FlyBase Gene Report: Dmel\C3G

General Information

Symbol

Dmel\C3G

Species

D. melanogaster

Name

C3G guanyl-nucleotide exchange factor

Annotation Symbol

CG42328

Feature Type

protein_coding_gene

FlyBase ID

FBgn0259228

Gene Model Status

Current

Stock Availability

14 publicly available

Gene Snapshot

C3G guanyl-nucleotide exchange factor is expressed in the developing embryonic muscles and plays a role in body wall muscle development during larval stages. [Date last reviewed: 2016-08-18]

Other Summaries

Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

c(3)G, DC3G

Key Links

Genomic Location

Cytogenetic map

6D2-6D2

Sequence location

X:6,780,790..6,803,593 [-]

Recombination map

1-18

Sequence

Get Decorated FASTA

Genomic Maps

GBrowse JBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

NCBI Genome Data Viewer

UCSC Genome Browser

Ensembl Genome Browser

PopFly Genome Browser

GO Summary Ribbons

[Detailed GO annotations]

Families, Domains and Molecular Function

Gene Group Membership (FlyBase)

RAS GUANINE NUCLEOTIDE EXCHANGE FACTORS

Protein Family (UniProt, Sequence Similarities)

Protein Domains/Motifs

InterPro

Ras-like guanine nucleotide exchange factor, N-terminal; Ras guanine-nucleotide exchange factors catalytic domain; Ras-like guanine nucleotide exchange factor; Ras guanine-nucleotide exchange factor, conserved site; Ras guanine nucleotide exchange factor domain superfamily; Ras guanine-nucleotide exchange factor catalytic domain superfamily

Molecular Function (see GO section for details)

Experimental Evidence

Rap guanyl-nucleotide exchange factor activity; Ras guanyl-nucleotide exchange factor activity

Predictions / Assertions

Summaries

Gene Group Membership

RAS GUANINE NUCLEOTIDE EXCHANGE FACTORS -: RasGEFs are Guanine Nucleotide Exchange Factors (GEFs) for Ras family GTPases. The RasGEFs are characterized by a CDC25 homology domain. (Adapted from FBrf0220530).

UniProt Contributed Function Data

Guanine nucleotide-releasing protein that binds to SH3 domain of Crk. Transduces signals from Crk to activate RAS. Also involved in MAPK activation.

(UniProt, O77086)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\C3G or the JBrowse view of Dmel\C3G for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.50

Gene model reviewed during 5.47

Gene model reviewed during 5.55

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

C3G-RC

FBtr0299791

NM_176694

7553

1520

C3G-RD

FBtr0299792

NM_001144694

5397

1285

C3G-RE

FBtr0299793

NM_001144695

5651

1417

C3G-RF

FBtr0299794

NM_001144696

5804

1468

C3G-RG

FBtr0299795

NM_001144697

5244

1234

C3G-RH

FBtr0300526

NM_132122

6467

1571

C3G-RI

FBtr0334967

NM_001272362

5648

1414

C3G-RJ

FBtr0334968

NM_001272363

5027

1234

C3G-RK

FBtr0334969

NM_001272364

5804

1468

C3G-RL

FBtr0340087

NM_001298037

6103

1357

Additional Transcript Data and Comments

Reported size (kB)

7.0 (northern blot)

(Ishimaru et al., 1999)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

C3G-PC

FBpp0289069

167.5

1520

7.24

NP_788867

AAO41635

C3G-PD

FBpp0289070

141.1

1285

6.41

NP_001138166

ACL82898

C3G-PE

FBpp0289071

154.6

1417

7.09

NP_001138167

ACL82899

C3G-PF

FBpp0289072

159.2

1468

6.91

NP_001138168

ACL82900

C3G-PG

FBpp0289073

136.5

1234

6.55

NP_001138169

ACL82901

C3G-PH

FBpp0289753

172.1

1571

7.09

NP_572350

AAF46200

C3G-PI

FBpp0306980

153.7

1414

6.78

NP_001259291

AGB95136

C3G-PJ

FBpp0306981

136.5

1234

6.55

NP_001259292

AGB95137

C3G-PK

FBpp0306982

160.7

1468

7.57

NP_001259293

AGB95138

C3G-PL

FBpp0309086

148.5

1357

6.89

NP_001284966

AHN59437

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1234 aa isoforms: C3G-PG, C3G-PJ

Additional Polypeptide Data and Comments

Reported size (kDa)

1383 (aa); 151 (kD predicted)

(Ishimaru et al., 1999)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\C3G using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Gene Ontology (9 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

Rap guanyl-nucleotide exchange factor activity

inferred from direct assay

(Shirinian et al., 2010)

Ras guanyl-nucleotide exchange factor activity

inferred from mutant phenotype

(assigned by UniProt )

(Ishimaru et al., 1999)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (6 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

muscle attachment

inferred from mutant phenotype

(Shirinian et al., 2010)

positive regulation of GTPase activity

inferred from direct assay

(Shirinian et al., 2010)

Ras protein signal transduction

inferred from mutant phenotype

(assigned by UniProt )

(Ishimaru et al., 1999)

regulation of GTPase activity

inferred from direct assay

(Shirinian et al., 2010)

sarcomere organization

inferred from mutant phenotype

(Shirinian et al., 2010)

somatic muscle development

inferred from mutant phenotype

(Shirinian et al., 2010)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

intracellular

inferred from electronic annotation with InterPro:IPR008937

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism | ubiquitous

(Shirinian et al., 2010)

visceral mesoderm

(Shirinian et al., 2010)

somatic mesoderm

(Shirinian et al., 2010)

embryonic/larval central nervous system

(Shirinian et al., 2010)

embryonic/larval somatic muscle

(Shirinian et al., 2010)

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 17

(Ishimaru et al., 1999)

embryonic stage 11

visceral mesoderm

(Ishimaru et al., 1999)

embryonic stage 14

embryonic/larval hindgut

Comment: late embryonic stage 14

(Ishimaru et al., 1999)

embryonic/larval foregut

Comment: late embryonic stage 14

(Ishimaru et al., 1999)

organism | segmentally repeated

Comment: late stage 14

(Ishimaru et al., 1999)

Additional Descriptive Data

C3G is expressed ubiquitously in embryos with stronger expression in the founder cells of the developing visceral mesoderm. It is also observed in the visceral mesoderm at later stages, in somatic muscle and in the CNS.

(Shirinian et al., 2010)

C3G transcript expression is detected starting at embryonic stage 4, with low levels throughout the embryo, and high levels at the anterior and posterior poles. A moderate level of expression is detected in all tissues throughout embryonic development, with higher levels of expression in some tissues. At stage 11, levels are high in the visceral mesoderm. At late stage 14, a segmentally repeated pattern of expression becomes apparent. Expression is also detected in the hindgut and foregut.

(Ishimaru et al., 1999)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\C3G in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\C3G

View exonic expression by tissue for Dmel\C3G

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0259228

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0259228

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG14436-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3126

Flygut - An atlas of the Drosophila adult midgut

FBgn0259228

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 10 )

For All Classical and Insertion Alleles Show

Allele of C3G

Class

Mutagen

Associated Insertion

Stocks

Known lesion

C3G^EP1613

P-element activity

P{EP}C3G^EP1613

Yes

C3G^c06451

piggyBac activity

PBac{PB}C3G^c06451

Yes

C3G^d00589

P-element activity

P{XP}C3G^d00589

Yes

C3G^e03301

piggyBac activity

Yes

Yes

Yes

C3G^{MI06062-GFSTF.1}

phiC31 integrase

Mi{PT-GFSTF.1}C3G^{MI06062-GFSTF.1}

Yes

C3G^2.5

C3G^MS

FLPase

Yes

C3G^x13

P-element activity

Yes

Other relevant insertions

Transgenic Constructs ( 9 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of C3G

Allele of C3G

Mutagen

Associated Transgenic Construct

Stocks

C3G^{UAS.Tag:MYC,Tag:M(c-SRC)}

P{UAS-C3G.T:Myc.T:Myr3}

C3G^{Δ.UAS.Tag:MYC,Tag:M(c-SRC)}

P{UAS-C3G.Δ.T:Myc.T:Myr3}

Transgenic constructs containing regulatory region of C3G

Deletions and Duplications ( 4 )

Partially disrupted in

Df(1)2.5

(Gorfinkiel et al., 2005)

Df(1)x13

(Glise et al., 2005)

Disrupted in

Df(1)pod1^Δ225

(Rothenberg et al., 2003)

Df(1)pod1^Δ291

(Rothenberg et al., 2003)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die before end of embryonic stage | heat sensitive, with Scer\GAL4^Mef2.PR

C3G^{UAS.Tag:MYC,Tag:M(c-SRC)}

partially lethal - majority die

C3G^MS

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

C3G^GD10209

viable, with Scer\GAL4^Mef2.PR

viable, with Scer\GAL4^pnr-MD237

viable, with Scer\GAL4^tin.CΔ4

C3G^GD10209

Other Phenotypes

Allele

eclosion defective

C3G^MS

increased cell number | adult stage, with Scer\GAL4^GMR.PF

C3G^{UAS.Tag:MYC,Tag:M(c-SRC)}

short lived

C3G^MS

size defective | adult stage, with Scer\GAL4^GMR.PF

C3G^{UAS.Tag:MYC,Tag:M(c-SRC)}

some die during embryonic stage | heat sensitive, with Scer\GAL4^Mef2.PR

C3G^{UAS.Tag:MYC,Tag:M(c-SRC)}

some die during larval stage

C3G^MS

visible, with Scer\GAL4^dpp.blk1

visible, with Scer\GAL4^GMR.PF

visible, with Scer\GAL4^GMR.PU

C3G^EP1613

Phenotype manifest in

Allele

anterior crossvein, with Scer\GAL4^dpp.blk1

C3G^{Δ.UAS.Tag:MYC,Tag:M(c-SRC)}

embryonic/larval hemocyte | third instar larval stage, with Scer\GAL4^Pxn.PS

C3G^EP1613

embryonic/larval somatic muscle

C3G^MS

embryonic/larval somatic muscle, with Scer\GAL4^Mef2.PR

C3G^{UAS.Tag:MYC,Tag:M(c-SRC)}

eye, with Scer\GAL4^GMR.PF

eye, with Scer\GAL4^GMR.PU

C3G^EP1613

eye photoreceptor cell, with Scer\GAL4^GMR.PF

interommatidial bristle, with Scer\GAL4^GMR.PF

lens, with Scer\GAL4^GMR.PF

C3G^{UAS.Tag:MYC,Tag:M(c-SRC)}

ommatidium, with Scer\GAL4^GMR.PF

pigment cell, with Scer\GAL4^GMR.PF

rhabdomere, with Scer\GAL4^GMR.PF

C3G^{UAS.Tag:MYC,Tag:M(c-SRC)}

ventral longitudinal muscle

C3G^MS

ventral longitudinal muscle, with Scer\GAL4^Mef2.PR

C3G^{UAS.Tag:MYC,Tag:M(c-SRC)}

wing disc, with Scer\GAL4^dpp.blk1

C3G^{UAS.Tag:MYC,Tag:M(c-SRC)}

wing vein L3, with Scer\GAL4^dpp.blk1

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (6)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\RAPGEF1

12 of 15

Yes

Hsap\RAPGEF2

1 of 15

Hsap\RASGRF1

1 of 15

Yes

Hsap\RASGRF2

1 of 15

Yes

Hsap\SOS1

1 of 15

Hsap\SOS2

1 of 15

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (7)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Rapgef1

10 of 15

Yes

Mmus\Rapgef2

1 of 15

Mmus\Rapgef6

1 of 15

Mmus\Rasgrf1

1 of 15

Yes

Mmus\Rasgrf2

1 of 15

Yes

Mmus\Sos1

1 of 15

Mmus\Sos2

1 of 15

Rattus norvegicus (Norway rat) (1)

Rnor\Rapgef1

6 of 13

Yes

Xenopus tropicalis (Western clawed frog) (1)

Xtro\rapgef1

4 of 12

Yes

Danio rerio (Zebrafish) (5)

Drer\rapgef1b

11 of 15

Yes

Drer\rapgef1a

7 of 15

Yes

Drer\rasgrf2b

1 of 15

Yes

Drer\si:ch211-234p18.3

1 of 15

Yes

Drer\sos1

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (4)

Cele\Y34B4A.4

9 of 15

Yes

Cele\pxf-1

1 of 15

Cele\sos-1

1 of 15

Cele\Y38C1AB.7

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (5)

Atha\AOP1

1 of 9

Yes

Atha\AOP3

1 of 9

Yes

Atha\AT1G15540

1 of 9

Yes

Atha\AT1G80320

1 of 9

Yes

Atha\AT5G51310

1 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\CDC25

4 of 15

Yes

Scer\LTE1

1 of 15

Schizosaccharomyces pombe (Fission yeast) (2)

Spom\ste6

3 of 12

Yes

Spom\efc25

1 of 12

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091901F5 )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\C3G

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA16131

Drosophila persimilis

Dper\GL26876

Drosophila willistoni

Dwil\GK25837

Drosophila virilis

Dvir\GJ16545

Drosophila mojavensis

Dmoj\GI21607

Drosophila grimshawi

Dgri\GH24527

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091500N8 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17006979

Musca domestica

House fly

Mdoa\17005400

Musca domestica

House fly

Mdoa\17015900

Glossina morsitans

Tsetse fly

Gmor\GMOY007326

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_14481

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL013817

Anopheles darlingi

American malaria mosquito

Adar\ADAC003939

Anopheles gambiae

Malaria mosquito

Agam\AGAP000932

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ019106

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W02GC )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Danaus plexippus

Monarch butterfly

Dple\DPOGS204405-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL015678

Apis florea

Little honeybee

Aflo\2904620948

Apis mellifera

Western honey bee

Amel\GB49150

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP25894

Bombus terrestris

Buff-tailed bumblebee

Bter\15201617

Bombus terrestris

Buff-tailed bumblebee

Bter\15201623

Bombus terrestris

Buff-tailed bumblebee

Bter\15214328

Linepithema humile

Argentine ant

Lhum\LH21652

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:002867

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG009786

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_01601

Tribolium castaneum

Red flour beetle

Tcas\TC004461

Pediculus humanus

Human body louse

Phum\PHUM211010

Rhodnius prolixus

Kissing bug

Rpro\RPRC008080

Cimex lectularius

Bed bug

Clec\CLEC007753

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI008701

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_08389

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X02DW )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR009676

Ixodes scapularis

Black-legged tick

Isca\ISCW013763

Stegodyphus mimosarum

African social velvet spider

Smim\KFM63473.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM63474.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur02g01450

Tetranychus urticae

Two-spotted spider mite

Turt\tetur02g01430

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_229485

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G03MV )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Rapgef1

Ciona intestinalis

Vase tunicate

Cint\LOC100177942

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000001832

Gallus gallus

Domestic chicken

Ggal\RAPGEF1

Human Disease Model Data

FlyBase Human Disease Model Reports

Alleles Reported to Model Human Disease (Disease Ontology)

Download

Models ( 0 )

Allele

Disease

Evidence

References

Interactions ( 0 )

Allele

Disease

Interaction

References

Comments ( 0 )

Human Orthologs (via DIOPT v7.1)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

Complementation?

Transgene?

RAPGEF1; Rap guanine nucleotide exchange factor 1

12 of 15

600303

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please look at the Interaction Group reports for full details of the physical interactions

protein-protein

Interacting group

Assay

References

C3G - drk

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

C3G

suppressible

ksr

(Ishimaru et al., 1999)

C3G

suppressible

Rap1

(Ishimaru et al., 1999)

C3G

suppressible

Ras85D

(Ishimaru et al., 1999)

C3G

suppressible

(Ishimaru et al., 1999)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Cdc42

enhanceable

C3G

(Raymond et al., 2004)

Nsf2

suppressible

C3G

(Laviolette et al., 2005)

Rac1

enhanceable

C3G

(Raymond et al., 2004)

RacGAP84C

enhanceable

C3G

(Raymond et al., 2004)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

58103

DroID - A comprehensive database of gene and protein interactions.

FBgn0259228

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

31618

Pathways

Gene Group - Pathway Membership (FlyBase)

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-18

Cytogenetic map

6D2-6D2

Sequence location

X:6,780,790..6,803,593 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

6D2-6D2

Limits computationally determined from genome sequence between P{EP}C3G^EP1613 and P{EP}EP1388^EP1388&P{EP}EP1573

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

6C7-6D2

(Ishimaru et al., 1999)

Experimentally Determined Recombination Data

Location

1-18

(FlyBase, 2016)

1-18.9

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (14)

Bloomington

11245

w¹¹¹⁸ P{EP}C3G^EP1613

31116

y¹ v¹; P{TRiP.JF01589}attP2

41508

y¹ w^* Mi{MIC}C3G^MI06062

80404

y¹ sc^* v¹; P{TRiP.HMC06640}attP40

d00589

c06451

e03301

v105664

v29828

w¹¹¹⁸; P{GD15286}v29828

v29829

w¹¹¹⁸; P{GD15286}v29829

v21306

w¹¹¹⁸; P{GD10209}v21306/TM3

v21307

w¹¹¹⁸; P{GD10209}v21307/CyO

More stocks available...

Genomic Clones (14)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (86)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0259228

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RE05925

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0259228

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

31618

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Shirinian et al., 2010)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: C3G CG14436

(FlyBase Genome Annotators, 2008)

Additional comments

Annotations CG3126 and CG14436 merged as CG42328 in release 5.9 of the genome annotation.

(FlyBase Genome Annotators, 2008)

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Overactivation of C3G during development results in cell fate changes and overproliferation, mimicking overactivity of the RAS-MAPK pathway.

(Ishimaru et al., 1999)

Origin and Etymology

Discoverer

Etymology

Named "C3G" after the human ortholog.

(FlyBase, 2009-)

Identification

External Crossreferences and Linkouts ( 82 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

31618

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

O77086

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0259228

InterPro - A database of protein families, domains and functional sites

Linkouts

BioGRID - A database of protein and genetic interactions.

58103

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center cDNA clones

FBgn0259228

DroID - A comprehensive database of gene and protein interactions.

FBgn0259228

DRSC - Results frm RNAi screens

FBgn0259228

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0259228

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG14436-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0259228

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3126

Flygut - An atlas of the Drosophila adult midgut

FBgn0259228

FlyMine - An integrated database for Drosophila genomics

FBgn0259228

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

31618

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC004461

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

31618

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG42328

MIST (protein-protein) - An integrated Molecular Interaction Database

modMine - A data warehouse for the modENCODE project

FBgn0259228

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

C(3)G

(Rog et al., 2017, Rothenberg et al., 2003)

C3G

(Sawant et al., 2018, Gene Disruption Project members, 2015-, Sen et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Diez et al., 2011, Friedman et al., 2011, Shirinian et al., 2010, van Dam et al., 2009, Stofanko et al., 2008, Bakal et al., 2007, Bakal et al., 2007, Quinones-Coello, 2007, Dupuy et al., 2005, Glise et al., 2005, Gorfinkiel et al., 2005, Laviolette et al., 2005, Raymond et al., 2004)

CG14436

CG3126

(Christis and Munro, 2012, Keleman et al., 2009.8.5, van Dam et al., 2009, Larschan et al., 2007, Larschan et al., 2007, Quinones-Coello, 2007, Laviolette et al., 2005)

CG42328

(Perkins et al., 2009, FlyBase Genome Annotators, 2008, Ni et al., 2008)

DC3-G

(Raymond et al., 2004)

DC3G

(Ishimaru et al., 1999, Ishimaru et al., 1999)

c(3)G

(Hughes et al., 2018, Rothenberg et al., 2003)

Name Synonyms

C3G

(Shirinian et al., 2010)

C3G guanyl-nucleotide exchange factor

C3G ortholog

CG42328

(FlyBase Genome Annotators, 2008)

Secondary FlyBase IDs

FBgn0026145
FBgn0029902

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (56)

The following external sites may use different assemblies or annotations than FlyBase.

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