Gene Dmel\RhoGAP18B

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General Information

Symbol

Dmel\RhoGAP18B

Species

D. melanogaster

Name

RhoGAP18B

Annotation symbol

CG42274

Feature type

protein_coding_gene

FlyBase ID

FBgn0259169

Created / Updated

2003-12-01/2008-05-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

18A3-18A4

Sequence location

X:19,030,259..19,059,362 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol RhoGAP18B (CG42274, FBgn0259169). It has the cytological map location 18A3-18A4. Its sequence location is X:19030259..19059362. Its molecular function is described as GTPase activator activity. It is involved in the biological processes: actin filament organization; signal transduction. 23 alleles are reported. No phenotypic data is available. It has 4 annotated transcripts and 4 annotated polypeptides.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

18A3-18A4

Limits computationally determined from genome sequence between P{EP}CG7326^EP1133 and P{EP}CG7502^EP1326

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\RhoGAP18B for information on other features

Comments on Gene Model

Gene merge based on EST/cDNA data

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

RhoGAP18B-RB

FBtr0299622

2192

468

RhoGAP18B-RC

FBtr0299623

4446

1317

RhoGAP18B-RD

FBtr0299624

3928

752

RhoGAP18B-RF

FBtr0299625

1673

468

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

RhoGAP18B-PB

FBpp0288897

52.2

468

8.27

RhoGAP18B-PC

FBpp0288898

147.4

1317

6.87

RhoGAP18B-PD

FBpp0288899

84.5

752

4.85

RhoGAP18B-PF

FBpp0288900

52.2

468

8.27

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

RhoGAP (IPR000198)

Rho GTPase activation protein (IPR008936)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Maps to

Does NOT map to

Identified with

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal | recessive

RhoGAP18B^PG43

Phenotype manifest in

Allele

Classical Alleles ( 18 )

For All Classical Alleles Show

Allele of RhoGAP18B	Mutagen	Stocks	Known lesion
RhoGAP18B^EP1326	P-element activity	2	--
RhoGAP18B^EP1439	P-element activity	1	--
RhoGAP18B^EP1621	P-element activity	1	--
RhoGAP18B^GS9097		0	--
RhoGAP18B^KG00160	P-element activity	1	--
RhoGAP18B^PG43	Δ2-3	0	--
RhoGAP18B^d01002	P-element activity	1	--
RhoGAP18B^d02256	P-element activity	0	--
RhoGAP18B^d05271	P-element activity	1	--
RhoGAP18B^d07621	P-element activity	0	--
RhoGAP18B^d08872	P-element activity	1	--
RhoGAP18B^d09463	P-element activity	0	--
RhoGAP18B^d09663	P-element activity	0	--
RhoGAP18B^e02324	piggyBac transposase	1	--
RhoGAP18B^e02346	piggyBac transposase	1	--
RhoGAP18B^f01190	piggyBac transposase	1	--
RhoGAP18B^f03742	piggyBac transposase	1	--
RhoGAP18B^f06094	piggyBac transposase	1	--

Alleles Carried on Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Allele of RhoGAP18B	Mutagen	Stocks	Known lesion
RhoGAP18B^GD12250	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
RhoGAP18B^GD12256	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
RhoGAP18B^GD12266	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
RhoGAP18B^GD15353	in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
RhoGAP18B^{dsRNA.Scer\UAS}	in vitro construct \| regulatory fusion in vitro construct \| RNAi	2	Yes

Aneuploid Aberrations

Useful deficiency

Df(1)22622DL

Useful duplication

Dp(1;1)B^STAG

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD12250}

P{GD12266}

P{GD15353}

P{UAS-RhoGAP18B-dsRNA}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{RB}RhoGAP18B^e02324

PBac{RB}RhoGAP18B^e02346

PBac{WH}RhoGAP18B^f01190

PBac{WH}RhoGAP18B^f03742

PBac{WH}RhoGAP18B^f06094

P{SUPor-P}RhoGAP18B^KG00160

insertion of enhancer trap binary system

P{GawB}RhoGAP18B^PG43

insertion of mobile activating element

P{EP}RhoGAP18B^EP1326

P{EP}RhoGAP18B^EP1439

P{EP}RhoGAP18B^EP1621

P{GSV6}RhoGAP18B^GS9097

P{XP}RhoGAP18B^d01002

P{XP}RhoGAP18B^d02256

P{XP}RhoGAP18B^d05271

P{XP}RhoGAP18B^d07621

P{XP}RhoGAP18B^d08872

P{XP}RhoGAP18B^d09463

P{XP}RhoGAP18B^d09663

Related Comments

Please look at the allele reports for the complete phenotype data

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R⁺ cells: increased or polarized (uneven) accumulation of F-actin.

(Kiger et al., 2003)

Gene Ontology: Function, Process & Cellular Component ( 4 )

Molecular Function

CV term

Evidence

References

GTPase activator activity

inferred from sequence or structural similarity with NCBI_NP:064656

(FlyBase Genome Annotators, 2002-2003)

Biological Process

CV term

Evidence

References

actin filament organization

inferred from mutant phenotype

(Kiger et al., 2003)

signal transduction

inferred from electronic annotation with InterPro:IPR000198, InterPro:IPR008936

(FlyBase Curators et al., 2004-)

Cellular Component

CV term

Evidence

References

intracellular

inferred from electronic annotation with InterPro:IPR000198, InterPro:IPR008936

(FlyBase Curators et al., 2004-)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

RhoGAP18B allele

Gene

References

External Data

Linkouts

Orthologs

Genome-wide drosophilid orthologs

Dgri\GH24224

Dper\GL14654

Dper\GL14655

Dpse\GA20396

Dpse\GA22300

Curated drosophilid orthologs

Dpse\GA20383

Dpse\GA20396

Linkouts

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 22 )

Harvard

P{XP}RhoGAP18B^d01002

P{XP}RhoGAP18B^d05271

P{XP}RhoGAP18B^d08872

PBac{RB}RhoGAP18B^e02324

PBac{RB}RhoGAP18B^e02346

PBac{WH}RhoGAP18B^f01190

PBac{WH}RhoGAP18B^f03742

PBac{WH}RhoGAP18B^f06094

Szeged

P{EP}RhoGAP18B^EP1326

P{EP}RhoGAP18B^EP1621

P{EP}RhoGAP18B^EP1439

VDRC

v28047

w¹¹¹⁸; P{GD12256}v28047

More stocks available...

Genomic Clones ( 1 )

BACR49A05

cDNA Clones ( 23 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones