FlyBase Gene Report: Dmel\zld

General Information

Symbol

Dmel\zld

Species

D. melanogaster

Name

zelda

Annotation Symbol

CG12701

Feature Type

protein_coding_gene

FlyBase ID

FBgn0259789

Gene Model Status

Current

Stock Availability

14 publicly available

Gene Summary

zelda (zld) encodes a zinc finger transcription factor that functions in early blastoderm development. It works as a zygotic genome activator because it has a global role in regulating early expressed genes, such as genes essential for cellularization, sex determination and pattern formation. It is also required at later times in development. [Date last reviewed: 2019-03-21] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

vfl, vielfaltig, EP1344

Key Links

Genomic Location

Cytogenetic map

18F-18F

Sequence location

X:19,766,465..19,783,548 [-]

Recombination map

1-64

RefSeq locus

NC_004354 REGION:19766465..19783548

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (16 terms)

Molecular Function (7 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

enables chromatin loop anchoring activity

inferred from mutant phenotype

(Ogiyama et al., 2018)

enables nucleosome binding

inferred from mutant phenotype

(McDaniel et al., 2019)

enables protein binding

inferred from physical interaction with FLYBASE:bi; FB:FBgn0000179

(Giannios and Tsitilou, 2013)

enables sequence-specific DNA binding

inferred from mutant phenotype

(McDaniel et al., 2019)

inferred from direct assay

(Harrison et al., 2010)

enables transcription cis-regulatory region binding

inferred from direct assay

(Liang et al., 2008)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables DNA-binding transcription activator activity, RNA polymerase II-specific

inferred from biological aspect of ancestor with PANTHER:PTN002811836

(assigned by GO_Central )

(Gaudet et al., 2011)

enables RNA polymerase II cis-regulatory region sequence-specific DNA binding

inferred from biological aspect of ancestor with PANTHER:PTN002811836

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (7 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

involved_in cellularization

inferred from mutant phenotype

(Liang et al., 2008)

involved_in mitotic cell cycle

inferred from mutant phenotype

(Staudt et al., 2006)

involved_in positive regulation of gastrulation

inferred from mutant phenotype

(McDaniel et al., 2019)

involved_in positive regulation of gene expression

inferred from mutant phenotype

(McDaniel et al., 2019)

involved_in positive regulation of neuroblast proliferation

inferred from mutant phenotype

(Reichardt et al., 2018)

involved_in positive regulation of transcription, DNA-templated

inferred from direct assay

(Hamm et al., 2017, Liang et al., 2008)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in regulation of transcription by RNA polymerase II

inferred from biological aspect of ancestor with PANTHER:PTN002811836

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in nucleoplasm

inferred from direct assay

(Staudt et al., 2006)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN002811836

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

C2H2 ZINC FINGER TRANSCRIPTION FACTORS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

C2H2 ZINC FINGER TRANSCRIPTION FACTORS -: Zinc finger C2H2 transcription factors are sequence-specific DNA binding proteins that regulate transcription. They possess DNA-binding domains that are formed from repeated Cys₂His₂ zinc finger motifs. (Adapted from PMID:1835093, FBrf0220103 and FBrf0155739).

Summary (Interactive Fly)

zinc finger transcription factor - functions in the cellular blastoderm to activate many genes essential for cellularization, sex determination and pattern formation

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\zld for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.55

Gene model reviewed during 5.45

Gene model reviewed during 5.48

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.56

Sequence Ontology: Class of Gene

gene

gene_with_edited_transcript

(Graveley et al., 2011)

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

zld-RA

FBtr0074808

NM_134512

7562

1596

zld-RB

FBtr0074807

NM_167685

7217

1596

zld-RD

FBtr0307537

NM_001258840

8011

1373

zld-RF

FBtr0344733

NM_001258839

5919

1367

zld-RG

FBtr0345014

NM_001298537

7157

1596

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

zld-PA

FBpp0074577

170.3

1596

6.55

NP_608356

AAF49020

zld-PB

FBpp0074576

170.3

1596

6.55

NP_728304

AAN09519

zld-PD

FBpp0300192

146.1

1373

6.90

NP_001245769

AFH07481

zld-PF

FBpp0311065

145.3

1367

6.79

NP_001245768

AFH07480

zld-PG

FBpp0311265

170.3

1596

6.55

NP_001285466

AHN59936

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1596 aa isoforms: zld-PA, zld-PB, zld-PG

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\zld using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

vfl_1
vfl_2

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism | ubiquitous

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 1 -- 5

organism

(Liang et al., 2008)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

dorsal ectoderm

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 16

midline glial cell

(Pearson et al., 2012)

posterior midline glial cell

(Pearson et al., 2012)

embryonic stage 15 -- 16

VUM interneuron

(Pearson et al., 2012)

VUM motor neuron

(Pearson et al., 2012)

median neuroblast MNB interneuron

(Pearson et al., 2012)

embryonic stage 16

embryonic brain

(Pearson et al., 2012)

imaginal disc primordium

(Pearson et al., 2012)

embryonic stage 16 -- 17

embryonic/larval central nervous system

(Liang et al., 2008)

third instar larval stage

optic lobe neuroepithelial cell

(Wang et al., 2013)

wandering third instar larval stage

wing pouch | restricted

(Molnar et al., 2012)

wing hinge primordium | restricted

(Molnar et al., 2012)

adult stage | female

ovary

(Liang et al., 2008)

egg chamber

(Liang et al., 2008)

nurse cell

(Liang et al., 2008)

egg stage

unfertilized egg

(Liang et al., 2008)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

wing disc

Comment: isoforms zld-RD, zld-RB, zld-RF

(Giannios and Tsitilou, 2013)

organism | restricted

Comment: excludes wing discs; isoforms zld-RD, zld-RB, zld-RF

(Giannios and Tsitilou, 2013)

Additional Descriptive Data

zld isoforms zld-RB, zld-RD, and zld-RF can be detected by RT-PCR in wing discs, and in larval tissues other than wing discs; zld-RA is not detected at this stage.

(Giannios and Tsitilou, 2013)

The CNS and brain expression resemble nuclear dots, consistent with a probe detection of pre-spliced nuclear RNA corresponding to zld transcript zld-RD (FBtr0307537). In contrast, the imaginal disc expression appears to be cytoplasmic, likely corresponding to transcript zld-RB (FBtr0074807).

(Pearson et al., 2012)

During stage 12, both anterior midline glial cells and posterior midline glial cells continue to express zld transcript zld-RB, while two midline neurons express zld-RD. At stages 15 and 16, all zld-expressing cells express zld-RD, including iVUM4 (VUM interneuron), mVUM4 (VUM motor neuron), and median neuroblast MNB interneurons (MNB progeny).

(Pearson et al., 2012)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 4

organism

(Nien et al., 2011)

embryonic stage 5

organism | ubiquitous

(Pearson et al., 2012)

embryonic stage 11

embryonic brain

(Pearson et al., 2012)

embryonic/larval central nervous system

(Pearson et al., 2012)

embryonic stage 12

embryonic/larval central nervous system | medial

(Pearson et al., 2012)

embryonic/larval trachea

(Pearson et al., 2012)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 6

organism

(Harrison et al., 2010)

larval stage

wing disc

(Giannios and Tsitilou, 2013)

organism | restricted

Comment: excludes wing discs

(Giannios and Tsitilou, 2013)

Additional Descriptive Data

zld protein is detected in embryos as early as nuclear cycle 2, and appears to accumulate to higher levels in blastoderm embryos. Protein levels appears to increase substantially during the second hour of development, coincident with the activation of the zygotic genome.

(Nien et al., 2011)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in nucleoplasm

inferred from direct assay

(Staudt et al., 2006)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\zld in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\zld

View exonic expression by tissue for Dmel\zld

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0259789

DRscDB

32994/tissue=All

EMBL-EBI Single Cell Expression Atlas

FBgn0259789

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG12701-RB

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG12701

Flygut - An atlas of the Drosophila adult midgut

FBgn0259789

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 25 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 19 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of zld

Transgenic constructs containing regulatory region of zld

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(1)Exel6253

(Ryder, 2004.4.26)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

zld^G0225

lethal, with Scer\GAL4^pnr-MD237

zld^GD7803

lethal | embryonic stage

zld^G0427

lethal | embryonic stage | recessive

lethal | larval stage

zld^G0427

lethal | larval stage | recessive

lethal | male

zld²⁹⁴

lethal | maternal effect

zld^ZnF2

lethal | pharate adult stage

zld^G0353

lethal | pupal stage

zld^G0353

lethal | pupal stage, with Scer\GAL4^pnr-MD237

zld^GD7803

lethal | recessive

lethal - all die before end of pupal stage, with Scer\GAL4^bi-md653

zld^GD7803

lethal - all die during embryonic stage | germline clone

zld²⁹⁴

partially lethal - majority die

partially lethal - majority die (with zld²⁹⁴)

partially lethal - majority die (with zld^JAZ)

zld²⁹⁴

partially lethal - majority die (with zld^ZnF2)

zld²⁹⁴

partially lethal - majority die | maternal effect

zld^UASp.cHa

partially lethal - majority die | maternal effect | recessive | conditional

zld^CRY2

partially lethal - majority die | recessive

zld^G0225

partially lethal - majority live

zld²⁹⁴

partially lethal - majority live (with zld²⁹⁴)

partially lethal - majority live (with zld^EDD)

zld²⁹⁴

partially lethal - majority live (with zld^ZnF1)

zld²⁹⁴

viable

Sterility

Allele

female sterile

fertile

Other Phenotypes

Allele

abnormal mitotic cell cycle

zld^unspecified

abnormal mitotic cell cycle | embryonic stage, with Scer\GAL4^{VP16.mat.αTub67C}

zld^EP1344

abnormal neuroanatomy

zld^G0353

abnormal neuroanatomy, with Scer\GAL4^ftz.ng

zld^EP1344

abnormal size, with Scer\GAL4^en-e16E

zld^GL00394

abnormal size | adult stage

zld^ZnF2

decreased occurrence of cell division | larval stage, with Scer\GAL4^ase.PU

zld^{shRNA.all.UAS}

decreased occurrence of cell division | larval stage, with Scer\GAL4^wor.PA, Scer\GAL80^ase.PN

zld^{shRNA.all.UAS}

majority die during embryonic stage | maternal effect | recessive | conditional

zld^CRY2

some die during embryonic stage | germline clone

zld²⁹⁴

visible, with Scer\GAL4^ftz.ng

zld^EP1344

visible, with Scer\GAL4^sca-537.4

zld^EP1344

visible | adult stage

zld^ZnF2

Phenotype manifest in

Allele

axon, with Scer\GAL4^ftz.ng

zld^EP1344

central nervous system

dorsocentral bristle, with Scer\GAL4^sca-537.4

zld^EP1344

embryo | blastoderm stage

zld^unspecified

embryo | conditional

zld^CRY2

embryonic/first instar larval cuticle

zld^G0353

embryonic/larval hemocyte | third instar larval stage, with Scer\GAL4^Pxn.PS

zld^EY00172

embryonic/larval neuron

zld^G0353

embryonic/larval neuron, with Scer\GAL4^ftz.ng

zld^EP1344

embryonic head

eye

zld^ZnF2

furrow canal | embryonic stage | germline clone

zld²⁹⁴

nucleus | embryonic stage | germline clone

zld²⁹⁴

nucleus | pre-blastoderm stage

zld^unspecified

presumptive embryonic/larval central nervous system

zld^G0353

presumptive embryonic/larval muscle system

zld^G0353

presumptive embryonic/larval peripheral nervous system

zld^G0353

scutellar bristle, with Scer\GAL4^sca-537.4

zld^EP1344

secondary neuroblast | larval stage, with Scer\GAL4^ase.PU

zld^{shRNA.all.UAS}

secondary neuroblast | larval stage, with Scer\GAL4^wor.PA, Scer\GAL80^ase.PN

zld^{shRNA.all.UAS}

tormogen cell, with Scer\GAL4^sca-537.4

zld^EP1344

wing, with Scer\GAL4^bi-md653

zld^fl.UAS

wing | posterior, with Scer\GAL4^en-e16E

zld^GL00394

wing blade, with Scer\GAL4^ftz.ng

zld^EP1344

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (6)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\GFI1B

1 of 15

Yes

Hsap\ZBTB16

1 of 15

Yes

Hsap\ZNF71

1 of 15

Yes

Hsap\ZNF114

1 of 15

Yes

Hsap\ZNF485

1 of 15

Yes

Hsap\ZNF784

1 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (3)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Gfi1b

1 of 15

Yes

Mmus\Gzf1

1 of 15

Yes

Mmus\Zscan2

1 of 15

Yes

Rattus norvegicus (Norway rat) (7)

Rnor\Gfi1b

1 of 13

Yes

Rnor\Plagl2

1 of 13

Yes

Rnor\Prdm5

1 of 13

Yes

Rnor\Zbtb16

1 of 13

Yes

Rnor\Zfp2

1 of 13

Yes

Rnor\Zfp410

1 of 13

Yes

Rnor\Zscan2

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (1)

Xtro\gfi1b

1 of 12

Yes

Danio rerio (Zebrafish) (17)

Drer\LOC100536587

1 of 15

Yes

Drer\LOC101884037

1 of 15

Yes

Drer\LOC101885450

1 of 15

Yes

Drer\LOC103909095

1 of 15

Yes

Drer\LOC108183935

1 of 15

Yes

Drer\plagl2

1 of 15

Yes

Drer\plagx

1 of 15

Yes

Drer\si:cabz01069015.1

1 of 15

Yes

Drer\si:ch73-138e16.3

1 of 15

Yes

Drer\si:ch73-138e16.6

1 of 15

Yes

Drer\si:ch211-166g5.4

1 of 15

Yes

Drer\si:dkey-253d23.11

1 of 15

Yes

Drer\si:dkeyp-68b7.7

1 of 15

Yes

Drer\wu:fd06e11

1 of 15

Yes

Drer\zbtb16b

1 of 15

Yes

Drer\zbtb49

1 of 15

Yes

Drer\znf1001

1 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (6)

Cele\C27A12.2

1 of 15

Yes

Cele\lin-29

1 of 15

Yes

Cele\lsy-2

1 of 15

Yes

Cele\tbc-17

1 of 15

Yes

Cele\Y56A3A.28

1 of 15

Yes

Cele\zfp-2

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\ADR1

1 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\prz1

1 of 12

Yes

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091903BI )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA22861

Drosophila persimilis

Dper\GL20974

Drosophila persimilis

Dper\GL12930

Drosophila willistoni

Dwil\GK16344

Drosophila virilis

Dvir\GJ14817

Drosophila mojavensis

Dmoj\GI15246

Drosophila grimshawi

Dgri\GH24491

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091500QT )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17016437

Glossina morsitans

Tsetse fly

Gmor\GMOY002510

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_07038

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_04153

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0CGC )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\Bmo.10663

Danaus plexippus

Monarch butterfly

Dple\DPOGS212559-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL007774

Apis florea

Little honeybee

Aflo\3815793988

Apis mellifera

Western honey bee

Amel\GB52047

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP10339

Bombus terrestris

Buff-tailed bumblebee

Bter\15213057

Linepithema humile

Argentine ant

Lhum\LH19118

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0009e8

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG021639

Tribolium castaneum

Red flour beetle

Tcas\vfl

Pediculus humanus

Human body louse

Phum\PHUM397080

Rhodnius prolixus

Kissing bug

Rpro\RPRC000020

Cimex lectularius

Bed bug

Clec\CLEC012436

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI008256

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_01747

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0CE4 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR001098

Stegodyphus mimosarum

African social velvet spider

Smim\KFM81212.1

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_14900

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )

No non-Arthropod Metazoa orthologies identified

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (34)

Gene Symbol

Score

Source

Alignment

1 of 10

Cf2

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

erm

1 of 10

esg

1 of 10

ham

1 of 10

ich

1 of 10

Kah

1 of 10

MESR4

1 of 10

MTF-1

1 of 10

rgr

1 of 10

scrt

1 of 10

sna

1 of 10

sqz

1 of 10

wdn

1 of 10

wor

1 of 10

ZIPIC

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

RNA-protein

Physical Interaction

Assay

References

zld - brat

anti bait coimmunoprecipitation, quantitative reverse transcription pcr, electrophoretic mobility shift assay, autoradiography

(Reichardt et al., 2018)

zld - stau

anti bait coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

protein-protein

Physical Interaction

Assay

References

zld - Kah

two hybrid array

(Shokri et al., 2019)

zld - Ubx

anti tag coimmunoprecipitation, western blot, proximity-dependent biotin identification, Identification by mass spectrometry, anti tag western blot

(Carnesecchi et al., 2020)

zld - mirr

two hybrid array

(Shokri et al., 2019)

zld - pho

two hybrid array

(Shokri et al., 2019)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

brat

suppressible

zld

(Reichardt et al., 2018)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

59289

DroID - A comprehensive database of gene and protein interactions.

FBgn0259789

MIST (protein-protein) - An integrated Molecular Interaction Database

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-64

Cytogenetic map

18F-18F

Sequence location

X:19,766,465..19,783,548 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

18F-18F

Left limit from in situ hybridisation (FBrf0108529) Right limit from in situ hybridisation (FBrf0108529)

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

18F1-18F2

(determined by in situ hybridisation)

(Peter et al., 2002, Abdelilah-Seyfried et al., 2000, Schäfer et al., 1999.8.12)

18F-18F

(determined by in situ hybridisation)

(Schäfer et al., 1999.6.15, Schäfer et al., 1999.12.2)

Experimentally Determined Recombination Data

Location

1-64

(FlyBase, 2016)

1-62.7

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (14)

Bloomington

11912

w^67c23 P{lacW}zld^G0427/FM7c

11980

w^67c23 P{lacW}zld^G0353/FM7c

42166

y¹ w^* Mi{MIC}zld^MI06706

56725

y¹ w^* Mi{MIC}zld^MI12141

12231

w^67c23 P{lacW}zld^G0225/FM7c, B⁺

51350

w¹¹¹⁸; PBac{zld-GFP.FPTB}VK00033/TM3, Sb¹

15004

y¹ w^67c23 P{EPgy2}zld^EY00172/FM7c

Kyoto

111837

w^67c23 P{lacW}zld^G0427 P{neoFRT}19A/FM7c; P{ey-FLP.N}5

VDRC

v38706

w¹¹¹⁸; P{GD7803}v38706

v38707

w¹¹¹⁸; P{GD7803}v38707

v318812

PBac{fTRG01235.sfGFP-TVPTBF}VK00033

v341565

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD00646}attP40/CyO-GFP

More stocks available...

Genomic Clones (19)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (87)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

FI17950

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0259789

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0259789

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

32994

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Koromila et al., 2020, Giannios and Tsitilou, 2013, Nien et al., 2011, Harrison et al., 2010)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: vfl CG12701

(Staudt et al., 2006)

Source for identity of: zld vfl

(FlyBase, 2009-)

Source for database merge of

Source for merge of: vfl l(1)G0353 l(1)G0225 l(1)G0427 EP1344

(Staudt et al., 2006)

Additional comments

Renamed from 'vfl' ('vielfaltig') to 'zld' ('zelda') to reflect clear usage preference in the published literature.

(FlyBase, 2009-)

The vfl gene may have been derived from the CG11902 gene by retroposition.

(Pan and Zhang, 2009)

FlyBase curator comment: Correspondence with authors Liang and Rushlow revealed that 'zld' = 'CG12701'.

(Liang et al., 2008)

"l(1)G0353" may affect "CG12701".

(Peter et al., 2002)

"l(1)G0062" may affect "CG12701". "l(1)G0120" may affect "CG12701". "l(1)G0150" may affect "CG12701". "l(1)G0209" may affect "CG12701". "l(1)G0223" may affect "CG12701". "l(1)G0353" may affect "CG12701". "l(1)G0225" may affect "CG12701".

(Peter et al., 2002)

"l(1)G0225" may affect "CG12701".

(Peter et al., 2002)

"l(1)G0427" may affect "CG12701".

(Peter et al., 2002)

Other Comments

Origin and Etymology

Discoverer

Etymology

The fullname 'zelda' is itself an acronym for 'zinc finger early drosophila activator'.

(FlyBase, 2009-)

For reasons apparent from the mutant phenotype, the gene is named "vielfaltig" (German for versatile or manifold).

(Staudt et al., 2006)

Identification

External Crossreferences and Linkouts ( 52 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

32994

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0259789

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0259789

DRscDB

32994/tissue=All

EMBL-EBI Single Cell Expression Atlas

FBgn0259789

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

vfl_1
vfl_2

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG12701

Flygut - An atlas of the Drosophila adult midgut

FBgn0259789

FlyMine - An integrated database for Drosophila genomics

FBgn0259789

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

32994

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC014798

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG12701

MARRVEL_MODEL

32994

modMine - A data warehouse for the modENCODE project

FBgn0259789

Linkouts

BioGRID - A database of protein and genetic interactions.

59289

DroID - A comprehensive database of gene and protein interactions.

FBgn0259789

DRSC - Results frm RNAi screens

FBgn0259789

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG12701-RB

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0259789

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (protein-protein) - An integrated Molecular Interaction Database

Synonyms and Secondary IDs (21)

Reported As

Symbol Synonym

CG12701

(Sarov et al., 2016, Japanese National Institute of Genetics, 2012.5.21, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Pan and Zhang, 2009, Stofanko et al., 2008, Staudt et al., 2006)

EP1344

VFL

(Blatti et al., 2015, Cheng et al., 2013)

Vfl

(Hartmann et al., 2013, Kvon et al., 2012)

ZLD

(McDaniel et al., 2019, Lefebvre and Lécuyer, 2018, Li et al., 2014, Giannios and Tsitilou, 2013, Samee and Sinha, 2013)

Zelda

(Stathopoulos and Newcomb, 2020, Tsai et al., 2020, He et al., 2019, McCarthy et al., 2015, Sun et al., 2015, Levine, 2011)

Zld

(DeAngelis et al., 2021, Irizarry and Stathopoulos, 2021, Carnesecchi et al., 2020, Koromila et al., 2020, Schnepf et al., 2020, Basu et al., 2018, Chambers et al., 2017, Samee et al., 2015, Shin and Hong, 2015, Mannervik, 2014, Martinez et al., 2014, Xu et al., 2014, Giannios and Tsitilou, 2013, Saunders et al., 2013, Kvon et al., 2012, Nien et al., 2011, Tsurumi et al., 2011)

l(1)G0225

l(1)G0353

l(1)G0427

vfl

(Nadimpalli et al., 2015, dos Santos, 2014, Jiang and Singh, 2014, Rogers et al., 2014, Djiane et al., 2013, Kwon et al., 2013, Spokony, 2013.8.25, Sung et al., 2013, Wang et al., 2013, Yáñez-Cuna et al., 2012, Staudt et al., 2006)

zelda

(Connacher and Goldstrohm, 2021, Parniewska and Stocker, 2020, Eichhorn et al., 2016)

zld

(Onur et al., 2021, Winbush and Singh, 2021, Li and Hidalgo, 2020, Lv et al., 2020, Mahmud et al., 2020, Erceg et al., 2019, He et al., 2019, Kwasnieski et al., 2019, Matthews and White, 2019, Shokri et al., 2019, Tiwari et al., 2019, Haines and Eisen, 2018, Khajouei and Sinha, 2018, Lucas et al., 2018, Reichardt et al., 2018, Xie and Blankenship, 2018, Barr et al., 2017, Hamm et al., 2017, Hannon et al., 2017, Koenecke et al., 2017, Ribeiro et al., 2017, Levario et al., 2016, Sandler and Stathopoulos, 2016, Sandler and Stathopoulos, 2016, Zhang et al., 2016, Blythe and Wieschaus, 2015, Foo et al., 2014, Darbo et al., 2013, Kanodia et al., 2012, Pearson et al., 2012, Rushlow and Shvartsman, 2012, Harrison et al., 2011, Nien et al., 2011, Struffi et al., 2011, Tsurumi et al., 2011, Harrison et al., 2010, Liang et al., 2008, Liang et al., 2008)

Name Synonyms

CG12701

EP1344

Vielfaltig

(Kvon et al., 2012, Yáñez-Cuna et al., 2012)

ZELDA

(Samee and Sinha, 2013)

lethal (1) G0225

lethal (1) G0353

lethal (1) G0427

vielfaltig

(Wang et al., 2013, Staudt et al., 2006)

vielfaltig/Zelda

(Laver et al., 2013)

zelda

(Blythe and Wieschaus, 2015, Dahlberg et al., 2015, Fu et al., 2014, Paris et al., 2013, Tsurumi et al., 2011, Liang et al., 2008, Liang et al., 2008)

zinc-finger early Drosophila activator

(Liang et al., 2008)

Secondary FlyBase IDs

FBgn0028317
FBgn0031071
FBgn0040186
FBgn0027202
FBgn0043370

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (165)

Report Sections

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