General Information Symbol Dmel\ gishSpecies D. melanogaster Name gilgamesh Annotation symbol CG6963 Feature type protein_coding_gene FlyBase ID FBgn0250823 Gene Model Status Current Stock availability 36 publicly
available Also Known As ms(3)89B, CKIγ, Hrr25, CK1γ Genomic Location Chromosome (arm) 3R Recombination map Cytogenetic map 89B9-89B12 Sequence location 3R:12,098,174..12,130,490 [+]
Genomic Maps
Summary Information Automatically generated summary
See sections below for more information The gene
gilgamesh is referred to in FlyBase by the symbol
Dmel\gish (CG6963, FBgn0250823). It is a protein_coding_gene from
Drosophila melanogaster . Based on sequence similarity, it is predicted to have
molecular function : protein serine/threonine kinase activity. There is experimental evidence for 11 unique
biological process terms, many of which group under: biological regulation; regulation of developmental process; multicellular organism reproduction; cellular process; gamete generation; single-organism developmental process; regulation of cellular component organization; spermatid differentiation; macromolecule modification; spermatogenesis; localization; associative learning; ommatidial rotation; response to stimulus.
62 alleles are reported . The
phenotypes of these alleles are annotated with: spermatogonium; onion stage spermatid; investment cone; eye; trichogen cell; photoreceptor cell; ommatidium; mature eight-cell ommatidial precursor cluster; minor mitochondrial derivative. It has
13 annotated transcripts and
13 annotated polypeptides .
Protein features are: Casein kinase 1 gamma C-terminal; Protein kinase, ATP binding site; Protein kinase, catalytic domain; Protein kinase-like domain; Serine/threonine-protein kinase, active site. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Many larval and adult organs/tissues expressed at moderate or high levels. Expression at high levels in the following post-embryonic organs or tissues: adult eye. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval salivary gland, larval trachea, adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1626805_s_at completely aligns to an exonic region of only a subset of 8 of the 9 FlyBase-annotated transcript isoforms of gish. Gene sequence location is
3R:12098174..12130490 .
User Contributed Data
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FB2013_03
Alleles
FB2013_02
All updates
Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Experimentally Determined Cytological Location
89B1-89B22
(determined by in situ hybridisation)
Experimentally Determined Recombination Data
Location Left of (cM) Right of (cM) Notes Gene Model & Products Comments on Gene
Model gene_with_stop_codon_read_through ; SO:0000697
Gene model reviewed during 5.44
Stop-codon suppression (UGA) postulated; FBrf0216884.
Gene model reviewed during 5.47
Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Low-frequency RNA-Seq exon junction(s) not annotated.
Transcript Data Annotated Transcripts Additional Transcript Data
& Comments Reported size (kB) Comments External Data Crossreferences
Polypeptide Data Annotated
Polypeptides Additional Polypeptide
Data & Comments Reported size (kDa) Comments External Data Linkouts Crossreferences
domains - A database of protein families, domains, and functional sites
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank UniProtKB/Swiss-Prot UniProtKB/TrEMBL Mapped Features Mapped Features have been reorganized, please
see this article for details. Additional mapped features and mutations can
be found on GBrowse or related reports. External Data Linkouts Crossreferences Expression Data Transcript Expression
Additional Descriptive Data
Marker for Subcellular Localization CV Term Polypeptide Expression
Additional Descriptive Data
Marker for Subcellular Localization (GO Cellular Component) inferred from direct assay
inferred from direct assay
inferred from direct assay
inferred from mutant phenotype
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference
FlyAtlas Anatomy Microarray
Linear, scaled to maximum expression level
Tissue
Expression Level
Larval Central Nervous System
253.625
Larval Midgut
159.7
Larval Hindgut
169.2
Larval Malpighian Tubules
190.5
Larval Fat Body
94
Larval Salivary Gland
103.7
Larval Trachea
211.15
Larval Carcass
94.45
Adult Head
148.6
Adult Eye
575.075
Adult Brain
309.4
Adult Thoracic-Abdominal Ganglion
256.1
Adult Crop
138.5
Adult Midgut
190.8
Adult Hindgut
132.3
Adult Malpighian Tubules
143.4
Adult Fat Body
88.7
Adult Salivary Gland
94.7
Adult Heart
139.375
Adult VirginFemale Spermatheca
60.8
Adult InseminatedFemale Spermatheca
61.5
Adult Ovary
413.1
Adult Testis
64.6
Adult Male Accessory Gland
67.6
Adult Carcass
72.9
Expression Level Scale
None
Low
Moderate
High
Linear, scaled to Moderate expression
Tissue
Expression Level
Larval Central Nervous System
253.625
Larval Midgut
159.7
Larval Hindgut
169.2
Larval Malpighian Tubules
190.5
Larval Fat Body
94
Larval Salivary Gland
103.7
Larval Trachea
211.15
Larval Carcass
94.45
Adult Head
148.6
Adult Eye
(575.075)
Adult Brain
309.4
Adult Thoracic-Abdominal Ganglion
256.1
Adult Crop
138.5
Adult Midgut
190.8
Adult Hindgut
132.3
Adult Malpighian Tubules
143.4
Adult Fat Body
88.7
Adult Salivary Gland
94.7
Adult Heart
139.375
Adult VirginFemale Spermatheca
60.8
Adult InseminatedFemale Spermatheca
61.5
Adult Ovary
413.1
Adult Testis
64.6
Adult Male Accessory Gland
67.6
Adult Carcass
72.9
Expression Level Scale
None
Low
Moderate
High
Linear, scaled to High level expression
Tissue
Expression Level
Larval Central Nervous System
253.625
Larval Midgut
159.7
Larval Hindgut
169.2
Larval Malpighian Tubules
190.5
Larval Fat Body
94
Larval Salivary Gland
103.7
Larval Trachea
211.15
Larval Carcass
94.45
Adult Head
148.6
Adult Eye
575.075
Adult Brain
309.4
Adult Thoracic-Abdominal Ganglion
256.1
Adult Crop
138.5
Adult Midgut
190.8
Adult Hindgut
132.3
Adult Malpighian Tubules
143.4
Adult Fat Body
88.7
Adult Salivary Gland
94.7
Adult Heart
139.375
Adult VirginFemale Spermatheca
60.8
Adult InseminatedFemale Spermatheca
61.5
Adult Ovary
413.1
Adult Testis
64.6
Adult Male Accessory Gland
67.6
Adult Carcass
72.9
Expression Level Scale
None
Low
Moderate
High
Very high
Linear, scaled to Very high expression
Tissue
Expression Level
Larval Central Nervous System
253.625
Larval Midgut
159.7
Larval Hindgut
169.2
Larval Malpighian Tubules
190.5
Larval Fat Body
94
Larval Salivary Gland
103.7
Larval Trachea
211.15
Larval Carcass
94.45
Adult Head
148.6
Adult Eye
575.075
Adult Brain
309.4
Adult Thoracic-Abdominal Ganglion
256.1
Adult Crop
138.5
Adult Midgut
190.8
Adult Hindgut
132.3
Adult Malpighian Tubules
143.4
Adult Fat Body
88.7
Adult Salivary Gland
94.7
Adult Heart
139.375
Adult VirginFemale Spermatheca
60.8
Adult InseminatedFemale Spermatheca
61.5
Adult Ovary
413.1
Adult Testis
64.6
Adult Male Accessory Gland
67.6
Adult Carcass
72.9
Expression Level Scale
Very high
log, scaled to maximum expression level
Tissue
Expression Level
Larval Central Nervous System
253.625
Larval Midgut
159.7
Larval Hindgut
169.2
Larval Malpighian Tubules
190.5
Larval Fat Body
94
Larval Salivary Gland
103.7
Larval Trachea
211.15
Larval Carcass
94.45
Adult Head
148.6
Adult Eye
575.075
Adult Brain
309.4
Adult Thoracic-Abdominal Ganglion
256.1
Adult Crop
138.5
Adult Midgut
190.8
Adult Hindgut
132.3
Adult Malpighian Tubules
143.4
Adult Fat Body
88.7
Adult Salivary Gland
94.7
Adult Heart
139.375
Adult VirginFemale Spermatheca
60.8
Adult InseminatedFemale Spermatheca
61.5
Adult Ovary
413.1
Adult Testis
64.6
Adult Male Accessory Gland
67.6
Adult Carcass
72.9
Expression Level Scale
None
Low
Moderate
High
Very high
log, scaled to Moderate expression
Tissue
Expression Level
Larval Central Nervous System
253.625
Larval Midgut
159.7
Larval Hindgut
169.2
Larval Malpighian Tubules
190.5
Larval Fat Body
94
Larval Salivary Gland
103.7
Larval Trachea
211.15
Larval Carcass
94.45
Adult Head
148.6
Adult Eye
575.075
Adult Brain
309.4
Adult Thoracic-Abdominal Ganglion
256.1
Adult Crop
138.5
Adult Midgut
190.8
Adult Hindgut
132.3
Adult Malpighian Tubules
143.4
Adult Fat Body
88.7
Adult Salivary Gland
94.7
Adult Heart
139.375
Adult VirginFemale Spermatheca
60.8
Adult InseminatedFemale Spermatheca
61.5
Adult Ovary
413.1
Adult Testis
64.6
Adult Male Accessory Gland
67.6
Adult Carcass
72.9
Expression Level Scale
None
Low
Moderate
High
log, scaled to High level expression
Tissue
Expression Level
Larval Central Nervous System
253.625
Larval Midgut
159.7
Larval Hindgut
169.2
Larval Malpighian Tubules
190.5
Larval Fat Body
94
Larval Salivary Gland
103.7
Larval Trachea
211.15
Larval Carcass
94.45
Adult Head
148.6
Adult Eye
575.075
Adult Brain
309.4
Adult Thoracic-Abdominal Ganglion
256.1
Adult Crop
138.5
Adult Midgut
190.8
Adult Hindgut
132.3
Adult Malpighian Tubules
143.4
Adult Fat Body
88.7
Adult Salivary Gland
94.7
Adult Heart
139.375
Adult VirginFemale Spermatheca
60.8
Adult InseminatedFemale Spermatheca
61.5
Adult Ovary
413.1
Adult Testis
64.6
Adult Male Accessory Gland
67.6
Adult Carcass
72.9
Expression Level Scale
None
Low
Moderate
High
Very high
log, scaled to Very high expression
Tissue
Expression Level
Larval Central Nervous System
253.625
Larval Midgut
159.7
Larval Hindgut
169.2
Larval Malpighian Tubules
190.5
Larval Fat Body
94
Larval Salivary Gland
103.7
Larval Trachea
211.15
Larval Carcass
94.45
Adult Head
148.6
Adult Eye
575.075
Adult Brain
309.4
Adult Thoracic-Abdominal Ganglion
256.1
Adult Crop
138.5
Adult Midgut
190.8
Adult Hindgut
132.3
Adult Malpighian Tubules
143.4
Adult Fat Body
88.7
Adult Salivary Gland
94.7
Adult Heart
139.375
Adult VirginFemale Spermatheca
60.8
Adult InseminatedFemale Spermatheca
61.5
Adult Ovary
413.1
Adult Testis
64.6
Adult Male Accessory Gland
67.6
Adult Carcass
72.9
Expression Level Scale
None
Low
Moderate
High
Very high
Heatmap
Tissue
Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass
FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level
Tissue
Adult Expression Level
NA
Head
148.6
NA
Eye
575.075
NA
Brain
309.4
253.625
Central Nervous System
NA
NA
Thoracic-Abdominal Ganglion
256.1
NA
Crop
138.5
159.7
Midgut
190.8
169.2
Hindgut
132.3
190.5
Malpighian Tubules
143.4
94
Fat Body
88.7
103.7
Salivary Gland
94.7
NA
Heart
139.375
211.15
Trachea
NA
NA
VirginFemale Spermatheca
60.8
NA
InseminatedFemale Spermatheca
61.5
NA
Ovary
413.1
NA
Testis
64.6
NA
Male Accessory Gland
67.6
94.45
Carcass
72.9
modENCODE Anatomy RNA-Seq
Linear, scaled to maximum expression level
Tissue
Expression Level
imaginal disc, larvae L3 wandering
58
central nervous system, larvae L3
39
central nervous system, pupae P8
48
head, virgin 1-day female
26
head, virgin 4-day female
25
head, virgin 20-day female
13
head, mated 1-day female
28
head, mated 4-day female
21
head, mated 20-day female
15
head, mated 1-day male
34
head, mated 4-day male
37
head, mated 20-day male
26
salivary gland, larvae L3 wandering
28
salivary gland, white prepupae
157
digestive system, larvae L3 wandering
15
digestive system, 1-day adult
12
digestive system, 4-day adult
22
digestive system, 20-day adult
14
fat body, larvae L3 wandering
8
fat body, white prepupae
25
fat body, pupae P8
36
carcass, larvae L3 wandering
22
carcass, 1-day adult
11
carcass, 4-day adult
15
carcass, 20-day adult
12
ovary, virgin 4-day female
58
ovary, mated 4-day female
63
testis, mated 4-day male
57
accessory gland, mated 4-day male
12
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Linear, scaled to Moderate expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
(58)
central nervous system, larvae L3
(39)
central nervous system, pupae P8
(48)
head, virgin 1-day female
26
head, virgin 4-day female
25
head, virgin 20-day female
13
head, mated 1-day female
(28)
head, mated 4-day female
21
head, mated 20-day female
15
head, mated 1-day male
(34)
head, mated 4-day male
(37)
head, mated 20-day male
26
salivary gland, larvae L3 wandering
(28)
salivary gland, white prepupae
(157)
digestive system, larvae L3 wandering
15
digestive system, 1-day adult
12
digestive system, 4-day adult
22
digestive system, 20-day adult
14
fat body, larvae L3 wandering
8
fat body, white prepupae
25
fat body, pupae P8
(36)
carcass, larvae L3 wandering
22
carcass, 1-day adult
11
carcass, 4-day adult
15
carcass, 20-day adult
12
ovary, virgin 4-day female
(58)
ovary, mated 4-day female
(63)
testis, mated 4-day male
(57)
accessory gland, mated 4-day male
12
Expression Level Scale
Very low
Low
Moderate
Moderately high
Linear, scaled to High expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
58
central nervous system, larvae L3
39
central nervous system, pupae P8
48
head, virgin 1-day female
26
head, virgin 4-day female
25
head, virgin 20-day female
13
head, mated 1-day female
28
head, mated 4-day female
21
head, mated 20-day female
15
head, mated 1-day male
34
head, mated 4-day male
37
head, mated 20-day male
26
salivary gland, larvae L3 wandering
28
salivary gland, white prepupae
(157)
digestive system, larvae L3 wandering
15
digestive system, 1-day adult
12
digestive system, 4-day adult
22
digestive system, 20-day adult
14
fat body, larvae L3 wandering
8
fat body, white prepupae
25
fat body, pupae P8
36
carcass, larvae L3 wandering
22
carcass, 1-day adult
11
carcass, 4-day adult
15
carcass, 20-day adult
12
ovary, virgin 4-day female
58
ovary, mated 4-day female
63
testis, mated 4-day male
57
accessory gland, mated 4-day male
12
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Linear, scaled to Extremely high expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
58
central nervous system, larvae L3
39
central nervous system, pupae P8
48
head, virgin 1-day female
26
head, virgin 4-day female
25
head, virgin 20-day female
13
head, mated 1-day female
28
head, mated 4-day female
21
head, mated 20-day female
15
head, mated 1-day male
34
head, mated 4-day male
37
head, mated 20-day male
26
salivary gland, larvae L3 wandering
28
salivary gland, white prepupae
157
digestive system, larvae L3 wandering
15
digestive system, 1-day adult
12
digestive system, 4-day adult
22
digestive system, 20-day adult
14
fat body, larvae L3 wandering
8
fat body, white prepupae
25
fat body, pupae P8
36
carcass, larvae L3 wandering
22
carcass, 1-day adult
11
carcass, 4-day adult
15
carcass, 20-day adult
12
ovary, virgin 4-day female
58
ovary, mated 4-day female
63
testis, mated 4-day male
57
accessory gland, mated 4-day male
12
Expression Level Scale
Extremely high
log, scaled to maximum expression level
Tissue
Expression Level
imaginal disc, larvae L3 wandering
58
central nervous system, larvae L3
39
central nervous system, pupae P8
48
head, virgin 1-day female
26
head, virgin 4-day female
25
head, virgin 20-day female
13
head, mated 1-day female
28
head, mated 4-day female
21
head, mated 20-day female
15
head, mated 1-day male
34
head, mated 4-day male
37
head, mated 20-day male
26
salivary gland, larvae L3 wandering
28
salivary gland, white prepupae
157
digestive system, larvae L3 wandering
15
digestive system, 1-day adult
12
digestive system, 4-day adult
22
digestive system, 20-day adult
14
fat body, larvae L3 wandering
8
fat body, white prepupae
25
fat body, pupae P8
36
carcass, larvae L3 wandering
22
carcass, 1-day adult
11
carcass, 4-day adult
15
carcass, 20-day adult
12
ovary, virgin 4-day female
58
ovary, mated 4-day female
63
testis, mated 4-day male
57
accessory gland, mated 4-day male
12
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
log, scaled to Moderate expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
(58)
central nervous system, larvae L3
(39)
central nervous system, pupae P8
(48)
head, virgin 1-day female
26
head, virgin 4-day female
25
head, virgin 20-day female
13
head, mated 1-day female
28
head, mated 4-day female
21
head, mated 20-day female
15
head, mated 1-day male
(34)
head, mated 4-day male
(37)
head, mated 20-day male
26
salivary gland, larvae L3 wandering
28
salivary gland, white prepupae
(157)
digestive system, larvae L3 wandering
15
digestive system, 1-day adult
12
digestive system, 4-day adult
22
digestive system, 20-day adult
14
fat body, larvae L3 wandering
8
fat body, white prepupae
25
fat body, pupae P8
(36)
carcass, larvae L3 wandering
22
carcass, 1-day adult
11
carcass, 4-day adult
15
carcass, 20-day adult
12
ovary, virgin 4-day female
(58)
ovary, mated 4-day female
(63)
testis, mated 4-day male
(57)
accessory gland, mated 4-day male
12
Expression Level Scale
Very low
Low
Moderate
Moderately high
log, scaled to High expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
58
central nervous system, larvae L3
39
central nervous system, pupae P8
48
head, virgin 1-day female
26
head, virgin 4-day female
25
head, virgin 20-day female
13
head, mated 1-day female
28
head, mated 4-day female
21
head, mated 20-day female
15
head, mated 1-day male
34
head, mated 4-day male
37
head, mated 20-day male
26
salivary gland, larvae L3 wandering
28
salivary gland, white prepupae
(157)
digestive system, larvae L3 wandering
15
digestive system, 1-day adult
12
digestive system, 4-day adult
22
digestive system, 20-day adult
14
fat body, larvae L3 wandering
8
fat body, white prepupae
25
fat body, pupae P8
36
carcass, larvae L3 wandering
22
carcass, 1-day adult
11
carcass, 4-day adult
15
carcass, 20-day adult
12
ovary, virgin 4-day female
58
ovary, mated 4-day female
63
testis, mated 4-day male
57
accessory gland, mated 4-day male
12
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
log, scaled to Extremely high expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
58
central nervous system, larvae L3
39
central nervous system, pupae P8
48
head, virgin 1-day female
26
head, virgin 4-day female
25
head, virgin 20-day female
13
head, mated 1-day female
28
head, mated 4-day female
21
head, mated 20-day female
15
head, mated 1-day male
34
head, mated 4-day male
37
head, mated 20-day male
26
salivary gland, larvae L3 wandering
28
salivary gland, white prepupae
157
digestive system, larvae L3 wandering
15
digestive system, 1-day adult
12
digestive system, 4-day adult
22
digestive system, 20-day adult
14
fat body, larvae L3 wandering
8
fat body, white prepupae
25
fat body, pupae P8
36
carcass, larvae L3 wandering
22
carcass, 1-day adult
11
carcass, 4-day adult
15
carcass, 20-day adult
12
ovary, virgin 4-day female
58
ovary, mated 4-day female
63
testis, mated 4-day male
57
accessory gland, mated 4-day male
12
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Extremely high
Heatmap
Tissue
Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male
modENCODE Development RNA-Seq
Linear, scaled to maximum expression level
Developmental Stage
Expression Level
embryo 00-02hr
34
embryo 02-04hr
39
embryo 04-06hr
34
embryo 06-08hr
32
embryo 08-10hr
38
embryo 10-12hr
22
embryo 12-14hr
31
embryo 14-16hr
17
embryo 16-18hr
20
embryo 18-20hr
12
embryo 20-22hr
26
embryo 22-24hr
27
larva L1
9
larva L2
10
larva L3 12hr old
7
larva L3 puffstage 1-2
7
larva L3 puffstage 3-6
14
larva L3 puffstage 7-9
24
white prepupae new
28
white prepupae 12hr
33
white prepupae 24hr
35
pupae 2d postWPP
32
pupae 3d postWPP
25
pupae 4d postWPP
25
adult male 01day
30
adult male 05day
33
adult male 30day
26
adult female 01day
14
adult female 05day
28
adult female 30day
24
Expression Level Scale
Very low
Low
Moderate
Moderately high
Linear, scaled to Moderate expression
Developmental Stage
Expression Level
embryo 00-02hr
(34)
embryo 02-04hr
(39)
embryo 04-06hr
(34)
embryo 06-08hr
(32)
embryo 08-10hr
(38)
embryo 10-12hr
22
embryo 12-14hr
(31)
embryo 14-16hr
17
embryo 16-18hr
20
embryo 18-20hr
12
embryo 20-22hr
26
embryo 22-24hr
(27)
larva L1
9
larva L2
10
larva L3 12hr old
7
larva L3 puffstage 1-2
7
larva L3 puffstage 3-6
14
larva L3 puffstage 7-9
24
white prepupae new
(28)
white prepupae 12hr
(33)
white prepupae 24hr
(35)
pupae 2d postWPP
(32)
pupae 3d postWPP
25
pupae 4d postWPP
25
adult male 01day
(30)
adult male 05day
(33)
adult male 30day
26
adult female 01day
14
adult female 05day
(28)
adult female 30day
24
Expression Level Scale
Very low
Low
Moderate
Moderately high
Linear, scaled to High expression
Developmental Stage
Expression Level
embryo 00-02hr
34
embryo 02-04hr
39
embryo 04-06hr
34
embryo 06-08hr
32
embryo 08-10hr
38
embryo 10-12hr
22
embryo 12-14hr
31
embryo 14-16hr
17
embryo 16-18hr
20
embryo 18-20hr
12
embryo 20-22hr
26
embryo 22-24hr
27
larva L1
9
larva L2
10
larva L3 12hr old
7
larva L3 puffstage 1-2
7
larva L3 puffstage 3-6
14
larva L3 puffstage 7-9
24
white prepupae new
28
white prepupae 12hr
33
white prepupae 24hr
35
pupae 2d postWPP
32
pupae 3d postWPP
25
pupae 4d postWPP
25
adult male 01day
30
adult male 05day
33
adult male 30day
26
adult female 01day
14
adult female 05day
28
adult female 30day
24
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Linear, scaled to Extremely high expression
Developmental Stage
Expression Level
embryo 00-02hr
34
embryo 02-04hr
39
embryo 04-06hr
34
embryo 06-08hr
32
embryo 08-10hr
38
embryo 10-12hr
22
embryo 12-14hr
31
embryo 14-16hr
17
embryo 16-18hr
20
embryo 18-20hr
12
embryo 20-22hr
26
embryo 22-24hr
27
larva L1
9
larva L2
10
larva L3 12hr old
7
larva L3 puffstage 1-2
7
larva L3 puffstage 3-6
14
larva L3 puffstage 7-9
24
white prepupae new
28
white prepupae 12hr
33
white prepupae 24hr
35
pupae 2d postWPP
32
pupae 3d postWPP
25
pupae 4d postWPP
25
adult male 01day
30
adult male 05day
33
adult male 30day
26
adult female 01day
14
adult female 05day
28
adult female 30day
24
Expression Level Scale
Extremely high
log, scaled to maximum expression level
Developmental Stage
Expression Level
embryo 00-02hr
34
embryo 02-04hr
39
embryo 04-06hr
34
embryo 06-08hr
32
embryo 08-10hr
38
embryo 10-12hr
22
embryo 12-14hr
31
embryo 14-16hr
17
embryo 16-18hr
20
embryo 18-20hr
12
embryo 20-22hr
26
embryo 22-24hr
27
larva L1
9
larva L2
10
larva L3 12hr old
7
larva L3 puffstage 1-2
7
larva L3 puffstage 3-6
14
larva L3 puffstage 7-9
24
white prepupae new
28
white prepupae 12hr
33
white prepupae 24hr
35
pupae 2d postWPP
32
pupae 3d postWPP
25
pupae 4d postWPP
25
adult male 01day
30
adult male 05day
33
adult male 30day
26
adult female 01day
14
adult female 05day
28
adult female 30day
24
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
log, scaled to Moderate expression
Developmental Stage
Expression Level
embryo 00-02hr
(34)
embryo 02-04hr
(39)
embryo 04-06hr
(34)
embryo 06-08hr
32
embryo 08-10hr
(38)
embryo 10-12hr
22
embryo 12-14hr
31
embryo 14-16hr
17
embryo 16-18hr
20
embryo 18-20hr
12
embryo 20-22hr
26
embryo 22-24hr
27
larva L1
9
larva L2
10
larva L3 12hr old
7
larva L3 puffstage 1-2
7
larva L3 puffstage 3-6
14
larva L3 puffstage 7-9
24
white prepupae new
28
white prepupae 12hr
(33)
white prepupae 24hr
(35)
pupae 2d postWPP
32
pupae 3d postWPP
25
pupae 4d postWPP
25
adult male 01day
30
adult male 05day
(33)
adult male 30day
26
adult female 01day
14
adult female 05day
28
adult female 30day
24
Expression Level Scale
Very low
Low
Moderate
Moderately high
log, scaled to High expression
Developmental Stage
Expression Level
embryo 00-02hr
34
embryo 02-04hr
39
embryo 04-06hr
34
embryo 06-08hr
32
embryo 08-10hr
38
embryo 10-12hr
22
embryo 12-14hr
31
embryo 14-16hr
17
embryo 16-18hr
20
embryo 18-20hr
12
embryo 20-22hr
26
embryo 22-24hr
27
larva L1
9
larva L2
10
larva L3 12hr old
7
larva L3 puffstage 1-2
7
larva L3 puffstage 3-6
14
larva L3 puffstage 7-9
24
white prepupae new
28
white prepupae 12hr
33
white prepupae 24hr
35
pupae 2d postWPP
32
pupae 3d postWPP
25
pupae 4d postWPP
25
adult male 01day
30
adult male 05day
33
adult male 30day
26
adult female 01day
14
adult female 05day
28
adult female 30day
24
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
log, scaled to Extremely high expression
Developmental Stage
Expression Level
embryo 00-02hr
34
embryo 02-04hr
39
embryo 04-06hr
34
embryo 06-08hr
32
embryo 08-10hr
38
embryo 10-12hr
22
embryo 12-14hr
31
embryo 14-16hr
17
embryo 16-18hr
20
embryo 18-20hr
12
embryo 20-22hr
26
embryo 22-24hr
27
larva L1
9
larva L2
10
larva L3 12hr old
7
larva L3 puffstage 1-2
7
larva L3 puffstage 3-6
14
larva L3 puffstage 7-9
24
white prepupae new
28
white prepupae 12hr
33
white prepupae 24hr
35
pupae 2d postWPP
32
pupae 3d postWPP
25
pupae 4d postWPP
25
adult male 01day
30
adult male 05day
33
adult male 30day
26
adult female 01day
14
adult female 05day
28
adult female 30day
24
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Extremely high
Heatmap
Developmental Stage
Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day
modENCODE Cell Lines RNA-Seq
Linear, scaled to maximum expression level
Cell Line
Expression Level
Schneider line 2 S2R+
38
Schneider line 2 Sg4
15
embryonic 1182-4H
17
embryonic GM2
33
embryonic Kc167
28
embryonic S1
24
embryonic S3
31
leg disc CME L1
19
wing disc CME-W2
27
wing disc ML-DmD8
30
wing disc ML-DmD9
17
wing disc ML-DmD16-c3
26
wing disc ML-DmD21
7
wing disc ML-DmD32
19
haltere disc ML-DmD17-c3
34
eye-antennal disc ML-DmD11
18
antennal disc ML-DmD20-c5
15
mixed discs ML-DmD4-c1
21
CNS ML-DmBG1-c1
23
CNS ML-DmBG2-c2
17
tumorous blood cells mbn2
42
ovary fGS/OSS
23
ovary OSC
36
ovary OSS
26
Expression Level Scale
Very low
Low
Moderate
Moderately high
Linear, scaled to Moderate expression
Cell Line
Expression Level
Schneider line 2 S2R+
(38)
Schneider line 2 Sg4
15
embryonic 1182-4H
17
embryonic GM2
(33)
embryonic Kc167
(28)
embryonic S1
24
embryonic S3
(31)
leg disc CME L1
19
wing disc CME-W2
(27)
wing disc ML-DmD8
(30)
wing disc ML-DmD9
17
wing disc ML-DmD16-c3
26
wing disc ML-DmD21
7
wing disc ML-DmD32
19
haltere disc ML-DmD17-c3
(34)
eye-antennal disc ML-DmD11
18
antennal disc ML-DmD20-c5
15
mixed discs ML-DmD4-c1
21
CNS ML-DmBG1-c1
23
CNS ML-DmBG2-c2
17
tumorous blood cells mbn2
(42)
ovary fGS/OSS
23
ovary OSC
(36)
ovary OSS
26
Expression Level Scale
Very low
Low
Moderate
Moderately high
Linear, scaled to High expression
Cell Line
Expression Level
Schneider line 2 S2R+
38
Schneider line 2 Sg4
15
embryonic 1182-4H
17
embryonic GM2
33
embryonic Kc167
28
embryonic S1
24
embryonic S3
31
leg disc CME L1
19
wing disc CME-W2
27
wing disc ML-DmD8
30
wing disc ML-DmD9
17
wing disc ML-DmD16-c3
26
wing disc ML-DmD21
7
wing disc ML-DmD32
19
haltere disc ML-DmD17-c3
34
eye-antennal disc ML-DmD11
18
antennal disc ML-DmD20-c5
15
mixed discs ML-DmD4-c1
21
CNS ML-DmBG1-c1
23
CNS ML-DmBG2-c2
17
tumorous blood cells mbn2
42
ovary fGS/OSS
23
ovary OSC
36
ovary OSS
26
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Linear, scaled to Extremely high expression
Cell Line
Expression Level
Schneider line 2 S2R+
38
Schneider line 2 Sg4
15
embryonic 1182-4H
17
embryonic GM2
33
embryonic Kc167
28
embryonic S1
24
embryonic S3
31
leg disc CME L1
19
wing disc CME-W2
27
wing disc ML-DmD8
30
wing disc ML-DmD9
17
wing disc ML-DmD16-c3
26
wing disc ML-DmD21
7
wing disc ML-DmD32
19
haltere disc ML-DmD17-c3
34
eye-antennal disc ML-DmD11
18
antennal disc ML-DmD20-c5
15
mixed discs ML-DmD4-c1
21
CNS ML-DmBG1-c1
23
CNS ML-DmBG2-c2
17
tumorous blood cells mbn2
42
ovary fGS/OSS
23
ovary OSC
36
ovary OSS
26
Expression Level Scale
Extremely high
log, scaled to maximum expression level
Cell Line
Expression Level
Schneider line 2 S2R+
38
Schneider line 2 Sg4
15
embryonic 1182-4H
17
embryonic GM2
33
embryonic Kc167
28
embryonic S1
24
embryonic S3
31
leg disc CME L1
19
wing disc CME-W2
27
wing disc ML-DmD8
30
wing disc ML-DmD9
17
wing disc ML-DmD16-c3
26
wing disc ML-DmD21
7
wing disc ML-DmD32
19
haltere disc ML-DmD17-c3
34
eye-antennal disc ML-DmD11
18
antennal disc ML-DmD20-c5
15
mixed discs ML-DmD4-c1
21
CNS ML-DmBG1-c1
23
CNS ML-DmBG2-c2
17
tumorous blood cells mbn2
42
ovary fGS/OSS
23
ovary OSC
36
ovary OSS
26
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
log, scaled to Moderate expression
Cell Line
Expression Level
Schneider line 2 S2R+
(38)
Schneider line 2 Sg4
15
embryonic 1182-4H
17
embryonic GM2
(33)
embryonic Kc167
28
embryonic S1
24
embryonic S3
31
leg disc CME L1
19
wing disc CME-W2
27
wing disc ML-DmD8
30
wing disc ML-DmD9
17
wing disc ML-DmD16-c3
26
wing disc ML-DmD21
7
wing disc ML-DmD32
19
haltere disc ML-DmD17-c3
(34)
eye-antennal disc ML-DmD11
18
antennal disc ML-DmD20-c5
15
mixed discs ML-DmD4-c1
21
CNS ML-DmBG1-c1
23
CNS ML-DmBG2-c2
17
tumorous blood cells mbn2
(42)
ovary fGS/OSS
23
ovary OSC
(36)
ovary OSS
26
Expression Level Scale
Very low
Low
Moderate
Moderately high
log, scaled to High expression
Cell Line
Expression Level
Schneider line 2 S2R+
38
Schneider line 2 Sg4
15
embryonic 1182-4H
17
embryonic GM2
33
embryonic Kc167
28
embryonic S1
24
embryonic S3
31
leg disc CME L1
19
wing disc CME-W2
27
wing disc ML-DmD8
30
wing disc ML-DmD9
17
wing disc ML-DmD16-c3
26
wing disc ML-DmD21
7
wing disc ML-DmD32
19
haltere disc ML-DmD17-c3
34
eye-antennal disc ML-DmD11
18
antennal disc ML-DmD20-c5
15
mixed discs ML-DmD4-c1
21
CNS ML-DmBG1-c1
23
CNS ML-DmBG2-c2
17
tumorous blood cells mbn2
42
ovary fGS/OSS
23
ovary OSC
36
ovary OSS
26
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
log, scaled to Extremely high expression
Cell Line
Expression Level
Schneider line 2 S2R+
38
Schneider line 2 Sg4
15
embryonic 1182-4H
17
embryonic GM2
33
embryonic Kc167
28
embryonic S1
24
embryonic S3
31
leg disc CME L1
19
wing disc CME-W2
27
wing disc ML-DmD8
30
wing disc ML-DmD9
17
wing disc ML-DmD16-c3
26
wing disc ML-DmD21
7
wing disc ML-DmD32
19
haltere disc ML-DmD17-c3
34
eye-antennal disc ML-DmD11
18
antennal disc ML-DmD20-c5
15
mixed discs ML-DmD4-c1
21
CNS ML-DmBG1-c1
23
CNS ML-DmBG2-c2
17
tumorous blood cells mbn2
42
ovary fGS/OSS
23
ovary OSC
36
ovary OSS
26
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Extremely high
Heatmap
Cell Line
Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS
modENCODE Treatments RNA-Seq
Linear, scaled to maximum expression level
Treatment
Expression Level
extended cold, 4-day adult
13
cold shock, 4-day adult
10
heat shock, 4-day adult
19
Cadmium 50 mM 6 hrs, larvae L3
6
Cadmium 50 mM 12 hrs, larvae L3
19
Cadmium 50 mM 48 hrs, 4-day adult
24
Cadmium 100 mM 48 hrs, 4-day adult
61
Copper 0.5 mM 12 hrs, larvae L3
5
Copper 15 mM 48 hrs, 4-day adult
145
Zinc 5 mM 12 hrs, larvae L3
7
Zinc 4.5 mM 48 hrs, 4-day adult
59
Ethanol 2.5% 3 hrs, larvae L3
29
Ethanol 5% 3 hrs, larvae L3
11
Ethanol 10% 3 hrs, larvae L3
8
Caffeine 1.5 mg/ml 4 hrs, larvae L3
10
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
51
Caffeine 25 mg/ml 48 hrs, 4-day adult
25
Paraquat 5 mM 48 hrs, 4-day adult
44
Paraquat 10 mM 48 hrs, 4-day adult
29
Rotenone 2 μg 12 hrs, larvae L3
11
Rotenone 8 μg 12 hrs, larvae L3
5
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Linear, scaled to Moderate expression
Treatment
Expression Level
extended cold, 4-day adult
13
cold shock, 4-day adult
10
heat shock, 4-day adult
19
Cadmium 50 mM 6 hrs, larvae L3
6
Cadmium 50 mM 12 hrs, larvae L3
19
Cadmium 50 mM 48 hrs, 4-day adult
24
Cadmium 100 mM 48 hrs, 4-day adult
(61)
Copper 0.5 mM 12 hrs, larvae L3
5
Copper 15 mM 48 hrs, 4-day adult
(145)
Zinc 5 mM 12 hrs, larvae L3
7
Zinc 4.5 mM 48 hrs, 4-day adult
(59)
Ethanol 2.5% 3 hrs, larvae L3
(29)
Ethanol 5% 3 hrs, larvae L3
11
Ethanol 10% 3 hrs, larvae L3
8
Caffeine 1.5 mg/ml 4 hrs, larvae L3
10
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
(51)
Caffeine 25 mg/ml 48 hrs, 4-day adult
25
Paraquat 5 mM 48 hrs, 4-day adult
(44)
Paraquat 10 mM 48 hrs, 4-day adult
(29)
Rotenone 2 μg 12 hrs, larvae L3
11
Rotenone 8 μg 12 hrs, larvae L3
5
Expression Level Scale
Very low
Low
Moderate
Moderately high
Linear, scaled to High expression
Treatment
Expression Level
extended cold, 4-day adult
13
cold shock, 4-day adult
10
heat shock, 4-day adult
19
Cadmium 50 mM 6 hrs, larvae L3
6
Cadmium 50 mM 12 hrs, larvae L3
19
Cadmium 50 mM 48 hrs, 4-day adult
24
Cadmium 100 mM 48 hrs, 4-day adult
61
Copper 0.5 mM 12 hrs, larvae L3
5
Copper 15 mM 48 hrs, 4-day adult
(145)
Zinc 5 mM 12 hrs, larvae L3
7
Zinc 4.5 mM 48 hrs, 4-day adult
59
Ethanol 2.5% 3 hrs, larvae L3
29
Ethanol 5% 3 hrs, larvae L3
11
Ethanol 10% 3 hrs, larvae L3
8
Caffeine 1.5 mg/ml 4 hrs, larvae L3
10
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
51
Caffeine 25 mg/ml 48 hrs, 4-day adult
25
Paraquat 5 mM 48 hrs, 4-day adult
44
Paraquat 10 mM 48 hrs, 4-day adult
29
Rotenone 2 μg 12 hrs, larvae L3
11
Rotenone 8 μg 12 hrs, larvae L3
5
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Linear, scaled to Extremely high expression
Treatment
Expression Level
extended cold, 4-day adult
13
cold shock, 4-day adult
10
heat shock, 4-day adult
19
Cadmium 50 mM 6 hrs, larvae L3
6
Cadmium 50 mM 12 hrs, larvae L3
19
Cadmium 50 mM 48 hrs, 4-day adult
24
Cadmium 100 mM 48 hrs, 4-day adult
61
Copper 0.5 mM 12 hrs, larvae L3
5
Copper 15 mM 48 hrs, 4-day adult
145
Zinc 5 mM 12 hrs, larvae L3
7
Zinc 4.5 mM 48 hrs, 4-day adult
59
Ethanol 2.5% 3 hrs, larvae L3
29
Ethanol 5% 3 hrs, larvae L3
11
Ethanol 10% 3 hrs, larvae L3
8
Caffeine 1.5 mg/ml 4 hrs, larvae L3
10
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
51
Caffeine 25 mg/ml 48 hrs, 4-day adult
25
Paraquat 5 mM 48 hrs, 4-day adult
44
Paraquat 10 mM 48 hrs, 4-day adult
29
Rotenone 2 μg 12 hrs, larvae L3
11
Rotenone 8 μg 12 hrs, larvae L3
5
Expression Level Scale
Extremely high
log, scaled to maximum expression level
Treatment
Expression Level
extended cold, 4-day adult
13
cold shock, 4-day adult
10
heat shock, 4-day adult
19
Cadmium 50 mM 6 hrs, larvae L3
6
Cadmium 50 mM 12 hrs, larvae L3
19
Cadmium 50 mM 48 hrs, 4-day adult
24
Cadmium 100 mM 48 hrs, 4-day adult
61
Copper 0.5 mM 12 hrs, larvae L3
5
Copper 15 mM 48 hrs, 4-day adult
145
Zinc 5 mM 12 hrs, larvae L3
7
Zinc 4.5 mM 48 hrs, 4-day adult
59
Ethanol 2.5% 3 hrs, larvae L3
29
Ethanol 5% 3 hrs, larvae L3
11
Ethanol 10% 3 hrs, larvae L3
8
Caffeine 1.5 mg/ml 4 hrs, larvae L3
10
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
51
Caffeine 25 mg/ml 48 hrs, 4-day adult
25
Paraquat 5 mM 48 hrs, 4-day adult
44
Paraquat 10 mM 48 hrs, 4-day adult
29
Rotenone 2 μg 12 hrs, larvae L3
11
Rotenone 8 μg 12 hrs, larvae L3
5
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
log, scaled to Moderate expression
Treatment
Expression Level
extended cold, 4-day adult
13
cold shock, 4-day adult
10
heat shock, 4-day adult
19
Cadmium 50 mM 6 hrs, larvae L3
6
Cadmium 50 mM 12 hrs, larvae L3
19
Cadmium 50 mM 48 hrs, 4-day adult
24
Cadmium 100 mM 48 hrs, 4-day adult
(61)
Copper 0.5 mM 12 hrs, larvae L3
5
Copper 15 mM 48 hrs, 4-day adult
(145)
Zinc 5 mM 12 hrs, larvae L3
7
Zinc 4.5 mM 48 hrs, 4-day adult
(59)
Ethanol 2.5% 3 hrs, larvae L3
29
Ethanol 5% 3 hrs, larvae L3
11
Ethanol 10% 3 hrs, larvae L3
8
Caffeine 1.5 mg/ml 4 hrs, larvae L3
10
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
(51)
Caffeine 25 mg/ml 48 hrs, 4-day adult
25
Paraquat 5 mM 48 hrs, 4-day adult
(44)
Paraquat 10 mM 48 hrs, 4-day adult
29
Rotenone 2 μg 12 hrs, larvae L3
11
Rotenone 8 μg 12 hrs, larvae L3
5
Expression Level Scale
Very low
Low
Moderate
Moderately high
log, scaled to High expression
Treatment
Expression Level
extended cold, 4-day adult
13
cold shock, 4-day adult
10
heat shock, 4-day adult
19
Cadmium 50 mM 6 hrs, larvae L3
6
Cadmium 50 mM 12 hrs, larvae L3
19
Cadmium 50 mM 48 hrs, 4-day adult
24
Cadmium 100 mM 48 hrs, 4-day adult
61
Copper 0.5 mM 12 hrs, larvae L3
5
Copper 15 mM 48 hrs, 4-day adult
(145)
Zinc 5 mM 12 hrs, larvae L3
7
Zinc 4.5 mM 48 hrs, 4-day adult
59
Ethanol 2.5% 3 hrs, larvae L3
29
Ethanol 5% 3 hrs, larvae L3
11
Ethanol 10% 3 hrs, larvae L3
8
Caffeine 1.5 mg/ml 4 hrs, larvae L3
10
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
51
Caffeine 25 mg/ml 48 hrs, 4-day adult
25
Paraquat 5 mM 48 hrs, 4-day adult
44
Paraquat 10 mM 48 hrs, 4-day adult
29
Rotenone 2 μg 12 hrs, larvae L3
11
Rotenone 8 μg 12 hrs, larvae L3
5
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
log, scaled to Extremely high expression
Treatment
Expression Level
extended cold, 4-day adult
13
cold shock, 4-day adult
10
heat shock, 4-day adult
19
Cadmium 50 mM 6 hrs, larvae L3
6
Cadmium 50 mM 12 hrs, larvae L3
19
Cadmium 50 mM 48 hrs, 4-day adult
24
Cadmium 100 mM 48 hrs, 4-day adult
61
Copper 0.5 mM 12 hrs, larvae L3
5
Copper 15 mM 48 hrs, 4-day adult
145
Zinc 5 mM 12 hrs, larvae L3
7
Zinc 4.5 mM 48 hrs, 4-day adult
59
Ethanol 2.5% 3 hrs, larvae L3
29
Ethanol 5% 3 hrs, larvae L3
11
Ethanol 10% 3 hrs, larvae L3
8
Caffeine 1.5 mg/ml 4 hrs, larvae L3
10
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
51
Caffeine 25 mg/ml 48 hrs, 4-day adult
25
Paraquat 5 mM 48 hrs, 4-day adult
44
Paraquat 10 mM 48 hrs, 4-day adult
29
Rotenone 2 μg 12 hrs, larvae L3
11
Rotenone 8 μg 12 hrs, larvae L3
5
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Extremely high
Heatmap
Treatment
Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
A cluster of genes with similar mRNA expression dynamics across development.
External Data & Images
Linkouts
- Cell culture data for RNAi and other high-throughput technologies
Alleles & Phenotypes Summary of Allele Phenotypes Classical Alleles ( 48 ) For All Classical Alleles Show Alleles Carried on Transgenic Constructs
( 15 ) For All Alleles Carried on Transgenic
Constructs Show Aneuploid Aberrations Disrupted in Not disrupted in Transgenic Constructs & Insertions
Transgenic Constructs UAS construct
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
Insertions insertion of enhancer trap
insertion of mobile activating element
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
miscellaneous insertions
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
insertion of enhancer trap binary system
Gene Ontology: Function, Process
& Cellular Component ( 16 unique terms ) Terms Based on
Experimental Evidence ( 14 terms ) Molecular Function ( 0 terms) Biological Process inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with
nmo inferred from mutant phenotype
inferred from direct assay
inferred from mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with
mwh inferred from mutant phenotype
inferred from mutant phenotype
inferred from direct assay
inferred from mutant phenotype
Cellular Component inferred from direct assay
inferred from direct assay
inferred from direct assay
inferred from mutant phenotype
Terms Based on Predictions or
Assertions ( 3 terms ) Molecular Function inferred from sequence or structural similarity
non-traceable author statement
Biological Process non-traceable author statement
Cellular Component ( 0 terms) Sequence Ontology: Class of Gene Interactions & Pathways
Summary of Physical Interactions
protein-protein anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
experimental knowledge based
experimental knowledge based
anti tag coimmunoprecipitation, peptide massfingerprinting
experimental knowledge based
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
Summary of Genetic Interactions Interacts with Please look at the allele data
for full details of the genetic interactions
External Data Linkouts
- A database of protein and genetic interactions
- A comprehensive database of gene and protein interactions.
Protein-protein interactions (PPI) from both known and predicted PPI data sets.
- A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores.
Orthologs
OrthoDB Orthologs (51)
- based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
OrthoDB orthology group searches
Drosophila inclusive ortholog search
Dipteran inclusive ortholog search
Insect inclusive ortholog search
Arthropod inclusive ortholog search
Metazoa inclusive ortholog search
Orthologs in Drosophila Species
(EOG6G1N24 )
Drosophila melanogaster
fruit fly
Drosophila pseudoobscura pseudoobscura
Y
Orthologs in non-Drosophila Dipterans
(EOG6WDDM5 )
Aedes aegypti
Yellow fever mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects
(EOG6PVMV1 )
Apis mellifera
Western honey bee
Amel\GB55646
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG007972
Acromyrmex echinatior
Panamanian leafcutter ant
Aech\AECH10949
Atta cephalotes
Leafcutter ant
Acep\ACEP20098
Camponotus floridanus
Florida carpenter ant
Cflo\CFLO19331
Harpegnathos saltator
Jerdons jumping ant
Hsal\HSAL19778
Linepithema humile
Argentine ant
Lhum\LH11855
Pogonomyrmex barbatus
Red harvester ant
Pbar\PB26030
Acyrthosiphon pisum
Pea aphid
Pediculus humanus
Human body louse
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Orthologs in non-Insect Arthropods
(EOG6TDZ1M )
Ixodes scapularis
Deer tick
Orthologs in non-Arthropod Metazoa
(EOG6GJBGJ )
Caenorhabditis elegans
Nematode
Strongylocentrotus purpuratus
Purple sea urchin
Xenopus tropicalis
Western clawed frog
Xenopus tropicalis
Western clawed frog
Xenopus tropicalis
Western clawed frog
Gallus gallus
Domestic chicken
Rattus norvegicus
Norway rat
Rattus norvegicus
Norway rat
Rattus norvegicus
Norway rat
Rattus norvegicus
Norway rat
Rattus norvegicus
Norway rat
Rattus norvegicus
Norway rat
Human Orthologs (3)AAA Orthologs (11)
based on analysis using Dmel annotation version 4.3
Drosophila pseudoobscura pseudoobscura
Stocks & Reagents Stocks Listed in FlyBase ( 36 )Harvard VDRC Genomic Clones ( 2 )Please Note FlyBase no
longer curates genomic clone accessions so this list
may not be complete
cDNA Clones ( 291 )Please Note This section lists
cDNAs and ESTs that fall within the genomic extent
of the gene model, which may include cDNAs and ESTs
of genes within introns, or of overlapping genes.
Please see
GBrowse for alignment of the cDNAs and ESTs
to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones Other clones cDNA Clones, End Sequenced
(ESTs) BDGP DGC clones Other clones RNAi & Array Information Linkouts
- Results from RNAi screens.
- GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents
Antibody Information
Other Information Discoverer Etymology Identification Relationship to Other Genes Source for database identity of Source for identity of: gish CKI-related
Source for database merge of Source for merge of: CKI-related CG6963
Source for merge of: gish anon-WO0118547.425
Source for merge of: gish NEST:bs27c08
Additional comments Source for merge of gish anon-WO0118547.425 was sequence comparison (date:051113).
Other Comments The
gish product phosphorylates the
arr protein.
When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an increase in the proportion of S phase cells and/or aneuploidy, an increase in the proportion of G2/M phase cells, and increase in in mitotic index, and spindle abnormalities are seen.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
gish ,
so ,
ey and
hh act in the posterior region of the eye disc to prevent precocious glial cell migration.
Area matching Drosophila EST AA949934. This EST has sequence similarity to mammalian Casein kinase genes.
gish may be part of a repulsive signaling mechanism coordinating glial migration and neuronal development in the developing eye.
In mutants, glial cells prematurely migrate into the ectopic positions anterior to the photoreceptors. As a consequence, a subset of photoreceptor axons projects anteriorly, suggesting an instructive role for the glial cells in axon pathfinding. Furthermore,
gish mutant glial cells also inappropriately adhere to and migrate along Bolwigs nerve.
Postmeiotic differentiation defect.
External Crossreferences & Linkouts
Sequence Crossreferences
- A searchable database of RefSeq genes.
Other Crossreferences
domains - A database of protein families, domains, and functional sites
Linkouts
- A database of protein and genetic interactions
- A comprehensive database of gene and protein interactions.
- Results from RNAi screens.
- Cell culture data for RNAi and other high-throughput technologies
- Integrated genomics database for Drosophila, Anopheles, and C.elegans
- A curated knowledgebase of Drosophila melanogaster pathways
- GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents
Protein-protein interactions (PPI) from both known and predicted PPI data sets.
- Data generated by the modENCODE project.
- A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores.
Synonyms & Secondary IDs
( 19 )Reported As Symbol Synonym CG6963
(
Morin et al., 2001 ,
Brody et al., 2002 ,
Hummel, 2001.3.14 ,
Morrison et al., 2000 ,
Molnar et al., 2006 ,
Keleman et al., 2009.8.5 ,
Perkins et al., 2009 ,
Khanna et al., 2010 ,
Ni et al., 2011.7.19 ,
Weber et al., 2012 )
gish
(
Tan et al., 2010 ,
Liebl et al., 2006 ,
Nerusheva et al., 2007 ,
Jia et al., 2005 ,
Stuart et al., 2007 ,
Buszczak et al., 2007 ,
Molnar et al., 2006 ,
Jungreis et al., 2011 ,
Markstein et al., 2008 ,
Rathke et al., 2007 ,
Nerusheva et al., 2009 ,
Bozas et al., 2009 ,
Davidson et al., 2009 ,
Mattila et al., 2008 ,
Silicheva et al., 2010 ,
Jungreis et al., 2011 ,
Japanese National Institute of Genetics, 2012.5.21 ,
Gault et al., 2012 ,
Weber et al., 2012 ,
Gurudatta et al., 2012 )
Name Synonym gilgamesh
(
Tan et al., 2010 ,
Hummel, 2001.3.14 ,
Hummel et al., 2001 ,
Gregory et al., 2007 ,
Frescas et al., 2006 ,
Pandey et al., 2005 ,
Rathke et al., 2007 ,
Nerusheva et al., 2009 ,
Bozas et al., 2009 ,
Eddison et al., 2012 ,
Gault et al., 2012 ,
Weber et al., 2012 )
Secondary FlyBase
IDs FBgn0011253 FBgn0038411 FBgn0045173 FBgn0062017 References ( 93 )Generate a list of List References by type
Recent research papers ( 10 )
Daniels et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109(22): 8588--8593Multiscale diffusion in the mitotic Drosophila melanogaster syncytial blastoderm. [FBrf0218444] Eddison et al., 2012, PLoS ONE 7(12): e51684A genetic screen for olfactory habituation mutations in Drosophila: analysis of novel foraging alleles and an underlying neural circuit. [FBrf0220425] Gault et al., 2012, J. Cell Biol. 196(5): 605--621Drosophila CK1-γ, gilgamesh, controls PCP-mediated morphogenesis through regulation of vesicle trafficking. [FBrf0217630] Gurudatta et al., 2012, Dev. Biol. 369(1): 124--132The BEAF insulator regulates genes involved in cell polarity and neoplastic growth. [FBrf0219796] Shaukat et al., 2012, PLoS ONE 7(10): e47447A screen for selective killing of cells with chromosomal instability induced by a spindle checkpoint defect. [FBrf0219770] Weber et al., 2012, Genetics 191(1): 145--162Novel regulators of planar cell polarity: a genetic analysis in Drosophila. [FBrf0218210] Abreu-Blanco et al., 2011, J. Cell Biol. 193(3): 455--464Cell wound repair in Drosophila occurs through three distinct phases of membrane and cytoskeletal remodeling. [FBrf0213583] Fernandez-Gonzalez and Zallen, 2011, Phys. Biol. 8(4): 045005Oscillatory behaviors and hierarchical assembly of contractile structures in intercalating cells. [FBrf0214479] Jungreis et al., 2011, Genome Res. 21(12): 2096--2113Evidence of abundant stop codon readthrough in Drosophila and other metazoa. [FBrf0216845] Meng et al., 2011, Mol. Biol. Cell 22(16): 2834--2847Role for casein kinase 1 in the phosphorylation of Claspin on critical residues necessary for the activation of Chk1. [FBrf0216930]
Recent reviews (0)
All reviews listed in FlyBase were published before 2011