• Home
• Tools
  • Tools Overview
  • Query Tools

    
    • QuickSearch
    • QueryBuilder
    • TermLink
    • CytoSearch
    • Interactions Browser
    • ImageBrowse
    • Find A Person
    • Google™ FlyBase
  • Genomic/Map Tools

    
    • BLAST
    • GBrowse
    • CytoSearch
    • Aberrations Maps
    • Chromosome Maps
    • Coordinates Converter
  • Retrieve/Convert

    
    • Batch Download
    • Coordinates Converter
    • ID Converter
  • Data Submission

    
    • Fast-Track Your Paper
    • Submit Personal Comm
    • Add A New Person
    • Update Person Info
  • People

    
    • Find A Person
    • Add A New Person
    • Update Person Info
• Files
  • Files Overview
  • Downloads:
  • Precomputed files
  • Archived data
  • Releases (FTP)
  • Genomes (FTP)
  • AAA data (FTP)
  • Lists:
  • Transposons (Dmel)
  • Transposons (other)
  • Vectors & Constructs
  • Anatomical Terms
  • Map Conversion
• Species
  • Phylogeny
  • Sequenced Species
  • Synteny Table
  • Color Illustrations
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All Resources
  • Model Organisms
  • Stock Collections
  • Other links:
  • BDGP
  • DGRC
  • FlyExpress
  • Interactive Fly
  • modENCODE
  • Textpresso for Fly
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • FlyBase Guides
  • Video Tutorials
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Contact FlyBase
• Archives
  • Prior Release
  • Other Archives
  • Coordinate Converter

Gene Dmel\DNApol-α180

General Information
Symbol	Dmel\DNApol-α180	Species	D. melanogaster
Name	DNA polymerase α 180kD	Annotation symbol	CG6349
Feature type	protein_coding_gene	FlyBase ID	FBgn0259113
Gene Model Status	Current	Stock availability	4 publicly available
Also Known As	polα, pol α, POLA, DNApol-α, DNApol-alpha180, DNApolα
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	93F2-93F2	Sequence location	3R:17,492,275..17,497,472 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene DNA polymerase α 180kD is referred to in FlyBase by the symbol Dmel\DNApol-α180 (CG6349, FBgn0259113). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: DNA-directed DNA polymerase activity. There is experimental evidence that it is involved in the biological process: DNA replication; neurogenesis. 7 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: DNA polymerase alpha catalytic subunit, N-terminal domain; DNA polymerase, palm domain; DNA-directed DNA polymerase, family B; DNA-directed DNA polymerase, family B, conserved site; DNA-directed DNA polymerase, family B, exonuclease domain; DNA-directed DNA polymerase, family B, multifunctional domain; DNA-directed DNA polymerase, family B, pol2; Ribonuclease H-like; Zinc finger, DNA-directed DNA polymerase, family B, alpha. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1633249_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of DNApol-alpha180. Gene sequence location is 3R:17492275..17497472.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2012_01	Sequence features BKN20159 HFA16640
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 93F2-93F2   Limits computationally determined from genome sequence between P{PZ}InR05545 and P{PZ}tsl00617
Experimentally Determined Cytological Location
Cytogenetic map Notes References 93D-93F   Mapped by recombination and deficiency analysis.   (Brodsky et al., 2000) 93F1-93F1   (determined by in situ hybridisation)   (Melov et al., 1992)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\DNApol-α180 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	DGC clone appears problematic: incomplete CDS (GM13460)
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) DNApol-alpha180-RA FBtr0084115  NM_080488   4633   1488
Additional Transcript Data & Comments
Reported size (kB)	5.7 (northern blot) (Hirose et al., 1991) 4.8 (northern blot) (Melov et al., 1992)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein DNApol-alpha180-PA   FBpp0083514   169.9   1488   8.11     NP_536736   AAF55908
Additional Polypeptide Data & Comments
Reported size (kDa)	1505 (aa); 171 (kD) (Hirose et al., 1991) 1490 (aa); 180 (kD) (Melov et al., 1992) 183 (kD) (Peck et al., 1992)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • DNA-directed DNA polymerase, family B, conserved site (IPR017964) DNA-directed DNA polymerase, family B, pol2 (IPR004578) DNA-directed DNA polymerase, family B, exonuclease domain (IPR006133) DNA-directed DNA polymerase, family B, multifunctional domain (IPR006134) DNA-directed DNA polymerase, family B (IPR006172) Ribonuclease H-like (IPR012337) Zinc finger, DNA-directed DNA polymerase, family B, alpha (IPR015088) DNA polymerase alpha catalytic subunit, N-terminal domain (IPR024647) DNA polymerase, palm domain (IPR023211)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA263278   AA803549   AE014297 AAF55908   AB011813 BAA32745   AI518968   AY070529 AAL48000   BF499770   CO284757   CO322309   D90310 BAA14340   EC051377   EC052444   EC052448   EC052449   EC052992   EC054371   EC054890   EC071333   EC199831   EC211715   EC246466   EV576839   S48157 AAB24152
UniProtKB/Swiss-Prot	P26019
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References protein binding site FBsf0000160881 3R:17,497,594..17,497,626 bound_moiety=zen-XP evidence=experimental (Hirose et al., 1994, Bergman et al., 2004) protein binding site FBsf0000160882 3R:17,497,519..17,497,543 bound_moiety=Dref-XP evidence=experimental (Bergman et al., 2004, Hirose et al., 1993) protein binding site FBsf0000160883 3R:17,497,466..17,497,495 bound_moiety=Dref-XP evidence=experimental (Hirose et al., 1993, Bergman et al., 2004) protein binding site FBsf0000159814 3R:17,497,654..17,497,679 bound_moiety=Dref-XP evidence=experimental (Hirose et al., 1993, Bergman et al., 2004)
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
No Assay Recorded Stage Tissue/Position (including subcellular localization) Reference embryonic stage | early     (Melov et al., 1992) in situ Stage Tissue/Position (including subcellular localization) Reference oogenesis & adult stage | female   ovary   (Melov et al., 1992)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
No Assay Recorded Stage Tissue/Position (including subcellular localization) Reference first instar larval stage     (Melov et al., 1992) adult stage & oogenesis   follicle cell   (Melov et al., 1992) immunolocalization Stage Tissue/Position (including subcellular localization) Reference adult stage & oogenesis   nurse cell   • nucleus (Melov et al., 1992) western blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage | early     (Melov et al., 1992) pupal stage | early     (Melov et al., 1992)
Additional Descriptive Data
	DNApol-alpha180 protein is observed in nuclei of nurse cells and follicle cells. During early stages of embryogenesis, DNApol-alpha180 protein is mainly nuclear although dispersal occurrs during mitotic phases of the cell cycle. (Melov et al., 1992) DNApol-α180 activity is highest in embryos, 2nd instar larvae, and adult females. (Hirose et al., 1991)
Marker for
Subcellular Localization
CV Term	nucleus (Melov et al., 1992)
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0259113    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0259113 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0259113 expression level Developmental Stage   Expression Level embryo 00-02hr    2675 embryo 02-04hr    1939 embryo 04-06hr    4431 embryo 06-08hr    2089 embryo 08-10hr    1222 embryo 10-12hr    402 embryo 12-14hr    392 embryo 14-16hr    319 embryo 16-18hr    273 embryo 18-20hr    196 embryo 20-22hr    112 embryo 22-24hr    140 larva L1    408 larva L2    447 larva L3 12hr old    296 larva L3 puffstage 1-2    317 larva L3 puffstage 3-6    403 larva L3 puffstage 7-9    411 white prepupae new    373 white prepupae 12hr    602 white prepupae 24hr    378 pupae 2d postWPP    101 pupae 3d postWPP    63 pupae 4d postWPP    152 adult male 01day    62 adult male 05day    57 adult male 30day    54 adult female 01day    849 adult female 05day    1936 adult female 30day    1818 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (2675) embryo 02-04hr  (1939) embryo 04-06hr  (4431) embryo 06-08hr  (2089) embryo 08-10hr  (1222) embryo 10-12hr  (402) embryo 12-14hr  (392) embryo 14-16hr  (319) embryo 16-18hr  (273) embryo 18-20hr  (196) embryo 20-22hr  (112) embryo 22-24hr  (140) larva L1  (408) larva L2  (447) larva L3 12hr old  (296) larva L3 puffstage 1-2  (317) larva L3 puffstage 3-6  (403) larva L3 puffstage 7-9  (411) white prepupae new  (373) white prepupae 12hr  (602) white prepupae 24hr  (378) pupae 2d postWPP  101 pupae 3d postWPP    63 pupae 4d postWPP  (152) adult male 01day    62 adult male 05day    57 adult male 30day    54 adult female 01day  (849) adult female 05day  (1936) adult female 30day  (1818) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (2675) embryo 02-04hr  (1939) embryo 04-06hr  (4431) embryo 06-08hr  (2089) embryo 08-10hr    1222 embryo 10-12hr    402 embryo 12-14hr    392 embryo 14-16hr    319 embryo 16-18hr    273 embryo 18-20hr    196 embryo 20-22hr    112 embryo 22-24hr    140 larva L1    408 larva L2    447 larva L3 12hr old    296 larva L3 puffstage 1-2    317 larva L3 puffstage 3-6    403 larva L3 puffstage 7-9    411 white prepupae new    373 white prepupae 12hr    602 white prepupae 24hr    378 pupae 2d postWPP    101 pupae 3d postWPP    63 pupae 4d postWPP    152 adult male 01day    62 adult male 05day    57 adult male 30day    54 adult female 01day    849 adult female 05day  (1936) adult female 30day  (1818) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    2675 embryo 02-04hr    1939 embryo 04-06hr    4431 embryo 06-08hr    2089 embryo 08-10hr    1222 embryo 10-12hr    402 embryo 12-14hr    392 embryo 14-16hr    319 embryo 16-18hr    273 embryo 18-20hr    196 embryo 20-22hr    112 embryo 22-24hr    140 larva L1    408 larva L2    447 larva L3 12hr old    296 larva L3 puffstage 1-2    317 larva L3 puffstage 3-6    403 larva L3 puffstage 7-9    411 white prepupae new    373 white prepupae 12hr    602 white prepupae 24hr    378 pupae 2d postWPP    101 pupae 3d postWPP    63 pupae 4d postWPP    152 adult male 01day    62 adult male 05day    57 adult male 30day    54 adult female 01day    849 adult female 05day    1936 adult female 30day    1818 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    2675 embryo 02-04hr    1939 embryo 04-06hr    4431 embryo 06-08hr    2089 embryo 08-10hr    1222 embryo 10-12hr    402 embryo 12-14hr    392 embryo 14-16hr    319 embryo 16-18hr    273 embryo 18-20hr    196 embryo 20-22hr    112 embryo 22-24hr    140 larva L1    408 larva L2    447 larva L3 12hr old    296 larva L3 puffstage 1-2    317 larva L3 puffstage 3-6    403 larva L3 puffstage 7-9    411 white prepupae new    373 white prepupae 12hr    602 white prepupae 24hr    378 pupae 2d postWPP    101 pupae 3d postWPP    63 pupae 4d postWPP    152 adult male 01day    62 adult male 05day    57 adult male 30day    54 adult female 01day    849 adult female 05day    1936 adult female 30day    1818 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0259113 expression level Developmental Stage   Expression Level embryo 00-02hr    2675 embryo 02-04hr    1939 embryo 04-06hr    4431 embryo 06-08hr    2089 embryo 08-10hr    1222 embryo 10-12hr    402 embryo 12-14hr    392 embryo 14-16hr    319 embryo 16-18hr    273 embryo 18-20hr    196 embryo 20-22hr    112 embryo 22-24hr    140 larva L1    408 larva L2    447 larva L3 12hr old    296 larva L3 puffstage 1-2    317 larva L3 puffstage 3-6    403 larva L3 puffstage 7-9    411 white prepupae new    373 white prepupae 12hr    602 white prepupae 24hr    378 pupae 2d postWPP    101 pupae 3d postWPP    63 pupae 4d postWPP    152 adult male 01day    62 adult male 05day    57 adult male 30day    54 adult female 01day    849 adult female 05day    1936 adult female 30day    1818 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (2675) embryo 02-04hr  (1939) embryo 04-06hr  (4431) embryo 06-08hr  (2089) embryo 08-10hr  (1222) embryo 10-12hr  (402) embryo 12-14hr  (392) embryo 14-16hr  (319) embryo 16-18hr  (273) embryo 18-20hr  (196) embryo 20-22hr  112 embryo 22-24hr  140 larva L1  (408) larva L2  (447) larva L3 12hr old  (296) larva L3 puffstage 1-2  (317) larva L3 puffstage 3-6  (403) larva L3 puffstage 7-9  (411) white prepupae new  (373) white prepupae 12hr  (602) white prepupae 24hr  (378) pupae 2d postWPP  101 pupae 3d postWPP    63 pupae 4d postWPP  (152) adult male 01day    62 adult male 05day    57 adult male 30day    54 adult female 01day  (849) adult female 05day  (1936) adult female 30day  (1818) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (2675) embryo 02-04hr  1939 embryo 04-06hr  (4431) embryo 06-08hr  2089 embryo 08-10hr    1222 embryo 10-12hr    402 embryo 12-14hr    392 embryo 14-16hr    319 embryo 16-18hr    273 embryo 18-20hr    196 embryo 20-22hr    112 embryo 22-24hr    140 larva L1    408 larva L2    447 larva L3 12hr old    296 larva L3 puffstage 1-2    317 larva L3 puffstage 3-6    403 larva L3 puffstage 7-9    411 white prepupae new    373 white prepupae 12hr    602 white prepupae 24hr    378 pupae 2d postWPP    101 pupae 3d postWPP    63 pupae 4d postWPP    152 adult male 01day    62 adult male 05day    57 adult male 30day    54 adult female 01day    849 adult female 05day  1936 adult female 30day  1818 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    2675 embryo 02-04hr    1939 embryo 04-06hr    4431 embryo 06-08hr    2089 embryo 08-10hr    1222 embryo 10-12hr    402 embryo 12-14hr    392 embryo 14-16hr    319 embryo 16-18hr    273 embryo 18-20hr    196 embryo 20-22hr    112 embryo 22-24hr    140 larva L1    408 larva L2    447 larva L3 12hr old    296 larva L3 puffstage 1-2    317 larva L3 puffstage 3-6    403 larva L3 puffstage 7-9    411 white prepupae new    373 white prepupae 12hr    602 white prepupae 24hr    378 pupae 2d postWPP    101 pupae 3d postWPP    63 pupae 4d postWPP    152 adult male 01day    62 adult male 05day    57 adult male 30day    54 adult female 01day    849 adult female 05day    1936 adult female 30day    1818 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    2675 embryo 02-04hr    1939 embryo 04-06hr    4431 embryo 06-08hr    2089 embryo 08-10hr    1222 embryo 10-12hr    402 embryo 12-14hr    392 embryo 14-16hr    319 embryo 16-18hr    273 embryo 18-20hr    196 embryo 20-22hr    112 embryo 22-24hr    140 larva L1    408 larva L2    447 larva L3 12hr old    296 larva L3 puffstage 1-2    317 larva L3 puffstage 3-6    403 larva L3 puffstage 7-9    411 white prepupae new    373 white prepupae 12hr    602 white prepupae 24hr    378 pupae 2d postWPP    101 pupae 3d postWPP    63 pupae 4d postWPP    152 adult male 01day    62 adult male 05day    57 adult male 30day    54 adult female 01day    849 adult female 05day    1936 adult female 30day    1818 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0259113    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0259113 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0259113 expression level Tissue   Expression Level Larval Central Nervous System    112.6 Larval Midgut no informative data Larval Hindgut    44.4 Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland    88.5 Larval Trachea    48.85 Larval Carcass    53.475 Adult Head no informative data Adult Eye    3.575 Adult Brain    4.8 Adult Thoracic-Abdominal Ganglion    7.6 Adult Crop    7.4 Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules    6.7 Adult Fat Body no informative data Adult Salivary Gland    6 Adult Heart    2.55 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    8.2 Adult Ovary    218.8 Adult Testis no informative data Adult Male Accessory Gland    8.7 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    112.6 Larval Midgut no informative data Larval Hindgut    44.4 Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland    88.5 Larval Trachea    48.85 Larval Carcass    53.475 Adult Head no informative data Adult Eye    3.575 Adult Brain    4.8 Adult Thoracic-Abdominal Ganglion    7.6 Adult Crop    7.4 Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules    6.7 Adult Fat Body no informative data Adult Salivary Gland    6 Adult Heart    2.55 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    8.2 Adult Ovary    218.8 Adult Testis no informative data Adult Male Accessory Gland    8.7 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    112.6 Larval Midgut no informative data Larval Hindgut    44.4 Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland    88.5 Larval Trachea    48.85 Larval Carcass    53.475 Adult Head no informative data Adult Eye    3.575 Adult Brain    4.8 Adult Thoracic-Abdominal Ganglion    7.6 Adult Crop    7.4 Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules    6.7 Adult Fat Body no informative data Adult Salivary Gland    6 Adult Heart    2.55 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    8.2 Adult Ovary    218.8 Adult Testis no informative data Adult Male Accessory Gland    8.7 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    112.6 Larval Midgut no informative data Larval Hindgut    44.4 Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland    88.5 Larval Trachea    48.85 Larval Carcass    53.475 Adult Head no informative data Adult Eye    3.575 Adult Brain    4.8 Adult Thoracic-Abdominal Ganglion    7.6 Adult Crop    7.4 Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules    6.7 Adult Fat Body no informative data Adult Salivary Gland    6 Adult Heart    2.55 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    8.2 Adult Ovary    218.8 Adult Testis no informative data Adult Male Accessory Gland    8.7 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0259113 expression level Tissue   Expression Level Larval Central Nervous System    112.6 Larval Midgut no informative data Larval Hindgut    44.4 Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland    88.5 Larval Trachea    48.85 Larval Carcass    53.475 Adult Head no informative data Adult Eye    3.575 Adult Brain    4.8 Adult Thoracic-Abdominal Ganglion    7.6 Adult Crop    7.4 Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules    6.7 Adult Fat Body no informative data Adult Salivary Gland    6 Adult Heart    2.55 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    8.2 Adult Ovary    218.8 Adult Testis no informative data Adult Male Accessory Gland    8.7 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    112.6 Larval Midgut no informative data Larval Hindgut    44.4 Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland    88.5 Larval Trachea    48.85 Larval Carcass    53.475 Adult Head no informative data Adult Eye    3.575 Adult Brain    4.8 Adult Thoracic-Abdominal Ganglion    7.6 Adult Crop    7.4 Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules    6.7 Adult Fat Body no informative data Adult Salivary Gland    6 Adult Heart    2.55 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    8.2 Adult Ovary    218.8 Adult Testis no informative data Adult Male Accessory Gland    8.7 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    112.6 Larval Midgut no informative data Larval Hindgut    44.4 Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland    88.5 Larval Trachea    48.85 Larval Carcass    53.475 Adult Head no informative data Adult Eye    3.575 Adult Brain    4.8 Adult Thoracic-Abdominal Ganglion    7.6 Adult Crop    7.4 Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules    6.7 Adult Fat Body no informative data Adult Salivary Gland    6 Adult Heart    2.55 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    8.2 Adult Ovary    218.8 Adult Testis no informative data Adult Male Accessory Gland    8.7 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    112.6 Larval Midgut no informative data Larval Hindgut    44.4 Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland    88.5 Larval Trachea    48.85 Larval Carcass    53.475 Adult Head no informative data Adult Eye    3.575 Adult Brain    4.8 Adult Thoracic-Abdominal Ganglion    7.6 Adult Crop    7.4 Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules    6.7 Adult Fat Body no informative data Adult Salivary Gland    6 Adult Heart    2.55 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    8.2 Adult Ovary    218.8 Adult Testis no informative data Adult Male Accessory Gland    8.7 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut no informative data Larval Hindgut     Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head no informative data Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules     Adult Fat Body no informative data Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis no informative data Adult Male Accessory Gland     Adult Carcass no informative data FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    no informative data   NA  Eye    3.575   NA  Brain    4.8   112.6  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    7.6   NA  Crop    7.4   no informative data  Midgut    no informative data   44.4  Hindgut    no informative data   no informative data  Malpighian Tubules    6.7   no informative data  Fat Body    no informative data   88.5  Salivary Gland    6   NA  Heart    2.55   48.85  Trachea    NA   NA  VirginFemale Spermatheca    no informative data   NA  InseminatedFemale Spermatheca    8.2   NA  Ovary    218.8   NA  Testis    no informative data   NA  Male Accessory Gland    8.7   53.475  Carcass    no informative data modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_13
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0259113
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL41407 DNApol-α180GD4238 lethal (with DNApol-α180Emus304) DNApol-α180Q1132ter DNApol-α180Q1322ter lethal (with DNApol-α180Q1132ter) DNApol-α180Emus304 lethal (with DNApol-α180Q1322ter) DNApol-α180Emus304 lethal | embryonic stage | recessive DNApol-α180Emus304 lethal | pupal stage, with Scer\GAL4pnr-MD237 DNApol-α180GD4238 lethal | recessive DNApol-α180Emus304 Other Phenotypes Allele neuroanatomy defective | larval stage, with Scer\GAL41407 DNApol-α180GD4238 Phenotype manifest in Allele embryonic/larval neuroblast | larval stage, with Scer\GAL41407 DNApol-α180GD4238 ganglion mother cell | larval stage, with Scer\GAL41407 DNApol-α180GD4238
Classical Alleles ( 5 )
For All Classical Alleles Show
Allele of DNApol-α180 Class Mutagen Stocks Known lesion DNApol-α180G13925 P-element activity 1 -- DNApol-α180NP0280 1 -- DNApol-α180Emus304 spontaneous 0 Yes DNApol-α180Q1132ter ethyl methanesulfonate 0 Yes DNApol-α180Q1322ter ethyl methanesulfonate 0 Yes
Alleles Carried on Transgenic Constructs ( 2 )
For All Alleles Carried on Transgenic Constructs Show
Allele of DNApol-α180 Class Mutagen Stocks Known lesion DNApol-α180GD4238 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes DNApol-α180KK100063 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)Exel6186 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data reporter construct P{5'-358DpolαlacZ} No P{5'-358DpolαlacZmut1} No P{5'-358DpolαlacZmut1+2+3} No P{5'-358DpolαlacZmut2} No P{5'-358DpolαlacZmut2+3} No P{5'-358DpolαlacZmut3} No
Insertions
	Type of insertions Name Expression data insertion of enhancer trap binary system P{GawB}DNApol-α180NP0280 No insertion of mobile activating element P{EP}DNApol-α180G13925 NA
Gene Ontology: Function, Process & Cellular Component ( 10 unique terms )
Terms Based on Experimental Evidence ( 5 terms )
Molecular Function
CV term Evidence References
DNA-directed DNA polymerase activity inferred from direct assay (Peck et al., 1992, Kaguni et al., 1983, Villani et al., 1980)
Biological Process
CV term Evidence References
DNA replication inferred from direct assay (Kaguni et al., 1983, Villani et al., 1980) neurogenesis inferred from mutant phenotype (Neumüller et al., 2011)
Cellular Component
CV term Evidence References
alpha DNA polymerase:primase complex inferred from direct assay (Peck et al., 1992, Kaguni et al., 1983, Villani et al., 1980) nucleus inferred from direct assay (Melov et al., 1992)
Terms Based on Predictions or Assertions ( 7 terms )
Molecular Function
CV term Evidence References
3'-5'-exodeoxyribonuclease activity non-traceable author statement (Swiss-Prot Project Members, 1992.5.1) DNA binding inferred from electronic annotation with InterPro:IPR004578, InterPro:IPR006134 (FlyBase Curators et al., 2004-) DNA-directed DNA polymerase activity inferred from sequence or structural similarity with UniProtKB:P09884 (FlyBase, 1992-) non-traceable author statement (Burgers et al., 2001, Swiss-Prot Project Members, 1992.5.1) nucleoside binding inferred from electronic annotation with InterPro:IPR015088 (FlyBase Curators et al., 2004-) nucleotide binding inferred from electronic annotation with InterPro:IPR004578, InterPro:IPR006134, InterPro:IPR006172, InterPro:IPR017964 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
DNA strand elongation involved in DNA replication non-traceable author statement (Swiss-Prot Project Members, 1992.5.1)
Cellular Component
CV term Evidence References
alpha DNA polymerase:primase complex inferred from sequence or structural similarity with UniProtKB:P09884 (FlyBase, 1992-) non-traceable author statement (Swiss-Prot Project Members, 1992.5.1)
Sequence Ontology: Class of Gene
	gene   nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions DNApol-α180 allele Gene References E(mus304)1 mei-41 (Brodsky et al., 2000) mus304 (Brodsky et al., 2000) unspecified mei-41 (LaRocque et al., 2007)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 67519 DroID - A comprehensive database of gene and protein interactions. • FBgn0259113 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 42553
Orthologs
Genome-wide drosophilid orthologs	Dana\GF17808 Dere\GG14396 Dgri\GH17298 Dmoj\GI10263 Dper\GL21505 Dpse\GA19530 Dsec\GM10998 Dsim\GD19968 Dvir\GJ11070 Dwil\GK12074 Dyak\GE24967
Curated drosophilid orthologs	Dpse\GA19530
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL008247-PA Aedes aegypti (mosquito) AGAP001929-PA Anopheles gambiae (mosquito) XP_001121438.1 Apis mellifera (honey bee) At5g67100.1 Arabidopsis thaliana (wall cress) 85131 Batrachochytrium dendrobatidis (chytrid fungus) 120187 Branchiostoma floridae (lancelet) BGIBMGA003396-PA Bombyx mori (silk worm) ENSBTAP00000010742 Bos taurus (bovine) CAL0002431 Candida albicans (a form of yeast) CBP37206 Caenorhabditis briggsae (nematode) CE32262 Caenorhabditis elegans (worm) ENSCAFP00000019931 Canis familiaris (dog) CAGL0G06666g Candida glabrata (haploid yeast) ENSCINP00000003220 Ciona intestinalis (sea squirt) JA15097 Caenorhabditis japonica (nematode) CMI176C Cyanidioschyzon merolae (red alga) cn06073 Cryptococcus neoformans (yeast-like fungus) cgd3_4290 Cryptosporidium parvum (protozoan parasite) CPIJ015260 Culex pipens (mosquito) ENSCPOP00000001937 Cavia porcellus (guinea pig) 177262 Chlamidomonas reinhardtii (green alga) RP28251 Caenorhabditis remanei (nematode) ENSCSAVP00000001303 Ciona savignyi (sedentary tunicate) 228337 Capitella spl (polychaete worm) DDB0232274 Dictyostelium discoideum (amoeba) DEHA2F11440g Debaryomyces Hansenii (yeast) 304346 Daphnia pulex (daphnia) ENSDARP00000066648 Danio rerio (zebra fish) ENSECAP00000011308 Equus caballus (horse) NP_414602.1 Escherichia coli K12 (bacteria) XP_657373 Entamoeba histolytica (anaerobic protozoan) ENSGACP00000003398 Gymnopilus aculeatus (mushroom) ENSGALP00000026266 Gallus gallus (chicken) GL50803_27326 Giardia lamblia (flagellated protozoan) 193403 Hypsipyla robusta (cedar shoot borer) ENSP00000368358 Homo sapiens (human) ISCW012960-PA Ixodes scapularis (deer tick) KLLA0C13255g Kluyveromyces lactis (yeast) 109334 Lottia gigantea (owl limpet) LmjF16.1540 Leishmania major (virus) 107 Monocosta brevicollis (choanoflagellate) ENSMODP00000009125 Monodelphis domestica (gray opossum) ENSMMUP00000024252 Macaca mulatta (rhesus monkey) ENSMUSP00000006856 Mus musculus (house mouse) 168297 Nematostella vectensis (starlet anemone) XP_001603716.1 Nasonia vitripennis (wasp) ENSORLP00000009391 Oryzias latipes (japanese killifish) LOC_Os01g64820.1 Oryza sativa (asian rice) 18655 Ostreococcus tauri (green alga) PFD0590c Plasmodium falciparum (protozoal parasite) PHUM004232-PA Pediculus humanus (human body louse) PP00070 Pristionchus pacificus (nematode) 60547 Physcomitrella patens (moss) ENSPPYP00000022602 Pongo pygmaeus (orangutan) 74103 Phytophthora ramorum (sudden oak death pathogen) 132002 Phytophthora sojae (root rot pathogen) 218639 Populus trichocarpa (black cottonwood) ENSPTRP00000037302 Pan troglodytes (chimpanzee) PVX_000650 Plasmodium vivax (protozoal parasite) ENSRNOP00000018147 Rattus norvegicus (norway rat) 5054089 Sorghum bicolor (sorghum) YNL102W Saccharomyces cerevisiae (budding yeast) Smp_178260 Schistosoma mansoni (trematode parasite) SPAC3H5.06c Schizosaccharomyces pombe (fission yeast) 49541 Trichoplax adhaerens (metazoa) TA03330 Theileria annulata (apicomplexan parasite) XP_967510.2 Tribolium castaneum (red flour beetle) Tc00.1047053508837.180 Trypanosoma cruzi (parasitic trypanosome) ENSTNIP00000010984 Tetraodon nigroviridis (green spotted pufferfish) TP03_0202 Theileria parva (parasitic protozoan) ENSTRUP00000019792 Takifugu rubripes (pufferfish Takifugu) 47.m00199 Tetrahymena thermophila (ciliate protozoa) 86827.m00416 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000047196 Xenopus tropicalis (frog) YALI0E11869g Yarrowia lipolytica (nonconventional yeast) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB17252 NV17703 NV16702 LH23420 ISCW012960 FBgn0242061 FBgn0214085 FBgn0198331 FBgn0191451 FBgn0165943 JGI_V11_304346 FBgn0079526 FBgn0159098 FBgn0133028 FBgn0124768 FBgn0106655 FBgn0094826 CPIJ015261 CPIJ015260 BGIBMGA003396 GB18821 AGAP001929 AAEL008247 PHUM210770 GLEAN_13712
Stocks & Reagents
Stocks Listed in FlyBase ( 4 )
Bloomington	31805 w1118; P{EP}DNApol-α180G13925/TM6C, Sb1
Kyoto	103575 w*; P{GawB}DNApol-α180NP0280 / TM6C, Sb1
VDRC	v103699 P{KK100063}VIE-260B v11227 w1118 P{GD4238}v11227
Genomic Clones ( 1 )
	BACR16N15 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 17 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GM13460
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT14505 LD06369
Other clones	52091 59988 62548 CK01.129C.C11 Dmel_dig_008146_0649_1499 EC051377 EC052444 EC052448 EC052449 EC052992 EC054371 EC054890 EK166723 EK187851
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0259113 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 42553 250081
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: DNApol-α180 E(mus304) (LaRocque et al., 2007)
Additional comments
Other Comments
	Reducing DNApol-α180 levels by itself des not result in any defects, while reducing DNApol-α180 levels in the absence of mei-41 results in elevated p53-dependent apoptosis, rough eyes and increased genomic instability. (LaRocque et al., 2007) In vitro binding studies of E2f and Dp to the DNApol-α180 promoter region reveals each of the E2f binding sites plays a distinct role as positive or negative element in the regulation of the DNApol-α180 promoter during development. (Yamaguchi et al., 1997) A specific polyclonal antibody stains interphase nuclei and the surrounding cytoplasmic regions. The protein is present in the embryo as a maternal product. The 130kD proteolytic product is also present in embryos as a maternal product; unlike the 180kD protein this decreases in amount after cycle 10 and is not detectable after cycle 14. (Kuroda and Ueda, 1995) Subunits of DNA polymerase α and δ are purified from embryos and characterised. (Mitsis et al., 1995) Expression pattern and subcellular distribution of the protein during development is studied using antibodies. (Yamaguchi et al., 1995) DNApol-α180 is negatively regulated by zen protein. This repression is mediated by the DRE (DNA replication-related element) sites in the DNApol-α180 promoter. The amount of Dref is reduced in transfected Kc cells expressing zen, suggesting that zen represses expression of DNA replication-related genes by reducing the amount of Dref. (Hirose et al., 1994) The properties of the DNA polymerase α enzyme have been studied. (Cai et al., 1993) DNApol-α and mus209 products share DRE, an 8bp palindrome required for high expression. (Hirose et al., 1993) Immunoaffinity-purified DNA polymerase α-primase includes not only the 180kD protein, but also 145, 140 and 130kD proteins, due to proteolytic cleavage of the 180kD protein at F130/S131, T180/S181 and F237/S238. (Kuroda et al., 1993) Embryonic DNA polymerase α complex, isolated by immunological techniques, contains a protein kinase activity. The kinase will phosphorylate His1, but prefers peptides contained in the DNA polymerase α kinase complex. (Peck et al., 1993) Mono Q anion exchange chromatography used to resolve DNA polymerase activities in 0-2.5hr embryos. DNApol-δ and DNApol-α distinguished by copurification with DNAprimase (DNApol-α) or 3'-5'exonuclease activity (DNApol-δ), immunoblot analysis with DNApol-α specific antibodies, sensitivity to aphidicolin (both DNApol-α and DNApol-δ sensitive) and BuPdGTP (DNApol-α more sensitive), and processivity measurements with and without PCNA (DNApol-δ has PCNA-inducible high processivity). (Peck et al., 1992) Recognition and binding of synthetic template-primers by DNA-polymerase-α holoenzyme studied: at least 4 base pair complementarity is required for efficient binding and incorporation. Results suggest that there are intrinsic aspects to the mechanism of nucleotide incorporation which ensure fidelity of DNA synthesis and may provide insight into mechanism of polymerase translocation along templates. (Weiss and Fisher, 1992) The nucleotide sequence of DNA polymerase α gene was determined. Expression of the enzyme is closely related to cell proliferation during development. (Hirose et al., 1991) 25bp DNA oligomer containing m4T is used in a gel extension assay to measure the efficiency of incorporation of dATP and dGTP opposite m4T using a DNA polymerase α-primase complex. The DNA polymerase α shows a clear preference for pairing with dGTP. (Dosanjh et al., 1990)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_080488 RefSeq (Proteins) • NP_536736 DDBJ / EMBL / GenBank • AA803549 AAB24152 AAF55908 AAL48000 AB011813 AI518968 AY070529 BAA14340 BAA32745 D90310 S48157 Entrez Gene - A searchable database of RefSeq genes. • 42553 UniProtKB/Swiss-Prot • P26019
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • DNA-directed DNA polymerase, family B, conserved site (IPR017964) DNA-directed DNA polymerase, family B, pol2 (IPR004578) DNA-directed DNA polymerase, family B, exonuclease domain (IPR006133) DNA-directed DNA polymerase, family B, multifunctional domain (IPR006134) DNA-directed DNA polymerase, family B (IPR006172) Ribonuclease H-like (IPR012337) Zinc finger, DNA-directed DNA polymerase, family B, alpha (IPR015088) DNA polymerase alpha catalytic subunit, N-terminal domain (IPR024647) DNA polymerase, palm domain (IPR023211)
Linkouts
	BioGRID - A database of protein and genetic interactions • 67519 DroID - A comprehensive database of gene and protein interactions. • FBgn0259113 DRSC - Results from RNAi screens. • FBgn0259113 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0259113 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0259113 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 42553 250081 InParanoid A subset of ortholog calls from InParanoid. • AAEL008247-PA Aedes aegypti (mosquito) AGAP001929-PA Anopheles gambiae (mosquito) XP_001121438.1 Apis mellifera (honey bee) At5g67100.1 Arabidopsis thaliana (wall cress) 85131 Batrachochytrium dendrobatidis (chytrid fungus) 120187 Branchiostoma floridae (lancelet) BGIBMGA003396-PA Bombyx mori (silk worm) ENSBTAP00000010742 Bos taurus (bovine) CAL0002431 Candida albicans (a form of yeast) CBP37206 Caenorhabditis briggsae (nematode) CE32262 Caenorhabditis elegans (worm) ENSCAFP00000019931 Canis familiaris (dog) CAGL0G06666g Candida glabrata (haploid yeast) ENSCINP00000003220 Ciona intestinalis (sea squirt) JA15097 Caenorhabditis japonica (nematode) CMI176C Cyanidioschyzon merolae (red alga) cn06073 Cryptococcus neoformans (yeast-like fungus) cgd3_4290 Cryptosporidium parvum (protozoan parasite) CPIJ015260 Culex pipens (mosquito) ENSCPOP00000001937 Cavia porcellus (guinea pig) 177262 Chlamidomonas reinhardtii (green alga) RP28251 Caenorhabditis remanei (nematode) ENSCSAVP00000001303 Ciona savignyi (sedentary tunicate) 228337 Capitella spl (polychaete worm) DDB0232274 Dictyostelium discoideum (amoeba) DEHA2F11440g Debaryomyces Hansenii (yeast) 304346 Daphnia pulex (daphnia) ENSDARP00000066648 Danio rerio (zebra fish) ENSECAP00000011308 Equus caballus (horse) NP_414602.1 Escherichia coli K12 (bacteria) XP_657373 Entamoeba histolytica (anaerobic protozoan) ENSGACP00000003398 Gymnopilus aculeatus (mushroom) ENSGALP00000026266 Gallus gallus (chicken) GL50803_27326 Giardia lamblia (flagellated protozoan) 193403 Hypsipyla robusta (cedar shoot borer) ENSP00000368358 Homo sapiens (human) ISCW012960-PA Ixodes scapularis (deer tick) KLLA0C13255g Kluyveromyces lactis (yeast) 109334 Lottia gigantea (owl limpet) LmjF16.1540 Leishmania major (virus) 107 Monocosta brevicollis (choanoflagellate) ENSMODP00000009125 Monodelphis domestica (gray opossum) ENSMMUP00000024252 Macaca mulatta (rhesus monkey) ENSMUSP00000006856 Mus musculus (house mouse) 168297 Nematostella vectensis (starlet anemone) XP_001603716.1 Nasonia vitripennis (wasp) ENSORLP00000009391 Oryzias latipes (japanese killifish) LOC_Os01g64820.1 Oryza sativa (asian rice) 18655 Ostreococcus tauri (green alga) PFD0590c Plasmodium falciparum (protozoal parasite) PHUM004232-PA Pediculus humanus (human body louse) PP00070 Pristionchus pacificus (nematode) 60547 Physcomitrella patens (moss) ENSPPYP00000022602 Pongo pygmaeus (orangutan) 74103 Phytophthora ramorum (sudden oak death pathogen) 132002 Phytophthora sojae (root rot pathogen) 218639 Populus trichocarpa (black cottonwood) ENSPTRP00000037302 Pan troglodytes (chimpanzee) PVX_000650 Plasmodium vivax (protozoal parasite) ENSRNOP00000018147 Rattus norvegicus (norway rat) 5054089 Sorghum bicolor (sorghum) YNL102W Saccharomyces cerevisiae (budding yeast) Smp_178260 Schistosoma mansoni (trematode parasite) SPAC3H5.06c Schizosaccharomyces pombe (fission yeast) 49541 Trichoplax adhaerens (metazoa) TA03330 Theileria annulata (apicomplexan parasite) XP_967510.2 Tribolium castaneum (red flour beetle) Tc00.1047053508837.180 Trypanosoma cruzi (parasitic trypanosome) ENSTNIP00000010984 Tetraodon nigroviridis (green spotted pufferfish) TP03_0202 Theileria parva (parasitic protozoan) ENSTRUP00000019792 Takifugu rubripes (pufferfish Takifugu) 47.m00199 Tetrahymena thermophila (ciliate protozoa) 86827.m00416 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000047196 Xenopus tropicalis (frog) YALI0E11869g Yarrowia lipolytica (nonconventional yeast) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 42553 modMine - Data generated by the modENCODE project. • FBgn0259113 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB17252 NV17703 NV16702 LH23420 ISCW012960 FBgn0242061 FBgn0214085 FBgn0198331 FBgn0191451 FBgn0165943 JGI_V11_304346 FBgn0079526 FBgn0159098 FBgn0133028 FBgn0124768 FBgn0106655 FBgn0094826 CPIJ015261 CPIJ015260 BGIBMGA003396 GB18821 AGAP001929 AAEL008247 PHUM210770 GLEAN_13712 REDfly - A database of transcriptional regulatory elements. • FBgn0259113
Synonyms & Secondary IDs ( 35 )
Reported As
Symbol Synonym	183 kDa pol α (Peck et al., 1993) AAB24152 (Burgers et al., 2001) CG6349 (Dimova et al., 2003, Keleman et al., 2009.8.5) Dmpol α (Takeuchi et al., 2004) DmPolα (Bosco et al., 2001) DNApol (Somma et al., 2008) DNApola (Maroni, 1994) DNApol-alpha180 (Giot et al., 2003, Greil et al., 2003) DNA polα (Frolov et al., 2003) DNApol-α (Hirose et al., 1996, Hirose et al., 1994, Shen et al., 1992, ) DNApolα (Shinomiya and Ina, 1994, Cai et al., 1993) DNA-Polα (Kugler and Nagel, 2007) DNApol-α180 (Cayirlioglu et al., 2003, LaRocque et al., 2007, ) DNA Pol-α180 (Kugler and Nagel, 2007) DNApolα180 (Isogai et al., 2007) E(mus304) (LaRocque et al., 2007, ) Pol (Machida et al., 2005) POLA (Sasaki, 1998.3.3, Hirose, 1991, Matsukage et al., 1995) pol α (Mizushina et al., 2003, Singer and Dosanjh, 1990) polα (Royzman and Orr-Weaver, 1998, Hyun et al., 2005) Polα (Lehman, 1998, Ullah et al., 2007) Pol α (Ullah et al., 2007) polαp180 (Crevel et al., 2001)
Name Synonym	180 kDa subunit (Melov et al., 1992) 180 kDa subunit of polymerase-primase (Melov et al., 1992) DNA pol 180 (Geng and MacDonald, 2007) DNA polymerase 180K (Davidson, 2003, Hochheimer et al., 2002) DNA polymerase alpha (Somma et al., 2008) DNA polymerase-primase 180 kda subunit (Melov, 1993.1.15) DNA-polymerase α (Leclerc et al., 1996) DNA polymerase α (Kamakaka et al., 1994, Marton et al., 1994, Mitsis et al., 1993, Matsukage et al., 1995, Kohzaki and Murakami, 2005, Hyun et al., 2005) DNA polymerase α 148,000 α subunit (Villani et al., 1980) DNA polymerase α 180kD   DNA polymerase α 182,000 α subunit (Kaguni et al., 1983) Enhancer of mus304 (Brodsky et al., 2000)
Secondary FlyBase IDs
	FBgn0004493 FBgn0010016 FBgn0041636
References ( 83 )
Generate a list of	All references relating to this gene ( 83 )
List References by type	Research paper  ( 55 ) Supplementary material  ( 4 ) Review  ( 8 ) Personal communication to FlyBase  ( 5 ) Abstract  ( 2 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 3 ) Protein sequence record  ( 1 ) Computer file  ( 1 ) Automatic genome annotation  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 1 )
	Gosnell and Christensen, 2011, BMC Mol. Biol. 12: 13 Drosophila Ctf4 is essential for efficient DNA replication and normal cell cycle progression. [FBrf0213597] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...