sltr, D-WIP, solitary, wip, dWIP
facilitator of myoblast fusion - homolog of the conserved Verprolin/WASp Interacting Protein family of WASp-binding proteins - recruits the actin-polymerization machinery to sites of myoblast attachment and fusion
Please see the GBrowse view of Dmel\Vrp1 or the JBrowse view of Dmel\Vrp1 for information on other features
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Gene model reviewed during 5.51
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.56
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Vrp1 using the Feature Mapper tool.
Comment: expressed in fusion-competent myoblasts at sites of fusion
Comment: maternally deposited
Comment: reported as muscle system primordium
Comment: reported as muscle system primordium
Vrp1 is expressed in somatic and visceral muscle starting in embryonic stage 11 and persists in somatic muscle until the end of stage 14 when fusion is completed. It is observed in fusion-competent cells but not in founder cells. It is found within the cytoplasm, specifically at sites of fusion.
Vrp1 expressionis absent during early stages, and is first observed at stage 11 in mesodermal muscle precursors, resolving into myoblast-specific expression that peaks at stage 14, then diminishes and disappears by stage 16.
Comment: expressed in fusion-competent myoblasts at sites of fusion
Vrp1 protein expression is specific to fusion competent cells, and is not expressed in muscle founder cells.
Vrp1 is strongly expressed in muscles. It is found in both embryonic visceral mesoderm and somatic muscle as well as muscle attachment sites. Expression in the visceral mesoderm is specific to fusion competent cells and it is not found in muscle founder cells.
Vrp1 is expressed in somatic and visceral muscle starting in embryonic stage 11 and persists in somatic muscle until the end of stage 14 when fusion is completed. It is observed in fusion-competent cells but not in founder cells. It is found within the cytoplasm, specifically at sites of fusion.
GBrowse - Visual display of RNA-Seq signals
View Dmel\Vrp1 in GBrowse 2
2-98
2-98
2-103.7
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
polyclonal
Source for identity of: D-WIP CG13503
Source for identity of: sltr CG13503
Source for identity of: Vrp1 CG13503
EM analysis reveals abnormal accumulations of prefusion vesicles along the apposing membranes of adhering myoblasts in CG13503 mutant embryos, indicating a role of the actin cytoskeleton in targeting and fusion of these vesicles to proper sites on the plasma membrane.
Vrp1 is required for F-actin accumulation at sites of fusion in fusion-competent cells during muscle formation in the embryo.
Embryonic injection of dsRNA made from templates generated with primers directed against this gene results in a reduction in myoblast function, with an overall reduction and disorganisation of muscle fibers.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R+ cells: reduced F-actin and altered cell shape.
