• Home
• Tools
  • Tools Overview
  • Query Tools

    
    • QuickSearch
    • QueryBuilder
    • TermLink
    • CytoSearch
    • RNA-Seq Search
    • Interactions Browser
    • ImageBrowse
    • Find A Person
    • Google™ FlyBase
  • Genomic/Map Tools

    
    • BLAST
    • GBrowse
    • GBrowse 2 Beta
    • CytoSearch
    • Feature Mapper
    • Aberrations Maps
    • Chromosome Maps
    • Coordinates Converter
  • Retrieve/Convert

    
    • Batch Download
    • Coordinates Converter
    • ID Converter
  • Data Submission

    
    • Fast-Track Your Paper
    • Submit Personal Comm
    • Add A New Person
    • Update Person Info
  • People

    
    • Find A Person
    • Add A New Person
    • Update Person Info
• Files
  • Files Overview
  • Current release
  • Archived releases
  • Map Conversion
  • FTP site:
  • Releases (FTP)
  • Genomes (FTP)
• Species
  • Phylogeny
  • Sequenced Species
  • Synteny Table
  • Color Illustrations
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All Resources
  • Model Organisms
  • Stock Collections
  • Vectors & Constructs
  • Other links:
  • BDGP
  • DGRC
  • DIS by issue
  • FlyExpress
  • Interactive Fly
  • modENCODE
  • Textpresso for Fly
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • FlyBase Guides
  • Video Tutorials
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Contact FlyBase
• Archives
  • Prior Release
  • Other Archives
  • Coordinate Converter

Gene Dmel\vari

General Information
Symbol	Dmel\vari	Species	D. melanogaster
Name	varicose	Annotation symbol	CG9326
Feature type	protein_coding_gene	FlyBase ID	FBgn0250785
Gene Model Status	Current	Stock availability	17 publicly available
Also Known As	l(2)03953, 38E.21, szar
Genomic Location
Chromosome (arm)	2L	Recombination map	2-
Cytogenetic map	39D3-40B1	Sequence location	2L:20,788,662..20,797,042 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene varicose is referred to in FlyBase by the symbol Dmel\vari (CG9326, FBgn0250785). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: guanylate kinase activity. There is experimental evidence that it is involved in the biological process: dorsal trunk growth, open tracheal system; regulation of tube size, open tracheal system; open tracheal system development; regulation of tube length, open tracheal system; septate junction assembly. 34 alleles are reported. The phenotypes of these alleles are annotated with: organ system; organ system subdivision; adult segment; late extended germ band embryo; non-connected developing system; external compound sense organ; apical junction complex; tracheal branch primordium; tracheal system; adult. It has 5 annotated transcripts and 5 annotated polypeptides. Protein features are: Guanylate kinase; Guanylate kinase, conserved site; Guanylate kinase/L-type calcium channel; L27; L27, C-terminal; PDZ domain; Src homology-3 domain; Variant SH3. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed within 06-18 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult crop. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, larval/adult hindgut, larval/adult salivary gland, larval trachea, adult male accessory gland, larval carcass. Comments on Affy2 ProbeSet: ProbeSet 1625358_s_at partially aligns to an exonic region common to each of the 3 FlyBase-annotated transcript isoforms of vari: 11 out of 13 probes fall into this exonic region. Gene sequence location is 2L:20788662..20797042.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 39D3-40B1   Left limit from non-inclusion within Df(2L)TW84 (FBrf0128394) Right limit from inclusion within Df(2L)TW161 (FBrf0128394)
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location	2- (Kozlova et al., 1998)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\vari for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.47 Low-frequency RNA-Seq exon junction(s) not annotated. Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) vari-RB FBtr0089947  NM_165342   2610   636 vari-RC FBtr0089946  NM_165343   2320   469 vari-RD FBtr0089945  NM_206011   2717   615 vari-RE FBtr0335146  NM_001273715   2535   611 vari-RF FBtr0335501  NM_001273716   2379   469
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein vari-PB   FBpp0088886   72.4   636   7.34     NP_724288   AAN11089 vari-PC   FBpp0088885   53.4   469   6.43     NP_724289   AAN11090 vari-PD   FBpp0088884   70.3   615   6.95     NP_995733   AAF53925 vari-PE   FBpp0307145   69.4   611   6.95     NP_001260644   AGB93179 vari-PF   FBpp0307469   53.4   469   6.43     NP_001260645   AGB93180
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • P-loop containing nucleoside triphosphate hydrolase (IPR027417) L27, C-terminal (IPR014775) Variant SH3 (IPR011511) Guanylate kinase/L-type calcium channel (IPR008145) Guanylate kinase (IPR008144) L27 (IPR004172) PDZ domain (IPR001478) Src homology-3 domain (IPR001452) Guanylate kinase, conserved site (IPR020590)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA440379   AE014134 AAF53925   AE014134 AAN11089   AE014134 AAN11090   AC004759   AE014134 AGB93179   AE014134 AGB93180   AI294764   AI295323   AY089616 AAL90354   AY121709 AAM52036   BI162547   BI162548   BI163108   BI167166   BI168427   BI169780   BI171067   BI171437   BI171727   BI213760   BI214375   BI214672   BI216442   BI216452   BI228067   BI228960   BI230744   BI230913   BI234746   BI235604   BI237742   BI238457   BI239155   BI239206   BI239519   BI242401   BI242409   BI243180   BI243796   BI356095   BI356096   BI356103   BI358331   BI362397   BI362877   BI363208   BI366344   BI366345   BI366586   BI368631   BI371394   BI373418   BI373424   BI374097   BI374240   BI374855   BI482940   BI484009   BI484017   BI484039   BI486864   BI608989   BI610069   BI613575   BI631557   BI631632   BI631801   BI631879   BI632978   BP544132   BP544133   BP551145   BP551825   BP551826   BT056231   BT125020   BT132965   CA804853   CO177571   CO188299   CO264018   CO265290   CO266415   CO267346   CO273110   CO273240   CO281492   CO295519   CO295846   CO298572   CO311239   CO316020   CO322334   CO341177   CO341318   CZ477484   CZ477604   CZ477771   CZ479724   DQ787101 ABH06960   DQ787102 ABH06961   DQ787103 ABH06962   DQ787104 ABH06963   DQ787105 ABH06964   DQ787106 ABH06965   DQ787107 ABH06966   DQ787108 ABH06967   EC054575   EC055812   EC056377   EC057292   EC058068   EC061956   EC064082   EC074427   EC199155   EC212806   EC236707   EC237203   EC238455   EC243809   EC243835   EC244657   EC244889   EC255952   FD586428   FD586610   FD586796   FD586835   FD587084   GH738896   GH745578   GH761980   GH767746   GH768567   GH769859   GH944210   GH950072   GH952903   GH958005   GH959569   GH961613   GH964503   GH966731   GH967431   GH976059   GH977364   GH981501   GH984407   L80120
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VIJ4 Q7KT16 Q2PDR9 A1XXK0 A1XXK7 A1XXK3 Q8SXI6 A1XXK4 A1XXK1 A1XXK2 A1XXK5 Q8MR96
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism   (Fisher et al., 2012) embryonic stage 11 -- 12   dorsal epidermis primordium   (Fisher et al., 2012) foregut primordium   (Fisher et al., 2012) hindgut proper primordium   (Fisher et al., 2012) tracheal primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   atrium   (Fisher et al., 2012) embryonic dorsal epidermis   (Fisher et al., 2012) embryonic epipharynx   (Fisher et al., 2012) embryonic esophagus   (Fisher et al., 2012) embryonic foregut   (Fisher et al., 2012) embryonic head epidermis   (Fisher et al., 2012) embryonic hindgut   (Fisher et al., 2012) embryonic hypopharynx   (Fisher et al., 2012) embryonic ventral epidermis   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
lateral plasma membrane inferred from direct assay (Moyer and Jacobs, 2008) septate junction inferred from direct assay (Wu et al., 2007, Bachmann et al., 2008)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult crop. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, larval/adult hindgut, larval/adult salivary gland, larval trachea, adult male accessory gland, larval carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    46.525 Larval Midgut    26.9 Larval Hindgut    150.7 Larval Malpighian Tubules    21.8 Larval Fat Body    20.8 Larval Salivary Gland    182 Larval Trachea    232.05 Larval Carcass    134.6 Adult Head    161.5 Adult Eye    198.4 Adult Brain    144.1 Adult Thoracic-Abdominal Ganglion    198.5 Adult Crop    599.1 Adult Midgut    29.8 Adult Hindgut    270.9 Adult Malpighian Tubules    7.3 Adult Fat Body    42.2 Adult Salivary Gland    153.8 Adult Heart    52.425 Adult VirginFemale Spermatheca    22.7 Adult InseminatedFemale Spermatheca    24.7 Adult Ovary    53.8 Adult Testis    22.9 Adult Male Accessory Gland    177.7 Adult Carcass    68.9 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    46.525 Larval Midgut    26.9 Larval Hindgut    150.7 Larval Malpighian Tubules    21.8 Larval Fat Body    20.8 Larval Salivary Gland    182 Larval Trachea    232.05 Larval Carcass    134.6 Adult Head    161.5 Adult Eye    198.4 Adult Brain    144.1 Adult Thoracic-Abdominal Ganglion    198.5 Adult Crop  (599.1) Adult Midgut    29.8 Adult Hindgut    270.9 Adult Malpighian Tubules    7.3 Adult Fat Body    42.2 Adult Salivary Gland    153.8 Adult Heart    52.425 Adult VirginFemale Spermatheca    22.7 Adult InseminatedFemale Spermatheca    24.7 Adult Ovary    53.8 Adult Testis    22.9 Adult Male Accessory Gland    177.7 Adult Carcass    68.9 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    46.525 Larval Midgut    26.9 Larval Hindgut    150.7 Larval Malpighian Tubules    21.8 Larval Fat Body    20.8 Larval Salivary Gland    182 Larval Trachea    232.05 Larval Carcass    134.6 Adult Head    161.5 Adult Eye    198.4 Adult Brain    144.1 Adult Thoracic-Abdominal Ganglion    198.5 Adult Crop    599.1 Adult Midgut    29.8 Adult Hindgut    270.9 Adult Malpighian Tubules    7.3 Adult Fat Body    42.2 Adult Salivary Gland    153.8 Adult Heart    52.425 Adult VirginFemale Spermatheca    22.7 Adult InseminatedFemale Spermatheca    24.7 Adult Ovary    53.8 Adult Testis    22.9 Adult Male Accessory Gland    177.7 Adult Carcass    68.9 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    46.525 Larval Midgut    26.9 Larval Hindgut    150.7 Larval Malpighian Tubules    21.8 Larval Fat Body    20.8 Larval Salivary Gland    182 Larval Trachea    232.05 Larval Carcass    134.6 Adult Head    161.5 Adult Eye    198.4 Adult Brain    144.1 Adult Thoracic-Abdominal Ganglion    198.5 Adult Crop    599.1 Adult Midgut    29.8 Adult Hindgut    270.9 Adult Malpighian Tubules    7.3 Adult Fat Body    42.2 Adult Salivary Gland    153.8 Adult Heart    52.425 Adult VirginFemale Spermatheca    22.7 Adult InseminatedFemale Spermatheca    24.7 Adult Ovary    53.8 Adult Testis    22.9 Adult Male Accessory Gland    177.7 Adult Carcass    68.9 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    46.525 Larval Midgut    26.9 Larval Hindgut    150.7 Larval Malpighian Tubules    21.8 Larval Fat Body    20.8 Larval Salivary Gland    182 Larval Trachea    232.05 Larval Carcass    134.6 Adult Head    161.5 Adult Eye    198.4 Adult Brain    144.1 Adult Thoracic-Abdominal Ganglion    198.5 Adult Crop    599.1 Adult Midgut    29.8 Adult Hindgut    270.9 Adult Malpighian Tubules    7.3 Adult Fat Body    42.2 Adult Salivary Gland    153.8 Adult Heart    52.425 Adult VirginFemale Spermatheca    22.7 Adult InseminatedFemale Spermatheca    24.7 Adult Ovary    53.8 Adult Testis    22.9 Adult Male Accessory Gland    177.7 Adult Carcass    68.9 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    46.525 Larval Midgut    26.9 Larval Hindgut    150.7 Larval Malpighian Tubules    21.8 Larval Fat Body    20.8 Larval Salivary Gland    182 Larval Trachea    232.05 Larval Carcass    134.6 Adult Head    161.5 Adult Eye    198.4 Adult Brain    144.1 Adult Thoracic-Abdominal Ganglion    198.5 Adult Crop  599.1 Adult Midgut    29.8 Adult Hindgut    270.9 Adult Malpighian Tubules    7.3 Adult Fat Body    42.2 Adult Salivary Gland    153.8 Adult Heart    52.425 Adult VirginFemale Spermatheca    22.7 Adult InseminatedFemale Spermatheca    24.7 Adult Ovary    53.8 Adult Testis    22.9 Adult Male Accessory Gland    177.7 Adult Carcass    68.9 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    46.525 Larval Midgut    26.9 Larval Hindgut    150.7 Larval Malpighian Tubules    21.8 Larval Fat Body    20.8 Larval Salivary Gland    182 Larval Trachea    232.05 Larval Carcass    134.6 Adult Head    161.5 Adult Eye    198.4 Adult Brain    144.1 Adult Thoracic-Abdominal Ganglion    198.5 Adult Crop    599.1 Adult Midgut    29.8 Adult Hindgut    270.9 Adult Malpighian Tubules    7.3 Adult Fat Body    42.2 Adult Salivary Gland    153.8 Adult Heart    52.425 Adult VirginFemale Spermatheca    22.7 Adult InseminatedFemale Spermatheca    24.7 Adult Ovary    53.8 Adult Testis    22.9 Adult Male Accessory Gland    177.7 Adult Carcass    68.9 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    46.525 Larval Midgut    26.9 Larval Hindgut    150.7 Larval Malpighian Tubules    21.8 Larval Fat Body    20.8 Larval Salivary Gland    182 Larval Trachea    232.05 Larval Carcass    134.6 Adult Head    161.5 Adult Eye    198.4 Adult Brain    144.1 Adult Thoracic-Abdominal Ganglion    198.5 Adult Crop    599.1 Adult Midgut    29.8 Adult Hindgut    270.9 Adult Malpighian Tubules    7.3 Adult Fat Body    42.2 Adult Salivary Gland    153.8 Adult Heart    52.425 Adult VirginFemale Spermatheca    22.7 Adult InseminatedFemale Spermatheca    24.7 Adult Ovary    53.8 Adult Testis    22.9 Adult Male Accessory Gland    177.7 Adult Carcass    68.9 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    161.5   NA  Eye    198.4   NA  Brain    144.1   46.525  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    198.5   NA  Crop    599.1   26.9  Midgut    29.8   150.7  Hindgut    270.9   no informative data  Malpighian Tubules    7.3   no informative data  Fat Body    42.2   182  Salivary Gland    153.8   NA  Heart    52.425   232.05  Trachea    NA   NA  VirginFemale Spermatheca    22.7   NA  InseminatedFemale Spermatheca    24.7   NA  Ovary    53.8   NA  Testis    22.9   NA  Male Accessory Gland    177.7   134.6  Carcass    68.9 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    47 central nervous system, larvae L3    17 central nervous system, pupae P8    28 head, virgin 1-day female    25 head, virgin 4-day female    17 head, virgin 20-day female    20 head, mated 1-day female    17 head, mated 4-day female    14 head, mated 20-day female    15 head, mated 1-day male    38 head, mated 4-day male    24 head, mated 20-day male    26 salivary gland, larvae L3 wandering    6 salivary gland, white prepupae    10 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    7 digestive system, 4-day adult    5 digestive system, 20-day adult    5 fat body, larvae L3 wandering    7 fat body, white prepupae    8 fat body, pupae P8    29 carcass, larvae L3 wandering    39 carcass, 1-day adult    10 carcass, 4-day adult    9 carcass, 20-day adult    10 ovary, virgin 4-day female    10 ovary, mated 4-day female    6 testis, mated 4-day male    7 accessory gland, mated 4-day male    16 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (47) central nervous system, larvae L3    17 central nervous system, pupae P8  (28) head, virgin 1-day female    25 head, virgin 4-day female    17 head, virgin 20-day female    20 head, mated 1-day female    17 head, mated 4-day female    14 head, mated 20-day female    15 head, mated 1-day male  (38) head, mated 4-day male    24 head, mated 20-day male  26 salivary gland, larvae L3 wandering    6 salivary gland, white prepupae    10 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    7 digestive system, 4-day adult    5 digestive system, 20-day adult    5 fat body, larvae L3 wandering    7 fat body, white prepupae    8 fat body, pupae P8  (29) carcass, larvae L3 wandering  (39) carcass, 1-day adult    10 carcass, 4-day adult    9 carcass, 20-day adult    10 ovary, virgin 4-day female    10 ovary, mated 4-day female    6 testis, mated 4-day male    7 accessory gland, mated 4-day male    16 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    47 central nervous system, larvae L3    17 central nervous system, pupae P8    28 head, virgin 1-day female    25 head, virgin 4-day female    17 head, virgin 20-day female    20 head, mated 1-day female    17 head, mated 4-day female    14 head, mated 20-day female    15 head, mated 1-day male    38 head, mated 4-day male    24 head, mated 20-day male    26 salivary gland, larvae L3 wandering    6 salivary gland, white prepupae    10 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    7 digestive system, 4-day adult    5 digestive system, 20-day adult    5 fat body, larvae L3 wandering    7 fat body, white prepupae    8 fat body, pupae P8    29 carcass, larvae L3 wandering    39 carcass, 1-day adult    10 carcass, 4-day adult    9 carcass, 20-day adult    10 ovary, virgin 4-day female    10 ovary, mated 4-day female    6 testis, mated 4-day male    7 accessory gland, mated 4-day male    16 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    47 central nervous system, larvae L3    17 central nervous system, pupae P8    28 head, virgin 1-day female    25 head, virgin 4-day female    17 head, virgin 20-day female    20 head, mated 1-day female    17 head, mated 4-day female    14 head, mated 20-day female    15 head, mated 1-day male    38 head, mated 4-day male    24 head, mated 20-day male    26 salivary gland, larvae L3 wandering    6 salivary gland, white prepupae    10 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    7 digestive system, 4-day adult    5 digestive system, 20-day adult    5 fat body, larvae L3 wandering    7 fat body, white prepupae    8 fat body, pupae P8    29 carcass, larvae L3 wandering    39 carcass, 1-day adult    10 carcass, 4-day adult    9 carcass, 20-day adult    10 ovary, virgin 4-day female    10 ovary, mated 4-day female    6 testis, mated 4-day male    7 accessory gland, mated 4-day male    16 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    47 central nervous system, larvae L3    17 central nervous system, pupae P8    28 head, virgin 1-day female    25 head, virgin 4-day female    17 head, virgin 20-day female    20 head, mated 1-day female    17 head, mated 4-day female    14 head, mated 20-day female    15 head, mated 1-day male    38 head, mated 4-day male    24 head, mated 20-day male    26 salivary gland, larvae L3 wandering    6 salivary gland, white prepupae    10 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    7 digestive system, 4-day adult    5 digestive system, 20-day adult    5 fat body, larvae L3 wandering    7 fat body, white prepupae    8 fat body, pupae P8    29 carcass, larvae L3 wandering    39 carcass, 1-day adult    10 carcass, 4-day adult    9 carcass, 20-day adult    10 ovary, virgin 4-day female    10 ovary, mated 4-day female    6 testis, mated 4-day male    7 accessory gland, mated 4-day male    16 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (47) central nervous system, larvae L3    17 central nervous system, pupae P8  28 head, virgin 1-day female    25 head, virgin 4-day female    17 head, virgin 20-day female    20 head, mated 1-day female    17 head, mated 4-day female    14 head, mated 20-day female    15 head, mated 1-day male  (38) head, mated 4-day male    24 head, mated 20-day male  26 salivary gland, larvae L3 wandering    6 salivary gland, white prepupae    10 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    7 digestive system, 4-day adult    5 digestive system, 20-day adult    5 fat body, larvae L3 wandering    7 fat body, white prepupae    8 fat body, pupae P8  29 carcass, larvae L3 wandering  (39) carcass, 1-day adult    10 carcass, 4-day adult    9 carcass, 20-day adult    10 ovary, virgin 4-day female    10 ovary, mated 4-day female    6 testis, mated 4-day male    7 accessory gland, mated 4-day male    16 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    47 central nervous system, larvae L3    17 central nervous system, pupae P8    28 head, virgin 1-day female    25 head, virgin 4-day female    17 head, virgin 20-day female    20 head, mated 1-day female    17 head, mated 4-day female    14 head, mated 20-day female    15 head, mated 1-day male    38 head, mated 4-day male    24 head, mated 20-day male    26 salivary gland, larvae L3 wandering    6 salivary gland, white prepupae    10 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    7 digestive system, 4-day adult    5 digestive system, 20-day adult    5 fat body, larvae L3 wandering    7 fat body, white prepupae    8 fat body, pupae P8    29 carcass, larvae L3 wandering    39 carcass, 1-day adult    10 carcass, 4-day adult    9 carcass, 20-day adult    10 ovary, virgin 4-day female    10 ovary, mated 4-day female    6 testis, mated 4-day male    7 accessory gland, mated 4-day male    16 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    47 central nervous system, larvae L3    17 central nervous system, pupae P8    28 head, virgin 1-day female    25 head, virgin 4-day female    17 head, virgin 20-day female    20 head, mated 1-day female    17 head, mated 4-day female    14 head, mated 20-day female    15 head, mated 1-day male    38 head, mated 4-day male    24 head, mated 20-day male    26 salivary gland, larvae L3 wandering    6 salivary gland, white prepupae    10 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    7 digestive system, 4-day adult    5 digestive system, 20-day adult    5 fat body, larvae L3 wandering    7 fat body, white prepupae    8 fat body, pupae P8    29 carcass, larvae L3 wandering    39 carcass, 1-day adult    10 carcass, 4-day adult    9 carcass, 20-day adult    10 ovary, virgin 4-day female    10 ovary, mated 4-day female    6 testis, mated 4-day male    7 accessory gland, mated 4-day male    16 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed within 06-18 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    8 embryo 02-04hr    14 embryo 04-06hr    4 embryo 06-08hr    24 embryo 08-10hr    61 embryo 10-12hr    270 embryo 12-14hr    157 embryo 14-16hr    152 embryo 16-18hr    130 embryo 18-20hr    113 embryo 20-22hr    64 embryo 22-24hr    62 larva L1    45 larva L2    32 larva L3 12hr old    21 larva L3 puffstage 1-2    23 larva L3 puffstage 3-6    34 larva L3 puffstage 7-9    39 white prepupae new    47 white prepupae 12hr    53 white prepupae 24hr    62 pupae 2d postWPP    58 pupae 3d postWPP    91 pupae 4d postWPP    55 adult male 01day    30 adult male 05day    22 adult male 30day    24 adult female 01day    25 adult female 05day    12 adult female 30day    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    8 embryo 02-04hr    14 embryo 04-06hr    4 embryo 06-08hr    24 embryo 08-10hr  (61) embryo 10-12hr  (270) embryo 12-14hr  (157) embryo 14-16hr  (152) embryo 16-18hr  (130) embryo 18-20hr  (113) embryo 20-22hr  (64) embryo 22-24hr  (62) larva L1  (45) larva L2  (32) larva L3 12hr old    21 larva L3 puffstage 1-2    23 larva L3 puffstage 3-6  (34) larva L3 puffstage 7-9  (39) white prepupae new  (47) white prepupae 12hr  (53) white prepupae 24hr  (62) pupae 2d postWPP  (58) pupae 3d postWPP  (91) pupae 4d postWPP  (55) adult male 01day  (30) adult male 05day    22 adult male 30day    24 adult female 01day    25 adult female 05day    12 adult female 30day    14 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    8 embryo 02-04hr    14 embryo 04-06hr    4 embryo 06-08hr    24 embryo 08-10hr    61 embryo 10-12hr  (270) embryo 12-14hr  (157) embryo 14-16hr  (152) embryo 16-18hr  (130) embryo 18-20hr  (113) embryo 20-22hr    64 embryo 22-24hr    62 larva L1    45 larva L2    32 larva L3 12hr old    21 larva L3 puffstage 1-2    23 larva L3 puffstage 3-6    34 larva L3 puffstage 7-9    39 white prepupae new    47 white prepupae 12hr    53 white prepupae 24hr    62 pupae 2d postWPP    58 pupae 3d postWPP    91 pupae 4d postWPP    55 adult male 01day    30 adult male 05day    22 adult male 30day    24 adult female 01day    25 adult female 05day    12 adult female 30day    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    8 embryo 02-04hr    14 embryo 04-06hr    4 embryo 06-08hr    24 embryo 08-10hr    61 embryo 10-12hr    270 embryo 12-14hr    157 embryo 14-16hr    152 embryo 16-18hr    130 embryo 18-20hr    113 embryo 20-22hr    64 embryo 22-24hr    62 larva L1    45 larva L2    32 larva L3 12hr old    21 larva L3 puffstage 1-2    23 larva L3 puffstage 3-6    34 larva L3 puffstage 7-9    39 white prepupae new    47 white prepupae 12hr    53 white prepupae 24hr    62 pupae 2d postWPP    58 pupae 3d postWPP    91 pupae 4d postWPP    55 adult male 01day    30 adult male 05day    22 adult male 30day    24 adult female 01day    25 adult female 05day    12 adult female 30day    14 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    8 embryo 02-04hr    14 embryo 04-06hr    4 embryo 06-08hr    24 embryo 08-10hr    61 embryo 10-12hr    270 embryo 12-14hr    157 embryo 14-16hr    152 embryo 16-18hr    130 embryo 18-20hr    113 embryo 20-22hr    64 embryo 22-24hr    62 larva L1    45 larva L2    32 larva L3 12hr old    21 larva L3 puffstage 1-2    23 larva L3 puffstage 3-6    34 larva L3 puffstage 7-9    39 white prepupae new    47 white prepupae 12hr    53 white prepupae 24hr    62 pupae 2d postWPP    58 pupae 3d postWPP    91 pupae 4d postWPP    55 adult male 01day    30 adult male 05day    22 adult male 30day    24 adult female 01day    25 adult female 05day    12 adult female 30day    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    8 embryo 02-04hr    14 embryo 04-06hr    4 embryo 06-08hr    24 embryo 08-10hr  (61) embryo 10-12hr  (270) embryo 12-14hr  (157) embryo 14-16hr  (152) embryo 16-18hr  (130) embryo 18-20hr  (113) embryo 20-22hr  (64) embryo 22-24hr  (62) larva L1  (45) larva L2  32 larva L3 12hr old    21 larva L3 puffstage 1-2    23 larva L3 puffstage 3-6  (34) larva L3 puffstage 7-9  (39) white prepupae new  (47) white prepupae 12hr  (53) white prepupae 24hr  (62) pupae 2d postWPP  (58) pupae 3d postWPP  (91) pupae 4d postWPP  (55) adult male 01day  30 adult male 05day    22 adult male 30day    24 adult female 01day    25 adult female 05day    12 adult female 30day    14 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    8 embryo 02-04hr    14 embryo 04-06hr    4 embryo 06-08hr    24 embryo 08-10hr    61 embryo 10-12hr  (270) embryo 12-14hr  (157) embryo 14-16hr  (152) embryo 16-18hr  130 embryo 18-20hr  113 embryo 20-22hr    64 embryo 22-24hr    62 larva L1    45 larva L2    32 larva L3 12hr old    21 larva L3 puffstage 1-2    23 larva L3 puffstage 3-6    34 larva L3 puffstage 7-9    39 white prepupae new    47 white prepupae 12hr    53 white prepupae 24hr    62 pupae 2d postWPP    58 pupae 3d postWPP    91 pupae 4d postWPP    55 adult male 01day    30 adult male 05day    22 adult male 30day    24 adult female 01day    25 adult female 05day    12 adult female 30day    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    8 embryo 02-04hr    14 embryo 04-06hr    4 embryo 06-08hr    24 embryo 08-10hr    61 embryo 10-12hr    270 embryo 12-14hr    157 embryo 14-16hr    152 embryo 16-18hr    130 embryo 18-20hr    113 embryo 20-22hr    64 embryo 22-24hr    62 larva L1    45 larva L2    32 larva L3 12hr old    21 larva L3 puffstage 1-2    23 larva L3 puffstage 3-6    34 larva L3 puffstage 7-9    39 white prepupae new    47 white prepupae 12hr    53 white prepupae 24hr    62 pupae 2d postWPP    58 pupae 3d postWPP    91 pupae 4d postWPP    55 adult male 01day    30 adult male 05day    22 adult male 30day    24 adult female 01day    25 adult female 05day    12 adult female 30day    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    6 Schneider line 2 Sg4    5 embryonic 1182-4H    6 embryonic GM2    9 embryonic Kc167    3 embryonic S1    11 embryonic S3    1 leg disc CME L1    7 wing disc CME-W2    10 wing disc ML-DmD8    9 wing disc ML-DmD9    18 wing disc ML-DmD16-c3    18 wing disc ML-DmD21    1 wing disc ML-DmD32    13 haltere disc ML-DmD17-c3    5 eye-antennal disc ML-DmD11    7 antennal disc ML-DmD20-c5    8 mixed discs ML-DmD4-c1    12 CNS ML-DmBG1-c1    17 CNS ML-DmBG2-c2    51 tumorous blood cells mbn2    8 ovary fGS/OSS    42 ovary OSC    6 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    6 Schneider line 2 Sg4    5 embryonic 1182-4H    6 embryonic GM2    9 embryonic Kc167    3 embryonic S1    11 embryonic S3    1 leg disc CME L1    7 wing disc CME-W2    10 wing disc ML-DmD8    9 wing disc ML-DmD9    18 wing disc ML-DmD16-c3    18 wing disc ML-DmD21    1 wing disc ML-DmD32    13 haltere disc ML-DmD17-c3    5 eye-antennal disc ML-DmD11    7 antennal disc ML-DmD20-c5    8 mixed discs ML-DmD4-c1    12 CNS ML-DmBG1-c1    17 CNS ML-DmBG2-c2  (51) tumorous blood cells mbn2    8 ovary fGS/OSS  (42) ovary OSC    6 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    6 Schneider line 2 Sg4    5 embryonic 1182-4H    6 embryonic GM2    9 embryonic Kc167    3 embryonic S1    11 embryonic S3    1 leg disc CME L1    7 wing disc CME-W2    10 wing disc ML-DmD8    9 wing disc ML-DmD9    18 wing disc ML-DmD16-c3    18 wing disc ML-DmD21    1 wing disc ML-DmD32    13 haltere disc ML-DmD17-c3    5 eye-antennal disc ML-DmD11    7 antennal disc ML-DmD20-c5    8 mixed discs ML-DmD4-c1    12 CNS ML-DmBG1-c1    17 CNS ML-DmBG2-c2    51 tumorous blood cells mbn2    8 ovary fGS/OSS    42 ovary OSC    6 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    6 Schneider line 2 Sg4    5 embryonic 1182-4H    6 embryonic GM2    9 embryonic Kc167    3 embryonic S1    11 embryonic S3    1 leg disc CME L1    7 wing disc CME-W2    10 wing disc ML-DmD8    9 wing disc ML-DmD9    18 wing disc ML-DmD16-c3    18 wing disc ML-DmD21    1 wing disc ML-DmD32    13 haltere disc ML-DmD17-c3    5 eye-antennal disc ML-DmD11    7 antennal disc ML-DmD20-c5    8 mixed discs ML-DmD4-c1    12 CNS ML-DmBG1-c1    17 CNS ML-DmBG2-c2    51 tumorous blood cells mbn2    8 ovary fGS/OSS    42 ovary OSC    6 ovary OSS    22 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    6 Schneider line 2 Sg4    5 embryonic 1182-4H    6 embryonic GM2    9 embryonic Kc167    3 embryonic S1    11 embryonic S3    1 leg disc CME L1    7 wing disc CME-W2    10 wing disc ML-DmD8    9 wing disc ML-DmD9    18 wing disc ML-DmD16-c3    18 wing disc ML-DmD21    1 wing disc ML-DmD32    13 haltere disc ML-DmD17-c3    5 eye-antennal disc ML-DmD11    7 antennal disc ML-DmD20-c5    8 mixed discs ML-DmD4-c1    12 CNS ML-DmBG1-c1    17 CNS ML-DmBG2-c2    51 tumorous blood cells mbn2    8 ovary fGS/OSS    42 ovary OSC    6 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    6 Schneider line 2 Sg4    5 embryonic 1182-4H    6 embryonic GM2    9 embryonic Kc167    3 embryonic S1    11 embryonic S3    1 leg disc CME L1    7 wing disc CME-W2    10 wing disc ML-DmD8    9 wing disc ML-DmD9    18 wing disc ML-DmD16-c3    18 wing disc ML-DmD21    1 wing disc ML-DmD32    13 haltere disc ML-DmD17-c3    5 eye-antennal disc ML-DmD11    7 antennal disc ML-DmD20-c5    8 mixed discs ML-DmD4-c1    12 CNS ML-DmBG1-c1    17 CNS ML-DmBG2-c2  (51) tumorous blood cells mbn2    8 ovary fGS/OSS  (42) ovary OSC    6 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    6 Schneider line 2 Sg4    5 embryonic 1182-4H    6 embryonic GM2    9 embryonic Kc167    3 embryonic S1    11 embryonic S3    1 leg disc CME L1    7 wing disc CME-W2    10 wing disc ML-DmD8    9 wing disc ML-DmD9    18 wing disc ML-DmD16-c3    18 wing disc ML-DmD21    1 wing disc ML-DmD32    13 haltere disc ML-DmD17-c3    5 eye-antennal disc ML-DmD11    7 antennal disc ML-DmD20-c5    8 mixed discs ML-DmD4-c1    12 CNS ML-DmBG1-c1    17 CNS ML-DmBG2-c2    51 tumorous blood cells mbn2    8 ovary fGS/OSS    42 ovary OSC    6 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    6 Schneider line 2 Sg4    5 embryonic 1182-4H    6 embryonic GM2    9 embryonic Kc167    3 embryonic S1    11 embryonic S3    1 leg disc CME L1    7 wing disc CME-W2    10 wing disc ML-DmD8    9 wing disc ML-DmD9    18 wing disc ML-DmD16-c3    18 wing disc ML-DmD21    1 wing disc ML-DmD32    13 haltere disc ML-DmD17-c3    5 eye-antennal disc ML-DmD11    7 antennal disc ML-DmD20-c5    8 mixed discs ML-DmD4-c1    12 CNS ML-DmBG1-c1    17 CNS ML-DmBG2-c2    51 tumorous blood cells mbn2    8 ovary fGS/OSS    42 ovary OSC    6 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    11 cold shock, 4-day adult    10 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    19 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    13 Cadmium 100 mM 48 hrs, 4-day adult    13 Copper 0.5 mM 12 hrs, larvae L3    15 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    17 Ethanol 10% 3 hrs, larvae L3    16 Caffeine 1.5 mg/ml 4 hrs, larvae L3    16 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    12 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    15 Paraquat 10 mM 48 hrs, 4-day adult    19 Rotenone 2 μg 12 hrs, larvae L3    14 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    11 cold shock, 4-day adult    10 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    19 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    13 Cadmium 100 mM 48 hrs, 4-day adult    13 Copper 0.5 mM 12 hrs, larvae L3    15 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    17 Ethanol 10% 3 hrs, larvae L3    16 Caffeine 1.5 mg/ml 4 hrs, larvae L3    16 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    12 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    15 Paraquat 10 mM 48 hrs, 4-day adult    19 Rotenone 2 μg 12 hrs, larvae L3    14 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    11 cold shock, 4-day adult    10 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    19 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    13 Cadmium 100 mM 48 hrs, 4-day adult    13 Copper 0.5 mM 12 hrs, larvae L3    15 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    17 Ethanol 10% 3 hrs, larvae L3    16 Caffeine 1.5 mg/ml 4 hrs, larvae L3    16 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    12 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    15 Paraquat 10 mM 48 hrs, 4-day adult    19 Rotenone 2 μg 12 hrs, larvae L3    14 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    11 cold shock, 4-day adult    10 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    19 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    13 Cadmium 100 mM 48 hrs, 4-day adult    13 Copper 0.5 mM 12 hrs, larvae L3    15 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    17 Ethanol 10% 3 hrs, larvae L3    16 Caffeine 1.5 mg/ml 4 hrs, larvae L3    16 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    12 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    15 Paraquat 10 mM 48 hrs, 4-day adult    19 Rotenone 2 μg 12 hrs, larvae L3    14 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    11 cold shock, 4-day adult    10 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    19 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    13 Cadmium 100 mM 48 hrs, 4-day adult    13 Copper 0.5 mM 12 hrs, larvae L3    15 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    17 Ethanol 10% 3 hrs, larvae L3    16 Caffeine 1.5 mg/ml 4 hrs, larvae L3    16 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    12 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    15 Paraquat 10 mM 48 hrs, 4-day adult    19 Rotenone 2 μg 12 hrs, larvae L3    14 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    11 cold shock, 4-day adult    10 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    19 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    13 Cadmium 100 mM 48 hrs, 4-day adult    13 Copper 0.5 mM 12 hrs, larvae L3    15 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    17 Ethanol 10% 3 hrs, larvae L3    16 Caffeine 1.5 mg/ml 4 hrs, larvae L3    16 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    12 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    15 Paraquat 10 mM 48 hrs, 4-day adult    19 Rotenone 2 μg 12 hrs, larvae L3    14 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    11 cold shock, 4-day adult    10 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    19 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    13 Cadmium 100 mM 48 hrs, 4-day adult    13 Copper 0.5 mM 12 hrs, larvae L3    15 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    17 Ethanol 10% 3 hrs, larvae L3    16 Caffeine 1.5 mg/ml 4 hrs, larvae L3    16 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    12 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    15 Paraquat 10 mM 48 hrs, 4-day adult    19 Rotenone 2 μg 12 hrs, larvae L3    14 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    11 cold shock, 4-day adult    10 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    19 Cadmium 50 mM 12 hrs, larvae L3    16 Cadmium 50 mM 48 hrs, 4-day adult    13 Cadmium 100 mM 48 hrs, 4-day adult    13 Copper 0.5 mM 12 hrs, larvae L3    15 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    17 Ethanol 10% 3 hrs, larvae L3    16 Caffeine 1.5 mg/ml 4 hrs, larvae L3    16 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    12 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    15 Paraquat 10 mM 48 hrs, 4-day adult    19 Rotenone 2 μg 12 hrs, larvae L3    14 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_29
External Data & Images
Linkouts	BDGP expression data • RH61449 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0250785 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG9326-RB FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for vari Stages(s) 1-3 Stages(s) 4-6 Stages(s) 7-8 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele cell lethal | somatic clone vari2L1623 vari2L3215 lethal (with Df(2L)Exel7079) vari48EP variB4 variB5 variK4 variL4 lethal (with Df(2L)TW2) vari38EFa2 vari327 vari03953b varif00033 variHM2043 variR3 variR979 variR2376 lethal (with vari38EFa2) vari327 varif00033 variR3 lethal (with vari48EP) variGE13049 lethal (with vari327) vari38EFa2 vari03953b varif00033 variHM2043 variR3 variR979 variR2376 lethal (with vari03953b) vari327 varif00033 variHM2043 variR3 variR979 variR2376 lethal (with variB4) variGE13049 lethal (with variB5) variGE13049 lethal (with varif00033) vari38EFa2 vari327 vari03953b variHM2043 variR3 variR979 variR2376 lethal (with variGE13049) vari48EP variB4 variB5 variK4 variL4 lethal (with variHM2043) vari327 vari03953b varif00033 lethal (with variK4) variGE13049 lethal (with variL4) variGE13049 lethal (with variR3) vari38EFa2 vari327 vari03953b varif00033 lethal (with variR979) vari327 vari03953b varif00033 lethal (with variR2376) vari327 vari03953b varif00033 lethal | embryonic stage vari48EP variB4 variB5 variGE13049 variK4 variL4 variMD109 lethal | embryonic stage (with vari03953b) variMD109 lethal | embryonic stage (with variMD109) vari03953b lethal | partially (with vari38EFa2) variHM2043 variR979 variR2376 lethal | partially (with variHM2043) vari38EFa2 lethal | partially (with variR979) vari38EFa2 lethal | partially (with variR2376) vari38EFa2 lethal | pharate adult stage, with Scer\GAL4sd-SG29.1 varidsRNA.Scer\UAS.cBa lethal | pharate adult stage P15(ii), with Scer\GAL4pnr-MD237 variGD13798 lethal | pupal stage, with Scer\GAL4pnr-MD237 variGD13798 lethal | recessive vari38EFa2 vari327 vari03953b varif00033 partially lethal - majority live vari38EFa2 partially lethal - majority live (with vari38EFa2) vari03953b partially lethal - majority live (with vari03953b) vari38EFa2 Other Phenotypes Allele visible, with Scer\GAL4da.G32 variScer\UAS.cMa visible, with Scer\GAL4dpp.PU variGD13798 visible, with Scer\GAL4ey.PH varidsRNA.Scer\UAS.cBa visible, with Scer\GAL4sd-SG29.1 varidsRNA.Scer\UAS.cBa Phenotype manifest in Allele dorsal trunk primordium vari48EP vari03953b varif00033 varidsRNA.cWa dorsal trunk primordium, with Scer\GAL4da.G32 variGD13798 dorsal trunk primordium (with vari48EP) vari03953b dorsal trunk primordium (with vari03953b) vari48EP embryonic/larval dorsal trunk vari03953b vari2L1623 vari2L3215 embryonic/larval tracheal system variMD109 vari03953b embryonic/larval tracheal system (with vari03953b) variMD109 embryonic/larval tracheal system (with variMD109) vari03953b embryonic/larval transverse connective vari03953b eye, with Scer\GAL4ey.PH varidsRNA.Scer\UAS.cBa ganglionic branch primordium varif00033 interommatidial bristle, with Scer\GAL4da.G32 variScer\UAS.cMa lateral trunk primordium vari48EP ocellus, with Scer\GAL4ey.PH varidsRNA.Scer\UAS.cBa pleated septate junction variMD109 vari03953b presumptive embryonic/larval tracheal system vari03953b varif00033 vari38EFa2 variR2376 variHM2043 variR979 variR3 vari327 septate junction vari03953b varif00033 variR2376 variHM2043 variR979 variR3 vari327 wing, with Scer\GAL4sd-SG29.1 varidsRNA.Scer\UAS.cBa wing hair, with Scer\GAL4dpp.PU variGD13798 wing margin, with Scer\GAL4sd-SG29.1 varidsRNA.Scer\UAS.cBa
Classical Alleles ( 23 )
For All Classical Alleles Show
Allele of vari Class Mutagen Stocks Known lesion vari38EFa2 ethyl methanesulfonate 2 Yes varif00033 loss of function allele 2 Yes variLA00256 2 -- vari48EP amorphic allele - genetic evidence P-element activity 1 Yes vari03953b loss of function allele P-element activity 1 Yes varid10880 P-element activity 1 -- varid11181 P-element activity 1 -- varie01914 piggyBac transposase 1 -- vari2L1623 ethyl methanesulfonate 0 -- vari2L3215 ethyl methanesulfonate 0 -- vari327 loss of function allele 0 Yes vari34P P-element activity 0 Yes variB4 P-element activity 0 Yes variB5 P-element activity 0 Yes varid11504 P-element activity 0 -- variGE13049 0 Yes variHM2043 0 Yes variK4 P-element activity 0 Yes variL4 P-element activity 0 Yes variMD109 P-element activity 0 Yes variR2376 0 Yes variR3 loss of function allele 0 Yes variR979 0 Yes
Alleles Carried on Transgenic Constructs ( 11 )
For All Alleles Carried on Transgenic Constructs Show
Allele of vari Class Mutagen Stocks Known lesion variGD13798 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes variScer\UAS.cMa 2 Yes variGL00065 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes variHM05087 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes variKK110006 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes varidsRNA.cWa in vitro construct - RNAi 0 Yes varidsRNA.Scer\UAS.cBa in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes varilong.Scer\UAS.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes varilong.Scer\UAS in vitro construct - regulatory fusion 0 Yes variNIG.5058R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes varishort.Scer\UAS in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)DS5 (Butler et al., 2001) Df(2L)DS6 (Bachmann et al., 2008, Kozlova et al., 1998) Df(2L)DS9 (Kozlova et al., 1998, Butler et al., 2001) Df(2L)Exel7079 (Ryder, 2004.4.26) Df(2L)pr1122 (Butler et al., 2001) Df(2L)TW2 (Butler et al., 2001) Df(2L)TW84 (Beitel and Krasnow, 2000) Df(2L)TW161 (Beitel and Krasnow, 2000) Df(2L)VA6 (Butler et al., 2001) Df(2L)vari48EP (Moyer and Jacobs, 2008)
Partially disrupted in	Df(2L)vari48EP (Moyer and Jacobs, 2008)
Not disrupted in	Df(2L)Acon-21 (Butler et al., 2001) Df(2L)DS8 (Butler et al., 2001) Df(2L)Fs(2)Ket-RX32 (Kozlova et al., 1998, Butler et al., 2001) Df(2L)pr11 (Butler et al., 2001)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{KK110006} NA P{NIG.5058R} NA P{TRiP.GL00065} NA P{TRiP.HM05087} NA P{UAS-FLAG-vari.long} NA P{UAS-vari.long} NA P{UAS-vari.M} NA P{UAS-vari.RNAi} NA P{UAS-vari.short} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}variGE13049 NA P{Mae-UAS.6.11}variLA00256 NA P{XP}varid10880 NA P{XP}varid11181 NA P{XP}varid11504 NA miscellaneous insertions P{?EP}vari48EP NA P{?EP}variB4 NA P{?EP}variB5 NA P{?XP}variMD109 NA PBac{RB}varie01914 NA PBac{WH}varif00033 NA
Gene Ontology: Function, Process & Cellular Component ( 9 unique terms )
Terms Based on Experimental Evidence ( 7 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
dorsal trunk growth, open tracheal system inferred from mutant phenotype (Baer et al., 2007) open tracheal system development inferred from mutant phenotype (Beitel and Krasnow, 2000, Moyer and Jacobs, 2008) regulation of tube length, open tracheal system inferred from mutant phenotype (Wu et al., 2007) regulation of tube size, open tracheal system inferred from mutant phenotype (Beitel and Krasnow, 2000) septate junction assembly inferred from mutant phenotype (Wu et al., 2007, Bachmann et al., 2008)
Cellular Component
CV term Evidence References
lateral plasma membrane inferred from direct assay (Moyer and Jacobs, 2008) septate junction inferred from direct assay (Wu et al., 2007, Bachmann et al., 2008)
Terms Based on Predictions or Assertions ( 2 terms )
Molecular Function
CV term Evidence References
guanylate kinase activity inferred from sequence or structural similarity with NCBI_gi:4885493 (FlyBase, 1992-)
Biological Process ( 0 terms)
Cellular Component
CV term Evidence References
membrane inferred from sequence or structural similarity with NCBI_gi:4885493 (FlyBase, 1992-)
Sequence Ontology: Class of Gene
	gene   nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions vari allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 61306 DroID - A comprehensive database of gene and protein interactions. • FBgn0250785 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35343 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • A1XXK3
Orthologs
OrthoDB Orthologs (97) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6J9NJF Dipteran inclusive ortholog search EOG6MPHZ8 Insect inclusive ortholog search EOG6ZGN7J Arthropod inclusive ortholog search EOG6T76JH Metazoa inclusive ortholog search EOG6W45F5
Orthologs in Drosophila Species (EOG6J9NJF)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\vari     Drosophila simulans   Dsim\GD21680 Y   Drosophila sechellia   Dsec\GM23306 Y   Drosophila erecta   Dere\GG21549 Y   Drosophila yakuba   Dyak\GE13543 Y   Drosophila ananassae   Dana\GF14547 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA21703 Y   Drosophila persimilis   Dper\GL19590 Y   Drosophila willistoni   Dwil\GK24864 Y   Drosophila virilis   Dvir\GJ20335 Y   Drosophila mojavensis   Dmoj\GI22442 Y   Drosophila grimshawi   Dgri\GH10158 Y
Orthologs in non-Drosophila Dipterans (EOG6MPHZ8)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL009937   Anopheles gambiae Malaria mosquito  Agam\AGAP008321   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ802426
Orthologs in non-Dipteran Insects (EOG6ZGN7J)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB45145   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG023752   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH10414   Atta cephalotes Leafcutter ant  Acep\ACEP23397   Camponotus floridanus Florida carpenter ant  Cflo\CFLO22813   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL19252   Linepithema humile Argentine ant  Lhum\LH18643   Pogonomyrmex barbatus Red harvester ant  Pbar\PB13444   Solenopsis invicta Red fire ant  Sinv\SINV10212   Acyrthosiphon pisum Pea aphid  Apim\ACYPI069428   Bombyx mori Silkmoth  Bmor\BGIBMGA001334   Pediculus humanus Human body louse  Phum\PHUM091990   Tribolium castaneum Red flour beetle  Tcas\TC014139
Orthologs in non-Insect Arthropods (EOG6T76JH)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\rpl36a
Orthologs in non-Arthropod Metazoa (EOG6W45F5)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\tag-117 Y Caenorhabditis elegans Nematode  Cele\Y55B1BR.4 Y Caenorhabditis elegans Nematode  Cele\lin-2 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_003310gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_016842gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_004864gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_003457gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_001995gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_016934gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_024853gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_028366gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_023432gn Y Danio rerio Zebrafish  Drer\mpp2a Y Danio rerio Zebrafish  Drer\ENSDARG00000025168 Y Danio rerio Zebrafish  Drer\ENSDARG00000035103 Y Danio rerio Zebrafish  Drer\mpp7 Y Danio rerio Zebrafish  Drer\ENSDARG00000062667 Y Danio rerio Zebrafish  Drer\OTTDARG00000031520 Y Danio rerio Zebrafish  Drer\mpp1 Y Danio rerio Zebrafish  Drer\mpp6b Y Danio rerio Zebrafish  Drer\mpp5b Y Danio rerio Zebrafish  Drer\mpp4a Y Danio rerio Zebrafish  Drer\mpp2b Y Danio rerio Zebrafish  Drer\ENSDARG00000015184 Y Danio rerio Zebrafish  Drer\mpp6a Y Danio rerio Zebrafish  Drer\cask Y Danio rerio Zebrafish  Drer\ENSDARG00000041883 Y Danio rerio Zebrafish  Drer\mpp5a Y Danio rerio Zebrafish  Drer\ENSDARG00000076872 Y Xenopus tropicalis Western clawed frog  Xtro\mpp1 Y Xenopus tropicalis Western clawed frog  Xtro\mpp6 Y Xenopus tropicalis Western clawed frog  Xtro\cask Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000031296 Y Xenopus tropicalis Western clawed frog  Xtro\mpp2 Y Xenopus tropicalis Western clawed frog  Xtro\mpp7 Y Xenopus tropicalis Western clawed frog  Xtro\mpp5 Y Gallus gallus Domestic chicken  Ggal\MPP1 Y Gallus gallus Domestic chicken  Ggal\MPP3 Y Gallus gallus Domestic chicken  Ggal\MPP4 Y Gallus gallus Domestic chicken  Ggal\MPP6 Y Gallus gallus Domestic chicken  Ggal\CASK Y Gallus gallus Domestic chicken  Ggal\MPP7 Y Gallus gallus Domestic chicken  Ggal\MPP5 Y Gallus gallus Domestic chicken  Ggal\MPP2 Y Mus musculus House mouse  Mmus\Mpp2 Y Mus musculus House mouse  Mmus\Mpp6 Y Mus musculus House mouse  Mmus\Mpp7 Y Mus musculus House mouse  Mmus\Mpp4 Y Mus musculus House mouse  Mmus\Mpp1 Y Mus musculus House mouse  Mmus\Mpp5 Y Mus musculus House mouse  Mmus\Mpp3 Y Mus musculus House mouse  Mmus\ENSMUSG00000031012 Y Rattus norvegicus Norway rat  Rnor\Cask Y Rattus norvegicus Norway rat  Rnor\Mpp3 Y Rattus norvegicus Norway rat  Rnor\Mpp5 Y Rattus norvegicus Norway rat  Rnor\Mpp6 Y Rattus norvegicus Norway rat  Rnor\Mpp4 Y Rattus norvegicus Norway rat  Rnor\Mpp2 Y Rattus norvegicus Norway rat  Rnor\Mpp7 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000038657 Y Homo sapiens Human  Hsap\MPP5 Y Homo sapiens Human  Hsap\MPP3 Y Homo sapiens Human  Hsap\MPP7 Y Homo sapiens Human  Hsap\MPP6 Y Homo sapiens Human  Hsap\MPP1 Y Homo sapiens Human  Hsap\ENSG00000108852 Y Homo sapiens Human  Hsap\MPP4 Y Homo sapiens Human  Hsap\ENSG00000147044 Y
Human Orthologs (8)
Gene OMIM HGNC Hsap\MPP5     Hsap\MPP3     Hsap\MPP7     Hsap\MPP6     Hsap\MPP1     Hsap\ENSG00000108852     Hsap\MPP4     Hsap\ENSG00000147044
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD21680 Drosophila sechellia Dsec\GM23306 Drosophila erecta Dere\GG21549 Drosophila yakuba Dyak\GE13543 Drosophila ananassae Dana\GF14547 Drosophila pseudoobscura pseudoobscura Dpse\GA21703 Drosophila persimilis Dper\GL19590 Drosophila willistoni Dwil\GK24864 Drosophila virilis Dvir\GJ20335 Drosophila mojavensis Dmoj\GI22442 Drosophila grimshawi Dgri\GH10158
Stocks & Reagents
Stocks Listed in FlyBase ( 17 )
Bloomington	36271 w*; P{UAS-vari.M}93 18289 w1118; PBac{WH}varif00033 5319 vari38EFa2 dpov1 bw1/CyO 35193 y1 sc* v1; P{TRiP.GL00065}attP2
Harvard	- P{XP}varid10880 - P{XP}varid11181 - PBac{RB}varie01914 - PBac{WH}varif00033
Kyoto	108174 vari38EFa2 dpov1 bw1/CyO
VDRC	v104548 P{KK110006}VIE-260B v24156 w1118 P{GD13798}v24156 v24157 w1118; P{GD13798}v24157
	More stocks available...
Genomic Clones ( 1 )
	BACR07D17 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 126 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE31492 RH61449
Other clones	FI01467 FI17352 MIP19750
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LD14962 LP08216 LP08920 RE02430 RE07489 RE09153 RE11052 RE12738 RE13216 RE13558 RE14774 RE16179 RE19253 RE20156 RE20664 RE23575 RE23587 RE25485 RE26632 RE30496 RE34028 RE34930 RE35569 RE35636 RE36020 RE39719 RE39732 RE40744 RE41541 RE42139 RE42140 RE42155 RE45072 RE46941 RE47555 RE47967 RE51859 RE51860 RE52159 RE54628 RE58272 RE60691 RE60702 RE61615 RE61782 RE62539 RE65750 RE67018 RE67030 RE67061 RE70342 RH13716 RH14941 RH42755 RH61149 RH61249 RH61549 SD26890
Other clones	10326 10740 32245 35489 37700 42381 60966 77903 101183 107328 160521 214569.7 CK01.20D.H8 CK01.61A.C7 EC054575 EC055812 EC056377 EK039120 EK053833 EK109551 EK110216 EK137206 EK142157 EK146352 EK149527 EK157312 EK173260 EK187916 EK210462 EK211741 EK223854 EK263345 EP12007 EP12243 ESG0115c.B21_B03 FGC024C04 FGM211C01 FGM215A08 RE01836 RM01007 RM01107 RM01207 RM01307 RM01707 RP01541 RP001018142 RP001022789 RP001041361 RP001053264 RP001054362 RP004045537 RP004137219 RP004176935 RP004198224 RP004231329 RP004245010 RP004293150 RP004328644 RP004467421 RP004476210 RP004503306 RP004535337 RP004556693
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0250785 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35343
Antibody Information
	polyclonal (Bachmann et al., 2008, Moyer and Jacobs, 2008)
Other Information
Discoverer
Etymology
	The gene is named "senz'aria", the Italian for "airless", as the trachea fail to inflate in mutant embryos. (Moyer and Jacobs, 2007)
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: szar CG9326 (Moyer and Jacobs, 2007) Source for identity of: vari l(2)03953 (Beitel and Krasnow, 2000)
Source for database merge of	Source for merge of: vari szar l(2)38EFa (Wu et al., 2007)
Additional comments
Other Comments
	vari is required for septate junction formation in the tracheal system. (Wu et al., 2007) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) Mutation in vari alters tracheal tube size in embryos without affecting other aspects of tracheal development including primary branch budding and outgrowth or branch fusion. (Beitel and Krasnow, 2000)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_165342 NM_165343 NM_206011 NM_001273715 NM_001273716 RefSeq (Proteins) • NP_724288 NP_724289 NP_995733 NP_001260644 NP_001260645 DDBJ / EMBL / GenBank • AA440379 AAF53925 AAF53925.2 AAF53925.3 AAL90354 AAM52036 AAN11089 AAN11089.1 AAN11089.2 AAN11090 AAN11090.1 AAN11090.2 ABH06960 ABH06961 ABH06962 ABH06963 ABH06964 ABH06965 ABH06966 ABH06967 AC004759 AGB93179 AGB93180 AY089616 AY121709 BI235604 BI631801 CZ477484 CZ477604 CZ477771 CZ479724 DQ787101 DQ787102 DQ787103 DQ787104 DQ787105 DQ787106 DQ787107 DQ787108 L80120 Entrez Gene - A searchable database of RefSeq genes. • 35343 UniProtKB/TrEMBL • A1XXK0 A1XXK1 A1XXK2 A1XXK3 A1XXK4 A1XXK5 A1XXK7 Q2PDR9 Q7KT16 Q8MR96 Q8SXI6 Q9VIJ4
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • P-loop containing nucleoside triphosphate hydrolase (IPR027417) L27, C-terminal (IPR014775) Variant SH3 (IPR011511) Guanylate kinase/L-type calcium channel (IPR008145) Guanylate kinase (IPR008144) L27 (IPR004172) PDZ domain (IPR001478) Src homology-3 domain (IPR001452) Guanylate kinase, conserved site (IPR020590) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • RH61449
Linkouts
	BioGRID - A database of protein and genetic interactions • 61306 DroID - A comprehensive database of gene and protein interactions. • FBgn0250785 DRSC - Results from RNAi screens. • FBgn0250785 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0250785 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG9326-RB FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0250785 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35343 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35343 modMine - Data generated by the modENCODE project. • FBgn0250785 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • A1XXK3
Synonyms & Secondary IDs ( 13 )
Reported As
Symbol Synonym	38E.21 (McGill Drosophila Genome Project, 2001.9.25, Butler, 2001, Butler et al., 2001) CG9326 (Wu et al., 2007, Moyer and Jacobs, 2007, Krejcí et al., 2009, Keleman et al., 2009.8.5, Perkins et al., 2009, Moyer and Jacobs, 2008, Tomancak et al., 2007, Ni et al., 2011.7.19, Bachmann et al., 2008) l(2)38EFa   l(2)03953 (Gelbart, 1999.7.20, BDGP Project Members, 1994-1999, Timmons et al., 1993, ) l(2)03953b (Beitel and Krasnow, 2000) szar (Moyer and Jacobs, 2007, Krejcí et al., 2009, Japanese National Institute of Genetics, 2012.5.21) vari (Swanson et al., 2009, Wu et al., 2007, Tonning et al., 2005, Bachmann et al., 2008, Laval et al., 2008, Moyer and Jacobs, 2008, Laprise et al., 2009) Vari (Laprise et al., 2010)
Name Synonym	CG9326   lethal (2) 38EFa   senz'aria (Moyer and Jacobs, 2007) senz-aria   varicose (Swanson et al., 2009, Beitel and Krasnow, 2000, Wu et al., 2007, Baer et al., 2007, Bachmann et al., 2008, Laprise et al., 2009) Varicose (Laval et al., 2008, Moyer and Jacobs, 2008, Laprise et al., 2010)
Secondary FlyBase IDs
	FBgn0010560 FBgn0025170 FBgn0032885 FBpp0088884 FBpp0088885 FBpp0088886 FBtr0089945 FBtr0089946 FBtr0089947
References ( 50 )
Generate a list of	All references relating to this gene ( 50 )
List References by type	Research paper  ( 22 ) Supplementary material  ( 5 ) Personal communication to FlyBase  ( 13 ) Abstract  ( 3 ) FlyBase analysis  ( 3 ) Stock list  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 3 )
	Juarez et al., 2011, PLoS Genet. 7(12): e1002424 Duox, Flotillin-2, and Src42A Are Required to Activate or Delimit the Spread of the Transcriptional Response to Epidermal Wounds in Drosophila. [FBrf0217196] Oshima and Fehon, 2011, J. Cell Sci. 124(16): 2861--2871 Analysis of protein dynamics within the septate junction reveals a highly stable core protein complex that does not include the basolateral polarity protein Discs large. [FBrf0214577] Rotstein et al., 2011, PLoS ONE 6(12): e28985 Tramtrack is genetically upstream of genes controlling tracheal tube size in Drosophila. [FBrf0217085] Show all research papers ...