FlyBase Gene Report: Dmel\Prim2

General Information

Symbol

Dmel\Prim2

Species

D. melanogaster

Name

DNA primase subunit 2

Annotation Symbol

CG5553

Feature Type

protein_coding_gene

FlyBase ID

FBgn0259676

Gene Model Status

Current

Stock Availability

7 publicly available

Gene Summary

DNA primase subunit 2 (Prim2) encodes a regulatory subunit of the DNA polymerase alpha-primase complex, which synthesizes short RNA-DNA primers on the lagging strand during DNA synthesis. [Date last reviewed: 2021-01-21] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

DNAprim, l(3)j10B2, DNA primase, DNApol-alpha60, DNA polymerase α 60kD

Key Links

Genomic Location

Cytogenetic map

77B6-77B6

Sequence location

3L:20,359,833..20,361,585 [-]

Recombination map

3-47

RefSeq locus

NT_037436 REGION:20359833..20361585

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (7 terms)

Molecular Function (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (6 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

involved_in compound eye morphogenesis

inferred from mutant phenotype

(Chen et al., 2000)

involved_in DNA replication, synthesis of primer

inferred from direct assay

(Kuroda et al., 1990, Cotterill et al., 1987, Kaguni et al., 1983)

involved_in DNA replication

inferred from mutant phenotype

(Chen et al., 2000)

involved_in DNA-templated DNA replication

inferred from direct assay

(Kuroda et al., 1990, Kaguni et al., 1983, Villani et al., 1980)

involved_in mitotic cell cycle

inferred from mutant phenotype

(Chen et al., 2000)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in DNA replication initiation

inferred from biological aspect of ancestor with PANTHER:PTN000830013

(GO Reference Genome Project, 2011-)

involved_in DNA replication, synthesis of primer

inferred from biological aspect of ancestor with PANTHER:PTN000830013

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR007238

(InterPro Project Members, 2004-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

part_of alpha DNA polymerase:primase complex

inferred from direct assay

(Kuroda et al., 1990, Cotterill et al., 1987, Kaguni et al., 1983, Kaguni et al., 1983, Villani et al., 1980)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

part_of alpha DNA polymerase:primase complex

inferred from biological aspect of ancestor with PANTHER:PTN000056837

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

DNA POLYMERASE ALPHA-PRIMASE

Protein Family (UniProt)

Belongs to the eukaryotic-type primase large subunit family. (Q9VPH2)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

DNA POLYMERASE ALPHA-PRIMASE -: DNA polymerase alpha-primase synthesizes short RNA-DNA primers on the lagging strand during DNA synthesis. It is a heterotetramer composed of two polymerase subunits (DNApol-α180 and DNApol-α73) and two primase subunits (DNApol-α50 and DNApol-α60). DNApol-α180 and DNApol-α50 encode the DNA polymerase and RNA primase catalytic activities, respectively, while DNApol-α73 and DNApol-α60 encode the regulatory subunits. (Adapted from PMID:30005324.)

Protein Function (UniProtKB)

Regulatory subunit of the DNA primase complex and component of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex) which play an essential role in the initiation of DNA synthesis (PubMed:6403945, PubMed:6409898, PubMed:6773966). During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit PolA1, an accessory subunit PolA2 and two primase subunits, the catalytic subunit Prim1 and the regulatory subunit Prim2) is recruited to DNA at the replicative forks (By similarity). The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands (PubMed:6812052). These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively (By similarity). In the primase complex, both subunits are necessary for the initial di-nucleotide formation, but the extension of the primer depends only on the catalytic subunit (PubMed:6812052). Stabilizes and modulates the activity of the catalytic subunit (By similarity).

(UniProt, Q9VPH2)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Prim2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VPH2)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.39

Gene model reviewed during 5.45

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Prim2-RA

FBtr0078241

NM_143744

1753

533

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Prim2-PA

FBpp0077899

61.4

533

6.95

Q9VPH2

NP_652001

AAF51580

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Heterodimer of a catalytic subunit Prim1 and a regulatory subunit Prim2, also known as the DNA primase complex (PubMed:6403945, PubMed:6409898). Component of the alpha DNA polymerase complex (also known as the alpha DNA polymerase-primase complex) consisting of four subunits: the catalytic subunit PolA1, the regulatory subunit PolA2, and the primase complex subunits Prim1 and Prim2 respectively (PubMed:6403945, PubMed:6409898, PubMed:6773966). PolA1 associates with the DNA primase complex before association with PolA2 (PubMed:6409898).

(UniProt, Q9VPH2)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9VPH2

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Prim2 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

DNApol-alpha60_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.03

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of alpha DNA polymerase:primase complex

inferred from direct assay

(Kuroda et al., 1990, Cotterill et al., 1987, Kaguni et al., 1983, Kaguni et al., 1983, Villani et al., 1980)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Prim2 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Prim2

View exonic expression by tissue for Dmel\Prim2

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

44915/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0259676

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0259676

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5553

Flygut - An atlas of the Drosophila adult midgut

FBgn0259676

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 13 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Prim2

Transgenic constructs containing regulatory region of Prim2

Aberrations (Deficiencies and Duplications) ( 7 )

Inferred from experimentation ( 7 )

Gene disrupted in

Df(3L)BSC447

(Christensen et al., 2008.4.15)

Df(3L)BSC839

(Christensen et al., 2009.7.22)

Df(3L)Exel6136

(Ryder, 2004.4.26)

Df(3L)rdgC-co2

(Chen et al., 2000, Lukinova et al., 1999, Spradling et al., 1999, BDGP Project Members, 1994-1999)

In(3L)ppl¹

(Chen et al., 2000, Lukinova et al., 1999)

Gene not disrupted in

Df(3L)in61

(BDGP Project Members, 1994-1999)

Df(3L)ri-79c

(Chen et al., 2000, Lukinova et al., 1999)

Inferred from location ( 8 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal (with Prim2^j10B2Δ11)

Prim2^Sfaf240

lethal (with Prim2^j10B2Δ12)

Prim2^Sfaf240

lethal (with Prim2^ppl-1)

Prim2^Sfaf240

lethal (with Prim2^Sfaf240)

lethal | recessive

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

Prim2^GD4567

lethal - all die before end of pupal stage (with Prim2^Sfaf240)

Prim2^T2

lethal - all die before end of pupal stage (with Prim2^T2)

Prim2^Sfaf240

lethal - all die before end of pupal stage | recessive

Prim2^T2

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

Prim2^GD4567

partially lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Prim2^GD4567

viable

viable, with Scer\GAL4^tin.CΔ4

Prim2^GD4567

viable (with Prim2^j10B2Δ13)

Prim2^Sfaf240

viable (with Prim2^j10B2Δ14)

Prim2^Sfaf240

viable (with Prim2^j10B2Δ15)

Prim2^Sfaf240

viable (with Prim2^Sfaf240)

Other Phenotypes

Allele

increased cell death | third instar larval stage (with Prim2^Sfaf240)

Prim2^T2

increased cell death | third instar larval stage (with Prim2^T2)

Prim2^Sfaf240

increased occurrence of cell division | third instar larval stage (with Prim2^Sfaf240)

Prim2^T2

increased occurrence of cell division | third instar larval stage (with Prim2^T2)

Prim2^Sfaf240

some die during pupal stage, with Scer\GAL4^pnr-MD237

Prim2^GD4567

some die during pupal stage (with Prim2^Sfaf240)

Prim2^T2

some die during pupal stage (with Prim2^T2)

Prim2^Sfaf240

some die during pupal stage | recessive

Prim2^T2

visible, with Scer\GAL4^ey.PU

Prim2^NIG.5553R

visible (with Prim2^j10B2Δ13)

Prim2^Sfaf240

visible (with Prim2^j10B2Δ14)

Prim2^Sfaf240

visible (with Prim2^j10B2Δ15)

Prim2^Sfaf240

visible (with Prim2^Sfaf240)

visible | recessive

Phenotype manifest in

Allele

eye, with Scer\GAL4^ey.PU

Prim2^NIG.5553R

eye | anterior (with Prim2^Sfaf240)

Prim2^T2

eye | anterior (with Prim2^T2)

Prim2^Sfaf240

eye disc (with Prim2^Sfaf240)

Prim2^T2

eye disc (with Prim2^T2)

Prim2^Sfaf240

mitotic cell cycle (with Prim2^Sfaf240)

Prim2^T2

mitotic cell cycle (with Prim2^T2)

Prim2^Sfaf240

ommatidium

ommatidium (with Prim2^j10B2Δ13)

Prim2^Sfaf240

ommatidium (with Prim2^j10B2Δ14)

Prim2^Sfaf240

ommatidium (with Prim2^j10B2Δ15)

Prim2^Sfaf240

ommatidium (with Prim2^Sfaf240)

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (2)

Hsap\PRIM2

14 of 14

Yes

Hsap\LOC101930420

1 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (1)

Rnor\Prim2

14 of 14

Yes

Mus musculus (laboratory mouse) (1)

Mmus\Prim2

13 of 14

Yes

Xenopus tropicalis (Western clawed frog) (2)

Xtro\prim2

11 of 13

Yes

Xtro\smim14

1 of 13

Danio rerio (Zebrafish) (1)

Drer\prim2

14 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\pri-2

13 of 14

Yes

Anopheles gambiae (African malaria mosquito) (1)

Agam\AgaP_AGAP006387

12 of 12

Yes

Arabidopsis thaliana (thale-cress) (1)

Atha\EMB2813

13 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\PRI2

13 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\spp2

12 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Prim2. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (0)

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PRIM2; DNA primase subunit 2

14 of 14

176636

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

Off

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Prim2 - PolA1

ion exchange chromatography, molecular weight estimation by staining

(Kaguni et al., 1983)

Prim2 - PolA2

ion exchange chromatography, molecular weight estimation by staining

(Kaguni et al., 1983)

Prim2 - Prim1

ion exchange chromatography, molecular weight estimation by staining

(Kaguni et al., 1983)

Summary of Genetic Interactions

esyN Network Diagram

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

esyN Network Key:

Suppression

Enhancement

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

faf

suppressible

Prim2

(Chen et al., 2000)

External Data

Subunit Structure (UniProtKB)

(UniProt, Q9VPH2 )

Linkouts

BioGRID - A database of protein and genetic interactions.

69380

DroID - A comprehensive database of gene and protein interactions.

FBgn0259676

MIST (protein-protein) - An integrated Molecular Interaction Database

44915

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

DNA replication

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-47

Cytogenetic map

77B6-77B6

Sequence location

3L:20,359,833..20,361,585 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

77B6-77B6

Limits computationally determined from genome sequence between P{PZ}eRF1⁰⁰¹⁰³ and P{EP}trbl^EP3519

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Mapped by recombination.

(Chen et al., 2000)

77B6-77B7

(determined by in situ hybridisation)

(Chen et al., 2000, Spradling et al., 1999, BDGP Project Members, 1994-1999)

77B6-77B7

(Chen, 2000.8.1)

77B-77B

(determined by in situ hybridisation)

(Huikeshoven and Cotterill, 1999)

Experimentally Determined Recombination Data

Location

3-47

(FlyBase, 2016)

3-43.6

(Comeron et al., 2012)

Left of (cM)

(Chen et al., 2000)

Right of (cM)

Diap1

(Chen et al., 2000)

Notes

Stocks and Reagents

Stocks (7)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

5469

Prim2^L5/TM6C, Sb¹

603507

y¹ w¹; P{TKO-UAS.GS06224}attP40/CyO

12108

y¹ w^*; P{lacW}Prim2^j10B2/TM3, Sb¹

44584

y¹ sc^* v¹ sev²¹; P{TRiP.HMS02881}attP2/TM3, Sb¹

97354

y¹ w^*; TI{KozakGAL4}Prim2^{CR70541-KO-kG4}/TM3, Sb¹ Ser¹

NIG-Fly

5553R-1

P{NIG.5553R}1

VDRC

v30623

w¹¹¹⁸; P{GD4567}v30623

Genomic Clones (14)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (17)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GM13640

Other clones

MIP29196

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0259676

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0259676

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Source for merge of: DNAprim BcDNA:GM13640

(Misra, 2000.8.9)

Source for merge of: DNApol-α60 DNAprim

(FlyBase, 1996-)

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Prim2 DNApol-α60

(Marygold et al., 2020)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (25)

Reported As

Symbol Synonym

BcDNA:GM13640

CG5553

(Çiçek et al., 2016, Suyari et al., 2012, Ni et al., 2010.12.1)

DNA polα

(Frolov et al., 2003)

DNApol-alpha60

(Guichard et al., 2023)

DNApol-α60

DNAprim

(Lee et al., 2010, Mudiganti et al., 2010, Ayroles et al., 2009, Christensen et al., 2009.7.22, Christensen et al., 2008.4.15, Somma et al., 2008, Stanyon et al., 2004)

DNAprimase

(Cayirlioglu et al., 2003)

Dmpol α

(Takeuchi et al., 2004)

GM13640

(De Lucia et al., 2005)

PRI2

(Liu et al., 2018)

Pol

(Machida et al., 2005)

Polα 60 kDa

(Suyari et al., 2012)

Prim2

(Yamaguchi and Cotterill, 2023, Marygold et al., 2020, Gene Disruption Project members, 2018-, Transgenic RNAi Project members, 2017-)

S(faf)240

(Chen et al., 2000)

l(3)77ABe

(Lukinova et al., 1999)

l(3)j10B2

(Beaton, 2000.1.31, Meister and Braun, 1995.10, BDGP Project Members, 1994-1999)

pol α

(Mizushina et al., 2003)

polα

(Elenbaas et al., 2015)

Name Synonyms

DNA polymerase alpha 60kD subunit

DNA polymerase α 58,000 β subunit

(Villani et al., 1980)

DNA polymerase α 60,000 β subunit

(Kaguni et al., 1983)

DNA polymerase α 60kD

(Huikeshoven and Cotterill, 1999)

DNA primase

(Acharya et al., 2010, Somma et al., 2008, Brumby et al., 2004)

DNA primase subunit 2

(Marygold et al., 2020)

DNA replication primer

(Cho et al., 2005)

DNApol-alpha60

(Williams et al., 2020)

DNAprim

Secondary FlyBase IDs

FBgn0028834
FBgn0011425
FBgn0027911

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 41 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

44915

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VPH2

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VPH2

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VPH2

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

44915/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0259676

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0259676

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG5553

MARRVEL_MODEL - MARRVEL (model organism gene)

44915

Linkouts

BioGRID - A database of protein and genetic interactions.

69380

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0259676

DroID - A comprehensive database of gene and protein interactions.

FBgn0259676

DRSC - Results frm RNAi screens

FBgn0259676

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

DNApol-alpha60_1

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0259676

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5553

Flygut - An atlas of the Drosophila adult midgut

FBgn0259676

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0259676

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

DNA replication

MIST (protein-protein) - An integrated Molecular Interaction Database

44915

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (78)

The following external sites may use different assemblies or annotations than FlyBase.

0.03

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