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Gene Dmel\bun

General Information
Symbol	Dmel\bun	Species	D. melanogaster
Name	bunched	Annotation symbol	CG42281
Feature type	protein_coding_gene	FlyBase ID	FBgn0259176
Gene Model Status	Current	Stock availability	67 publicly available
Also Known As	shs, CG5461, l(2)00255, TSC-22
Genomic Location
Chromosome (arm)	2L	Recombination map	2-
Cytogenetic map	33E5-33E9	Sequence location	2L:12,455,540..12,546,630 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene bunched is referred to in FlyBase by the symbol Dmel\bun (CG42281, FBgn0259176). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: protein homodimerization activity. There is experimental evidence for 13 unique biological process terms, many of which group under: biological regulation; ovarian follicle cell development; programmed cell death; multicellular organism reproduction; positive regulation of biological process; gamete generation; positive regulation of cell proliferation; cellular component organization or biogenesis; localization; regionalization; gland morphogenesis; sensory organ development. 145 alleles are reported. The phenotypes of these alleles are annotated with: organ system subdivision; organ system; external compound sense organ; adult segment; peripheral nervous system; acellular anatomical structure; thoracic segment; histaminergic neuron; epithelial cell; eo support cell. It has 9 annotated transcripts and 9 annotated polypeptides. Protein features are: TSC-22 / Dip / Bun. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 00-06 and 12-24 hour embryonic stages, during late larval stages, at stages throughout the pupal period, in stages of adults of both sexes. Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at high levels in the following post-embryonic organs or tissues: adult eye, adult central nervous system, adult salivary gland, larval trachea. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval salivary gland, adult female reproductive system, adult male accessory gland, larval/adult carcass. Gene sequence location is 2L:12455540..12546630.
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	References Knowles-Barley, 2011.8.24, BrainTrap expression curation.
FB2012_01	Polypeptides bun-PI Transcripts bun-RI References Spradling et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(38): 15948--15953 Carreira et al., 2011, BMC Dev. Biol. 11: 32 Sequence features HFA01255 HFA03500 BKN24616 HFA01253 BKN42242 Clones ESG019c.E21_C03 ESG0111c.B21_H06 EK040914 108716 CK01.89A.E4 145649 119404 FGM321D03 283580 ESG0117a.B21_E04 RH08983 EK239150 146168 EK308828 EK193119 44781 EK164409 EK248563 EN02725 EK248358 56972 EC258011 CK01.48D.D3 145748 EN09620 EK067909 EK249142 EK160241 EK171604 CK01.106A.G5 174834 65776 296185 EK088527 EK070050
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 33E5-33E9   Limits computationally determined from genome sequence between P{EP}ElfEP476&P{EP}bunEP488 and P{lacW}bunk00612&P{EP}EP682EP682
Experimentally Determined Cytological Location
Cytogenetic map Notes References 33E7-33E8     (Treisman, 1995.9.29) 33E7-33E8   (determined by in situ hybridisation)   (Spradling et al., 1999) 33E-33E   (determined by in situ hybridisation)   (Dobens et al., 1997) 33E7-33E8   (determined by in situ hybridisation)   (Perrimon et al., 1996) 33F-33F   (determined by in situ hybridisation)   (Kania et al., 1995) 33E9-33E10   33F1--2 38B4--5   (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location	2- (Treisman, 1995.9.29)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\bun for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.43 Gene merge based on EST/cDNA data Due to an oversight in the implementation of gene annotation merges, the symbols for some of the transcripts and polypeptides of this gene were changed to non-unique designations in r5.8. Although there were no conflicts within a given release, the same symbols were used for different isoforms in different releases. The affected gene product symbols were changed to symbols not used previously in the database in r5.13. Based on a community request and usage in published work, they were changed back to the original symbols in r5.16.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) bun-RA FBtr0300521  NM_080364   5406   1206 bun-RB FBtr0299656  NM_001169494   2713   219 bun-RC FBtr0299657  NM_165013   1735   189 bun-RD FBtr0299652  NM_001169493   3668   226 bun-RE FBtr0300522  NM_001042893   2832   254 bun-RF FBtr0300523  NM_001042894   7042   1097 bun-RG FBtr0299658  NM_001042892   5940   1331 bun-RH FBtr0300524  NM_165012   2106   226 bun-RI FBtr0329826     3486   192
Additional Transcript Data & Comments
Reported size (kB)	5.8, 2.8 (longest cDNA) (Treisman et al., 1995)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein bun-PA   FBpp0289748   124.8   1206   6.27     NP_525103   AAF53201 bun-PB   FBpp0288931   22.7   219   4.21     NP_001162965   ACZ94251 bun-PC   FBpp0288932   19.7   189   4.42     NP_723756   AAN10817 bun-PD   FBpp0288927   23.5   226   4.35     NP_001162964   ACZ94250 bun-PE   FBpp0289749   26.4   254   4.63     NP_001036358   ABI31312 bun-PF   FBpp0289750   113.4   1097   6.09     NP_001036359   ABI31313 bun-PG   FBpp0288933   138.1   1331   6.24     NP_001036357   ABI31311 bun-PH   FBpp0289751   23.5   226   4.35     NP_723755   AAF53200 bun-PI   FBpp0302875   19.6   192   4.35
Additional Polypeptide Data & Comments
Reported size (kDa)	1212, 224 (aa) (Treisman et al., 1995)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • TSC-22 / Dip / Bun (IPR000580)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA697512   AA697769   AA698826   AE014134 AAF53200   AE014134 AAF53201   AE014134 AAN10817   AE014134 ABI31311   AE014134 ABI31312   AE014134 ABI31313   AE014134 ACZ94250   AE014134 ACZ94251   AF003342 AAB61239   AF171800   AI107641   AI135873   AI258317   AI513268   AI946452   AQ024970   AQ026018   AQ034170   AQ073593   AQ073735   AY060295 AAL25334   BG635430   BG639400   BH011405   BH759209   BH759229   BH840697   BH854538   BI162478   BI162479   BI162927   BI168840   BI172050   BI229693   BI235152   BI235176   BI235825   BI237921   BI238928   BI242754   BI358200   BI362824   BI364050   BI368803   BI369005   BI369522   BI373470   BI573306   BI573307   BI626670   BI627011   BP540778   BP542131   BP542325   BP543523   BP543524   BP544924   BP544925   BP548166   BP548854   BP549353   BP557295   BP559512   BP559513   BT025127   BT122061   CA804014   CA804248   CA804789   CA804936   CA805099   CA805221   CA806096   CA807775   CL844389   CO153746   CO156940   CO188554   CO190302   CO263439   CO269604   CO269732   CO270211   CO271231   CO272935   CO276024   CO277549   CO280532   CO282335   CO283770   CO288168   CO289083   CO289299   CO293971   CO295556   CO296003   CO297069   CO297704   CO298146   CO298244   CO301731   CO304860   CO305368   CO305430   CO305640   CO306075   CO307481   CO307573   CO311134   CO312471   CO314004   CO314999   CO315116   CO317244   CO321342   CO321763   CO324277   CO325670   CO329853   CO330637   CO330926   CO334078   CO335343   CO337306   CO341113   CO341824   CO342659   CZ468646   CZ469597   CZ469883   CZ469884   CZ471066   CZ471108   CZ471907   CZ474523   CZ474750   CZ475062   CZ475201   CZ475762   CZ475763   CZ476281   CZ477188   CZ477189   CZ477276   CZ480758   CZ480770   CZ482440   CZ482441   CZ482640   CZ484634   CZ486537   CZ489808   EC053674   EC056648   EC059864   EC069600   EC075351   EC076926   EC080848   EC081619   EC082270   EC082747   EC083672   EC086420   EC087679   EC088794   EC195263   EC197521   EC197955   EC200367   EC201852   EC202180   EC204130   EC211479   EC211730   EC214705   EC216359   EC219674   EC219838   EC222468   EC223769   EC225788   EC227398   EC227820   EC232287   EC237503   EC242748   EC248633   EC249069   EC249274   EC249462   EC251351   EC252738   EC258011   EC263132   EC264038   EC266103   EC267317   EC267966   EL878318   EL878319   EL878441   EL882055   ES691463   ES693599   EV589299   EV591131   G00410   GH748776   GH751507   GH757927   GH761339   GH778029   GH780878   GH783152   GH786510   GH787389   GH805058   GH806688   GH813530   GH819814   GH819836   GH828187   GH829404   GH835198   GH841898   GH842246   GH846543   GH847665   GH849774   GH849977   GH850258   GH851343   GH856136   GH865173   GH870337   GH870486   GH879737   GH880120   GH887669   GH889706   GH895249   GH902590   GH906350   GH908788   GH909489   GH910143   GH912031   GH913083   GH914045   GH920006   GH921635   GH921776   GH926005   GH942427   L42511 AAC41607   L42512 AAC41608
UniProtKB/Swiss-Prot	Q24522 Q24523
UniProtKB/TrEMBL	E1JHG7 E1JH96
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
northern blot Stage Tissue/Position (including subcellular localization) Reference pupal stage   salivary gland   (Lee et al., 2003)
Additional Descriptive Data
	The expression of this gene along with several others is induced in salivary glands in pupae at the time of head eversion. This stage is characterized by an increase in the ecdysone titer as well as large amounts of cell death in this tissue. (Lee et al., 2003)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference third instar larval stage   eye-antennal disc   (Treisman et al., 1995)
Additional Descriptive Data
	bun protein is expressed in a stripe just anterior to the morphogenetic furrow. (Treisman et al., 1995)
Marker for
Subcellular Localization
CV Term	cytoplasm (Treisman et al., 1995)
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0259176    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0259176 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 00-06 and 12-24 hour embryonic stages, during late larval stages, at stages throughout the pupal period, in stages of adults of both sexes. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0259176 expression level Developmental Stage   Expression Level embryo 00-02hr    2950 embryo 02-04hr    1449 embryo 04-06hr    1104 embryo 06-08hr    1037 embryo 08-10hr    906 embryo 10-12hr    1036 embryo 12-14hr    1216 embryo 14-16hr    1293 embryo 16-18hr    1590 embryo 18-20hr    2508 embryo 20-22hr    1825 embryo 22-24hr    2057 larva L1    1113 larva L2    706 larva L3 12hr old    776 larva L3 puffstage 1-2    599 larva L3 puffstage 3-6    1402 larva L3 puffstage 7-9    1704 white prepupae new    1896 white prepupae 12hr    3554 white prepupae 24hr    1916 pupae 2d postWPP    1673 pupae 3d postWPP    1187 pupae 4d postWPP    1309 adult male 01day    1405 adult male 05day    1901 adult male 30day    1869 adult female 01day    1461 adult female 05day    1875 adult female 30day    1983 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (2950) embryo 02-04hr  (1449) embryo 04-06hr  (1104) embryo 06-08hr  (1037) embryo 08-10hr  (906) embryo 10-12hr  (1036) embryo 12-14hr  (1216) embryo 14-16hr  (1293) embryo 16-18hr  (1590) embryo 18-20hr  (2508) embryo 20-22hr  (1825) embryo 22-24hr  (2057) larva L1  (1113) larva L2  (706) larva L3 12hr old  (776) larva L3 puffstage 1-2  (599) larva L3 puffstage 3-6  (1402) larva L3 puffstage 7-9  (1704) white prepupae new  (1896) white prepupae 12hr  (3554) white prepupae 24hr  (1916) pupae 2d postWPP  (1673) pupae 3d postWPP  (1187) pupae 4d postWPP  (1309) adult male 01day  (1405) adult male 05day  (1901) adult male 30day  (1869) adult female 01day  (1461) adult female 05day  (1875) adult female 30day  (1983) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (2950) embryo 02-04hr  1449 embryo 04-06hr    1104 embryo 06-08hr    1037 embryo 08-10hr    906 embryo 10-12hr    1036 embryo 12-14hr    1216 embryo 14-16hr    1293 embryo 16-18hr  (1590) embryo 18-20hr  (2508) embryo 20-22hr  (1825) embryo 22-24hr  (2057) larva L1    1113 larva L2    706 larva L3 12hr old    776 larva L3 puffstage 1-2    599 larva L3 puffstage 3-6  1402 larva L3 puffstage 7-9  (1704) white prepupae new  (1896) white prepupae 12hr  (3554) white prepupae 24hr  (1916) pupae 2d postWPP  (1673) pupae 3d postWPP    1187 pupae 4d postWPP    1309 adult male 01day  1405 adult male 05day  (1901) adult male 30day  (1869) adult female 01day  1461 adult female 05day  (1875) adult female 30day  (1983) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    2950 embryo 02-04hr    1449 embryo 04-06hr    1104 embryo 06-08hr    1037 embryo 08-10hr    906 embryo 10-12hr    1036 embryo 12-14hr    1216 embryo 14-16hr    1293 embryo 16-18hr    1590 embryo 18-20hr    2508 embryo 20-22hr    1825 embryo 22-24hr    2057 larva L1    1113 larva L2    706 larva L3 12hr old    776 larva L3 puffstage 1-2    599 larva L3 puffstage 3-6    1402 larva L3 puffstage 7-9    1704 white prepupae new    1896 white prepupae 12hr    3554 white prepupae 24hr    1916 pupae 2d postWPP    1673 pupae 3d postWPP    1187 pupae 4d postWPP    1309 adult male 01day    1405 adult male 05day    1901 adult male 30day    1869 adult female 01day    1461 adult female 05day    1875 adult female 30day    1983 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    2950 embryo 02-04hr    1449 embryo 04-06hr    1104 embryo 06-08hr    1037 embryo 08-10hr    906 embryo 10-12hr    1036 embryo 12-14hr    1216 embryo 14-16hr    1293 embryo 16-18hr    1590 embryo 18-20hr    2508 embryo 20-22hr    1825 embryo 22-24hr    2057 larva L1    1113 larva L2    706 larva L3 12hr old    776 larva L3 puffstage 1-2    599 larva L3 puffstage 3-6    1402 larva L3 puffstage 7-9    1704 white prepupae new    1896 white prepupae 12hr    3554 white prepupae 24hr    1916 pupae 2d postWPP    1673 pupae 3d postWPP    1187 pupae 4d postWPP    1309 adult male 01day    1405 adult male 05day    1901 adult male 30day    1869 adult female 01day    1461 adult female 05day    1875 adult female 30day    1983 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0259176 expression level Developmental Stage   Expression Level embryo 00-02hr    2950 embryo 02-04hr    1449 embryo 04-06hr    1104 embryo 06-08hr    1037 embryo 08-10hr    906 embryo 10-12hr    1036 embryo 12-14hr    1216 embryo 14-16hr    1293 embryo 16-18hr    1590 embryo 18-20hr    2508 embryo 20-22hr    1825 embryo 22-24hr    2057 larva L1    1113 larva L2    706 larva L3 12hr old    776 larva L3 puffstage 1-2    599 larva L3 puffstage 3-6    1402 larva L3 puffstage 7-9    1704 white prepupae new    1896 white prepupae 12hr    3554 white prepupae 24hr    1916 pupae 2d postWPP    1673 pupae 3d postWPP    1187 pupae 4d postWPP    1309 adult male 01day    1405 adult male 05day    1901 adult male 30day    1869 adult female 01day    1461 adult female 05day    1875 adult female 30day    1983 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (2950) embryo 02-04hr  (1449) embryo 04-06hr  (1104) embryo 06-08hr  (1037) embryo 08-10hr  (906) embryo 10-12hr  (1036) embryo 12-14hr  (1216) embryo 14-16hr  (1293) embryo 16-18hr  (1590) embryo 18-20hr  (2508) embryo 20-22hr  (1825) embryo 22-24hr  (2057) larva L1  (1113) larva L2  (706) larva L3 12hr old  (776) larva L3 puffstage 1-2  (599) larva L3 puffstage 3-6  (1402) larva L3 puffstage 7-9  (1704) white prepupae new  (1896) white prepupae 12hr  (3554) white prepupae 24hr  (1916) pupae 2d postWPP  (1673) pupae 3d postWPP  (1187) pupae 4d postWPP  (1309) adult male 01day  (1405) adult male 05day  (1901) adult male 30day  (1869) adult female 01day  (1461) adult female 05day  (1875) adult female 30day  (1983) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (2950) embryo 02-04hr  1449 embryo 04-06hr    1104 embryo 06-08hr    1037 embryo 08-10hr    906 embryo 10-12hr    1036 embryo 12-14hr    1216 embryo 14-16hr    1293 embryo 16-18hr  1590 embryo 18-20hr  (2508) embryo 20-22hr  1825 embryo 22-24hr  2057 larva L1    1113 larva L2    706 larva L3 12hr old    776 larva L3 puffstage 1-2    599 larva L3 puffstage 3-6  1402 larva L3 puffstage 7-9  1704 white prepupae new  1896 white prepupae 12hr  (3554) white prepupae 24hr  1916 pupae 2d postWPP  1673 pupae 3d postWPP    1187 pupae 4d postWPP    1309 adult male 01day  1405 adult male 05day  1901 adult male 30day  1869 adult female 01day  1461 adult female 05day  1875 adult female 30day  1983 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    2950 embryo 02-04hr    1449 embryo 04-06hr    1104 embryo 06-08hr    1037 embryo 08-10hr    906 embryo 10-12hr    1036 embryo 12-14hr    1216 embryo 14-16hr    1293 embryo 16-18hr    1590 embryo 18-20hr    2508 embryo 20-22hr    1825 embryo 22-24hr    2057 larva L1    1113 larva L2    706 larva L3 12hr old    776 larva L3 puffstage 1-2    599 larva L3 puffstage 3-6    1402 larva L3 puffstage 7-9    1704 white prepupae new    1896 white prepupae 12hr    3554 white prepupae 24hr    1916 pupae 2d postWPP    1673 pupae 3d postWPP    1187 pupae 4d postWPP    1309 adult male 01day    1405 adult male 05day    1901 adult male 30day    1869 adult female 01day    1461 adult female 05day    1875 adult female 30day    1983 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    2950 embryo 02-04hr    1449 embryo 04-06hr    1104 embryo 06-08hr    1037 embryo 08-10hr    906 embryo 10-12hr    1036 embryo 12-14hr    1216 embryo 14-16hr    1293 embryo 16-18hr    1590 embryo 18-20hr    2508 embryo 20-22hr    1825 embryo 22-24hr    2057 larva L1    1113 larva L2    706 larva L3 12hr old    776 larva L3 puffstage 1-2    599 larva L3 puffstage 3-6    1402 larva L3 puffstage 7-9    1704 white prepupae new    1896 white prepupae 12hr    3554 white prepupae 24hr    1916 pupae 2d postWPP    1673 pupae 3d postWPP    1187 pupae 4d postWPP    1309 adult male 01day    1405 adult male 05day    1901 adult male 30day    1869 adult female 01day    1461 adult female 05day    1875 adult female 30day    1983 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0259176    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0259176 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at high levels in the following post-embryonic organs or tissues: adult eye, adult central nervous system, adult salivary gland, larval trachea. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval salivary gland, adult female reproductive system, adult male accessory gland, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Heatmap Tissue   Expression Level Larval Central Nervous System no informative data Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea no informative data Larval Carcass no informative data Adult Head no informative data Adult Eye no informative data Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary no informative data Adult Testis no informative data Adult Male Accessory Gland no informative data Adult Carcass no informative data modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_27
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0259176 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG31857-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL4Act5C.PU bunA.Scer\UAS bunGE12327 lethal (with bun04230) bunout4 bunout12 bunout14 bunrI043 lethal (with bun06903) bunout4 bunout12 bunout14 bunrI043 lethal (with bunk02903) bunout4 bunout12 bunout14 lethal (with bunout4) bun04230 bun06903 bunk02903 lethal (with bunout12) bun04230 bun06903 bunk02903 lethal (with bunout14) bun04230 bun06903 bunk02903 lethal (with bunrI043) bun04230 bun06903 lethal (with Df(2L)prd1.7) bunout4 bunout12 bunout14 lethal | embryonic stage | partially | rescuable maternal effect bun00255 lethal | larval stage | recessive bun00255 bunA-149B bunA-211B lethal | maternal effect | recessive bunrI043 lethal | pupal stage | recessive bunk09036 lethal | recessive bun00255 bun585RSX bun01550 bun02687 bun03504 bun04230 bun05479 bun06708 bun06903 bun07209 bun07692 bunA-P519L bunA-Q578X bunA-Q609X bunA-Q879X bunA-Q922X bunA-Q988X bunA-R508W bunk02603 bunk02903a bunk06713 bunk09018 bunk10318 bunrH541 bunrI043 bunrI482 lethal | recessive | third instar larval stage bun200B lethal | second instar larval stage (with Df(2L)Exel6033) bunA-149B bunA-211B lethal | third instar larval stage (with Df(2L)Exel6033) bunA-149B bunA-211B semi-lethal | pupal stage, with Scer\GAL4pnr-MD237 bunGD1392 semi-viable (with bunout4) bunrI043 semi-viable (with bunout12) bunrI043 semi-viable (with bunout14) bunrI043 semi-viable (with bunrI043) bunout4 bunout12 bunout14 viable bunB-132A bunB-181A bunC-158B bunCB03431 bunEY11024 bunGE12327 viable, with Scer\GAL4pnr-MD237 bunGD10387 Sterility Allele female sterile | recessive bunrI043 fertile bunB-132A bunB-181A bunC-158B bunEY11024 Other Phenotypes Allele cell shape defective | somatic clone bununspecified decreased cell number bunGE12327 decreased cell number (with bunA-149B) bunGE12327 decreased cell number (with bunGE12327) bunA-149B decreased cell number | dominant bunA-149B decreased cell number | recessive | somatic clone bunA-Q578X decreased cell number | somatic clone bun200B bunA-149B bunA-Q922X decreased cell size bunGE12327 decreased cell size (with bunA-149B) bunGE12327 decreased cell size (with bunGE12327) bunA-149B decreased cell size | dominant bunA-149B decreased cell size | recessive | somatic clone bunA-Q578X decreased cell size | somatic clone bunA-Q922X developmental rate defective bunGE12327 developmental rate defective | larval stage (with Df(2L)Exel6033) bun200B bunA-149B bunA-211B small body (with bunout4) bunrI043 small body (with bunout12) bunrI043 small body (with bunout14) bunrI043 small body (with bunrI043) bunout4 bunout12 bunout14 small body | adult stage | dominant | female bunA-149B small body | adult stage | dominant | male bunA-149B small body | adult stage | female | recessive bunGE12327 small body | female (with bunA-149B) bunGE12327 small body | female (with bunGE12327) bunA-149B small body | larval stage (with Df(2L)Exel6033) bun200B small body | male (with bunA-149B) bunGE12327 small body | male (with bunGE12327) bunA-149B visible bunrI043 visible, with Scer\GAL4pnr-MD237 bunGD1392 visible | recessive | somatic clone bun200B bun585RSX bunA-P519L bunA-Q578X bunA-Q609X bunA-Q879X bunA-Q922X bunA-Q988X bunA-R508W wild-type buncDNA2.GMR Phenotype manifest in Allele adult head | somatic clone bunA-R508W bunA-P519L bunA-Q578X bun585RSX bunA-Q609X bunA-Q879X bunA-Q988X bunA-Q922X bunA-149B bun200B centripetally migrating follicle cell, with Scer\GAL4Act5C.PP bun1.Scer\UAS centripetally migrating follicle cell | somatic clone bununspecified chordotonal organ | lateral bunk09036 dorsal appendage bunrI043 dorsal appendage | maternal effect | somatic clone bun04230 bun06903 bunrI043 egg | maternal effect | somatic clone bun04230 bun06903 bunrI043 egg chamber | somatic clone bununspecified egg operculum bunrI043 embryonic/first instar larval cuticle bun00255 embryonic segment bun00255 follicle cell, with Scer\GAL4Act5C.PP bunX.Scer\UAS germline cell | male, with Scer\GAL4ptc-559.1 bunEP2622 leg (with bunout4) bunrI043 leg (with bunout12) bunrI043 leg (with bunout14) bunrI043 leg (with bunrI043) bunout12 bunout14 bunout4 mesothoracic tergum, with Scer\GAL4pnr-MD237 bunGD1392 ommatidium bunrI043 bunGE12327 ommatidium (with bunA-149B) bunGE12327 ommatidium (with bunA-Q578X) bunGE12327 ommatidium (with bunGE12327) bunA-Q578X bunA-149B ommatidium | somatic clone bunA-Q922X bunA-149B bun200B oocyte associated follicle cell | somatic clone bununspecified photoreceptor cell R3 (with bunA-Q578X) bunGE12327 photoreceptor cell R3 (with bunGE12327) bunA-Q578X photoreceptor cell R4 (with bunA-Q578X) bunGE12327 photoreceptor cell R4 (with bunGE12327) bunA-Q578X photoreceptor cell R7 bunrI043 photoreceptor cell R8 bunrI043 rhabdomere | somatic clone bunA-Q922X bunA-149B bun200B segment bun00255 trichogen cell, with Scer\GAL4pnr-MD237 bunGD1392 wing bunrI043 wing disc | somatic clone bunA-Q578X bunA-Q922X wing vein (with bunout4) bunrI043 wing vein (with bunout12) bunrI043 wing vein (with bunout14) bunrI043 wing vein (with bunrI043) bunout12 bunout14 bunout4
Classical Alleles ( 133 )
For All Classical Alleles Show
Allele of bun Class Mutagen Stocks Known lesion bunDG03606 2 -- bunDG12707 P-element activity 2 -- bunEY03766 P-element activity 2 -- bunNP7306 2 -- bun00255 P-element activity 1 -- bun06708 P-element activity 1 -- bun5-HA-1183 1 -- bun5-HA-1268 1 -- bun5-SZ-3100 1 -- bunBG01623 P-element activity 1 -- bunc00734 1 -- bunc01323 1 -- bunc04901 piggyBac transposase 1 -- bunc05328 piggyBac transposase 1 -- bunc06398 1 -- bunc06899 piggyBac transposase 1 -- bunCB-5579-3 1 -- bund00940 P-element activity 1 -- bund01435 1 -- bund05426 P-element activity 1 -- bund08534 P-element activity 1 -- bund10269 P-element activity 1 -- bund10473 P-element activity 1 -- bunDG02803 P-element activity 1 -- bune02988 piggyBac transposase 1 -- bune03005 piggyBac transposase 1 -- bune03971 1 -- bune04267 1 -- bune04666 piggyBac transposase 1 -- bunEY11024 P-element activity 1 -- bunf00045 1 -- bunf00153 piggyBac transposase 1 -- bunf02135 piggyBac transposase 1 -- bunf03933 piggyBac transposase 1 -- bunf07051 piggyBac transposase 1 -- bunk00612 P-element activity 1 -- bunk02903a P-element activity 1 Yes bunKG00392 P-element activity 1 -- bunKG00456 P-element activity 1 -- bunKG02858 P-element activity 1 -- bunKG07772 1 -- bunKG08095 1 -- bunMI00312 1 -- bunMI02374 1 -- bunNP0132 1 -- bunNP0147 1 -- bunNP0362 1 -- bunNP1108 1 -- bunNP2627 1 -- bunNP2669 1 -- bunNP3189 1 -- bunNP4760 1 -- bunNP5263 1 -- bunNP6230 1 -- bunNP6241 1 -- bunNP7332 1 -- bunNP7353 1 -- bunSwitch1.32 P-element activity 1 Yes bunUM-8365-3 1 -- bun01550 P-element activity 0 Yes bun02687 P-element activity 0 Yes bun03504 P-element activity 0 Yes bun04230 P-element activity 0 Yes bun05479 P-element activity 0 -- bun06475 P-element activity 0 -- bun06903 P-element activity 0 Yes bun07209 P-element activity 0 -- bun07692 P-element activity 0 Yes bun200B Delta2-3 0 Yes bun585RSX ethyl methanesulfonate 0 Yes bunA-149B Delta2-3 0 Yes bunA-211B Delta2-3 0 Yes bunA-P519L ethyl methanesulfonate 0 Yes bunA-Q578X ethyl methanesulfonate 0 Yes bunA-Q609X ethyl methanesulfonate 0 Yes bunA-Q879X ethyl methanesulfonate 0 Yes bunA-Q922X ethyl methanesulfonate 0 Yes bunA-Q988X ethyl methanesulfonate 0 Yes bunA-R508W ethyl methanesulfonate 0 Yes bunB-132A Delta2-3 0 Yes bunB-181A Delta2-3 0 Yes bunC-158B Delta2-3 0 Yes bunc03428 piggyBac transposase 0 -- bunc06946 piggyBac transposase 0 -- bunCB-6448-3 0 -- bunCB03431 P-element activity 0 Yes bunCPTI004088 piggyBac transposase 0 Yes bunCPTI004166 piggyBac transposase 0 Yes bunCPTI004174 piggyBac transposase 0 Yes bund02931 0 -- bund03667 0 -- bund05833 P-element activity 0 -- bund06337 P-element activity 0 -- bund06547 P-element activity 0 -- bund07483 P-element activity 0 -- bund08884 0 -- bunDP00598 0 -- bunEP2015 0 -- bunEP2194 0 -- bunEP2447 P-element activity 0 -- bunEP2622 P-element activity 0 -- bunEP488 P-element activity 0 -- bunEP830 P-element activity 0 -- bunG409 P-element activity 0 Yes bunGE11969 0 Yes bunGE12327 hypomorphic allele - genetic evidence 0 Yes bunGE12921 0 Yes bunGE14917 0 Yes bunk02603 P-element activity 0 -- bunk02903 0 -- bunk06713 P-element activity 0 -- bunk08813 P-element activity 0 -- bunk09018 P-element activity 0 -- bunk09036 P-element activity 0 Yes bunk10138 P-element activity 0 Yes bunk10318 P-element activity 0 Yes bunk14715 P-element activity 0 -- bunk16604 P-element activity 0 -- bunKG06590 P-element activity 0 -- bunNP4789 0 -- bunout12 Delta2-3 0 Yes bunout14 Delta2-3 0 Yes bunout4 Delta2-3 0 Yes bunP1.24 Delta2-3 0 Yes bunP2.7.03 0 -- bunrH541 P-element activity 0 -- bunrI043 P-element activity 0 -- bunrI43Δ15 Delta2-3 0 -- bunrI482 P-element activity 0 -- bunrK499 P-element activity 0 -- bunUG43 P-element activity 0 -- bununspecified 0 -- bunΔGE12327 Delta2-3 0 Yes
Alleles Carried on Transgenic Constructs ( 12 )
For All Alleles Carried on Transgenic Constructs Show
Allele of bun Class Mutagen Stocks Known lesion bunGD1392 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes bunGD10387 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes bunJF02954 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes bun1.Scer\UAS in vitro construct - regulatory fusion 0 Yes bun2.Scer\UAS in vitro construct - regulatory fusion 0 Yes bunA.Scer\UAS in vitro construct - regulatory fusion 0 Yes bunB.Scer\UAS in vitro construct - regulatory fusion 0 Yes bunC.Scer\UAS in vitro construct - regulatory fusion 0 Yes buncDNA1.GMR in vitro construct - regulatory fusion 0 Yes buncDNA2.GMR in vitro construct - regulatory fusion 0 Yes bunScer\UAS.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes bunX.Scer\UAS in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)BSC407 (Christensen et al., 2008.4.15) Df(2L)Exel6033 (Ryder, 2004.4.26) Df(2L)prd1.7 (BDGP Project Members, 1994-1999, Gluderer et al., 2008) Df(2L)Prl (Spradling et al., 1999, BDGP Project Members, 1994-1999, Gluderer et al., 2008)
Not disrupted in	Df(2L)esc10 (BDGP Project Members, 1994-1999, Kania et al., 1995) Df(2L)H20 (BDGP Project Members, 1994-1999) Df(2L)pr9201 (Butler, 2001) Df(2L)pr11163 (Butler, 2001) Df(2L)prd1.7 (Kania et al., 1995) Df(2L)TW50 (BDGP Project Members, 1994-1999) Df(2L)TW65 (Butler, 2001) In(2LR)TE35B-4LTE35B-14R (BDGP Project Members, 1994-1999)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD1392} NA P{TRiP.JF02954} NA P{UAS-bun.1} NA P{UAS-bun.2} NA P{UAS-bun.A} NA P{UAS-bun.B} NA P{UAS-bun.C} NA P{UAS-bun.X} NA P{UAS-GFP-bunA.attB} NA characterization construct P{GMR-shsclass1} NA P{GMR-shsclass2} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}bunEP488 NA P{EP}bunEP830 NA P{EP}bunEP2015 NA P{EP}bunEP2194 NA P{EP}bunEP2447 NA P{EP}bunEP2622 NA P{EP}bunGE11969 NA P{EP}bunGE12327 NA P{EP}bunGE12921 NA P{EP}bunGE14917 NA P{EPgy2}bunEY03766 NA P{EPgy2}bunEY11024 NA P{Mae-UAS.6.11}bunDP00598 NA P{Mae-UAS.6.11}bunP1.24 NA P{XP}bund00940 NA P{XP}bund01435 NA P{XP}bund02931 NA P{XP}bund03667 NA P{XP}bund05426 NA P{XP}bund05833 NA P{XP}bund06337 NA P{XP}bund06547 NA P{XP}bund07483 NA P{XP}bund08534 NA P{XP}bund08884 NA P{XP}bund10269 NA P{XP}bund10473 NA insertion of enhancer trap P{lacW}bunk00612 No P{lacW}bunk02603 No P{lacW}bunk02903a No P{lacW}bunk06713 No P{lacW}bunk08813 No P{lacW}bunk09018 No P{lacW}bunk09036 No P{lacW}bunk10138 No P{lacW}bunk10318 No P{lacW}bunk14715 No P{lacW}bunk16604 No P{lacW}l(2)SH0357SH0357 No P{lArB}bunUG43 No P{PZ}bun00255 No P{PZ}bun01550 No P{PZ}bun02687 No P{PZ}bun03504 No P{PZ}bun04230 No P{PZ}bun05479 No P{PZ}bun06475 No P{PZ}bun06708 No P{PZ}bun06903 No P{PZ}bun07209 No P{PZ}bun07692 No P{PZ}bunrH541 No P{PZ}bunrI043 No P{PZ}bunrI482 No P{PZ}bunrK499 No miscellaneous insertions Mi{MIC}bunMI00312 NA Mi{MIC}bunMI02374 NA P{?lacW}bunout4 NA P{?lacW}bunout12 NA P{?lacW}bunout14 NA P{GT1}bunBG01623 NA P{PTT-GB}bunCB03431 NA P{PTT-GB}bunG409 NA P{RS3}bunCB-5579-3 NA P{RS3}bunCB-6448-3 NA P{RS3}bunUM-8365-3 NA P{RS5}bun5-HA-1183 NA P{RS5}bun5-HA-1268 NA P{RS5}bun5-SZ-3100 NA P{SUPor-P}bunKG00392 NA P{SUPor-P}bunKG00456 NA P{SUPor-P}bunKG02858 NA P{SUPor-P}bunKG06590 NA P{SUPor-P}bunKG07772 NA P{SUPor-P}bunKG08095 NA P{Switch1}bunSwitch1.32 NA P{wHy}bunDG02803 NA P{wHy}bunDG03606 NA P{wHy}bunDG12707 NA PBac{806.LOX-SVS-2}bunCPTI004088 NA PBac{806.LOX-SVS-2}bunCPTI004166 NA PBac{806.LOX-SVS-2}bunCPTI004174 NA PBac{PB}bunc00734 NA PBac{PB}bunc01323 NA PBac{PB}bunc03428 NA PBac{PB}bunc04901 NA PBac{PB}bunc05328 NA PBac{PB}bunc06398 NA PBac{PB}bunc06899 NA PBac{PB}bunc06946 NA PBac{RB}bune02988 NA PBac{RB}bune03005 NA PBac{RB}bune03971 NA PBac{RB}bune04267 NA PBac{RB}bune04666 NA PBac{WH}bunf00045 NA PBac{WH}bunf00153 NA PBac{WH}bunf02135 NA PBac{WH}bunf03933 NA PBac{WH}bunf07051 NA insertion of enhancer trap binary system P{GawB}bunNP0132 No P{GawB}bunNP0147 No P{GawB}bunNP0362 No P{GawB}bunNP1108 No P{GawB}bunNP2627 No P{GawB}bunNP2669 No P{GawB}bunNP3189 No P{GawB}bunNP4760 No P{GawB}bunNP4789 No P{GawB}bunNP5263 No P{GawB}bunNP6230 No P{GawB}bunNP6241 No P{GawB}bunNP7306 No P{GawB}bunNP7332 No P{GawB}bunNP7353 No
Gene Ontology: Function, Process & Cellular Component ( 23 unique terms )
Terms Based on Experimental Evidence ( 14 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
autophagic cell death inferred from expression pattern (Gorski et al., 2003) chorion-containing eggshell formation inferred from mutant phenotype (Dobens et al., 2000) compound eye photoreceptor cell differentiation inferred from mutant phenotype (Treisman et al., 1995) decapentaplegic signaling pathway inferred from genetic interaction with dpp (Treisman et al., 1995) dorsal appendage formation inferred from mutant phenotype (Dobens et al., 1997) negative regulation of apoptosis inferred from mutant phenotype (Wu et al., 2008) negative regulation of cell fate specification inferred from mutant phenotype (Dobens et al., 2000) negative regulation of Notch signaling pathway inferred from mutant phenotype (Dobens et al., 2005) ovarian follicle cell migration inferred from mutant phenotype (Dobens et al., 2005) positive regulation of cell growth inferred from mutant phenotype (Wu et al., 2008, Gluderer et al., 2008) positive regulation of cell proliferation inferred from mutant phenotype (Wu et al., 2008) salivary gland cell autophagic cell death inferred from expression pattern (Gorski et al., 2003) segmentation inferred from mutant phenotype (Perrimon et al., 1996)
Cellular Component
CV term Evidence References
cytoplasm inferred from direct assay (Treisman et al., 1995)
Terms Based on Predictions or Assertions ( 11 terms )
Molecular Function
CV term Evidence References
protein homodimerization activity inferred from sequence or structural similarity (Dobens et al., 1997) sequence-specific DNA binding transcription factor activity inferred from electronic annotation with InterPro:IPR000580 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
cell fate determination non-traceable author statement (Swiss-Prot Project Members, 1997.2.1, Swiss-Prot Project Members, 2002.3.1) compound eye development non-traceable author statement (Swiss-Prot Project Members, 1997.2.1, Swiss-Prot Project Members, 2002.3.1) dorsal appendage formation non-traceable author statement (Swiss-Prot Project Members, 1997.2.1) oogenesis non-traceable author statement (Swiss-Prot Project Members, 1997.2.1) ovarian follicle cell development traceable author statement (Dobens and Raftery, 2000) peripheral nervous system development non-traceable author statement (Swiss-Prot Project Members, 1997.2.1, Prokopenko et al., 2000, Swiss-Prot Project Members, 2002.3.1) regulation of transcription, DNA-dependent inferred from electronic annotation with InterPro:IPR000580 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
cytoplasm non-traceable author statement (Swiss-Prot Project Members, 2002.3.1) nucleus non-traceable author statement (Swiss-Prot Project Members, 1997.2.1)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions bun allele Gene References bun02687 Kr (Abrell et al., 2000) bun05479 Kr (Abrell et al., 2000) bun06475 Kr (Abrell et al., 2000) bun06903 aop (Treisman et al., 1995) bunEP488 pnr (Pena-Rangel et al., 2002) bunrI043 aop (Treisman et al., 1995) hh (Treisman et al., 1995) S (Treisman et al., 1995) unspecified dpp (Treisman et al., 1995) os (Mukherjee et al., 2006)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 60711 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0259176 DroID - A comprehensive database of gene and protein interactions. • FBgn0259176 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 34665
Orthologs
Genome-wide drosophilid orthologs	Dana\GF15669 Dana\GF19725 Dere\GG10251 Dere\GG10256 Dgri\GH11670 Dgri\GH11674 Dmoj\GI17114 Dper\GL18765 Dper\GL18768 Dpse\GA16527 Dpse\GA25789 Dsec\GM26119 Dsec\GM26174 Dsim\GD22111 Dsim\GD22116 Dvir\GJ16158 Dwil\GK14621 Dwil\GK14623 Dyak\GE12185 Dyak\GE12250
Curated drosophilid orthologs	Dpse\GA16527   Dpse\GA18896
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AGAP010044-PA Anopheles gambiae (mosquito) XP_395024.3 Apis mellifera (honey bee) XP_001947979.1 Acyrthosiphon pisum (pea aphid) BGIBMGA002705-PA Bombyx mori (silk worm) ENSBTAP00000024485 Bos taurus (bovine) ENSCAFP00000012330 Canis familiaris (dog) ENSCINP00000014707 Ciona intestinalis (sea squirt) ENSCPOP00000001337 Cavia porcellus (guinea pig) RP29468 Caenorhabditis remanei (nematode) ENSECAP00000003558 Equus caballus (horse) ENSGACP00000012055 Gymnopilus aculeatus (mushroom) ENSGALP00000035712 Gallus gallus (chicken) ENSP00000354543 Homo sapiens (human) ENSMODP00000026588 Monodelphis domestica (gray opossum) ENSMMUP00000023274 Macaca mulatta (rhesus monkey) ENSMUSP00000096688 Mus musculus (house mouse) ENSORLP00000003994 Oryzias latipes (japanese killifish) PHUM011571-PA Pediculus humanus (human body louse) ENSPPYP00000015886 Pongo pygmaeus (orangutan) ENSPTRP00000009932 Pan troglodytes (chimpanzee) ENSRNOP00000001363 Rattus norvegicus (norway rat) ENSTNIP00000010630 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000046148 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000043387 Xenopus tropicalis (frog)
Stocks & Reagents
Stocks Listed in FlyBase ( 67 )
Harvard	- P{XP}bund00940 - P{XP}bund01435 - P{XP}bund05426 - P{XP}bund08534 - P{XP}bund10269 - P{XP}bund10473 - PBac{PB}bunc00734 - PBac{PB}bunc01323 - PBac{PB}bunc04901 - PBac{PB}bunc05328 - PBac{PB}bunc06398 - PBac{PB}bunc06899
	More stocks available...
Genomic Clones ( 3 )
	BACR05E09 BACR28A12 BACR48M09 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 197 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	AT11610 GH13775 IP08092
Other clones	AF171800 DPiM_GH13775
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH05432 HL02678 HL03125 HL05759 LP01472 LP08578 RE02207 RE09733 RE13976 RE27537 RE30963 RE30987 RE31763 RE34255 RE35296 RE40171 RE44889 RE47478 RE49084 RE54843 RE55078 RE55730 RE60759 RH67674 RH68186 SD12839
Other clones	7396 9842 21126 44781 56972 59764 65776 69755 75501 82441 100968 101615 108436 108716 119132 119404 123696 145649 145748 146168 149357 163212 174834 191249 193223 214251.19 283580 296185 534027 569700 bs26b10 CK01.48D.D3 CK01.58D.G5 CK01.89A.E4 CK01.89D.D11 CK01.106A.G5 CK01.129B.D7 CK01.132B.D6 CK01.145B.E2 Dmel_neb_011469_0386_1940 Dmel_neb_011962_0332_2497 EC053674 EC056648 EC258011 EK040914 EK047727 EK067909 EK070050 EK070714 EK088527 EK095147 EK095716 EK097922 EK102447 EK108912 EK119449 EK125445 EK154301 EK160241 EK164409 EK170357 EK171604 EK172614 EK182002 EK185501 EK186330 EK193119 EK197053 EK205250 EK210705 EK212501 EK215837 EK223448 EK228863 EK234654 EK238714 EK239150 EK246554 EK248358 EK248563 EK249142 EK250222 EK254650 EK255008 EK267424 EK294029 EK295803 EK296446 EK308828 EN02725 EN09620 EN10054 EN14238 EP11904 EP13231 EP15305 ESG012a.E21_A02 ESG019c.E21_C03 ESG0111c.B21_H06 ESG0112d.B21_A01 ESG0115b.B21_A07 ESG0115d.B21_D10 ESG0116c.B21_B08 ESG0117a.B21_E04 FGA007H02 FGC009D08 FGC011H02 FGC020H10 FGC028E08 FGH124G01 FGM002B11 FGM101A09 FGM308E02 FGM321D03 IP08192 IP08292 IP11852 RE01782 RH08983 RP003032316 RP003041926 RP003055985 RP003079049 RP003107724 RP003120021 RP003134756 RP003141841 RP003155642 RP003166430 RP003187135 RP003197512 RP003205061 RP003206224 RP003218115 RP003220885 RP003221526 RP003229734 RP003264332 RP003269305 RP003282381 RP003288667 RP003301506 RP003315573 RP003317410 RP003334371 RP003337278 RP003340632 RP003353234 RP003383237 RP003390242 RP003400075 RP003405584 RP003416963 RP003428670 RP003459696 RP003500754 RP003535570 RP003549420 RP003550961 RP003557068 RP003616320 RP003629819 RP003644366 RP003670186 RP003672023 RP003681402
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0259176 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 34665 318985
Antibody Information
	monoclonal (Treisman et al., 1995) polyclonal (Kim et al., 2009)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: bun CG5461 (Zhong, 1999.11)
Source for database merge of	Source for merge of: bun CG31857 (FlyBase Genome Annotators, 2008)
Additional comments	Annotations CG5461 and CG31857 merged as CG42281 in release 5.8 of the genome annotation. (FlyBase Genome Annotators, 2008)
Other Comments
	bun regulates the level of N stimulation to position the boundary between centripetally migrating and stationary columnar cells. (Dobens et al., 2005) New annotation (CG31857) in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003) bun integrates opposing dpp and EGF signals to set the operculum boundary in the developing egg. (Dobens et al., 2000) Identification: Enhancer trap expression pattern survey for loci expressed in the ring gland. (Harvie et al., 1998) bun has been cloned and sequenced. (Dobens et al., 1997) The autosomal "FLP-DFS" technique (using the P{ovoD1-18} P{FRT(whs)} P{hsFLP} chromosomes) has been used to identify the specific maternal effect phenotype for the zygotic lethal mutation. (Perrimon et al., 1996) Mutations in bun cause a delay in photoreceptor neural differentiation relative to dpp expression suggesting that bun may be required for the transmission of the dpp signal. bun has a role in inducing rotation of the second optic lobe. bun encodes a cytoplasmic protein expressed anterior to the furrow in a hh-dependent manner. (Treisman et al., 1995)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_080364 NM_165012 NM_165013 NM_001042892 NM_001042893 NM_001042894 NM_001169493 NM_001169494 RefSeq (Proteins) • NP_525103 NP_723755 NP_723756 NP_001036357 NP_001036358 NP_001036359 NP_001162964 NP_001162965 DDBJ / EMBL / GenBank • AA697769 AAB61239 AAC41607 AAC41608 AAF53200 AAF53201 AAL25334 AAN10817 ABI31311 ABI31312 ABI31313 ACZ94250 ACZ94251 AF003342 AI135873 AI513268 AI946452 AQ024970 AQ026018 AQ034170 AQ073593 AQ073735 AY060295 BH011405 BH759209 BH759229 BH840697 BH854538 CL844389 CZ468646 CZ469597 CZ469883 CZ469884 CZ471066 CZ471108 CZ471907 CZ474523 CZ474750 CZ475062 CZ475201 CZ475762 CZ475763 CZ476281 CZ477188 CZ477189 CZ477276 CZ480758 CZ480770 CZ482440 CZ482441 CZ482640 CZ484634 CZ486537 CZ489808 G00410 L42511 L42512 Entrez Gene - A searchable database of RefSeq genes. • 34665 UniProtKB/Swiss-Prot • Q24522 Q24523 UniProtKB/TrEMBL • E1JH96 E1JHG7
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • TSC-22 / Dip / Bun (IPR000580)
Linkouts
	BioGRID - A database of protein and genetic interactions • 60711 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0259176 DroID - A comprehensive database of gene and protein interactions. • FBgn0259176 DRSC - Results from RNAi screens. • FBgn0259176 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0259176 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG31857-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0259176 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 34665 318985 InParanoid A subset of ortholog calls from InParanoid. • AGAP010044-PA Anopheles gambiae (mosquito) XP_395024.3 Apis mellifera (honey bee) XP_001947979.1 Acyrthosiphon pisum (pea aphid) BGIBMGA002705-PA Bombyx mori (silk worm) ENSBTAP00000024485 Bos taurus (bovine) ENSCAFP00000012330 Canis familiaris (dog) ENSCINP00000014707 Ciona intestinalis (sea squirt) ENSCPOP00000001337 Cavia porcellus (guinea pig) RP29468 Caenorhabditis remanei (nematode) ENSECAP00000003558 Equus caballus (horse) ENSGACP00000012055 Gymnopilus aculeatus (mushroom) ENSGALP00000035712 Gallus gallus (chicken) ENSP00000354543 Homo sapiens (human) ENSMODP00000026588 Monodelphis domestica (gray opossum) ENSMMUP00000023274 Macaca mulatta (rhesus monkey) ENSMUSP00000096688 Mus musculus (house mouse) ENSORLP00000003994 Oryzias latipes (japanese killifish) PHUM011571-PA Pediculus humanus (human body louse) ENSPPYP00000015886 Pongo pygmaeus (orangutan) ENSPTRP00000009932 Pan troglodytes (chimpanzee) ENSRNOP00000001363 Rattus norvegicus (norway rat) ENSTNIP00000010630 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000046148 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000043387 Xenopus tropicalis (frog) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /gene/shortsit.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 34665 modMine - Data generated by the modENCODE project. • FBgn0259176
Synonyms & Secondary IDs ( 24 )
Reported As
Symbol Synonym	1550 (Harvie et al., 1998) bnc (Wang et al., 2004) bun (Blanco et al., 2010, Aerts et al., 2007, Ash et al., 2007, Neuman-Silberberg, 2007, Maines et al., 2007, Luo et al., 2011, Christensen et al., 2008.4.15, Wu et al., 2007, Biemar et al., 2006, Mukherjee et al., 2006, Gluderer et al., 2007, Buszczak et al., 2007, Quinones-Coello, 2007, Gan et al., 2010, FlyBase Curators, 2008, Levine et al., 2007, Wu et al., 2008, Gluderer et al., 2008, Yamamoto et al., 2009.2.25, Liu and Lehmann, 2008, Gluderer et al., 2009.02.09, Spradling et al., 2011) Bun (Martin et al., 2007) BunA (Gluderer et al., 2010) CG5461 (Lee et al., 2003, Whitfield, 2001.1.5, Quinones-Coello, 2007, Perkins et al., 2009) CG31857   CG42281 (FlyBase Genome Annotators, 2008) EP(2)0488 (Pena-Rangel et al., 2002) l(2)00255 (Russell et al., 1998, Perrimon et al., 1996, ) l(2)02687 (Abrell et al., 2000) l(2)05479 (Abrell et al., 2000) l(2)06475 (Abrell et al., 2000) l(2)k02903 (Butler, 2001) l(2)rI043   shs (Treisman, 1995.9.29, Treisman, 1995.9.29, Dobens and Raftery, 1997, Moses and Serra, 1996, Huang and Kunes, 1996, Dobens and Raftery, 1996, Treisman et al., 1995, ) TSC-22 (Dobens et al., 1995, Gluderer et al., 2010) Tsc22   unnamed (Treisman and Rubin, 1996)
Name Synonym	bunched (Ash et al., 2007, Neuman-Silberberg, 2007, Dobens, 1997.5.8, Wu et al., 2007, Dobens and Levine, 2006, Biemar et al., 2006, Arbouzova and Zeidler, 2006, Levine et al., 2007, Wu et al., 2008, Gluderer et al., 2008, Kim et al., 2009, Liu and Lehmann, 2008, Carpenter et al., 2009, Wang et al., 2004, Levine et al., 2010, Spradling et al., 2011) Bunched (Martin et al., 2007, Gluderer et al., 2010) CG31857   CG42281 (FlyBase Genome Annotators, 2008) short-sighted (Thummel and Chory, 2002) shortsighted (White et al., 1999) short sighted
Secondary FlyBase IDs
	FBgn0010460 FBgn0010670 FBgn0011724 FBgn0014385 FBgn0051857
References ( 115 )
Generate a list of	All references relating to this gene ( 115 )
List References by type	Research paper  ( 47 ) Supplementary material  ( 6 ) Review  ( 5 ) Personal communication to FlyBase  ( 18 ) Abstract  ( 23 ) FlyBase analysis  ( 4 ) DNA/RNA sequence record  ( 3 ) Curated genome annotation  ( 2 ) Computer file  ( 4 ) Protein sequence record  ( 2 ) Automatic genome annotation  ( 1 )
Recent research papers ( 7 )
	Carreira et al., 2011, BMC Dev. Biol. 11: 32 Genetic basis of wing morphogenesis in Drosophila: sexual dimorphism and non-allometric effects of shape variation. [FBrf0214273] Luo et al., 2011, Development 138(13): 2761--2771 Direct targets of the D. melanogaster DSXF protein and the evolution of sexual development. [FBrf0213878] Spradling et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(38): 15948--15953 Drosophila P elements preferentially transpose to replication origins. [FBrf0215815] Blanco et al., 2010, BMC Dev. Biol. 10: 94 Gene expression following induction of regeneration in Drosophila wing imaginal discs. Expression profile of regenerating wing discs. [FBrf0211812] Gan et al., 2010, Cell Res. 20(7): 763--783 Dynamic regulation of alternative splicing and chromatin structure in Drosophila gonads revealed by RNA-seq. [FBrf0211191] Gluderer et al., 2010, J. Biol. 9(1): 9 Madm (Mlf1 adapter molecule) cooperates with Bunched A to promote growth in Drosophila. [FBrf0210782] Levine et al., 2010, Dev. Biol. 344(1): 196--209 Opposing interactions between Drosophila Cut and the C/EBP encoded by Slow Border Cells direct apical constriction and epithelial invagination. [FBrf0211314] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...