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Gene Dmel\gwl

General Information
Symbol	Dmel\gwl	Species	D. melanogaster
Name	greatwall	Annotation symbol	CG7719
Feature type	protein_coding_gene	FlyBase ID	FBgn0260399
Gene Model Status	Current	Stock availability	6 publicly available
Also Known As	Pk91C, CG7719-related
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	91C5-91C6	Sequence location	3R:14,572,092..14,576,082 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene greatwall is referred to in FlyBase by the symbol Dmel\gwl (CG7719, FBgn0260399). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein serine/threonine kinase activity. There is experimental evidence that it is involved in the biological process: female meiosis; regulation of meiosis; female meiosis I; regulation of mitotic centrosome separation; regulation of mitotic cell cycle; mitosis; regulation of mitosis; chromosome condensation. 29 alleles are reported. The phenotypes of these alleles are annotated with: organ system; organ system subdivision; multicellular structure; organelle; intracellular non-membrane-bounded organelle; intracellular organelle part; sense organ; anatomical structure; centrosome; cell cycle; external compound sense organ. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: AGC-kinase, C-terminal; Protein kinase, catalytic domain; Protein kinase-like domain; Serine/threonine- / dual specificity protein kinase, catalytic domain; Serine/threonine-protein kinase, active site. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1631785_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of gwl. Gene sequence location is 3R:14572092..14576082.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 91C5-91C6   Limits computationally determined from genome sequence between P{PZ}sprd05284 and P{PZ}cdi07013&P{lacW}nosj3B6
Experimentally Determined Cytological Location
Cytogenetic map Notes References 91C-91C   (determined by in situ hybridisation)   (Kalderon and Rubin, 1988)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\gwl for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Low-frequency junction(s) not annotated. Gene model reviewed during 5.44
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) gwl-RA FBtr0083674  NM_080121   3099   846 gwl-RB FBtr0331352  NM_001275784   2970   803
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein gwl-PA   FBpp0083089   93.5   846   6.74     NP_524860   AAF55594 gwl-PB   FBpp0303770   88.7   803   6.88     NP_001262713   AGB96093
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, catalytic domain (IPR000719) AGC-kinase, C-terminal (IPR000961) Serine/threonine- / dual specificity protein kinase, catalytic domain (IPR002290) Serine/threonine-protein kinase, active site (IPR008271) Protein kinase-like domain (IPR011009)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA201414   AA202438   AA390488   AA439782   AA816651   AA978536   AE014297 AAF55594   AE014297 AGB96093   AI455607   AQ025101   AW943260   AY058648 AAL13877   BF505638   BI234812   BI235351   BI237326   BI482659   BI483294   CO177210   CO185703   CO269041   CO270201   CO300595   CO303838   CO321450   CO326555   CO345235   CQ847768   CZ487259   CZ487260   EC051021   EC052490   EC053985   EC054482   EC056192   EC056311   EC056459   EC057110   EC062955   EC069609   EC075739   EC082566   EC090456   EC212387   EC236868   EC246083   EC252694   EV590854
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q95TN8
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4   organism   • yolk plasma | posterior (Lecuyer et al., 2007) embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) pole cell   (Fisher et al., 2012) pole plasm   (Fisher et al., 2012) embryonic stage 5   pole cell   (Lecuyer et al., 2007) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) pole cell   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   anterior midgut primordium   (Fisher et al., 2012) central brain primordium   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) posterior midgut primordium   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic neuroblast of embryonic central brain   (Fisher et al., 2012) embryonic/larval nervous system   (Fisher et al., 2012) neuroblast of ventral nerve cord primordium   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    65.375 Larval Midgut    3.3 Larval Hindgut    3.2 Larval Malpighian Tubules    5.1 Larval Fat Body    24.5 Larval Salivary Gland    10.9 Larval Trachea    15.4 Larval Carcass    9.1 Adult Head    2.5 Adult Eye    4 Adult Brain    0.9 Adult Thoracic-Abdominal Ganglion    2.4 Adult Crop    5.2 Adult Midgut    3.2 Adult Hindgut    2.2 Adult Malpighian Tubules    6.9 Adult Fat Body    5.6 Adult Salivary Gland    7.7 Adult Heart    0.475 Adult VirginFemale Spermatheca    8.1 Adult InseminatedFemale Spermatheca    5.1 Adult Ovary    605.5 Adult Testis    95.2 Adult Male Accessory Gland    4 Adult Carcass    14.4 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    65.375 Larval Midgut    3.3 Larval Hindgut    3.2 Larval Malpighian Tubules    5.1 Larval Fat Body    24.5 Larval Salivary Gland    10.9 Larval Trachea    15.4 Larval Carcass    9.1 Adult Head    2.5 Adult Eye    4 Adult Brain    0.9 Adult Thoracic-Abdominal Ganglion    2.4 Adult Crop    5.2 Adult Midgut    3.2 Adult Hindgut    2.2 Adult Malpighian Tubules    6.9 Adult Fat Body    5.6 Adult Salivary Gland    7.7 Adult Heart    0.475 Adult VirginFemale Spermatheca    8.1 Adult InseminatedFemale Spermatheca    5.1 Adult Ovary  (605.5) Adult Testis    95.2 Adult Male Accessory Gland    4 Adult Carcass    14.4 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    65.375 Larval Midgut    3.3 Larval Hindgut    3.2 Larval Malpighian Tubules    5.1 Larval Fat Body    24.5 Larval Salivary Gland    10.9 Larval Trachea    15.4 Larval Carcass    9.1 Adult Head    2.5 Adult Eye    4 Adult Brain    0.9 Adult Thoracic-Abdominal Ganglion    2.4 Adult Crop    5.2 Adult Midgut    3.2 Adult Hindgut    2.2 Adult Malpighian Tubules    6.9 Adult Fat Body    5.6 Adult Salivary Gland    7.7 Adult Heart    0.475 Adult VirginFemale Spermatheca    8.1 Adult InseminatedFemale Spermatheca    5.1 Adult Ovary    605.5 Adult Testis    95.2 Adult Male Accessory Gland    4 Adult Carcass    14.4 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    65.375 Larval Midgut    3.3 Larval Hindgut    3.2 Larval Malpighian Tubules    5.1 Larval Fat Body    24.5 Larval Salivary Gland    10.9 Larval Trachea    15.4 Larval Carcass    9.1 Adult Head    2.5 Adult Eye    4 Adult Brain    0.9 Adult Thoracic-Abdominal Ganglion    2.4 Adult Crop    5.2 Adult Midgut    3.2 Adult Hindgut    2.2 Adult Malpighian Tubules    6.9 Adult Fat Body    5.6 Adult Salivary Gland    7.7 Adult Heart    0.475 Adult VirginFemale Spermatheca    8.1 Adult InseminatedFemale Spermatheca    5.1 Adult Ovary    605.5 Adult Testis    95.2 Adult Male Accessory Gland    4 Adult Carcass    14.4 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    65.375 Larval Midgut    3.3 Larval Hindgut    3.2 Larval Malpighian Tubules    5.1 Larval Fat Body    24.5 Larval Salivary Gland    10.9 Larval Trachea    15.4 Larval Carcass    9.1 Adult Head    2.5 Adult Eye    4 Adult Brain    0.9 Adult Thoracic-Abdominal Ganglion    2.4 Adult Crop    5.2 Adult Midgut    3.2 Adult Hindgut    2.2 Adult Malpighian Tubules    6.9 Adult Fat Body    5.6 Adult Salivary Gland    7.7 Adult Heart    0.475 Adult VirginFemale Spermatheca    8.1 Adult InseminatedFemale Spermatheca    5.1 Adult Ovary    605.5 Adult Testis    95.2 Adult Male Accessory Gland    4 Adult Carcass    14.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    65.375 Larval Midgut    3.3 Larval Hindgut    3.2 Larval Malpighian Tubules    5.1 Larval Fat Body    24.5 Larval Salivary Gland    10.9 Larval Trachea    15.4 Larval Carcass    9.1 Adult Head    2.5 Adult Eye    4 Adult Brain    0.9 Adult Thoracic-Abdominal Ganglion    2.4 Adult Crop    5.2 Adult Midgut    3.2 Adult Hindgut    2.2 Adult Malpighian Tubules    6.9 Adult Fat Body    5.6 Adult Salivary Gland    7.7 Adult Heart    0.475 Adult VirginFemale Spermatheca    8.1 Adult InseminatedFemale Spermatheca    5.1 Adult Ovary  605.5 Adult Testis    95.2 Adult Male Accessory Gland    4 Adult Carcass    14.4 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    65.375 Larval Midgut    3.3 Larval Hindgut    3.2 Larval Malpighian Tubules    5.1 Larval Fat Body    24.5 Larval Salivary Gland    10.9 Larval Trachea    15.4 Larval Carcass    9.1 Adult Head    2.5 Adult Eye    4 Adult Brain    0.9 Adult Thoracic-Abdominal Ganglion    2.4 Adult Crop    5.2 Adult Midgut    3.2 Adult Hindgut    2.2 Adult Malpighian Tubules    6.9 Adult Fat Body    5.6 Adult Salivary Gland    7.7 Adult Heart    0.475 Adult VirginFemale Spermatheca    8.1 Adult InseminatedFemale Spermatheca    5.1 Adult Ovary    605.5 Adult Testis    95.2 Adult Male Accessory Gland    4 Adult Carcass    14.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    65.375 Larval Midgut    3.3 Larval Hindgut    3.2 Larval Malpighian Tubules    5.1 Larval Fat Body    24.5 Larval Salivary Gland    10.9 Larval Trachea    15.4 Larval Carcass    9.1 Adult Head    2.5 Adult Eye    4 Adult Brain    0.9 Adult Thoracic-Abdominal Ganglion    2.4 Adult Crop    5.2 Adult Midgut    3.2 Adult Hindgut    2.2 Adult Malpighian Tubules    6.9 Adult Fat Body    5.6 Adult Salivary Gland    7.7 Adult Heart    0.475 Adult VirginFemale Spermatheca    8.1 Adult InseminatedFemale Spermatheca    5.1 Adult Ovary    605.5 Adult Testis    95.2 Adult Male Accessory Gland    4 Adult Carcass    14.4 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    2.5   NA  Eye    4   NA  Brain    0.9   65.375  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    2.4   NA  Crop    5.2   3.3  Midgut    3.2   3.2  Hindgut    2.2   5.1  Malpighian Tubules    6.9   no informative data  Fat Body    5.6   no informative data  Salivary Gland    7.7   NA  Heart    0.475   15.4  Trachea    NA   NA  VirginFemale Spermatheca    8.1   NA  InseminatedFemale Spermatheca    5.1   NA  Ovary    605.5   NA  Testis    95.2   NA  Male Accessory Gland    4   9.1  Carcass    no informative data FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    5 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    1 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    1 digestive system, 4-day adult    4 digestive system, 20-day adult    3 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    0 carcass, larvae L3 wandering    5 carcass, 1-day adult    2 carcass, 4-day adult    6 carcass, 20-day adult    4 ovary, virgin 4-day female    147 ovary, mated 4-day female    118 testis, mated 4-day male    14 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    5 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    1 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    1 digestive system, 4-day adult    4 digestive system, 20-day adult    3 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    0 carcass, larvae L3 wandering    5 carcass, 1-day adult    2 carcass, 4-day adult    6 carcass, 20-day adult    4 ovary, virgin 4-day female  (147) ovary, mated 4-day female  (118) testis, mated 4-day male    14 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    5 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    1 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    1 digestive system, 4-day adult    4 digestive system, 20-day adult    3 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    0 carcass, larvae L3 wandering    5 carcass, 1-day adult    2 carcass, 4-day adult    6 carcass, 20-day adult    4 ovary, virgin 4-day female  (147) ovary, mated 4-day female  (118) testis, mated 4-day male    14 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    5 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    1 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    1 digestive system, 4-day adult    4 digestive system, 20-day adult    3 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    0 carcass, larvae L3 wandering    5 carcass, 1-day adult    2 carcass, 4-day adult    6 carcass, 20-day adult    4 ovary, virgin 4-day female    147 ovary, mated 4-day female    118 testis, mated 4-day male    14 accessory gland, mated 4-day male    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    5 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    1 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    1 digestive system, 4-day adult    4 digestive system, 20-day adult    3 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    0 carcass, larvae L3 wandering    5 carcass, 1-day adult    2 carcass, 4-day adult    6 carcass, 20-day adult    4 ovary, virgin 4-day female    147 ovary, mated 4-day female    118 testis, mated 4-day male    14 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    5 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    1 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    1 digestive system, 4-day adult    4 digestive system, 20-day adult    3 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    0 carcass, larvae L3 wandering    5 carcass, 1-day adult    2 carcass, 4-day adult    6 carcass, 20-day adult    4 ovary, virgin 4-day female  (147) ovary, mated 4-day female  (118) testis, mated 4-day male    14 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    5 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    1 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    1 digestive system, 4-day adult    4 digestive system, 20-day adult    3 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    0 carcass, larvae L3 wandering    5 carcass, 1-day adult    2 carcass, 4-day adult    6 carcass, 20-day adult    4 ovary, virgin 4-day female  (147) ovary, mated 4-day female  118 testis, mated 4-day male    14 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    5 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    1 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    1 digestive system, 4-day adult    4 digestive system, 20-day adult    3 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    0 carcass, larvae L3 wandering    5 carcass, 1-day adult    2 carcass, 4-day adult    6 carcass, 20-day adult    4 ovary, virgin 4-day female    147 ovary, mated 4-day female    118 testis, mated 4-day male    14 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    155 embryo 02-04hr    67 embryo 04-06hr    56 embryo 06-08hr    41 embryo 08-10hr    52 embryo 10-12hr    28 embryo 12-14hr    23 embryo 14-16hr    30 embryo 16-18hr    26 embryo 18-20hr    10 embryo 20-22hr    36 embryo 22-24hr    38 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    6 white prepupae new    7 white prepupae 12hr    9 white prepupae 24hr    8 pupae 2d postWPP    5 pupae 3d postWPP    2 pupae 4d postWPP    3 adult male 01day    3 adult male 05day    5 adult male 30day    4 adult female 01day    51 adult female 05day    102 adult female 30day    103 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (155) embryo 02-04hr  (67) embryo 04-06hr  (56) embryo 06-08hr  (41) embryo 08-10hr  (52) embryo 10-12hr  (28) embryo 12-14hr    23 embryo 14-16hr  (30) embryo 16-18hr  26 embryo 18-20hr    10 embryo 20-22hr  (36) embryo 22-24hr  (38) larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    6 white prepupae new    7 white prepupae 12hr    9 white prepupae 24hr    8 pupae 2d postWPP    5 pupae 3d postWPP    2 pupae 4d postWPP    3 adult male 01day    3 adult male 05day    5 adult male 30day    4 adult female 01day  (51) adult female 05day  (102) adult female 30day  (103) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (155) embryo 02-04hr    67 embryo 04-06hr    56 embryo 06-08hr    41 embryo 08-10hr    52 embryo 10-12hr    28 embryo 12-14hr    23 embryo 14-16hr    30 embryo 16-18hr    26 embryo 18-20hr    10 embryo 20-22hr    36 embryo 22-24hr    38 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    6 white prepupae new    7 white prepupae 12hr    9 white prepupae 24hr    8 pupae 2d postWPP    5 pupae 3d postWPP    2 pupae 4d postWPP    3 adult male 01day    3 adult male 05day    5 adult male 30day    4 adult female 01day    51 adult female 05day  102 adult female 30day  103 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    155 embryo 02-04hr    67 embryo 04-06hr    56 embryo 06-08hr    41 embryo 08-10hr    52 embryo 10-12hr    28 embryo 12-14hr    23 embryo 14-16hr    30 embryo 16-18hr    26 embryo 18-20hr    10 embryo 20-22hr    36 embryo 22-24hr    38 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    6 white prepupae new    7 white prepupae 12hr    9 white prepupae 24hr    8 pupae 2d postWPP    5 pupae 3d postWPP    2 pupae 4d postWPP    3 adult male 01day    3 adult male 05day    5 adult male 30day    4 adult female 01day    51 adult female 05day    102 adult female 30day    103 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    155 embryo 02-04hr    67 embryo 04-06hr    56 embryo 06-08hr    41 embryo 08-10hr    52 embryo 10-12hr    28 embryo 12-14hr    23 embryo 14-16hr    30 embryo 16-18hr    26 embryo 18-20hr    10 embryo 20-22hr    36 embryo 22-24hr    38 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    6 white prepupae new    7 white prepupae 12hr    9 white prepupae 24hr    8 pupae 2d postWPP    5 pupae 3d postWPP    2 pupae 4d postWPP    3 adult male 01day    3 adult male 05day    5 adult male 30day    4 adult female 01day    51 adult female 05day    102 adult female 30day    103 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (155) embryo 02-04hr  (67) embryo 04-06hr  (56) embryo 06-08hr  (41) embryo 08-10hr  (52) embryo 10-12hr  28 embryo 12-14hr    23 embryo 14-16hr  30 embryo 16-18hr  26 embryo 18-20hr    10 embryo 20-22hr  (36) embryo 22-24hr  (38) larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    6 white prepupae new    7 white prepupae 12hr    9 white prepupae 24hr    8 pupae 2d postWPP    5 pupae 3d postWPP    2 pupae 4d postWPP    3 adult male 01day    3 adult male 05day    5 adult male 30day    4 adult female 01day  (51) adult female 05day  (102) adult female 30day  (103) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (155) embryo 02-04hr    67 embryo 04-06hr    56 embryo 06-08hr    41 embryo 08-10hr    52 embryo 10-12hr    28 embryo 12-14hr    23 embryo 14-16hr    30 embryo 16-18hr    26 embryo 18-20hr    10 embryo 20-22hr    36 embryo 22-24hr    38 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    6 white prepupae new    7 white prepupae 12hr    9 white prepupae 24hr    8 pupae 2d postWPP    5 pupae 3d postWPP    2 pupae 4d postWPP    3 adult male 01day    3 adult male 05day    5 adult male 30day    4 adult female 01day    51 adult female 05day  102 adult female 30day  103 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    155 embryo 02-04hr    67 embryo 04-06hr    56 embryo 06-08hr    41 embryo 08-10hr    52 embryo 10-12hr    28 embryo 12-14hr    23 embryo 14-16hr    30 embryo 16-18hr    26 embryo 18-20hr    10 embryo 20-22hr    36 embryo 22-24hr    38 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    6 white prepupae new    7 white prepupae 12hr    9 white prepupae 24hr    8 pupae 2d postWPP    5 pupae 3d postWPP    2 pupae 4d postWPP    3 adult male 01day    3 adult male 05day    5 adult male 30day    4 adult female 01day    51 adult female 05day    102 adult female 30day    103 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    19 Schneider line 2 Sg4    11 embryonic 1182-4H    18 embryonic GM2    9 embryonic Kc167    11 embryonic S1    10 embryonic S3    12 leg disc CME L1    9 wing disc CME-W2    11 wing disc ML-DmD8    12 wing disc ML-DmD9    9 wing disc ML-DmD16-c3    9 wing disc ML-DmD21    8 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    5 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    9 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    3 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    12 ovary fGS/OSS    9 ovary OSC    9 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    19 Schneider line 2 Sg4    11 embryonic 1182-4H    18 embryonic GM2    9 embryonic Kc167    11 embryonic S1    10 embryonic S3    12 leg disc CME L1    9 wing disc CME-W2    11 wing disc ML-DmD8    12 wing disc ML-DmD9    9 wing disc ML-DmD16-c3    9 wing disc ML-DmD21    8 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    5 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    9 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    3 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    12 ovary fGS/OSS    9 ovary OSC    9 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    19 Schneider line 2 Sg4    11 embryonic 1182-4H    18 embryonic GM2    9 embryonic Kc167    11 embryonic S1    10 embryonic S3    12 leg disc CME L1    9 wing disc CME-W2    11 wing disc ML-DmD8    12 wing disc ML-DmD9    9 wing disc ML-DmD16-c3    9 wing disc ML-DmD21    8 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    5 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    9 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    3 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    12 ovary fGS/OSS    9 ovary OSC    9 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    19 Schneider line 2 Sg4    11 embryonic 1182-4H    18 embryonic GM2    9 embryonic Kc167    11 embryonic S1    10 embryonic S3    12 leg disc CME L1    9 wing disc CME-W2    11 wing disc ML-DmD8    12 wing disc ML-DmD9    9 wing disc ML-DmD16-c3    9 wing disc ML-DmD21    8 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    5 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    9 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    3 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    12 ovary fGS/OSS    9 ovary OSC    9 ovary OSS    5 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    19 Schneider line 2 Sg4    11 embryonic 1182-4H    18 embryonic GM2    9 embryonic Kc167    11 embryonic S1    10 embryonic S3    12 leg disc CME L1    9 wing disc CME-W2    11 wing disc ML-DmD8    12 wing disc ML-DmD9    9 wing disc ML-DmD16-c3    9 wing disc ML-DmD21    8 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    5 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    9 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    3 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    12 ovary fGS/OSS    9 ovary OSC    9 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    19 Schneider line 2 Sg4    11 embryonic 1182-4H    18 embryonic GM2    9 embryonic Kc167    11 embryonic S1    10 embryonic S3    12 leg disc CME L1    9 wing disc CME-W2    11 wing disc ML-DmD8    12 wing disc ML-DmD9    9 wing disc ML-DmD16-c3    9 wing disc ML-DmD21    8 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    5 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    9 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    3 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    12 ovary fGS/OSS    9 ovary OSC    9 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    19 Schneider line 2 Sg4    11 embryonic 1182-4H    18 embryonic GM2    9 embryonic Kc167    11 embryonic S1    10 embryonic S3    12 leg disc CME L1    9 wing disc CME-W2    11 wing disc ML-DmD8    12 wing disc ML-DmD9    9 wing disc ML-DmD16-c3    9 wing disc ML-DmD21    8 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    5 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    9 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    3 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    12 ovary fGS/OSS    9 ovary OSC    9 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    19 Schneider line 2 Sg4    11 embryonic 1182-4H    18 embryonic GM2    9 embryonic Kc167    11 embryonic S1    10 embryonic S3    12 leg disc CME L1    9 wing disc CME-W2    11 wing disc ML-DmD8    12 wing disc ML-DmD9    9 wing disc ML-DmD16-c3    9 wing disc ML-DmD21    8 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    5 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    9 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    3 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    12 ovary fGS/OSS    9 ovary OSC    9 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    11 heat shock, 4-day adult    31 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    24 Cadmium 100 mM 48 hrs, 4-day adult    76 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    32 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    15 Ethanol 2.5% 3 hrs, larvae L3    93 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    69 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    55 Paraquat 10 mM 48 hrs, 4-day adult    51 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    11 heat shock, 4-day adult  (31) Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    24 Cadmium 100 mM 48 hrs, 4-day adult  (76) Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult  (32) Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    15 Ethanol 2.5% 3 hrs, larvae L3  (93) Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (69) Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult  (55) Paraquat 10 mM 48 hrs, 4-day adult  (51) Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    11 heat shock, 4-day adult    31 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    24 Cadmium 100 mM 48 hrs, 4-day adult    76 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    32 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    15 Ethanol 2.5% 3 hrs, larvae L3    93 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    69 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    55 Paraquat 10 mM 48 hrs, 4-day adult    51 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    11 heat shock, 4-day adult    31 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    24 Cadmium 100 mM 48 hrs, 4-day adult    76 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    32 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    15 Ethanol 2.5% 3 hrs, larvae L3    93 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    69 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    55 Paraquat 10 mM 48 hrs, 4-day adult    51 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    11 heat shock, 4-day adult    31 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    24 Cadmium 100 mM 48 hrs, 4-day adult    76 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    32 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    15 Ethanol 2.5% 3 hrs, larvae L3    93 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    69 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    55 Paraquat 10 mM 48 hrs, 4-day adult    51 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    11 heat shock, 4-day adult  31 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    24 Cadmium 100 mM 48 hrs, 4-day adult  (76) Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult  32 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    15 Ethanol 2.5% 3 hrs, larvae L3  (93) Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (69) Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult  (55) Paraquat 10 mM 48 hrs, 4-day adult  (51) Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    11 heat shock, 4-day adult    31 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    24 Cadmium 100 mM 48 hrs, 4-day adult    76 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    32 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    15 Ethanol 2.5% 3 hrs, larvae L3    93 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    69 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    55 Paraquat 10 mM 48 hrs, 4-day adult    51 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    11 heat shock, 4-day adult    31 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    24 Cadmium 100 mM 48 hrs, 4-day adult    76 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    32 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    15 Ethanol 2.5% 3 hrs, larvae L3    93 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    69 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    55 Paraquat 10 mM 48 hrs, 4-day adult    51 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_01 mE1_20_mRNA_expression_cluster_04
External Data & Images
Linkouts	BDGP expression data • LD35132 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0260399
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal gwl716 gwlGD9947 lethal | larval stage gwl180 partially lethal - majority die gwl2 gwl3a gwl6a partially lethal - majority live (with gwl6a) gwlSr3 partially lethal - majority live (with gwlSr3) gwl6a gwlSr6 partially lethal - majority live (with gwlSr6) gwlSr3 viable gwlscant viable, with Scer\GAL4pnr-MD237 gwlGD9947 viable (with Df(3L)gwl3b) gwlSr18 Sterility Allele female fertile (with gwl2) gwlSr3 gwlSr6 female fertile (with gwl3a) gwlSr3 gwlSr6 female fertile (with gwl6a) gwlSr3 gwlSr6 female fertile (with gwlSr3) gwl2 gwl3a gwl6a female fertile (with gwlSr6) gwl2 gwl3a gwl6a female sterile gwlscant female sterile (with Df(3L)gwl3b) gwlSr18 fertile (with gwl2) gwlSr6 fertile (with gwl3a) gwlSr6 fertile (with gwl6a) gwlSr6 fertile (with gwlSr6) gwl2 gwl3a gwl6a sterile gwl6a gwlSr3 gwlSr6 sterile (with gwl6a) gwlSr3 sterile (with gwlSr3) gwl6a Other Phenotypes Allele eclosion rhythm defective gwlSr3 eclosion rhythm defective (with gwl6a) gwlSr3 eclosion rhythm defective (with gwlSr3) gwl6a gwlSr6 eclosion rhythm defective (with gwlSr6) gwlSr3 meiotic cell cycle defective (with Df(3L)gwl3b) gwlSr18 meiotic cell cycle defective | oogenesis (with gwl180) gwlSr18 meiotic cell cycle defective | oogenesis (with gwlSr18) gwl180 mitotic cell cycle defective gwl716 gwldsRNA.cYa gwlunspecified mitotic cell cycle defective (with Df(3L)gwl3b) gwl6a mitotic cell cycle defective (with gwl6a) gwlSr3 mitotic cell cycle defective (with gwlSr3) gwl6a gwlSr6 mitotic cell cycle defective (with gwlSr6) gwlSr3 mitotic cell cycle defective | larval stage gwl716 mitotic cell cycle defective | larval stage (with gwl180) gwl716 mitotic cell cycle defective | larval stage (with gwl716) gwl180 neuroanatomy defective gwldsRNA.cIa neuroanatomy defective | larval stage, with Scer\GAL4elav-C155 gwlEP515 Phenotype manifest in Allele anterior fascicle & synapse, with Scer\GAL4elav-C155 gwlEP515 anterior fascicle & synapse | supernumerary, with Scer\GAL4elav-C155 gwlEP515 centrosome (with Df(3L)gwl3b) gwlSr18 chromatin gwlSr6 chromatin (with Df(3L)gwl3b) gwl6a chromatin (with gwl6a) gwlSr3 chromatin (with gwlSr3) gwlSr6 gwl6a chromatin (with gwlSr6) gwlSr3 chromosome gwldsRNA.cYa condensed chromosome gwl716 gwlunspecified egg (with Df(3L)gwl3b) gwlSr18 embryonic/larval brain gwlscant embryonic/larval brain | larval stage gwlGD9947 embryonic/larval neuroblast | larval stage gwl716 embryonic/larval neuroblast | larval stage (with gwl180) gwl716 embryonic/larval neuroblast | larval stage (with gwl716) gwl180 eye gwlSr3 eye (with gwl6a) gwlSr3 eye (with gwlSr3) gwlSr6 gwl6a eye (with gwlSr6) gwlSr3 gonad gwlSr3 gonad (with gwl6a) gwlSr3 gonad (with gwlSr3) gwlSr6 gwl6a gonad (with gwlSr6) gwlSr3 intersegmental nerve, with Scer\GAL4elav-C155 gwlEP515 longitudinal connective gwldsRNA.cIa macrochaeta gwlSr3 macrochaeta (with gwl6a) gwlSr3 macrochaeta (with gwlSr3) gwlSr6 gwl6a macrochaeta (with gwlSr6) gwlSr3 mitotic cell cycle gwldsRNA.cYa mitotic cell cycle | third instar larval stage gwl716 gwlunspecified neuroblast & mitotic cell cycle gwl716 gwlunspecified oocyte (with Df(3L)gwl3b) gwlSr18 segmental nerve | embryonic stage gwldsRNA.cIa spindle (with Df(3L)gwl3b) gwlSr18 ventral nerve cord commissure gwldsRNA.cIa wing margin gwlSr3 wing margin (with gwl6a) gwlSr3 wing margin (with gwlSr3) gwlSr6 gwl6a wing margin (with gwlSr6) gwlSr3
Classical Alleles ( 18 )
For All Classical Alleles Show
Allele of gwl Class Mutagen Stocks Known lesion gwlEP515 P-element activity 1 Yes gwlf04701 piggyBac transposase 1 -- gwlMB01301 1 -- gwl1028 ethyl methanesulfonate 0 Yes gwl1080 0 -- gwl180 0 Yes gwl2790 0 -- gwl2970 0 Yes gwl2 P-element activity 0 Yes gwl3a P-element activity 0 Yes gwl6a loss of function allele P-element activity 0 Yes gwl716 0 Yes gwl873 0 Yes gwlscant gain of function allele ethyl methanesulfonate 0 Yes gwlSr18 hypomorphic allele - genetic evidence 0 Yes gwlSr3 hypomorphic allele - genetic evidence X ray 0 Yes gwlSr6 hypomorphic allele - genetic evidence X ray 0 Yes gwlunspecified 0 --
Alleles Carried on Transgenic Constructs ( 11 )
For All Alleles Carried on Transgenic Constructs Show
Allele of gwl Class Mutagen Stocks Known lesion gwlGD9947 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes gwlGL00091 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes gwlHMS00834 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes gwldsRNA.cIa in vitro construct - RNAi 0 Yes gwldsRNA.cYa in vitro construct - RNAi 0 Yes gwlK87R.Scer\UAS.P\T.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - site directed mutagenesis 0 Yes gwlK97M.Scer\UAS in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes gwlL.Scer\UAS in vitro construct - regulatory fusion 0 Yes gwlNIG.7719R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes gwlS.Scer\UAS in vitro construct - regulatory fusion 0 Yes gwlScer\UAS.P\T.T:Hsap\MYC in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3L)gwl3b (Archambault et al., 2007) Df(3R)Exel6181 (Ryder, 2004.4.26, Archambault et al., 2007)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD9947} NA P{NIG.7719R} NA P{TRiP.GL00091} NA P{TRiP.HMS00834} NA P{UAS-gwl-short} NA P{UASp-gwl.K97M} NA P{UASp-gwl.Long} NA P{UASp-gwl.MYC.K87R} NA P{UASp-gwl.MYC} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}gwlEP515 NA miscellaneous insertions PBac{WH}gwlf04701 NA insertion of enhancer trap binary system Mi{ET1}gwlMB01301 No
Gene Ontology: Function, Process & Cellular Component ( 12 unique terms )
Terms Based on Experimental Evidence ( 9 terms )
Molecular Function
CV term Evidence References
protein serine/threonine kinase activity inferred from direct assay (Kim et al., 2012)
Biological Process
CV term Evidence References
chromosome condensation inferred from mutant phenotype (Yu et al., 2004) female meiosis inferred from mutant phenotype (Kim et al., 2012) female meiosis I inferred from mutant phenotype (Archambault et al., 2007) mitosis inferred from mutant phenotype (Yu et al., 2002) regulation of meiosis inferred from mutant phenotype (Archambault et al., 2007) regulation of mitosis inferred from mutant phenotype (Archambault et al., 2007, Kim et al., 2012) regulation of mitotic cell cycle inferred from mutant phenotype (Yu et al., 2004) regulation of mitotic centrosome separation inferred from mutant phenotype (Archambault et al., 2007)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR000961, InterPro:IPR002290 (FlyBase Curators et al., 2004-) protein kinase activity non-traceable author statement (Morrison et al., 2000) protein serine/threonine kinase activity inferred from sequence or structural similarity (Kalderon and Rubin, 1988)
Biological Process
CV term Evidence References
protein phosphorylation inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR000961, InterPro:IPR002290, InterPro:IPR008271 (FlyBase Curators et al., 2004-) inferred from sequence or structural similarity (Kalderon and Rubin, 1988) non-traceable author statement (Morrison et al., 2000)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	gene   nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions gwl allele Gene References unspecified polo (Glover, 1995)
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0260399 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 45969 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q95TN8
Orthologs
OrthoDB Orthologs (38) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6V9V94 Dipteran inclusive ortholog search EOG6Q58XH Insect inclusive ortholog search EOG6VX116 Arthropod inclusive ortholog search EOG61NS22 Metazoa inclusive ortholog search EOG62NWS3
Orthologs in Drosophila Species (EOG6V9V94)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\gwl     Drosophila simulans   Dsim\GD19248     Drosophila sechellia   Dsec\GM17889 Y   Drosophila erecta   Dere\GG23066 Y   Drosophila yakuba   Dyak\GE25560 Y   Drosophila ananassae   Dana\GF23215 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA20542 Y   Drosophila persimilis   Dper\GL23525 Y   Drosophila willistoni   Dwil\GK22740 Y   Drosophila virilis   Dvir\GJ14542 Y   Drosophila mojavensis   Dmoj\GI22962 Y   Drosophila grimshawi   Dgri\GH14461 Y
Orthologs in non-Drosophila Dipterans (EOG6Q58XH)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL002569   Anopheles gambiae Malaria mosquito  Agam\AGAP001636   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ002365
Orthologs in non-Dipteran Insects (EOG6VX116)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\LOC411080   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG003049   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH10904   Atta cephalotes Leafcutter ant  Acep\ACEP20147   Camponotus floridanus Florida carpenter ant  Cflo\CFLO22459   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL19575   Linepithema humile Argentine ant  Lhum\LH18597   Pogonomyrmex barbatus Red harvester ant  Pbar\PB14888   Solenopsis invicta Red fire ant  Sinv\SINV20217   Acyrthosiphon pisum Pea aphid  Apim\ACYPI004551   Bombyx mori Silkmoth  Bmor\BGIBMGA003733   Bombyx mori Silkmoth  Bmor\BGIBMGA003734   Pediculus humanus Human body louse  Phum\PHUM380500   Tribolium castaneum Red flour beetle  Tcas\TC003692
Orthologs in non-Insect Arthropods (EOG61NS22)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_328306   Ixodes scapularis Deer tick  Isca\ISCW015651
Orthologs in non-Arthropod Metazoa (EOG62NWS3)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_011838gn   Danio rerio Zebrafish  Drer\mastl   Xenopus tropicalis Western clawed frog  Xtro\mastl   Gallus gallus Domestic chicken  Ggal\MASTL   Mus musculus House mouse  Mmus\Mastl   Rattus norvegicus Norway rat  Rnor\Mastl   Homo sapiens Human  Hsap\ENSG00000120539
Human Orthologs (1)
Gene OMIM HGNC Hsap\ENSG00000120539
AAA Orthologs (10) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila sechellia Dsec\GM17889 Drosophila erecta Dere\GG23066 Drosophila yakuba Dyak\GE25560 Drosophila ananassae Dana\GF23215 Drosophila pseudoobscura pseudoobscura Dpse\GA20542 Drosophila persimilis Dper\GL23525 Drosophila willistoni Dwil\GK22740 Drosophila virilis Dvir\GJ14542 Drosophila mojavensis Dmoj\GI22962 Drosophila grimshawi Dgri\GH14461
Stocks & Reagents
Stocks Listed in FlyBase ( 6 )
Bloomington	23052 y1 w67c23; Mi{ET1}gwlMB01301 17174 w1118; P{EP}gwlEP515/TM6B, Tb1 35212 y1 sc* v1; P{TRiP.GL00091}attP2 34525 y1 sc* v1; P{TRiP.HMS00834}attP2
Harvard	- PBac{WH}gwlf04701
VDRC	v21046 w1118; P{GD9947}v21046
Genomic Clones ( 2 )
	BACR01F15 BACR40J10 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 40 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD35132
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT07907 LD02624 LD03131 LD04412 LD09348 LD14440 LD30784 RE30570 RE31186 RE33539 RE65376 RE66166
Other clones	57754 60911 70934 80992 103235 194871 214251.58 CK01.8A.H7 CK01.67A.H8 CK01.122C.F11 Dmel_neb_000041_0350_3756 EC051021 EC052490 EC053985 EC054482 EC056192 EC056311 EC056459 EK010932 EK052764 EK093424 EK097907 EK178651 EK185717 EK242922 EP04205 EP21651
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0260399 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 45969
Antibody Information
	monoclonal (Archambault et al., 2007)
Other Information
Discoverer
Etymology
	The gene is named "greatwall" because the mutant phenotype suggests a role in protecting chromosome structure. (Yu et al., 2004)
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: gwl Pk91C (Yu, 2002.3.29)
Source for database merge of	Source for merge of: gwl scant (Archambault et al., 2007) Source for merge of: Pk91C CG7719 (Bayraktaroglu, 2000.11.2)
Additional comments
Other Comments
	gwl enhances mitotic defects resulting from a decrease in polo function. (Archambault et al., 2007) gwl is required for sister chromatid cohesion in female meiosis I. (Archambault et al., 2007) gwl has multiple roles in progression through mitosis and meiosis. (Archambault et al., 2007) When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an increase in the proportion of G2/M phase cells, an increase in mitotic index, a decrease in the ratio of cells in prometaphase and metaphase versus the total number of mitotic cells, a whole range of mitotic abnormalities, spindle abnormalities, chromosome abnormalities, chromosome segregation defects are seen. (Bettencourt-Dias et al., 2004) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) Mutants exhibit a high mitotic index and metaphase arrest. (Yu et al., 2002) Five genes essential for cell cycle progression, polo, mgr, asp, stg and gnu, are recombined onto a single chromosome, EMS mutations are isolated that fail to complement this chromosome. Genes encoding proteins that interact with the products of any of the five genes are isolated. (White-Cooper et al., 1996) Embryos from mothers transheterozygous for mutations in polo and scant show bipolar spindles that have a centrosome only at one pole. (Glover, 1995) Isolated from a genomic library using a Pka-C1 probe. (Kalderon and Rubin, 1988) gwl may encode a protein kinase. (Kalderon and Rubin, 1988) Gene in D.melanogaster encoding product related (by sequence comparison) to the serine-threonine protein kinases of mammals. Isolated from Drosophila clones obtained with mammalian probes.
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_080121 NM_001275784 RefSeq (Proteins) • NP_524860 NP_001262713 DDBJ / EMBL / GenBank • AAF55594 AAL13877 AGB96093 AI455607 AQ025101 AW943260 AY058648 CQ847768 CZ487259 CZ487260 Entrez Gene - A searchable database of RefSeq genes. • 45969 UniProtKB/TrEMBL • Q95TN8
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, catalytic domain (IPR000719) AGC-kinase, C-terminal (IPR000961) Serine/threonine- / dual specificity protein kinase, catalytic domain (IPR002290) Serine/threonine-protein kinase, active site (IPR008271) Protein kinase-like domain (IPR011009) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD35132
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0260399 DRSC - Results from RNAi screens. • FBgn0260399 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0260399 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0260399 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 45969 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /genebrief/greatwall.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 45969 modMine - Data generated by the modENCODE project. • FBgn0260399 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q95TN8
Synonyms & Secondary IDs ( 13 )
Reported As
Symbol Synonym	1J (Kalderon and Rubin, 1988, ) anon-WO2004063362.73   CG7719 (Yu et al., 2004, Asha et al., 2003, Yu, 2002.9.17, Ni et al., 2010.12.1, Ni et al., 2011.7.19) CG7719-related (Kraut et al., 2001, Kraut et al., 2001) CT23435 (Morrison et al., 2000) EP0515 (Kraut et al., 2001) gwl (Archambault et al., 2007, Lecuyer et al., 2007, Somma et al., 2008, Kim et al., 2012, Mattila et al., 2008, Wang et al., 2011, Guenin et al., 2010, Japanese National Institute of Genetics, 2012.5.21, Soshnev et al., 2012) Pk91C (Asha et al., 2003, Yu, 2002.9.17, ) Pk?2   scant
Name Synonym	greatwall (Yu et al., 2002, Archambault et al., 2007, Somma et al., 2008, Wang et al., 2011) Protein kinase-like 91C   scott of the antarctic (Glover, 1995)
Secondary FlyBase IDs
	FBgn0004461 FBgn0015538 FBgn0038650 FBgn0082209
References ( 49 )
Generate a list of	All references relating to this gene ( 49 )
List References by type	Research paper  ( 16 ) Supplementary material  ( 8 ) Review  ( 1 ) Personal communication to FlyBase  ( 12 ) Abstract  ( 4 ) FlyBase analysis  ( 1 ) Stock list  ( 1 ) Patent  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 ) Book  ( 1 )
Recent research papers ( 4 )
	Kim et al., 2012, Genetics 191(4): 1181--1197 Bypassing the Greatwall-Endosulfine Pathway: Plasticity of a Pivotal Cell-Cycle Regulatory Module in Drosophila melanogaster and Caenorhabditis elegans. [FBrf0219186] Soshnev et al., 2012, Nucleic Acids Res. 40(12): 5415--5431 Genome-wide studies of the multi-zinc finger Drosophila Suppressor of Hairy-wing protein in the ovary. [FBrf0218724] Rangone et al., 2011, PLoS Genet. 7(8): e1002225 Suppression of Scant Identifies Endos as a Substrate of Greatwall Kinase and a Negative Regulator of Protein Phosphatase 2A in Mitosis. [FBrf0214749] Wang et al., 2011, PLoS Genet. 7(8): e1002227 PP2A-Twins Is Antagonized by Greatwall and Collaborates with Polo for Cell Cycle Progression and Centrosome Attachment to Nuclei in Drosophila Embryos. [FBrf0214683] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...