Gene Dmel\rn

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General Information

Symbol

Dmel\rn

Species

D. melanogaster

Name

rotund

Annotation symbol

CG42277

Feature type

protein_coding_gene

FlyBase ID

FBgn0259172

Gene Model Status

Current

Stock availability

112 publicly available

Also Known As

roe, CG10040, P5.3

Genomic Location

Chromosome (arm)

Recombination map

3-47.6

Cytogenetic map

84D3-84D3

Sequence location

3R:3,099,460..3,135,119 [-]

Genomic Maps

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modENCODE GBrowse

Summary Information

Automatically generated summary

See sections below for more information

The gene rotund is referred to in FlyBase by the symbol Dmel\rn (CG42277, FBgn0259172). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: sequence-specific distal enhancer binding RNA polymerase II transcription factor activity. There is experimental evidence that it is involved in the biological process: compound eye development; negative regulation of transcription from RNA polymerase II promoter; imaginal disc-derived leg morphogenesis; negative regulation of transcription, DNA-dependent. 73 alleles are reported. The phenotypes of these alleles are annotated with: organ system subdivision; adult segment; organ system; external compound sense organ; thoracic segment; appendage segment; imaginal precursor; epithelial furrow; metatarsus; peripheral nervous system. It has 3 annotated transcripts and 3 annotated polypeptides. Protein features are: Zinc finger, C2H2; Zinc finger, C2H2-like; Zinc finger, C2H2-type/integrase, DNA-binding. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of low expression to a trough of no expression detected. Peak expression observed within 12-24 hour embryonic stages, during late larval stages, at stages throughout the pupal period, in adult male stages. Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. Gene sequence location is 3R:3099460..3135119.

External Summaries

Phenotypic Description from the Red Book (Lindsley & Zimm 1992)

Gene/Allele symbols may differ from current usage

rn: rotund (S. Kerridge)

rn: rotund
From Bridges and Brehme, 1944, Carnegie Inst. Washington Publ. No. 552: 159.

All alleles are viable as homozygotes. Both males and females are sterile; sterility is not germ line dependent. Two transcripts from the rotund region of Drosophila show similar positional specificities in imaginal disc tissues. Adult defects are restricted to homologous distal parts of the appendages, i.e., the antennae, legs, wings, halteres, and proboscis. In the antenna the basal capsule is missing and the third antennal segment is reduced in size; all other antennal and eye disc derivatives are normal. In all three pairs of legs abnormalities caused by the lack of rn+ product are localized specifically in the tarsus; instead of five individual tarsal segments, a single tarsus-like segment differentiates. The distal claw and proximal leg articulations (tibia, femur, trochanter, and coxa) are unaffected. Incomplete and duplicated joints seen at the presumptive positions of the tarsus 1-2 and 4-5 joints; intermediate joints virtually absent (Held, Duarte, and Derakhshanian, 1986, Wilhelm Roux's Arch. Dev Biol. 195: 145-57). Of the wing disc derivatives, the medial and distal costa are fused and the corresponding region in the posterior wing, the alula, is smaller, making the wing as a whole appear shorter; distal wing and mesonotum are formed as in wild type. Vein L5 and to a lesser extent L2 interrupted (rn3). Specific and localized deficiencies of labial and haltere disc derivatives are also evident. Genital and abdominal patterns are indistinguishable from wild-type patterns; however, seminal receptacles shorter than in wild-type females. Examination of the imaginal discs from third-instar rn larvae shows localized cell death in regions determined from fate maps to give rise to distal appendage parts (Cavallin).

rnD: rotund-Dominant

Dominant mutation with complete penetrance and high expressivity. In Dipr/+ flies the distal wing blade reduced to two-thirds normal length; triple row replaced by two to three rows of irregularly sized bristles resembling those found on costa; occasionally these bristles may be bracted as are those of proximal costa. Posterior row of marginal bristles absent distally. Venation in proximal portion of wing irregular and crowded but more nearly normal distally. Trichomes of wing blade organized into whorls. Capitellum of haltere covered with small adventitious bristles and sometimes pedicellar-like sensilla. Pleura, coxa, trochanter, femur, tibia, and fifth tarsal segment unaffected; other tarsal segments reduced; tarsal segments two to four variably fused; ectopic sex combs form on second tarsal segment in 70-90% of forelegs. Basal cylinder of antenna reduced or missing; replaced by bristle elements at third-segment-arista junction resembling those on third segment. Proboscis contains five to seven rows of pseudotracheae rather than normal twelve; replaced by bristles laterally. Kerridge suggests transformation of distal into more proximal appendage elements. Homozygotes survive and are more extreme.

roe: roughened eye

Produces a slight roughening of the eye owing to the irregular disposition of the facets; interommatidial bristles lost from posterior portion of the eye. Extreme expression in mutant/deficiency heterozygotes and more so in deficiency homozygotes; enhanced roughening and posterior loss of ommatidial bristles; in addition the eye is reduced in size and remaining interommatidial bristles are clumped. RK1.

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2011_10

FB2012_01

References

Herz et al., 2010, Mol. Cell. Biol. 30(10): 2485--2497

Controlled Vocabulary Terms

negative regulation of transcription from RNA polymerase II promoter

sequence-specific distal enhancer binding RNA polymerase II transcription factor activity

Sequence features

HFA12546

BKN22555

HFA12500

BKN45813

HFA12543

BKN30545

HFA12544

All updates

Click here to see a list of all updates to this record from FB2010_08 and on.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

84D3-84D3

Limits computationally determined from genome sequence between P{EP}EP3060^EP3060 and P{PZ}grn⁰⁵⁹³⁰

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

84D-84E

(determined by in situ hybridisation)

(Shilkova et al., 2000)

84D3-84D4

(determined by in situ hybridisation)

(Agnel et al., 1989)

Experimentally Determined Recombination Data

Location

3-47.6

(Puro and Nygren, 1975)

3-47.7 +/- 1.1

(Carlson, 1956)

Left of (cM)

Right of (cM)

Antp

(Lewis et al., 1980)

Notes

Mapping based on rn³.

(Puro and Nygren, 1975)

Gene Model & Products

Please see the GBrowse view of Dmel\rn for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.43

Gene merge based on EST/cDNA data.

Gene model includes transcripts encoding non-overlapping portions of the full CDS.

Due to an oversight in the implementation of gene annotation merges, the symbols for some of the transcripts and polypeptides of this gene were changed to non-unique designations in r5.8. Although there were no conflicts within a given release, the same symbols were used for different isoforms in different releases. The affected gene product symbols have now been changed to symbols not used previously in the database; see individual transcript reports for details.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

rn-RC

FBtr0299637

NM_206456

2444

692

rn-RE

FBtr0300518

NM_141458

3661

946

rn-RF

FBtr0299639

NM_001144549

3149

927

Additional Transcript Data & Comments

Reported size (kB)

5.3, 1.7 (northern blot)

(Agnel et al., 1989)

1.7 (unknown)

(Agnel et al., 1992)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

rn-PC

FBpp0288912

75.8

692

9.40

NP_996178

AAS65102

rn-PE

FBpp0289745

100.9

946

8.24

NP_649715

AAF54032

rn-PF

FBpp0288914

99.6

927

8.23

NP_001138021

ACL83480

Additional Polypeptide Data & Comments

Reported size (kDa)

384 (aa); 44 (kD)

(Agnel et al., 1992)

384, 383 (aa)

(Hoemann et al., 1996)

Comments

It is unclear which ATG is used for translation initiation. If the downstream ATG is the actual initation site, the protein is 362aa and 41kD.

form 1

form 2

External Data

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Zinc finger, C2H2-type/integrase, DNA-binding (IPR013087)

Zinc finger, C2H2-like (IPR015880)

Zinc finger, C2H2 (IPR007087)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

pupal stage | early

imaginal disc

(Agnel et al., 1992)

pupal stage & spermatogenesis & adult stage

spermatocyte

(Agnel et al., 1992)

spermatogenesis & adult stage

testis

(Agnel et al., 1992, Hoemann et al., 1996)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

prepupal stage P1 -- P2

adepithelial cell of dorsal mesothoracic disc

(Agnel et al., 1989)