General Information
Symbol
Dmel\bun
Species
D. melanogaster
Name
bunched
Annotation Symbol
CG42281
Feature Type
FlyBase ID
FBgn0259176
Gene Model Status
Stock Availability
Gene Snapshot
In progress.Contributions welcome.
Also Known As
shs, l(2)00255, Dm_2L:60722 , TSC-22
Genomic Location
Cytogenetic map
Sequence location
2L:12,455,540..12,546,611 [-]
Recombination map
2-46
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
GO Summary Ribbons
Families, Domains and Molecular Function
Gene Group Membership (FlyBase)
Protein Family (UniProt, Sequence Similarities)
Belongs to the TSC-22/Dip/Bun family. (Q24522Q24523)
Protein Domains/Motifs
Molecular Function (see GO section for details)
Experimental Evidence
-
Predictions / Assertions
Summaries
Gene Group Membership
OTHER DNA BINDING DOMAIN TRANSCRIPTION FACTORS -
The Other DNA binding domain transcription factors group is a collection of DNA-binding transcription factors that do not fit into any of the other major domain-based transcription factor groups.
UniProt Contributed Function Data
Probable transcription factor required for peripheral nervous system morphogenesis, eye development and oogenesis. May be required for the transmission of the dpp signal and for a morphogenetic movement of the medulla in the brain that reorients the second optic lobe relative to the first. Plays a role in determining proper dorsal cell fates leading to the formation of the dorsal appendages.
(UniProt, Q24522Q24523)
Gene Model and Products
Number of Transcripts
10
Number of Unique Polypeptides
9

Please see the GBrowse view of Dmel\bun or the JBrowse view of Dmel\bun for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Gene model reviewed during 5.49
Gene model reviewed during 5.43
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Gene model reviewed during 5.47
Gene model reviewed during 5.57
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0300521
5938
1206
FBtr0299656
2728
219
FBtr0299657
1754
189
FBtr0299652
3668
226
FBtr0300522
2832
254
FBtr0300523
7023
1097
FBtr0299658
5921
1331
FBtr0300524
2106
226
FBtr0339528
3490
192
FBtr0346622
5139
1137
Additional Transcript Data and Comments
Reported size (kB)
5.8, 2.8 (longest cDNA)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0289748
124.8
1206
6.27
FBpp0288931
22.7
219
4.21
FBpp0288932
19.7
189
4.42
FBpp0288927
23.5
226
4.35
FBpp0289749
26.4
254
4.63
FBpp0289750
113.4
1097
6.09
FBpp0288933
138.1
1331
6.24
FBpp0289751
23.5
226
4.35
FBpp0308611
19.6
192
4.35
FBpp0312202
118.5
1137
6.51
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

226 aa isoforms: bun-PD, bun-PH
Additional Polypeptide Data and Comments
Reported size (kDa)
1212, 224 (aa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\bun using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (21 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR000580
(assigned by InterPro )
inferred from sequence or structural similarity
Biological Process (17 terms)
Terms Based on Experimental Evidence (15 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR000580
(assigned by InterPro )
Cellular Component (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
inferred from direct assay
inferred from high throughput direct assay
Terms Based on Predictions or Assertions (0 terms)
Expression Data
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: maternally deposited

northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
The expression of this gene along with several others is induced in salivary glands in pupae at the time of head eversion. This stage is characterized by an increase in the ecdysone titer as well as large amounts of cell death in this tissue.
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
bun is expressed in mushroom body neurons during metamorphosis and is greatly reduced in adults. They are expressed in the alpha/beta neurons but not in alpha neurons or alpha'/beta' neurons.
bun protein is expressed in a stripe just anterior to the morphogenetic furrow.
Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
inferred from high throughput direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\bun in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, Transgenic Constructs and Phenotypes
Classical and Insertion Alleles ( 153 )
For All Classical and Insertion Alleles Show
 
Allele of bun
Class
Mutagen
Associated Insertion
Stocks
Known lesion
    0
    Yes
    Other relevant insertions
    miscellaneous insertions
    Name
    Expression Data
    Transgenic Constructs ( 17 )
    For All Alleles Carried on Transgenic Constructs Show
    Transgenic constructs containing/affecting coding region of bun
    Allele of bun
    Mutagen
    Associated Transgenic Construct
    Stocks
    Transgenic constructs containing regulatory region of bun
    Deletions and Duplications ( 11 )
    Summary of Phenotypes
    For more details about a specific phenotype click on the relevant allele symbol.
    Lethality
    Allele
    Sterility
    Allele
    Other Phenotypes
    Allele
    Phenotype manifest in
    Allele
    Orthologs
    Human Orthologs (via DIOPT v7.1)
    Homo sapiens (Human) (4)
    Species\Gene Symbol
    Score
    Best Score
    Best Reverse Score
    Alignment
    Complementation?
    Transgene?
    10 of 15
    Yes
    Yes
     
     
    5 of 15
    No
    Yes
     
     
    5 of 15
    No
    Yes
     
     
    4 of 15
    No
    Yes
     
    Model Organism Orthologs (via DIOPT v7.1)
    Mus musculus (laboratory mouse) (4)
    Species\Gene Symbol
    Score
    Best Score
    Best Reverse Score
    Alignment
    Complementation?
    Transgene?
    8 of 15
    Yes
    Yes
    6 of 15
    No
    Yes
    4 of 15
    No
    Yes
    3 of 15
    No
    Yes
    Rattus norvegicus (Norway rat) (5)
    5 of 13
    Yes
    Yes
    5 of 13
    Yes
    Yes
    4 of 13
    No
    Yes
    3 of 13
    No
    Yes
    2 of 13
    No
    Yes
    Xenopus tropicalis (Western clawed frog) (4)
    6 of 12
    Yes
    Yes
    3 of 12
    No
    Yes
    2 of 12
    No
    Yes
    1 of 12
    No
    Yes
    Danio rerio (Zebrafish) (5)
    7 of 15
    Yes
    Yes
    5 of 15
    No
    Yes
    5 of 15
    No
    Yes
    5 of 15
    No
    Yes
    1 of 15
    No
    Yes
    Caenorhabditis elegans (Nematode, roundworm) (3)
    4 of 15
    Yes
    Yes
    3 of 15
    No
    Yes
    1 of 15
    No
    Yes
    Arabidopsis thaliana (thale-cress) (0)
    No orthologs reported.
    Saccharomyces cerevisiae (Brewer's yeast) (0)
    No orthologs reported.
    Schizosaccharomyces pombe (Fission yeast) (0)
    No orthologs reported.
    Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091904BM )
    Organism
    Common Name
    Gene
    AAA Syntenic Ortholog
    Multiple Dmel Genes in this Orthologous Group
    Drosophila melanogaster
    fruit fly
    Drosophila suzukii
    Spotted wing Drosophila
    Drosophila simulans
    Drosophila sechellia
    Drosophila erecta
    Drosophila yakuba
    Drosophila ananassae
    Drosophila pseudoobscura pseudoobscura
    Drosophila persimilis
    Drosophila willistoni
    Drosophila virilis
    Drosophila mojavensis
    Drosophila grimshawi
    Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091504YA )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Musca domestica
    House fly
    Lucilia cuprina
    Australian sheep blowfly
    Mayetiola destructor
    Hessian fly
    Anopheles gambiae
    Malaria mosquito
    Culex quinquefasciatus
    Southern house mosquito
    Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W04TE )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Bombyx mori
    Silkmoth
    Danaus plexippus
    Monarch butterfly
    Heliconius melpomene
    Postman butterfly
    Apis florea
    Little honeybee
    Apis mellifera
    Western honey bee
    Bombus impatiens
    Common eastern bumble bee
    Bombus terrestris
    Buff-tailed bumblebee
    Linepithema humile
    Argentine ant
    Megachile rotundata
    Alfalfa leafcutting bee
    Nasonia vitripennis
    Parasitic wasp
    Tribolium castaneum
    Red flour beetle
    Pediculus humanus
    Human body louse
    Rhodnius prolixus
    Kissing bug
    Acyrthosiphon pisum
    Pea aphid
    Acyrthosiphon pisum
    Pea aphid
    Zootermopsis nevadensis
    Nevada dampwood termite
    Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X04PP )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Strigamia maritima
    European centipede
    Ixodes scapularis
    Black-legged tick
    Stegodyphus mimosarum
    African social velvet spider
    Stegodyphus mimosarum
    African social velvet spider
    Daphnia pulex
    Water flea
    Daphnia pulex
    Water flea
    Daphnia pulex
    Water flea
    Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G06PM )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Strongylocentrotus purpuratus
    Purple sea urchin
    Strongylocentrotus purpuratus
    Purple sea urchin
    Ciona intestinalis
    Vase tunicate
    Gallus gallus
    Domestic chicken
    Gallus gallus
    Domestic chicken
    Gallus gallus
    Domestic chicken
    Human Disease Model Data
    FlyBase Human Disease Model Reports
      Alleles Reported to Model Human Disease (Disease Ontology)
      Download
      Models ( 0 )
      Allele
      Disease
      Evidence
      References
      Interactions ( 0 )
      Allele
      Disease
      Interaction
      References
      Comments ( 0 )
       
      Human Orthologs (via DIOPT v7.1)
      Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
      Homo sapiens (Human)
      Gene name
      Score
      OMIM
      OMIM Phenotype
      Complementation?
      Transgene?
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Dmel gene
      Ortholog showing functional complementation
      Supporting References
      Interactions
      Summary of Physical Interactions
      esyN Network Diagram
      Show neighbor-neighbor interactions:
      Select Layout:
      Legend:
      Protein
      RNA
      Selected Interactor(s)
      Interactions Browser

      Please look at the Interaction Group reports for full details of the physical interactions
      protein-protein
      Interacting group
      Assay
      References
      Summary of Genetic Interactions
      esyN Network Diagram
      esyN Network Key:
      Suppression
      Enhancement

      Please look at the allele data for full details of the genetic interactions
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      suppressible
      suppressible
      External Data
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      Pathways
      Gene Group - Pathway Membership (FlyBase)
      External Data
      Linkouts
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      2L
      Recombination map
      2-46
      Cytogenetic map
      Sequence location
      2L:12,455,540..12,546,611 [-]
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      33E7-33E9
      Limits computationally determined from genome sequence between P{EP}ElfEP476&P{EP}bunEP488 and P{lacW}bunk00612&P{EP}EP682EP682
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      33E7-33E8
      (determined by in situ hybridisation)
      33E-33E
      (determined by in situ hybridisation)
      33F-33F
      (determined by in situ hybridisation)
      33E9-33E10
      33F1--2 38B4--5
      Experimentally Determined Recombination Data
      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (77)
      Genomic Clones (55)
      cDNA Clones (217)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequences
      BDGP DGC clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      Other clones
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      Antibody Information
      Laboratory Generated Antibodies
       
      polyclonal
      Commercially Available Antibodies
       
      Other Information
      Relationship to Other Genes
      Source for database identify of
      Source for identity of: bun CG5461
      Source for database merge of
      Source for merge of: bun CG31857
      Additional comments
      Annotations CG5461 and CG31857 merged as CG42281 in release 5.8 of the genome annotation.
      Other Comments
      bun regulates the level of N stimulation to position the boundary between centripetally migrating and stationary columnar cells.
      New annotation (CG31857) in release 3 of the genome annotation.
      bun integrates opposing dpp and EGF signals to set the operculum boundary in the developing egg.
      Identification: Enhancer trap expression pattern survey for loci expressed in the ring gland.
      bun has been cloned and sequenced.
      The autosomal "FLP-DFS" technique (using the P{ovoD1-18} P{FRT(whs)} P{hsFLP} chromosomes) has been used to identify the specific maternal effect phenotype for the zygotic lethal mutation.
      Mutations in bun cause a delay in photoreceptor neural differentiation relative to dpp expression suggesting that bun may be required for the transmission of the dpp signal. bun has a role in inducing rotation of the second optic lobe. bun encodes a cytoplasmic protein expressed anterior to the furrow in a hh-dependent manner.
      Origin and Etymology
      Discoverer
      Etymology
      Identification
      External Crossreferences and Linkouts ( 108 )
      Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
      Other crossreferences
      BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
      InterPro - A database of protein families, domains and functional sites
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center cDNA clones
      DPiM - Drosophila Protein interaction map
      DroID - A comprehensive database of gene and protein interactions.
      DRSC - Results frm RNAi screens
      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
      FLIGHT - Cell culture data for RNAi and other high-throughput technologies
      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Flygut - An atlas of the Drosophila adult midgut
      FlyMine - An integrated database for Drosophila genomics
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      KEGG Genes - Molecular building blocks of life in the genomic space.
      modMine - A data warehouse for the modENCODE project
      Synonyms and Secondary IDs (24)
      Reported As
      Symbol Synonym
      CG31857
      Tsc22
      l(2)k02903
      l(2)rI043
      Secondary FlyBase IDs
      • FBgn0010460
      • FBgn0010670
      • FBgn0011724
      • FBgn0014385
      • FBgn0051857
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      References (185)