Gene Dmel\mnb

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

mnb-PE

FBpp0289735

95.9

908

9.11

NP_728104

AAF48777

mnb-PF

FBpp0288894

66.3

607

9.49

NP_728106

AAN09442

mnb-PG

FBpp0289736

65.9

604

9.28

NP_001014750

AAX52504

mnb-PH

FBpp0289737

111.1

1047

8.45

NP_728107

AAF48778

mnb-PI

FBpp0309627

81.1

743

8.49

Additional Polypeptide Data & Comments

Reported size (kDa)

843, 542, 539 (aa); 90, 60, 58 (kD observed); 89, 59.4, 59.1 (kD predicted)

Comments

External Data

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Protein kinase, catalytic domain (IPR000719)

Serine/threonine- / dual specificity protein kinase, catalytic domain (IPR002290)

Serine/threonine-protein kinase, active site (IPR008271)

Protein kinase-like domain (IPR011009)

Protein kinase, ATP binding site (IPR017441)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P49657

UniProtKB/TrEMBL

Q9VX06

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Crossreferences

Expression Data

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

ventral nerve cord

supraesophageal ganglion

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

embryonic/larval optic lobe

mushroom body

larval outer optic anlage

adult stage

pigment cell

retina

mushroom body beta-lobe

adult mushroom body

adult mushroom body gamma-lobe

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

larval stage & pupal stage

Additional Descriptive Data

Higher levels of the 843aa form of mnb protein are observed in embryos and pupae than larvae.

Higher levels of the 542aa form of mnb protein are observed in embryos and pupae than larvae.

The 539aa form of mnb is expressed most strongly in larvae and pupae and is the predominant form in third instar larvae.

mnb protein is most prominent in the mushroom body neuropil and the outer proliferation centers of the optic lobes in the larval brain. In adult brains, the level of mnb protein is low in the optic lobes and central brain hemispheres but is high in retinal pigment cells as well as in the α, β, and γ cells and the peduncle of the mushroom bodies.

Marker for

Subcellular Localization (GO Cellular Component)

CV term

(The modENCODE Consortium, 2010, Graveley et al., 2011)

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		20
central nervous system, larvae L3		26
central nervous system, pupae P8		18
head, virgin 1-day female		6
head, virgin 4-day female		6
head, virgin 20-day female		5
head, mated 1-day female		6
head, mated 4-day female		6
head, mated 20-day female		5
head, mated 1-day male		8
head, mated 4-day male		8
head, mated 20-day male		8
salivary gland, larvae L3 wandering		10
salivary gland, white prepupae		122
digestive system, larvae L3 wandering		16
digestive system, 1-day adult		3
digestive system, 4-day adult		6
digestive system, 20-day adult		5
fat body, larvae L3 wandering		3
fat body, white prepupae		15
fat body, pupae P8		7
carcass, larvae L3 wandering		28
carcass, 1-day adult		9
carcass, 4-day adult		8
carcass, 20-day adult		6
ovary, virgin 4-day female		42
ovary, mated 4-day female		36
testis, mated 4-day male		4
accessory gland, mated 4-day male		3
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		20
central nervous system, larvae L3		26
central nervous system, pupae P8		18
head, virgin 1-day female		6
head, virgin 4-day female		6
head, virgin 20-day female		5
head, mated 1-day female		6
head, mated 4-day female		6
head, mated 20-day female		5
head, mated 1-day male		8
head, mated 4-day male		8
head, mated 20-day male		8
salivary gland, larvae L3 wandering		10
salivary gland, white prepupae		(122)
digestive system, larvae L3 wandering		16
digestive system, 1-day adult		3
digestive system, 4-day adult		6
digestive system, 20-day adult		5
fat body, larvae L3 wandering		3
fat body, white prepupae		15
fat body, pupae P8		7
carcass, larvae L3 wandering		(28)
carcass, 1-day adult		9
carcass, 4-day adult		8
carcass, 20-day adult		6
ovary, virgin 4-day female		(42)
ovary, mated 4-day female		(36)
testis, mated 4-day male		4
accessory gland, mated 4-day male		3
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		20
central nervous system, larvae L3		26
central nervous system, pupae P8		18
head, virgin 1-day female		6
head, virgin 4-day female		6
head, virgin 20-day female		5
head, mated 1-day female		6
head, mated 4-day female		6
head, mated 20-day female		5
head, mated 1-day male		8
head, mated 4-day male		8
head, mated 20-day male		8
salivary gland, larvae L3 wandering		10
salivary gland, white prepupae		(122)
digestive system, larvae L3 wandering		16
digestive system, 1-day adult		3
digestive system, 4-day adult		6
digestive system, 20-day adult		5
fat body, larvae L3 wandering		3
fat body, white prepupae		15
fat body, pupae P8		7
carcass, larvae L3 wandering		28
carcass, 1-day adult		9
carcass, 4-day adult		8
carcass, 20-day adult		6
ovary, virgin 4-day female		42
ovary, mated 4-day female		36
testis, mated 4-day male		4
accessory gland, mated 4-day male		3
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		20
central nervous system, larvae L3		26
central nervous system, pupae P8		18
head, virgin 1-day female		6
head, virgin 4-day female		6
head, virgin 20-day female		5
head, mated 1-day female		6
head, mated 4-day female		6
head, mated 20-day female		5
head, mated 1-day male		8
head, mated 4-day male		8
head, mated 20-day male		8
salivary gland, larvae L3 wandering		10
salivary gland, white prepupae		122
digestive system, larvae L3 wandering		16
digestive system, 1-day adult		3
digestive system, 4-day adult		6
digestive system, 20-day adult		5
fat body, larvae L3 wandering		3
fat body, white prepupae		15
fat body, pupae P8		7
carcass, larvae L3 wandering		28
carcass, 1-day adult		9
carcass, 4-day adult		8
carcass, 20-day adult		6
ovary, virgin 4-day female		42
ovary, mated 4-day female		36
testis, mated 4-day male		4
accessory gland, mated 4-day male		3
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		20
central nervous system, larvae L3		26
central nervous system, pupae P8		18
head, virgin 1-day female		6
head, virgin 4-day female		6
head, virgin 20-day female		5
head, mated 1-day female		6
head, mated 4-day female		6
head, mated 20-day female		5
head, mated 1-day male		8
head, mated 4-day male		8
head, mated 20-day male		8
salivary gland, larvae L3 wandering		10
salivary gland, white prepupae		122
digestive system, larvae L3 wandering		16
digestive system, 1-day adult		3
digestive system, 4-day adult		6
digestive system, 20-day adult		5
fat body, larvae L3 wandering		3
fat body, white prepupae		15
fat body, pupae P8		7
carcass, larvae L3 wandering		28
carcass, 1-day adult		9
carcass, 4-day adult		8
carcass, 20-day adult		6
ovary, virgin 4-day female		42
ovary, mated 4-day female		36
testis, mated 4-day male		4
accessory gland, mated 4-day male		3
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		20
central nervous system, larvae L3		26
central nervous system, pupae P8		18
head, virgin 1-day female		6
head, virgin 4-day female		6
head, virgin 20-day female		5
head, mated 1-day female		6
head, mated 4-day female		6
head, mated 20-day female		5
head, mated 1-day male		8
head, mated 4-day male		8
head, mated 20-day male		8
salivary gland, larvae L3 wandering		10
salivary gland, white prepupae		(122)
digestive system, larvae L3 wandering		16
digestive system, 1-day adult		3
digestive system, 4-day adult		6
digestive system, 20-day adult		5
fat body, larvae L3 wandering		3
fat body, white prepupae		15
fat body, pupae P8		7
carcass, larvae L3 wandering		28
carcass, 1-day adult		9
carcass, 4-day adult		8
carcass, 20-day adult		6
ovary, virgin 4-day female		(42)
ovary, mated 4-day female		(36)
testis, mated 4-day male		4
accessory gland, mated 4-day male		3
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		20
central nervous system, larvae L3		26
central nervous system, pupae P8		18
head, virgin 1-day female		6
head, virgin 4-day female		6
head, virgin 20-day female		5
head, mated 1-day female		6
head, mated 4-day female		6
head, mated 20-day female		5
head, mated 1-day male		8
head, mated 4-day male		8
head, mated 20-day male		8
salivary gland, larvae L3 wandering		10
salivary gland, white prepupae		122
digestive system, larvae L3 wandering		16
digestive system, 1-day adult		3
digestive system, 4-day adult		6
digestive system, 20-day adult		5
fat body, larvae L3 wandering		3
fat body, white prepupae		15
fat body, pupae P8		7
carcass, larvae L3 wandering		28
carcass, 1-day adult		9
carcass, 4-day adult		8
carcass, 20-day adult		6
ovary, virgin 4-day female		42
ovary, mated 4-day female		36
testis, mated 4-day male		4
accessory gland, mated 4-day male		3
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		20
central nervous system, larvae L3		26
central nervous system, pupae P8		18
head, virgin 1-day female		6
head, virgin 4-day female		6
head, virgin 20-day female		5
head, mated 1-day female		6
head, mated 4-day female		6
head, mated 20-day female		5
head, mated 1-day male		8
head, mated 4-day male		8
head, mated 20-day male		8
salivary gland, larvae L3 wandering		10
salivary gland, white prepupae		122
digestive system, larvae L3 wandering		16
digestive system, 1-day adult		3
digestive system, 4-day adult		6
digestive system, 20-day adult		5
fat body, larvae L3 wandering		3
fat body, white prepupae		15
fat body, pupae P8		7
carcass, larvae L3 wandering		28
carcass, 1-day adult		9
carcass, 4-day adult		8
carcass, 20-day adult		6
ovary, virgin 4-day female		42
ovary, mated 4-day female		36
testis, mated 4-day male		4
accessory gland, mated 4-day male		3
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed at stages throughout embryogenesis, during late larval stages, during early pupal stages, in adult female stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		47
embryo 02-04hr		47
embryo 04-06hr		32
embryo 06-08hr		27
embryo 08-10hr		26
embryo 10-12hr		18
embryo 12-14hr		18
embryo 14-16hr		19
embryo 16-18hr		15
embryo 18-20hr		9
embryo 20-22hr		20
embryo 22-24hr		16
larva L1		6
larva L2		5
larva L3 12hr old		4
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		15
larva L3 puffstage 7-9		32
white prepupae new		37
white prepupae 12hr		24
white prepupae 24hr		16
pupae 2d postWPP		15
pupae 3d postWPP		10
pupae 4d postWPP		11
adult male 01day		10
adult male 05day		8
adult male 30day		7
adult female 01day		18
adult female 05day		26
adult female 30day		26
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(47)
embryo 02-04hr		(47)
embryo 04-06hr		(32)
embryo 06-08hr		(27)
embryo 08-10hr		26
embryo 10-12hr		18
embryo 12-14hr		18
embryo 14-16hr		19
embryo 16-18hr		15
embryo 18-20hr		9
embryo 20-22hr		20
embryo 22-24hr		16
larva L1		6
larva L2		5
larva L3 12hr old		4
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		15
larva L3 puffstage 7-9		(32)
white prepupae new		(37)
white prepupae 12hr		24
white prepupae 24hr		16
pupae 2d postWPP		15
pupae 3d postWPP		10
pupae 4d postWPP		11
adult male 01day		10
adult male 05day		8
adult male 30day		7
adult female 01day		18
adult female 05day		26
adult female 30day		26
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		47
embryo 02-04hr		47
embryo 04-06hr		32
embryo 06-08hr		27
embryo 08-10hr		26
embryo 10-12hr		18
embryo 12-14hr		18
embryo 14-16hr		19
embryo 16-18hr		15
embryo 18-20hr		9
embryo 20-22hr		20
embryo 22-24hr		16
larva L1		6
larva L2		5
larva L3 12hr old		4
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		15
larva L3 puffstage 7-9		32
white prepupae new		37
white prepupae 12hr		24
white prepupae 24hr		16
pupae 2d postWPP		15
pupae 3d postWPP		10
pupae 4d postWPP		11
adult male 01day		10
adult male 05day		8
adult male 30day		7
adult female 01day		18
adult female 05day		26
adult female 30day		26
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		47
embryo 02-04hr		47
embryo 04-06hr		32
embryo 06-08hr		27
embryo 08-10hr		26
embryo 10-12hr		18
embryo 12-14hr		18
embryo 14-16hr		19
embryo 16-18hr		15
embryo 18-20hr		9
embryo 20-22hr		20
embryo 22-24hr		16
larva L1		6
larva L2		5
larva L3 12hr old		4
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		15
larva L3 puffstage 7-9		32
white prepupae new		37
white prepupae 12hr		24
white prepupae 24hr		16
pupae 2d postWPP		15
pupae 3d postWPP		10
pupae 4d postWPP		11
adult male 01day		10
adult male 05day		8
adult male 30day		7
adult female 01day		18
adult female 05day		26
adult female 30day		26
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		47
embryo 02-04hr		47
embryo 04-06hr		32
embryo 06-08hr		27
embryo 08-10hr		26
embryo 10-12hr		18
embryo 12-14hr		18
embryo 14-16hr		19
embryo 16-18hr		15
embryo 18-20hr		9
embryo 20-22hr		20
embryo 22-24hr		16
larva L1		6
larva L2		5
larva L3 12hr old		4
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		15
larva L3 puffstage 7-9		32
white prepupae new		37
white prepupae 12hr		24
white prepupae 24hr		16
pupae 2d postWPP		15
pupae 3d postWPP		10
pupae 4d postWPP		11
adult male 01day		10
adult male 05day		8
adult male 30day		7
adult female 01day		18
adult female 05day		26
adult female 30day		26
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(47)
embryo 02-04hr		(47)
embryo 04-06hr		32
embryo 06-08hr		27
embryo 08-10hr		26
embryo 10-12hr		18
embryo 12-14hr		18
embryo 14-16hr		19
embryo 16-18hr		15
embryo 18-20hr		9
embryo 20-22hr		20
embryo 22-24hr		16
larva L1		6
larva L2		5
larva L3 12hr old		4
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		15
larva L3 puffstage 7-9		32
white prepupae new		(37)
white prepupae 12hr		24
white prepupae 24hr		16
pupae 2d postWPP		15
pupae 3d postWPP		10
pupae 4d postWPP		11
adult male 01day		10
adult male 05day		8
adult male 30day		7
adult female 01day		18
adult female 05day		26
adult female 30day		26
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		47
embryo 02-04hr		47
embryo 04-06hr		32
embryo 06-08hr		27
embryo 08-10hr		26
embryo 10-12hr		18
embryo 12-14hr		18
embryo 14-16hr		19
embryo 16-18hr		15
embryo 18-20hr		9
embryo 20-22hr		20
embryo 22-24hr		16
larva L1		6
larva L2		5
larva L3 12hr old		4
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		15
larva L3 puffstage 7-9		32
white prepupae new		37
white prepupae 12hr		24
white prepupae 24hr		16
pupae 2d postWPP		15
pupae 3d postWPP		10
pupae 4d postWPP		11
adult male 01day		10
adult male 05day		8
adult male 30day		7
adult female 01day		18
adult female 05day		26
adult female 30day		26
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		47
embryo 02-04hr		47
embryo 04-06hr		32
embryo 06-08hr		27
embryo 08-10hr		26
embryo 10-12hr		18
embryo 12-14hr		18
embryo 14-16hr		19
embryo 16-18hr		15
embryo 18-20hr		9
embryo 20-22hr		20
embryo 22-24hr		16
larva L1		6
larva L2		5
larva L3 12hr old		4
larva L3 puffstage 1-2		4
larva L3 puffstage 3-6		15
larva L3 puffstage 7-9		32
white prepupae new		37
white prepupae 12hr		24
white prepupae 24hr		16
pupae 2d postWPP		15
pupae 3d postWPP		10
pupae 4d postWPP		11
adult male 01day		10
adult male 05day		8
adult male 30day		7
adult female 01day		18
adult female 05day		26
adult female 30day		26
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		14
Schneider line 2 Sg4		8
embryonic 1182-4H		15
embryonic GM2		11
embryonic Kc167		20
embryonic S1		14
embryonic S3		7
leg disc CME L1		8
wing disc CME-W2		6
wing disc ML-DmD8		13
wing disc ML-DmD9		9
wing disc ML-DmD16-c3		13
wing disc ML-DmD21		10
wing disc ML-DmD32		12
haltere disc ML-DmD17-c3		12
eye-antennal disc ML-DmD11		8
antennal disc ML-DmD20-c5		5
mixed discs ML-DmD4-c1		9
CNS ML-DmBG1-c1		12
CNS ML-DmBG2-c2		14
tumorous blood cells mbn2		10
ovary fGS/OSS		12
ovary OSC		21
ovary OSS		4
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		14
Schneider line 2 Sg4		8
embryonic 1182-4H		15
embryonic GM2		11
embryonic Kc167		20
embryonic S1		14
embryonic S3		7
leg disc CME L1		8
wing disc CME-W2		6
wing disc ML-DmD8		13
wing disc ML-DmD9		9
wing disc ML-DmD16-c3		13
wing disc ML-DmD21		10
wing disc ML-DmD32		12
haltere disc ML-DmD17-c3		12
eye-antennal disc ML-DmD11		8
antennal disc ML-DmD20-c5		5
mixed discs ML-DmD4-c1		9
CNS ML-DmBG1-c1		12
CNS ML-DmBG2-c2		14
tumorous blood cells mbn2		10
ovary fGS/OSS		12
ovary OSC		21
ovary OSS		4
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		14
Schneider line 2 Sg4		8
embryonic 1182-4H		15
embryonic GM2		11
embryonic Kc167		20
embryonic S1		14
embryonic S3		7
leg disc CME L1		8
wing disc CME-W2		6
wing disc ML-DmD8		13
wing disc ML-DmD9		9
wing disc ML-DmD16-c3		13
wing disc ML-DmD21		10
wing disc ML-DmD32		12
haltere disc ML-DmD17-c3		12
eye-antennal disc ML-DmD11		8
antennal disc ML-DmD20-c5		5
mixed discs ML-DmD4-c1		9
CNS ML-DmBG1-c1		12
CNS ML-DmBG2-c2		14
tumorous blood cells mbn2		10
ovary fGS/OSS		12
ovary OSC		21
ovary OSS		4
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		14
Schneider line 2 Sg4		8
embryonic 1182-4H		15
embryonic GM2		11
embryonic Kc167		20
embryonic S1		14
embryonic S3		7
leg disc CME L1		8
wing disc CME-W2		6
wing disc ML-DmD8		13
wing disc ML-DmD9		9
wing disc ML-DmD16-c3		13
wing disc ML-DmD21		10
wing disc ML-DmD32		12
haltere disc ML-DmD17-c3		12
eye-antennal disc ML-DmD11		8
antennal disc ML-DmD20-c5		5
mixed discs ML-DmD4-c1		9
CNS ML-DmBG1-c1		12
CNS ML-DmBG2-c2		14
tumorous blood cells mbn2		10
ovary fGS/OSS		12
ovary OSC		21
ovary OSS		4
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		14
Schneider line 2 Sg4		8
embryonic 1182-4H		15
embryonic GM2		11
embryonic Kc167		20
embryonic S1		14
embryonic S3		7
leg disc CME L1		8
wing disc CME-W2		6
wing disc ML-DmD8		13
wing disc ML-DmD9		9
wing disc ML-DmD16-c3		13
wing disc ML-DmD21		10
wing disc ML-DmD32		12
haltere disc ML-DmD17-c3		12
eye-antennal disc ML-DmD11		8
antennal disc ML-DmD20-c5		5
mixed discs ML-DmD4-c1		9
CNS ML-DmBG1-c1		12
CNS ML-DmBG2-c2		14
tumorous blood cells mbn2		10
ovary fGS/OSS		12
ovary OSC		21
ovary OSS		4
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		14
Schneider line 2 Sg4		8
embryonic 1182-4H		15
embryonic GM2		11
embryonic Kc167		20
embryonic S1		14
embryonic S3		7
leg disc CME L1		8
wing disc CME-W2		6
wing disc ML-DmD8		13
wing disc ML-DmD9		9
wing disc ML-DmD16-c3		13
wing disc ML-DmD21		10
wing disc ML-DmD32		12
haltere disc ML-DmD17-c3		12
eye-antennal disc ML-DmD11		8
antennal disc ML-DmD20-c5		5
mixed discs ML-DmD4-c1		9
CNS ML-DmBG1-c1		12
CNS ML-DmBG2-c2		14
tumorous blood cells mbn2		10
ovary fGS/OSS		12
ovary OSC		21
ovary OSS		4
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		14
Schneider line 2 Sg4		8
embryonic 1182-4H		15
embryonic GM2		11
embryonic Kc167		20
embryonic S1		14
embryonic S3		7
leg disc CME L1		8
wing disc CME-W2		6
wing disc ML-DmD8		13
wing disc ML-DmD9		9
wing disc ML-DmD16-c3		13
wing disc ML-DmD21		10
wing disc ML-DmD32		12
haltere disc ML-DmD17-c3		12
eye-antennal disc ML-DmD11		8
antennal disc ML-DmD20-c5		5
mixed discs ML-DmD4-c1		9
CNS ML-DmBG1-c1		12
CNS ML-DmBG2-c2		14
tumorous blood cells mbn2		10
ovary fGS/OSS		12
ovary OSC		21
ovary OSS		4
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		14
Schneider line 2 Sg4		8
embryonic 1182-4H		15
embryonic GM2		11
embryonic Kc167		20
embryonic S1		14
embryonic S3		7
leg disc CME L1		8
wing disc CME-W2		6
wing disc ML-DmD8		13
wing disc ML-DmD9		9
wing disc ML-DmD16-c3		13
wing disc ML-DmD21		10
wing disc ML-DmD32		12
haltere disc ML-DmD17-c3		12
eye-antennal disc ML-DmD11		8
antennal disc ML-DmD20-c5		5
mixed discs ML-DmD4-c1		9
CNS ML-DmBG1-c1		12
CNS ML-DmBG2-c2		14
tumorous blood cells mbn2		10
ovary fGS/OSS		12
ovary OSC		21
ovary OSS		4
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		15
cold shock, 4-day adult		12
heat shock, 4-day adult		12
Cadmium 50 mM 6 hrs, larvae L3		3
Cadmium 50 mM 12 hrs, larvae L3		6
Cadmium 50 mM 48 hrs, 4-day adult		11
Cadmium 100 mM 48 hrs, 4-day adult		31
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		49
Zinc 5 mM 12 hrs, larvae L3		6
Zinc 4.5 mM 48 hrs, 4-day adult		13
Ethanol 2.5% 3 hrs, larvae L3		24
Ethanol 5% 3 hrs, larvae L3		3
Ethanol 10% 3 hrs, larvae L3		4
Caffeine 1.5 mg/ml 4 hrs, larvae L3		4
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		29
Caffeine 25 mg/ml 48 hrs, 4-day adult		16
Paraquat 5 mM 48 hrs, 4-day adult		20
Paraquat 10 mM 48 hrs, 4-day adult		18
Rotenone 2 μg 12 hrs, larvae L3		3
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		15
cold shock, 4-day adult		12
heat shock, 4-day adult		12
Cadmium 50 mM 6 hrs, larvae L3		3
Cadmium 50 mM 12 hrs, larvae L3		6
Cadmium 50 mM 48 hrs, 4-day adult		11
Cadmium 100 mM 48 hrs, 4-day adult		(31)
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		(49)
Zinc 5 mM 12 hrs, larvae L3		6
Zinc 4.5 mM 48 hrs, 4-day adult		13
Ethanol 2.5% 3 hrs, larvae L3		24
Ethanol 5% 3 hrs, larvae L3		3
Ethanol 10% 3 hrs, larvae L3		4
Caffeine 1.5 mg/ml 4 hrs, larvae L3		4
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(29)
Caffeine 25 mg/ml 48 hrs, 4-day adult		16
Paraquat 5 mM 48 hrs, 4-day adult		20
Paraquat 10 mM 48 hrs, 4-day adult		18
Rotenone 2 μg 12 hrs, larvae L3		3
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		15
cold shock, 4-day adult		12
heat shock, 4-day adult		12
Cadmium 50 mM 6 hrs, larvae L3		3
Cadmium 50 mM 12 hrs, larvae L3		6
Cadmium 50 mM 48 hrs, 4-day adult		11
Cadmium 100 mM 48 hrs, 4-day adult		31
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		49
Zinc 5 mM 12 hrs, larvae L3		6
Zinc 4.5 mM 48 hrs, 4-day adult		13
Ethanol 2.5% 3 hrs, larvae L3		24
Ethanol 5% 3 hrs, larvae L3		3
Ethanol 10% 3 hrs, larvae L3		4
Caffeine 1.5 mg/ml 4 hrs, larvae L3		4
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		29
Caffeine 25 mg/ml 48 hrs, 4-day adult		16
Paraquat 5 mM 48 hrs, 4-day adult		20
Paraquat 10 mM 48 hrs, 4-day adult		18
Rotenone 2 μg 12 hrs, larvae L3		3
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		15
cold shock, 4-day adult		12
heat shock, 4-day adult		12
Cadmium 50 mM 6 hrs, larvae L3		3
Cadmium 50 mM 12 hrs, larvae L3		6
Cadmium 50 mM 48 hrs, 4-day adult		11
Cadmium 100 mM 48 hrs, 4-day adult		31
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		49
Zinc 5 mM 12 hrs, larvae L3		6
Zinc 4.5 mM 48 hrs, 4-day adult		13
Ethanol 2.5% 3 hrs, larvae L3		24
Ethanol 5% 3 hrs, larvae L3		3
Ethanol 10% 3 hrs, larvae L3		4
Caffeine 1.5 mg/ml 4 hrs, larvae L3		4
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		29
Caffeine 25 mg/ml 48 hrs, 4-day adult		16
Paraquat 5 mM 48 hrs, 4-day adult		20
Paraquat 10 mM 48 hrs, 4-day adult		18
Rotenone 2 μg 12 hrs, larvae L3		3
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		15
cold shock, 4-day adult		12
heat shock, 4-day adult		12
Cadmium 50 mM 6 hrs, larvae L3		3
Cadmium 50 mM 12 hrs, larvae L3		6
Cadmium 50 mM 48 hrs, 4-day adult		11
Cadmium 100 mM 48 hrs, 4-day adult		31
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		49
Zinc 5 mM 12 hrs, larvae L3		6
Zinc 4.5 mM 48 hrs, 4-day adult		13
Ethanol 2.5% 3 hrs, larvae L3		24
Ethanol 5% 3 hrs, larvae L3		3
Ethanol 10% 3 hrs, larvae L3		4
Caffeine 1.5 mg/ml 4 hrs, larvae L3		4
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		29
Caffeine 25 mg/ml 48 hrs, 4-day adult		16
Paraquat 5 mM 48 hrs, 4-day adult		20
Paraquat 10 mM 48 hrs, 4-day adult		18
Rotenone 2 μg 12 hrs, larvae L3		3
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		15
cold shock, 4-day adult		12
heat shock, 4-day adult		12
Cadmium 50 mM 6 hrs, larvae L3		3
Cadmium 50 mM 12 hrs, larvae L3		6
Cadmium 50 mM 48 hrs, 4-day adult		11
Cadmium 100 mM 48 hrs, 4-day adult		31
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		(49)
Zinc 5 mM 12 hrs, larvae L3		6
Zinc 4.5 mM 48 hrs, 4-day adult		13
Ethanol 2.5% 3 hrs, larvae L3		24
Ethanol 5% 3 hrs, larvae L3		3
Ethanol 10% 3 hrs, larvae L3		4
Caffeine 1.5 mg/ml 4 hrs, larvae L3		4
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		29
Caffeine 25 mg/ml 48 hrs, 4-day adult		16
Paraquat 5 mM 48 hrs, 4-day adult		20
Paraquat 10 mM 48 hrs, 4-day adult		18
Rotenone 2 μg 12 hrs, larvae L3		3
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		15
cold shock, 4-day adult		12
heat shock, 4-day adult		12
Cadmium 50 mM 6 hrs, larvae L3		3
Cadmium 50 mM 12 hrs, larvae L3		6
Cadmium 50 mM 48 hrs, 4-day adult		11
Cadmium 100 mM 48 hrs, 4-day adult		31
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		49
Zinc 5 mM 12 hrs, larvae L3		6
Zinc 4.5 mM 48 hrs, 4-day adult		13
Ethanol 2.5% 3 hrs, larvae L3		24
Ethanol 5% 3 hrs, larvae L3		3
Ethanol 10% 3 hrs, larvae L3		4
Caffeine 1.5 mg/ml 4 hrs, larvae L3		4
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		29
Caffeine 25 mg/ml 48 hrs, 4-day adult		16
Paraquat 5 mM 48 hrs, 4-day adult		20
Paraquat 10 mM 48 hrs, 4-day adult		18
Rotenone 2 μg 12 hrs, larvae L3		3
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		15
cold shock, 4-day adult		12
heat shock, 4-day adult		12
Cadmium 50 mM 6 hrs, larvae L3		3
Cadmium 50 mM 12 hrs, larvae L3		6
Cadmium 50 mM 48 hrs, 4-day adult		11
Cadmium 100 mM 48 hrs, 4-day adult		31
Copper 0.5 mM 12 hrs, larvae L3		3
Copper 15 mM 48 hrs, 4-day adult		49
Zinc 5 mM 12 hrs, larvae L3		6
Zinc 4.5 mM 48 hrs, 4-day adult		13
Ethanol 2.5% 3 hrs, larvae L3		24
Ethanol 5% 3 hrs, larvae L3		3
Ethanol 10% 3 hrs, larvae L3		4
Caffeine 1.5 mg/ml 4 hrs, larvae L3		4
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		29
Caffeine 25 mg/ml 48 hrs, 4-day adult		16
Paraquat 5 mM 48 hrs, 4-day adult		20
Paraquat 10 mM 48 hrs, 4-day adult		18
Rotenone 2 μg 12 hrs, larvae L3		3
Rotenone 8 μg 12 hrs, larvae L3		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

External Data & Images

(Baumann et al., 1987, Tejedor et al., 1995)

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0259168

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG7835-RA

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal | pupal stage, with Scer\GAL4^pnr-MD237

mnb^GD12298

viable

mnb^EY14320

viable, with Scer\GAL4^pnr-MD237

mnb^GD13197

Sterility

Allele

fertile

mnb^EY14320

Other Phenotypes

Allele

behavior defective

visible, with Scer\GAL4^pnr-MD237

mnb^GD13197

Phenotype manifest in

Allele

embryonic/larval brain

trichogen cell, with Scer\GAL4^pnr-MD237

mnb^GD13197

Classical Alleles ( 19 )

For All Classical Alleles Show

Allele of mnb	Mutagen	Stocks	Known lesion
mnb^d10364	P-element activity	1	--
mnb^d11431	P-element activity	1	--
mnb^e02428	piggyBac transposase	1	--
mnb^e03885	piggyBac transposase	1	--
mnb^EY14320	P-element activity	1	--
mnb^f01371	piggyBac transposase	1	--
mnb^KG04573		1	--
mnb^NP6003		1	--
mnb^NP7209		1	--
mnb^UM-8014-3		1	--
mnb¹	ethyl methanesulfonate	0	Yes
mnb²	PM hybrid dysgenesis	0	Yes
mnb³	ethyl methanesulfonate	0	--
mnb⁴	X ray	0	Yes
mnb^f04483	piggyBac transposase	0	--
mnb^GG01512		0	--
mnb^GG01751		0	--
mnb^GG01814		0	--
mnb^UM-8181-3		0	--

Alleles Carried on Transgenic Constructs ( 8 )

For All Alleles Carried on Transgenic Constructs Show

Allele of mnb	Mutagen	Stocks	Known lesion
mnb^GD12298	in vitro construct - RNAi in vitro construct - regulatory fusion	2	Yes
mnb^GD13197	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
mnb^GL00104	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
mnb^HM05098	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
mnb^HMS01545	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
mnb^JF01364	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
mnb^KK102642	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
mnb^NIG.7826R	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes

Aneuploid Aberrations

Disrupted in

T(1;2)B27

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{EPgy2}mnb^EY14320

P{Mae-UAS.6.11}mnb^GG01512

P{Mae-UAS.6.11}mnb^GG01751

P{Mae-UAS.6.11}mnb^GG01814

P{XP}mnb^d10364

P{XP}mnb^d11431

miscellaneous insertions

P{}mnb²

P{RS3}mnb^UM-8014-3

P{RS3}mnb^UM-8181-3

P{SUPor-P}mnb^KG04573

insertion of enhancer trap binary system

P{GawB}mnb^NP6003

P{GawB}mnb^NP7209

Gene Ontology: Function, Process & Cellular Component ( 11 unique terms )

Terms Based on Experimental Evidence ( 5 terms )

Molecular Function ( 0 terms)

Biological Process

CV term

(Heisenberg and Wolf, 1984, Fischbach and Heisenberg, 1984)

References

brain development

inferred from mutant phenotype

circadian rhythm

inferred from genetic interaction with so

(Helfrich, 1986)

nervous system development

inferred from mutant phenotype

(Heisenberg et al., 1985)

olfactory learning

inferred from mutant phenotype

visual behavior

inferred from mutant phenotype

Cellular Component ( 0 terms)

Terms Based on Predictions or Assertions ( 7 terms )

Molecular Function

CV term

(FlyBase Curators et al., 2004-)

References

ATP binding

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290, InterPro:IPR017441

protein kinase activity

non-traceable author statement

(Morrison et al., 2000)

protein serine/threonine/tyrosine kinase activity

inferred from sequence or structural similarity with EMBL:X79769

(FlyBase, 1992-)

protein serine/threonine kinase activity

inferred from electronic annotation with InterPro:IPR008271

(FlyBase Curators et al., 2004-)

inferred from sequence or structural similarity

(Swiss-Prot Project Members, 1996.2.1)

non-traceable author statement

Biological Process

CV term

(Swiss-Prot Project Members, 1996.2.1)

References

brain development

non-traceable author statement

learning or memory

non-traceable author statement

(Sokolowski, 2001)

protein phosphorylation

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290, InterPro:IPR008271

(FlyBase Curators et al., 2004-)

non-traceable author statement

(Morrison et al., 2000)

Cellular Component ( 0 terms)

Sequence Ontology: Class of Gene

gene_with_edited_transcript

(Graveley et al., 2011)

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

protein-protein

Interacting group

Assay

References

mnb - CG14614

experimental knowledge based

(Guruharsha et al., 2011)

mnb - Reph

experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting

(Guruharsha et al., 2011, Mintseris et al., 2009.11.23)

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

mnb allele

Gene

References

External Data

BioGRID - A database of protein and genetic interactions

•

59093

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0259168

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

32771

SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores.

•

P49657

Orthologs

OrthoDB Orthologs (45) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG618C74

Dipteran inclusive ortholog search

EOG680J54

Insect inclusive ortholog search

EOG605QWF

Arthropod inclusive ortholog search

EOG6Z34W1

Metazoa inclusive ortholog search

EOG647TMG

Orthologs in Drosophila Species (EOG618C74)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA20611

Drosophila persimilis

Dper\GL14559

Drosophila willistoni

Dwil\GK10086

Drosophila virilis

Dvir\GJ15681

Drosophila mojavensis

Dmoj\GI16344

Drosophila grimshawi

Dgri\GH12565

Orthologs in non-Drosophila Dipterans (EOG680J54)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL003370

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL003400

Anopheles gambiae

Malaria mosquito

Agam\AGAP002119

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ010268

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ019411

Orthologs in non-Dipteran Insects (EOG605QWF)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\GB53062

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG003454

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH18766

Atta cephalotes

Leafcutter ant

Acep\ACEP13360

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO14576

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL12767

Linepithema humile

Argentine ant

Lhum\LH26139

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB27197

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI002557

Pediculus humanus

Human body louse

Phum\PHUM284180

Tribolium castaneum

Red flour beetle

Tcas\GLEAN_07717

Orthologs in non-Insect Arthropods (EOG6Z34W1)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\CD3D

Ixodes scapularis

Deer tick

Isca\ISCW022190

Orthologs in non-Arthropod Metazoa (EOG647TMG)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Caenorhabditis elegans

Nematode

Cele\mbk-1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_021153gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_019900gn

Danio rerio

Zebrafish

Drer\dyrk1ab

Danio rerio

Zebrafish

Drer\dyrk1aa

Danio rerio

Zebrafish

Drer\dyrk1b

Xenopus tropicalis

Western clawed frog

Xtro\dyrk1a

Xenopus tropicalis

Western clawed frog

Xtro\ENSXETG00000032961

Gallus gallus

Domestic chicken

Ggal\DYRK1A

Mus musculus

House mouse

Mmus\ENSMUSG00000002409

Mus musculus

House mouse

Mmus\Dyrk1a

Rattus norvegicus

Norway rat

Rnor\Dyrk1b

Rattus norvegicus

Norway rat

Rnor\Dyrk1a

Homo sapiens

Human

Hsap\DYRK1B

Homo sapiens

Human

Hsap\ENSG00000157540

Human Orthologs (2)

Gene

OMIM

HGNC

Hsap\DYRK1B

Hsap\ENSG00000157540

AAA Orthologs (11) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA20611

Drosophila persimilis

Dper\GL14559

Drosophila willistoni

Dwil\GK10086

Drosophila virilis

Dvir\GJ15681

Drosophila mojavensis

Dmoj\GI16344

Drosophila grimshawi

Dgri\GH12565

Stocks & Reagents

Stocks Listed in FlyBase ( 18 )

Bloomington

13526

y¹ P{SUPor-P}mnb^KG04573

28888

y¹ v¹; P{TRiP.HM05098}attP2

124281

w¹¹¹⁸ P{RS3}mnb^UM-8014-3

VDRC

v107066

P{KK102642}VIE-260B

v35283

w¹¹¹⁸; P{GD12298}v35283

v28628

w¹¹¹⁸; P{GD13197}v28628/TM3

v35282

w¹¹¹⁸; P{GD12298}v35282/TM3

More stocks available...

Genomic Clones ( 2 )

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 74 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

(Tejedor et al., 1995, Hong et al., 2012, Tejedor et al., 1995)

DRSC - Results from RNAi screens.

•

FBgn0259168

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

32771

Antibody Information

polyclonal

Other Information

Discoverer

M. Heisenberg.

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: mnb CG7835

(FlyBase Genome Annotators, 2008)

Additional comments

Annotations CG7826 and CG7835 merged as CG42273 in release 5.8 of the genome annotation.

(FlyBase Genome Annotators, 2008)

Apparently part of 'Sh complex'. Seems to be allelic to Sh, given map position and mnb-associated leg shaking (A. Ferrus and M. Heisenberg, unpublished); yet, Sh mutations do not lead to any grossly aberrant brain morphology and Sh⁵ plus Sh¹⁴ complement mnb¹ with regard to anatomical defects caused by the latter (M. Heisenberg, unpublished); mnb¹ also complements lethal alleles of 3 genes mapping just distally to Sh alleles (see Tanouye, Lam and Iverson, 1986), i.e., l(1)16Fa, l(1)16Fb and l(1)16Fc; an X-linked enhancer of mnb has been identified, which causes lethality when expressed along with the latter (M. Heisenberg, unpublished); the former maps near cv and (when in combination with mnb¹) leads to death late in pupation (mutants can be 'rescued' by opening the pupal case).

Other Comments

When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an increase in the proportion of S phase cells and/or aneuploidy, an increase in the proportion of G2/M phase cells, an increase in mitotic index and abnormal spindles are seen.

(Bettencourt-Dias et al., 2004)

Mutations in mnb cause an abnormal spacing of neuroblasts in the outer proliferation centre of the larval brain. As a consequence the larval brain exhibits a specific and marked reduction of the optic lobes and central brain hemispheres. The insufficient number of distinct neurons in the brain is correlated with specific abnormalities in visual and olfactory behaviour.