Gene Dmel\Nop60B

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General Information

Symbol

Dmel\Nop60B

Species

D. melanogaster

Name

Nucleolar protein at 60B

Annotation symbol

CG3333

Feature type

protein_coding_gene

FlyBase ID

FBgn0259937

Gene Model Status

Current

Stock availability

9 publicly available

Also Known As

mfl

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

60C1-60C2

Sequence location

2R:20,062,400..20,073,866 [+]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

The gene Nucleolar protein at 60B is referred to in FlyBase by the symbol Dmel\Nop60B (CG3333, FBgn0259937). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: pseudouridylate synthase activity. There is experimental evidence that it is involved in the biological process: wing disc development; ribosome biogenesis; germ cell development; rRNA processing; pseudouridine synthesis; neurogenesis. 21 alleles are reported. The phenotypes of these alleles are annotated with: organ system; adult segment; organ system subdivision; multicellular structure; thoracic segment; sense organ; eo support cell; adult; imaginal precursor; portion of tissue. It has 5 annotated transcripts and 5 annotated polypeptides. Protein features are: Dyskerin-like; PUA-like domain; Pseudouridine synthase II; Pseudouridine synthase, catalytic domain; Pseudouridine synthase/archaeosine transglycosylase; tRNA pseudouridine synthase B family. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed within 00-12 hour embryonic stages, during early larval stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at high levels in the following post-embryonic organs or tissues: larval trachea, adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, larval central nervous system, larval/adult midgut, larval hindgut, adult Malpighian tubules, adult heart, adult fat body, larval salivary gland, adult spermathecae, adult male accessory gland, larval/adult carcass. Gene sequence location is 2R:20062400..20073866.

User Contributed Data

Beta version, feedback welcome

External Summaries

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2013_03

FB2013_02

All updates

Click here to see a list of all updates to this record from FB2010_08 and on.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

60C1-60C2

Limits computationally determined from genome sequence between P{EP}EP503 and P{lacW}Nop60B^k05318

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

60B12-60C1

(Giordano, 1998.9.2)

60C1-60C2

(determined by in situ hybridisation)

(Spradling et al., 1999)

60B-60C

(determined by in situ hybridisation)

(Giordano et al., 1999)

60B13-60C1

(determined by in situ hybridisation)

(Phillips et al., 1998)

60C1-60C2

(Beaton, 1997.12.27)

60B12-60B13

60C1--2

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\Nop60B for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.42

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.48

3' exons correspond to Uhg6 (non-coding).

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Nop60B-RA

FBtr0072258

NM_080381

1753

508

Nop60B-RB

FBtr0100213

NM_001014547

2218

508

Nop60B-RC

FBtr0300227

NM_001169815

2184

508

Nop60B-RF

FBtr0300861

NM_001169818

3412

508

Nop60B-RG

FBtr0304599

NM_001259561

971

227

Additional Transcript Data & Comments

Reported size (kB)

~1.5 (northern blot); 1.7 (longest cDNA)

(Phillips et al., 1998)

2.0, 1.8 (northern blot)

(Giordano et al., 1999)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Nop60B-PA

FBpp0072166

56.8

508

9.88

NP_525120

AAF47178

Nop60B-PB

FBpp0099583

56.8

508

9.88

NP_001014547

AAX52682

Nop60B-PC

FBpp0289464

56.8

508

9.88

NP_001163286

ACZ94558

Nop60B-PF

FBpp0290083

56.8

508

9.88

NP_001163289

ACZ94561

Nop60B-PG

FBpp0293141

25.8

227

10.38

NP_001246490

AFH08243

Additional Polypeptide Data & Comments

Reported size (kDa)

508 (aa); 56 (kD predicted)

(Giordano et al., 1999)

508 (aa)

(Phillips et al., 1998)

Comments

Nop60B protein rescues the function of the yeast cbf5 gene.

(Phillips et al., 1998)

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

PUA-like domain (IPR015947)

Pseudouridine synthase/archaeosine transglycosylase (IPR002478)

Pseudouridine synthase II (IPR002501)

tRNA pseudouridine synthase B family (IPR004802)

Dyskerin-like (IPR012960)

Pseudouridine synthase, catalytic domain (IPR020103)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

O44081

UniProtKB/TrEMBL

E1JGV6

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Giordano et al., 1999)

embryonic stage 4 -- 14

organism | ubiquitous

(Phillips et al., 1998)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism | ubiquitous

(Phillips et al., 1998)

embryonic stage -- adult stage

(Giordano et al., 1999)

embryonic stage 1 -- 9

(Giordano et al., 1999)

Comment:reference states 0-4 hr AEL

oogenesis stage S3 -- S14 & adult stage | female

nurse cell

(Phillips et al., 1998)

oocyte

(Phillips et al., 1998)

oogenesis

ovary | ubiquitous

(Phillips et al., 1998)

ovary

(Giordano et al., 1999)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage & larval stage

(Giordano et al., 1999)

embryonic stage 1 -- 9

(Giordano et al., 1999)

Comment:reference states 0-4 hr AEL

embryonic stage 1 -- 11

(Giordano et al., 1999)

Comment:reference states 0-6 hr AEL

adult stage | female

(Giordano et al., 1999)

adult stage & oogenesis

nurse cell

(Giordano et al., 1999)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism | ubiquitous

(Phillips et al., 1998)

oogenesis

ovary

(Giordano et al., 1999)

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism | ubiquitous

(Phillips et al., 1998)

adult stage & oogenesis

germarium

(Phillips et al., 1998)

follicle cell

(Phillips et al., 1998)

oogenesis

organism | ubiquitous

(Phillips et al., 1998)

Additional Descriptive Data

Nop60B protein is localized to the nucleolus during interphase. During mitosis the protein is localized throughout the cytoplasm and is absent from the condensed chromosomes. With confocal microscopy, a low level of punctate nuclear staining is seen in addition to the nucleolar staining.

(Phillips et al., 1998)

Nop60B protein is detected in the nucleus of all cells during oogenesis and embryogenesis. In particular, Nop60B is detected in the nucleoli after cellularization occurs. The Nop60B polyclonal antibody also recognizes antigens localized to the nucleolous of yeast and rat cells.

(Phillips et al., 1998)

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

nucleolus

inferred from direct assay

(Giordano et al., 1999)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at high levels in the following post-embryonic organs or tissues: larval trachea, adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, larval central nervous system, larval/adult midgut, larval hindgut, adult Malpighian tubules, adult heart, adult fat body, larval salivary gland, adult spermathecae, adult male accessory gland, larval/adult carcass.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		62
central nervous system, larvae L3		57
central nervous system, pupae P8		11
head, virgin 1-day female		11
head, virgin 4-day female		14
head, virgin 20-day female		9
head, mated 1-day female		18
head, mated 4-day female		40
head, mated 20-day female		7
head, mated 1-day male		5
head, mated 4-day male		17
head, mated 20-day male		11
salivary gland, larvae L3 wandering		2
salivary gland, white prepupae		5
digestive system, larvae L3 wandering		16
digestive system, 1-day adult		23
digestive system, 4-day adult		68
digestive system, 20-day adult		29
fat body, larvae L3 wandering		5
fat body, white prepupae		11
fat body, pupae P8		19
carcass, larvae L3 wandering		50
carcass, 1-day adult		17
carcass, 4-day adult		48
carcass, 20-day adult		22
ovary, virgin 4-day female		343
ovary, mated 4-day female		370
testis, mated 4-day male		12
accessory gland, mated 4-day male		19
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(62)
central nervous system, larvae L3		(57)
central nervous system, pupae P8		11
head, virgin 1-day female		11
head, virgin 4-day female		14
head, virgin 20-day female		9
head, mated 1-day female		18
head, mated 4-day female		(40)
head, mated 20-day female		7
head, mated 1-day male		5
head, mated 4-day male		17
head, mated 20-day male		11
salivary gland, larvae L3 wandering		2
salivary gland, white prepupae		5
digestive system, larvae L3 wandering		16
digestive system, 1-day adult		23
digestive system, 4-day adult		(68)
digestive system, 20-day adult		(29)
fat body, larvae L3 wandering		5
fat body, white prepupae		11
fat body, pupae P8		19
carcass, larvae L3 wandering		(50)
carcass, 1-day adult		17
carcass, 4-day adult		(48)
carcass, 20-day adult		22
ovary, virgin 4-day female		(343)
ovary, mated 4-day female		(370)
testis, mated 4-day male		12
accessory gland, mated 4-day male		19
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		62
central nervous system, larvae L3		57
central nervous system, pupae P8		11
head, virgin 1-day female		11
head, virgin 4-day female		14
head, virgin 20-day female		9
head, mated 1-day female		18
head, mated 4-day female		40
head, mated 20-day female		7
head, mated 1-day male		5
head, mated 4-day male		17
head, mated 20-day male		11
salivary gland, larvae L3 wandering		2
salivary gland, white prepupae		5
digestive system, larvae L3 wandering		16
digestive system, 1-day adult		23
digestive system, 4-day adult		68
digestive system, 20-day adult		29
fat body, larvae L3 wandering		5
fat body, white prepupae		11
fat body, pupae P8		19
carcass, larvae L3 wandering		50
carcass, 1-day adult		17
carcass, 4-day adult		48
carcass, 20-day adult		22
ovary, virgin 4-day female		(343)
ovary, mated 4-day female		(370)
testis, mated 4-day male		12
accessory gland, mated 4-day male		19
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		62
central nervous system, larvae L3		57
central nervous system, pupae P8		11
head, virgin 1-day female		11
head, virgin 4-day female		14
head, virgin 20-day female		9
head, mated 1-day female		18
head, mated 4-day female		40
head, mated 20-day female		7
head, mated 1-day male		5
head, mated 4-day male		17
head, mated 20-day male		11
salivary gland, larvae L3 wandering		2
salivary gland, white prepupae		5
digestive system, larvae L3 wandering		16
digestive system, 1-day adult		23
digestive system, 4-day adult		68
digestive system, 20-day adult		29
fat body, larvae L3 wandering		5
fat body, white prepupae		11
fat body, pupae P8		19
carcass, larvae L3 wandering		50
carcass, 1-day adult		17
carcass, 4-day adult		48
carcass, 20-day adult		22
ovary, virgin 4-day female		343
ovary, mated 4-day female		370
testis, mated 4-day male		12
accessory gland, mated 4-day male		19
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		62
central nervous system, larvae L3		57
central nervous system, pupae P8		11
head, virgin 1-day female		11
head, virgin 4-day female		14
head, virgin 20-day female		9
head, mated 1-day female		18
head, mated 4-day female		40
head, mated 20-day female		7
head, mated 1-day male		5
head, mated 4-day male		17
head, mated 20-day male		11
salivary gland, larvae L3 wandering		2
salivary gland, white prepupae		5
digestive system, larvae L3 wandering		16
digestive system, 1-day adult		23
digestive system, 4-day adult		68
digestive system, 20-day adult		29
fat body, larvae L3 wandering		5
fat body, white prepupae		11
fat body, pupae P8		19
carcass, larvae L3 wandering		50
carcass, 1-day adult		17
carcass, 4-day adult		48
carcass, 20-day adult		22
ovary, virgin 4-day female		343
ovary, mated 4-day female		370
testis, mated 4-day male		12
accessory gland, mated 4-day male		19
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(62)
central nervous system, larvae L3		(57)
central nervous system, pupae P8		11
head, virgin 1-day female		11
head, virgin 4-day female		14
head, virgin 20-day female		9
head, mated 1-day female		18
head, mated 4-day female		(40)
head, mated 20-day female		7
head, mated 1-day male		5
head, mated 4-day male		17
head, mated 20-day male		11
salivary gland, larvae L3 wandering		2
salivary gland, white prepupae		5
digestive system, larvae L3 wandering		16
digestive system, 1-day adult		23
digestive system, 4-day adult		(68)
digestive system, 20-day adult		29
fat body, larvae L3 wandering		5
fat body, white prepupae		11
fat body, pupae P8		19
carcass, larvae L3 wandering		(50)
carcass, 1-day adult		17
carcass, 4-day adult		(48)
carcass, 20-day adult		22
ovary, virgin 4-day female		(343)
ovary, mated 4-day female		(370)
testis, mated 4-day male		12
accessory gland, mated 4-day male		19
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		62
central nervous system, larvae L3		57
central nervous system, pupae P8		11
head, virgin 1-day female		11
head, virgin 4-day female		14
head, virgin 20-day female		9
head, mated 1-day female		18
head, mated 4-day female		40
head, mated 20-day female		7
head, mated 1-day male		5
head, mated 4-day male		17
head, mated 20-day male		11
salivary gland, larvae L3 wandering		2
salivary gland, white prepupae		5
digestive system, larvae L3 wandering		16
digestive system, 1-day adult		23
digestive system, 4-day adult		68
digestive system, 20-day adult		29
fat body, larvae L3 wandering		5
fat body, white prepupae		11
fat body, pupae P8		19
carcass, larvae L3 wandering		50
carcass, 1-day adult		17
carcass, 4-day adult		48
carcass, 20-day adult		22
ovary, virgin 4-day female		(343)
ovary, mated 4-day female		(370)
testis, mated 4-day male		12
accessory gland, mated 4-day male		19
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		62
central nervous system, larvae L3		57
central nervous system, pupae P8		11
head, virgin 1-day female		11
head, virgin 4-day female		14
head, virgin 20-day female		9
head, mated 1-day female		18
head, mated 4-day female		40
head, mated 20-day female		7
head, mated 1-day male		5
head, mated 4-day male		17
head, mated 20-day male		11
salivary gland, larvae L3 wandering		2
salivary gland, white prepupae		5
digestive system, larvae L3 wandering		16
digestive system, 1-day adult		23
digestive system, 4-day adult		68
digestive system, 20-day adult		29
fat body, larvae L3 wandering		5
fat body, white prepupae		11
fat body, pupae P8		19
carcass, larvae L3 wandering		50
carcass, 1-day adult		17
carcass, 4-day adult		48
carcass, 20-day adult		22
ovary, virgin 4-day female		343
ovary, mated 4-day female		370
testis, mated 4-day male		12
accessory gland, mated 4-day male		19
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed within 00-12 hour embryonic stages, during early larval stages, in adult female stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		289
embryo 02-04hr		121
embryo 04-06hr		127
embryo 06-08hr		170
embryo 08-10hr		168
embryo 10-12hr		166
embryo 12-14hr		108
embryo 14-16hr		76
embryo 16-18hr		51
embryo 18-20hr		45
embryo 20-22hr		105
embryo 22-24hr		78
larva L1		177
larva L2		75
larva L3 12hr old		48
larva L3 puffstage 1-2		17
larva L3 puffstage 3-6		23
larva L3 puffstage 7-9		24
white prepupae new		20
white prepupae 12hr		16
white prepupae 24hr		14
pupae 2d postWPP		12
pupae 3d postWPP		6
pupae 4d postWPP		15
adult male 01day		5
adult male 05day		5
adult male 30day		6
adult female 01day		124
adult female 05day		200
adult female 30day		208
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(289)
embryo 02-04hr		(121)
embryo 04-06hr		(127)
embryo 06-08hr		(170)
embryo 08-10hr		(168)
embryo 10-12hr		(166)
embryo 12-14hr		(108)
embryo 14-16hr		(76)
embryo 16-18hr		(51)
embryo 18-20hr		(45)
embryo 20-22hr		(105)
embryo 22-24hr		(78)
larva L1		(177)
larva L2		(75)
larva L3 12hr old		(48)
larva L3 puffstage 1-2		17
larva L3 puffstage 3-6		23
larva L3 puffstage 7-9		24
white prepupae new		20
white prepupae 12hr		16
white prepupae 24hr		14
pupae 2d postWPP		12
pupae 3d postWPP		6
pupae 4d postWPP		15
adult male 01day		5
adult male 05day		5
adult male 30day		6
adult female 01day		(124)
adult female 05day		(200)
adult female 30day		(208)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		(289)
embryo 02-04hr		(121)
embryo 04-06hr		(127)
embryo 06-08hr		(170)
embryo 08-10hr		(168)
embryo 10-12hr		(166)
embryo 12-14hr		(108)
embryo 14-16hr		76
embryo 16-18hr		51
embryo 18-20hr		45
embryo 20-22hr		105
embryo 22-24hr		78
larva L1		(177)
larva L2		75
larva L3 12hr old		48
larva L3 puffstage 1-2		17
larva L3 puffstage 3-6		23
larva L3 puffstage 7-9		24
white prepupae new		20
white prepupae 12hr		16
white prepupae 24hr		14
pupae 2d postWPP		12
pupae 3d postWPP		6
pupae 4d postWPP		15
adult male 01day		5
adult male 05day		5
adult male 30day		6
adult female 01day		(124)
adult female 05day		(200)
adult female 30day		(208)
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		289
embryo 02-04hr		121
embryo 04-06hr		127
embryo 06-08hr		170
embryo 08-10hr		168
embryo 10-12hr		166
embryo 12-14hr		108
embryo 14-16hr		76
embryo 16-18hr		51
embryo 18-20hr		45
embryo 20-22hr		105
embryo 22-24hr		78
larva L1		177
larva L2		75
larva L3 12hr old		48
larva L3 puffstage 1-2		17
larva L3 puffstage 3-6		23
larva L3 puffstage 7-9		24
white prepupae new		20
white prepupae 12hr		16
white prepupae 24hr		14
pupae 2d postWPP		12
pupae 3d postWPP		6
pupae 4d postWPP		15
adult male 01day		5
adult male 05day		5
adult male 30day		6
adult female 01day		124
adult female 05day		200
adult female 30day		208
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		289
embryo 02-04hr		121
embryo 04-06hr		127
embryo 06-08hr		170
embryo 08-10hr		168
embryo 10-12hr		166
embryo 12-14hr		108
embryo 14-16hr		76
embryo 16-18hr		51
embryo 18-20hr		45
embryo 20-22hr		105
embryo 22-24hr		78
larva L1		177
larva L2		75
larva L3 12hr old		48
larva L3 puffstage 1-2		17
larva L3 puffstage 3-6		23
larva L3 puffstage 7-9		24
white prepupae new		20
white prepupae 12hr		16
white prepupae 24hr		14
pupae 2d postWPP		12
pupae 3d postWPP		6
pupae 4d postWPP		15
adult male 01day		5
adult male 05day		5
adult male 30day		6
adult female 01day		124
adult female 05day		200
adult female 30day		208
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(289)
embryo 02-04hr		(121)
embryo 04-06hr		(127)
embryo 06-08hr		(170)
embryo 08-10hr		(168)
embryo 10-12hr		(166)
embryo 12-14hr		(108)
embryo 14-16hr		(76)
embryo 16-18hr		(51)
embryo 18-20hr		(45)
embryo 20-22hr		(105)
embryo 22-24hr		(78)
larva L1		(177)
larva L2		(75)
larva L3 12hr old		(48)
larva L3 puffstage 1-2		17
larva L3 puffstage 3-6		23
larva L3 puffstage 7-9		24
white prepupae new		20
white prepupae 12hr		16
white prepupae 24hr		14
pupae 2d postWPP		12
pupae 3d postWPP		6
pupae 4d postWPP		15
adult male 01day		5
adult male 05day		5
adult male 30day		6
adult female 01day		(124)
adult female 05day		(200)
adult female 30day		(208)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		(289)
embryo 02-04hr		121
embryo 04-06hr		127
embryo 06-08hr		(170)
embryo 08-10hr		(168)
embryo 10-12hr		(166)
embryo 12-14hr		108
embryo 14-16hr		76
embryo 16-18hr		51
embryo 18-20hr		45
embryo 20-22hr		105
embryo 22-24hr		78
larva L1		(177)
larva L2		75
larva L3 12hr old		48
larva L3 puffstage 1-2		17
larva L3 puffstage 3-6		23
larva L3 puffstage 7-9		24
white prepupae new		20
white prepupae 12hr		16
white prepupae 24hr		14
pupae 2d postWPP		12
pupae 3d postWPP		6
pupae 4d postWPP		15
adult male 01day		5
adult male 05day		5
adult male 30day		6
adult female 01day		124
adult female 05day		(200)
adult female 30day		(208)
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		289
embryo 02-04hr		121
embryo 04-06hr		127
embryo 06-08hr		170
embryo 08-10hr		168
embryo 10-12hr		166
embryo 12-14hr		108
embryo 14-16hr		76
embryo 16-18hr		51
embryo 18-20hr		45
embryo 20-22hr		105
embryo 22-24hr		78
larva L1		177
larva L2		75
larva L3 12hr old		48
larva L3 puffstage 1-2		17
larva L3 puffstage 3-6		23
larva L3 puffstage 7-9		24
white prepupae new		20
white prepupae 12hr		16
white prepupae 24hr		14
pupae 2d postWPP		12
pupae 3d postWPP		6
pupae 4d postWPP		15
adult male 01day		5
adult male 05day		5
adult male 30day		6
adult female 01day		124
adult female 05day		200
adult female 30day		208
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		128
Schneider line 2 Sg4		192
embryonic 1182-4H		100
embryonic GM2		58
embryonic Kc167		106
embryonic S1		89
embryonic S3		204
leg disc CME L1		200
wing disc CME-W2		140
wing disc ML-DmD8		105
wing disc ML-DmD9		48
wing disc ML-DmD16-c3		120
wing disc ML-DmD21		186
wing disc ML-DmD32		92
haltere disc ML-DmD17-c3		129
eye-antennal disc ML-DmD11		83
antennal disc ML-DmD20-c5		128
mixed discs ML-DmD4-c1		73
CNS ML-DmBG1-c1		171
CNS ML-DmBG2-c2		67
tumorous blood cells mbn2		157
ovary fGS/OSS		83
ovary OSC		150
ovary OSS		76
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(128)
Schneider line 2 Sg4		(192)
embryonic 1182-4H		(100)
embryonic GM2		(58)
embryonic Kc167		(106)
embryonic S1		(89)
embryonic S3		(204)
leg disc CME L1		(200)
wing disc CME-W2		(140)
wing disc ML-DmD8		(105)
wing disc ML-DmD9		(48)
wing disc ML-DmD16-c3		(120)
wing disc ML-DmD21		(186)
wing disc ML-DmD32		(92)
haltere disc ML-DmD17-c3		(129)
eye-antennal disc ML-DmD11		(83)
antennal disc ML-DmD20-c5		(128)
mixed discs ML-DmD4-c1		(73)
CNS ML-DmBG1-c1		(171)
CNS ML-DmBG2-c2		(67)
tumorous blood cells mbn2		(157)
ovary fGS/OSS		(83)
ovary OSC		(150)
ovary OSS		(76)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		(128)
Schneider line 2 Sg4		(192)
embryonic 1182-4H		100
embryonic GM2		58
embryonic Kc167		106
embryonic S1		89
embryonic S3		(204)
leg disc CME L1		(200)
wing disc CME-W2		(140)
wing disc ML-DmD8		105
wing disc ML-DmD9		48
wing disc ML-DmD16-c3		(120)
wing disc ML-DmD21		(186)
wing disc ML-DmD32		92
haltere disc ML-DmD17-c3		(129)
eye-antennal disc ML-DmD11		83
antennal disc ML-DmD20-c5		(128)
mixed discs ML-DmD4-c1		73
CNS ML-DmBG1-c1		(171)
CNS ML-DmBG2-c2		67
tumorous blood cells mbn2		(157)
ovary fGS/OSS		83
ovary OSC		(150)
ovary OSS		76
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		128
Schneider line 2 Sg4		192
embryonic 1182-4H		100
embryonic GM2		58
embryonic Kc167		106
embryonic S1		89
embryonic S3		204
leg disc CME L1		200
wing disc CME-W2		140
wing disc ML-DmD8		105
wing disc ML-DmD9		48
wing disc ML-DmD16-c3		120
wing disc ML-DmD21		186
wing disc ML-DmD32		92
haltere disc ML-DmD17-c3		129
eye-antennal disc ML-DmD11		83
antennal disc ML-DmD20-c5		128
mixed discs ML-DmD4-c1		73
CNS ML-DmBG1-c1		171
CNS ML-DmBG2-c2		67
tumorous blood cells mbn2		157
ovary fGS/OSS		83
ovary OSC		150
ovary OSS		76
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		128
Schneider line 2 Sg4		192
embryonic 1182-4H		100
embryonic GM2		58
embryonic Kc167		106
embryonic S1		89
embryonic S3		204
leg disc CME L1		200
wing disc CME-W2		140
wing disc ML-DmD8		105
wing disc ML-DmD9		48
wing disc ML-DmD16-c3		120
wing disc ML-DmD21		186
wing disc ML-DmD32		92
haltere disc ML-DmD17-c3		129
eye-antennal disc ML-DmD11		83
antennal disc ML-DmD20-c5		128
mixed discs ML-DmD4-c1		73
CNS ML-DmBG1-c1		171
CNS ML-DmBG2-c2		67
tumorous blood cells mbn2		157
ovary fGS/OSS		83
ovary OSC		150
ovary OSS		76
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(128)
Schneider line 2 Sg4		(192)
embryonic 1182-4H		(100)
embryonic GM2		(58)
embryonic Kc167		(106)
embryonic S1		(89)
embryonic S3		(204)
leg disc CME L1		(200)
wing disc CME-W2		(140)
wing disc ML-DmD8		(105)
wing disc ML-DmD9		(48)
wing disc ML-DmD16-c3		(120)
wing disc ML-DmD21		(186)
wing disc ML-DmD32		(92)
haltere disc ML-DmD17-c3		(129)
eye-antennal disc ML-DmD11		(83)
antennal disc ML-DmD20-c5		(128)
mixed discs ML-DmD4-c1		(73)
CNS ML-DmBG1-c1		(171)
CNS ML-DmBG2-c2		(67)
tumorous blood cells mbn2		(157)
ovary fGS/OSS		(83)
ovary OSC		(150)
ovary OSS		(76)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		128
Schneider line 2 Sg4		(192)
embryonic 1182-4H		100
embryonic GM2		58
embryonic Kc167		106
embryonic S1		89
embryonic S3		(204)
leg disc CME L1		(200)
wing disc CME-W2		140
wing disc ML-DmD8		105
wing disc ML-DmD9		48
wing disc ML-DmD16-c3		120
wing disc ML-DmD21		(186)
wing disc ML-DmD32		92
haltere disc ML-DmD17-c3		129
eye-antennal disc ML-DmD11		83
antennal disc ML-DmD20-c5		128
mixed discs ML-DmD4-c1		73
CNS ML-DmBG1-c1		(171)
CNS ML-DmBG2-c2		67
tumorous blood cells mbn2		(157)
ovary fGS/OSS		83
ovary OSC		(150)
ovary OSS		76
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		128
Schneider line 2 Sg4		192
embryonic 1182-4H		100
embryonic GM2		58
embryonic Kc167		106
embryonic S1		89
embryonic S3		204
leg disc CME L1		200
wing disc CME-W2		140
wing disc ML-DmD8		105
wing disc ML-DmD9		48
wing disc ML-DmD16-c3		120
wing disc ML-DmD21		186
wing disc ML-DmD32		92
haltere disc ML-DmD17-c3		129
eye-antennal disc ML-DmD11		83
antennal disc ML-DmD20-c5		128
mixed discs ML-DmD4-c1		73
CNS ML-DmBG1-c1		171
CNS ML-DmBG2-c2		67
tumorous blood cells mbn2		157
ovary fGS/OSS		83
ovary OSC		150
ovary OSS		76
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		68
cold shock, 4-day adult		42
heat shock, 4-day adult		93
Cadmium 50 mM 6 hrs, larvae L3		25
Cadmium 50 mM 12 hrs, larvae L3		27
Cadmium 50 mM 48 hrs, 4-day adult		88
Cadmium 100 mM 48 hrs, 4-day adult		216
Copper 0.5 mM 12 hrs, larvae L3		27
Copper 15 mM 48 hrs, 4-day adult		261
Zinc 5 mM 12 hrs, larvae L3		25
Zinc 4.5 mM 48 hrs, 4-day adult		89
Ethanol 2.5% 3 hrs, larvae L3		166
Ethanol 5% 3 hrs, larvae L3		12
Ethanol 10% 3 hrs, larvae L3		29
Caffeine 1.5 mg/ml 4 hrs, larvae L3		10
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		184
Caffeine 25 mg/ml 48 hrs, 4-day adult		89
Paraquat 5 mM 48 hrs, 4-day adult		143
Paraquat 10 mM 48 hrs, 4-day adult		111
Rotenone 2 μg 12 hrs, larvae L3		34
Rotenone 8 μg 12 hrs, larvae L3		38
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		(68)
cold shock, 4-day adult		(42)
heat shock, 4-day adult		(93)
Cadmium 50 mM 6 hrs, larvae L3		25
Cadmium 50 mM 12 hrs, larvae L3		(27)
Cadmium 50 mM 48 hrs, 4-day adult		(88)
Cadmium 100 mM 48 hrs, 4-day adult		(216)
Copper 0.5 mM 12 hrs, larvae L3		(27)
Copper 15 mM 48 hrs, 4-day adult		(261)
Zinc 5 mM 12 hrs, larvae L3		25
Zinc 4.5 mM 48 hrs, 4-day adult		(89)
Ethanol 2.5% 3 hrs, larvae L3		(166)
Ethanol 5% 3 hrs, larvae L3		12
Ethanol 10% 3 hrs, larvae L3		(29)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		10
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(184)
Caffeine 25 mg/ml 48 hrs, 4-day adult		(89)
Paraquat 5 mM 48 hrs, 4-day adult		(143)
Paraquat 10 mM 48 hrs, 4-day adult		(111)
Rotenone 2 μg 12 hrs, larvae L3		(34)
Rotenone 8 μg 12 hrs, larvae L3		(38)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		68
cold shock, 4-day adult		42
heat shock, 4-day adult		93
Cadmium 50 mM 6 hrs, larvae L3		25
Cadmium 50 mM 12 hrs, larvae L3		27
Cadmium 50 mM 48 hrs, 4-day adult		88
Cadmium 100 mM 48 hrs, 4-day adult		(216)
Copper 0.5 mM 12 hrs, larvae L3		27
Copper 15 mM 48 hrs, 4-day adult		(261)
Zinc 5 mM 12 hrs, larvae L3		25
Zinc 4.5 mM 48 hrs, 4-day adult		89
Ethanol 2.5% 3 hrs, larvae L3		(166)
Ethanol 5% 3 hrs, larvae L3		12
Ethanol 10% 3 hrs, larvae L3		29
Caffeine 1.5 mg/ml 4 hrs, larvae L3		10
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(184)
Caffeine 25 mg/ml 48 hrs, 4-day adult		89
Paraquat 5 mM 48 hrs, 4-day adult		(143)
Paraquat 10 mM 48 hrs, 4-day adult		(111)
Rotenone 2 μg 12 hrs, larvae L3		34
Rotenone 8 μg 12 hrs, larvae L3		38
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		68
cold shock, 4-day adult		42
heat shock, 4-day adult		93
Cadmium 50 mM 6 hrs, larvae L3		25
Cadmium 50 mM 12 hrs, larvae L3		27
Cadmium 50 mM 48 hrs, 4-day adult		88
Cadmium 100 mM 48 hrs, 4-day adult		216
Copper 0.5 mM 12 hrs, larvae L3		27
Copper 15 mM 48 hrs, 4-day adult		261
Zinc 5 mM 12 hrs, larvae L3		25
Zinc 4.5 mM 48 hrs, 4-day adult		89
Ethanol 2.5% 3 hrs, larvae L3		166
Ethanol 5% 3 hrs, larvae L3		12
Ethanol 10% 3 hrs, larvae L3		29
Caffeine 1.5 mg/ml 4 hrs, larvae L3		10
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		184
Caffeine 25 mg/ml 48 hrs, 4-day adult		89
Paraquat 5 mM 48 hrs, 4-day adult		143
Paraquat 10 mM 48 hrs, 4-day adult		111
Rotenone 2 μg 12 hrs, larvae L3		34
Rotenone 8 μg 12 hrs, larvae L3		38
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		68
cold shock, 4-day adult		42
heat shock, 4-day adult		93
Cadmium 50 mM 6 hrs, larvae L3		25
Cadmium 50 mM 12 hrs, larvae L3		27
Cadmium 50 mM 48 hrs, 4-day adult		88
Cadmium 100 mM 48 hrs, 4-day adult		216
Copper 0.5 mM 12 hrs, larvae L3		27
Copper 15 mM 48 hrs, 4-day adult		261
Zinc 5 mM 12 hrs, larvae L3		25
Zinc 4.5 mM 48 hrs, 4-day adult		89
Ethanol 2.5% 3 hrs, larvae L3		166
Ethanol 5% 3 hrs, larvae L3		12
Ethanol 10% 3 hrs, larvae L3		29
Caffeine 1.5 mg/ml 4 hrs, larvae L3		10
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		184
Caffeine 25 mg/ml 48 hrs, 4-day adult		89
Paraquat 5 mM 48 hrs, 4-day adult		143
Paraquat 10 mM 48 hrs, 4-day adult		111
Rotenone 2 μg 12 hrs, larvae L3		34
Rotenone 8 μg 12 hrs, larvae L3		38
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		(68)
cold shock, 4-day adult		(42)
heat shock, 4-day adult		(93)
Cadmium 50 mM 6 hrs, larvae L3		25
Cadmium 50 mM 12 hrs, larvae L3		27
Cadmium 50 mM 48 hrs, 4-day adult		(88)
Cadmium 100 mM 48 hrs, 4-day adult		(216)
Copper 0.5 mM 12 hrs, larvae L3		27
Copper 15 mM 48 hrs, 4-day adult		(261)
Zinc 5 mM 12 hrs, larvae L3		25
Zinc 4.5 mM 48 hrs, 4-day adult		(89)
Ethanol 2.5% 3 hrs, larvae L3		(166)
Ethanol 5% 3 hrs, larvae L3		12
Ethanol 10% 3 hrs, larvae L3		29
Caffeine 1.5 mg/ml 4 hrs, larvae L3		10
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(184)
Caffeine 25 mg/ml 48 hrs, 4-day adult		(89)
Paraquat 5 mM 48 hrs, 4-day adult		(143)
Paraquat 10 mM 48 hrs, 4-day adult		(111)
Rotenone 2 μg 12 hrs, larvae L3		(34)
Rotenone 8 μg 12 hrs, larvae L3		(38)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		68
cold shock, 4-day adult		42
heat shock, 4-day adult		93
Cadmium 50 mM 6 hrs, larvae L3		25
Cadmium 50 mM 12 hrs, larvae L3		27
Cadmium 50 mM 48 hrs, 4-day adult		88
Cadmium 100 mM 48 hrs, 4-day adult		(216)
Copper 0.5 mM 12 hrs, larvae L3		27
Copper 15 mM 48 hrs, 4-day adult		(261)
Zinc 5 mM 12 hrs, larvae L3		25
Zinc 4.5 mM 48 hrs, 4-day adult		89
Ethanol 2.5% 3 hrs, larvae L3		(166)
Ethanol 5% 3 hrs, larvae L3		12
Ethanol 10% 3 hrs, larvae L3		29
Caffeine 1.5 mg/ml 4 hrs, larvae L3		10
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(184)
Caffeine 25 mg/ml 48 hrs, 4-day adult		89
Paraquat 5 mM 48 hrs, 4-day adult		143
Paraquat 10 mM 48 hrs, 4-day adult		111
Rotenone 2 μg 12 hrs, larvae L3		34
Rotenone 8 μg 12 hrs, larvae L3		38
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		68
cold shock, 4-day adult		42
heat shock, 4-day adult		93
Cadmium 50 mM 6 hrs, larvae L3		25
Cadmium 50 mM 12 hrs, larvae L3		27
Cadmium 50 mM 48 hrs, 4-day adult		88
Cadmium 100 mM 48 hrs, 4-day adult		216
Copper 0.5 mM 12 hrs, larvae L3		27
Copper 15 mM 48 hrs, 4-day adult		261
Zinc 5 mM 12 hrs, larvae L3		25
Zinc 4.5 mM 48 hrs, 4-day adult		89
Ethanol 2.5% 3 hrs, larvae L3		166
Ethanol 5% 3 hrs, larvae L3		12
Ethanol 10% 3 hrs, larvae L3		29
Caffeine 1.5 mg/ml 4 hrs, larvae L3		10
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		184
Caffeine 25 mg/ml 48 hrs, 4-day adult		89
Paraquat 5 mM 48 hrs, 4-day adult		143
Paraquat 10 mM 48 hrs, 4-day adult		111
Rotenone 2 μg 12 hrs, larvae L3		34
Rotenone 8 μg 12 hrs, larvae L3		38
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010, Graveley et al., 2011)

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

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FBgn0259937

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal

lethal, with Scer\GAL4^Act5C.PI

Nop60B^{dsRNA.sp.Scer\UAS}

lethal | embryonic stage | recessive

lethal | larval stage, with Scer\GAL4^Act5C.PU

Nop60B^GD16822

lethal | larval stage, with Scer\GAL4^ap-md544

Nop60B^GD16822

lethal | larval stage | recessive

lethal | pupal stage | recessive

Nop60B^k06308

lethal | recessive

partially lethal - majority die | pupal stage, with Scer\GAL4^pnr-MD237

Nop60B^GD10940

viable

viable, with Scer\GAL4^pnr-MD237

Nop60B^GD16822

Sterility

Allele

female fertile | reduced, with Scer\GAL4^Act5C.PI

Nop60B^{dsRNA.Sym.Scer\UAS}

female sterile | partially | recessive

Nop60B¹

male sterile

male sterile (with Nop60B^DL2011)

Nop60B^mM176

male sterile (with Nop60B^mM176)

Nop60B^DL2011

sterile

Nop60B^DL2011

sterile (with In(2LR)Px⁴)

Nop60B^DL2011

sterile (with Nop60B^DL3)

Nop60B^DL2011

sterile (with Nop60B^DL4)

Nop60B^DL2011

sterile (with Nop60B^DL6)

Nop60B^DL2011

sterile (with Nop60B^DL10)

Nop60B^DL2011

sterile (with Nop60B^DL2011)

Other Phenotypes

Allele

decreased cell size, with Scer\GAL4^nub-AC-62

Nop60B^GD16822

developmental rate defective, with Scer\GAL4^Act5C.PI

Nop60B^{dsRNA.Sym.Scer\UAS}

developmental rate defective | recessive

Nop60B¹

increased cell death | recessive

Nop60B¹

increased cell death | third instar larval stage, with Scer\GAL4^ap-md544

Nop60B^GD16822

increased cell death | third instar larval stage, with Scer\GAL4^en.PU

Nop60B^GD16822

melanotic mass phenotype | larval stage, with Scer\GAL4^Act5C.PU

Nop60B^GD16822

neuroanatomy defective | larval stage

small body | larval stage | recessive

visible, with Scer\GAL4^Act5C.PI

Nop60B^{dsRNA.Sym.Scer\UAS}

visible, with Scer\GAL4^Act5C.PU

Nop60B^GD16822

visible, with Scer\GAL4^Bx-MS1096

Nop60B^GD16822

visible, with Scer\GAL4^en.PU

Nop60B^GD16822

visible, with Scer\GAL4^nub-AC-62

Nop60B^GD16822

visible, with Scer\GAL4^pnr-MD237

visible, with Scer\GAL4^vg.int2.1

Nop60B^GD16822

visible | conditional | somatic clone

Nop60B^k05318

visible | recessive

Nop60B¹

Phenotype manifest in

Allele

adult abdomen & macrochaeta

Nop60B¹

adult cuticle & adult abdomen

Nop60B¹

adult cuticle & adult abdomen, with Scer\GAL4^Act5C.PI

Nop60B^{dsRNA.Sym.Scer\UAS}

adult thorax, with Scer\GAL4^pnr-MD237

Nop60B^{dsRNA.sp.Scer\UAS}

chaeta, with Scer\GAL4^Act5C.PI

Nop60B^{dsRNA.Sym.Scer\UAS}

chaeta, with Scer\GAL4^pnr-MD237

egg

Nop60B¹

egg chamber

Nop60B¹

embryo

Nop60B¹

embryonic/larval neuroblast | larval stage

ganglion mother cell | larval stage

germline cell | adult stage | male

Nop60B^DL4

hub cell

Nop60B^DL4

male germline stem cell

Nop60B^DL4

mesothoracic tergum, with Scer\GAL4^pnr-MD237

nurse cell & nucleus

Nop60B¹

spermatocyte fusome

Nop60B^DL4

testis

trichogen cell, with Scer\GAL4^pnr-MD237

Nop60B^GD10940

wing, with Scer\GAL4^ap-md544

Nop60B^GD16822

wing, with Scer\GAL4^nub-AC-62

Nop60B^GD16822

wing | conditional | somatic clone

Nop60B^k05318

wing blade, with Scer\GAL4^vg.int2.1

Nop60B^GD16822

wing disc, with Scer\GAL4^ap-md544

Nop60B^GD16822

wing disc, with Scer\GAL4^en.PU

Nop60B^GD16822

wing margin, with Scer\GAL4^Bx-MS1096

Nop60B^GD16822

wing margin, with Scer\GAL4^en.PU

Nop60B^GD16822

wing margin, with Scer\GAL4^vg.int2.1

Nop60B^GD16822

wing margin bristle, with Scer\GAL4^en.PU

Nop60B^GD16822

wing margin bristle, with Scer\GAL4^vg.int2.1

Nop60B^GD16822

wing vein, with Scer\GAL4^en.PU

Nop60B^GD16822

wing vein, with Scer\GAL4^nub-AC-62

Nop60B^GD16822

Classical Alleles ( 14 )

For All Classical Alleles Show

Allele of Nop60B	Class	Mutagen	Stocks	Known lesion
Nop60B^k05318		P-element activity	2	Yes
Nop60B^f01318		piggyBac transposase	1	--
Nop60B^k06308		P-element activity	1	Yes
Nop60B¹	hypomorphic allele - genetic evidence		0	Yes
Nop60B²		Delta2-3	0	Yes
Nop60B³		Delta2-3	0	Yes
Nop60B⁴		Delta2-3	0	Yes
Nop60B⁵		Delta2-3	0	Yes
Nop60B^DL10		Delta2-3	0	--
Nop60B^DL2011	hypomorphic allele - genetic evidence	P-element activity	0	--
Nop60B^DL3		Delta2-3	0	--
Nop60B^DL4		Delta2-3	0	Yes
Nop60B^DL6		Delta2-3	0	--
Nop60B^mM176	hypomorphic allele - genetic evidence	P-element activity	0	--

Alleles Carried on Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Nop60B	Mutagen	Stocks	Known lesion
Nop60B^GD10940	in vitro construct - RNAi in vitro construct - regulatory fusion	2	Yes
Nop60B^GD16822	in vitro construct - RNAi in vitro construct - regulatory fusion	2	Yes
Nop60B^GL00555	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Nop60B^{dsRNA.sp.Scer\UAS}	in vitro construct - regulatory fusion in vitro construct - RNAi	0	Yes
Nop60B^{dsRNA.Sym.Scer\UAS}	in vitro construct - regulatory fusion in vitro construct - RNAi	0	Yes
Nop60B^hs.PG	in vitro construct - regulatory fusion	0	Yes
Nop60B^KK101240	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes

Aneuploid Aberrations

Disrupted in

Df(2R)BSC155

(Christensen and Cook, 2006.5.11)

Df(2R)Exel6081

(Ryder, 2004.4.26)

Df(2R)Exel7184

(Ryder, 2004.4.26)

In(2LR)Px⁴

(Phillips et al., 1998, Giordano et al., 1999, Spradling et al., 1999, Kauffman et al., 2003, BDGP Project Members, 1994-1999)

Not disrupted in

Df(2R)bw^VDe2LPx^KR

(Spradling et al., 1999, Beaton, 1997.12.27)

Df(2R)Px2

(Spradling et al., 1999, Kauffman et al., 2003, BDGP Project Members, 1994-1999)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

heat-shock construct

Insertions

Type of insertions

Name

Expression data

insertion of enhancer trap

P{lacW}l(2)SH0265^SH0265

miscellaneous insertions

PBac{WH}Nop60B^f01318

Gene Ontology: Function, Process & Cellular Component ( 11 unique terms )

Terms Based on Experimental Evidence ( 7 terms )

Molecular Function ( 0 terms)

Biological Process

CV term

Evidence

References

germ cell development