General Information
Symbol
Dmel\cno
Species
D. melanogaster
Name
canoe
Annotation Symbol
CG42312
Feature Type
FlyBase ID
FBgn0259212
Gene Model Status
Stock Availability
Gene Snapshot
Canoe functions as a scaffold protein in adherens junctions and is involved in morphogenesis in a variety of tissues. [Date last reviewed: 2016-06-16]
Also Known As
Canoe, lip
Genomic Location
Cytogenetic map
Sequence location
3R:5,170,004..5,217,456 [-]
Recombination map
3-47.5
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
GO Summary Ribbons
Families, Domains and Molecular Function
Gene Group Membership (FlyBase)
Protein Family (UniProt, Sequence Similarities)
-
Molecular Function (see GO section for details)
Summaries
Phenotypic Description from the Red Book (Lindsley and Zimm 1992)
cno: canoe
Homozygous lethal; dorsal surface of embryo open.
Interactive Fly
glgf/dhr motif scaffolding protein - interacts with genes in both the Notch and the Ras pathways - in a complex with Pins, Canoe contributes to proper Mud localization and spindle orientation, as well as to the correct asymmetric distribution of cell-fate determinants in metaphase NBs
Gene Model and Products
Number of Transcripts
8
Number of Unique Polypeptides
7

Please see the GBrowse view of Dmel\cno or the JBrowse view of Dmel\cno for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Gene model reviewed during 5.47
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
No evidence for 13aa QQQRYLGQSLPAE from SWP:Q24279 in the 18th exon of this gene.
Gene model reviewed during 6.03
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0299709
7597
1882
FBtr0299710
6252
1817
FBtr0300525
6987
2051
FBtr0334910
5707
1721
FBtr0334911
6687
1951
FBtr0334912
6738
1968
FBtr0334913
9118
1818
FBtr0347363
10585
2051
Additional Transcript Data and Comments
Reported size (kB)
8.2, 7.5, 7.0 (northern blot)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0288987
208.9
1882
7.83
FBpp0288988
200.5
1817
7.78
FBpp0289752
226.9
2051
7.23
FBpp0306931
190.0
1721
7.19
FBpp0306932
216.3
1951
7.62
FBpp0306933
218.1
1968
7.06
FBpp0306934
200.6
1818
7.74
FBpp0312543
226.9
2051
7.23
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

2051 aa isoforms: cno-PE, cno-PJ
Additional Polypeptide Data and Comments
Reported size (kDa)
1893 (aa); 200 (kD observed)
Comments
External Data
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\cno using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (24 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
inferred from physical interaction with FLYBASE:Ran; FB:FBgn0020255
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000044747
(assigned by GO_Central )
Biological Process (15 terms)
Terms Based on Experimental Evidence (10 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:Ran; FB:FBgn0020255
inferred from genetic interaction with FLYBASE:pins; FB:FBgn0040080
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:bsk; FB:FBgn0000229
inferred from direct assay
inferred from mutant phenotype
Terms Based on Predictions or Assertions (8 terms)
CV Term
Evidence
References
non-traceable author statement
traceable author statement
non-traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN000044747
(assigned by GO_Central )
Cellular Component (5 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
inferred from direct assay
colocalizes_with adherens junction
inferred from direct assay
inferred from direct assay
inferred from high throughput direct assay
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
non-traceable author statement
traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN000044747
(assigned by GO_Central )
traceable author statement
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
epithelial cell

Comment: within polygonal cortical network

epithelium

Comment: within polygonal cortical network

northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
In wild type embryos, cno transcripts are expressed in dorsal-ventral oriented rows of cells in the posterior portion of each thoracic and abdominal segment. cno was shown to be induced by ubiquitous Ubx expression. cno expression in various homeotic mutant backgrounds was also described.
cno transcripts are most abundant in embryos, decrease in larvae, and increase again in pupae and adults. The relative abundance of the three transcript species varies with developmental stage. In early stage 5 embryos, a low uniform level of cno transcripts is seen. At the cellular blastoderm stage, expression is seen along the dorsal midline and in three ectodermal stripes in the central region of the embryo. During stages 7-10, expression is confined to the dorsal furrows and the posterior midgut rudiment. Ectodermal expression becomes evident in stage 10. In stage 13, focal stainings are detected near the attachment site of the midgut with the foregut and hindgut. In eye discs, cno is expressed ubiquitously with a higher concentration of transcripts in the lateral edge region.
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immuno-electron microscopy
Stage
Tissue/Position (including subcellular localization)
Reference
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
cno protein is localized to the adherens junctions of neuroepithelial cell in the larval inner and outer optic anlagen.
Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
colocalizes_with adherens junction
inferred from direct assay
inferred from direct assay
inferred from high throughput direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\cno in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 55 )
For All Classical and Insertion Alleles Show
 
Allele of cno
Class
Mutagen
Associated Insertion
Stocks
Known lesion
    0
    --
      0
      --
        0
        --
          0
          --
            0
            --
              0
              --
                0
                --
                  0
                  --
                  Other relevant insertions
                  Transgenic Constructs ( 16 )
                  For All Alleles Carried on Transgenic Constructs Show
                  Transgenic constructs containing/affecting coding region of cno
                  Allele of cno
                  Mutagen
                  Associated Transgenic Construct
                  Stocks
                  Transgenic constructs containing regulatory region of cno
                  Deletions and Duplications ( 7 )
                  Phenotypes
                  For more details about a specific phenotype click on the relevant allele symbol.
                  Lethality
                  Allele
                  Other Phenotypes
                  Allele
                  Phenotype manifest in
                  Allele
                  macrochaeta & postpronotum
                  macrochaeta & scutellum
                  macrochaeta & scutum
                  macrochaeta & wing
                  rhabdomere R1 & microvillus
                  rhabdomere R4 & microvillus
                  Orthologs
                  Human Orthologs (via DIOPT v7.1)
                  Homo sapiens (Human) (3)
                  Species\Gene Symbol
                  Score
                  Best Score
                  Best Reverse Score
                  Alignment
                  Complementation?
                  Transgene?
                  12 of 15
                  Yes
                  Yes
                   
                  1 of 15
                  No
                  Yes
                  1 of 15
                  No
                  Yes
                  Model Organism Orthologs (via DIOPT v7.1)
                  Mus musculus (laboratory mouse) (3)
                  Species\Gene Symbol
                  Score
                  Best Score
                  Best Reverse Score
                  Alignment
                  Complementation?
                  Transgene?
                  10 of 15
                  Yes
                  Yes
                  1 of 15
                  No
                  Yes
                  1 of 15
                  No
                  Yes
                  Rattus norvegicus (Norway rat) (2)
                  9 of 13
                  Yes
                  Yes
                  1 of 13
                  No
                  Yes
                  Xenopus tropicalis (Western clawed frog) (2)
                  4 of 12
                  Yes
                  Yes
                  1 of 12
                  No
                  Yes
                  Danio rerio (Zebrafish) (3)
                  6 of 15
                  Yes
                  Yes
                  1 of 15
                  No
                  Yes
                  1 of 15
                  No
                  Yes
                  Caenorhabditis elegans (Nematode, roundworm) (1)
                  12 of 15
                  Yes
                  Yes
                  Arabidopsis thaliana (thale-cress) (0)
                  No orthologs reported.
                  Saccharomyces cerevisiae (Brewer's yeast) (1)
                  1 of 15
                  Yes
                  No
                  Schizosaccharomyces pombe (Fission yeast) (0)
                  No orthologs reported.
                  Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091900ET )
                  Organism
                  Common Name
                  Gene
                  AAA Syntenic Ortholog
                  Multiple Dmel Genes in this Orthologous Group
                  Drosophila melanogaster
                  fruit fly
                  Drosophila suzukii
                  Spotted wing Drosophila
                  Drosophila simulans
                  Drosophila sechellia
                  Drosophila erecta
                  Drosophila yakuba
                  Drosophila ananassae
                  Drosophila pseudoobscura pseudoobscura
                  Drosophila persimilis
                  Drosophila willistoni
                  Drosophila virilis
                  Drosophila mojavensis
                  Drosophila grimshawi
                  Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915009Q )
                  Organism
                  Common Name
                  Gene
                  Multiple Dmel Genes in this Orthologous Group
                  Musca domestica
                  House fly
                  Glossina morsitans
                  Tsetse fly
                  Lucilia cuprina
                  Australian sheep blowfly
                  Lucilia cuprina
                  Australian sheep blowfly
                  Mayetiola destructor
                  Hessian fly
                  Mayetiola destructor
                  Hessian fly
                  Aedes aegypti
                  Yellow fever mosquito
                  Anopheles darlingi
                  American malaria mosquito
                  Anopheles gambiae
                  Malaria mosquito
                  Culex quinquefasciatus
                  Southern house mosquito
                  Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W007R )
                  Organism
                  Common Name
                  Gene
                  Multiple Dmel Genes in this Orthologous Group
                  Bombyx mori
                  Silkmoth
                  Danaus plexippus
                  Monarch butterfly
                  Heliconius melpomene
                  Postman butterfly
                  Apis florea
                  Little honeybee
                  Apis mellifera
                  Western honey bee
                  Bombus impatiens
                  Common eastern bumble bee
                  Bombus terrestris
                  Buff-tailed bumblebee
                  Linepithema humile
                  Argentine ant
                  Megachile rotundata
                  Alfalfa leafcutting bee
                  Nasonia vitripennis
                  Parasitic wasp
                  Dendroctonus ponderosae
                  Mountain pine beetle
                  Tribolium castaneum
                  Red flour beetle
                  Pediculus humanus
                  Human body louse
                  Rhodnius prolixus
                  Kissing bug
                  Cimex lectularius
                  Bed bug
                  Acyrthosiphon pisum
                  Pea aphid
                  Zootermopsis nevadensis
                  Nevada dampwood termite
                  Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X007B )
                  Organism
                  Common Name
                  Gene
                  Multiple Dmel Genes in this Orthologous Group
                  Strigamia maritima
                  European centipede
                  Ixodes scapularis
                  Black-legged tick
                  Stegodyphus mimosarum
                  African social velvet spider
                  Tetranychus urticae
                  Two-spotted spider mite
                  Tetranychus urticae
                  Two-spotted spider mite
                  Tetranychus urticae
                  Two-spotted spider mite
                  Daphnia pulex
                  Water flea
                  Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G00CA )
                  Organism
                  Common Name
                  Gene
                  Multiple Dmel Genes in this Orthologous Group
                  Strongylocentrotus purpuratus
                  Purple sea urchin
                  Strongylocentrotus purpuratus
                  Purple sea urchin
                  Strongylocentrotus purpuratus
                  Purple sea urchin
                  Strongylocentrotus purpuratus
                  Purple sea urchin
                  Ciona intestinalis
                  Vase tunicate
                  Ciona intestinalis
                  Vase tunicate
                  Gallus gallus
                  Domestic chicken
                  Human Disease Model Data
                  FlyBase Human Disease Model Reports
                  Alleles Reported to Model Human Disease (Disease Ontology)
                  Download
                  Models ( 0 )
                  Allele
                  Disease
                  Evidence
                  References
                  Interactions ( 0 )
                  Allele
                  Disease
                  Interaction
                  References
                  Comments ( 0 )
                   
                  Human Orthologs (via DIOPT v7.1)
                  Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
                  Homo sapiens (Human)
                  Gene name
                  Score
                  OMIM
                  OMIM Phenotype
                  Complementation?
                  Transgene?
                  Functional Complementation Data
                  Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
                  Interactions
                  Summary of Physical Interactions
                  esyN Network Diagram
                  Show neighbor-neighbor interactions:
                  Select Layout:
                  Legend:
                  Protein
                  RNA
                  Selected Interactor(s)
                  Interactions Browser

                  Please look at the Interaction Group reports for full details of the physical interactions
                  protein-protein
                  Interacting group
                  Assay
                  References
                  Summary of Genetic Interactions
                  esyN Network Diagram
                  esyN Network Key:
                  Suppression
                  Enhancement

                  Please look at the allele data for full details of the genetic interactions
                  Starting gene(s)
                  Interaction type
                  Interacting gene(s)
                  Reference
                  Starting gene(s)
                  Interaction type
                  Interacting gene(s)
                  Reference
                  External Data
                  Linkouts
                  BioGRID - A database of protein and genetic interactions.
                  DroID - A comprehensive database of gene and protein interactions.
                  InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
                  Pathways
                  Gene Group - Pathway Membership (FlyBase)
                  External Data
                  Linkouts
                  SignaLink - A signaling pathway resource with multi-layered regulatory networks.
                  Genomic Location and Detailed Mapping Data
                  Chromosome (arm)
                  3R
                  Recombination map
                  3-47.5
                  Cytogenetic map
                  Sequence location
                  3R:5,170,004..5,217,456 [-]
                  FlyBase Computed Cytological Location
                  Cytogenetic map
                  Evidence for location
                  82F4-82F4
                  Limits computationally determined from genome sequence between P{PZ}l(3)0273302733 and P{EP}EP974
                  Experimentally Determined Cytological Location
                  Cytogenetic map
                  Notes
                  References
                  82E-82F
                  (determined by in situ hybridisation)
                  Experimentally Determined Recombination Data
                  Left of (cM)
                  Right of (cM)
                  Notes
                  Stocks and Reagents
                  Stocks (33)
                  Genomic Clones (44)
                  cDNA Clones (56)
                   

                  Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

                  cDNA clones, fully sequences
                  BDGP DGC clones
                  Other clones
                  Drosophila Genomics Resource Center cDNA clones

                  For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

                  cDNA Clones, End Sequenced (ESTs)
                  RNAi and Array Information
                  Linkouts
                  DRSC - Results frm RNAi screens
                  GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
                  Antibody Information
                  Laboratory Generated Antibodies
                   
                  polyclonal (species: Rat; amino acids 729-1171 were used to generate antibody)
                  Commercially Available Antibodies
                   
                  Other Information
                  Relationship to Other Genes
                  Source for database identify of
                  Source for identity of: cno CG2534
                  Source for database merge of
                  Source for merge of: lip cno
                  Source for merge of: cno CG31537
                  Source for merge of: cno anon-WO0172774.47
                  Additional comments
                  snRNA:U4atac:82E is encoded in an intron of cno.
                  Annotations CG2534 and CG31537 merged as CG42312 in release 5.9 of the genome annotation.
                  "S(CycEJP)68S10" is unlikely to correspond to "cno"; mutations in the two loci complement each other.
                  Source for merge of cno anon-WO0172774.47 was sequence comparison ( date:051113 ).
                  Eleven further alleles (all EMS) discarded by Tubingen.
                  Other Comments
                  cno is required for normal proneural wave progression.
                  A Ser-N-cno complex mediates essential interactions between glia and neuroepithelial cells in optic lobe development.
                  cno regulates linkage between adherens junctions and the actin cytoskeleton during morphogenesis.
                  RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
                  R and cno proteins act in the same molecular pathway during dorsal closure and the function of both proteins in dorsal closure depends on their ability to interact with each other. R acts upstream of cno in dorsal closure, but unlike cno, is not involved in the stimulation of JNK pathway activity.
                  dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
                  New annotation (CG31537) in release 3 of the genome annotation.
                  Both loss-of-function mutations of cno and overexpression of cno result in malformation of the rhabdomeres in the ommatidia.
                  Mutants are isolated in an EMS mutagenesis screen to identify zygotic mutations affecting germ cell migration at discrete points during embryogenesis: mutants exhibit pattern defects (dorsal open).
                  cno protein binds directly to pyd protein.
                  cno is involved in the JNK pathway for dorsal closure in embryos, as a modulator acting upstream of or in parallel with Rac1.
                  The mechanism by which cno product controls cone cell formation in the developing compound eye is studied.
                  lips is likely to be cno based on sequence identity and sequence of a nonsense cno mutation.
                  Isolated during a subtractive hybridisation protocol to identify Ubx-activated target genes. Ubx inducible during embryogenesis.
                  Genetic interactions suggest that cno participates with members of the N pathway in regulating adhesive cell-cell interactions for the determination of cell fate.
                  cno mutants display failure of dorsal closure.
                  Origin and Etymology
                  Discoverer
                  Etymology
                  Identification
                  External Crossreferences and Linkouts ( 87 )
                  Sequence Crossreferences
                  NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                  GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                  RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                  UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                  Linkouts
                  BioGRID - A database of protein and genetic interactions.
                  Drosophila Genomics Resource Center - Drosophila Genomics Resource Center cDNA clones
                  DroID - A comprehensive database of gene and protein interactions.
                  DRSC - Results frm RNAi screens
                  Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                  FLIGHT - Cell culture data for RNAi and other high-throughput technologies
                  FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                  FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                  Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                  Flygut - An atlas of the Drosophila adult midgut
                  FlyMine - An integrated database for Drosophila genomics
                  GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
                  iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                  Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
                  InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
                  KEGG Genes - Molecular building blocks of life in the genomic space.
                  MIST (genetic) - An integrated Molecular Interaction Database
                  MIST (protein-protein) - An integrated Molecular Interaction Database
                  modMine - A data warehouse for the modENCODE project
                  SignaLink - A signaling pathway resource with multi-layered regulatory networks.
                  Synonyms and Secondary IDs (19)
                  Reported As
                  Secondary FlyBase IDs
                  • FBgn0000340
                  • FBgn0000465
                  • FBgn0005637
                  • FBgn0046384
                  • FBgn0051537
                  Datasets (0)
                  Study focus (0)
                  Experimental Role
                  Project
                  Project Type
                  Title
                  References (200)