FlyBase Gene Report: Dmel\CG42366

General Information

Symbol

Dmel\CG42366

Species

D. melanogaster

Name

Annotation Symbol

CG42366

Feature Type

protein_coding_gene

FlyBase ID

FBgn0259712

Gene Model Status

Current

Stock Availability

9 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

MAK

Key Links

Genomic Location

Cytogenetic map

30F1-30F1

Sequence location

2L:9,925,161..9,930,378 [+]

Recombination map

2-38

RefSeq locus

NT_033779 REGION:9925161..9930378

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (9 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR017441

(InterPro Project Members, 2004-)

enables protein kinase activity

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR008271

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity with HGNC:6816

(Gene Ontology Curators, 2002-)

enables protein serine/threonine kinase activity

inferred from biological aspect of ancestor with PANTHER:PTN000622075

(GO Reference Genome Project, 2011-)

Biological Process (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in cilium assembly

inferred from biological aspect of ancestor with PANTHER:PTN001172286

(GO Reference Genome Project, 2011-)

involved_in intracellular signal transduction

inferred from biological aspect of ancestor with PANTHER:PTN000622075

(GO Reference Genome Project, 2011-)

involved_in intraciliary transport

inferred from biological aspect of ancestor with PANTHER:PTN001172286

(GO Reference Genome Project, 2011-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

is_active_in cilium

inferred from biological aspect of ancestor with PANTHER:PTN001172286

(GO Reference Genome Project, 2011-)

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000622075

(GO Reference Genome Project, 2011-)

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000622075

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

UNCLASSIFIED CMGC KINASES

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

UNCLASSIFIED CMGC KINASES -: This group comprises CMGC kinases that do not classify under other groups in FlyBase.

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\CG42366 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VL64)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.45

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

CG42366-RA

FBtr0299965

NM_164876

2717

706

CG42366-RB

FBtr0331633

NM_001273378

2545

703

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

CG42366-PA

FBpp0289242

78.1

706

9.57

FBpp0304023

77.8

703

9.62

M9PFE3

NP_001260307

AGB92842

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CG42366 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG42366_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.19

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\CG42366 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CG42366

View exonic expression by tissue for Dmel\CG42366

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34319/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0259712

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0259712

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0259712

Flygut - An atlas of the Drosophila adult midgut

FBgn0259712

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 1 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 6 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CG42366

Transgenic constructs containing regulatory region of CG42366

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 6 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

CG42366^GD10339

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

CG42366^GD10339

Other Phenotypes

Allele

some die during pupal stage, with Scer\GAL4^pnr-MD237

CG42366^GD10339

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (51)

Hsap\MAK

9 of 14

Yes

Hsap\CILK1

8 of 14

Yes

Hsap\MOK

3 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (29)

Rnor\Mak

9 of 14

Yes

Rnor\Cilk1

8 of 14

Yes

Rnor\Cdkl5

2 of 14

Rnor\Mok

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (30)

Mmus\Mak

10 of 14

Yes

Mmus\Cilk1

9 of 14

Yes

Mmus\Cdkl5

2 of 14

Mmus\Mok

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (29)

Xtro\cilk1

6 of 13

Yes

Xtro\mak

5 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (26)

Drer\mak

9 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (34)

Cele\dyf-5

9 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (20)

Agam\AgaP_AGAP010115

10 of 12

Yes

Agam\AgaP_AGAP002515

2 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (57)

Atha\AT4G19110

9 of 13

Yes

Atha\AT5G45430

9 of 13

Yes

Atha\MHK

8 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (16)

Scer\IME2

8 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (12)

Spom\pit1

9 of 12

Yes

Spom\mde3

8 of 12

Yes

Spom\dsk1

1 of 12

Spom\gsk3

1 of 12

Spom\ksp1

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Spom\spk1

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:CG42366. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (26)

bsk

3 of 13

CG8565

3 of 13

nmo

3 of 13

3 of 13

3 of 13

3 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Doa

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

sgg

1 of 13

Sik3

1 of 13

Srpk79D

1 of 13

SRPK

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 3 )

Human Ortholog

Disease

Evidence

References

MAK; male germ cell associated kinase

model of retinitis pigmentosa 62

IEA

(FlyBase, 2019-)

CILK1; ciliogenesis associated kinase 1

model of endocrine-cerebro-osteodysplasia syndrome

IEA

(FlyBase, 2019-)

model of juvenile myoclonic epilepsy 10

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

MAK; male germ cell associated kinase

9 of 14

154235

RETINITIS PIGMENTOSA 62; RP62

retinitis pigmentosa 62

CILK1; ciliogenesis associated kinase 1

8 of 14

612325

MOK; MOK protein kinase

3 of 14

605762

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Other Interaction Browsers

Summary of Genetic Interactions

esyN Network Diagram

Other Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0259712

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q9VL64

Class of Gene

nuclear_gene

(FlyBase Genome Annotators, 2008)

protein_coding_gene

(FlyBase Genome Annotators, 2008)

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-38

Cytogenetic map

30F1-30F1

Sequence location

2L:9,925,161..9,930,378 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

30F1-30F1

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-38

(FlyBase, 2016)

2-32.4

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (9)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

27294

y¹ v¹; P{TRiP.JF02599}attP2

35451

y¹ sc^* v¹ sev²¹; P{TRiP.GL00376}attP2

36112

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00987}attP2

NIG-Fly

GL00376

y¹ v¹; P{TRiP.GL00376}attP2/TM3, Sb¹

M2L-0089

y¹ w¹¹¹⁸; CG42366^SK1 P{neoFRT}40A/CyO-Tb

HMS00987

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00987}attP2

2LG-0068

y² VhaAC39-1^cho-1 v¹; P{U6-CG42366.NIG.sgRNA}attP40/CyO

VDRC

v108102

P{KK102619}VIE-260B

v25814

w¹¹¹⁸; P{GD10339}v25814

Genomic Clones (11)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (3)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

MIP05041

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0259712

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Relationship to Other Genes

Source for database merge of

Additional comments

Annotation CG31878 split in release 5.10 of the genome annotation. Sequences corresponding to the 5' end of release 5.9 annotation CG31878 have been merged with release 5.9 annotation CG31711 to create release 5.10 annotation CG42366. Sequences corresponding to the 3' portion of release 5.9 annotation CG31878 have been made into the separate CG42367 annotation in release 5.10.

(FlyBase Genome Annotators, 2008)

FlyBase curator comment: Correspondence with author Hongay revealed that 'IME2' = 'CG31711'.

(Hongay et al., 2008)

Source for split of: CG4588

(FlyBase Genome Annotators, 2002-2003)

Annotation CG4588 split into CG31711 and CG31878 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

CG31711

(Keleman et al., 2009.8.5, Cornman and Willis, 2008, Hongay et al., 2008, Haerty et al., 2007, Weber et al., 2005, FlyBase Genome Annotators, 2002-2003, Zhu, 1999.11)

CG31878

(Lebo et al., 2009, Weber et al., 2005)

CG42366

(National Institute of Genetics Fly Stocks, 2022-, Ostalé et al., 2021, Hall et al., 2019, Lehmann et al., 2019, Song et al., 2017, Ugrankar et al., 2015, Pogson et al., 2014, Ni et al., 2011.7.19, Perkins et al., 2009.8.10, FlyBase Genome Annotators, 2008)

CG4588

(Morrison et al., 2000)

IME2

(Hongay et al., 2008)

MAK

(Bjorklund et al., 2006, Morrison et al., 2000, Morrison et al., 2000)

Name Synonyms

Secondary FlyBase IDs

FBan0031711
FBan0031878
FBgn0051711
FBgn0051878

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 31 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34319

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VL64

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VL64

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34319/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0259712

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0259712

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG42366

MARRVEL_MODEL - MARRVEL (model organism gene)

34319

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0259712

DRSC - Results frm RNAi screens

FBgn0259712

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG42366_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0259712

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0259712

Flygut - An atlas of the Drosophila adult midgut

FBgn0259712

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC011548

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q9VL64

References (33)

The following external sites may use different assemblies or annotations than FlyBase.

-0.19

Report Sections