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Gene Dmel\vfl

General Information
Symbol	Dmel\vfl	Species	D. melanogaster
Name	vielfaltig	Annotation symbol	CG12701
Feature type	protein_coding_gene	FlyBase ID	FBgn0259789
Gene Model Status	Current	Stock availability	8 publicly available
Also Known As	zld
Genomic Location
Chromosome (arm)	X	Recombination map
Cytogenetic map	18F-18F	Sequence location	X:19,660,978..19,677,682 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene vielfaltig is referred to in FlyBase by the symbol Dmel\vfl (CG12701, FBgn0259789). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: sequence-specific DNA binding; transcription regulatory region sequence-specific DNA binding. There is experimental evidence that it is involved in the biological process: mitotic cell cycle; positive regulation of transcription, DNA-dependent; cellularization. 13 alleles are reported. The phenotypes of these alleles are annotated with: multicellular structure; organ system subdivision; organ system; non-connected developing system; adult segment; thoracic segment; integumentary plate; anatomical group; eo support cell; cell part; embryonic/larval neuron. It has 4 annotated transcripts and 4 annotated polypeptides. Protein features are: Zinc finger, C2H2; Zinc finger, C2H2-like; Zinc finger, C2H2-type/integrase, DNA-binding; Zinc finger, double-stranded RNA binding. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of very low expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, larval trachea, adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1638370_s_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of vfl. Gene sequence location is X:19660978..19677682.
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	References Struffi et al., 2011, Development 138(19): 4291--4299
FB2012_01	Unknown in situ Sequence features BKN45062 HFA19486 BKN29940 References Nien et al., 2011, PLoS Genet. 7(10): e1002339 Controlled Vocabulary Terms organism embryonic/larval central nervous system ovary embryonic cycle 3 egg stage embryonic stage 4 adult stage embryonic cycle 9 embryonic stage 2 nurse cell embryonic stage 5 embryonic stage 3 embryonic cycle 6 embryonic stage 1 embryonic cycle 5 embryonic cycle 8 unfertilized egg egg chamber embryonic cycle 7 female embryonic cycle 4 embryonic stage 17 embryonic stage 16
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 18F-18F   Left limit from in situ hybridisation (FBrf0108529) Right limit from in situ hybridisation (FBrf0108529)
Experimentally Determined Cytological Location
Cytogenetic map Notes References 18F1-18F   (determined by in situ hybridisation)   (Peter et al., 2002) 18F1-18F2   (determined by in situ hybridisation)   (Abdelilah-Seyfried et al., 2000) 18F-18F   (determined by in situ hybridisation)   (Schaefer et al., 1999.12.2) 18F1-18F2   (determined by in situ hybridisation)   (Schaefer et al., 1999.8.12) 18F-18F   (determined by in situ hybridisation)   (Schaefer et al., 1999.6.15)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\vfl for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) vfl-RA FBtr0074808  NM_134512   6610   1596 vfl-RB FBtr0074807  NM_167685   7318   1596 vfl-RC FBtr0307536     5876   1367 vfl-RD FBtr0307537     7632   1373
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein vfl-PA   FBpp0074577   170.3   1596   6.55     NP_608356   AAF49020 vfl-PB   FBpp0074576   170.3   1596   6.55     NP_728304   AAN09519 vfl-PC   FBpp0300191   145.3   1367   6.79       vfl-PD   FBpp0300192   146.1   1373   6.90
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Zinc finger, C2H2 (IPR007087) Zinc finger, C2H2-type/integrase, DNA-binding (IPR013087) Zinc finger, C2H2-like (IPR015880) Zinc finger, double-stranded RNA binding (IPR022755)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA264457   AA438849   AA439154   AA540478   AE014298 AAF49020   AE014298 AAN09519   AI457060   AI546283   AI546377   AJ427017   AJ427021   AJ427024   AQ254766   AW943948   BF489330   BG638829   BI170776   BI353053   BI356574   BI569993   BI605728   BI605796   BP546901   BQ103735   BT010035 AAQ22504   BT099683   CO178320   CO188941   CO191055   CO265250   CO266605   CO268390   CO268404   CO268975   CO269362   CO274330   CO275527   CO276222   CO276386   CO294457   CO294740   CO294924   CO295001   CO297661   CO300639   CO301106   CO301648   CO310491   CO313991   CO314631   CO317218   CO325047   CO325681   CO327433   CO327809   CO330631   CO330835   CO331371   CO333264   CO333354   CO340728   CO340781   CO342024   CO344934   EC057182   EC059637   EC068683   EC071539   EC074256   EC079380   EC082492   EC083491   EC086361   EC088224   EC194576   EC198872   EC201000   EC202949   EC204119   EC207053   EC208540   EC210446   EC212897   EC216708   EC217462   EC218592   EC220412   EC222922   EC223151   EC228715   EC229791   EC232058   EC235887   EC236612   EC237676   EC239434   EC240468   EC240483   EC240590   EC247492   EC250836   EC250952   EC253221   EC253281   EC254664   EV590949   EV592884   EV593017   EV596109   EV596780   EV600202   GH744527   GH768833   GO254589
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q7YU17 Q9VWC6
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 5   organism   (Liang et al., 2008) embryonic stage 16 -- 17   embryonic/larval central nervous system   (Liang et al., 2008) adult stage | female   ovary   (Liang et al., 2008) egg chamber   (Liang et al., 2008) nurse cell   (Liang et al., 2008) egg stage   unfertilized egg   (Liang et al., 2008)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0259789    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0259789 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of very low expression. Peak expression observed within 00-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0259789 expression level Developmental Stage   Expression Level embryo 00-02hr    9973 embryo 02-04hr    16211 embryo 04-06hr    12751 embryo 06-08hr    10204 embryo 08-10hr    5442 embryo 10-12hr    1915 embryo 12-14hr    2019 embryo 14-16hr    4947 embryo 16-18hr    3428 embryo 18-20hr    2716 embryo 20-22hr    4487 embryo 22-24hr    2847 larva L1    1218 larva L2    409 larva L3 12hr old    390 larva L3 puffstage 1-2    466 larva L3 puffstage 3-6    1130 larva L3 puffstage 7-9    902 white prepupae new    795 white prepupae 12hr    494 white prepupae 24hr    706 pupae 2d postWPP    231 pupae 3d postWPP    64 pupae 4d postWPP    135 adult male 01day    70 adult male 05day    112 adult male 30day    173 adult female 01day    2676 adult female 05day    3910 adult female 30day    4434 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (9973) embryo 02-04hr  (16211) embryo 04-06hr  (12751) embryo 06-08hr  (10204) embryo 08-10hr  (5442) embryo 10-12hr  (1915) embryo 12-14hr  (2019) embryo 14-16hr  (4947) embryo 16-18hr  (3428) embryo 18-20hr  (2716) embryo 20-22hr  (4487) embryo 22-24hr  (2847) larva L1  (1218) larva L2  (409) larva L3 12hr old  (390) larva L3 puffstage 1-2  (466) larva L3 puffstage 3-6  (1130) larva L3 puffstage 7-9  (902) white prepupae new  (795) white prepupae 12hr  (494) white prepupae 24hr  (706) pupae 2d postWPP  (231) pupae 3d postWPP    64 pupae 4d postWPP  (135) adult male 01day    70 adult male 05day  (112) adult male 30day  (173) adult female 01day  (2676) adult female 05day  (3910) adult female 30day  (4434) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (9973) embryo 02-04hr  (16211) embryo 04-06hr  (12751) embryo 06-08hr  (10204) embryo 08-10hr  (5442) embryo 10-12hr  (1915) embryo 12-14hr  (2019) embryo 14-16hr  (4947) embryo 16-18hr  (3428) embryo 18-20hr  (2716) embryo 20-22hr  (4487) embryo 22-24hr  (2847) larva L1    1218 larva L2    409 larva L3 12hr old    390 larva L3 puffstage 1-2    466 larva L3 puffstage 3-6    1130 larva L3 puffstage 7-9    902 white prepupae new    795 white prepupae 12hr    494 white prepupae 24hr    706 pupae 2d postWPP    231 pupae 3d postWPP    64 pupae 4d postWPP    135 adult male 01day    70 adult male 05day    112 adult male 30day    173 adult female 01day  (2676) adult female 05day  (3910) adult female 30day  (4434) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    9973 embryo 02-04hr  (16211) embryo 04-06hr  (12751) embryo 06-08hr  10204 embryo 08-10hr    5442 embryo 10-12hr    1915 embryo 12-14hr    2019 embryo 14-16hr    4947 embryo 16-18hr    3428 embryo 18-20hr    2716 embryo 20-22hr    4487 embryo 22-24hr    2847 larva L1    1218 larva L2    409 larva L3 12hr old    390 larva L3 puffstage 1-2    466 larva L3 puffstage 3-6    1130 larva L3 puffstage 7-9    902 white prepupae new    795 white prepupae 12hr    494 white prepupae 24hr    706 pupae 2d postWPP    231 pupae 3d postWPP    64 pupae 4d postWPP    135 adult male 01day    70 adult male 05day    112 adult male 30day    173 adult female 01day    2676 adult female 05day    3910 adult female 30day    4434 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    9973 embryo 02-04hr    16211 embryo 04-06hr    12751 embryo 06-08hr    10204 embryo 08-10hr    5442 embryo 10-12hr    1915 embryo 12-14hr    2019 embryo 14-16hr    4947 embryo 16-18hr    3428 embryo 18-20hr    2716 embryo 20-22hr    4487 embryo 22-24hr    2847 larva L1    1218 larva L2    409 larva L3 12hr old    390 larva L3 puffstage 1-2    466 larva L3 puffstage 3-6    1130 larva L3 puffstage 7-9    902 white prepupae new    795 white prepupae 12hr    494 white prepupae 24hr    706 pupae 2d postWPP    231 pupae 3d postWPP    64 pupae 4d postWPP    135 adult male 01day    70 adult male 05day    112 adult male 30day    173 adult female 01day    2676 adult female 05day    3910 adult female 30day    4434 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0259789 expression level Developmental Stage   Expression Level embryo 00-02hr    9973 embryo 02-04hr    16211 embryo 04-06hr    12751 embryo 06-08hr    10204 embryo 08-10hr    5442 embryo 10-12hr    1915 embryo 12-14hr    2019 embryo 14-16hr    4947 embryo 16-18hr    3428 embryo 18-20hr    2716 embryo 20-22hr    4487 embryo 22-24hr    2847 larva L1    1218 larva L2    409 larva L3 12hr old    390 larva L3 puffstage 1-2    466 larva L3 puffstage 3-6    1130 larva L3 puffstage 7-9    902 white prepupae new    795 white prepupae 12hr    494 white prepupae 24hr    706 pupae 2d postWPP    231 pupae 3d postWPP    64 pupae 4d postWPP    135 adult male 01day    70 adult male 05day    112 adult male 30day    173 adult female 01day    2676 adult female 05day    3910 adult female 30day    4434 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (9973) embryo 02-04hr  (16211) embryo 04-06hr  (12751) embryo 06-08hr  (10204) embryo 08-10hr  (5442) embryo 10-12hr  (1915) embryo 12-14hr  (2019) embryo 14-16hr  (4947) embryo 16-18hr  (3428) embryo 18-20hr  (2716) embryo 20-22hr  (4487) embryo 22-24hr  (2847) larva L1  (1218) larva L2  (409) larva L3 12hr old  (390) larva L3 puffstage 1-2  (466) larva L3 puffstage 3-6  (1130) larva L3 puffstage 7-9  (902) white prepupae new  (795) white prepupae 12hr  (494) white prepupae 24hr  (706) pupae 2d postWPP  (231) pupae 3d postWPP    64 pupae 4d postWPP  135 adult male 01day    70 adult male 05day  112 adult male 30day  (173) adult female 01day  (2676) adult female 05day  (3910) adult female 30day  (4434) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (9973) embryo 02-04hr  (16211) embryo 04-06hr  (12751) embryo 06-08hr  (10204) embryo 08-10hr  (5442) embryo 10-12hr  1915 embryo 12-14hr  2019 embryo 14-16hr  (4947) embryo 16-18hr  (3428) embryo 18-20hr  (2716) embryo 20-22hr  (4487) embryo 22-24hr  (2847) larva L1    1218 larva L2    409 larva L3 12hr old    390 larva L3 puffstage 1-2    466 larva L3 puffstage 3-6    1130 larva L3 puffstage 7-9    902 white prepupae new    795 white prepupae 12hr    494 white prepupae 24hr    706 pupae 2d postWPP    231 pupae 3d postWPP    64 pupae 4d postWPP    135 adult male 01day    70 adult male 05day    112 adult male 30day    173 adult female 01day  (2676) adult female 05day  (3910) adult female 30day  (4434) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    9973 embryo 02-04hr  16211 embryo 04-06hr  12751 embryo 06-08hr  10204 embryo 08-10hr    5442 embryo 10-12hr    1915 embryo 12-14hr    2019 embryo 14-16hr    4947 embryo 16-18hr    3428 embryo 18-20hr    2716 embryo 20-22hr    4487 embryo 22-24hr    2847 larva L1    1218 larva L2    409 larva L3 12hr old    390 larva L3 puffstage 1-2    466 larva L3 puffstage 3-6    1130 larva L3 puffstage 7-9    902 white prepupae new    795 white prepupae 12hr    494 white prepupae 24hr    706 pupae 2d postWPP    231 pupae 3d postWPP    64 pupae 4d postWPP    135 adult male 01day    70 adult male 05day    112 adult male 30day    173 adult female 01day    2676 adult female 05day    3910 adult female 30day    4434 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    9973 embryo 02-04hr    16211 embryo 04-06hr    12751 embryo 06-08hr    10204 embryo 08-10hr    5442 embryo 10-12hr    1915 embryo 12-14hr    2019 embryo 14-16hr    4947 embryo 16-18hr    3428 embryo 18-20hr    2716 embryo 20-22hr    4487 embryo 22-24hr    2847 larva L1    1218 larva L2    409 larva L3 12hr old    390 larva L3 puffstage 1-2    466 larva L3 puffstage 3-6    1130 larva L3 puffstage 7-9    902 white prepupae new    795 white prepupae 12hr    494 white prepupae 24hr    706 pupae 2d postWPP    231 pupae 3d postWPP    64 pupae 4d postWPP    135 adult male 01day    70 adult male 05day    112 adult male 30day    173 adult female 01day    2676 adult female 05day    3910 adult female 30day    4434 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0259789    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0259789 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, larval trachea, adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0259789 expression level Tissue   Expression Level Larval Central Nervous System    557.85 Larval Midgut no informative data Larval Hindgut    50.9 Larval Malpighian Tubules    2.6 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    501.475 Larval Carcass    93.275 Adult Head    7 Adult Eye    4.5 Adult Brain    8.3 Adult Thoracic-Abdominal Ganglion    8.6 Adult Crop    29.2 Adult Midgut    2.3 Adult Hindgut    8.6 Adult Malpighian Tubules    8.6 Adult Fat Body no informative data Adult Salivary Gland    6.7 Adult Heart    1.725 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary    789.3 Adult Testis    18.1 Adult Male Accessory Gland    6.5 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (557.85) Larval Midgut no informative data Larval Hindgut    50.9 Larval Malpighian Tubules    2.6 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea  501.475 Larval Carcass    93.275 Adult Head    7 Adult Eye    4.5 Adult Brain    8.3 Adult Thoracic-Abdominal Ganglion    8.6 Adult Crop    29.2 Adult Midgut    2.3 Adult Hindgut    8.6 Adult Malpighian Tubules    8.6 Adult Fat Body no informative data Adult Salivary Gland    6.7 Adult Heart    1.725 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary  (789.3) Adult Testis    18.1 Adult Male Accessory Gland    6.5 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    557.85 Larval Midgut no informative data Larval Hindgut    50.9 Larval Malpighian Tubules    2.6 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    501.475 Larval Carcass    93.275 Adult Head    7 Adult Eye    4.5 Adult Brain    8.3 Adult Thoracic-Abdominal Ganglion    8.6 Adult Crop    29.2 Adult Midgut    2.3 Adult Hindgut    8.6 Adult Malpighian Tubules    8.6 Adult Fat Body no informative data Adult Salivary Gland    6.7 Adult Heart    1.725 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary    789.3 Adult Testis    18.1 Adult Male Accessory Gland    6.5 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    557.85 Larval Midgut no informative data Larval Hindgut    50.9 Larval Malpighian Tubules    2.6 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    501.475 Larval Carcass    93.275 Adult Head    7 Adult Eye    4.5 Adult Brain    8.3 Adult Thoracic-Abdominal Ganglion    8.6 Adult Crop    29.2 Adult Midgut    2.3 Adult Hindgut    8.6 Adult Malpighian Tubules    8.6 Adult Fat Body no informative data Adult Salivary Gland    6.7 Adult Heart    1.725 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary    789.3 Adult Testis    18.1 Adult Male Accessory Gland    6.5 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0259789 expression level Tissue   Expression Level Larval Central Nervous System    557.85 Larval Midgut no informative data Larval Hindgut    50.9 Larval Malpighian Tubules    2.6 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    501.475 Larval Carcass    93.275 Adult Head    7 Adult Eye    4.5 Adult Brain    8.3 Adult Thoracic-Abdominal Ganglion    8.6 Adult Crop    29.2 Adult Midgut    2.3 Adult Hindgut    8.6 Adult Malpighian Tubules    8.6 Adult Fat Body no informative data Adult Salivary Gland    6.7 Adult Heart    1.725 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary    789.3 Adult Testis    18.1 Adult Male Accessory Gland    6.5 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  557.85 Larval Midgut no informative data Larval Hindgut    50.9 Larval Malpighian Tubules    2.6 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea  501.475 Larval Carcass    93.275 Adult Head    7 Adult Eye    4.5 Adult Brain    8.3 Adult Thoracic-Abdominal Ganglion    8.6 Adult Crop    29.2 Adult Midgut    2.3 Adult Hindgut    8.6 Adult Malpighian Tubules    8.6 Adult Fat Body no informative data Adult Salivary Gland    6.7 Adult Heart    1.725 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary  789.3 Adult Testis    18.1 Adult Male Accessory Gland    6.5 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    557.85 Larval Midgut no informative data Larval Hindgut    50.9 Larval Malpighian Tubules    2.6 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    501.475 Larval Carcass    93.275 Adult Head    7 Adult Eye    4.5 Adult Brain    8.3 Adult Thoracic-Abdominal Ganglion    8.6 Adult Crop    29.2 Adult Midgut    2.3 Adult Hindgut    8.6 Adult Malpighian Tubules    8.6 Adult Fat Body no informative data Adult Salivary Gland    6.7 Adult Heart    1.725 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary    789.3 Adult Testis    18.1 Adult Male Accessory Gland    6.5 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    557.85 Larval Midgut no informative data Larval Hindgut    50.9 Larval Malpighian Tubules    2.6 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    501.475 Larval Carcass    93.275 Adult Head    7 Adult Eye    4.5 Adult Brain    8.3 Adult Thoracic-Abdominal Ganglion    8.6 Adult Crop    29.2 Adult Midgut    2.3 Adult Hindgut    8.6 Adult Malpighian Tubules    8.6 Adult Fat Body no informative data Adult Salivary Gland    6.7 Adult Heart    1.725 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary    789.3 Adult Testis    18.1 Adult Male Accessory Gland    6.5 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut no informative data Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body no informative data Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca no informative data Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass no informative data FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    7   NA  Eye    4.5   NA  Brain    8.3   557.85  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    8.6   NA  Crop    29.2   no informative data  Midgut    2.3   50.9  Hindgut    8.6   2.6  Malpighian Tubules    8.6   no informative data  Fat Body    no informative data   no informative data  Salivary Gland    6.7   NA  Heart    1.725   501.475  Trachea    NA   NA  VirginFemale Spermatheca    no informative data   NA  InseminatedFemale Spermatheca    no informative data   NA  Ovary    789.3   NA  Testis    18.1   NA  Male Accessory Gland    6.5   93.275  Carcass    no informative data modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_08
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0259789 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG12701-RB
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal vflG0225 lethal, with Scer\GAL4pnr-MD237 vflGD7803 lethal | embryonic stage vflG0427 lethal | embryonic stage | germline clone vfl294 lethal | embryonic stage | recessive vflG0353 vflG0427 lethal | larval stage vflG0427 lethal | larval stage | recessive vflG0353 vflG0427 lethal | pharate adult stage vflG0353 lethal | pupal stage vflG0353 lethal | pupal stage, with Scer\GAL4pnr-MD237 vflGD7803 lethal | recessive vflG0353 vflG0427 vflPG8 vflPL92 semi-lethal | recessive vflG0225 Other Phenotypes Allele mitotic cell cycle defective vflunspecified mitotic cell cycle defective | embryonic stage, with Scer\GAL4mat.αTub67C.T:Hsim\VP16 vflEP1344 neuroanatomy defective vflG0353 neuroanatomy defective, with Scer\GAL4ftz.ng vflEP1344 visible, with Scer\GAL4ftz.ng vflEP1344 visible, with Scer\GAL4sca-537.4 vflEP1344 Phenotype manifest in Allele axon, with Scer\GAL4ftz.ng vflEP1344 blastoderm embryo vflunspecified central nervous system vfl294 vfl681 dorsocentral bristle, with Scer\GAL4sca-537.4 vflEP1344 embryonic/first instar larval cuticle vflG0353 embryonic/larval neuron vflG0353 embryonic/larval neuron, with Scer\GAL4ftz.ng vflEP1344 embryonic head vfl294 vfl681 furrow canal | embryonic stage | germline clone vfl294 nucleus | embryonic stage | germline clone vfl294 nucleus | pre-blastoderm stage vflunspecified presumptive embryonic/larval central nervous system vflG0353 presumptive embryonic/larval muscle system vflG0353 presumptive embryonic/larval peripheral nervous system vflG0353 scutellar bristle, with Scer\GAL4sca-537.4 vflEP1344 tormogen cell, with Scer\GAL4sca-537.4 vflEP1344 wing blade, with Scer\GAL4ftz.ng vflEP1344
Classical Alleles ( 9 )
For All Classical Alleles Show
Allele of vfl Class Mutagen Stocks Known lesion vflG0353 P-element activity 2 Yes vflG0427 P-element activity 2 Yes vflG0225 P-element activity 1 Yes vfl294 amorphic allele - molecular evidence Delta2-3 0 Yes vfl681 amorphic allele - molecular evidence Delta2-3 0 Yes vflEP1344 P-element activity 0 Yes vflPG8 Delta2-3 0 -- vflPL92 Delta2-3 0 Yes vflunspecified 0 --
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of vfl Class Mutagen Stocks Known lesion vflGD7803 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes vflGL00394 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes vflotu.PL in vitro construct - regulatory fusion 0 Yes vflScer\UAS.cSa in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(1)Exel6253 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{TRiP.GL00394} NA P{UAS-vfl.S} NA characterization construct P{otu-vfl.L} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}EP1116 NA P{EP}vflEP1344 NA insertion of enhancer trap P{lacW}G0243a No P{lacW}l(1)G0120G0120 No P{lacW}l(1)G0150G0150 No P{lacW}l(1)G0209G0209 No P{lacW}l(1)G0223G0223 No P{lacW}vflG0225 No P{lacW}vflG0353 No P{lacW}vflG0427 No P{lacW}vflPL92 No insertion of enhancer trap binary system P{GawB}vflPG8 No miscellaneous insertions P{?RS3}vfl681 NA
Gene Ontology: Function, Process & Cellular Component ( 8 unique terms )
Terms Based on Experimental Evidence ( 7 terms )
Molecular Function
CV term Evidence References
sequence-specific DNA binding inferred from direct assay (Harrison et al., 2010) transcription regulatory region sequence-specific DNA binding inferred from direct assay (Liang et al., 2008)
Biological Process
CV term Evidence References
cellularization inferred from mutant phenotype (Liang et al., 2008) mitotic cell cycle inferred from mutant phenotype (Staudt et al., 2006) positive regulation of transcription, DNA-dependent inferred from direct assay (Liang et al., 2008)
Cellular Component
CV term Evidence References
colocalizes_with chromatin inferred from direct assay (Staudt et al., 2006) nucleoplasm inferred from direct assay (Staudt et al., 2006)
Terms Based on Predictions or Assertions ( 1 term )
Molecular Function
CV term Evidence References
zinc ion binding inferred from electronic annotation with InterPro:IPR007087, InterPro:IPR015880 (FlyBase Curators et al., 2004-)
Biological Process ( 0 terms)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	gene   nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions vfl allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 59289 DroID - A comprehensive database of gene and protein interactions. • FBgn0259789 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 32994
Orthologs
Genome-wide drosophilid orthologs	Dana\GF20463 Dere\GG18021 Dgri\GH24491 Dmoj\GI15246 Dper\GL20974 Dpse\GA22861 Dsec\GM22706 Dvir\GJ14817 Dwil\GK16344 Dyak\GE17353
Curated drosophilid orthologs	Dpse\GA11763
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AGAP002702-PA Anopheles gambiae (mosquito) XP_001121767.1 Apis mellifera (honey bee) BGIBMGA012283-PA Bombyx mori (silk worm) XP_001602590.1 Nasonia vitripennis (wasp) PHUM005031-PA Pediculus humanus (human body louse) PVX_084495 Plasmodium vivax (protozoal parasite)
Stocks & Reagents
Stocks Listed in FlyBase ( 8 )
Bloomington	11912 w67c23 P{lacW}vflG0427/FM7c 11980 w67c23 P{lacW}vflG0353/FM7c 12231 w67c23 P{lacW}vflG0225/FM7c, B+ 35468 y1 sc* v1; P{TRiP.GL00394}attP2
Kyoto	111837 w67c23 P{lacW}vflG0427 P{neoFRT}19A/FM7c; P{ey-FLP.N}5 111874 w67c23 P{lacW}vflG0353 P{neoFRT}19A/FM7c; P{ey-FLP.N}5
VDRC	v38706 w1118; P{GD7803}v38706 v38707 w1118; P{GD7803}v38707
Genomic Clones ( 2 )
	BACR33M08 BACR48P17 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 105 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD47819 RE29223
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT25308 GM22028 LD08959 LD13217 LD13635 LD19976 LD47938 RE12352 RE42753 RH02215 RH71622 RH71757
Other clones	12409 32533 36418 54568 57807 58764 67306 69992 76115 78302 78734 99430 108393 118311 121885 163100 192057 193047 214445.59 214445.60 214469.59 272783 281404 281770 CK01.7D.G3 CK01.8C.C6 CK01.8C.E3 CK01.10B.C3 CK01.10B.H11 CK01.12A.C4 CK01.12B.H10 CK01.52B.D10 CK01.62D.F12 CK01.71A.C7 CK01.117B.B12 CK01.119D.D12 CK01.119D.F11 CK01.123D.F7 CK01.147A.G9 CK01.147B.C3 CK01.167C.B12 Dmel_neb_007875_0600_2989 Dmel_neb_009675_0930_3358 Dmel_neb_009999_0378_1358 Dmel_neb_012092_0694_0951 Dmel_neb_013169_0570_2973 Dmel_neb_013687_0859_1515 EK042516 EK050556 EK056412 EK091224 EK091248 EK093222 EK094224 EK113822 EK117832 EK120027 EK120710 EK142028 EK147021 EK170309 EK178844 EK180061 EK181812 EK195449 EK197135 EK207005 EK207555 EK208001 EK208132 EK221835 EK234616 EK237310 EK267351 EK295761 EK296258 EK299423 EK306335 EK306559 EP06954 EP08056 EP11227 EP11337 EP13645 EP20801 FGH111C11 LD37284 RP01848 RP001016304 RP001054203 UUGC1146
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0259789 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32994 45115 47053 53492
Antibody Information
	polyclonal (Nien et al., 2011, Harrison et al., 2010)
Other Information
Discoverer
Etymology
	For reasons apparent from the mutant phenotype, the gene is named "vielfaltig" (German for versatile or manifold). (Staudt et al., 2006)
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: vfl CG12701 (Staudt et al., 2006)
Source for database merge of	Source for merge of: vfl l(1)G0353 l(1)G0225 l(1)G0427 EP1344 (Staudt et al., 2006)
Additional comments	FlyBase curator comment: Correspondence with authors Liang and Rushlow revealed that 'zld' = 'CG12701'. (Liang et al., 2008) "l(1)G0353" may affect "CG12701". (Peter et al., 2002) "l(1)G0062" may affect "CG12701". "l(1)G0120" may affect "CG12701". "l(1)G0150" may affect "CG12701". "l(1)G0209" may affect "CG12701". "l(1)G0223" may affect "CG12701". "l(1)G0353" may affect "CG12701". "l(1)G0225" may affect "CG12701". (Peter et al., 2002) "l(1)G0225" may affect "CG12701". (Peter et al., 2002) "l(1)G0427" may affect "CG12701". (Peter et al., 2002)
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_134512 NM_167685 RefSeq (Proteins) • NP_608356 NP_728304 DDBJ / EMBL / GenBank • AAF49020 AAN09519 AAQ22504 AI546283 AJ427017 AJ427021 AJ427024 AQ254766 AW943948 BT010035 Entrez Gene - A searchable database of RefSeq genes. • 32994 UniProtKB/TrEMBL • Q7YU17 Q9VWC6
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Zinc finger, C2H2 (IPR007087) Zinc finger, C2H2-type/integrase, DNA-binding (IPR013087) Zinc finger, C2H2-like (IPR015880) Zinc finger, double-stranded RNA binding (IPR022755)
Linkouts
	BioGRID - A database of protein and genetic interactions • 59289 DroID - A comprehensive database of gene and protein interactions. • FBgn0259789 DRSC - Results from RNAi screens. • FBgn0259789 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0259789 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG12701-RB FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0259789 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32994 45115 47053 53492 InParanoid A subset of ortholog calls from InParanoid. • AGAP002702-PA Anopheles gambiae (mosquito) XP_001121767.1 Apis mellifera (honey bee) BGIBMGA012283-PA Bombyx mori (silk worm) XP_001602590.1 Nasonia vitripennis (wasp) PHUM005031-PA Pediculus humanus (human body louse) PVX_084495 Plasmodium vivax (protozoal parasite) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /genebrief/vielfaltig_zelda.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 32994 modMine - Data generated by the modENCODE project. • FBgn0259789
Synonyms & Secondary IDs ( 14 )
Reported As
Symbol Synonym	CG12701 (Staudt et al., 2006, Stofanko et al., 2008, Ni et al., 2011.7.19) EP1344   l(1)G0225   l(1)G0353   l(1)G0427   vfl (Staudt et al., 2006) zld (Liang et al., 2008, Liang et al., 2008, Harrison et al., 2010)
Name Synonym	CG12701   EP1344   lethal (1) G0225   lethal (1) G0353   lethal (1) G0427   vielfaltig (Staudt et al., 2006) zelda (Liang et al., 2008, Liang et al., 2008) Zelda (Lusk and Eisen, 2010, Harrison et al., 2010) zinc-finger early Drosophila activator (Liang et al., 2008)
Secondary FlyBase IDs
	FBgn0027202 FBgn0028317 FBgn0031071 FBgn0040186 FBgn0043370
References ( 29 )
Generate a list of	All references relating to this gene ( 29 )
List References by type	Research paper  ( 11 ) Supplementary material  ( 4 ) Personal communication to FlyBase  ( 8 ) Abstract  ( 1 ) FlyBase analysis  ( 1 ) Computer file  ( 2 ) Automatic genome annotation  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 5 )
	Nien et al., 2011, PLoS Genet. 7(10): e1002339 Temporal coordination of gene networks by zelda in the early Drosophila embryo. [FBrf0216498] Struffi et al., 2011, Development 138(19): 4291--4299 Combinatorial activation and concentration-dependent repression of the Drosophila even skipped stripe 3+7 enhancer. [FBrf0215233] Tsurumi et al., 2011, PLoS Genet. 7(5): e1002086 STAT Is an Essential Activator of the Zygotic Genome in the Early Drosophila Embryo. [FBrf0214235] Harrison et al., 2010, Dev. Biol. 345(2): 248--255 Grainyhead and Zelda compete for binding to the promoters of the earliest-expressed Drosophila genes. [FBrf0211626] Lusk and Eisen, 2010, PLoS Genet. 6(1): e1000829 Evolutionary mirages: selection on binding site composition creates the illusion of conserved grammars in Drosophila enhancers. [FBrf0209873] Show all research papers ...