Gene Dmel\β-Spec

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General Information

Symbol

Dmel\β-Spec

Species

D. melanogaster

Name

β Spectrin

Annotation symbol

CG5870

Feature type

protein_coding_gene

FlyBase ID

FBgn0250788

Gene Model Status

Current

Stock availability

10 publicly available

Also Known As

βspec, Spec, beta-Spec, l(1)G0074

Genomic Location

Chromosome (arm)

Recombination map

1-55 +/- 2

Cytogenetic map

16B10-16B12

Sequence location

X:17,551,454..17,563,270 [+]

Genomic Maps

Select View:

modENCODE GBrowse

Summary Information

Automatically generated summary

See sections below for more information

The gene β Spectrin is referred to in FlyBase by the symbol Dmel\β-Spec (CG5870, FBgn0250788). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: phosphatidylinositol-4,5-bisphosphate binding; microtubule binding; ankyrin binding. There is experimental evidence that it is involved in the biological process: negative regulation of microtubule depolymerization; neuromuscular synaptic transmission; regulation of synapse organization; long-term strengthening of neuromuscular junction; axon guidance; nervous system development; photoreceptor cell axon guidance; maintenance of presynaptic active zone structure; axonogenesis; axon midline choice point recognition. 31 alleles are reported. The phenotypes of these alleles are annotated with: organ system; multicellular structure; organ system subdivision; anatomical structure; synapse; portion of tissue; peripheral nervous system; cytoplasmic part; cell part; cell projection part; sense organ; postsynaptic membrane; adult segment. It has one annotated transcript and one annotated polypeptide. Protein features are: Actinin-type, actin-binding, conserved site; Calponin homology domain; Pleckstrin homology domain; Pleckstrin homology domain, spectrin-type; Pleckstrin homology-type; Spectrin repeat; Spectrin, beta subunit; Spectrin/alpha-actinin. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderately high expression. Peak expression observed within 06-24 hour embryonic stages, during early larval stages. Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, adult heart, larval/adult salivary gland, larval trachea. Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult Malpighian tubules, larval/adult fat body, adult female reproductive system, adult male reproductive system, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1636026_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of beta-Spec. Gene sequence location is X:17551454..17563270.

Phenotypic Description from the Red Book (Lindsley & Zimm 1992)

Gene/Allele symbols may differ from current usage

βSpec: beta Spectrin

Structural gene for beta spectrin; The subunit of beta spectrin associates with the subunit of alpha spectrin, forming heterodimers which associate to form tetramers, presumably the most important functional state for spectrin.

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2011_10

FB2012_01

Sequence features

BKN20825

HFA20311

All updates

Click here to see a list of all updates to this record from FB2010_08 and on.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

16B10-16B12

Limits computationally determined from genome sequence between P{EP}baz^EP1446 and P{EP}EP1464

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

16C1-16C2

(determined by in situ hybridisation) 16D1--2 (determined by in situ hybridisation)

(Peter et al., 2002)

16C1-16C2

(determined by in situ hybridisation)

(Schaefer et al., 1999.6.15)

16D1-16D2

(determined by in situ hybridisation)

(Schaefer et al., 1999.3.29)

16C1-16C4

(determined by in situ hybridisation)

(Byers et al., 1989)

Experimentally Determined Recombination Data

Location

1-55 +/- 2

(Hummel et al., 1999)

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\β-Spec for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

beta-Spec-RA

FBtr0074454

NM_078664

7488

2291

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

beta-Spec-PA

FBpp0074228

265.7

2291

4.99

NP_523388

AAF48751

Additional Polypeptide Data & Comments

Reported size (kDa)

2291 (aa); 235 (kD observed); 266 (kD predicted)

(Byers et al., 1992)

270 (kD)

(Dubreuil et al., 1990)

226 (kD)

(Dubreuil et al., 1987)

Comments

Spectrin subunits are dominated by tandemly repeated 106-residue motifs. A higher order 848 amino acid supra-motif composed of eight of the 106-residue motifs was observed in both α-Spec and β-Spec.

(Byers et al., 1992)

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Spectrin/alpha-actinin (IPR018159)

Pleckstrin homology-type (IPR011993)

Spectrin repeat (IPR002017)

Pleckstrin homology domain (IPR001849)

Calponin homology domain (IPR001715)

Pleckstrin homology domain, spectrin-type (IPR001605)

Actinin-type, actin-binding, conserved site (IPR001589)

Spectrin, beta subunit (IPR016343)

PDB - Protein Data Bank. An information portal to biological macromolecular structures

•

1DRO

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q00963

UniProtKB/TrEMBL

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

embryonic/larval somatic muscle

(Thomas and Kiehart, 1994)

epidermis

(Thomas and Kiehart, 1994)

embryonic/larval trachea

(Thomas and Kiehart, 1994)

mesoderm

(Thomas and Kiehart, 1994)

embryonic/larval visceral muscle

(Thomas and Kiehart, 1994)

embryonic stage 10

inclusive hindgut primordium

(Thomas and Kiehart, 1994)

embryonic/larval foregut | precursor

(Thomas and Kiehart, 1994)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Notes

Polypeptide Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage & oogenesis | early

spectrosome of germarium

(de Cuevas et al., 1996)

fusome of germarium

(de Cuevas et al., 1996)

adult stage & oogenesis

follicle cell

• plasma membrane | lateral

(Lee et al., 1997)

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage | late

embryonic/larval salivary gland

(Thomas and Kiehart, 1994)

amnioserosa

(Thomas and Kiehart, 1994)

embryonic stage | late & mid

embryonic/larval somatic muscle

(Thomas and Kiehart, 1994)

embryonic/larval esophagus

(Thomas and Kiehart, 1994)

embryonic stage | early

amnioproctodeal invagination

(Thomas and Kiehart, 1994)

ventral furrow

(Thomas and Kiehart, 1994)

cephalic furrow

(Thomas and Kiehart, 1994)

embryonic stage | mid

ventral midline

(Thomas and Kiehart, 1994)

embryonic stage | mid -- embryonic stage | late

embryonic/larval hindgut

(Thomas and Kiehart, 1994)

embryonic/larval trachea

(Thomas and Kiehart, 1994)

epidermis

(Thomas and Kiehart, 1994)

embryonic/larval visceral muscle

(Thomas and Kiehart, 1994)

pharyngeal muscle

(Thomas and Kiehart, 1994)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Maternally contributed β-Spec is uniformly distributed around the plasma membrane of the egg. During cellularization it is associated with the invaginating furrow canals and in a region of the lateral membranes at the apices of the forming cells. During gastrulation, the apicolateral staining remains and staining is added in a new apical cap in areas where morphogenetic movements occur including ventral and cephalic furrows and the posterior midgut invagination. Zygotic protein accumulates after ~stage 10 in the musculature, hindgut, epidermis, and trachea of the embryo as well as in other tissues.

(Dubreuil et al., 1997, Thomas and Kiehart, 1994)

Marker for

Subcellular Localization

CV Term

plasma membrane

(Thomas and Kiehart, 1994)

Notes

High-Throughput Expression Data

Untitled Document detailed view

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

modENCODE Temporal Expression Data for FBgn0250788

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	max expr for FBgn0250788
	Very low expression bin max
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderately high expression. Peak expression observed within 06-24 hour embryonic stages, during early larval stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No expression (0 - 0)
	Extremely low expression (1 - 10)
	Very low expression (11 - 100)
	Low expression (101 - 400)
	Moderate expression (401 - 1400)
	Moderately high expression (1401 - 4000)
	High expression (4001 - 10000)
	Very high expression (10001 - 100000)
	Extremely high expression (100001 - 2000000)

Linear, scaled to maximum FBgn0250788 expression level
Developmental Stage		Expression Level
embryo 00-02hr		1663
embryo 02-04hr		4773
embryo 04-06hr		8989
embryo 06-08hr		12779
embryo 08-10hr		14235
embryo 10-12hr		17021
embryo 12-14hr		14528
embryo 14-16hr		18107
embryo 16-18hr		14633
embryo 18-20hr		15353
embryo 20-22hr		13403
embryo 22-24hr		11566
larva L1		10207
larva L2		7964
larva L3 12hr old		6752
larva L3 puffstage 1-2		3599
larva L3 puffstage 3-6		4650
larva L3 puffstage 7-9		3639
white prepupae new		3890
white prepupae 12hr		5646
white prepupae 24hr		8353
pupae 2d postWPP		8342
pupae 3d postWPP		8720
pupae 4d postWPP		5792
adult male 01day		6535
adult male 05day		6448
adult male 30day		5772
adult female 01day		4659
adult female 05day		2514
adult female 30day		2720
Expression Level Scale		None Extremely low Very low Low Moderate Moderately high High Very high

Linear, scaled to Very low expression
Developmental Stage		Expression Level
embryo 00-02hr		(1663)
embryo 02-04hr		(4773)
embryo 04-06hr		(8989)
embryo 06-08hr		(12779)
embryo 08-10hr		(14235)
embryo 10-12hr		(17021)
embryo 12-14hr		(14528)
embryo 14-16hr		(18107)
embryo 16-18hr		(14633)
embryo 18-20hr		(15353)
embryo 20-22hr		(13403)
embryo 22-24hr		(11566)
larva L1		(10207)
larva L2		(7964)
larva L3 12hr old		(6752)
larva L3 puffstage 1-2		(3599)
larva L3 puffstage 3-6		(4650)
larva L3 puffstage 7-9		(3639)
white prepupae new		(3890)
white prepupae 12hr		(5646)
white prepupae 24hr		(8353)
pupae 2d postWPP		(8342)
pupae 3d postWPP		(8720)
pupae 4d postWPP		(5792)
adult male 01day		(6535)
adult male 05day		(6448)
adult male 30day		(5772)
adult female 01day		(4659)
adult female 05day		(2514)
adult female 30day		(2720)
Expression Level Scale		None Extremely low Very low Low

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(1663)
embryo 02-04hr		(4773)
embryo 04-06hr		(8989)
embryo 06-08hr		(12779)
embryo 08-10hr		(14235)
embryo 10-12hr		(17021)
embryo 12-14hr		(14528)
embryo 14-16hr		(18107)
embryo 16-18hr		(14633)
embryo 18-20hr		(15353)
embryo 20-22hr		(13403)
embryo 22-24hr		(11566)
larva L1		(10207)
larva L2		(7964)
larva L3 12hr old		(6752)
larva L3 puffstage 1-2		(3599)
larva L3 puffstage 3-6		(4650)
larva L3 puffstage 7-9		(3639)
white prepupae new		(3890)
white prepupae 12hr		(5646)
white prepupae 24hr		(8353)
pupae 2d postWPP		(8342)
pupae 3d postWPP		(8720)
pupae 4d postWPP		(5792)
adult male 01day		(6535)
adult male 05day		(6448)
adult male 30day		(5772)
adult female 01day		(4659)
adult female 05day		(2514)
adult female 30day		(2720)
Expression Level Scale		None Extremely low Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		1663
embryo 02-04hr		4773
embryo 04-06hr		8989
embryo 06-08hr		(12779)
embryo 08-10hr		(14235)
embryo 10-12hr		(17021)
embryo 12-14hr		(14528)
embryo 14-16hr		(18107)
embryo 16-18hr		(14633)
embryo 18-20hr		(15353)
embryo 20-22hr		(13403)
embryo 22-24hr		(11566)
larva L1		10207
larva L2		7964
larva L3 12hr old		6752
larva L3 puffstage 1-2		3599
larva L3 puffstage 3-6		4650
larva L3 puffstage 7-9		3639
white prepupae new		3890
white prepupae 12hr		5646
white prepupae 24hr		8353
pupae 2d postWPP		8342
pupae 3d postWPP		8720
pupae 4d postWPP		5792
adult male 01day		6535
adult male 05day		6448
adult male 30day		5772
adult female 01day		4659
adult female 05day		2514
adult female 30day		2720
Expression Level Scale		None Extremely low Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		1663
embryo 02-04hr		4773
embryo 04-06hr		8989
embryo 06-08hr		12779
embryo 08-10hr		14235
embryo 10-12hr		17021
embryo 12-14hr		14528
embryo 14-16hr		18107
embryo 16-18hr		14633
embryo 18-20hr		15353
embryo 20-22hr		13403
embryo 22-24hr		11566
larva L1		10207
larva L2		7964
larva L3 12hr old		6752
larva L3 puffstage 1-2		3599
larva L3 puffstage 3-6		4650
larva L3 puffstage 7-9		3639
white prepupae new		3890
white prepupae 12hr		5646
white prepupae 24hr		8353
pupae 2d postWPP		8342
pupae 3d postWPP		8720
pupae 4d postWPP		5792
adult male 01day		6535
adult male 05day		6448
adult male 30day		5772
adult female 01day		4659
adult female 05day		2514
adult female 30day		2720
Expression Level Scale		None Extremely low Very low Low Moderate Moderately high High Very high Extremely high

log, scaled to maximum FBgn0250788 expression level
Developmental Stage		Expression Level
embryo 00-02hr		1663
embryo 02-04hr		4773
embryo 04-06hr		8989
embryo 06-08hr		12779
embryo 08-10hr		14235
embryo 10-12hr		17021
embryo 12-14hr		14528
embryo 14-16hr		18107
embryo 16-18hr		14633
embryo 18-20hr		15353
embryo 20-22hr		13403
embryo 22-24hr		11566
larva L1		10207
larva L2		7964
larva L3 12hr old		6752
larva L3 puffstage 1-2		3599
larva L3 puffstage 3-6		4650
larva L3 puffstage 7-9		3639
white prepupae new		3890
white prepupae 12hr		5646
white prepupae 24hr		8353
pupae 2d postWPP		8342
pupae 3d postWPP		8720
pupae 4d postWPP		5792
adult male 01day		6535
adult male 05day		6448
adult male 30day		5772
adult female 01day		4659
adult female 05day		2514
adult female 30day		2720
Expression Level Scale		None Extremely low Very low Low Moderate Moderately high High Very high

log, scaled to Very low expression
Developmental Stage		Expression Level
embryo 00-02hr		(1663)
embryo 02-04hr		(4773)
embryo 04-06hr		(8989)
embryo 06-08hr		(12779)
embryo 08-10hr		(14235)
embryo 10-12hr		(17021)
embryo 12-14hr		(14528)
embryo 14-16hr		(18107)
embryo 16-18hr		(14633)
embryo 18-20hr		(15353)
embryo 20-22hr		(13403)
embryo 22-24hr		(11566)
larva L1		(10207)
larva L2		(7964)
larva L3 12hr old		(6752)
larva L3 puffstage 1-2		(3599)
larva L3 puffstage 3-6		(4650)
larva L3 puffstage 7-9		(3639)
white prepupae new		(3890)
white prepupae 12hr		(5646)
white prepupae 24hr		(8353)
pupae 2d postWPP		(8342)
pupae 3d postWPP		(8720)
pupae 4d postWPP		(5792)
adult male 01day		(6535)
adult male 05day		(6448)
adult male 30day		(5772)
adult female 01day		(4659)
adult female 05day		(2514)
adult female 30day		(2720)
Expression Level Scale		None Extremely low Very low Low

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		1663
embryo 02-04hr		(4773)
embryo 04-06hr		(8989)
embryo 06-08hr		(12779)
embryo 08-10hr		(14235)
embryo 10-12hr		(17021)
embryo 12-14hr		(14528)
embryo 14-16hr		(18107)
embryo 16-18hr		(14633)
embryo 18-20hr		(15353)
embryo 20-22hr		(13403)
embryo 22-24hr		(11566)
larva L1		(10207)
larva L2		(7964)
larva L3 12hr old		(6752)
larva L3 puffstage 1-2		(3599)
larva L3 puffstage 3-6		(4650)
larva L3 puffstage 7-9		(3639)
white prepupae new		(3890)
white prepupae 12hr		(5646)
white prepupae 24hr		(8353)
pupae 2d postWPP		(8342)
pupae 3d postWPP		(8720)
pupae 4d postWPP		(5792)
adult male 01day		(6535)
adult male 05day		(6448)
adult male 30day		(5772)
adult female 01day		(4659)
adult female 05day		(2514)
adult female 30day		(2720)
Expression Level Scale		None Extremely low Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		1663
embryo 02-04hr		4773
embryo 04-06hr		8989
embryo 06-08hr		12779
embryo 08-10hr		14235
embryo 10-12hr		17021
embryo 12-14hr		14528
embryo 14-16hr		18107
embryo 16-18hr		14633
embryo 18-20hr		15353
embryo 20-22hr		13403
embryo 22-24hr		11566
larva L1		10207
larva L2		7964
larva L3 12hr old		6752
larva L3 puffstage 1-2		3599
larva L3 puffstage 3-6		4650
larva L3 puffstage 7-9		3639
white prepupae new		3890
white prepupae 12hr		5646
white prepupae 24hr		8353
pupae 2d postWPP		8342
pupae 3d postWPP		8720
pupae 4d postWPP		5792
adult male 01day		6535
adult male 05day		6448
adult male 30day		5772
adult female 01day		4659
adult female 05day		2514
adult female 30day		2720
Expression Level Scale		None Extremely low Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		1663
embryo 02-04hr		4773
embryo 04-06hr		8989
embryo 06-08hr		12779
embryo 08-10hr		14235
embryo 10-12hr		17021
embryo 12-14hr		14528
embryo 14-16hr		18107
embryo 16-18hr		14633
embryo 18-20hr		15353
embryo 20-22hr		13403
embryo 22-24hr		11566
larva L1		10207
larva L2		7964
larva L3 12hr old		6752
larva L3 puffstage 1-2		3599
larva L3 puffstage 3-6		4650
larva L3 puffstage 7-9		3639
white prepupae new		3890
white prepupae 12hr		5646
white prepupae 24hr		8353
pupae 2d postWPP		8342
pupae 3d postWPP		8720
pupae 4d postWPP		5792
adult male 01day		6535
adult male 05day		6448
adult male 30day		5772
adult female 01day		4659
adult female 05day		2514
adult female 30day		2720
Expression Level Scale		None Extremely low Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

FlyAtlas Anatomical Expression Data for FBgn0250788

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	max expr for FBgn0250788
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, adult heart, larval/adult salivary gland, larval trachea. Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult Malpighian tubules, larval/adult fat body, adult female reproductive system, adult male reproductive system, larval/adult carcass.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (1000 - 25000)

Linear, scaled to maximum FBgn0250788 expression level
Tissue		Expression Level
Larval Central Nervous System		803.8
Larval Midgut		618.5
Larval Hindgut		902.3
Larval Malpighian Tubules		288.2
Larval Fat Body		121.1
Larval Salivary Gland		522.7
Larval Trachea		1013.4
Larval Carcass		489.875
Adult Head		508.7
Adult Eye		635.625
Adult Brain		991.5
Adult Thoracic-Abdominal Ganglion		1314.3
Adult Crop		788
Adult Midgut		643.7
Adult Hindgut		1014.8
Adult Malpighian Tubules		258.9
Adult Fat Body		295.8
Adult Salivary Gland		644.6
Adult Heart		626.875
Adult VirginFemale Spermatheca		234.7
Adult InseminatedFemale Spermatheca		275.7
Adult Ovary		103.6
Adult Testis		188.5
Adult Male Accessory Gland		335.4
Adult Carcass		399.2
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		(803.8)
Larval Midgut		(618.5)
Larval Hindgut		(902.3)
Larval Malpighian Tubules		288.2
Larval Fat Body		121.1
Larval Salivary Gland		522.7
Larval Trachea		(1013.4)
Larval Carcass		489.875
Adult Head		508.7
Adult Eye		(635.625)
Adult Brain		(991.5)
Adult Thoracic-Abdominal Ganglion		(1314.3)
Adult Crop		(788)
Adult Midgut		(643.7)
Adult Hindgut		(1014.8)
Adult Malpighian Tubules		258.9
Adult Fat Body		295.8
Adult Salivary Gland		(644.6)
Adult Heart		(626.875)
Adult VirginFemale Spermatheca		234.7
Adult InseminatedFemale Spermatheca		275.7
Adult Ovary		103.6
Adult Testis		188.5
Adult Male Accessory Gland		335.4
Adult Carcass		399.2
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		803.8
Larval Midgut		618.5
Larval Hindgut		902.3
Larval Malpighian Tubules		288.2
Larval Fat Body		121.1
Larval Salivary Gland		522.7
Larval Trachea		1013.4
Larval Carcass		489.875
Adult Head		508.7
Adult Eye		635.625
Adult Brain		991.5
Adult Thoracic-Abdominal Ganglion		(1314.3)
Adult Crop		788
Adult Midgut		643.7
Adult Hindgut		1014.8
Adult Malpighian Tubules		258.9
Adult Fat Body		295.8
Adult Salivary Gland		644.6
Adult Heart		626.875
Adult VirginFemale Spermatheca		234.7
Adult InseminatedFemale Spermatheca		275.7
Adult Ovary		103.6
Adult Testis		188.5
Adult Male Accessory Gland		335.4
Adult Carcass		399.2
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		803.8
Larval Midgut		618.5
Larval Hindgut		902.3
Larval Malpighian Tubules		288.2
Larval Fat Body		121.1
Larval Salivary Gland		522.7
Larval Trachea		1013.4
Larval Carcass		489.875
Adult Head		508.7
Adult Eye		635.625
Adult Brain		991.5
Adult Thoracic-Abdominal Ganglion		1314.3
Adult Crop		788
Adult Midgut		643.7
Adult Hindgut		1014.8
Adult Malpighian Tubules		258.9
Adult Fat Body		295.8
Adult Salivary Gland		644.6
Adult Heart		626.875
Adult VirginFemale Spermatheca		234.7
Adult InseminatedFemale Spermatheca		275.7
Adult Ovary		103.6
Adult Testis		188.5
Adult Male Accessory Gland		335.4
Adult Carcass		399.2
Expression Level Scale		None Low Moderate High Very high

log, scaled to maximum FBgn0250788 expression level
Tissue		Expression Level
Larval Central Nervous System		803.8
Larval Midgut		618.5
Larval Hindgut		902.3
Larval Malpighian Tubules		288.2
Larval Fat Body		121.1
Larval Salivary Gland		522.7
Larval Trachea		1013.4
Larval Carcass		489.875
Adult Head		508.7
Adult Eye		635.625
Adult Brain		991.5
Adult Thoracic-Abdominal Ganglion		1314.3
Adult Crop		788
Adult Midgut		643.7
Adult Hindgut		1014.8
Adult Malpighian Tubules		258.9
Adult Fat Body		295.8
Adult Salivary Gland		644.6
Adult Heart		626.875
Adult VirginFemale Spermatheca		234.7
Adult InseminatedFemale Spermatheca		275.7
Adult Ovary		103.6
Adult Testis		188.5
Adult Male Accessory Gland		335.4
Adult Carcass		399.2
Expression Level Scale		None Low Moderate High Very high

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		803.8
Larval Midgut		618.5
Larval Hindgut		(902.3)
Larval Malpighian Tubules		288.2
Larval Fat Body		121.1
Larval Salivary Gland		522.7
Larval Trachea		(1013.4)
Larval Carcass		489.875
Adult Head		508.7
Adult Eye		635.625
Adult Brain		(991.5)
Adult Thoracic-Abdominal Ganglion		(1314.3)
Adult Crop		788
Adult Midgut		643.7
Adult Hindgut		(1014.8)
Adult Malpighian Tubules		258.9
Adult Fat Body		295.8
Adult Salivary Gland		644.6
Adult Heart		626.875
Adult VirginFemale Spermatheca		234.7
Adult InseminatedFemale Spermatheca		275.7
Adult Ovary		103.6
Adult Testis		188.5
Adult Male Accessory Gland		335.4
Adult Carcass		399.2
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		803.8
Larval Midgut		618.5
Larval Hindgut		902.3
Larval Malpighian Tubules		288.2
Larval Fat Body		121.1
Larval Salivary Gland		522.7
Larval Trachea		1013.4
Larval Carcass		489.875
Adult Head		508.7
Adult Eye		635.625
Adult Brain		991.5
Adult Thoracic-Abdominal Ganglion		1314.3
Adult Crop		788
Adult Midgut		643.7
Adult Hindgut		1014.8
Adult Malpighian Tubules		258.9
Adult Fat Body		295.8
Adult Salivary Gland		644.6
Adult Heart		626.875
Adult VirginFemale Spermatheca		234.7
Adult InseminatedFemale Spermatheca		275.7
Adult Ovary		103.6
Adult Testis		188.5
Adult Male Accessory Gland		335.4
Adult Carcass		399.2
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		803.8
Larval Midgut		618.5
Larval Hindgut		902.3
Larval Malpighian Tubules		288.2
Larval Fat Body		121.1
Larval Salivary Gland		522.7
Larval Trachea		1013.4
Larval Carcass		489.875
Adult Head		508.7
Adult Eye		635.625
Adult Brain		991.5
Adult Thoracic-Abdominal Ganglion		1314.3
Adult Crop		788
Adult Midgut		643.7
Adult Hindgut		1014.8
Adult Malpighian Tubules		258.9
Adult Fat Body		295.8
Adult Salivary Gland		644.6
Adult Heart		626.875
Adult VirginFemale Spermatheca		234.7
Adult InseminatedFemale Spermatheca		275.7
Adult Ovary		103.6
Adult Testis		188.5
Adult Male Accessory Gland		335.4
Adult Carcass		399.2
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	508.7
NA	Eye	635.625
NA	Brain	991.5
803.8	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	1314.3
NA	Crop	788
618.5	Midgut	643.7
902.3	Hindgut	1014.8
288.2	Malpighian Tubules	258.9
121.1	Fat Body	295.8
522.7	Salivary Gland	644.6
NA	Heart	626.875
1013.4	Trachea	NA
NA	VirginFemale Spermatheca	234.7
NA	InseminatedFemale Spermatheca	275.7
NA	Ovary	103.6
NA	Testis	188.5
NA	Male Accessory Gland	335.4
489.875	Carcass	399.2

modENCODE Temporal Expression Data (Graveley et al., 2011)
FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010)

mE2_34_mRNA_expression_cluster_11

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0250788

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal

β-Spec^em6

lethal | embryonic stage

β-Spec^G0108

lethal | embryonic stage | recessive

lethal | first instar larval stage, with Scer\GAL4^da.G32

β-Spec^{dsRNA.Scer\UAS}

lethal | larval stage

β-Spec^G0198

lethal | larval stage, with Scer\GAL4^bab1-Agal4-5

β-Spec^{M.Scer\UAS.T:Hsap\MYC}

lethal | larval stage, with Scer\GAL4^Cg.PA

β-Spec^{M.Scer\UAS.T:Hsap\MYC}

lethal | larval stage, with Scer\GAL4^how-24B

β-Spec^{M.Scer\UAS.T:Hsap\MYC}

lethal | larval stage, with Scer\GAL4^mex1.2.1

β-Spec^{M.Scer\UAS.T:Hsap\MYC}

lethal | larval stage, with Scer\GAL4^phm.PO

β-Spec^{M.Scer\UAS.T:Hsap\MYC}

lethal | larval stage, with Scer\GAL4^αTub84B.PL

lethal | pharate adult stage

lethal | prepupal stage

β-Spec^G0198

lethal | pupal stage

β-Spec^G0198

lethal | recessive

lethal | third instar larval stage, with Scer\GAL4^sca-537.4

β-Spec^{dsRNA.Scer\UAS}

semi-lethal | recessive

β-Spec^G0108

viable, with Scer\GAL4^pnr-MD237

β-Spec^GD11790

Sterility

Allele

sterile, with β-Spec^em21

β-Spec^{ΔPH.Ubi-p63E.T:Hsap\MYC}

sterile, with β-Spec^{ΔPH.Ubi-p63E.T:Hsap\MYC}

β-Spec^em21

sterile (with β-Spec^em21)

β-Spec^{ΔPH.Ubi-p63E.T:Hsap\MYC}

sterile (with β-Spec^{ΔPH.Ubi-p63E.T:Hsap\MYC})

β-Spec^em21

Other Phenotypes

Allele

cell polarity defective, with Scer\GAL4^hs.PB

β-Spec^{M.Scer\UAS.T:Hsap\MYC}

cell shape defective, with Scer\GAL4^hs.PB

β-Spec^{M.Scer\UAS.T:Hsap\MYC}

hypoactive | recessive

neuroanatomy defective

neuroanatomy defective, with Scer\GAL4^elav.PLu

β-Spec^{dsRNA.Scer\UAS}

neuroanatomy defective, with Scer\GAL4^sca-537.4

β-Spec^{dsRNA.Scer\UAS}

neuroanatomy defective | somatic clone

β-Spec^G113

neurophysiology defective

neurophysiology defective, with Scer\GAL4^sca-537.4

β-Spec^{dsRNA.Scer\UAS}

short lived

β-Spec^em6

small body, with β-Spec^em21

β-Spec^{ΔPH.Ubi-p63E.T:Hsap\MYC}

small body, with β-Spec^{ΔPH.Ubi-p63E.T:Hsap\MYC}

β-Spec^em21

small body (with β-Spec^em21)

β-Spec^{ΔPH.Ubi-p63E.T:Hsap\MYC}

small body (with β-Spec^{ΔPH.Ubi-p63E.T:Hsap\MYC})

β-Spec^em21

wild-type

β-Spec^{Ubi-p63E.T:Hsap\MYC}

Phenotype manifest in

Allele

anterior commissure

β-Spec^em6

bouton, with Scer\GAL4^sca-537.4

β-Spec^{dsRNA.Scer\UAS}

cuprophilic cell

β-Spec^em6

embryonic/larval midgut | embryonic stage

embryonic/larval neuromuscular junction, with Scer\GAL4^elav.PLu

β-Spec^{dsRNA.Scer\UAS}

growth cone

β-Spec^unspecified

hub cell

β-Spec^em6

longitudinal connective

macrochaeta, with Scer\GAL4^rn-GAL4-14

β-Spec^{M.Scer\UAS.T:Hsap\MYC}

medial longitudinal fascicle | ectopic

medulla | somatic clone

β-Spec^G113

microtubule cytoskeleton & axon, with Scer\GAL4^sca-537.4

β-Spec^{dsRNA.Scer\UAS}

NMJ bouton, with Scer\GAL4^elav.PLu

β-Spec^{dsRNA.Scer\UAS}

posterior commissure

β-Spec^em6

postsynaptic membrane, with Scer\GAL4^sca-537.4

β-Spec^{dsRNA.Scer\UAS}

pupa

β-Spec^G0198

synapse

synapse, with Scer\GAL4^sca-537.4

β-Spec^{dsRNA.Scer\UAS}

synaptic vesicle, with Scer\GAL4^sca-537.4

β-Spec^{dsRNA.Scer\UAS}

third instar larva

β-Spec^G0198

ventral nerve cord commissure

β-Spec^S012

wing, with Scer\GAL4^2-21

β-Spec^{M.Scer\UAS.T:Hsap\MYC}

wing, with Scer\GAL4^Bx-MS1096

β-Spec^{M.Scer\UAS.T:Hsap\MYC}

wing, with Scer\GAL4^elav-C155

β-Spec^{M.Scer\UAS.T:Hsap\MYC}

wing, with Scer\GAL4^rn-GAL4-14

β-Spec^{M.Scer\UAS.T:Hsap\MYC}

Classical Alleles ( 23 )

For All Classical Alleles Show

Allele of β-Spec	Class	Mutagen	Stocks	Known lesion
β-Spec^G0074	hypomorphic allele - genetic evidence	P-element activity	2	--
β-Spec^G0198	hypomorphic allele - genetic evidence	P-element activity	2	--
β-Spec^KG02312		P-element activity	2	--
β-Spec^5-HA-2129			1	--
β-Spec^G0108	hypomorphic allele - genetic evidence	P-element activity	1	--
β-Spec^CB-6967-3			0	--
β-Spec^d03062		P-element activity	0	--
β-Spec^E175		ethyl methanesulfonate	0	--
β-Spec^E292	hypomorphic allele - genetic evidence	ethyl methanesulfonate	0	--
β-Spec^em12		ethyl methanesulfonate	0	Yes
β-Spec^em15		ethyl methanesulfonate	0	--
β-Spec^em21		ethyl methanesulfonate	0	Yes
β-Spec^em6		ethyl methanesulfonate	0	--
β-Spec^emb			0	--
β-Spec^G113	hypomorphic allele - genetic evidence	ethyl methanesulfonate	0	--
β-Spec^GG01075			0	--
β-Spec^H127		ethyl methanesulfonate	0	--
β-Spec^L105		ethyl methanesulfonate	0	--
β-Spec^M046		ethyl methanesulfonate	0	--
β-Spec^PG131		Delta2-3	0	--
β-Spec^PG96		Delta2-3	0	--
β-Spec^S012		ethyl methanesulfonate	0	Yes
β-Spec^unspecified			0	--

Alleles Carried on Transgenic Constructs ( 8 )

For All Alleles Carried on Transgenic Constructs Show

Allele of β-Spec	Mutagen	Stocks	Known lesion
β-Spec^GD11790	in vitro construct - RNAi in vitro construct - regulatory fusion	2	Yes
β-Spec^{dsRNA.Scer\UAS}	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes
β-Spec^{M.Scer\UAS.T:Hsap\MYC}	in vitro construct - regulatory fusion	0	Yes
β-Spec^Scer\UAS.cHa	in vitro construct - regulatory fusion	0	Yes
β-Spec^{Scer\UAS.T:Hsap\MYC}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	0	Yes
β-Spec^{Ubi-p63E.T:Hsap\MYC}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	0	Yes
β-Spec^{W2033R.Ubi-p63E.T:Hsap\MYC}	in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement in vitro construct - site directed mutagenesis	0	Yes
β-Spec^{ΔPH.Ubi-p63E.T:Hsap\MYC}	in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - site directed mutagenesis	0	Yes

Aneuploid Aberrations

Disrupted in

Df(1)SD10

(Dubreuil et al., 2000)

Duplicated in

Dp(1;3)B^S3iD2

(Dubreuil et al., 2000, Hulsmeier et al., 2007)

Dp(1;3)B^S3iD3

(Dubreuil et al., 2000)

Not duplicated in

Dp(1;3)B^S3iD1

(Dubreuil et al., 2000)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

characterization construct

P{Ubi-p63E-α-Spec::β-Spec.α8}

P{Ubi-p63E-β-Spec.myc}

P{β-Spec::Actn.ABD}

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{Mae-UAS.6.11}β-Spec^GG01075

P{XP}β-Spec^d03062

miscellaneous insertions

P{RS3}β-Spec^CB-6967-3

P{RS5}β-Spec^5-HA-2129

P{SUPor-P}β-Spec^KG02312

insertion of enhancer trap binary system

P{GawB}β-Spec^PG96

P{GawB}β-Spec^PG131

insertion of enhancer trap

P{lacW}β-Spec^G0074

P{lacW}β-Spec^G0108

P{lacW}β-Spec^G0198

Gene Ontology: Function, Process & Cellular Component ( 30 unique terms )

Terms Based on Experimental Evidence ( 20 terms )

Molecular Function

CV term

Evidence

References

ankyrin binding

inferred from direct assay

(Mazock et al., 2010)

microtubule binding

inferred from direct assay

(Sisson et al., 2000)

phosphatidylinositol-4,5-bisphosphate binding

inferred from direct assay

(Das et al., 2008)

Biological Process

CV term

Evidence

References

axon guidance