblasts at sites of fusion

(Kim et al., 2007)

embryonic visceral muscle

• cytoplasm

(Kim et al., 2007)

embryonic stage 12

fusion competent cell

(Massarwa et al., 2007)

embryonic stage 12 -- 14

somatic mesoderm

(Eriksson et al., 2010)

embryonic stage 12 -- 15

embryonic myoblast

(Massarwa et al., 2007)

embryonic stage 13 -- 16

dorsal pharyngeal muscle

(Fisher et al., 2012)

embryonic hindgut

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

embryonic/larval muscle system

(Fisher et al., 2012)

embryonic/larval somatic muscle

(Fisher et al., 2012)

Additional Descriptive Data

Vrp1 is expressed in somatic and visceral muscle starting in embryonic stage 11 and persists in somatic muscle until the end of stage 14 when fusion is completed. It is observed in fusion-competent cells but not in founder cells. It is found within the cytoplasm, specifically at sites of fusion.

(Kim et al., 2007)

Vrp1 expressionis absent during early stages, and is first observed at stage 11 in mesodermal muscle precursors, resolving into myoblast-specific expression that peaks at stage 14, then diminishes and disappears by stage 16.

(Massarwa et al., 2007)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

muscle attachment site

(Eriksson et al., 2010)

embryonic/larval somatic muscle

(Eriksson et al., 2010)

embryonic stage 11 -- 14

embryonic somatic muscle

• cytoplasm

Comment: expressed in fusion-competent myoblasts at sites of fusion

(Kim et al., 2007)

embryonic visceral muscle

• cytoplasm

(Kim et al., 2007)

fusion competent cell

(Eriksson et al., 2010)

visceral mesoderm

(Eriksson et al., 2010)

embryonic stage 14

fusion competent cell

(Jin et al., 2011)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Vrp1 protein expression is specific to fusion competent cells, and is not expressed in muscle founder cells.

(Jin et al., 2011)

Vrp1 is strongly expressed in muscles. It is found in both embryonic visceral mesoderm and somatic muscle as well as muscle attachment sites. Expression in the visceral mesoderm is specific to fusion competent cells and it is not found in muscle founder cells.

(Eriksson et al., 2010)

Vrp1 is expressed in somatic and visceral muscle starting in embryonic stage 11 and persists in somatic muscle until the end of stage 14 when fusion is completed. It is observed in fusion-competent cells but not in founder cells. It is found within the cytoplasm, specifically at sites of fusion.

(Kim et al., 2007)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Kim et al., 2007)

located_in filopodium tip

inferred from direct assay

(Berger et al., 2008)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Vrp1 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Vrp1

View exonic expression by tissue for Dmel\Vrp1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0243516

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0243516

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG13503-RB

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG13503

Flygut - An atlas of the Drosophila adult midgut

FBgn0243516

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Vrp1

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 11-12
Stages(s) 13-16

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 10 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 22 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Vrp1

Transgenic constructs containing regulatory region of Vrp1

Aberrations (Deficiencies and Duplications) ( 5 )

Inferred from experimentation ( 5 )

Gene disrupted in

(Eriksson et al., 2010)

(Ryder, 2004.4.26)

(Kim et al., 2007, Ryder, 2004.4.26)

(Massarwa et al., 2007)

(Kim et al., 2007)

Gene partially disrupted in

(Massarwa et al., 2007)

Inferred from location ( 0 )

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

Vrp1^f06715

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Vrp1^GD8042

viable, with Scer\GAL4^pnr-MD237

Vrp1^GD8042

Sterility

Allele

Vrp1^KO

male sterile, with Scer\GAL4^A3.eya

Vrp1^KK111261

male sterile, with Scer\GAL4^Mef2.PR, Vrp1^S1946/Vrp1^D30

Vrp1^UAS.cMa

male sterile (with Vrp1^D30), with Scer\GAL4^Mef2.PR, Vrp1^UAS.cMa

Vrp1^S1946

male sterile (with Vrp1^S1946), with Scer\GAL4^Mef2.PR, Vrp1^UAS.cMa

Vrp1^D30

Other Phenotypes

Allele

abnormal body color, with Scer\GAL4^pnr-MD237

Vrp1^GD8042

chemical sensitive

Vrp1^EY02177

hypoactive | conditional

Vrp1^EY02177

visible, with Scer\GAL4^pnr-MD237

Vrp1^GD8042

Phenotype manifest in

Allele

actin cytoskeleton

Vrp1^S1946

actin filament, with Scer\GAL4^Mef2.PR, Vrp1^S1946/Vrp1^D30

Vrp1^UAS.cMa

actin filament (with Vrp1^D30)

Vrp1^KO

actin filament (with Vrp1^D30), with Scer\GAL4^Mef2.PR, Vrp1^UAS.cMa

Vrp1^S1946

actin filament (with Vrp1^KO)

Vrp1^D30

actin filament (with Vrp1^S1946), with Scer\GAL4^Mef2.PR, Vrp1^UAS.cMa

Vrp1^D30

actin filament & fusion competent cell

Vrp1^S1946

actin filament & muscle founder cell

Vrp1^S1946

dorsal acute muscle | embryonic stage

Vrp1^D30

dorsal pharyngeal muscle

Vrp1^D30

embryonic/larval somatic muscle, with Scer\GAL4^twi.PB

Vrp1^ΔC.UAS

embryonic myoblast

embryonic somatic muscle

Vrp1^f06715

exocytic vesicle & fusion competent cell

Vrp1^S1946

fusion competent cell

head cyst cell, with Scer\GAL4^Mef2.PR, Vrp1^S1946/Vrp1^D30

Vrp1^UAS.cMa

head cyst cell (with Vrp1^D30)

Vrp1^KO

head cyst cell (with Vrp1^D30), with Scer\GAL4^Mef2.PR, Vrp1^UAS.cMa

Vrp1^S1946

head cyst cell (with Vrp1^KO)

Vrp1^D30

head cyst cell (with Vrp1^S1946), with Scer\GAL4^Mef2.PR, Vrp1^UAS.cMa

Vrp1^D30

mesothoracic tergum, with Scer\GAL4^pnr-MD237

Vrp1^GD8042

muscle founder cell

myoblast, with Scer\GAL4^twi.PB

Vrp1^ΔC.UAS

myoblast | embryonic stage

plasma membrane | embryonic stage

seminal vesicle

Vrp1^KO

seminal vesicle, with Scer\GAL4^A3.eya

Vrp1^KK111261

seminal vesicle, with Scer\GAL4^Mef2.PR, Vrp1^S1946/Vrp1^D30

Vrp1^UAS.cMa

seminal vesicle (with Vrp1^D30), with Scer\GAL4^Mef2.PR, Vrp1^UAS.cMa

Vrp1^S1946

seminal vesicle (with Vrp1^S1946), with Scer\GAL4^Mef2.PR, Vrp1^UAS.cMa

Vrp1^D30

Vrp1^KO

spermatid cyst, with Scer\GAL4^A3.eya

Vrp1^KK111261

spermatid cyst, with Scer\GAL4^Mef2.PR, Vrp1^S1946/Vrp1^D30

Vrp1^UAS.cMa

spermatid cyst (with Vrp1^D30)

Vrp1^KO

spermatid cyst (with Vrp1^D30), with Scer\GAL4^Mef2.PR, Vrp1^UAS.cMa

Vrp1^S1946

spermatid cyst (with Vrp1^KO)

Vrp1^D30

spermatid cyst (with Vrp1^S1946), with Scer\GAL4^Mef2.PR, Vrp1^UAS.cMa

Vrp1^D30

visceral muscle fiber | embryonic stage

Vrp1^D30

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (4)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

5 of 15

Yes

Yes

4 of 15

No

Yes

2 of 15

No

Yes

1 of 15

No

No

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (3)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

5 of 15

Yes

Yes

4 of 15

No

Yes

3 of 15

No

Yes

Rattus norvegicus (Norway rat) (3)

4 of 13

Yes

Yes

1 of 13

No

Yes

1 of 13

No

Yes

Xenopus tropicalis (Western clawed frog) (2)

4 of 12

Yes

Yes

4 of 12

Yes

Yes

Danio rerio (Zebrafish) (5)

4 of 15

Yes

Yes

4 of 15

Yes

Yes

4 of 15

Yes

Yes

3 of 15

No

Yes

3 of 15

No

Yes

Caenorhabditis elegans (Nematode, roundworm) (5)

4 of 15

Yes

Yes

1 of 15

No

No

1 of 15

No

No

1 of 15

No

Yes

1 of 15

No

No

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (1)

6 of 15

Yes

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

3 of 12

Yes

Yes

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091909AK )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Dsuz\DS10_00003519

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Drosophila persimilis

Drosophila willistoni

Drosophila virilis

Drosophila mojavensis

Drosophila grimshawi

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150ECM )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Glossina morsitans

Tsetse fly

Gmor\GMOY003107

Glossina morsitans

Tsetse fly

Gmor\GMOY006562

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_09891

Mayetiola destructor

Hessian fly

Mdes\Mdes005010

Mayetiola destructor

Hessian fly

Mdes\Mdes015190

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL008154

Anopheles darlingi

American malaria mosquito

Adar\ADAC009994

Anopheles gambiae

Malaria mosquito

Agam\AGAP001886

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ008382

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( None identified )

No non-Dipteran orthologies identified

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0HPY )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Ixodes scapularis

Black-legged tick

Isca\ISCW023190

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )

No non-Arthropod Metazoa orthologies identified

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (1)

Gene Symbol

Score

Source

Alignment

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

WIPF1; WAS/WASL interacting protein family member 1

5 of 15

WISKOTT-ALDRICH SYNDROME 2; WAS2

WIPF2; WAS/WASL interacting protein family member 2

4 of 15

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

anti tag coimmunoprecipitation, anti tag western blot, pull down

(Kim et al., 2007)

anti tag coimmunoprecipitation, anti tag western blot, western blot, experimental knowledge based, pull down

(Kaipa et al., 2013, Guruharsha et al., 2011, Jin et al., 2011, Kim et al., 2007)

anti tag coimmunoprecipitation, western blot, anti tag western blot

(Jin et al., 2011)

two hybrid, anti tag coimmunoprecipitation, western blot

(Kaipa et al., 2013)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

(Kaipa et al., 2013)

enhanceable

(Sens et al., 2010, Berger et al., 2008)

suppressible

(Massarwa et al., 2007)

enhanceable

(Kaipa et al., 2013)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

(Sens et al., 2010)

enhanceable

(Kaipa et al., 2013)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

63141

DroID - A comprehensive database of gene and protein interactions.

FBgn0243516

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

37521

MIST (genetic) - An integrated Molecular Interaction Database

Vrp1

MIST (protein-protein) - An integrated Molecular Interaction Database

Vrp1

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Endocytosis

Genomic Location and Detailed Mapping Data

Chromosome (arm)

2R

Recombination map

2-98

Cytogenetic map

Sequence location

2R:22,106,107..22,122,562 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

58B8-58B9

Limits computationally determined from genome sequence between P{EP}CG6393^EP2457 and P{PZ}ari-2⁰⁷⁷⁶⁸

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-98

(FlyBase, 2016)

2-103.7

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (11)

26101

w¹¹¹⁸; Mi{ET1}Vrp1^MB08225

43746

y¹ w^*; Mi{MIC}Vrp1^MI07644

56117

y¹ w^*; Mi{MIC}Vrp1^MI11078

15403

y¹ w^67c23; P{EPgy2}Vrp1^EY02177

78948

y¹ w^*; TI{CRIMIC.TG4.0}Vrp1^{CR00552-TG4.0}

36704

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01593}attP2

38201

y¹ sc^* v¹ sev²¹; P{TRiP.GL00640}attP40

23236

y¹ w^*; P{UAST-YFP.Rab1.S25N}mei-S332⁰⁴ Vrp1⁰⁴

v102253

P{KK111261}VIE-260B

v23888

w¹¹¹⁸; P{GD8042}v23888

v318860

PBac{fTRG01357.sfGFP-TVPTBF}VK00033

Genomic Clones (16)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (125)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0243516

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0243516

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

37521

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Berger et al., 2008, Massarwa et al., 2007)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: D-WIP CG13503

(Schejter, 2007.2.26)

Source for identity of: sltr CG13503

(Chen, 2007.2.26)

Source for identity of: Vrp1 CG13503

(Paunola et al., 2002)

Source for database merge of

Additional comments

Other Comments

EM analysis reveals abnormal accumulations of prefusion vesicles along the apposing membranes of adhering myoblasts in CG13503 mutant embryos, indicating a role of the actin cytoskeleton in targeting and fusion of these vesicles to proper sites on the plasma membrane.

(Chen, 2007)

Vrp1 is required for F-actin accumulation at sites of fusion in fusion-competent cells during muscle formation in the embryo.

(Kim et al., 2007)

CG13503, which is expressed specifically in myoblasts, associates with both the CG13503-Arp2/3 system, and with the myoblast fusion receptors kirre and sns, thereby recruiting the actin polymerization machinery to sites of myoblast attachment and fusion.

(Schejter et al., 2007)

Embryonic injection of dsRNA made from templates generated with primers directed against this gene results in a reduction in myoblast function, with an overall reduction and disorganisation of muscle fibers.

(Estrada et al., 2006)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R⁺ cells: reduced F-actin and altered cell shape.

(Kiger et al., 2003)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 72 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

37521

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0243516

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0243516

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG13503

Flygut - An atlas of the Drosophila adult midgut

FBgn0243516

FlyMine - An integrated database for Drosophila genomics

FBgn0243516

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

37521

InterPro - A database of protein families, domains and functional sites

WH2 domain

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG13503

modMine - A data warehouse for the modENCODE project

FBgn0243516

Linkouts

BioGRID - A database of protein and genetic interactions.

63141

DroID - A comprehensive database of gene and protein interactions.

FBgn0243516

DRSC - Results frm RNAi screens

FBgn0243516

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0243516

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG13503-RB

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0243516

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

37521

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Endocytosis

MIST (genetic) - An integrated Molecular Interaction Database

Vrp1

MIST (protein-protein) - An integrated Molecular Interaction Database

Vrp1

Synonyms and Secondary IDs (30)

Reported As

Symbol Synonym

CG13503

(McEachern et al., 2014, Abmayr and Pavlath, 2012, Ni et al., 2011.7.19, Eriksson et al., 2010, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Lebo et al., 2009, Chen, 2007.2.26, Kim et al., 2007, Massarwa et al., 2007, Schejter, 2007.2.26, Zhang et al., 2007, Estrada et al., 2006, Johansson et al., 2005)

D-WIP

(Mukherjee et al., 2011, Rotkopf et al., 2011, Bulchand et al., 2010, Gildor et al., 2009, Massarwa et al., 2007, Massarwa et al., 2007, Richardson et al., 2007, Schejter, 2007.2.26, Schejter et al., 2007)

DWIP

(Martin, 2016)

DWip

(Bothe and Baylies, 2016)

Dm_Vrp1

(Paunola et al., 2002)

Sltr

(Lee and Chen, 2019, Rodal et al., 2015, Abmayr and Pavlath, 2012)

Vrp1

(Teumer et al., 2019, Gene Disruption Project members, 2018-, Hamp et al., 2016, Sarov et al., 2016, Ashwal-Fluss et al., 2014, Popichenko et al., 2013, Abmayr and Pavlath, 2012, Japanese National Institute of Genetics, 2012.5.21, Friedman et al., 2011, Eriksson et al., 2010, Berger et al., 2008, Paunola et al., 2002)

WIP

(Lee and Chen, 2019, Dubey et al., 2016, Reed et al., 2012, Zoller and Schulz, 2012, Jin et al., 2011, Wolfstetter et al., 2009)

Wip

(Abmayr and Pavlath, 2012)

dWIP

(Rodal et al., 2015, Sens et al., 2010, Sens and Chen, 2008)

dwip

(Kim et al., 2021)

sltr

(Zhang et al., 2020, Deng et al., 2015, Kim et al., 2015, Duan et al., 2012, Jin et al., 2011, Bulchand et al., 2010, Sens et al., 2010, Berger et al., 2008, Chen, 2007, Chen, 2007.2.26, Kim et al., 2007, Chen, 2006)

solas

(Estrada et al., 2006)

vrp

(Álvarez-Fernández et al., 2015)

vrp1

(Dubois et al., 2016, Hamp et al., 2016, Kaipa et al., 2013, Bulchand et al., 2010, Berger et al., 2008)

wip

(Hamp et al., 2016, Haralalka et al., 2014, Rudolf et al., 2014, Kaipa et al., 2013, Hornbruch-Freitag et al., 2011, Berger et al., 2008)

Name Synonyms

CG13503

D-WASp-interacting protein

(Gildor et al., 2009)

Drosophila WIP

(Chen, 2007)

Solitary

(Bulchand et al., 2010)

Verprolin

(Reed et al., 2012, Eriksson et al., 2010, Kong et al., 2010)

Verprolin 1

(McEachern et al., 2014, Bulchand et al., 2010, Lebo et al., 2009)

Verprolin1

(Wolfstetter et al., 2009)

WASP-interacting protein

(Hornbruch-Freitag et al., 2011)

WASp Interacting Protein

(Schejter, 2007.2.26, Schejter et al., 2007)

WASp interacting protein

(Bulchand et al., 2010)

WASp-interacting protein

(Dubey et al., 2016)

solitary

(Jin et al., 2011, Sens and Chen, 2008, Chen, 2007, Chen, 2007.2.26, Kim et al., 2007, Chen, 2006)

veroprolin

(Reed et al., 2012)

verprolin

(Estrada et al., 2006, Paunola et al., 2002)

verprolin 1

(Schäfer et al., 2008)

Secondary FlyBase IDs

FBgn0034695
FBgn0086255

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (84)