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Gene Dmel\Nedd4

General Information
Symbol	Dmel\Nedd4	Species	D. melanogaster
Name	Nedd4	Annotation symbol	CG42279
Feature type	protein_coding_gene	FlyBase ID	FBgn0259174
Gene Model Status	Current	Stock availability	18 publicly available
Also Known As	DNedd4, CG7555
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	74D3-74D3	Sequence location	3L:17,523,181..17,539,760 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Nedd4 is referred to in FlyBase by the symbol Dmel\Nedd4 (CG42279, FBgn0259174). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: Notch binding; protein binding; protein domain specific binding. There is experimental evidence that it is involved in the biological process: negative regulation of Notch signaling pathway; receptor internalization; positive regulation of receptor internalization; axon guidance; positive regulation of synapse assembly; neuromuscular junction development; positive regulation of endocytosis; axon midline choice point recognition; protein ubiquitination. 53 alleles are reported. The phenotypes of these alleles are annotated with: organ system subdivision; organ system; adult segment; thoracic segment; adult; region of integument; SNb; non-connected developing system; embryonic/larval central nervous system; multicellular structure. It has 9 annotated transcripts and 9 annotated polypeptides. Protein features are: C2 calcium-dependent membrane targeting; C2 calcium/lipid-binding domain, CaLB; C2 domain; C2 membrane targeting protein; E3 ubiquitin-protein ligase, SMURF1 type; HECT; WW domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, larval/adult salivary gland, adult spermathecae, adult male accessory gland. Gene sequence location is 3L:17523181..17539760.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02	Controlled Vocabulary Terms nucleus protein ubiquitination involved in ubiquitin-dependent protein catabolic process
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 74D3-74D3   Limits computationally determined from genome sequence between P{PZ}frc00073&P{PZ}frc02619 and P{lacW}l(3)j11B2j11B2
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Nedd4 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.45 Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated Gene merge based on EST/cDNA data Low-frequency RNA-seq exon junction(s) not annotated. Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters. Due to an oversight in the implementation of gene annotation merges, the symbols for some of the transcripts and polypeptides of this gene were changed to non-unique designations in r5.8. Although there were no conflicts within a given release, the same symbols were used for different isoforms in different releases. The affected gene product symbols have now been changed to symbols not used previously in the database; see individual transcript reports for details.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Nedd4-RE FBtr0299642  NM_206394   3384   793 Nedd4-RF FBtr0299645  NM_168737   3917   956 Nedd4-RG FBtr0299646  NM_168738   3942   931 Nedd4-RH FBtr0299648  NM_001144493   4131   994 Nedd4-RI FBtr0299647  NM_001144492   4098   983 Nedd4-RJ FBtr0300519  NM_140736   4454   1007 Nedd4-RK FBtr0300520  NM_168736   3651   834 Nedd4-RL FBtr0331843  NM_001275061   4164   1005 Nedd4-RM FBtr0331844  NM_001275062   4146   999
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Nedd4-PE   FBpp0288917   91.2   793   5.33     NP_996116   AAS64973 Nedd4-PF   FBpp0288920   109.5   956   5.93     NP_730283   AAN11695 Nedd4-PG   FBpp0288921   106.2   931   5.02     NP_730284   AAN11696 Nedd4-PH   FBpp0288923   113.4   994   6.47     NP_001137965   ACL83320 Nedd4-PI   FBpp0288922   111.9   983   6.40     NP_001137964   ACL83319 Nedd4-PJ   FBpp0289746   114.9   1007   6.57     NP_648993   AAF49328 Nedd4-PK   FBpp0289747   95.3   834   6.15     NP_730282   AAN11694 Nedd4-PL   FBpp0304227   114.6   1005   6.57     NP_001261990   AGB94683 Nedd4-PM   FBpp0304228   113.6   999   6.44     NP_001261991   AGB94684
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • C2 membrane targeting protein (IPR018029) C2 calcium-dependent membrane targeting (IPR000008) HECT (IPR000569) WW domain (IPR001202) C2 calcium/lipid-binding domain, CaLB (IPR008973) C2 domain (IPR020477) E3 ubiquitin-protein ligase, SMURF1 type (IPR024928)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA140889   AA140890   AA697737   AA978552   AE014296 AAF49328   AE014296 AAN11694   AE014296 AAN11695   AE014296 AAN11696   AE014296 AAS64973   AE014296 ACL83319   AE014296 ACL83320   AE014296 AGB94683   AE014296 AGB94684   AI293466   AI295031   AI455849   AI516201   AI519779   AI533013   AI945962   AJ278468 CAC42101   AW944376   AY058619 AAL13848   AY061595 AAL29143   BF494281   BF495288   BG636519   BG639374   BI161516   BI161517   BI162612   BI163264   BI165311   BI165932   BI166598   BI167314   BI169120   BI172695   BI229357   BI232409   BI237644   BI240541   BI356058   BI363327   BI363751   BI364338   BI364908   BI365923   BI366048   BI369269   BI370824   BI483624   BI486524   BP544069   BP548957   BP557486   BP559993   BT025907   BT072825   BZ748761   CO154647   CO155249   CO155647   CO157096   CO178308   CO188597   CO262672   CO264133   CO268580   CO268938   CO271156   CO272220   CO280429   CO283104   CO284562   CO299465   CO300739   CO316389   CO316862   CO319862   CO321902   CO322155   CO322883   CO323361   CO324823   CO327103   CO327119   CO343218   CO343946   CZ466790   CZ466791   CZ466810   CZ466847   CZ466854   CZ474435   CZ483206   CZ486049   CZ486050   CZ487553   EC057566   EC062470   EC063037   EC063387   EC064801   EC069275   EC069298   EC069949   EC071592   EC079972   EC083272   EC197159   EC197195   EC203728   EC204469   EC204609   EC207223   EC213117   EC215303   EC215969   EC217059   EC219448   EC223478   EC226897   EC228808   EC234091   EC234172   EC234952   EC235086   EC243047   EC243434   EC245203   EC251339   EC251912   EC252515   EC258465   EC261932   EC262901   EC267701   EL872980   EL872981   EL873072   EL873073   EL873157   EL873158   EV582178   EV585198   EV595334   GH948163   GH948260   GH948507   GH954819   GH955496   GH958742   GH959593   GH965815   GH972391   GH973181   GH981203   GH981247   GH982117   GH984512
UniProtKB/Swiss-Prot	Q9VVI3
UniProtKB/TrEMBL	Q8IQR5 B7Z068 B7Z067
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 13 -- 16   organism | ubiquitous   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
cytoplasm inferred from direct assay (Ing et al., 2007, Zhong et al., 2011) inferred from direct assay (assigned by UniProtKB) (Sakata et al., 2004) plasma membrane inferred from direct assay (Zhong et al., 2011)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, larval/adult salivary gland, adult spermathecae, adult male accessory gland. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    28 central nervous system, larvae L3    19 central nervous system, pupae P8    17 head, virgin 1-day female    31 head, virgin 4-day female    25 head, virgin 20-day female    20 head, mated 1-day female    28 head, mated 4-day female    25 head, mated 20-day female    19 head, mated 1-day male    38 head, mated 4-day male    38 head, mated 20-day male    37 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    27 digestive system, larvae L3 wandering    40 digestive system, 1-day adult    25 digestive system, 4-day adult    31 digestive system, 20-day adult    28 fat body, larvae L3 wandering    4 fat body, white prepupae    11 fat body, pupae P8    9 carcass, larvae L3 wandering    15 carcass, 1-day adult    12 carcass, 4-day adult    15 carcass, 20-day adult    13 ovary, virgin 4-day female    89 ovary, mated 4-day female    72 testis, mated 4-day male    18 accessory gland, mated 4-day male    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (28) central nervous system, larvae L3    19 central nervous system, pupae P8    17 head, virgin 1-day female  (31) head, virgin 4-day female    25 head, virgin 20-day female    20 head, mated 1-day female  (28) head, mated 4-day female    25 head, mated 20-day female    19 head, mated 1-day male  (38) head, mated 4-day male  (38) head, mated 20-day male  (37) salivary gland, larvae L3 wandering    8 salivary gland, white prepupae  (27) digestive system, larvae L3 wandering  (40) digestive system, 1-day adult    25 digestive system, 4-day adult  (31) digestive system, 20-day adult  (28) fat body, larvae L3 wandering    4 fat body, white prepupae    11 fat body, pupae P8    9 carcass, larvae L3 wandering    15 carcass, 1-day adult    12 carcass, 4-day adult    15 carcass, 20-day adult    13 ovary, virgin 4-day female  (89) ovary, mated 4-day female  (72) testis, mated 4-day male    18 accessory gland, mated 4-day male    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    28 central nervous system, larvae L3    19 central nervous system, pupae P8    17 head, virgin 1-day female    31 head, virgin 4-day female    25 head, virgin 20-day female    20 head, mated 1-day female    28 head, mated 4-day female    25 head, mated 20-day female    19 head, mated 1-day male    38 head, mated 4-day male    38 head, mated 20-day male    37 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    27 digestive system, larvae L3 wandering    40 digestive system, 1-day adult    25 digestive system, 4-day adult    31 digestive system, 20-day adult    28 fat body, larvae L3 wandering    4 fat body, white prepupae    11 fat body, pupae P8    9 carcass, larvae L3 wandering    15 carcass, 1-day adult    12 carcass, 4-day adult    15 carcass, 20-day adult    13 ovary, virgin 4-day female    89 ovary, mated 4-day female    72 testis, mated 4-day male    18 accessory gland, mated 4-day male    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    28 central nervous system, larvae L3    19 central nervous system, pupae P8    17 head, virgin 1-day female    31 head, virgin 4-day female    25 head, virgin 20-day female    20 head, mated 1-day female    28 head, mated 4-day female    25 head, mated 20-day female    19 head, mated 1-day male    38 head, mated 4-day male    38 head, mated 20-day male    37 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    27 digestive system, larvae L3 wandering    40 digestive system, 1-day adult    25 digestive system, 4-day adult    31 digestive system, 20-day adult    28 fat body, larvae L3 wandering    4 fat body, white prepupae    11 fat body, pupae P8    9 carcass, larvae L3 wandering    15 carcass, 1-day adult    12 carcass, 4-day adult    15 carcass, 20-day adult    13 ovary, virgin 4-day female    89 ovary, mated 4-day female    72 testis, mated 4-day male    18 accessory gland, mated 4-day male    10 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    28 central nervous system, larvae L3    19 central nervous system, pupae P8    17 head, virgin 1-day female    31 head, virgin 4-day female    25 head, virgin 20-day female    20 head, mated 1-day female    28 head, mated 4-day female    25 head, mated 20-day female    19 head, mated 1-day male    38 head, mated 4-day male    38 head, mated 20-day male    37 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    27 digestive system, larvae L3 wandering    40 digestive system, 1-day adult    25 digestive system, 4-day adult    31 digestive system, 20-day adult    28 fat body, larvae L3 wandering    4 fat body, white prepupae    11 fat body, pupae P8    9 carcass, larvae L3 wandering    15 carcass, 1-day adult    12 carcass, 4-day adult    15 carcass, 20-day adult    13 ovary, virgin 4-day female    89 ovary, mated 4-day female    72 testis, mated 4-day male    18 accessory gland, mated 4-day male    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  28 central nervous system, larvae L3    19 central nervous system, pupae P8    17 head, virgin 1-day female  31 head, virgin 4-day female    25 head, virgin 20-day female    20 head, mated 1-day female  28 head, mated 4-day female    25 head, mated 20-day female    19 head, mated 1-day male  (38) head, mated 4-day male  (38) head, mated 20-day male  (37) salivary gland, larvae L3 wandering    8 salivary gland, white prepupae  27 digestive system, larvae L3 wandering  (40) digestive system, 1-day adult    25 digestive system, 4-day adult  31 digestive system, 20-day adult  28 fat body, larvae L3 wandering    4 fat body, white prepupae    11 fat body, pupae P8    9 carcass, larvae L3 wandering    15 carcass, 1-day adult    12 carcass, 4-day adult    15 carcass, 20-day adult    13 ovary, virgin 4-day female  (89) ovary, mated 4-day female  (72) testis, mated 4-day male    18 accessory gland, mated 4-day male    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    28 central nervous system, larvae L3    19 central nervous system, pupae P8    17 head, virgin 1-day female    31 head, virgin 4-day female    25 head, virgin 20-day female    20 head, mated 1-day female    28 head, mated 4-day female    25 head, mated 20-day female    19 head, mated 1-day male    38 head, mated 4-day male    38 head, mated 20-day male    37 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    27 digestive system, larvae L3 wandering    40 digestive system, 1-day adult    25 digestive system, 4-day adult    31 digestive system, 20-day adult    28 fat body, larvae L3 wandering    4 fat body, white prepupae    11 fat body, pupae P8    9 carcass, larvae L3 wandering    15 carcass, 1-day adult    12 carcass, 4-day adult    15 carcass, 20-day adult    13 ovary, virgin 4-day female    89 ovary, mated 4-day female    72 testis, mated 4-day male    18 accessory gland, mated 4-day male    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    28 central nervous system, larvae L3    19 central nervous system, pupae P8    17 head, virgin 1-day female    31 head, virgin 4-day female    25 head, virgin 20-day female    20 head, mated 1-day female    28 head, mated 4-day female    25 head, mated 20-day female    19 head, mated 1-day male    38 head, mated 4-day male    38 head, mated 20-day male    37 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    27 digestive system, larvae L3 wandering    40 digestive system, 1-day adult    25 digestive system, 4-day adult    31 digestive system, 20-day adult    28 fat body, larvae L3 wandering    4 fat body, white prepupae    11 fat body, pupae P8    9 carcass, larvae L3 wandering    15 carcass, 1-day adult    12 carcass, 4-day adult    15 carcass, 20-day adult    13 ovary, virgin 4-day female    89 ovary, mated 4-day female    72 testis, mated 4-day male    18 accessory gland, mated 4-day male    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    67 embryo 02-04hr    28 embryo 04-06hr    24 embryo 06-08hr    20 embryo 08-10hr    20 embryo 10-12hr    21 embryo 12-14hr    17 embryo 14-16hr    14 embryo 16-18hr    19 embryo 18-20hr    21 embryo 20-22hr    23 embryo 22-24hr    26 larva L1    16 larva L2    14 larva L3 12hr old    13 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    23 white prepupae new    30 white prepupae 12hr    36 white prepupae 24hr    26 pupae 2d postWPP    22 pupae 3d postWPP    22 pupae 4d postWPP    22 adult male 01day    24 adult male 05day    26 adult male 30day    25 adult female 01day    29 adult female 05day    45 adult female 30day    46 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (67) embryo 02-04hr  (28) embryo 04-06hr    24 embryo 06-08hr    20 embryo 08-10hr    20 embryo 10-12hr    21 embryo 12-14hr    17 embryo 14-16hr    14 embryo 16-18hr    19 embryo 18-20hr    21 embryo 20-22hr    23 embryo 22-24hr  26 larva L1    16 larva L2    14 larva L3 12hr old    13 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    23 white prepupae new  (30) white prepupae 12hr  (36) white prepupae 24hr  26 pupae 2d postWPP    22 pupae 3d postWPP    22 pupae 4d postWPP    22 adult male 01day    24 adult male 05day  26 adult male 30day    25 adult female 01day  (29) adult female 05day  (45) adult female 30day  (46) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    67 embryo 02-04hr    28 embryo 04-06hr    24 embryo 06-08hr    20 embryo 08-10hr    20 embryo 10-12hr    21 embryo 12-14hr    17 embryo 14-16hr    14 embryo 16-18hr    19 embryo 18-20hr    21 embryo 20-22hr    23 embryo 22-24hr    26 larva L1    16 larva L2    14 larva L3 12hr old    13 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    23 white prepupae new    30 white prepupae 12hr    36 white prepupae 24hr    26 pupae 2d postWPP    22 pupae 3d postWPP    22 pupae 4d postWPP    22 adult male 01day    24 adult male 05day    26 adult male 30day    25 adult female 01day    29 adult female 05day    45 adult female 30day    46 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    67 embryo 02-04hr    28 embryo 04-06hr    24 embryo 06-08hr    20 embryo 08-10hr    20 embryo 10-12hr    21 embryo 12-14hr    17 embryo 14-16hr    14 embryo 16-18hr    19 embryo 18-20hr    21 embryo 20-22hr    23 embryo 22-24hr    26 larva L1    16 larva L2    14 larva L3 12hr old    13 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    23 white prepupae new    30 white prepupae 12hr    36 white prepupae 24hr    26 pupae 2d postWPP    22 pupae 3d postWPP    22 pupae 4d postWPP    22 adult male 01day    24 adult male 05day    26 adult male 30day    25 adult female 01day    29 adult female 05day    45 adult female 30day    46 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    67 embryo 02-04hr    28 embryo 04-06hr    24 embryo 06-08hr    20 embryo 08-10hr    20 embryo 10-12hr    21 embryo 12-14hr    17 embryo 14-16hr    14 embryo 16-18hr    19 embryo 18-20hr    21 embryo 20-22hr    23 embryo 22-24hr    26 larva L1    16 larva L2    14 larva L3 12hr old    13 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    23 white prepupae new    30 white prepupae 12hr    36 white prepupae 24hr    26 pupae 2d postWPP    22 pupae 3d postWPP    22 pupae 4d postWPP    22 adult male 01day    24 adult male 05day    26 adult male 30day    25 adult female 01day    29 adult female 05day    45 adult female 30day    46 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (67) embryo 02-04hr  28 embryo 04-06hr    24 embryo 06-08hr    20 embryo 08-10hr    20 embryo 10-12hr    21 embryo 12-14hr    17 embryo 14-16hr    14 embryo 16-18hr    19 embryo 18-20hr    21 embryo 20-22hr    23 embryo 22-24hr  26 larva L1    16 larva L2    14 larva L3 12hr old    13 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    23 white prepupae new  30 white prepupae 12hr  (36) white prepupae 24hr  26 pupae 2d postWPP    22 pupae 3d postWPP    22 pupae 4d postWPP    22 adult male 01day    24 adult male 05day  26 adult male 30day    25 adult female 01day  29 adult female 05day  (45) adult female 30day  (46) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    67 embryo 02-04hr    28 embryo 04-06hr    24 embryo 06-08hr    20 embryo 08-10hr    20 embryo 10-12hr    21 embryo 12-14hr    17 embryo 14-16hr    14 embryo 16-18hr    19 embryo 18-20hr    21 embryo 20-22hr    23 embryo 22-24hr    26 larva L1    16 larva L2    14 larva L3 12hr old    13 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    23 white prepupae new    30 white prepupae 12hr    36 white prepupae 24hr    26 pupae 2d postWPP    22 pupae 3d postWPP    22 pupae 4d postWPP    22 adult male 01day    24 adult male 05day    26 adult male 30day    25 adult female 01day    29 adult female 05day    45 adult female 30day    46 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    67 embryo 02-04hr    28 embryo 04-06hr    24 embryo 06-08hr    20 embryo 08-10hr    20 embryo 10-12hr    21 embryo 12-14hr    17 embryo 14-16hr    14 embryo 16-18hr    19 embryo 18-20hr    21 embryo 20-22hr    23 embryo 22-24hr    26 larva L1    16 larva L2    14 larva L3 12hr old    13 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    23 white prepupae new    30 white prepupae 12hr    36 white prepupae 24hr    26 pupae 2d postWPP    22 pupae 3d postWPP    22 pupae 4d postWPP    22 adult male 01day    24 adult male 05day    26 adult male 30day    25 adult female 01day    29 adult female 05day    45 adult female 30day    46 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    56 Schneider line 2 Sg4    55 embryonic 1182-4H    32 embryonic GM2    41 embryonic Kc167    60 embryonic S1    23 embryonic S3    15 leg disc CME L1    40 wing disc CME-W2    34 wing disc ML-DmD8    29 wing disc ML-DmD9    30 wing disc ML-DmD16-c3    27 wing disc ML-DmD21    25 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    31 eye-antennal disc ML-DmD11    18 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    41 CNS ML-DmBG1-c1    21 CNS ML-DmBG2-c2    29 tumorous blood cells mbn2    42 ovary fGS/OSS    27 ovary OSC    17 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (56) Schneider line 2 Sg4  (55) embryonic 1182-4H  (32) embryonic GM2  (41) embryonic Kc167  (60) embryonic S1    23 embryonic S3    15 leg disc CME L1  (40) wing disc CME-W2  (34) wing disc ML-DmD8  (29) wing disc ML-DmD9  (30) wing disc ML-DmD16-c3  (27) wing disc ML-DmD21    25 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3  (31) eye-antennal disc ML-DmD11    18 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1  (41) CNS ML-DmBG1-c1    21 CNS ML-DmBG2-c2  (29) tumorous blood cells mbn2  (42) ovary fGS/OSS  (27) ovary OSC    17 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    56 Schneider line 2 Sg4    55 embryonic 1182-4H    32 embryonic GM2    41 embryonic Kc167    60 embryonic S1    23 embryonic S3    15 leg disc CME L1    40 wing disc CME-W2    34 wing disc ML-DmD8    29 wing disc ML-DmD9    30 wing disc ML-DmD16-c3    27 wing disc ML-DmD21    25 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    31 eye-antennal disc ML-DmD11    18 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    41 CNS ML-DmBG1-c1    21 CNS ML-DmBG2-c2    29 tumorous blood cells mbn2    42 ovary fGS/OSS    27 ovary OSC    17 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    56 Schneider line 2 Sg4    55 embryonic 1182-4H    32 embryonic GM2    41 embryonic Kc167    60 embryonic S1    23 embryonic S3    15 leg disc CME L1    40 wing disc CME-W2    34 wing disc ML-DmD8    29 wing disc ML-DmD9    30 wing disc ML-DmD16-c3    27 wing disc ML-DmD21    25 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    31 eye-antennal disc ML-DmD11    18 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    41 CNS ML-DmBG1-c1    21 CNS ML-DmBG2-c2    29 tumorous blood cells mbn2    42 ovary fGS/OSS    27 ovary OSC    17 ovary OSS    13 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    56 Schneider line 2 Sg4    55 embryonic 1182-4H    32 embryonic GM2    41 embryonic Kc167    60 embryonic S1    23 embryonic S3    15 leg disc CME L1    40 wing disc CME-W2    34 wing disc ML-DmD8    29 wing disc ML-DmD9    30 wing disc ML-DmD16-c3    27 wing disc ML-DmD21    25 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    31 eye-antennal disc ML-DmD11    18 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    41 CNS ML-DmBG1-c1    21 CNS ML-DmBG2-c2    29 tumorous blood cells mbn2    42 ovary fGS/OSS    27 ovary OSC    17 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (56) Schneider line 2 Sg4  (55) embryonic 1182-4H  32 embryonic GM2  (41) embryonic Kc167  (60) embryonic S1    23 embryonic S3    15 leg disc CME L1  (40) wing disc CME-W2  (34) wing disc ML-DmD8  29 wing disc ML-DmD9  30 wing disc ML-DmD16-c3  27 wing disc ML-DmD21    25 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3  31 eye-antennal disc ML-DmD11    18 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1  (41) CNS ML-DmBG1-c1    21 CNS ML-DmBG2-c2  29 tumorous blood cells mbn2  (42) ovary fGS/OSS  27 ovary OSC    17 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    56 Schneider line 2 Sg4    55 embryonic 1182-4H    32 embryonic GM2    41 embryonic Kc167    60 embryonic S1    23 embryonic S3    15 leg disc CME L1    40 wing disc CME-W2    34 wing disc ML-DmD8    29 wing disc ML-DmD9    30 wing disc ML-DmD16-c3    27 wing disc ML-DmD21    25 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    31 eye-antennal disc ML-DmD11    18 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    41 CNS ML-DmBG1-c1    21 CNS ML-DmBG2-c2    29 tumorous blood cells mbn2    42 ovary fGS/OSS    27 ovary OSC    17 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    56 Schneider line 2 Sg4    55 embryonic 1182-4H    32 embryonic GM2    41 embryonic Kc167    60 embryonic S1    23 embryonic S3    15 leg disc CME L1    40 wing disc CME-W2    34 wing disc ML-DmD8    29 wing disc ML-DmD9    30 wing disc ML-DmD16-c3    27 wing disc ML-DmD21    25 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    31 eye-antennal disc ML-DmD11    18 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    41 CNS ML-DmBG1-c1    21 CNS ML-DmBG2-c2    29 tumorous blood cells mbn2    42 ovary fGS/OSS    27 ovary OSC    17 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    25 cold shock, 4-day adult    22 heat shock, 4-day adult    31 Cadmium 50 mM 6 hrs, larvae L3    17 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    36 Cadmium 100 mM 48 hrs, 4-day adult    73 Copper 0.5 mM 12 hrs, larvae L3    21 Copper 15 mM 48 hrs, 4-day adult    52 Zinc 5 mM 12 hrs, larvae L3    14 Zinc 4.5 mM 48 hrs, 4-day adult    35 Ethanol 2.5% 3 hrs, larvae L3    58 Ethanol 5% 3 hrs, larvae L3    30 Ethanol 10% 3 hrs, larvae L3    16 Caffeine 1.5 mg/ml 4 hrs, larvae L3    21 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    59 Caffeine 25 mg/ml 48 hrs, 4-day adult    30 Paraquat 5 mM 48 hrs, 4-day adult    54 Paraquat 10 mM 48 hrs, 4-day adult    52 Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    25 cold shock, 4-day adult    22 heat shock, 4-day adult  (31) Cadmium 50 mM 6 hrs, larvae L3    17 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult  (36) Cadmium 100 mM 48 hrs, 4-day adult  (73) Copper 0.5 mM 12 hrs, larvae L3    21 Copper 15 mM 48 hrs, 4-day adult  (52) Zinc 5 mM 12 hrs, larvae L3    14 Zinc 4.5 mM 48 hrs, 4-day adult  (35) Ethanol 2.5% 3 hrs, larvae L3  (58) Ethanol 5% 3 hrs, larvae L3  (30) Ethanol 10% 3 hrs, larvae L3    16 Caffeine 1.5 mg/ml 4 hrs, larvae L3    21 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (59) Caffeine 25 mg/ml 48 hrs, 4-day adult  (30) Paraquat 5 mM 48 hrs, 4-day adult  (54) Paraquat 10 mM 48 hrs, 4-day adult  (52) Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    25 cold shock, 4-day adult    22 heat shock, 4-day adult    31 Cadmium 50 mM 6 hrs, larvae L3    17 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    36 Cadmium 100 mM 48 hrs, 4-day adult    73 Copper 0.5 mM 12 hrs, larvae L3    21 Copper 15 mM 48 hrs, 4-day adult    52 Zinc 5 mM 12 hrs, larvae L3    14 Zinc 4.5 mM 48 hrs, 4-day adult    35 Ethanol 2.5% 3 hrs, larvae L3    58 Ethanol 5% 3 hrs, larvae L3    30 Ethanol 10% 3 hrs, larvae L3    16 Caffeine 1.5 mg/ml 4 hrs, larvae L3    21 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    59 Caffeine 25 mg/ml 48 hrs, 4-day adult    30 Paraquat 5 mM 48 hrs, 4-day adult    54 Paraquat 10 mM 48 hrs, 4-day adult    52 Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    25 cold shock, 4-day adult    22 heat shock, 4-day adult    31 Cadmium 50 mM 6 hrs, larvae L3    17 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    36 Cadmium 100 mM 48 hrs, 4-day adult    73 Copper 0.5 mM 12 hrs, larvae L3    21 Copper 15 mM 48 hrs, 4-day adult    52 Zinc 5 mM 12 hrs, larvae L3    14 Zinc 4.5 mM 48 hrs, 4-day adult    35 Ethanol 2.5% 3 hrs, larvae L3    58 Ethanol 5% 3 hrs, larvae L3    30 Ethanol 10% 3 hrs, larvae L3    16 Caffeine 1.5 mg/ml 4 hrs, larvae L3    21 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    59 Caffeine 25 mg/ml 48 hrs, 4-day adult    30 Paraquat 5 mM 48 hrs, 4-day adult    54 Paraquat 10 mM 48 hrs, 4-day adult    52 Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    25 cold shock, 4-day adult    22 heat shock, 4-day adult    31 Cadmium 50 mM 6 hrs, larvae L3    17 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    36 Cadmium 100 mM 48 hrs, 4-day adult    73 Copper 0.5 mM 12 hrs, larvae L3    21 Copper 15 mM 48 hrs, 4-day adult    52 Zinc 5 mM 12 hrs, larvae L3    14 Zinc 4.5 mM 48 hrs, 4-day adult    35 Ethanol 2.5% 3 hrs, larvae L3    58 Ethanol 5% 3 hrs, larvae L3    30 Ethanol 10% 3 hrs, larvae L3    16 Caffeine 1.5 mg/ml 4 hrs, larvae L3    21 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    59 Caffeine 25 mg/ml 48 hrs, 4-day adult    30 Paraquat 5 mM 48 hrs, 4-day adult    54 Paraquat 10 mM 48 hrs, 4-day adult    52 Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    25 cold shock, 4-day adult    22 heat shock, 4-day adult  31 Cadmium 50 mM 6 hrs, larvae L3    17 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult  (36) Cadmium 100 mM 48 hrs, 4-day adult  (73) Copper 0.5 mM 12 hrs, larvae L3    21 Copper 15 mM 48 hrs, 4-day adult  (52) Zinc 5 mM 12 hrs, larvae L3    14 Zinc 4.5 mM 48 hrs, 4-day adult  (35) Ethanol 2.5% 3 hrs, larvae L3  (58) Ethanol 5% 3 hrs, larvae L3  30 Ethanol 10% 3 hrs, larvae L3    16 Caffeine 1.5 mg/ml 4 hrs, larvae L3    21 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (59) Caffeine 25 mg/ml 48 hrs, 4-day adult  30 Paraquat 5 mM 48 hrs, 4-day adult  (54) Paraquat 10 mM 48 hrs, 4-day adult  (52) Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    25 cold shock, 4-day adult    22 heat shock, 4-day adult    31 Cadmium 50 mM 6 hrs, larvae L3    17 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    36 Cadmium 100 mM 48 hrs, 4-day adult    73 Copper 0.5 mM 12 hrs, larvae L3    21 Copper 15 mM 48 hrs, 4-day adult    52 Zinc 5 mM 12 hrs, larvae L3    14 Zinc 4.5 mM 48 hrs, 4-day adult    35 Ethanol 2.5% 3 hrs, larvae L3    58 Ethanol 5% 3 hrs, larvae L3    30 Ethanol 10% 3 hrs, larvae L3    16 Caffeine 1.5 mg/ml 4 hrs, larvae L3    21 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    59 Caffeine 25 mg/ml 48 hrs, 4-day adult    30 Paraquat 5 mM 48 hrs, 4-day adult    54 Paraquat 10 mM 48 hrs, 4-day adult    52 Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    25 cold shock, 4-day adult    22 heat shock, 4-day adult    31 Cadmium 50 mM 6 hrs, larvae L3    17 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    36 Cadmium 100 mM 48 hrs, 4-day adult    73 Copper 0.5 mM 12 hrs, larvae L3    21 Copper 15 mM 48 hrs, 4-day adult    52 Zinc 5 mM 12 hrs, larvae L3    14 Zinc 4.5 mM 48 hrs, 4-day adult    35 Ethanol 2.5% 3 hrs, larvae L3    58 Ethanol 5% 3 hrs, larvae L3    30 Ethanol 10% 3 hrs, larvae L3    16 Caffeine 1.5 mg/ml 4 hrs, larvae L3    21 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    59 Caffeine 25 mg/ml 48 hrs, 4-day adult    30 Paraquat 5 mM 48 hrs, 4-day adult    54 Paraquat 10 mM 48 hrs, 4-day adult    52 Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_26 mE1_20_mRNA_expression_cluster_03
External Data & Images
Linkouts	BDGP expression data • SD04682 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0259174 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG32184-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL4005 Nedd4Scer\UAS.Long.T:Zzzz\FLAG lethal, with Scer\GAL4Act5C.PI Nedd4ΔNterm.Scer\UAS.Long.T:Zzzz\FLAG lethal, with Scer\GAL4da.G32 Nedd4Scer\UAS.Long.T:Zzzz\FLAG Nedd4ΔNterm.Scer\UAS.Long.T:Zzzz\FLAG lethal, with Scer\GAL4how-24B Nedd4Scer\UAS.Long.T:Zzzz\FLAG lethal, with Scer\GAL4αTub84B.PL Nedd4Scer\UAS.Long.T:Zzzz\FLAG Nedd4ΔNterm.Scer\UAS.Long.T:Zzzz\FLAG lethal | embryonic stage | maternal effect | recessive Nedd4unspecified lethal | embryonic stage | recessive Nedd4T119FS lethal | larval stage, with Scer\GAL4da.G32 Nedd4GS13178 lethal | third instar larval stage, with Scer\GAL4Act5C.PI Nedd4Scer\UAS.Long.T:Zzzz\FLAG partially lethal - majority die, with Scer\GAL4ptc-559.1 Nedd4GS13178 viable Nedd403 viable, with Scer\GAL4[-]: Nedd4GS13178 viable, with Scer\GAL4da.G32 Nedd4C974FS Nedd4D730FS Nedd4G693E Nedd4G746S Nedd4L613FS Nedd4L695F Nedd4L725FS Nedd4M884I Nedd4N121FS Nedd4N602FS Nedd4N728S Nedd4Q855stop Nedd4T119FS Nedd4V831D+del832-837 Nedd4W699stop Nedd4Y741H viable, with Scer\GAL4pnr-MD237 Nedd4GD5146 Sterility Allele fertile, with Scer\GAL4[-]: Nedd4GS13178 Other Phenotypes Allele locomotor behavior defective, with Scer\GAL4how-24B Nedd4Scer\UAS.Long.T:Zzzz\FLAG neuroanatomy defective | third instar larval stage, with Scer\GAL4005 Nedd4S39A.S645A.Scer\UAS.Long.T:Zzzz\FLAG Nedd4Scer\UAS.Long.T:Zzzz\FLAG Nedd4ΔMid.Scer\UAS.Long.T:Zzzz\FLAG Nedd4ΔNterm.Scer\UAS.Long.T:Zzzz\FLAG visible, with Scer\GAL4Dll-md23 Nedd4GS13178 visible, with Scer\GAL4dpp.blk1 Nedd4CA.Scer\UAS.T:Hsap\MYC visible, with Scer\GAL4ptc-559.1 Nedd4C974FS Nedd4GS13178 Nedd4Scer\UAS.T:Hsap\MYC Phenotype manifest in Allele anterior commissure Nedd4dsRNA.cMa anterior crossvein, with Scer\GAL4ptc-559.1 Nedd4C974FS embryonic central nervous system & axon Nedd4dsRNA.cMa intersegmental nerve branch ISNb of A1-7, with Scer\GAL4005 Nedd4Scer\UAS.Long.T:Zzzz\FLAG Nedd4S39A.S645A.Scer\UAS.Long.T:Zzzz\FLAG Nedd4ΔNterm.Scer\UAS.Long.T:Zzzz\FLAG Nedd4ΔMid.Scer\UAS.Long.T:Zzzz\FLAG longitudinal connective Nedd4dsRNA.cMa longitudinal connective & axon Nedd4dsRNA.cMa posterior commissure Nedd4dsRNA.cMa presumptive embryonic/larval central nervous system Nedd4dsRNA.cMa wing, with Scer\GAL4Dll-md23 Nedd4GS13178 wing, with Scer\GAL4ptc-559.1 Nedd4GS13178 Nedd4Scer\UAS.T:Hsap\MYC wing margin, with Scer\GAL4ptc-559.1 Nedd4GS13178 wing margin bristle, with Scer\GAL4Bx-MS1096 Nedd4GS13178 wing vein, with Scer\GAL4Bx-MS1096 Nedd4GS13178 wing vein, with Scer\GAL4Dll-md23 Nedd4GS13178 wing vein, with Scer\GAL4dpp.blk1 Nedd4CA.Scer\UAS.T:Hsap\MYC wing vein, with Scer\GAL4ptc-559.1 Nedd4GS13178 wing vein L3, with Scer\GAL4ptc-559.1 Nedd4GS13178
Classical Alleles ( 33 )
For All Classical Alleles Show
Allele of Nedd4 Class Mutagen Stocks Known lesion Nedd403 1 Yes Nedd4c00994 piggyBac transposase 1 -- Nedd4c01016 piggyBac transposase 1 -- Nedd4c01057 piggyBac transposase 1 -- Nedd4CB-0732-3 1 -- Nedd4d05280 P-element activity 1 -- Nedd4EY00500 P-element activity 1 -- Nedd4f00732 piggyBac transposase 1 -- Nedd4f03481 piggyBac transposase 1 -- Nedd4f04967 piggyBac transposase 1 -- Nedd4GS13178 1 -- Nedd4NP3029 1 -- Nedd4NP6623 1 -- Nedd4T119FS ethyl methanesulfonate 1 Yes Nedd4W699stop ethyl methanesulfonate 1 Yes Nedd4c01068 piggyBac transposase 0 -- Nedd4C974FS ethyl methanesulfonate 0 Yes Nedd4d05808 0 -- Nedd4D730FS ethyl methanesulfonate 0 Yes Nedd4DG05310 P-element activity 0 -- Nedd4G693E ethyl methanesulfonate 0 Yes Nedd4G746S ethyl methanesulfonate 0 Yes Nedd4L613FS ethyl methanesulfonate 0 Yes Nedd4L695F ethyl methanesulfonate 0 Yes Nedd4L725FS ethyl methanesulfonate 0 Yes Nedd4M884I ethyl methanesulfonate 0 Yes Nedd4N121FS ethyl methanesulfonate 0 Yes Nedd4N602FS ethyl methanesulfonate 0 Yes Nedd4N728S ethyl methanesulfonate 0 Yes Nedd4Q855stop ethyl methanesulfonate 0 Yes Nedd4unspecified 0 -- Nedd4V831D+del832-837 ethyl methanesulfonate 0 Yes Nedd4Y741H ethyl methanesulfonate 0 Yes
Alleles Carried on Transgenic Constructs ( 20 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Nedd4 Class Mutagen Stocks Known lesion Nedd4GD5146 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Nedd4HMS01221 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Nedd4JF01860 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Nedd4KK110286 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Nedd4C961A.Scer\UAS.Long.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes Nedd4C974FS.Scer\UAS.T:Ivir\HA1 in vitro construct - coding region fusion in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes Nedd4CA.Scer\UAS.T:Hsap\MYC in vitro construct - amino acid replacement in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Nedd4dsRNA.cMa in vitro construct - RNAi 0 Yes Nedd4dsRNA.cSa in vitro construct - RNAi 0 Yes Nedd4GD10810 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Nedd4NIG.7555R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Nedd4S39A.S645A.Scer\UAS.Long.T:Zzzz\FLAG 0 Yes Nedd4S444A.Scer\UAS.Short.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes Nedd4Scer\UAS.Long.T:Zzzz\FLAG 0 Yes Nedd4Scer\UAS.Short.T:Zzzz\FLAG in vitro construct - regulatory fusion 0 Yes Nedd4Scer\UAS.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Nedd4Scer\UAS.T:Ivir\HA1 in vitro construct - coding region fusion in vitro construct - regulatory fusion 0 Yes Nedd4Δhect.MtnA in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes Nedd4ΔMid.Scer\UAS.Long.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes Nedd4ΔNterm.Scer\UAS.Long.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3L)Exel9005 (Ryder, 2004.4.26) Df(3R)ED4688 (Keleman et al., 2005, Keleman et al., 2005)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD5146} NA P{KK110286} NA P{NIG.7555R} NA P{TRiP.HMS01221} NA P{TRiP.JF01860} NA P{UAS-Nedd4.CA} NA P{UAS-Nedd4.Lo.FLAG.C961A} NA P{UAS-Nedd4.Lo.FLAG.S39A.S645A} NA P{UAS-Nedd4.Lo.FLAG.ΔMid} NA P{UAS-Nedd4.Lo.FLAG.ΔNterm} NA P{UAS-Nedd4.Lo.FLAG} NA P{UAS-Nedd4.myc} NA P{UAS-Nedd4.S.FLAG.S444A} NA P{UAS-Nedd4.S.FLAG} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{PTT-GB}Edc3CB04145 NA P{RS3}Nedd4CB-0732-3 NA P{UAST-YFP.Rab26.T204N}Nedd403 NA P{wHy}Nedd4DG05310 NA PBac{PB}Nedd4c00994 NA PBac{PB}Nedd4c01016 NA PBac{PB}Nedd4c01057 NA PBac{PB}Nedd4c01068 NA PBac{WH}Nedd4f00732 NA PBac{WH}Nedd4f03481 NA PBac{WH}Nedd4f04967 NA insertion of enhancer trap binary system P{GawB}Nedd4NP3029 No P{GawB}Nedd4NP6623 No insertion of mobile activating element P{EPgy2}Nedd4EY00500 NA P{GSV1}C153C153 NA P{GSV6}Nedd4GS13178 NA P{XP}Nedd4d05280 NA P{XP}Nedd4d05808 NA
Gene Ontology: Function, Process & Cellular Component ( 17 unique terms )
Terms Based on Experimental Evidence ( 14 terms )
Molecular Function
CV term Evidence References
Notch binding inferred from physical interaction with N (assigned by UniProtKB) (Sakata et al., 2004) protein binding inferred from physical interaction with Myo10A (Liu et al., 2008) inferred from physical interaction with UniProtKB:Q24139 (assigned by UniProtKB) (Myat et al., 2002) protein domain specific binding inferred from direct assay (Kanelis et al., 2006) inferred from physical interaction with comm (Ing et al., 2007) inferred from physical interaction with N (Sakata et al., 2004)
Biological Process
CV term Evidence References
axon guidance inferred from mutant phenotype (Myat et al., 2002) NOT axon midline choice point recognition inferred from mutant phenotype (assigned by UniProtKB) (Keleman et al., 2005) axon midline choice point recognition inferred from mutant phenotype (assigned by UniProtKB) (Myat et al., 2002) negative regulation of Notch signaling pathway inferred from genetic interaction with UniProtKB:Q23985 (assigned by UniProtKB) (Sakata et al., 2004) inferred from mutant phenotype (Wilkin et al., 2004) inferred from mutant phenotype (assigned by UniProtKB) (Sakata et al., 2004) neuromuscular junction development inferred from mutant phenotype (Zhong et al., 2011) positive regulation of endocytosis inferred from mutant phenotype (Ing et al., 2007, Myat et al., 2002) positive regulation of receptor internalization inferred from mutant phenotype (Sakata et al., 2004) positive regulation of synapse assembly inferred from mutant phenotype (Ing et al., 2007) protein ubiquitination inferred from direct assay (assigned by UniProtKB) (Myat et al., 2002) inferred from mutant phenotype (Ing et al., 2007) inferred from mutant phenotype (assigned by UniProtKB) (Sakata et al., 2004) receptor internalization inferred from mutant phenotype (assigned by UniProtKB) (Sakata et al., 2004) inferred from physical interaction with UniProtKB:Q24139 (assigned by UniProtKB) (Myat et al., 2002)
Cellular Component
CV term Evidence References
cytoplasm inferred from direct assay (Ing et al., 2007, Zhong et al., 2011) inferred from direct assay (assigned by UniProtKB) (Sakata et al., 2004) plasma membrane inferred from direct assay (Zhong et al., 2011)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
ubiquitin-protein ligase activity inferred from biological aspect of ancestor with PANTHER:PTN000134256 (assigned by RefGenome) (Gaudet et al., 2010) inferred from sequence or structural similarity with mouse Nedd4 (FlyBase, 1992-)
Biological Process
CV term Evidence References
protein ubiquitination involved in ubiquitin-dependent protein catabolic process inferred from biological aspect of ancestor with PANTHER:PTN000134256 (assigned by RefGenome) (Gaudet et al., 2010)
Cellular Component
CV term Evidence References
cytoplasm inferred from biological aspect of ancestor with PANTHER:PTN000134256 (assigned by RefGenome) (Gaudet et al., 2010) nucleus inferred from biological aspect of ancestor with PANTHER:PTN000134256 (assigned by RefGenome) (Gaudet et al., 2010)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Nedd4 - 5-HT7 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nedd4 - CG14767 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nedd4 - CG15098 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nedd4 - CG32138 experimental knowledge based (Guruharsha et al., 2011) Nedd4 - CG8180 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Nedd4 - Flo-2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nedd4 - Kap-α3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nedd4 - Ndfip anti tag coimmunoprecipitation, peptide massfingerprinting, anti tag western blot (Mintseris et al., 2009.11.23, Dalton et al., 2011) Nedd4 - Nup50 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nedd4 - Su(dx) experimental knowledge based (Guruharsha et al., 2011) Nedd4 - Trap1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nedd4 - Tsp29Fb anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nedd4 - mib2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nedd4 - sty experimental knowledge based (Guruharsha et al., 2011)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Nedd4 allele Gene References Nedd4C974FS dx (Sakata, 2004) Nedd4CA.Scer\UAS.T:Hsap\MYC Su(dx) (Wilkin et al., 2004) Nedd4Scer\UAS.T:Hsap\MYC sli (Myat et al., 2002) Nedd4T119FS N (Sakata et al., 2004)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 65246 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0259174 DroID - A comprehensive database of gene and protein interactions. • FBgn0259174 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 39958 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q9VVI3
Orthologs
OrthoDB Orthologs (70) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG651F92 Dipteran inclusive ortholog search EOG6DBTNX Insect inclusive ortholog search EOG6GXDGP Arthropod inclusive ortholog search EOG61RN8V Metazoa inclusive ortholog search EOG689HD7
Orthologs in Drosophila Species (EOG651F92)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Nedd4     Drosophila simulans   Dsim\GD14716 Y   Drosophila sechellia   Dsec\GM25709 Y   Drosophila erecta   Dere\GG13624 Y   Drosophila yakuba   Dyak\GE19920 Y   Drosophila ananassae   Dana\GF10539 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA16742 Y   Drosophila persimilis   Dper\GL11879 Y   Drosophila willistoni   Dwil\GK17749 Y   Drosophila virilis   Dvir\GJ12273 Y   Drosophila mojavensis   Dmoj\GI12384 Y   Drosophila grimshawi   Dgri\GH16118 Y
Orthologs in non-Drosophila Dipterans (EOG6DBTNX)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL002536   Aedes aegypti Yellow fever mosquito  Aaeg\AAEL008374   Anopheles gambiae Malaria mosquito  Agam\AGAP000787   Anopheles gambiae Malaria mosquito  Agam\AGAP003133   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ011781   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ015113
Orthologs in non-Dipteran Insects (EOG6GXDGP)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB45517   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG018614   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH16564   Atta cephalotes Leafcutter ant  Acep\ACEP26420   Camponotus floridanus Florida carpenter ant  Cflo\CFLO18227   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL17265   Linepithema humile Argentine ant  Lhum\LH14757   Pogonomyrmex barbatus Red harvester ant  Pbar\PB16081   Solenopsis invicta Red fire ant  Sinv\SINV19584   Acyrthosiphon pisum Pea aphid  Apim\ACYPI001021   Bombyx mori Silkmoth  Bmor\BGIBMGA012636   Pediculus humanus Human body louse  Phum\PHUM236520   Tribolium castaneum Red flour beetle  Tcas\TC008896
Orthologs in non-Insect Arthropods (EOG61RN8V)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\Hn1l   Ixodes scapularis Deer tick  Isca\ISCW021604
Orthologs in non-Arthropod Metazoa (EOG689HD7)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\Y92H12A.2 Y Caenorhabditis elegans Nematode  Cele\wwp-1 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_007962gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_017871gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_024299gn Y Danio rerio Zebrafish  Drer\wwp2 Y Danio rerio Zebrafish  Drer\nedd4l Y Danio rerio Zebrafish  Drer\ENSDARG00000074903 Y Danio rerio Zebrafish  Drer\ENSDARG00000076149 Y Danio rerio Zebrafish  Drer\ENSDARG00000039778 Y Xenopus tropicalis Western clawed frog  Xtro\nedd4 Y Xenopus tropicalis Western clawed frog  Xtro\wwp2 Y Xenopus tropicalis Western clawed frog  Xtro\itch Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000029963 Y Xenopus tropicalis Western clawed frog  Xtro\nedd4l Y Gallus gallus Domestic chicken  Ggal\ENSGALG00000021339 Y Gallus gallus Domestic chicken  Ggal\ITCH Y Gallus gallus Domestic chicken  Ggal\NEDD4L Y Gallus gallus Domestic chicken  Ggal\WWP1 Y Gallus gallus Domestic chicken  Ggal\WWP2 Y Gallus gallus Domestic chicken  Ggal\NEDD4 Y Mus musculus House mouse  Mmus\Wwp1 Y Mus musculus House mouse  Mmus\Wwp2 Y Mus musculus House mouse  Mmus\Itch Y Mus musculus House mouse  Mmus\Nedd4 Y Mus musculus House mouse  Mmus\Nedd4l Y Rattus norvegicus Norway rat  Rnor\Nedd4l Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000024153 Y Rattus norvegicus Norway rat  Rnor\Nedd4 Y Rattus norvegicus Norway rat  Rnor\Wwp2 Y Rattus norvegicus Norway rat  Rnor\Wwp1 Y Rattus norvegicus Norway rat  Rnor\Itch Y Homo sapiens Human  Hsap\NEDD4L Y Homo sapiens Human  Hsap\NEDD4 Y Homo sapiens Human  Hsap\ITCH Y Homo sapiens Human  Hsap\WWP2 Y Homo sapiens Human  Hsap\WWP1 Y
Human Orthologs (5)
Gene OMIM HGNC Hsap\NEDD4L     Hsap\NEDD4     Hsap\ITCH     Hsap\WWP2     Hsap\WWP1
AAA Orthologs (12) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD14715 Drosophila simulans Dsim\GD14716 Drosophila sechellia Dsec\GM25709 Drosophila erecta Dere\GG13624 Drosophila yakuba Dyak\GE19920 Drosophila ananassae Dana\GF10539 Drosophila pseudoobscura pseudoobscura Dpse\GA16742 Drosophila persimilis Dper\GL11879 Drosophila willistoni Dwil\GK17749 Drosophila virilis Dvir\GJ12273 Drosophila mojavensis Dmoj\GI12384 Drosophila grimshawi Dgri\GH16118
Stocks & Reagents
Stocks Listed in FlyBase ( 18 )
Bloomington	31687 y1 v1; P{TRiP.JF01860}attP2
Harvard	- P{XP}Nedd4d05280 - PBac{PB}Nedd4c00994 - PBac{PB}Nedd4c01016 - PBac{PB}Nedd4c01057 - PBac{WH}Nedd4f00732 - PBac{WH}Nedd4f03481 - PBac{WH}Nedd4f04967
Kyoto	104347 w*; P{GawB}Nedd4NP3029 123301 w1118; P{RS3}Nedd4CB-0732-3
VDRC	v108475 P{KK110286}VIE-260B v13121 w1118; P{GD5146}v13121/TM3
	More stocks available...
Genomic Clones ( 1 )
	BACR44K07 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 132 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	IP05105 LD31220 SD04682
Other clones	DPiM_SD04682 MIP03063
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT02728 AT04181 HL03077 LD35563 LD39547 LD41989 LP08543 RE01885 RE02618 RE05183 RE05933 RE06774 RE07668 RE10207 RE15343 RE27124 RE28471 RE33904 RE37395 RE42087 RE48107 RE48685 RE49427 RE50107 RE51327 RE51485 RE55391 RE57581 RE66541 RE69966 SD14252
Other clones	8370 40093 45680 47491 54123 61729 61945 62121 63591 66870 81222 101399 153486 161037 161827 174195 176574 177190 214245.59 214283.60 214479.54 283389 523970 524669 533962 bs18h02 CK01.3C.E9 CK01.8D.B4 CK01.33A.B12 CK01.49C.F7 CK01.51D.G4 CK01.70D.F6 CK01.77D.H4 CK01.102A.E2 CK01.114A.D1 CK01.120C.F11 CK01.121D.F11 CK01.122A.G11 CK01.167A.G3 CK01.168D.D1 CK00535 Dmel_dig_003341_0647_0432 Dmel_dig_014237_0626_0339 Dmel_neb_015362_0592_0506 EK041124 EK056355 EK086049 EK092018 EK093128 EK102126 EK106311 EK137601 EK154044 EK162249 EK166314 EK175510 EK179314 EK186748 EK187432 EK189129 EK190318 EK194924 EK264815 EK266054 EK281149 EN04529 EN05715 EN06501 EN09927 EP05808 EP05838 EP16325 EP18411 FGC023H07 FGM002G11 FGM309E11 FGM324A09 IP04805 IP04905 IP05005 LP06620 RE01021 RP004025183 RP004095773 RP004107913 RP004195645 RP004197383 RP004200101 RP004201253 RP004224260 RP004233003 RP004234854 RP004386003 RP004397976 RP004487315 RP004556035
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0259174 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 39958
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Nedd4 CG7555 (Myat, 2000.7.21)
Source for database merge of	Source for merge of: Nedd4 CG32184 (FlyBase Genome Annotators, 2008)
Additional comments	Annotations CG7555 and CG32184 merged as CG42279 in release 5.8 of the genome annotation. (FlyBase Genome Annotators, 2008)
Other Comments
	dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) New annotation (CG32184) in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003) Identification: in a yeast two hybrid assay for proteins that interacts with the intracellular domain of comm. (Myat et al., 2002) comm is a specific binding partner and substrate of Nedd4. Nedd4 is recruited by comm protein to control cell surface levels of the robo receptor. (Myat et al., 2002)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_140736 NM_168736 NM_168737 NM_168738 NM_206394 NM_001144492 NM_001144493 NM_001275061 NM_001275062 RefSeq (Proteins) • NP_648993 NP_730282 NP_730283 NP_730284 NP_996116 NP_001137964 NP_001137965 NP_001261990 NP_001261991 DDBJ / EMBL / GenBank • AA140889 AA978552 AAF49328 AAL13848 AAL29143 AAN11694 AAN11695 AAN11696 AAS64973 ACL83319 ACL83320 AGB94683 AGB94684 AI533013 AI945962 AJ278468 AW944376 AY058619 AY061595 BZ748761 CAC42101 CZ466790 CZ466791 CZ466810 CZ466847 CZ466854 CZ474435 CZ483206 CZ486049 CZ486050 CZ487553 Entrez Gene - A searchable database of RefSeq genes. • 39958 UniProtKB/Swiss-Prot • Q9VVI3 UniProtKB/TrEMBL • B7Z067 B7Z068 Q8IQR5
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • C2 membrane targeting protein (IPR018029) C2 calcium-dependent membrane targeting (IPR000008) HECT (IPR000569) WW domain (IPR001202) C2 calcium/lipid-binding domain, CaLB (IPR008973) C2 domain (IPR020477) E3 ubiquitin-protein ligase, SMURF1 type (IPR024928) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • SD04682
Linkouts
	BioGRID - A database of protein and genetic interactions • 65246 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0259174 DroID - A comprehensive database of gene and protein interactions. • FBgn0259174 DRSC - Results from RNAi screens. • FBgn0259174 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0259174 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG32184-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0259174 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 39958 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 39958 modMine - Data generated by the modENCODE project. • FBgn0259174 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q9VVI3
Synonyms & Secondary IDs ( 8 )
Reported As
Symbol Synonym	CG7555 (Liu et al., 2008, Pile et al., 2003, Rotin, 2003.2.14, Mount, 2001.8.14, Myat, 2000.7.21, Molnar et al., 2006, Ing et al., 2007, Easow et al., 2007) CG32184 (Keleman et al., 2009.8.5) CG42279 (FlyBase Genome Annotators, 2008, Ni et al., 2008, Ni et al., 2010.12.1) DNedd4 (Wilkin et al., 2004, Akbar et al., 2005, Rosenzweig and Garrity, 2002, Couch and Condron, 2002, Myat et al., 2002, Tear et al., 2002, Wilkin et al., 2001, Myat, 2000.7.21, Akbar et al., 2007, Zhong et al., 2011) dNedd4 (Henry et al., 2000, Ing et al., 2007, Kanelis et al., 2006, Zhong et al., 2011, Dalton et al., 2011) dNedd4-1 (Henry et al., 2003) Nedd4 (Schoenherr et al., 2012, Yamada et al., 2008, Liu et al., 2008, Lu et al., 2006, Kawahashi et al., 2007, Buszczak et al., 2007, Zhang et al., 2007, Molnar et al., 2006, Hennig et al., 2006, Akdemir et al., 2007, Keleman et al., 2005, Wong et al., 2005, Sakata et al., 2004, Mudiganti et al., 2010, Tadros et al., 2007, Easow et al., 2007, Magalhaes et al., 2007, Jaekel and Klein, 2006, Japanese National Institute of Genetics, 2012.5.21, Munoz-Soriano et al., 2012) nedd4 (Sakata, 2004, Sakata et al., 2004, Munoz-Soriano et al., 2012)
Name Synonym	CG32184   CG42279 (FlyBase Genome Annotators, 2008) DNedd4 (Murphey and Godenschwege, 2002) Nedd4 (Murphey and Godenschwege, 2002, Easow et al., 2007)
Secondary FlyBase IDs
	FBgn0036736 FBgn0052184
References ( 78 )
Generate a list of	All references relating to this gene ( 78 )
List References by type	Research paper  ( 26 ) Supplementary material  ( 9 ) Review  ( 6 ) Personal communication to FlyBase  ( 15 ) Abstract  ( 11 ) FlyBase analysis  ( 4 ) Stock list  ( 1 ) Website  ( 2 ) Curated genome annotation  ( 2 ) Conference report  ( 2 )
Recent research papers ( 4 )
	Munoz-Soriano et al., 2012, J. Mol. Neurosci. 48(1): 136--143 Septin 4, the Drosophila Ortholog of Human CDCrel-1, Accumulates in parkin Mutant Brains and is Functionally Related to the Nedd4 E3 Ubiquitin Ligase. [FBrf0219099] Schoenherr et al., 2012, PLoS Genet. 8(5): e1002725 Drosophila activated cdc42 kinase has an anti-apoptotic function. [FBrf0218372] Dalton et al., 2011, Cell Death Differ. 18(7): 1150--1160 Drosophila Ndfip is a novel regulator of Notch signaling. [FBrf0214123] Zhong et al., 2011, PLoS ONE 6(11): e27007 A Splice Isoform of DNedd4, DNedd4-Long, Negatively Regulates Neuromuscular Synaptogenesis and Viability in Drosophila. [FBrf0216790] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...