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Gene Dmel\C3G

General Information
Symbol	Dmel\C3G	Species	D. melanogaster
Name	C3G	Annotation symbol	CG42328
Feature type	protein_coding_gene	FlyBase ID	FBgn0259228
Gene Model Status	Current	Stock availability	11 publicly available
Also Known As	CG3126, C(3)G, DC3G
Genomic Location
Chromosome (arm)	X	Recombination map
Cytogenetic map	6D2-6D2	Sequence location	X:6,675,449..6,697,579 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene C3G is referred to in FlyBase by the symbol Dmel\C3G (CG42328, FBgn0259228). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: Ras guanyl-nucleotide exchange factor activity. There is experimental evidence that it is involved in the biological process: Ras protein signal transduction; somatic muscle development; sarcomere organization; muscle attachment; regulation of Rap GTPase activity. 15 alleles are reported. The phenotypes of these alleles are annotated with: organ system subdivision; external compound sense organ; organ system; adult segment; thoracic segment; peripheral nervous system; cell part; embryonic/larval hypodermal muscle; pigment cell; rhabdomere; adult; imaginal precursor. It has 6 annotated transcripts and 6 annotated polypeptides. Protein features are: Guanine-nucleotide dissociation stimulator CDC25; Ras guanine nucleotide exchange factor; Ras guanine nucleotide exchange factor, domain; Ras guanine-nucleotide exchange factor, conserved site; Ras-like guanine nucleotide exchange factor, N-terminal. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 12-18 embryonic stages, during late pupal stages. Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, larval/adult central nervous system, adult crop, adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, adult salivary gland, larval trachea. Gene sequence location is X:6675449..6697579.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2011_10	References Bakal et al., 2007, Science 316(5832): 1753--1756
FB2012_01	Sequence features HFA17745 BKN21885 BKN41974 HFA17929
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 6D2-6D2   Limits computationally determined from genome sequence between P{EP}C3GEP1613 and P{EP}EP1388EP1388&P{EP}EP1573
Experimentally Determined Cytological Location
Cytogenetic map Notes References 6C7-6D2     (Ishimaru et al., 1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\C3G for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene merge based on EST/cDNA data Due to an oversight in the implementation of gene annotation merges, the symbols for some of the transcripts and polypeptides of this gene were changed to non-unique designations in r5.9. Although there were no conflicts within a given release, the same symbols were used for different isoforms in different releases. The affected gene product symbols have now been changed to symbols not used previously in the database; see individual transcript reports for details.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) C3G-RC FBtr0299791  NM_176694   6880   1520 C3G-RD FBtr0299792  NM_001144694   5397   1285 C3G-RE FBtr0299793  NM_001144695   5632   1417 C3G-RF FBtr0299794  NM_001144696   5785   1468 C3G-RG FBtr0299795  NM_001144697   5244   1234 C3G-RH FBtr0300526  NM_132122   7033   1571
Additional Transcript Data & Comments
Reported size (kB)	7.0 (northern blot) (Ishimaru et al., 1999)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein C3G-PC   FBpp0289069   167.5   1520   7.24     NP_788867   AAO41635 C3G-PD   FBpp0289070   141.1   1285   6.41     NP_001138166   ACL82898 C3G-PE   FBpp0289071   154.6   1417   7.09     NP_001138167   ACL82899 C3G-PF   FBpp0289072   159.2   1468   6.91     NP_001138168   ACL82900 C3G-PG   FBpp0289073   136.5   1234   6.55     NP_001138169   ACL82901 C3G-PH   FBpp0289753   172.1   1571   7.09     NP_572350   AAF46200
Additional Polypeptide Data & Comments
Reported size (kDa)	1383 (aa); 151 (kD predicted) (Ishimaru et al., 1999)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Ras-like guanine nucleotide exchange factor, N-terminal (IPR000651) Guanine-nucleotide dissociation stimulator CDC25 (IPR001895) Ras guanine nucleotide exchange factor (IPR008937) Ras guanine nucleotide exchange factor, domain (IPR023578) Ras guanine-nucleotide exchange factor, conserved site (IPR019804)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA141186   AA141843   AA804088   AE014298 AAF46200   AE014298 AAO41635   AE014298 ACL82898   AE014298 ACL82899   AE014298 ACL82900   AE014298 ACL82901   AF053358 AAC35280   AI388824   AQ025539   AY113355 AAM29360   AY231847   BG633083   BI165924   BI169447   BP552341   BQ103065   BT010019 AAQ22488   BT025818   CO153477   CO153671   CO154375   CO155981   CO156496   CO156549   CO157000   CO179534   CO184879   CO186633   CO190001   CO262804   CO266571   CO267993   CO275177   CO278175   CO278581   CO286638   CO290872   CO291779   CO292781   CO296443   CO297709   CO300704   CO300924   CO301469   CO306337   CO324603   CO328269   CO335533   CZ470700   CZ471682   CZ481069   CZ481070   EC052694   EC052704   EC052708   EC053773   EC054170   EC055062   EC055729   EC058954   EC062480   EC070955   EC080407   EC081350   EC082024   EC084502   EC085707   EC194595   EC194866   EC217633   EC218316   EC221321   EC229891   EC235283   EC235460   EC237819   EC238095   EC240664   EC244010   EC247045   EC254197   EC260281   EC261238   EC261333   EC261755   EC261822   EC262815   EC262996   EC263098   EC267819   EL871242   EL871361   EL871474   EV578437   EV585072   GH943758   GH945671   GH953115   GH955522   GH959685   GH963406   GH965383   GH967557   GH971179   GH973904   GH982451
UniProtKB/Swiss-Prot	O77086
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 4 -- 17     (Ishimaru et al., 1999) embryonic stage 11   visceral mesoderm   (Ishimaru et al., 1999) embryonic stage 14   embryonic/larval hindgut   (Ishimaru et al., 1999) Comment:late embryonic stage 14 embryonic/larval foregut   (Ishimaru et al., 1999) Comment:late embryonic stage 14 organism | segmentally repeated   (Ishimaru et al., 1999) Comment:late stage 14
Additional Descriptive Data
	C3G transcript expression is detected starting at embryonic stage 4, with low levels throughout the embryo, and high levels at the anterior and posterior poles. A moderate level of expression is detected in all tissues throughout embryonic development, with higher levels of expression in some tissues. At stage 11, levels are high in the visceral mesoderm. At late stage 14, a segmentally repeated pattern of expression becomes apparent. Expression is also detected in the hindgut and foregut. (Ishimaru et al., 1999)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0259228    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0259228 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 12-18 embryonic stages, during late pupal stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0259228 expression level Developmental Stage   Expression Level embryo 00-02hr    1205 embryo 02-04hr    807 embryo 04-06hr    795 embryo 06-08hr    1210 embryo 08-10hr    1163 embryo 10-12hr    1093 embryo 12-14hr    1073 embryo 14-16hr    1581 embryo 16-18hr    1125 embryo 18-20hr    961 embryo 20-22hr    1060 embryo 22-24hr    1013 larva L1    992 larva L2    972 larva L3 12hr old    797 larva L3 puffstage 1-2    370 larva L3 puffstage 3-6    623 larva L3 puffstage 7-9    677 white prepupae new    632 white prepupae 12hr    703 white prepupae 24hr    930 pupae 2d postWPP    1411 pupae 3d postWPP    640 pupae 4d postWPP    563 adult male 01day    849 adult male 05day    945 adult male 30day    882 adult female 01day    1123 adult female 05day    973 adult female 30day    1027 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (1205) embryo 02-04hr  (807) embryo 04-06hr  (795) embryo 06-08hr  (1210) embryo 08-10hr  (1163) embryo 10-12hr  (1093) embryo 12-14hr  (1073) embryo 14-16hr  (1581) embryo 16-18hr  (1125) embryo 18-20hr  (961) embryo 20-22hr  (1060) embryo 22-24hr  (1013) larva L1  (992) larva L2  (972) larva L3 12hr old  (797) larva L3 puffstage 1-2  (370) larva L3 puffstage 3-6  (623) larva L3 puffstage 7-9  (677) white prepupae new  (632) white prepupae 12hr  (703) white prepupae 24hr  (930) pupae 2d postWPP  (1411) pupae 3d postWPP  (640) pupae 4d postWPP  (563) adult male 01day  (849) adult male 05day  (945) adult male 30day  (882) adult female 01day  (1123) adult female 05day  (973) adult female 30day  (1027) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1205 embryo 02-04hr    807 embryo 04-06hr    795 embryo 06-08hr    1210 embryo 08-10hr    1163 embryo 10-12hr    1093 embryo 12-14hr    1073 embryo 14-16hr  (1581) embryo 16-18hr    1125 embryo 18-20hr    961 embryo 20-22hr    1060 embryo 22-24hr    1013 larva L1    992 larva L2    972 larva L3 12hr old    797 larva L3 puffstage 1-2    370 larva L3 puffstage 3-6    623 larva L3 puffstage 7-9    677 white prepupae new    632 white prepupae 12hr    703 white prepupae 24hr    930 pupae 2d postWPP  1411 pupae 3d postWPP    640 pupae 4d postWPP    563 adult male 01day    849 adult male 05day    945 adult male 30day    882 adult female 01day    1123 adult female 05day    973 adult female 30day    1027 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1205 embryo 02-04hr    807 embryo 04-06hr    795 embryo 06-08hr    1210 embryo 08-10hr    1163 embryo 10-12hr    1093 embryo 12-14hr    1073 embryo 14-16hr    1581 embryo 16-18hr    1125 embryo 18-20hr    961 embryo 20-22hr    1060 embryo 22-24hr    1013 larva L1    992 larva L2    972 larva L3 12hr old    797 larva L3 puffstage 1-2    370 larva L3 puffstage 3-6    623 larva L3 puffstage 7-9    677 white prepupae new    632 white prepupae 12hr    703 white prepupae 24hr    930 pupae 2d postWPP    1411 pupae 3d postWPP    640 pupae 4d postWPP    563 adult male 01day    849 adult male 05day    945 adult male 30day    882 adult female 01day    1123 adult female 05day    973 adult female 30day    1027 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1205 embryo 02-04hr    807 embryo 04-06hr    795 embryo 06-08hr    1210 embryo 08-10hr    1163 embryo 10-12hr    1093 embryo 12-14hr    1073 embryo 14-16hr    1581 embryo 16-18hr    1125 embryo 18-20hr    961 embryo 20-22hr    1060 embryo 22-24hr    1013 larva L1    992 larva L2    972 larva L3 12hr old    797 larva L3 puffstage 1-2    370 larva L3 puffstage 3-6    623 larva L3 puffstage 7-9    677 white prepupae new    632 white prepupae 12hr    703 white prepupae 24hr    930 pupae 2d postWPP    1411 pupae 3d postWPP    640 pupae 4d postWPP    563 adult male 01day    849 adult male 05day    945 adult male 30day    882 adult female 01day    1123 adult female 05day    973 adult female 30day    1027 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0259228 expression level Developmental Stage   Expression Level embryo 00-02hr    1205 embryo 02-04hr    807 embryo 04-06hr    795 embryo 06-08hr    1210 embryo 08-10hr    1163 embryo 10-12hr    1093 embryo 12-14hr    1073 embryo 14-16hr    1581 embryo 16-18hr    1125 embryo 18-20hr    961 embryo 20-22hr    1060 embryo 22-24hr    1013 larva L1    992 larva L2    972 larva L3 12hr old    797 larva L3 puffstage 1-2    370 larva L3 puffstage 3-6    623 larva L3 puffstage 7-9    677 white prepupae new    632 white prepupae 12hr    703 white prepupae 24hr    930 pupae 2d postWPP    1411 pupae 3d postWPP    640 pupae 4d postWPP    563 adult male 01day    849 adult male 05day    945 adult male 30day    882 adult female 01day    1123 adult female 05day    973 adult female 30day    1027 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (1205) embryo 02-04hr  (807) embryo 04-06hr  (795) embryo 06-08hr  (1210) embryo 08-10hr  (1163) embryo 10-12hr  (1093) embryo 12-14hr  (1073) embryo 14-16hr  (1581) embryo 16-18hr  (1125) embryo 18-20hr  (961) embryo 20-22hr  (1060) embryo 22-24hr  (1013) larva L1  (992) larva L2  (972) larva L3 12hr old  (797) larva L3 puffstage 1-2  (370) larva L3 puffstage 3-6  (623) larva L3 puffstage 7-9  (677) white prepupae new  (632) white prepupae 12hr  (703) white prepupae 24hr  (930) pupae 2d postWPP  (1411) pupae 3d postWPP  (640) pupae 4d postWPP  (563) adult male 01day  (849) adult male 05day  (945) adult male 30day  (882) adult female 01day  (1123) adult female 05day  (973) adult female 30day  (1027) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1205 embryo 02-04hr    807 embryo 04-06hr    795 embryo 06-08hr    1210 embryo 08-10hr    1163 embryo 10-12hr    1093 embryo 12-14hr    1073 embryo 14-16hr  1581 embryo 16-18hr    1125 embryo 18-20hr    961 embryo 20-22hr    1060 embryo 22-24hr    1013 larva L1    992 larva L2    972 larva L3 12hr old    797 larva L3 puffstage 1-2    370 larva L3 puffstage 3-6    623 larva L3 puffstage 7-9    677 white prepupae new    632 white prepupae 12hr    703 white prepupae 24hr    930 pupae 2d postWPP  1411 pupae 3d postWPP    640 pupae 4d postWPP    563 adult male 01day    849 adult male 05day    945 adult male 30day    882 adult female 01day    1123 adult female 05day    973 adult female 30day    1027 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1205 embryo 02-04hr    807 embryo 04-06hr    795 embryo 06-08hr    1210 embryo 08-10hr    1163 embryo 10-12hr    1093 embryo 12-14hr    1073 embryo 14-16hr    1581 embryo 16-18hr    1125 embryo 18-20hr    961 embryo 20-22hr    1060 embryo 22-24hr    1013 larva L1    992 larva L2    972 larva L3 12hr old    797 larva L3 puffstage 1-2    370 larva L3 puffstage 3-6    623 larva L3 puffstage 7-9    677 white prepupae new    632 white prepupae 12hr    703 white prepupae 24hr    930 pupae 2d postWPP    1411 pupae 3d postWPP    640 pupae 4d postWPP    563 adult male 01day    849 adult male 05day    945 adult male 30day    882 adult female 01day    1123 adult female 05day    973 adult female 30day    1027 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1205 embryo 02-04hr    807 embryo 04-06hr    795 embryo 06-08hr    1210 embryo 08-10hr    1163 embryo 10-12hr    1093 embryo 12-14hr    1073 embryo 14-16hr    1581 embryo 16-18hr    1125 embryo 18-20hr    961 embryo 20-22hr    1060 embryo 22-24hr    1013 larva L1    992 larva L2    972 larva L3 12hr old    797 larva L3 puffstage 1-2    370 larva L3 puffstage 3-6    623 larva L3 puffstage 7-9    677 white prepupae new    632 white prepupae 12hr    703 white prepupae 24hr    930 pupae 2d postWPP    1411 pupae 3d postWPP    640 pupae 4d postWPP    563 adult male 01day    849 adult male 05day    945 adult male 30day    882 adult female 01day    1123 adult female 05day    973 adult female 30day    1027 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0259228    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0259228 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, larval/adult central nervous system, adult crop, adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, adult salivary gland, larval trachea. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Heatmap Tissue   Expression Level Larval Central Nervous System no informative data Larval Midgut no informative data Larval Hindgut no informative data Larval Malpighian Tubules no informative data Larval Fat Body no informative data Larval Salivary Gland     Larval Trachea no informative data Larval Carcass no informative data Adult Head no informative data Adult Eye no informative data Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland no informative data Adult Heart no informative data Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary no informative data Adult Testis no informative data Adult Male Accessory Gland no informative data Adult Carcass no informative data modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_09
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0259228 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG14436-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | embryonic stage | heat sensitive, with Scer\GAL4Mef2.PR C3GScer\UAS.T:Hsap\MYC,T:Ggal\Myr3 semi-lethal | larval stage C3GMS viable, with Scer\GAL4pnr-MD237 C3GGD10209 C3GGD15286 Other Phenotypes Allele eclosion defective C3GMS short lived C3GMS visible, with Scer\GAL4dpp.blk1 C3GScer\UAS.T:Hsap\MYC,T:Ggal\Myr3 C3GΔ.Scer\UAS.T:Hsap\MYC,T:Ggal\Myr3 visible, with Scer\GAL4GMR.PF C3GScer\UAS.T:Hsap\MYC,T:Ggal\Myr3 C3GΔ.Scer\UAS.T:Hsap\MYC,T:Ggal\Myr3 Phenotype manifest in Allele anterior crossvein, with Scer\GAL4dpp.blk1 C3GΔ.Scer\UAS.T:Hsap\MYC,T:Ggal\Myr3 embryonic/larval somatic muscle C3GMS embryonic/larval somatic muscle, with Scer\GAL4Mef2.PR C3GScer\UAS.T:Hsap\MYC,T:Ggal\Myr3 eye, with Scer\GAL4GMR.PF C3GΔ.Scer\UAS.T:Hsap\MYC,T:Ggal\Myr3 C3GScer\UAS.T:Hsap\MYC,T:Ggal\Myr3 eye photoreceptor cell, with Scer\GAL4GMR.PF C3GΔ.Scer\UAS.T:Hsap\MYC,T:Ggal\Myr3 C3GScer\UAS.T:Hsap\MYC,T:Ggal\Myr3 interommatidial bristle, with Scer\GAL4GMR.PF C3GΔ.Scer\UAS.T:Hsap\MYC,T:Ggal\Myr3 C3GScer\UAS.T:Hsap\MYC,T:Ggal\Myr3 lens, with Scer\GAL4GMR.PF C3GScer\UAS.T:Hsap\MYC,T:Ggal\Myr3 ommatidium, with Scer\GAL4GMR.PF C3GΔ.Scer\UAS.T:Hsap\MYC,T:Ggal\Myr3 C3GScer\UAS.T:Hsap\MYC,T:Ggal\Myr3 pigment cell, with Scer\GAL4GMR.PF C3GΔ.Scer\UAS.T:Hsap\MYC,T:Ggal\Myr3 C3GScer\UAS.T:Hsap\MYC,T:Ggal\Myr3 rhabdomere, with Scer\GAL4GMR.PF C3GScer\UAS.T:Hsap\MYC,T:Ggal\Myr3 ventral longitudinal muscle C3GMS ventral longitudinal muscle, with Scer\GAL4Mef2.PR C3GScer\UAS.T:Hsap\MYC,T:Ggal\Myr3 wing disc, with Scer\GAL4dpp.blk1 C3GScer\UAS.T:Hsap\MYC,T:Ggal\Myr3 wing vein L3, with Scer\GAL4dpp.blk1 C3GΔ.Scer\UAS.T:Hsap\MYC,T:Ggal\Myr3 C3GScer\UAS.T:Hsap\MYC,T:Ggal\Myr3
Classical Alleles ( 8 )
For All Classical Alleles Show
Allele of C3G Class Mutagen Stocks Known lesion C3Gc06451 piggyBac transposase 1 -- C3Gd00589 P-element activity 1 -- C3Ge03301 piggyBac transposase 1 -- C3GEP1613 P-element activity 1 -- C3GGS8156 1 -- C3G2.5 0 -- C3GMS FLPase 0 Yes C3Gx13 P-element activity 0 Yes
Alleles Carried on Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Allele of C3G Class Mutagen Stocks Known lesion C3GGD10209 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes C3GGD15286 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes C3GJF01589 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes C3GKK101834 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes C3GScer\UAS.T:Hsap\MYC,T:Ggal\Myr3 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes C3GScer\UAS.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes C3GΔ.Scer\UAS.T:Hsap\MYC,T:Ggal\Myr3 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Partially disrupted in	Df(1)2.5 (Gorfinkiel et al., 2005) Df(1)x13 (Glise et al., 2005)
Disrupted in	Df(1)pod1Δ225 (Rothenberg et al., 2004) Df(1)pod1Δ291 (Rothenberg et al., 2004)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD10209} NA P{KK101834} NA P{TRiP.JF01589} NA P{UAS-C3G.T:Myc.T:Myr3} NA P{UAS-C3G.T:Myc} NA P{UAS-C3G.Δ.T:Myc.T:Myr3} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}C3GEP1613 NA P{GSV3}C3GGS8156 NA P{XP}C3Gd00589 NA miscellaneous insertions PBac{PB}C3Gc06451 NA PBac{RB}C3Ge03301 NA
Gene Ontology: Function, Process & Cellular Component ( 7 unique terms )
Terms Based on Experimental Evidence ( 6 terms )
Molecular Function
CV term Evidence References
Ras guanyl-nucleotide exchange factor activity inferred from mutant phenotype (assigned by UniProtKB) (Ishimaru et al., 1999)
Biological Process
CV term Evidence References
muscle attachment inferred from mutant phenotype (Shirinian et al., 2010) Ras protein signal transduction inferred from mutant phenotype (assigned by UniProtKB) (Ishimaru et al., 1999) regulation of Rap GTPase activity inferred from direct assay (Shirinian et al., 2010) sarcomere organization inferred from mutant phenotype (Shirinian et al., 2010) somatic muscle development inferred from mutant phenotype (Shirinian et al., 2010)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 2 terms )
Molecular Function
CV term Evidence References
Ras guanyl-nucleotide exchange factor activity inferred from sequence or structural similarity with Saccharomyces CDC25 (FlyBase, 1992-)
Biological Process ( 0 terms)
Cellular Component
CV term Evidence References
intracellular inferred from electronic annotation with InterPro:IPR000651, InterPro:IPR001895, InterPro:IPR008937 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions C3G allele Gene References unspecified ksr (Ishimaru et al., 1999) Nsf2 (Laviolette et al., 2005) R (Ishimaru et al., 1999) Ras85D (Ishimaru et al., 1999) rl (Ishimaru et al., 1999)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 58103 DroID - A comprehensive database of gene and protein interactions. • FBgn0259228 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 31618
Orthologs
Genome-wide drosophilid orthologs	Dana\GF20837 Dere\GG17654 Dere\GG17655 Dgri\GH24527 Dmoj\GI21607 Dper\GL26876 Dpse\GA16131 Dsec\GM12549 Dsim\GD16177 Dvir\GJ16545 Dwil\GK18859 Dwil\GK25837 Dyak\C3G Dyak\GE16443
Curated drosophilid orthologs	Dpse\GA12983   Dpse\GA16131   Dyak\C3G
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL013817-PA Aedes aegypti (mosquito) AGAP000932-PA Anopheles gambiae (mosquito) XP_393346.2 Apis mellifera (honey bee) XP_001943497.1 Acyrthosiphon pisum (pea aphid) 24003 Batrachochytrium dendrobatidis (chytrid fungus) 131740 Branchiostoma floridae (lancelet) XP_001898486.1 Brugia malayi (roundworm nematode) ENSBTAP00000032592 Bos taurus (bovine) CBP33662 Caenorhabditis briggsae (nematode) CE21520 Caenorhabditis elegans (worm) ENSCAFP00000029478 Canis familiaris (dog) JA02967 Caenorhabditis japonica (nematode) CPIJ019106 Culex pipens (mosquito) ENSCPOP00000017295 Cavia porcellus (guinea pig) DDB0233552 Dictyostelium discoideum (amoeba) ENSECAP00000017059 Equus caballus (horse) ENSGACP00000021147 Gymnopilus aculeatus (mushroom) ENSGALP00000005881 Gallus gallus (chicken) ENSP00000338266 Homo sapiens (human) ISCW013763-PA Ixodes scapularis (deer tick) 28890 Monocosta brevicollis (choanoflagellate) ENSMODP00000029468 Monodelphis domestica (gray opossum) ENSMMUP00000040047 Macaca mulatta (rhesus monkey) ENSMUSP00000092703 Mus musculus (house mouse) 201713 Nematostella vectensis (starlet anemone) XP_001602841.1 Nasonia vitripennis (wasp) ENSOANP00000023285 Ornithorynchus anatinus (platypus) ENSORLP00000005250 Oryzias latipes (japanese killifish) PHUM002401-PA Pediculus humanus (human body louse) ENSPPYP00000022096 Pongo pygmaeus (orangutan) 79925 Phytophthora ramorum (sudden oak death pathogen) 138772 Phytophthora sojae (root rot pathogen) ENSPTRP00000036777 Pan troglodytes (chimpanzee) YLR310C Saccharomyces cerevisiae (budding yeast) Smp_144600 Schistosoma mansoni (trematode parasite) ENSTNIP00000010319 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000046905 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000003111 Xenopus tropicalis (frog) YALI0D22286g Yarrowia lipolytica (nonconventional yeast) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB13935 NV14565 LH21652 ISCW013763 FBgn0068524 FBgn0227796 FBgn0203729 FBgn0187809 FBgn0167489 JGI_V11_229485 FBgn0076147 FBgn0164457 FBgn0144337 FBgn0131983 FBgn0109877 FBgn0097843 CPIJ019106 ACYPI008701 GB18294 AGAP000932 AAEL013817 PHUM211010 GLEAN_04461
Stocks & Reagents
Stocks Listed in FlyBase ( 11 )
Bloomington	11245 w1118 P{EP}C3GEP1613 31116 y1 v1; P{TRiP.JF01589}attP2
Harvard	- P{XP}C3Gd00589 - PBac{PB}C3Gc06451 - PBac{RB}C3Ge03301
Kyoto	201430 y1 w67c23 P{GSV3}C3GGS8156/Binsinscy
VDRC	v105664 P{KK101834}VIE-260B v29828 w1118; P{GD15286}v29828 v29829 w1118; P{GD15286}v29829 v21306 w1118; P{GD10209}v21306/TM3 v21307 w1118; P{GD10209}v21307/CyO
Genomic Clones ( 1 )
	BACR48O24 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 94 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GM12790 IP03271 RE10624
Other clones	yak-em_C3G
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RE05925
Other clones	14194 30789 35793 54328 58684 66514 70399 72224 144392 149444 214249.10 214535.4 522451 522680 523634 527026 528042 533549 542460 558040 CK01.9A.F8 CK01.15A.G9 CK01.21C.D2 CK01.24A.F10 CK01.30C.H6 CK01.57D.C9 CK01.60B.G7 CK01.76B.D8 CK01.97B.B4 CK01.102D.D5 CK01.109D.F9 CK01601 CK02573 Dmel_dig_015846_0652_0815 Dmel_dig_018784_0711_2718 EC28727 EC052694 EC052704 EC052708 EC053773 EC054170 EC055062 EC055729 EK021655 EK045911 EK059648 EK063428 EK075429 EK086415 EK089902 EK116653 EK129201 EK130502 EK146919 EK170402 EK179219 EK179706 EK181126 EK194338 EK198150 EK200940 EK213648 EK250821 EN02042 EN02545 EN04017 EN07141 EN08202 EN08264 EN09730 EN10648 EP08846 FGM218C01 GH19850 IP03371 IP03471 IP03571 RP004096227 RP004135258 RP004220918 RP004227648 RP004247348 RP004373841 RP004416138 RP004465975 RP004492602 RP004514854 RP004553405 UUGC0461
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0259228 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 31618 31619
Antibody Information
	polyclonal (Shirinian et al., 2010)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: C3G CG14436 (FlyBase Genome Annotators, 2008)
Additional comments	Annotations CG3126 and CG14436 merged as CG42328 in release 5.9 of the genome annotation. (FlyBase Genome Annotators, 2008)
Other Comments
	dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) Overactivation of C3G during development results in cell fate changes and overproliferation, mimicking overactivity of the RAS-MAPK pathway. (Ishimaru et al., 1999)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_132122 NM_176694 NM_001144694 NM_001144695 NM_001144696 NM_001144697 RefSeq (Proteins) • NP_572350 NP_788867 NP_001138166 NP_001138167 NP_001138168 NP_001138169 DDBJ / EMBL / GenBank • AA804088 AAC35280 AAF46200 AAM29360 AAO41635 AAQ22488 ACL82898 ACL82899 ACL82900 ACL82901 AF053358 AQ025539 AY113355 BI169447 BT010019 CZ470700 CZ471682 CZ481069 CZ481070 Entrez Gene - A searchable database of RefSeq genes. • 31618 UniProtKB/Swiss-Prot • O77086
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Ras-like guanine nucleotide exchange factor, N-terminal (IPR000651) Guanine-nucleotide dissociation stimulator CDC25 (IPR001895) Ras guanine nucleotide exchange factor (IPR008937) Ras guanine nucleotide exchange factor, domain (IPR023578) Ras guanine-nucleotide exchange factor, conserved site (IPR019804)
Linkouts
	BioGRID - A database of protein and genetic interactions • 58103 DroID - A comprehensive database of gene and protein interactions. • FBgn0259228 DRSC - Results from RNAi screens. • FBgn0259228 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0259228 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG14436-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0259228 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 31618 31619 InParanoid A subset of ortholog calls from InParanoid. • AAEL013817-PA Aedes aegypti (mosquito) AGAP000932-PA Anopheles gambiae (mosquito) XP_393346.2 Apis mellifera (honey bee) XP_001943497.1 Acyrthosiphon pisum (pea aphid) 24003 Batrachochytrium dendrobatidis (chytrid fungus) 131740 Branchiostoma floridae (lancelet) XP_001898486.1 Brugia malayi (roundworm nematode) ENSBTAP00000032592 Bos taurus (bovine) CBP33662 Caenorhabditis briggsae (nematode) CE21520 Caenorhabditis elegans (worm) ENSCAFP00000029478 Canis familiaris (dog) JA02967 Caenorhabditis japonica (nematode) CPIJ019106 Culex pipens (mosquito) ENSCPOP00000017295 Cavia porcellus (guinea pig) DDB0233552 Dictyostelium discoideum (amoeba) ENSECAP00000017059 Equus caballus (horse) ENSGACP00000021147 Gymnopilus aculeatus (mushroom) ENSGALP00000005881 Gallus gallus (chicken) ENSP00000338266 Homo sapiens (human) ISCW013763-PA Ixodes scapularis (deer tick) 28890 Monocosta brevicollis (choanoflagellate) ENSMODP00000029468 Monodelphis domestica (gray opossum) ENSMMUP00000040047 Macaca mulatta (rhesus monkey) ENSMUSP00000092703 Mus musculus (house mouse) 201713 Nematostella vectensis (starlet anemone) XP_001602841.1 Nasonia vitripennis (wasp) ENSOANP00000023285 Ornithorynchus anatinus (platypus) ENSORLP00000005250 Oryzias latipes (japanese killifish) PHUM002401-PA Pediculus humanus (human body louse) ENSPPYP00000022096 Pongo pygmaeus (orangutan) 79925 Phytophthora ramorum (sudden oak death pathogen) 138772 Phytophthora sojae (root rot pathogen) ENSPTRP00000036777 Pan troglodytes (chimpanzee) YLR310C Saccharomyces cerevisiae (budding yeast) Smp_144600 Schistosoma mansoni (trematode parasite) ENSTNIP00000010319 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000046905 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000003111 Xenopus tropicalis (frog) YALI0D22286g Yarrowia lipolytica (nonconventional yeast) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 31618 modMine - Data generated by the modENCODE project. • FBgn0259228 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB13935 NV14565 LH21652 ISCW013763 FBgn0068524 FBgn0227796 FBgn0203729 FBgn0187809 FBgn0167489 JGI_V11_229485 FBgn0076147 FBgn0164457 FBgn0144337 FBgn0131983 FBgn0109877 FBgn0097843 CPIJ019106 ACYPI008701 GB18294 AGAP000932 AAEL013817 PHUM211010 GLEAN_04461
Synonyms & Secondary IDs ( 7 )
Reported As
Symbol Synonym	C3G (Gorfinkiel et al., 2005, Laviolette et al., 2005, Quinones-Coello, 2007, Bakal et al., 2007, Bakal et al., 2007, Raymond et al., 2004, Stofanko et al., 2008, Glise et al., 2005, Shirinian et al., 2010) C(3)G (Rothenberg et al., 2004, Patil and Kai, 2010) CG3126 (Laviolette et al., 2005, Quinones-Coello, 2007, Larschan et al., 2007, Larschan et al., 2007, Keleman et al., 2009.8.5) CG14436   CG42328 (FlyBase Genome Annotators, 2008, Ni et al., 2008) DC3G (Ishimaru et al., 1999, Ishimaru et al., 1999) DC3-G (Raymond et al., 2004)
Name Synonym	C3G (Shirinian et al., 2010) CG14436   CG42328 (FlyBase Genome Annotators, 2008)
Secondary FlyBase IDs
	FBgn0026145 FBgn0029902
References ( 31 )
Generate a list of	All references relating to this gene ( 31 )
List References by type	Research paper  ( 13 ) Supplementary material  ( 7 ) Review  ( 1 ) Personal communication to FlyBase  ( 3 ) Abstract  ( 1 ) FlyBase analysis  ( 2 ) Computer file  ( 3 ) Curated genome annotation  ( 1 )
Recent research papers ( 2 )
	Patil and Kai, 2010, Curr. Biol. 20(8): 724--730 Repression of Retroelements in Drosophila Germline via piRNA Pathway by the Tudor Domain Protein Tejas. [FBrf0211166] Shirinian et al., 2010, PLoS ONE 5(3): e9403 The Rap1 Guanine Nucleotide Exchange Factor C3G Is Required for Preservation of Larval Muscle Integrity in Drosophila melanogaster. [FBrf0210183] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...