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General Information
Symbol
Dmel\crb
Species
D. melanogaster
Name
crumbs
Annotation Symbol
CG6383
Feature Type
FlyBase ID
FBgn0259685
Gene Model Status
Stock Availability
Gene Snapshot
crumbs (crb) encodes a transmembrane protein that binds to multiple proteins such as those encoded by sdt, par-6, AP-2α, yrt, ex and Moe. It contributes to organization of zonula adherens, epithelial morphogenesis, apico-basal cell polarity, and is a negative regulator of Notch activity and growth control via the Hippo pathway. In photoreceptor cells it is involved in morphogenesis, the trafficking of the product of ninaE and prevention of light-dependent photoreceptor degeneration. [Date last reviewed: 2019-03-07]
Also Known As

l(3)07207, Crbs, l(3)j1B5, far

Key Links
Genomic Location
Cytogenetic map
Sequence location
3R:24,295,078..24,314,541 [+]
Recombination map

3-84

RefSeq locus
NT_033777 REGION:24295078..24314541
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Protein Family (UniProt)
Belongs to the Crumbs protein family. (P10040)
Molecular Function (GO)
[Detailed GO annotations]
Experimental Evidence
Predictions / Assertions
Summaries
Pathway (FlyBase)
Positive Regulators of Hippo Signaling Pathway -
The Hippo signaling pathway is an intracellular kinase cascade in which hpo kinase in complex with sav, phosphorylates wts kinase which, in turn, phosphorylates yki transcriptional co-activator leading to its cytosolic retention. Positive regulators of the pathway, enhance the cytosolic retention of yki, negatively regulating tissue growth (Adapted from FBrf0224870).
Negative Regulators of Notch Signaling Pathway -
The Notch receptor signaling pathway is activated by the binding of the transmembrane receptor Notch (N) to transmembrane ligands, Dl or Ser, presented on adjacent cells. This results in the proteolytic cleavage of N, releasing the intracellular domain (NICD). NICD translocates into the nucleus, interacting with Su(H) and mam to form a transcription complex, which up-regulates transcription of Notch-responsive genes. Negative regulators of the pathway down-regulate the signal from the sending cell or the response in the receiving cell. (Adapted from FBrf0225731 and FBrf0192604).
Protein Function (UniProtKB)
Plays a central role in cell polarity establishment (PubMed:2344615, PubMed:12900452, PubMed:10102271, PubMed:11740560). Participates in the assembly, positioning and maintenance of adherens junctions via its interaction with the SAC complex (PubMed:11740560, PubMed:12900452, PubMed:10102271, PubMed:11076972). Controls the coalescence of the spots of zonula adherens (ZA) into a adhesive ring around the cells (PubMed:11740560). It may act as a signal (PubMed:2344615). Involved in morphogenesis of the photoreceptor rhabdomere, for positioning and growth of rhabdomere and AJ during the crucial period of photoreceptor extension along the proximodistal axis of the retina (PubMed:12900452). Component of the crb-galla-Xpd (CGX) complex which is essential for proper mitotic chromosome segregation in early embryos (PubMed:25065591). The CGX complex is also required for cell proliferation in developing wing disks (PubMed:25065591). In the CGX complex, acts with galla-1 or galla-2 to recruit Xpd and thus form the functional complex. Together with apn, plays a key role in trachea development at larval stages (PubMed:30645584).
(UniProt, P10040)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
crb: crumbs
Homozygous lethal; many small holes in cuticle.
Summary (Interactive Fly)
Gene Model and Products
Number of Transcripts
4
Number of Unique Polypeptides
4

Please see the GBrowse view of Dmel\crb or the JBrowse view of Dmel\crb for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.47

Low-frequency RNA-Seq exon junction(s) not annotated.

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0084603
7543
2146
FBtr0111008
7672
2189
FBtr0304726
7973
2253
FBtr0334925
7714
2203
Additional Transcript Data and Comments
Reported size (kB)

7.7, 7.5 (northern blot)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0083987
233.6
2146
4.50
FBpp0110307
238.6
2189
4.53
FBpp0293268
244.7
2253
4.55
FBpp0306945
240.5
2203
4.58
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)

2139 (aa); 234 (kD)

Comments

crb protein is used as a marker for the boundary cells that delineate the dorsal and ventral domains of the embryonic large intestine.

Four Cys-poor regions of crb are distantly

related to domains present in the C-terminal part of laminin-A chains and

merosin. This homology suggests that the Cys-poor regions may be directly

involved in its role in epithelial organization.

External Data
Subunit Structure (UniProtKB)

Component of the SAC complex, a complex composed of crb, Patj and sdt (PubMed:11740560, PubMed:10102271, PubMed:11076972). May interact with the par-6 complex, which is composed of par-6, baz and aPKC, via its interaction with Patj (PubMed:12900452, PubMed:10102271, PubMed:11076972). Interacts with other proteins with Patj and sdt via its short cytoplasmic tail (PubMed:11740560). Component of the CGX complex composed of crb, galla (galla-1 or galla-2) and Xpd (PubMed:25065591). Able to interact independently (via intracellular domain) with galla-1, galla-2 and Xpd (PubMed:25065591). Interacts with apn (PubMed:30645584).

(UniProt, P10040)
Post Translational Modification

Phosphorylated in the cytoplasmic domain.

(UniProt, P10040)
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\crb using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (50 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR001881, InterPro:IPR018097
(assigned by InterPro )
inferred from biological aspect of ancestor with PANTHER:PTN002371879
(assigned by GO_Central )
traceable author statement
Biological Process (40 terms)
Terms Based on Experimental Evidence (40 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from mutant phenotype
inferred from physical interaction with FLYBASE:ex; FB:FBgn0004583
inferred from mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:ex; FB:FBgn0004583
inferred from genetic interaction with FLYBASE:Mer; FB:FBgn0086384
inferred from genetic interaction with FLYBASE:kibra; FB:FBgn0262127
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
(assigned by UniProt )
inferred from genetic interaction with FLYBASE:ex; FB:FBgn0004583
inferred from genetic interaction with FLYBASE:Mer; FB:FBgn0086384
inferred from genetic interaction with FLYBASE:kibra; FB:FBgn0262127
inferred from mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with UniProtKB:Q9VFX3
(assigned by UniProt )
inferred from mutant phenotype
inferred from mutant phenotype
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (6 terms)
Terms Based on Experimental Evidence (5 terms)
CV Term
Evidence
References
inferred from direct assay
(assigned by UniProt )
inferred from high throughput direct assay
inferred from high throughput direct assay
inferred from direct assay
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
traceable author statement
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
epithelial cell

Comment: clustered apical distribution

northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

crb transcripts are abundant in the apical region of tracheal cells from mid embryonic stage 11 until stage 14 and decrease afterwards.

Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
epithelial cell

Comment: clustered apical distribution

in situ
Stage
Tissue/Position (including subcellular localization)
Reference
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

crb protein is expressed in the apical membrane domain of the epithelial cells of imaginal discs and follicle cells in a reticular pattern outlining the borders of the cells.

crb protein is observed on the apical membranes of epithelial cells.

crb protein is used as a marker for the boundary cells that delineate the dorsal and ventral domains of the embryonic large intestine.

crb protein is detected in the scolopale cell lumen of the chordotonal organs.

crb protein localizes to the stalk of the rhabdomere. It is seen in this location from about 50% through pupal development only in rhabdomeres R2, R4, R5 and R7, because R1, R3 and R6 lack stalks at this stage.

Protein is observed concentrated in cells undergoing apical constriction during invagination of the salivary gland primordia.

The expression of crb protein is used as a marker for the apical surface of the hindgut epithelium. crb protein is detected outlining the lumina of the hidgut and malpighian tubules. crb protein is additionally detected in all ectodermally derived epithelia.

In third instar larvae, crb protein is observed in epithelial cells but not adepithelial cells of all imaginal discs. It is also seen in the inner and outer optic lobe anlage, the imaginal ring of the salivary glands, faintly in trachea, and on the surface of garland cells. In adult males, crb protein is observed in the testicular duct, seminal vesicle, male accessory gland, ejaculatory bulb, and ejaculatory duct. In females, it is observed in follicle cells in oogenesis stages S1-S10.

crb protein is first detected during gastrulation in the ectoderm and in the endoderm but not in the mesoderm. It localizes to the apical surfaces of epithelial cells and is preferentially concentrated at the borders between neighboring cells. Between stages 8 and 11 crb protein expression is lost in the posterior midgut, in the cells at the bottom of the stomodeal invagination, and in neuroblasts before they delaminate from the ectoderm. By stage 11, crb protein is restricted to epithelial cells of ectodermal origin and is observed in the foregut, the tracheal system, the salivary glands, the Malpighian tubules, the optic lobes, and the stomatogastric nervous system. Later in embryogenesis, expression is observed in the hindgut epithelium and in external sensory organs and chordotonal organs of the PNS.

Marker for
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
(assigned by UniProt )
inferred from high throughput direct assay
inferred from high throughput direct assay
inferred from direct assay
Expression Deduced from Reporters
Reporter: P{crb-lacZ.43.2}
Stage
Tissue/Position (including subcellular localization)
Reference
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\crb in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 70 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 57 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of crb
Transgenic constructs containing regulatory region of crb
Deletions and Duplications ( 17 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
dorsal mesothoracic disc & epithelial cell, with Scer\GAL4en-e16E
photoreceptor & adherens junction, with Scer\GAL4GMR.PF
zonula adherens & photoreceptor | somatic clone
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (41)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
6 of 15
Yes
Yes
 
5 of 15
No
Yes
3 of 15
No
No
 
2 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
 
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (39)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
5 of 15
Yes
Yes
5 of 15
Yes
Yes
2 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
 
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
 
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
Yes
Rattus norvegicus (Norway rat) (33)
4 of 13
Yes
Yes
4 of 13
Yes
Yes
2 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
Yes
1 of 13
No
No
1 of 13
No
No
1 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
Yes
1 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (11)
3 of 12
Yes
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
No
1 of 12
No
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
No
1 of 12
No
Yes
Danio rerio (Zebrafish) (39)
8 of 15
Yes
Yes
7 of 15
No
Yes
4 of 15
No
Yes
2 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (34)
10 of 15
Yes
Yes
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
Yes
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG0919006H )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150039 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W00B9 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X00AP )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Stegodyphus mimosarum
African social velvet spider
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G001C )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (15)
2 of 10
2 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 6 )
Potential Models Based on Orthology ( 3 )
Modifiers Based on Experimental Evidence ( 6 )
Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Interactions Browser

Please see the Physical Interaction reports below for full details
protein-protein
Physical Interaction
Assay
References
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement

Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Subunit Structure (UniProtKB)
Component of the SAC complex, a complex composed of crb, Patj and sdt (PubMed:11740560, PubMed:10102271, PubMed:11076972). May interact with the par-6 complex, which is composed of par-6, baz and aPKC, via its interaction with Patj (PubMed:12900452, PubMed:10102271, PubMed:11076972). Interacts with other proteins with Patj and sdt via its short cytoplasmic tail (PubMed:11740560). Component of the CGX complex composed of crb, galla (galla-1 or galla-2) and Xpd (PubMed:25065591). Able to interact independently (via intracellular domain) with galla-1, galla-2 and Xpd (PubMed:25065591). Interacts with apn (PubMed:30645584).
(UniProt, P10040 )
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
MIST (genetic) - An integrated Molecular Interaction Database
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Signaling Pathways (FlyBase)
Positive Regulators of Hippo Signaling Pathway -
The Hippo signaling pathway is an intracellular kinase cascade in which hpo kinase in complex with sav, phosphorylates wts kinase which, in turn, phosphorylates yki transcriptional co-activator leading to its cytosolic retention. Positive regulators of the pathway, enhance the cytosolic retention of yki, negatively regulating tissue growth (Adapted from FBrf0224870).
Negative Regulators of Notch Signaling Pathway -
The Notch receptor signaling pathway is activated by the binding of the transmembrane receptor Notch (N) to transmembrane ligands, Dl or Ser, presented on adjacent cells. This results in the proteolytic cleavage of N, releasing the intracellular domain (NICD). NICD translocates into the nucleus, interacting with Su(H) and mam to form a transcription complex, which up-regulates transcription of Notch-responsive genes. Negative regulators of the pathway down-regulate the signal from the sending cell or the response in the receiving cell. (Adapted from FBrf0225731 and FBrf0192604).
Metabolic Pathways
External Data
Linkouts
KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
Genomic Location and Detailed Mapping Data
Chromosome (arm)
3R
Recombination map

3-84

Cytogenetic map
Sequence location
3R:24,295,078..24,314,541 [+]
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
95F10-95F11
Limits computationally determined from genome sequence between P{PZ}crb07207&P{PZ}BRWD305842 and P{EP}CycB3EP3127
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
95F3-95F10
(determined by in situ hybridisation)
95F11-95F12
(determined by in situ hybridisation)
95F-95F
(determined by in situ hybridisation) 95F3--10 (determined by in situ hybridisation)
95F9-95F13
(determined by in situ hybridisation)
95F-95F
(determined by in situ hybridisation)
99F-99F
(determined by in situ hybridisation)
Experimentally Determined Recombination Data
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (24)
Genomic Clones (16)
 

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (30)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences
BDGP DGC clones
Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    BDGP DGC clones
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
    Commercially Available Antibodies
     
    Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
    Other Information
    Relationship to Other Genes
    Source for database identify of

    Source for identity of: crb CG6383

    Source for database merge of

    Source for merge of: crb l(3)S050920

    Source for merge of: crb l(3)j1B5

    Source for merge of: crb l(3)S058104

    Source for merge of: crb l(3)07207

    Source for merge of: crb far

    Additional comments

    Four more alleles discarded by Tubingen.

    Other Comments

    crb acts as a negative regulator of actomyosin dynamics during dorsal closure. Mutation of the crb FERM-domain binding motif affects elongation of the dorsal most epidermal cells, proper formation of the actomyosin cable at the leading edge and regulated constriction of amnioserosa cells.

    crb is required for cell polarity in the tubles only from the time when morphogenetic movements start.

    The crb 3' UTR is necessary and sufficient for it's apical localization in embryos.

    In vivo overexpression experiments reveal the kinase activity of aPKC on crb to be required for epithelial polarity.

    dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

    Overexpression of the JM region of the crb gene product recruits adherens junctions to ectopic sites of the photoreceptor cell membrane without causing loss of apicobasal polarity.

    crb is required to inhibit light-induced photoreceptor degeneration.

    crb is essential for photoreceptor morphogenesis. crb is required to maintain zonula adherens integrity during the rapid apical membrane expansion that builds the rhabdomere. In addition, crb regulates stalk development by stabilizing the membrane-associated spectrin cytoskeleton.

    sdt is a partner of crb in the control of epithelial cell polarity in the developing embryo.

    sdt interacts with crb to control polarity of epithelia but not neuroblasts.

    crb is required for the formation and maintenance of the follicular epithelium.

    Mutants exhibit cellular differentiation defects.

    Loss of cell polarity in the epidermal primordium of crb mutant embryos is associated with a failure to establish the zonulae adherentes. These junctions fail to develop when an altered polarity is induced by the overexpression of crb. crb and sdt have different functions during the formation of the zonula adherens.

    The autosomal "FLP-DFS" technique (using the P{ovoD1-18} P{FRT(whs)} P{hsFLP} chromosomes) has been used to identify the specific maternal effect phenotype for the zygotic lethal mutation. crb is required for germ cell viability or early oogenesis.

    crb and sdt are required for the third step in zonula adherens formation. The marginal zone of the apical membrane may contain a crb- and sdt-dependent retention mechanism for adherens junction material that facilitates zonula adherens formation.

    crb is part of the apical membrane and is concentrated in the immediate vicinity of the zonula adherens.

    The insertion of crb protein into the plasma memebrane is necessary and sufficient to confer apical character on a membrane domain. crb plays a key role in specifying the apical plasma membrane domain of ectodermal epithelial cells.

    Mutations in crb lead to widespread defects in the development of epithelial tissues followed by massive cell death during embryogenesis. Df(3L)W10 can block the massive cell death but the gross morphological defects are not rescued.

    crb and sdt encode critical components of a pathway that acts at the apical pole of epithelial cells to control their cytoarchitecture. Mosaic experiments suggest that sdt though not crb is required cell autonomously. Double mutant analysis suggests that sdt acts downstream of and is activated by crb.

    Cys poor regions of the proteins encoded by sli and ft are homologous with the laminin A-type modules of the crb protein.The laminin A-type modules of these proteins may participate in the interactions that control morphogenesis.

    Four Cys poor regions of crb are distantly related to domains present in the C terminal part of laminin A chains.

    The homology with the C terminal part of laminin A chains suggests that the Cys poor regions of crb may be directly involved in interactions organizing epithelia.

    Clonal analysis of crb indicates that crb is not cell autonomous in its expression, this suggests that the gene product may act as a diffusable factor and may serve as a signal in a cell-cell communication process.

    The crb gene encodes an integral membrane protein with 30 EGF-like repeats in the extracellular domain.

    crb may function to establish and/or maintain epithelia cell polarity.

    crb mutants display many small holes in the cuticle.

    Origin and Etymology
    Discoverer
    Etymology
    Identification
    External Crossreferences and Linkouts ( 68 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    FlyMine - An integrated database for Drosophila genomics
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    KEGG Genes - Molecular building blocks of life in the genomic space.
    modMine - A data warehouse for the modENCODE project
    SignaLink - A signaling pathway resource with multi-layered regulatory networks.
    Linkouts
    ApoDroso - Functional genomic database for photoreceptor development, survival and function
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Synonyms and Secondary IDs (20)
    Reported As
    Symbol Synonym
    Crb
    (Bonello and Peifer, 2019, Fahey-Lozano et al., 2019, McSharry and Beitel, 2019, Nunes de Almeida et al., 2019, Ogi et al., 2019, Wang et al., 2019, Fulford et al., 2018, Hong, 2018, Portela et al., 2018, Richardson and Portela, 2018, Chung et al., 2017, Hayashi and Dong, 2017, Lang and Munro, 2017, Perez-Mockus et al., 2017, Qiping et al., 2017, Richardson and Portela, 2017, Salis et al., 2017, Spannl et al., 2017, Flores-Benitez and Knust, 2016, Iwanami et al., 2016, Koch et al., 2016, Nguyen et al., 2016, Roman-Fernandez and Bryant, 2016, Silva et al., 2016, Walther et al., 2016, Bell et al., 2015, Cutler et al., 2015, Fletcher et al., 2015, Irvine and Harvey, 2015, Kumichel et al., 2015, Lin et al., 2015, Ohno et al., 2015, Satoh et al., 2015, Thompson and Sahai, 2015, Yeom et al., 2015, Amoyel and Bach, 2014, Chung and Andrew, 2014, Dong et al., 2014, Hall et al., 2014, Jiang et al., 2014, Pichaud, 2014, Sing et al., 2014, Skwarek et al., 2014, Thomas and Strutt, 2014, Chen and Zhang, 2013, Dong et al., 2013, Enderle and McNeill, 2013, Fox et al., 2013, Haack et al., 2013, Hombría and Sotillos, 2013, Ismat et al., 2013, Lucas et al., 2013, Pocha and Knust, 2013, Satoh et al., 2013, Song et al., 2013, Yu and Guan, 2013, Fausti et al., 2012, Fichelson et al., 2012, Fletcher et al., 2012, Liu et al., 2012, McKinley et al., 2012, Roper, 2012, Sarpal et al., 2012, Sen et al., 2012, Syed et al., 2012, Tepass, 2012, Zhai et al., 2012, Zhou and Hong, 2012, Chen et al., 2011, Genevet and Tapon, 2011, Gilbert et al., 2011, Halder and Johnson, 2011, Huang et al., 2011, Hwang and Rulifson, 2011, Laprise, 2011, Laprise and Tepass, 2011, Maruyama et al., 2011, Mathew et al., 2011, Shaik et al., 2011, Wang et al., 2011, Xiong and Rebay, 2011, Zhao et al., 2011, Bahri et al., 2010, Bulgakova et al., 2010, Chen et al., 2010, Colosimo et al., 2010, David et al., 2010, Duchi et al., 2010, Kaplan and Tolwinski, 2010, Maurel-Zaffran et al., 2010, Nelson et al., 2010, Nilton et al., 2010, Norum et al., 2010, Pirraglia et al., 2010, Simone and DiNardo, 2010, Tikhmyanova et al., 2010, Warner et al., 2010, Zhan et al., 2010, Chung et al., 2009, Genevet et al., 2009, Hamaratoglu et al., 2009, Kim et al., 2009, Martinez et al., 2009, Massarwa et al., 2009, Mirouse et al., 2009, Wang et al., 2009, Widmann and Dahmann, 2009, Woolworth et al., 2009, Zhang and Ward, 2009, Andrew and Baker, 2008, Bachmann et al., 2008, Bulgakova et al., 2008, Harris and Tepass, 2008, Laprise et al., 2008, Leibfried et al., 2008, Morrison et al., 2008, Nam et al., 2008, Pichaud, 2008.5.21, Steinberg et al., 2008, Vincent et al., 2008, de Velasco et al., 2007, Harris and Peifer, 2007, Hsouna et al., 2007, Kerman et al., 2007, Li et al., 2007, Martin et al., 2007, Morrison et al., 2007, Roeth et al., 2007, Tsarouhas et al., 2007, Wu et al., 2007, Abrams et al., 2006, Bayraktar et al., 2006, Lovegrove et al., 2006, Muschalik et al., 2006, Ng et al., 2006, Richard et al., 2006, Schneider et al., 2006, Suzuki and Ohno, 2006, Wang and Hartenstein, 2006, Beronja et al., 2005, Le Bivic, 2005, Lu and Bilder, 2005, Moberg et al., 2005, Wei et al., 2005, Wei et al., 2004, Harden et al., 2002, Pellikka et al., 2002)
    crb
    (Bajur et al., 2019, Fulford et al., 2019, Hartenstein et al., 2019, Lehmann et al., 2019, Silver et al., 2019, Skouloudaki et al., 2019, Snigdha et al., 2019, Alfred and Vaccari, 2018, Campbell et al., 2018, Das and Knust, 2018, de Vreede et al., 2018, Hochapfel et al., 2018, Saito et al., 2018, Tomaru et al., 2018, Wang et al., 2018, Hermle et al., 2017, Pellikka and Tepass, 2017, Ríos-Barrera et al., 2017, Salis et al., 2017, Su et al., 2017, Aigouy and Le Bivic, 2016, Bosch et al., 2016, Calero-Cuenca et al., 2016, Chung et al., 2016, Kockel et al., 2016, Nemetschke and Knust, 2016, Nguyen et al., 2016, Padash Barmchi et al., 2016, Sarov et al., 2016, Andersen et al., 2015, Doggett et al., 2015, Flores-Benitez and Knust, 2015, Francis and Ghabrial, 2015, Gene Disruption Project members, 2015-, Glassford et al., 2015, Kumichel et al., 2015, Sen et al., 2015, Sherrard and Fehon, 2015, Wang and Baker, 2015, Yeom et al., 2015, Zhang et al., 2015, Zhu et al., 2015, Andlauer et al., 2014, Ashwal-Fluss et al., 2014, Blanchard et al., 2014, Gamblin et al., 2014, Gurudev et al., 2014, Haltom et al., 2014, Kumichel and Knust, 2014, Nie et al., 2014, Ribeiro et al., 2014, Schottenfeld-Roames et al., 2014, Tipping and Perrimon, 2014, Aleksic et al., 2013, Firmino et al., 2013, Hombría and Sotillos, 2013, Kanda et al., 2013, Klose et al., 2013, Kwon et al., 2013, Letizia et al., 2013, Müller et al., 2013, Nakamura et al., 2013, Repiso et al., 2013, Saunders et al., 2013, Sotillos et al., 2013, Soukup et al., 2013, Steffensmeier et al., 2013, Wells et al., 2013, Chartier et al., 2012, Gomez et al., 2012, Hafezi et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Justiniano et al., 2012, Knust, 2012.7.1, Meier et al., 2012, Mishra et al., 2012, Penalva and Mirouse, 2012, Rodriguez et al., 2012, Wang et al., 2012, Zhou and Hong, 2012, Bao et al., 2011, Galy et al., 2011, Huang et al., 2011, Laprise, 2011, Letizia et al., 2011, Pocha et al., 2011, Pocha et al., 2011, Reddy and Irvine, 2011, Stümpges and Behr, 2011, Zhou et al., 2011, Bulgakova et al., 2010, Campbell et al., 2010, Chen et al., 2010, Fichelson et al., 2010, Forster et al., 2010, Franz and Riechmann, 2010, Garlena et al., 2010, Grzeschik et al., 2010, Hsouna et al., 2010, Kilic et al., 2010, Krahn et al., 2010, Laprise et al., 2010, Ling et al., 2010, Morais-de-Sá et al., 2010, Murthy et al., 2010, Richardson and Pichaud, 2010, Robinson et al., 2010, Shao et al., 2010, Walther and Pichaud, 2010, Campbell et al., 2009, Chanana et al., 2009, Chen et al., 2009, Gilbert et al., 2009, Gilbert et al., 2009, Huang et al., 2009, Jeon and Zinn, 2009, Kaplan et al., 2009, Leong et al., 2009, Richard et al., 2009, Roeth et al., 2009, Bachmann et al., 2008, Christensen et al., 2008.10.20, Denef et al., 2008, Herranz et al., 2008, Horne-Badovinac and Bilder, 2008, Huang et al., 2008, Kerman et al., 2008, Li et al., 2008, Li et al., 2008, Rivas et al., 2008, Sotillos et al., 2008, Steinberg et al., 2008, Xu et al., 2008, Blankenship et al., 2007, Georlette et al., 2007, Gilbert et al., 2007, Grzeschik et al., 2007, Koizumi et al., 2007, Kolsch et al., 2007, Lecuyer et al., 2007, Maeda et al., 2007, Sandmann et al., 2007, Szafranski and Goode, 2007, Wang and Riechmann, 2007, Banerjee et al., 2006, D'Costa et al., 2006, Giebel and Wodarz, 2006, Herranz et al., 2006, Laprise et al., 2006, Liebl et al., 2006, Lovegrove et al., 2006, Luschnig et al., 2006, Mohit et al., 2006, Nam and Choi, 2006, Wang et al., 2006, Budde, 2005, Harris and Peifer, 2005, Harris and Peifer, 2004, Fan et al., 2003, Myat and Andrew, 2002)
    l(3)S050920
    l(3)S058104
    Name Synonyms
    Crumbs
    (Campbell et al., 2019, Hong, 2018, Pichaud, 2018, Schmidt and Grosshans, 2018, Baker, 2017, Lang and Munro, 2017, Schopf and Huber, 2017, Barr et al., 2016, Chung et al., 2016, Clavería and Torres, 2016, Koch et al., 2016, Nemetschke and Knust, 2016, Nguyen et al., 2016, Veeman and McDonald, 2016, Whitney et al., 2016, Yadav et al., 2016, Bell et al., 2015, Cutler et al., 2015, Hariharan, 2015, Kumichel et al., 2015, Lin et al., 2015, Peterson and Krasnow, 2015, Sen et al., 2015, Vichas et al., 2015, Zhu et al., 2015, Amoyel and Bach, 2014, Andlauer et al., 2014, Chung and Andrew, 2014, Dong et al., 2014, Sing et al., 2014, Bardet et al., 2013, Bivic, 2013, Chen and Zhang, 2013, Coelho et al., 2013, Lucas et al., 2013, Rousso et al., 2013, Song et al., 2013, Soukup et al., 2013, Yu and Guan, 2013, Armbruster and Luschnig, 2012, Bossing et al., 2012, Fichelson et al., 2012, Förster and Luschnig, 2012, Ile et al., 2012, Mukherjee et al., 2012, Roper, 2012, Sarpal et al., 2012, Schottenfeld-Roames and Ghabrial, 2012, Zhou and Hong, 2012, Bao et al., 2011, Boggiano et al., 2011, Chan et al., 2011, Halder and Johnson, 2011, Huang et al., 2011, Letizia et al., 2011, Pocha et al., 2011, Shaik et al., 2011, Bahri et al., 2010, Bulgakova et al., 2010, David et al., 2010, Fernandes et al., 2010, Fox et al., 2010, Franz and Riechmann, 2010, Gandille et al., 2010, Garlena et al., 2010, Grzeschik et al., 2010, Kilic et al., 2010, Laprise et al., 2010, Lee et al., 2010, Murthy et al., 2010, Nilton et al., 2010, Norum et al., 2010, Richardson and Pichaud, 2010, Robinson et al., 2010, Simone and DiNardo, 2010, Walther and Pichaud, 2010, Warner et al., 2010, Campbell et al., 2009, Chung et al., 2009, Genevet et al., 2009, Hijazi et al., 2009, Jeon and Zinn, 2009, Laprise et al., 2009, Leong et al., 2009, Liu et al., 2009, Martinez et al., 2009, Massarwa et al., 2009, Maybeck and Röper, 2009, Mirouse et al., 2009, Richard et al., 2009, Roeth et al., 2009, Wang et al., 2009, Zhang and Ward, 2009, Andrew and Baker, 2008, Bachmann et al., 2008, Banerjee et al., 2008, Bulgakova et al., 2008, Denef et al., 2008, Harris and Tepass, 2008, Harris and Tepass, 2008, Hozumi et al., 2008, Huang et al., 2008, Jayaram et al., 2008, Kwon et al., 2008, Laprise et al., 2008, Leibfried et al., 2008, Li et al., 2008, Ma et al., 2008, Martinek et al., 2008, Morrison et al., 2008, Moyer and Jacobs, 2008, Nam et al., 2008, Pichaud, 2008.5.21, Sotillos et al., 2008, Steinberg et al., 2008, Vincent et al., 2008, Windler and Bilder, 2008, Bulgakova and Knust, 2007, Duchi et al., 2007, Kerman et al., 2007, Letizia et al., 2007, Li et al., 2007, Thomas et al., 2007, Tountas and Fortini, 2007, Wang and Riechmann, 2007, Xu et al., 2007, Abrams et al., 2006, Fehon, 2006, Fraichard et al., 2006, Humbert et al., 2006, Husain et al., 2006, Jaekel and Klein, 2006, Kempkens et al., 2006, Laprise et al., 2006, Nam and Choi, 2006, Ng et al., 2006, Pirraglia et al., 2006, Richard et al., 2006, Solecki et al., 2006, Staudt et al., 2006, Beronja et al., 2005, Langevin et al., 2005, Lu and Bilder, 2005, Margolis and Borg, 2005, Menon et al., 2005, Banyai and Patthy, 2004, Harris and Peifer, 2004, Geisbrecht and Montell, 2002, Gonzalez-Gaitan and Jackle, 2000)
    fat rhabdomere
    fat rhabdomeres
    lethal (3) 07207
    Secondary FlyBase IDs
    • FBgn0086905
    • FBgn0000368
    • FBgn0024518
    • FBgn0028125
    • FBgn0060827
    • FBgn0010919
    • FBgn0044351
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (782)