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Gene Dmel\mnb

General Information
Symbol	Dmel\mnb	Species	D. melanogaster
Name	minibrain	Annotation symbol	CG42273
Feature type	protein_coding_gene	FlyBase ID	FBgn0259168
Gene Model Status	Current	Stock availability	17 publicly available
Also Known As	Dyrk1, CG7826, ME-IV
Genomic Location
Chromosome (arm)	X	Recombination map	1-58.2
Cytogenetic map	16E4-16F1	Sequence location	X:17,756,718..17,781,240 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene minibrain is referred to in FlyBase by the symbol Dmel\mnb (CG42273, FBgn0259168). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: protein serine/threonine/tyrosine kinase activity; protein serine/threonine kinase activity. There is experimental evidence that it is involved in the biological process: olfactory learning; circadian rhythm; nervous system development; visual behavior; brain development. 25 alleles are reported. The phenotypes of these alleles are annotated with: trichogen cell; embryonic/larval brain. It has 4 annotated transcripts and 4 annotated polypeptides. Protein features are: Protein kinase, ATP binding site; Protein kinase, catalytic domain; Protein kinase-like domain; Serine/threonine-protein kinase, active site; Serine/threonine-protein kinase, catalytic domain; Serine/threonine-protein kinase-like domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed at stages throughout embryogenesis, during late larval stages, during early pupal stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. Gene sequence location is X:17756718..17781240.
External Summaries
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	mnb: minibrain (J.C. Hall) Brain mass approximately half normal, including smaller than normal optic lobes and reduction in cell number (Fischbach and Heisenberg, 1984; Heisenberg and Wolf, 1984); exceptions: neuropile of the lamina optic ganglion appears normal in size and general morphology, and peduncle of mushroom body has apparently normal number of fibers; mnb flies take abnormally long to eclose (i.e., emergence per se from the pupal case is a slow process); behaviorally, mnb leads to relatively subtle behavioral defects, such as mild leg shaking under ether (see "other information"), absence of learning in tests using olfactory stimuli (Heisenberg et al., 1985), and aberrant visual fixation. In tests of locomotor activity rhythms (Helfrich, 1986), singly mutant mnb adults are basically normal, but a high proportion of mnb so double mutants show complex rhymicities (dual circadian periodicities) with about 20% being arrhythmic (3 times greater than wild type). Other behaviors, such as basic optomotor responses (M. Heisenberg, unpublished) and male courtship song (Kulkarni and Hall, 1987, Genetics 115: 461-475), are normal; physiologically, mnb--when linked to reduced optic lobes and small optic lobes mutations--has been tested for effects on electroretinogram, which have diminished amplitudes of light-on and light-off transient spikes in the triple mutant (Coombe, 1986, J. Comp. Physiol. 159: 655-665).
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01	Sequence features BKN50998 BKN22708 HFA20353 HFA20058 BKN50330 BKN46408 BKN33163
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 16E4-16F1   Limits computationally determined from genome sequence between P{EP}EP1464 and P{EP}EP970
Experimentally Determined Cytological Location
Cytogenetic map Notes References 16F-16F     (Pongs, 1993.1.22)
Experimentally Determined Recombination Data
Location	1-58.2
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\mnb for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene merge based on EST/cDNA data Due to an oversight in the implementation of gene annotation merges, the symbols for some of the transcripts and polypeptides of this gene were changed to non-unique designations in r5.8. Although there were no conflicts within a given release, the same symbols were used for different isoforms in different releases. The affected gene product symbols have now been changed to symbols not used previously in the database; see individual transcript reports for details.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) mnb-RE FBtr0300508  NM_167581   5504   908 mnb-RF FBtr0299619  NM_170658   4445   607 mnb-RG FBtr0300509  NM_001014750   4242   604 mnb-RH FBtr0300510  NM_170659   4228   1047
Additional Transcript Data & Comments
Reported size (kB)	5.5, 4.4, 4.2 (northern blot) (Tejedor et al., 1995)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein mnb-PE   FBpp0289735   95.9   908   9.11     NP_728104   AAF48777 mnb-PF   FBpp0288894   66.3   607   9.49     NP_728106   AAN09442 mnb-PG   FBpp0289736   65.9   604   9.28     NP_001014750   AAX52504 mnb-PH   FBpp0289737   111.1   1047   8.45     NP_728107   AAF48778
Additional Polypeptide Data & Comments
Reported size (kDa)	843, 542, 539 (aa); 90, 60, 58 (kD observed); 89, 59.4, 59.1 (kD predicted) (Tejedor et al., 1995)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, catalytic domain (IPR000719) Serine/threonine-protein kinase, catalytic domain (IPR002290) Serine/threonine-protein kinase, active site (IPR008271) Protein kinase-like domain (IPR011009) Protein kinase, ATP binding site (IPR017441) Serine/threonine-protein kinase-like domain (IPR017442)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA390386   AA440036   AA539656   AA802098   AE014298 AAF48777   AE014298 AAF48778   AE014298 AAN09442   AE014298 AAX52504   AI061748   AI260684   AY061418 AAL28966   BG635832   BI242285   BI358647   BI616088   BI623564   BP542354   BP548611   BT015255 AAT94484   CK132450   CO179088   CO185494   CO262769   CO265449   CO266087   CO270763   CO274569   CO275322   CO275826   CO279318   CO282144   CO286397   CO288655   CO291737   CO292963   CO295148   CO300468   CO302960   CO304821   CO324535   CO329877   CO331794   CO339620   CO340898   CO343423   CO345554   CW848256   CZ477233   CZ477234   CZ477718   CZ477719   CZ480244   CZ481640   CZ481641   CZ483846   CZ487019   CZ487020   EC052179   EC052689   EC053180   EC054185   EC056730   EC061268   EC070023   EC079618   EC083269   EC086467   EC090259   EC196230   EC196721   EC199168   EC199954   EC205876   EC208072   EC210362   EC212164   EC212968   EC216536   EC218681   EC219050   EC220307   EC220958   EC227337   EC228407   EC228712   EC234861   EC235652   EC237727   EC240328   EC245417   EC257098   EC258662   X70794 CAA50065   X70798 CAA50068   X70799 CAA50069
UniProtKB/Swiss-Prot	P49657
UniProtKB/TrEMBL	Q9VX06
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage   ventral nerve cord   (Tejedor et al., 1995) supraesophageal ganglion   (Tejedor et al., 1995)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference larval stage   embryonic/larval optic lobe   (Tejedor et al., 1995) mushroom body   (Tejedor et al., 1995) larval outer optic anlage   (Tejedor et al., 1995) adult stage   pigment cell   (Tejedor et al., 1995) mushroom body gamma-lobe   (Tejedor et al., 1995) retina   (Tejedor et al., 1995) mushroom body   (Tejedor et al., 1995) mushroom body beta-lobe   (Tejedor et al., 1995) western blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage -- adult stage     (Tejedor et al., 1995) larval stage & pupal stage     (Tejedor et al., 1995)
Additional Descriptive Data
	Higher levels of the 843aa form of mnb protein are observed in embryos and pupae than larvae. (Tejedor et al., 1995) Higher levels of the 542aa form of mnb protein are observed in embryos and pupae than larvae. (Tejedor et al., 1995) The 539aa form of mnb is expressed most strongly in larvae and pupae and is the predominant form in third instar larvae. (Tejedor et al., 1995) mnb protein is most prominent in the mushroom body neuropil and the outer proliferation centers of the optic lobes in the larval brain. In adult brains, the level of mnb protein is low in the optic lobes and central brain hemispheres but is high in retinal pigment cells as well as in the α, β, and γ cells and the peduncle of the mushroom bodies. (Tejedor et al., 1995)
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0259168    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0259168 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed at stages throughout embryogenesis, during late larval stages, during early pupal stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0259168 expression level Developmental Stage   Expression Level embryo 00-02hr    3549 embryo 02-04hr    3526 embryo 04-06hr    2382 embryo 06-08hr    2030 embryo 08-10hr    1974 embryo 10-12hr    1372 embryo 12-14hr    1334 embryo 14-16hr    1413 embryo 16-18hr    1148 embryo 18-20hr    706 embryo 20-22hr    1495 embryo 22-24hr    1207 larva L1    438 larva L2    380 larva L3 12hr old    289 larva L3 puffstage 1-2    336 larva L3 puffstage 3-6    1143 larva L3 puffstage 7-9    2383 white prepupae new    2796 white prepupae 12hr    1817 white prepupae 24hr    1209 pupae 2d postWPP    1155 pupae 3d postWPP    738 pupae 4d postWPP    839 adult male 01day    730 adult male 05day    578 adult male 30day    500 adult female 01day    1350 adult female 05day    1938 adult female 30day    1986 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (3549) embryo 02-04hr  (3526) embryo 04-06hr  (2382) embryo 06-08hr  (2030) embryo 08-10hr  (1974) embryo 10-12hr  (1372) embryo 12-14hr  (1334) embryo 14-16hr  (1413) embryo 16-18hr  (1148) embryo 18-20hr  (706) embryo 20-22hr  (1495) embryo 22-24hr  (1207) larva L1  (438) larva L2  (380) larva L3 12hr old  (289) larva L3 puffstage 1-2  (336) larva L3 puffstage 3-6  (1143) larva L3 puffstage 7-9  (2383) white prepupae new  (2796) white prepupae 12hr  (1817) white prepupae 24hr  (1209) pupae 2d postWPP  (1155) pupae 3d postWPP  (738) pupae 4d postWPP  (839) adult male 01day  (730) adult male 05day  (578) adult male 30day  (500) adult female 01day  (1350) adult female 05day  (1938) adult female 30day  (1986) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (3549) embryo 02-04hr  (3526) embryo 04-06hr  (2382) embryo 06-08hr  (2030) embryo 08-10hr  (1974) embryo 10-12hr    1372 embryo 12-14hr    1334 embryo 14-16hr  1413 embryo 16-18hr    1148 embryo 18-20hr    706 embryo 20-22hr  1495 embryo 22-24hr    1207 larva L1    438 larva L2    380 larva L3 12hr old    289 larva L3 puffstage 1-2    336 larva L3 puffstage 3-6    1143 larva L3 puffstage 7-9  (2383) white prepupae new  (2796) white prepupae 12hr  (1817) white prepupae 24hr    1209 pupae 2d postWPP    1155 pupae 3d postWPP    738 pupae 4d postWPP    839 adult male 01day    730 adult male 05day    578 adult male 30day    500 adult female 01day    1350 adult female 05day  (1938) adult female 30day  (1986) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    3549 embryo 02-04hr    3526 embryo 04-06hr    2382 embryo 06-08hr    2030 embryo 08-10hr    1974 embryo 10-12hr    1372 embryo 12-14hr    1334 embryo 14-16hr    1413 embryo 16-18hr    1148 embryo 18-20hr    706 embryo 20-22hr    1495 embryo 22-24hr    1207 larva L1    438 larva L2    380 larva L3 12hr old    289 larva L3 puffstage 1-2    336 larva L3 puffstage 3-6    1143 larva L3 puffstage 7-9    2383 white prepupae new    2796 white prepupae 12hr    1817 white prepupae 24hr    1209 pupae 2d postWPP    1155 pupae 3d postWPP    738 pupae 4d postWPP    839 adult male 01day    730 adult male 05day    578 adult male 30day    500 adult female 01day    1350 adult female 05day    1938 adult female 30day    1986 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    3549 embryo 02-04hr    3526 embryo 04-06hr    2382 embryo 06-08hr    2030 embryo 08-10hr    1974 embryo 10-12hr    1372 embryo 12-14hr    1334 embryo 14-16hr    1413 embryo 16-18hr    1148 embryo 18-20hr    706 embryo 20-22hr    1495 embryo 22-24hr    1207 larva L1    438 larva L2    380 larva L3 12hr old    289 larva L3 puffstage 1-2    336 larva L3 puffstage 3-6    1143 larva L3 puffstage 7-9    2383 white prepupae new    2796 white prepupae 12hr    1817 white prepupae 24hr    1209 pupae 2d postWPP    1155 pupae 3d postWPP    738 pupae 4d postWPP    839 adult male 01day    730 adult male 05day    578 adult male 30day    500 adult female 01day    1350 adult female 05day    1938 adult female 30day    1986 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0259168 expression level Developmental Stage   Expression Level embryo 00-02hr    3549 embryo 02-04hr    3526 embryo 04-06hr    2382 embryo 06-08hr    2030 embryo 08-10hr    1974 embryo 10-12hr    1372 embryo 12-14hr    1334 embryo 14-16hr    1413 embryo 16-18hr    1148 embryo 18-20hr    706 embryo 20-22hr    1495 embryo 22-24hr    1207 larva L1    438 larva L2    380 larva L3 12hr old    289 larva L3 puffstage 1-2    336 larva L3 puffstage 3-6    1143 larva L3 puffstage 7-9    2383 white prepupae new    2796 white prepupae 12hr    1817 white prepupae 24hr    1209 pupae 2d postWPP    1155 pupae 3d postWPP    738 pupae 4d postWPP    839 adult male 01day    730 adult male 05day    578 adult male 30day    500 adult female 01day    1350 adult female 05day    1938 adult female 30day    1986 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (3549) embryo 02-04hr  (3526) embryo 04-06hr  (2382) embryo 06-08hr  (2030) embryo 08-10hr  (1974) embryo 10-12hr  (1372) embryo 12-14hr  (1334) embryo 14-16hr  (1413) embryo 16-18hr  (1148) embryo 18-20hr  (706) embryo 20-22hr  (1495) embryo 22-24hr  (1207) larva L1  (438) larva L2  (380) larva L3 12hr old  (289) larva L3 puffstage 1-2  (336) larva L3 puffstage 3-6  (1143) larva L3 puffstage 7-9  (2383) white prepupae new  (2796) white prepupae 12hr  (1817) white prepupae 24hr  (1209) pupae 2d postWPP  (1155) pupae 3d postWPP  (738) pupae 4d postWPP  (839) adult male 01day  (730) adult male 05day  (578) adult male 30day  (500) adult female 01day  (1350) adult female 05day  (1938) adult female 30day  (1986) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (3549) embryo 02-04hr  (3526) embryo 04-06hr  2382 embryo 06-08hr  2030 embryo 08-10hr  1974 embryo 10-12hr    1372 embryo 12-14hr    1334 embryo 14-16hr  1413 embryo 16-18hr    1148 embryo 18-20hr    706 embryo 20-22hr  1495 embryo 22-24hr    1207 larva L1    438 larva L2    380 larva L3 12hr old    289 larva L3 puffstage 1-2    336 larva L3 puffstage 3-6    1143 larva L3 puffstage 7-9  2383 white prepupae new  (2796) white prepupae 12hr  1817 white prepupae 24hr    1209 pupae 2d postWPP    1155 pupae 3d postWPP    738 pupae 4d postWPP    839 adult male 01day    730 adult male 05day    578 adult male 30day    500 adult female 01day    1350 adult female 05day  1938 adult female 30day  1986 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    3549 embryo 02-04hr    3526 embryo 04-06hr    2382 embryo 06-08hr    2030 embryo 08-10hr    1974 embryo 10-12hr    1372 embryo 12-14hr    1334 embryo 14-16hr    1413 embryo 16-18hr    1148 embryo 18-20hr    706 embryo 20-22hr    1495 embryo 22-24hr    1207 larva L1    438 larva L2    380 larva L3 12hr old    289 larva L3 puffstage 1-2    336 larva L3 puffstage 3-6    1143 larva L3 puffstage 7-9    2383 white prepupae new    2796 white prepupae 12hr    1817 white prepupae 24hr    1209 pupae 2d postWPP    1155 pupae 3d postWPP    738 pupae 4d postWPP    839 adult male 01day    730 adult male 05day    578 adult male 30day    500 adult female 01day    1350 adult female 05day    1938 adult female 30day    1986 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    3549 embryo 02-04hr    3526 embryo 04-06hr    2382 embryo 06-08hr    2030 embryo 08-10hr    1974 embryo 10-12hr    1372 embryo 12-14hr    1334 embryo 14-16hr    1413 embryo 16-18hr    1148 embryo 18-20hr    706 embryo 20-22hr    1495 embryo 22-24hr    1207 larva L1    438 larva L2    380 larva L3 12hr old    289 larva L3 puffstage 1-2    336 larva L3 puffstage 3-6    1143 larva L3 puffstage 7-9    2383 white prepupae new    2796 white prepupae 12hr    1817 white prepupae 24hr    1209 pupae 2d postWPP    1155 pupae 3d postWPP    738 pupae 4d postWPP    839 adult male 01day    730 adult male 05day    578 adult male 30day    500 adult female 01day    1350 adult female 05day    1938 adult female 30day    1986 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0259168    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0259168 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Heatmap Tissue   Expression Level Larval Central Nervous System no informative data Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea no informative data Larval Carcass no informative data Adult Head no informative data Adult Eye no informative data Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary no informative data Adult Testis no informative data Adult Male Accessory Gland no informative data Adult Carcass no informative data modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_01
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0259168 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG7835-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | pupal stage, with Scer\GAL4pnr-MD237 mnbGD12298 viable mnbEY14320 viable, with Scer\GAL4pnr-MD237 mnbGD13197 Sterility Allele fertile mnbEY14320 Other Phenotypes Allele behavior defective mnb1 mnb2 mnb3 mnb4 visible, with Scer\GAL4pnr-MD237 mnbGD13197 Phenotype manifest in Allele embryonic/larval brain mnb1 mnb2 mnb3 mnb4 trichogen cell, with Scer\GAL4pnr-MD237 mnbGD13197
Classical Alleles ( 19 )
For All Classical Alleles Show
Allele of mnb Class Mutagen Stocks Known lesion mnbd10364 P-element activity 1 -- mnbd11431 P-element activity 1 -- mnbe02428 piggyBac transposase 1 -- mnbe03885 piggyBac transposase 1 -- mnbEY14320 P-element activity 1 -- mnbf01371 piggyBac transposase 1 -- mnbKG04573 1 -- mnbNP6003 1 -- mnbNP7209 1 -- mnbUM-8014-3 1 -- mnb1 ethyl methanesulfonate 0 Yes mnb2 PM hybrid dysgenesis 0 Yes mnb3 ethyl methanesulfonate 0 -- mnb4 X ray 0 Yes mnbf04483 piggyBac transposase 0 -- mnbGG01512 0 -- mnbGG01751 0 -- mnbGG01814 0 -- mnbUM-8181-3 0 --
Alleles Carried on Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Allele of mnb Class Mutagen Stocks Known lesion mnbGD12298 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes mnbGD13197 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes mnbGL00104 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes mnbHM05098 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes mnbJF01364 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes mnbKK102642 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes
Aneuploid Aberrations
Disrupted in	T(1;2)B27 (Baumann et al., 1987, Tejedor et al., 1995)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD13197} NA P{KK102642} NA P{TRiP.GL00104} NA P{TRiP.HM05098} NA P{TRiP.JF01364} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}mnbEY14320 NA P{Mae-UAS.6.11}mnbGG01512 NA P{Mae-UAS.6.11}mnbGG01751 NA P{Mae-UAS.6.11}mnbGG01814 NA P{XP}mnbd10364 NA P{XP}mnbd11431 NA miscellaneous insertions P{}mnb2 NA P{RS3}mnbUM-8014-3 NA P{RS3}mnbUM-8181-3 NA P{SUPor-P}mnbKG04573 NA PBac{RB}mnbe02428 NA PBac{RB}mnbe03885 NA PBac{WH}mnbf01371 NA PBac{WH}mnbf04483 NA insertion of enhancer trap binary system P{GawB}mnbNP6003 No P{GawB}mnbNP7209 No
Gene Ontology: Function, Process & Cellular Component ( 11 unique terms )
Terms Based on Experimental Evidence ( 5 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
brain development inferred from mutant phenotype (Heisenberg and Wolf, 1984, Fischbach and Heisenberg, 1984) circadian rhythm inferred from genetic interaction with so (Helfrich, 1986) nervous system development inferred from mutant phenotype (Tejedor et al., 1995) olfactory learning inferred from mutant phenotype (Heisenberg et al., 1985) visual behavior inferred from mutant phenotype (Tejedor et al., 1995)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 7 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290, InterPro:IPR017441 (FlyBase Curators et al., 2004-) protein kinase activity non-traceable author statement (Morrison et al., 2000) protein serine/threonine/tyrosine kinase activity inferred from sequence or structural similarity with EMBL:X79769 (FlyBase, 1992-) protein serine/threonine kinase activity inferred from electronic annotation with InterPro:IPR008271 (FlyBase Curators et al., 2004-) inferred from sequence or structural similarity (Tejedor et al., 1995) non-traceable author statement (Swiss-Prot Project Members, 1996.2.1)
Biological Process
CV term Evidence References
brain development non-traceable author statement (Swiss-Prot Project Members, 1996.2.1) learning or memory non-traceable author statement (Sokolowski, 2001) protein phosphorylation inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290, InterPro:IPR008271 (FlyBase Curators et al., 2004-) non-traceable author statement (Morrison et al., 2000)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions mnb allele Gene References mnb1 rol (Coombe, 1986)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 59093 DroID - A comprehensive database of gene and protein interactions. • FBgn0259168 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 32771
Orthologs
Genome-wide drosophilid orthologs	Dana\GF20860 Dere\GG18154 Dgri\GH12565 Dmoj\GI16344 Dper\GL14559 Dpse\GA20611 Dsec\GM22846 Dsim\GD15658 Dvir\GJ15681 Dwil\GK10086 Dyak\GE15563
Curated drosophilid orthologs	Dpse\GA20611   Dpse\GA20619
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AGAP002119-PA Anopheles gambiae (mosquito) At5g35980.1 Arabidopsis thaliana (wall cress) CAL0006406 Candida albicans (a form of yeast) ENSCAFP00000014403 Canis familiaris (dog) 153454 Chlamidomonas reinhardtii (green alga) 82340 Capitella spl (polychaete worm) DDB0229429 Dictyostelium discoideum (amoeba) DEHA2F08096g Debaryomyces Hansenii (yeast) ENSDARP00000007662 Danio rerio (zebra fish) ENSGACP00000013799 Gymnopilus aculeatus (mushroom) 188308 Hypsipyla robusta (cedar shoot borer) 7973 Monocosta brevicollis (choanoflagellate) ENSMODP00000026246 Monodelphis domestica (gray opossum) XP_001606660.1 Nasonia vitripennis (wasp) ENSOANP00000006579 Ornithorynchus anatinus (platypus) ENSORLP00000006824 Oryzias latipes (japanese killifish) PHUM002445-PA Pediculus humanus (human body louse) PP25390 Pristionchus pacificus (nematode) 5004651 Sorghum bicolor (sorghum) XP_786330.2 Strongylocentrotus purpuratus (purple sea urchin) ENSTRUP00000009230 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000028566 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB15413 NV11530 LH13242 ISCW022190 FBgn0233135 FBgn0212101 FBgn0202869 FBgn0187320 FBgn0177716 JGI_V11_915 FBgn0080605 FBgn0152164 FBgn0139092 FBgn0120044 FBgn0110370 FBgn0097866 CPIJ010268 CPIJ019411 ACYPI002557 GB20129 AGAP002119 AAEL003400 AAEL003370 PHUM284180 GLEAN_07717
Stocks & Reagents
Stocks Listed in FlyBase ( 17 )
Bloomington	13526 y1 P{SUPor-P}mnbKG04573 28888 y1 v1; P{TRiP.HM05098}attP2
Harvard	- P{XP}mnbd10364 - P{XP}mnbd11431 - PBac{RB}mnbe02428 - PBac{RB}mnbe03885 - PBac{WH}mnbf01371
Kyoto	124281 w1118 P{RS3}mnbUM-8014-3
VDRC	v107066 P{KK102642}VIE-260B v35283 w1118; P{GD12298}v35283 v28628 w1118; P{GD13197}v28628/TM3 v35282 w1118; P{GD12298}v35282/TM3
	More stocks available...
Genomic Clones ( 2 )
	BACR01N10 BACR29H04 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 74 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD34846 LP07621
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GM03850 GM28780 LD09329 LD15111 LD17936 LP04638 LP13983 RE39566 RH44787 RH55865 SD13331
Other clones	8328 13043 20072 34813 37941 44421 54033 60477 66291 70383 72748 79214 123230 174232 191345 214225.49 214345.14 274182 283589 CK01.3C.F9 CK01.4A.D2 CK01.74B.E12 CK01.81B.E11 CK01.87B.F8 CK01.88B.H1 CK01.102C.F7 CK01.112D.G6 CK01.117A.G11 CK01.144B.D1 EC052179 EC052689 EC053180 EC054185 EK009743 EK058352 EK062720 EK069056 EK086324 EK100202 EK114510 EK117120 EK118813 EK134035 EK142704 EK144910 EK159628 EK178248 EK185240 EK194063 EK198058 EK201322 EK208744 EK246341 EK294058 EK301721 EP02411 EP11535 EP16746 EP22255 FGC012C03 FGH129F06
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0259168 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32771 32772
Antibody Information
	polyclonal (Tejedor et al., 1995, Tejedor et al., 1995)
Other Information
Discoverer
	M. Heisenberg.
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: mnb CG7835 (FlyBase Genome Annotators, 2008)
Additional comments	Annotations CG7826 and CG7835 merged as CG42273 in release 5.8 of the genome annotation. (FlyBase Genome Annotators, 2008) Apparently part of 'Sh complex'. Seems to be allelic to Sh, given map position and mnb-associated leg shaking (A. Ferrus and M. Heisenberg, unpublished); yet, Sh mutations do not lead to any grossly aberrant brain morphology and Sh5 plus Sh14 complement mnb1 with regard to anatomical defects caused by the latter (M. Heisenberg, unpublished); mnb1 also complements lethal alleles of 3 genes mapping just distally to Sh alleles (see Tanouye, Lam and Iverson, 1986), i.e., l(1)16Fa, l(1)16Fb and l(1)16Fc; an X-linked enhancer of mnb has been identified, which causes lethality when expressed along with the latter (M. Heisenberg, unpublished); the former maps near cv and (when in combination with mnb1) leads to death late in pupation (mutants can be 'rescued' by opening the pupal case).
Other Comments
	When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an increase in the proportion of S phase cells and/or aneuploidy, an increase in the proportion of G2/M phase cells, an increase in mitotic index and abnormal spindles are seen. (Bettencourt-Dias et al., 2004) Mutations in mnb cause an abnormal spacing of neuroblasts in the outer proliferation centre of the larval brain. As a consequence the larval brain exhibits a specific and marked reduction of the optic lobes and central brain hemispheres. The insufficient number of distinct neurons in the brain is correlated with specific abnormalities in visual and olfactory behaviour. (Tejedor et al., 1995) mnb is important for the viability of neurons in the brain. (Zhu et al., 1991) Defined during a molecular analysis of Sh. (Baumann et al., 1987) mnb, when in combination with rol and sol, shows diminished amplitudes of light-on and light-off transient spikes. (Coombe, 1986) In tests of locomotor activity rhythms, singly mutant mnb adults are basically normal, but a high proportion of mnb so double mutants show complex rhythmicities (dual circadian periodicities) with about 20% being arrhythmic (3 times greater than wild type). (Helfrich, 1986) Behavioural and anatomical studies demonstrate that central brain lesions can be interpreted behaviourally. Mushroom bodies have normal size and shape. Whole brain is reduced in volume, flies are incapable of olfactory learning. (Heisenberg et al., 1985) Brain mass approximately half normal, including smaller than normal optic lobes and reduction in cell number, with the exceptions of the neuropil of the lamina optic ganglion which appears normal in size and the general morphology and peduncle of mushroom body which has apparently normal number of fibers. (Heisenberg and Wolf, 1984, Fischbach and Heisenberg, 1984) Isolation: A mutagenized X provided by J. Merriam.
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_167581 NM_170658 NM_170659 NM_001014750 RefSeq (Proteins) • NP_728104 NP_728106 NP_728107 NP_001014750 DDBJ / EMBL / GenBank • AAF48777 AAF48777.3 AAF48778 AAL28966 AAN09442 AAN09442.1 AAT94484 AAX52504 AI061748 AY061418 BT015255 CAA50065 CAA50068 CAA50069 CW848256 CZ477233 CZ477234 CZ477718 CZ477719 CZ480244 CZ481640 CZ481641 CZ483846 CZ487019 CZ487020 X70794 X70798 X70799 Entrez Gene - A searchable database of RefSeq genes. • 32771 UniProtKB/Swiss-Prot • P49657 UniProtKB/TrEMBL • Q9VX06
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, catalytic domain (IPR000719) Serine/threonine-protein kinase, catalytic domain (IPR002290) Serine/threonine-protein kinase, active site (IPR008271) Protein kinase-like domain (IPR011009) Protein kinase, ATP binding site (IPR017441) Serine/threonine-protein kinase-like domain (IPR017442)
Linkouts
	BioGRID - A database of protein and genetic interactions • 59093 DroID - A comprehensive database of gene and protein interactions. • FBgn0259168 DRSC - Results from RNAi screens. • FBgn0259168 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0259168 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG7835-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0259168 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32771 32772 InParanoid A subset of ortholog calls from InParanoid. • AGAP002119-PA Anopheles gambiae (mosquito) At5g35980.1 Arabidopsis thaliana (wall cress) CAL0006406 Candida albicans (a form of yeast) ENSCAFP00000014403 Canis familiaris (dog) 153454 Chlamidomonas reinhardtii (green alga) 82340 Capitella spl (polychaete worm) DDB0229429 Dictyostelium discoideum (amoeba) DEHA2F08096g Debaryomyces Hansenii (yeast) ENSDARP00000007662 Danio rerio (zebra fish) ENSGACP00000013799 Gymnopilus aculeatus (mushroom) 188308 Hypsipyla robusta (cedar shoot borer) 7973 Monocosta brevicollis (choanoflagellate) ENSMODP00000026246 Monodelphis domestica (gray opossum) XP_001606660.1 Nasonia vitripennis (wasp) ENSOANP00000006579 Ornithorynchus anatinus (platypus) ENSORLP00000006824 Oryzias latipes (japanese killifish) PHUM002445-PA Pediculus humanus (human body louse) PP25390 Pristionchus pacificus (nematode) 5004651 Sorghum bicolor (sorghum) XP_786330.2 Strongylocentrotus purpuratus (purple sea urchin) ENSTRUP00000009230 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000028566 Xenopus tropicalis (frog) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /neural/minibrn1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 32771 modMine - Data generated by the modENCODE project. • FBgn0259168 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB15413 NV11530 LH13242 ISCW022190 FBgn0233135 FBgn0212101 FBgn0202869 FBgn0187320 FBgn0177716 JGI_V11_915 FBgn0080605 FBgn0152164 FBgn0139092 FBgn0120044 FBgn0110370 FBgn0097866 CPIJ010268 CPIJ019411 ACYPI002557 GB20129 AGAP002119 AAEL003400 AAEL003370 PHUM284180 GLEAN_07717
Synonyms & Secondary IDs ( 13 )
Reported As
Symbol Synonym	CG7826 (Morrison et al., 2000, Keleman et al., 2009.8.5) CG7835 (Barmina et al., 2005) CG42273 (FlyBase Genome Annotators, 2008, Perkins et al., 2009, Ni et al., 2008, Ni et al., 2011.7.19) Dyrk1 (Manning, 2001.10.15) DYRK1 (Mattila et al., 2008) ME-IV (Ferrus, 1999.3.4, Prado et al., 1999) min   minibrain (Edwards et al., 2006) Mnb (Colonques et al., 2001, Ceron et al., 1997) mnb (Slade et al., 2007, Legube et al., 2006, Gabut et al., 2007, Baron et al., 2009, Seong et al., 2011) MNB (Kinstrie et al., 2006, Mattila et al., 2008) unnamed (Baumann et al., 1987)
Name Synonym	CG7835   CG42273 (FlyBase Genome Annotators, 2008) minibrain (Edwards et al., 2006, Gabut et al., 2007, Baron et al., 2009) Minibrain (Colonques et al., 2001, Kinstrie et al., 2006)
Secondary FlyBase IDs
	FBgn0002777 FBgn0030879
References ( 66 )
Generate a list of	All references relating to this gene ( 66 )
List References by type	Research paper  ( 27 ) Supplementary material  ( 4 ) Review  ( 11 ) Personal communication to FlyBase  ( 7 ) Abstract  ( 5 ) FlyBase analysis  ( 3 ) Book  ( 1 ) DNA/RNA sequence record  ( 1 ) Protein sequence record  ( 1 ) Computer file  ( 3 ) Automatic genome annotation  ( 1 ) Curated genome annotation  ( 1 ) Letter  ( 1 )
Recent research papers ( 1 )
	Seong et al., 2011, Biogerontology 12(2): 93--107 Genome-wide analysis of low-dose irradiated male Drosophila melanogaster with extended longevity. [FBrf0213307] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...