Gene Dmel\DNApol-α180

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General Information

Symbol

Dmel\DNApol-α180

Species

D. melanogaster

Name

DNA polymerase α 180kD

Annotation symbol

CG6349

Feature type

protein_coding_gene

FlyBase ID

FBgn0259113

Gene Model Status

Current

Stock availability

4 publicly available

Also Known As

polα, pol α, POLA, DNApol-α, DNApol-alpha180, DNApolα

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

93F2-93F2

Sequence location

3R:17,492,284..17,497,472 [-]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

The gene DNA polymerase α 180kD is referred to in FlyBase by the symbol Dmel\DNApol-α180 (CG6349, FBgn0259113). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: DNA-directed DNA polymerase activity. There is experimental evidence that it is involved in the biological process: DNA replication; neurogenesis. 8 alleles are reported. The phenotype of these alleles is annotated with: eye. It has one annotated transcript and one annotated polypeptide. Protein features are: DNA polymerase alpha catalytic subunit, N-terminal domain; DNA polymerase, palm domain; DNA-directed DNA polymerase, family B; DNA-directed DNA polymerase, family B, conserved site; DNA-directed DNA polymerase, family B, exonuclease domain; DNA-directed DNA polymerase, family B, multifunctional domain; DNA-directed DNA polymerase, family B, pol2; Ribonuclease H-like domain; Zinc finger, DNA-directed DNA polymerase, family B, alpha. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1633249_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of DNApol-alpha180. Gene sequence location is 3R:17492284..17497472.

User Contributed Data

Beta version, feedback welcome

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2013_03

FB2013_02

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Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

93F2-93F2

Limits computationally determined from genome sequence between P{PZ}InR⁰⁵⁵⁴⁵ and P{PZ}tsl⁰⁰⁶¹⁷

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

93D-93F

Mapped by recombination and deficiency analysis.

(Brodsky et al., 2000)

93F1-93F1

(determined by in situ hybridisation)

(Melov et al., 1992)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\DNApol-α180 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.47

DGC clone appears problematic: incomplete CDS (GM13460)

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

DNApol-α180-RA

FBtr0084115

NM_080488

4624

1488

Additional Transcript Data & Comments

Reported size (kB)

5.7 (northern blot)

(Hirose et al., 1991)

4.8 (northern blot)

(Melov et al., 1992)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

DNApol-α180-PA

FBpp0083514

169.9

1488

8.11

NP_536736

AAF55908

Additional Polypeptide Data & Comments

Reported size (kDa)

1505 (aa); 171 (kD)

(Hirose et al., 1991)

1490 (aa); 180 (kD)

(Melov et al., 1992)

183 (kD)

(Peck et al., 1992)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

DNA-directed DNA polymerase, family B, conserved site (IPR017964)

DNA-directed DNA polymerase, family B, pol2 (IPR004578)

DNA-directed DNA polymerase, family B, exonuclease domain (IPR006133)

DNA-directed DNA polymerase, family B, multifunctional domain (IPR006134)

DNA-directed DNA polymerase, family B (IPR006172)

Ribonuclease H-like domain (IPR012337)

Zinc finger, DNA-directed DNA polymerase, family B, alpha (IPR015088)

DNA polymerase alpha catalytic subunit, N-terminal domain (IPR024647)

DNA polymerase, palm domain (IPR023211)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P26019

UniProtKB/TrEMBL

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

protein binding site

FBsf0000160881
3R:17,497,594..17,497,626

bound_moiety=zen-XP
evidence=experimental

(Hirose et al., 1994, Bergman et al., 2005)

protein binding site

FBsf0000160882
3R:17,497,519..17,497,543

bound_moiety=Dref-XP
evidence=experimental

(Bergman et al., 2005, Hirose et al., 1993)

protein binding site

FBsf0000160883
3R:17,497,466..17,497,495

bound_moiety=Dref-XP
evidence=experimental

(Hirose et al., 1993, Bergman et al., 2005)

protein binding site

FBsf0000159814
3R:17,497,654..17,497,679

bound_moiety=Dref-XP
evidence=experimental

(Hirose et al., 1993, Bergman et al., 2005)

regulatory region

FBsf0000435113
3R:17,497,274..17,497,551

evidence=Reporter construct (in vitro)

(Hirose et al., 1994, Halfon, 2012.6.19)

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage | early

(Melov et al., 1992)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

oogenesis & adult stage | female

ovary

(Melov et al., 1992)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

first instar larval stage

(Melov et al., 1992)

adult stage & oogenesis

follicle cell

(Melov et al., 1992)

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage & oogenesis

nurse cell

• nucleus

(Melov et al., 1992)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage | early

(Melov et al., 1992)

pupal stage | early

(Melov et al., 1992)

Additional Descriptive Data

DNApol-alpha180 protein is observed in nuclei of nurse cells and follicle cells. During early stages of embryogenesis, DNApol-alpha180 protein is mainly nuclear although dispersal occurrs during mitotic phases of the cell cycle.

(Melov et al., 1992)

DNApol-α180 activity is highest in embryos, 2nd instar larvae, and adult females.

(Hirose et al., 1991)

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

alpha DNA polymerase:primase complex

inferred from direct assay

(Peck et al., 1992, Kaguni et al., 1983, Villani et al., 1980)

nucleus

inferred from direct assay

(Melov et al., 1992)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		112.6
Larval Midgut		23.2
Larval Hindgut		44.4
Larval Malpighian Tubules		13.7
Larval Fat Body		14.6
Larval Salivary Gland		88.5
Larval Trachea		48.85
Larval Carcass		53.475
Adult Head		11.6
Adult Eye		3.575
Adult Brain		4.8
Adult Thoracic-Abdominal Ganglion		7.6
Adult Crop		7.4
Adult Midgut		21.8
Adult Hindgut		16.2
Adult Malpighian Tubules		6.7
Adult Fat Body		12.1
Adult Salivary Gland		6
Adult Heart		2.55
Adult VirginFemale Spermatheca		11.5
Adult InseminatedFemale Spermatheca		8.2
Adult Ovary		218.8
Adult Testis		14.5
Adult Male Accessory Gland		8.7
Adult Carcass		15.3
Expression Level Scale		None Low Moderate

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		112.6
Larval Midgut		23.2
Larval Hindgut		44.4
Larval Malpighian Tubules		13.7
Larval Fat Body		14.6
Larval Salivary Gland		88.5
Larval Trachea		48.85
Larval Carcass		53.475
Adult Head		11.6
Adult Eye		3.575
Adult Brain		4.8
Adult Thoracic-Abdominal Ganglion		7.6
Adult Crop		7.4
Adult Midgut		21.8
Adult Hindgut		16.2
Adult Malpighian Tubules		6.7
Adult Fat Body		12.1
Adult Salivary Gland		6
Adult Heart		2.55
Adult VirginFemale Spermatheca		11.5
Adult InseminatedFemale Spermatheca		8.2
Adult Ovary		218.8
Adult Testis		14.5
Adult Male Accessory Gland		8.7
Adult Carcass		15.3
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		112.6
Larval Midgut		23.2
Larval Hindgut		44.4
Larval Malpighian Tubules		13.7
Larval Fat Body		14.6
Larval Salivary Gland		88.5
Larval Trachea		48.85
Larval Carcass		53.475
Adult Head		11.6
Adult Eye		3.575
Adult Brain		4.8
Adult Thoracic-Abdominal Ganglion		7.6
Adult Crop		7.4
Adult Midgut		21.8
Adult Hindgut		16.2
Adult Malpighian Tubules		6.7
Adult Fat Body		12.1
Adult Salivary Gland		6
Adult Heart		2.55
Adult VirginFemale Spermatheca		11.5
Adult InseminatedFemale Spermatheca		8.2
Adult Ovary		218.8
Adult Testis		14.5
Adult Male Accessory Gland		8.7
Adult Carcass		15.3
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		112.6
Larval Midgut		23.2
Larval Hindgut		44.4
Larval Malpighian Tubules		13.7
Larval Fat Body		14.6
Larval Salivary Gland		88.5
Larval Trachea		48.85
Larval Carcass		53.475
Adult Head		11.6
Adult Eye		3.575
Adult Brain		4.8
Adult Thoracic-Abdominal Ganglion		7.6
Adult Crop		7.4
Adult Midgut		21.8
Adult Hindgut		16.2
Adult Malpighian Tubules		6.7
Adult Fat Body		12.1
Adult Salivary Gland		6
Adult Heart		2.55
Adult VirginFemale Spermatheca		11.5
Adult InseminatedFemale Spermatheca		8.2
Adult Ovary		218.8
Adult Testis		14.5
Adult Male Accessory Gland		8.7
Adult Carcass		15.3
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		112.6
Larval Midgut		23.2
Larval Hindgut		44.4
Larval Malpighian Tubules		13.7
Larval Fat Body		14.6
Larval Salivary Gland		88.5
Larval Trachea		48.85
Larval Carcass		53.475
Adult Head		11.6
Adult Eye		3.575
Adult Brain		4.8
Adult Thoracic-Abdominal Ganglion		7.6
Adult Crop		7.4
Adult Midgut		21.8
Adult Hindgut		16.2
Adult Malpighian Tubules		6.7
Adult Fat Body		12.1
Adult Salivary Gland		6
Adult Heart		2.55
Adult VirginFemale Spermatheca		11.5
Adult InseminatedFemale Spermatheca		8.2
Adult Ovary		218.8
Adult Testis		14.5
Adult Male Accessory Gland		8.7
Adult Carcass		15.3
Expression Level Scale		None Low Moderate

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		112.6
Larval Midgut		23.2
Larval Hindgut		44.4
Larval Malpighian Tubules		13.7
Larval Fat Body		14.6
Larval Salivary Gland		88.5
Larval Trachea		48.85
Larval Carcass		53.475
Adult Head		11.6
Adult Eye		3.575
Adult Brain		4.8
Adult Thoracic-Abdominal Ganglion		7.6
Adult Crop		7.4
Adult Midgut		21.8
Adult Hindgut		16.2
Adult Malpighian Tubules		6.7
Adult Fat Body		12.1
Adult Salivary Gland		6
Adult Heart		2.55
Adult VirginFemale Spermatheca		11.5
Adult InseminatedFemale Spermatheca		8.2
Adult Ovary		218.8
Adult Testis		14.5
Adult Male Accessory Gland		8.7
Adult Carcass		15.3
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		112.6
Larval Midgut		23.2
Larval Hindgut		44.4
Larval Malpighian Tubules		13.7
Larval Fat Body		14.6
Larval Salivary Gland		88.5
Larval Trachea		48.85
Larval Carcass		53.475
Adult Head		11.6
Adult Eye		3.575
Adult Brain		4.8
Adult Thoracic-Abdominal Ganglion		7.6
Adult Crop		7.4
Adult Midgut		21.8
Adult Hindgut		16.2
Adult Malpighian Tubules		6.7
Adult Fat Body		12.1
Adult Salivary Gland		6
Adult Heart		2.55
Adult VirginFemale Spermatheca		11.5
Adult InseminatedFemale Spermatheca		8.2
Adult Ovary		218.8
Adult Testis		14.5
Adult Male Accessory Gland		8.7
Adult Carcass		15.3
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		112.6
Larval Midgut		23.2
Larval Hindgut		44.4
Larval Malpighian Tubules		13.7
Larval Fat Body		14.6
Larval Salivary Gland		88.5
Larval Trachea		48.85
Larval Carcass		53.475
Adult Head		11.6
Adult Eye		3.575
Adult Brain		4.8
Adult Thoracic-Abdominal Ganglion		7.6
Adult Crop		7.4
Adult Midgut		21.8
Adult Hindgut		16.2
Adult Malpighian Tubules		6.7
Adult Fat Body		12.1
Adult Salivary Gland		6
Adult Heart		2.55
Adult VirginFemale Spermatheca		11.5
Adult InseminatedFemale Spermatheca		8.2
Adult Ovary		218.8
Adult Testis		14.5
Adult Male Accessory Gland		8.7
Adult Carcass		15.3
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	no informative data
NA	Eye	3.575
NA	Brain	4.8
112.6	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	7.6
NA	Crop	7.4
no informative data	Midgut	no informative data
44.4	Hindgut	no informative data
no informative data	Malpighian Tubules	6.7
no informative data	Fat Body	no informative data
88.5	Salivary Gland	6
NA	Heart	2.55
48.85	Trachea	NA
NA	VirginFemale Spermatheca	no informative data
NA	InseminatedFemale Spermatheca	8.2
NA	Ovary	218.8
NA	Testis	no informative data
NA	Male Accessory Gland	8.7
53.475	Carcass	no informative data

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		9
central nervous system, larvae L3		8
central nervous system, pupae P8		0
head, virgin 1-day female		1
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		1
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		2
digestive system, 1-day adult		1
digestive system, 4-day adult		2
digestive system, 20-day adult		1
fat body, larvae L3 wandering		1
fat body, white prepupae		2
fat body, pupae P8		0
carcass, larvae L3 wandering		6
carcass, 1-day adult		1
carcass, 4-day adult		2
carcass, 20-day adult		1
ovary, virgin 4-day female		49
ovary, mated 4-day female		44
testis, mated 4-day male		1
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		9
central nervous system, larvae L3		8
central nervous system, pupae P8		0
head, virgin 1-day female		1
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		1
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		2
digestive system, 1-day adult		1
digestive system, 4-day adult		2
digestive system, 20-day adult		1
fat body, larvae L3 wandering		1
fat body, white prepupae		2
fat body, pupae P8		0
carcass, larvae L3 wandering		6
carcass, 1-day adult		1
carcass, 4-day adult		2
carcass, 20-day adult		1
ovary, virgin 4-day female		(49)
ovary, mated 4-day female		(44)
testis, mated 4-day male		1
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		9
central nervous system, larvae L3		8
central nervous system, pupae P8		0
head, virgin 1-day female		1
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		1
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		2
digestive system, 1-day adult		1
digestive system, 4-day adult		2
digestive system, 20-day adult		1
fat body, larvae L3 wandering		1
fat body, white prepupae		2
fat body, pupae P8		0
carcass, larvae L3 wandering		6
carcass, 1-day adult		1
carcass, 4-day adult		2
carcass, 20-day adult		1
ovary, virgin 4-day female		49
ovary, mated 4-day female		44
testis, mated 4-day male		1
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		9
central nervous system, larvae L3		8
central nervous system, pupae P8		0
head, virgin 1-day female		1
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		1
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		2
digestive system, 1-day adult		1
digestive system, 4-day adult		2
digestive system, 20-day adult		1
fat body, larvae L3 wandering		1
fat body, white prepupae		2
fat body, pupae P8		0
carcass, larvae L3 wandering		6
carcass, 1-day adult		1
carcass, 4-day adult		2
carcass, 20-day adult		1
ovary, virgin 4-day female		49
ovary, mated 4-day female		44
testis, mated 4-day male		1
accessory gland, mated 4-day male		0
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		9
central nervous system, larvae L3		8
central nervous system, pupae P8		0
head, virgin 1-day female		1
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		1
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		2
digestive system, 1-day adult		1
digestive system, 4-day adult		2
digestive system, 20-day adult		1
fat body, larvae L3 wandering		1
fat body, white prepupae		2
fat body, pupae P8		0
carcass, larvae L3 wandering		6
carcass, 1-day adult		1
carcass, 4-day adult		2
carcass, 20-day adult		1
ovary, virgin 4-day female		49
ovary, mated 4-day female		44
testis, mated 4-day male		1
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		9
central nervous system, larvae L3		8
central nervous system, pupae P8		0
head, virgin 1-day female		1
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		1
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		2
digestive system, 1-day adult		1
digestive system, 4-day adult		2
digestive system, 20-day adult		1
fat body, larvae L3 wandering		1
fat body, white prepupae		2
fat body, pupae P8		0
carcass, larvae L3 wandering		6
carcass, 1-day adult		1
carcass, 4-day adult		2
carcass, 20-day adult		1
ovary, virgin 4-day female		(49)
ovary, mated 4-day female		(44)
testis, mated 4-day male		1
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		9
central nervous system, larvae L3		8
central nervous system, pupae P8		0
head, virgin 1-day female		1
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		1
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		2
digestive system, 1-day adult		1
digestive system, 4-day adult		2
digestive system, 20-day adult		1
fat body, larvae L3 wandering		1
fat body, white prepupae		2
fat body, pupae P8		0
carcass, larvae L3 wandering		6
carcass, 1-day adult		1
carcass, 4-day adult		2
carcass, 20-day adult		1
ovary, virgin 4-day female		49
ovary, mated 4-day female		44
testis, mated 4-day male		1
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		9
central nervous system, larvae L3		8
central nervous system, pupae P8		0
head, virgin 1-day female		1
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		1
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		1
digestive system, larvae L3 wandering		2
digestive system, 1-day adult		1
digestive system, 4-day adult		2
digestive system, 20-day adult		1
fat body, larvae L3 wandering		1
fat body, white prepupae		2
fat body, pupae P8		0
carcass, larvae L3 wandering		6
carcass, 1-day adult		1
carcass, 4-day adult		2
carcass, 20-day adult		1
ovary, virgin 4-day female		49
ovary, mated 4-day female		44
testis, mated 4-day male		1
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		35
embryo 02-04hr		26
embryo 04-06hr		59
embryo 06-08hr		28
embryo 08-10hr		16
embryo 10-12hr		5
embryo 12-14hr		5
embryo 14-16hr		4
embryo 16-18hr		3
embryo 18-20hr		2
embryo 20-22hr		1
embryo 22-24hr		2
larva L1		5
larva L2		6
larva L3 12hr old		4
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		5
larva L3 puffstage 7-9		5
white prepupae new		5
white prepupae 12hr		8
white prepupae 24hr		5
pupae 2d postWPP		1
pupae 3d postWPP		1
pupae 4d postWPP		2
adult male 01day		1
adult male 05day		1
adult male 30day		1
adult female 01day		11
adult female 05day		26
adult female 30day		24
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(35)
embryo 02-04hr		26
embryo 04-06hr		(59)
embryo 06-08hr		(28)
embryo 08-10hr		16
embryo 10-12hr		5
embryo 12-14hr		5
embryo 14-16hr		4
embryo 16-18hr		3
embryo 18-20hr		2
embryo 20-22hr		1
embryo 22-24hr		2
larva L1		5
larva L2		6
larva L3 12hr old		4
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		5
larva L3 puffstage 7-9		5
white prepupae new		5
white prepupae 12hr		8
white prepupae 24hr		5
pupae 2d postWPP		1
pupae 3d postWPP		1
pupae 4d postWPP		2
adult male 01day		1
adult male 05day		1
adult male 30day		1
adult female 01day		11
adult female 05day		26
adult female 30day		24
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		35
embryo 02-04hr		26
embryo 04-06hr		59
embryo 06-08hr		28
embryo 08-10hr		16
embryo 10-12hr		5
embryo 12-14hr		5
embryo 14-16hr		4
embryo 16-18hr		3
embryo 18-20hr		2
embryo 20-22hr		1
embryo 22-24hr		2
larva L1		5
larva L2		6
larva L3 12hr old		4
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		5
larva L3 puffstage 7-9		5
white prepupae new		5
white prepupae 12hr		8
white prepupae 24hr		5
pupae 2d postWPP		1
pupae 3d postWPP		1
pupae 4d postWPP		2
adult male 01day		1
adult male 05day		1
adult male 30day		1
adult female 01day		11
adult female 05day		26
adult female 30day		24
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		35
embryo 02-04hr		26
embryo 04-06hr		59
embryo 06-08hr		28
embryo 08-10hr		16
embryo 10-12hr		5
embryo 12-14hr		5
embryo 14-16hr		4
embryo 16-18hr		3
embryo 18-20hr		2
embryo 20-22hr		1
embryo 22-24hr		2
larva L1		5
larva L2		6
larva L3 12hr old		4
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		5
larva L3 puffstage 7-9		5
white prepupae new		5
white prepupae 12hr		8
white prepupae 24hr		5
pupae 2d postWPP		1
pupae 3d postWPP		1
pupae 4d postWPP		2
adult male 01day		1
adult male 05day		1
adult male 30day		1
adult female 01day		11
adult female 05day		26
adult female 30day		24
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		35
embryo 02-04hr		26
embryo 04-06hr		59
embryo 06-08hr		28
embryo 08-10hr		16
embryo 10-12hr		5
embryo 12-14hr		5
embryo 14-16hr		4
embryo 16-18hr		3
embryo 18-20hr		2
embryo 20-22hr		1
embryo 22-24hr		2
larva L1		5
larva L2		6
larva L3 12hr old		4
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		5
larva L3 puffstage 7-9		5
white prepupae new		5
white prepupae 12hr		8
white prepupae 24hr		5
pupae 2d postWPP		1
pupae 3d postWPP		1
pupae 4d postWPP		2
adult male 01day		1
adult male 05day		1
adult male 30day		1
adult female 01day		11
adult female 05day		26
adult female 30day		24
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(35)
embryo 02-04hr		26
embryo 04-06hr		(59)
embryo 06-08hr		28
embryo 08-10hr		16
embryo 10-12hr		5
embryo 12-14hr		5
embryo 14-16hr		4
embryo 16-18hr		3
embryo 18-20hr		2
embryo 20-22hr		1
embryo 22-24hr		2
larva L1		5
larva L2		6
larva L3 12hr old		4
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		5
larva L3 puffstage 7-9		5
white prepupae new		5
white prepupae 12hr		8
white prepupae 24hr		5
pupae 2d postWPP		1
pupae 3d postWPP		1
pupae 4d postWPP		2
adult male 01day		1
adult male 05day		1
adult male 30day		1
adult female 01day		11
adult female 05day		26
adult female 30day		24
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		35
embryo 02-04hr		26
embryo 04-06hr		59
embryo 06-08hr		28
embryo 08-10hr		16
embryo 10-12hr		5
embryo 12-14hr		5
embryo 14-16hr		4
embryo 16-18hr		3
embryo 18-20hr		2
embryo 20-22hr		1
embryo 22-24hr		2
larva L1		5
larva L2		6
larva L3 12hr old		4
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		5
larva L3 puffstage 7-9		5
white prepupae new		5
white prepupae 12hr		8
white prepupae 24hr		5
pupae 2d postWPP		1
pupae 3d postWPP		1
pupae 4d postWPP		2
adult male 01day		1
adult male 05day		1
adult male 30day		1
adult female 01day		11
adult female 05day		26
adult female 30day		24
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		35
embryo 02-04hr		26
embryo 04-06hr		59
embryo 06-08hr		28
embryo 08-10hr		16
embryo 10-12hr		5
embryo 12-14hr		5
embryo 14-16hr		4
embryo 16-18hr		3
embryo 18-20hr		2
embryo 20-22hr		1
embryo 22-24hr		2
larva L1		5
larva L2		6
larva L3 12hr old		4
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		5
larva L3 puffstage 7-9		5
white prepupae new		5
white prepupae 12hr		8
white prepupae 24hr		5
pupae 2d postWPP		1
pupae 3d postWPP		1
pupae 4d postWPP		2
adult male 01day		1
adult male 05day		1
adult male 30day		1
adult female 01day		11
adult female 05day		26
adult female 30day		24
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		27
Schneider line 2 Sg4		8
embryonic 1182-4H		12
embryonic GM2		22
embryonic Kc167		14
embryonic S1		10
embryonic S3		17
leg disc CME L1		8
wing disc CME-W2		11
wing disc ML-DmD8		13
wing disc ML-DmD9		15
wing disc ML-DmD16-c3		18
wing disc ML-DmD21		4
wing disc ML-DmD32		10
haltere disc ML-DmD17-c3		30
eye-antennal disc ML-DmD11		12
antennal disc ML-DmD20-c5		10
mixed discs ML-DmD4-c1		8
CNS ML-DmBG1-c1		20
CNS ML-DmBG2-c2		11
tumorous blood cells mbn2		9
ovary fGS/OSS		80
ovary OSC		25
ovary OSS		18
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(27)
Schneider line 2 Sg4		8
embryonic 1182-4H		12
embryonic GM2		22
embryonic Kc167		14
embryonic S1		10
embryonic S3		17
leg disc CME L1		8
wing disc CME-W2		11
wing disc ML-DmD8		13
wing disc ML-DmD9		15
wing disc ML-DmD16-c3		18
wing disc ML-DmD21		4
wing disc ML-DmD32		10
haltere disc ML-DmD17-c3		(30)
eye-antennal disc ML-DmD11		12
antennal disc ML-DmD20-c5		10
mixed discs ML-DmD4-c1		8
CNS ML-DmBG1-c1		20
CNS ML-DmBG2-c2		11
tumorous blood cells mbn2		9
ovary fGS/OSS		(80)
ovary OSC		25
ovary OSS		18
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		27
Schneider line 2 Sg4		8
embryonic 1182-4H		12
embryonic GM2		22
embryonic Kc167		14
embryonic S1		10
embryonic S3		17
leg disc CME L1		8
wing disc CME-W2		11
wing disc ML-DmD8		13
wing disc ML-DmD9		15
wing disc ML-DmD16-c3		18
wing disc ML-DmD21		4
wing disc ML-DmD32		10
haltere disc ML-DmD17-c3		30
eye-antennal disc ML-DmD11		12
antennal disc ML-DmD20-c5		10
mixed discs ML-DmD4-c1		8
CNS ML-DmBG1-c1		20
CNS ML-DmBG2-c2		11
tumorous blood cells mbn2		9
ovary fGS/OSS		80
ovary OSC		25
ovary OSS		18
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		27
Schneider line 2 Sg4		8
embryonic 1182-4H		12
embryonic GM2		22
embryonic Kc167		14
embryonic S1		10
embryonic S3		17
leg disc CME L1		8
wing disc CME-W2		11
wing disc ML-DmD8		13
wing disc ML-DmD9		15
wing disc ML-DmD16-c3		18
wing disc ML-DmD21		4
wing disc ML-DmD32		10
haltere disc ML-DmD17-c3		30
eye-antennal disc ML-DmD11		12
antennal disc ML-DmD20-c5		10
mixed discs ML-DmD4-c1		8
CNS ML-DmBG1-c1		20
CNS ML-DmBG2-c2		11
tumorous blood cells mbn2		9
ovary fGS/OSS		80
ovary OSC		25
ovary OSS		18
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		27
Schneider line 2 Sg4		8
embryonic 1182-4H		12
embryonic GM2		22
embryonic Kc167		14
embryonic S1		10
embryonic S3		17
leg disc CME L1		8
wing disc CME-W2		11
wing disc ML-DmD8		13
wing disc ML-DmD9		15
wing disc ML-DmD16-c3		18
wing disc ML-DmD21		4
wing disc ML-DmD32		10
haltere disc ML-DmD17-c3		30
eye-antennal disc ML-DmD11		12
antennal disc ML-DmD20-c5		10
mixed discs ML-DmD4-c1		8
CNS ML-DmBG1-c1		20
CNS ML-DmBG2-c2		11
tumorous blood cells mbn2		9
ovary fGS/OSS		80
ovary OSC		25
ovary OSS		18
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		27
Schneider line 2 Sg4		8
embryonic 1182-4H		12
embryonic GM2		22
embryonic Kc167		14
embryonic S1		10
embryonic S3		17
leg disc CME L1		8
wing disc CME-W2		11
wing disc ML-DmD8		13
wing disc ML-DmD9		15
wing disc ML-DmD16-c3		18
wing disc ML-DmD21		4
wing disc ML-DmD32		10
haltere disc ML-DmD17-c3		30
eye-antennal disc ML-DmD11		12
antennal disc ML-DmD20-c5		10
mixed discs ML-DmD4-c1		8
CNS ML-DmBG1-c1		20
CNS ML-DmBG2-c2		11
tumorous blood cells mbn2		9
ovary fGS/OSS		(80)
ovary OSC		25
ovary OSS		18
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		27
Schneider line 2 Sg4		8
embryonic 1182-4H		12
embryonic GM2		22
embryonic Kc167		14
embryonic S1		10
embryonic S3		17
leg disc CME L1		8
wing disc CME-W2		11
wing disc ML-DmD8		13
wing disc ML-DmD9		15
wing disc ML-DmD16-c3		18
wing disc ML-DmD21		4
wing disc ML-DmD32		10
haltere disc ML-DmD17-c3		30
eye-antennal disc ML-DmD11		12
antennal disc ML-DmD20-c5		10
mixed discs ML-DmD4-c1		8
CNS ML-DmBG1-c1		20
CNS ML-DmBG2-c2		11
tumorous blood cells mbn2		9
ovary fGS/OSS		80
ovary OSC		25
ovary OSS		18
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		27
Schneider line 2 Sg4		8
embryonic 1182-4H		12
embryonic GM2		22
embryonic Kc167		14
embryonic S1		10
embryonic S3		17
leg disc CME L1		8
wing disc CME-W2		11
wing disc ML-DmD8		13
wing disc ML-DmD9		15
wing disc ML-DmD16-c3		18
wing disc ML-DmD21		4
wing disc ML-DmD32		10
haltere disc ML-DmD17-c3		30
eye-antennal disc ML-DmD11		12
antennal disc ML-DmD20-c5		10
mixed discs ML-DmD4-c1		8
CNS ML-DmBG1-c1		20
CNS ML-DmBG2-c2		11
tumorous blood cells mbn2		9
ovary fGS/OSS		80
ovary OSC		25
ovary OSS		18
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		6
cold shock, 4-day adult		6
heat shock, 4-day adult		10
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		2
Cadmium 50 mM 48 hrs, 4-day adult		12
Cadmium 100 mM 48 hrs, 4-day adult		27
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		19
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		7
Ethanol 2.5% 3 hrs, larvae L3		25
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		22
Caffeine 25 mg/ml 48 hrs, 4-day adult		8
Paraquat 5 mM 48 hrs, 4-day adult		17
Paraquat 10 mM 48 hrs, 4-day adult		13
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		6
cold shock, 4-day adult		6
heat shock, 4-day adult		10
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		2
Cadmium 50 mM 48 hrs, 4-day adult		12
Cadmium 100 mM 48 hrs, 4-day adult		(27)
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		19
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		7
Ethanol 2.5% 3 hrs, larvae L3		25
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		22
Caffeine 25 mg/ml 48 hrs, 4-day adult		8
Paraquat 5 mM 48 hrs, 4-day adult		17
Paraquat 10 mM 48 hrs, 4-day adult		13
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		6
cold shock, 4-day adult		6
heat shock, 4-day adult		10
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		2
Cadmium 50 mM 48 hrs, 4-day adult		12
Cadmium 100 mM 48 hrs, 4-day adult		27
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		19
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		7
Ethanol 2.5% 3 hrs, larvae L3		25
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		22
Caffeine 25 mg/ml 48 hrs, 4-day adult		8
Paraquat 5 mM 48 hrs, 4-day adult		17
Paraquat 10 mM 48 hrs, 4-day adult		13
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		6
cold shock, 4-day adult		6
heat shock, 4-day adult		10
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		2
Cadmium 50 mM 48 hrs, 4-day adult		12
Cadmium 100 mM 48 hrs, 4-day adult		27
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		19
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		7
Ethanol 2.5% 3 hrs, larvae L3		25
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		22
Caffeine 25 mg/ml 48 hrs, 4-day adult		8
Paraquat 5 mM 48 hrs, 4-day adult		17
Paraquat 10 mM 48 hrs, 4-day adult		13
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		6
cold shock, 4-day adult		6
heat shock, 4-day adult		10
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		2
Cadmium 50 mM 48 hrs, 4-day adult		12
Cadmium 100 mM 48 hrs, 4-day adult		27
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		19
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		7
Ethanol 2.5% 3 hrs, larvae L3		25
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		22
Caffeine 25 mg/ml 48 hrs, 4-day adult		8
Paraquat 5 mM 48 hrs, 4-day adult		17
Paraquat 10 mM 48 hrs, 4-day adult		13
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		6
cold shock, 4-day adult		6
heat shock, 4-day adult		10
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		2
Cadmium 50 mM 48 hrs, 4-day adult		12
Cadmium 100 mM 48 hrs, 4-day adult		27
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		19
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		7
Ethanol 2.5% 3 hrs, larvae L3		25
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		22
Caffeine 25 mg/ml 48 hrs, 4-day adult		8
Paraquat 5 mM 48 hrs, 4-day adult		17
Paraquat 10 mM 48 hrs, 4-day adult		13
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		6
cold shock, 4-day adult		6
heat shock, 4-day adult		10
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		2
Cadmium 50 mM 48 hrs, 4-day adult		12
Cadmium 100 mM 48 hrs, 4-day adult		27
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		19
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		7
Ethanol 2.5% 3 hrs, larvae L3		25
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		22
Caffeine 25 mg/ml 48 hrs, 4-day adult		8
Paraquat 5 mM 48 hrs, 4-day adult		17
Paraquat 10 mM 48 hrs, 4-day adult		13
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		6
cold shock, 4-day adult		6
heat shock, 4-day adult		10
Cadmium 50 mM 6 hrs, larvae L3		2
Cadmium 50 mM 12 hrs, larvae L3		2
Cadmium 50 mM 48 hrs, 4-day adult		12
Cadmium 100 mM 48 hrs, 4-day adult		27
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		19
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		7
Ethanol 2.5% 3 hrs, larvae L3		25
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		2
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		22
Caffeine 25 mg/ml 48 hrs, 4-day adult		8
Paraquat 5 mM 48 hrs, 4-day adult		17
Paraquat 10 mM 48 hrs, 4-day adult		13
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010, Graveley et al., 2011)

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0259113

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal

DNApol-α180^GD4238

lethal (with DNApol-α180^Emus304)

lethal (with DNApol-α180^Q1132ter)

DNApol-α180^Emus304

lethal (with DNApol-α180^Q1322ter)

DNApol-α180^Emus304

lethal | embryonic stage | recessive

DNApol-α180^Emus304

lethal | pupal stage, with Scer\GAL4^pnr-MD237

DNApol-α180^GD4238

lethal | recessive

DNApol-α180^Emus304

Other Phenotypes

Allele

neuroanatomy defective | larval stage

DNApol-α180^GD4238

visible, with Scer\GAL4^ey.PU

DNApol-α180^NIG.6349R

Phenotype manifest in

Allele

embryonic/larval neuroblast | larval stage

DNApol-α180^GD4238

eye, with Scer\GAL4^ey.PU

DNApol-α180^NIG.6349R

ganglion mother cell | larval stage

DNApol-α180^GD4238

Classical Alleles ( 5 )

For All Classical Alleles Show

Allele of DNApol-α180	Mutagen	Stocks	Known lesion
DNApol-α180^G13925	P-element activity	1	--
DNApol-α180^NP0280		1	--
DNApol-α180^Emus304	spontaneous	0	Yes
DNApol-α180^Q1132ter	ethyl methanesulfonate	0	Yes
DNApol-α180^Q1322ter	ethyl methanesulfonate	0	Yes

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of DNApol-α180	Mutagen	Stocks	Known lesion
DNApol-α180^GD4238	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
DNApol-α180^KK100063	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
DNApol-α180^NIG.6349R	in vitro construct - regulatory fusion in vitro construct - RNAi	0	Yes

Aneuploid Aberrations

Disrupted in

Df(3R)Exel6186

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

reporter construct

P{5'-358DpolαlacZ}

P{5'-358DpolαlacZmut1}

P{5'-358DpolαlacZmut1+2+3}

P{5'-358DpolαlacZmut2}

P{5'-358DpolαlacZmut2+3}

P{5'-358DpolαlacZmut3}

Insertions

Type of insertions

Name

Expression data

insertion of enhancer trap binary system

P{GawB}DNApol-α180^NP0280

insertion of mobile activating element

P{EP}DNApol-α180^G13925

Gene Ontology: Function, Process & Cellular Component ( 10 unique terms )

Terms Based on Experimental Evidence ( 5 terms )

Molecular Function

CV term

Evidence

References

DNA-directed DNA polymerase activity

inferred from direct assay

(Peck et al., 1992, Kaguni et al., 1983, Villani et al., 1980)

Biological Process

CV term

Evidence

References

DNA replication

inferred from direct assay

(Kaguni et al., 1983, Villani et al., 1980)

neurogenesis

inferred from mutant phenotype

(Neumüller et al., 2011)

Cellular Component

CV term

Evidence

References

alpha DNA polymerase:primase complex

inferred from direct assay

(Peck et al., 1992, Kaguni et al., 1983, Villani et al., 1980)

nucleus

inferred from direct assay

(Melov et al., 1992)

Terms Based on Predictions or Assertions ( 7 terms )

Molecular Function

CV term

Evidence

References

3'-5'-exodeoxyribonuclease activity

non-traceable author statement

(Swiss-Prot Project Members, 1992.5.1)

DNA binding

inferred from electronic annotation with InterPro:IPR004578, InterPro:IPR006134

(FlyBase Curators et al., 2004-)

DNA-directed DNA polymerase activity

inferred from sequence or structural similarity with UniProtKB:P09884

(FlyBase, 1992-)

non-traceable author statement

(Burgers et al., 2001, Swiss-Prot Project Members, 1992.5.1)

nucleoside binding

inferred from electronic annotation with InterPro:IPR015088

(FlyBase Curators et al., 2004-)

nucleotide binding

inferred from electronic annotation with InterPro:IPR004578, InterPro:IPR006134, InterPro:IPR006172, InterPro:IPR017964

(FlyBase Curators et al., 2004-)

Biological Process

CV term

Evidence

References

DNA strand elongation involved in DNA replication

non-traceable author statement

(Swiss-Prot Project Members, 1992.5.1)

Cellular Component

CV term

Evidence

References

alpha DNA polymerase:primase complex

inferred from sequence or structural similarity with UniProtKB:P09884

(FlyBase, 1992-)

non-traceable author statement

(Swiss-Prot Project Members, 1992.5.1)

Sequence Ontology: Class of Gene

gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

protein-protein

Interacting group

Assay

References

DNApol-α180 - CG5190

anti tag coimmunoprecipitation, peptide massfingerprinting

(Mintseris et al., 2009.11.23)

DNApol-α180 - Ctf4

two hybrid

(Gosnell and Christensen, 2011)

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

DNApol-α180 allele

Gene

References

E(mus304)¹

mei-41

(Brodsky et al., 2000)

mus304

(Brodsky et al., 2000)

unspecified

mei-41

(LaRocque et al., 2007)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions

•

67519

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0259113

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

42553

Orthologs

OrthoDB Orthologs (42) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG6QJS6H

Dipteran inclusive ortholog search

EOG6VQ9X6

Insect inclusive ortholog search

EOG6BRVFH

Arthropod inclusive ortholog search

EOG69P8D9

Metazoa inclusive ortholog search

EOG6RC3B8

Orthologs in Drosophila Species (EOG6QJS6H)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA19530

Drosophila persimilis

Dper\GL21505

Drosophila willistoni

Dwil\GK12074

Drosophila virilis

Dvir\GJ11070

Drosophila mojavensis

Dmoj\GI10263

Drosophila grimshawi

Dgri\GH17298

Orthologs in non-Drosophila Dipterans (EOG6VQ9X6)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL008247

Anopheles gambiae

Malaria mosquito

Agam\AGAP001929

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ015260

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ015261

Orthologs in non-Dipteran Insects (EOG6BRVFH)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\GB54600

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG019447

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG014422

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH10230

Atta cephalotes

Leafcutter ant

Acep\ACEP20807

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO15611

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL19798

Linepithema humile

Argentine ant

Lhum\LH23420

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB17252

Solenopsis invicta

Red fire ant

Sinv\SINV12453

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI072830

Bombyx mori

Silkmoth

Bmor\BGIBMGA003396

Pediculus humanus

Human body louse

Phum\PHUM210770

Tribolium castaneum

Red flour beetle

Tcas\DNApol-alpha180

Orthologs in non-Insect Arthropods (EOG69P8D9)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\Mblac1

Ixodes scapularis

Deer tick

Isca\ISCW012960

Orthologs in non-Arthropod Metazoa (EOG6RC3B8)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Caenorhabditis elegans

Nematode

Cele\Y47D3A.29

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_000210gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_023359gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_023010gn

Danio rerio

Zebrafish

Drer\pola1

Xenopus tropicalis

Western clawed frog

Xtro\pola1

Gallus gallus

Domestic chicken

Ggal\POLA1

Mus musculus

House mouse

Mmus\Pola1

Rattus norvegicus

Norway rat

Rnor\Pola1

Homo sapiens

Human

Hsap\POLA1

Human Orthologs (1)

Gene

OMIM

HGNC

Hsap\POLA1

AAA Orthologs (11) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA19530

Drosophila persimilis

Dper\GL21505

Drosophila willistoni

Dwil\GK12074

Drosophila virilis

Dvir\GJ11070

Drosophila mojavensis

Dmoj\GI10263

Drosophila grimshawi

Dgri\GH17298

Stocks & Reagents

Stocks Listed in FlyBase ( 4 )

Bloomington

31805

w¹¹¹⁸; P{EP}DNApol-α180^G13925/TM6C, Sb¹

Kyoto

103575

w^*; P{GawB}DNApol-α180^NP0280 / TM6C, Sb¹

VDRC

v103699

P{KK100063}VIE-260B

v11227

w¹¹¹⁸ P{GD4238}v11227

Genomic Clones ( 1 )

BACR16N15

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 17 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

GM13460

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Linkouts

DRSC - Results from RNAi screens.

•

FBgn0259113

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

42553

Antibody Information

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: DNApol-α180 E(mus304)

(LaRocque et al., 2007)

Additional comments

Other Comments

Reducing DNApol-α180 levels by itself des not result in any defects, while reducing DNApol-α180 levels in the absence of mei-41 results in elevated p53-dependent apoptosis, rough eyes and increased genomic instability.

(LaRocque et al., 2007)

In vitro binding studies of E2f and Dp to the DNApol-α180 promoter region reveals each of the E2f binding sites plays a distinct role as positive or negative element in the regulation of the DNApol-α180 promoter during development.

(Yamaguchi et al., 1997)

A specific polyclonal antibody stains interphase nuclei and the surrounding cytoplasmic regions. The protein is present in the embryo as a maternal product. The 130kD proteolytic product is also present in embryos as a maternal product; unlike the 180kD protein this decreases in amount after cycle 10 and is not detectable after cycle 14.

(Kuroda and Ueda, 1995)

Subunits of DNA polymerase α and δ are purified from embryos and characterised.

(Mitsis et al., 1995)

Expression pattern and subcellular distribution of the protein during development is studied using antibodies.

(Yamaguchi et al., 1995)

DNApol-α180 is negatively regulated by zen protein. This repression is mediated by the DRE (DNA replication-related element) sites in the DNApol-α180 promoter. The amount of Dref is reduced in transfected Kc cells expressing zen, suggesting that zen represses expression of DNA replication-related genes by reducing the amount of Dref.

(Hirose et al., 1994)

The properties of the DNA polymerase α enzyme have been studied.

(Cai et al., 1993)

DNApol-α and mus209 products share DRE, an 8bp palindrome required for high expression.

(Hirose et al., 1993)

Immunoaffinity-purified DNA polymerase α-primase includes not only the 180kD protein, but also 145, 140 and 130kD proteins, due to proteolytic cleavage of the 180kD protein at F130/S131, T180/S181 and F237/S238.

(Kuroda et al., 1993)

Embryonic DNA polymerase α complex, isolated by immunological techniques, contains a protein kinase activity. The kinase will phosphorylate His1, but prefers peptides contained in the DNA polymerase α kinase complex.

(Peck et al., 1993)

Mono Q anion exchange chromatography used to resolve DNA polymerase activities in 0-2.5hr embryos. DNApol-δ and DNApol-α distinguished by copurification with DNAprimase (DNApol-α) or 3'-5'exonuclease activity (DNApol-δ), immunoblot analysis with DNApol-α specific antibodies, sensitivity to aphidicolin (both DNApol-α and DNApol-δ sensitive) and BuPdGTP (DNApol-α more sensitive), and processivity measurements with and without PCNA (DNApol-δ has PCNA-inducible high processivity).

(Peck et al., 1992)

Recognition and binding of synthetic template-primers by DNA-polymerase-α holoenzyme studied: at least 4 base pair complementarity is required for efficient binding and incorporation. Results suggest that there are intrinsic aspects to the mechanism of nucleotide incorporation which ensure fidelity of DNA synthesis and may provide insight into mechanism of polymerase translocation along templates.

(Weiss and Fisher, 1992)

The nucleotide sequence of DNA polymerase α gene was determined. Expression of the enzyme is closely related to cell proliferation during development.

(Hirose et al., 1991)

25bp DNA oligomer containing m⁴T is used in a gel extension assay to measure the efficiency of incorporation of dATP and dGTP opposite m⁴T using a DNA polymerase α-primase complex. The DNA polymerase α shows a clear preference for pairing with dGTP.

(Dosanjh et al., 1990)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_080488

RefSeq (Proteins)

•

NP_536736

DDBJ / EMBL / GenBank

•

Entrez Gene - A searchable database of RefSeq genes.

•

42553

UniProtKB/Swiss-Prot

•

P26019

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

DNA-directed DNA polymerase, family B, conserved site (IPR017964)

DNA-directed DNA polymerase, family B, pol2 (IPR004578)

DNA-directed DNA polymerase, family B, exonuclease domain (IPR006133)

DNA-directed DNA polymerase, family B, multifunctional domain (IPR006134)

DNA-directed DNA polymerase, family B (IPR006172)

Ribonuclease H-like domain (IPR012337)

Zinc finger, DNA-directed DNA polymerase, family B, alpha (IPR015088)

DNA polymerase alpha catalytic subunit, N-terminal domain (IPR024647)

DNA polymerase, palm domain (IPR023211)

Linkouts

BioGRID - A database of protein and genetic interactions

•

67519

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0259113

DRSC - Results from RNAi screens.

•

FBgn0259113

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0259113

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0259113

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

42553

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

42553

modMine - Data generated by the modENCODE project.

•

FBgn0259113

REDfly - A database of transcriptional regulatory elements.

•

FBgn0259113

Synonyms & Secondary IDs ( 37 )

Reported As

Symbol Synonym

183 kDa pol α

(Peck et al., 1993)

AAB24152

(Burgers et al., 2001)

CG6349

(Dimova et al., 2003, Keleman et al., 2009.8.5, Suyari et al., 2012)

Dmpol α

(Takeuchi et al., 2004)

DmPolα

(Bosco et al., 2001)

DNApol

(Somma et al., 2008)

DNApola

(Maroni, 1994)

DNApol-alpha180

(Giot et al., 2003, Greil et al., 2003)

DNA polymerase α 180 kD

(Érdi et al., 2012)

DNA polα

(Frolov et al., 2003)

DNApol-α

(Hirose et al., 1996, Hirose et al., 1994, Shen et al., 1992, )

DNApolα

(Shinomiya and Ina, 1994, Cai et al., 1993)

DNA-Polα

(Kugler and Nagel, 2007)

DNApol-α180

(Cayirlioglu et al., 2003, LaRocque et al., 2007, )

DNA Pol-α180

(Kugler and Nagel, 2007)

DNApolα180

(Isogai et al., 2007)

E(mus304)

(LaRocque et al., 2007, )

Pol

(Machida et al., 2005)

POLA

(Sasaki, 1998.3.3, Hirose, 1991, Matsukage et al., 1995)

pol α

(Mizushina et al., 2003, Singer and Dosanjh, 1990)

polα

(Royzman and Orr-Weaver, 1998, Hyun et al., 2005)

Polα

(Lehman, 1998, Ullah et al., 2007)

Pol α

(Ullah et al., 2007)

POlα 180kDa

(Suyari et al., 2012)

polαp180

(Crevel et al., 2001)

Name Synonym

180 kDa subunit

(Melov et al., 1992)

180 kDa subunit of polymerase-primase

(Melov et al., 1992)

DNA pol 180

(Geng and MacDonald, 2007)

DNA polymerase 180K

(Davidson, 2003, Hochheimer et al., 2002)

DNA polymerase alpha

(Somma et al., 2008)

DNA polymerase-primase 180 kda subunit

(Melov, 1993.1.15)

DNA-polymerase α

(Leclerc et al., 1996)

DNA polymerase α

(Kamakaka et al., 1994, Marton et al., 1994, Mitsis et al., 1993, Matsukage et al., 1995, Kohzaki and Murakami, 2005, Hyun et al., 2005)

DNA polymerase α 148,000 α subunit

(Villani et al., 1980)

DNA polymerase α 180kD

DNA polymerase α 182,000 α subunit

(Kaguni et al., 1983)

Enhancer of mus304

(Brodsky et al., 2000)

Secondary FlyBase IDs

FBgn0004493
FBgn0010016
FBgn0041636

References ( 89 )

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All references relating to this gene ( 89 )

List References by type

Recent research papers ( 4 )

Lee et al., 2012, Mol. Cell. Biol. 32(11): 2110--2120: A Dual Role for the dREAM/MMB Complex in the Regulation of Differentiation-Specific E2F/RB Target Genes. [FBrf0218296]
Suyari et al., 2012, Gene 495(2): 104--114: Differential requirement for the N-terminal catalytic domain of the DNA polymerase ε p255 subunit in the mitotic cell cycle and the endocycle. [FBrf0217428]
Érdi et al., 2012, Autophagy 8(7): 1124--1135: Loss of the starvation-induced gene Rack1 leads to glycogen deficiency and impaired autophagic responses in Drosophila. [FBrf0219844]
Gosnell and Christensen, 2011, BMC Mol. Biol. 12: 13: Drosophila Ctf4 is essential for efficient DNA replication and normal cell cycle progression. [FBrf0213597]

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Recent reviews (0)

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