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General Information
Symbol
Dmel\Pp2A-29B
Species
D. melanogaster
Name
Protein phosphatase 2A at 29B
Annotation Symbol
CG17291
Feature Type
FlyBase ID
FBgn0260439
Gene Model Status
Stock Availability
Gene Snapshot
Protein phosphatase 2A at 29B (Pp2A-29B) encodes the structural A subunit of the trimeric PP2A phosphatase enzyme. It links the catalytic C subunit encoded by mts and a variable regulatory B family subunit, which directs the enzyme to distinct substrates. The roles of Pp2A-29B product include centriole duplication, chromosome segregation, autophagy, axonal transport, growth regulation, and active zone stabilization. [Date last reviewed: 2019-02-28]
Also Known As
PP2A, PP2A-A, PP2A 29B
Key Links
Genomic Location
Cytogenetic map
Sequence location
2L:8,366,038..8,370,090 [+]
Recombination map
2-31
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Protein Family (UniProt)
Belongs to the phosphatase 2A regulatory subunit A family. (P36179)
Molecular Function (GO)
[Detailed GO annotations]
Experimental Evidence
-
Predictions / Assertions
Summaries
Pathway (FlyBase)
Negative Regulators of Hippo Signaling Pathway -
The Hippo signaling pathway is an intracellular kinase cascade in which hpo kinase in complex with sav, phosphorylates wts kinase which, in turn, phosphorylates yki transcriptional co-activator leading to its cytosolic retention. Negative regulators of the pathway promote the nuclear accumulation of yki, enhancing yki-mediated transcriptional regulation and the expression of genes that positively regulate tissue growth (Adapted from FBrf0224870).
Negative Regulators of Insulin-like Receptor Signaling Pathway -
Negative regulators of the Insulin-like Receptor signaling pathway suppress Insulin-like receptor (InR) activation or the activity of intracellular effectors. (Adapted from FBrf0232297, FBrf0230017 and FBrf0229989).
Negative Regulators of Hedgehog Signaling Pathway -
Negative regulators of hedgehog signaling down-regulation the pathway, resulting in the repression of transcription of hh-responsive genes.
Gene Group (FlyBase)
STRIATIN-INTERACTING PHOSPHATASE AND KINASE COMPLEX -
Striatin interacting phosphatase and kinase (STRIPAK)/STRIPAK-like complexes contain a protein phosphatase type 2A (mts), a striatin (Cka) and other regulatory subunits. Kinases, such as GckIII, are often associated with the complex (FBrf0211636). The STRIPAK complex has a regulatory role in many signaling pathways and cellular processes. (Adapted from FBrf0223805).
WRD-PROTEIN PHOSOPHATASE 2A COMPLEX -
wrd-PP2A is a serine/threonine phosphatase complex characterized by the presence of a wrd regulatory B' subunit. (Adapted from FBrf0232706.)
WDB-PROTEIN PHOSOPHATASE 2A COMPLEX -
wdb-PP2A is a serine/threonine phosphatase complex characterized by the presence of a wdb regulatory B' subunit. (Adapted from FBrf0232706.)
TWS-PROTEIN PHOSOPHATASE 2A COMPLEX -
tws-PP2A is a serine/threonine phosphatase complex characterized by the presence of a tws regulatory B subunit. (Adapted from FBrf0232706.)
Protein Function (UniProtKB)
The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit.
(UniProt, P36179)
Gene Model and Products
Number of Transcripts
5
Number of Unique Polypeptides
3

Please see the GBrowse view of Dmel\Pp2A-29B or the JBrowse view of Dmel\Pp2A-29B for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Gene model reviewed during 5.50
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0005088
2411
591
FBtr0100533
2342
591
FBtr0100535
2355
591
FBtr0306102
2588
650
FBtr0339789
2757
650
Additional Transcript Data and Comments
Reported size (kB)
7.5, 5.5, 2.8 (northern blot)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0099974
65.4
591
4.66
FBpp0099975
65.4
591
4.66
FBpp0293227
65.4
591
4.66
FBpp0297239
71.8
650
4.52
FBpp0308836
71.9
650
4.52
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

Additional Polypeptide Data and Comments
Reported size (kDa)
591 (aa); 65 (kD predicted)
Comments
External Data
Subunit Structure (UniProtKB)
PP2A exists in several trimeric forms, all of which consist of a core composed of a catalytic subunit associated with a 65 kDa regulatory subunit (PR65) (subunit A). The core complex associates with a third, variable subunit (subunit B), which confers distinct properties to the holoenzyme.
(UniProt, P36179)
Domain
Each HEAT repeat appears to consist of two alpha helices joined by a hydrophilic region, the intrarepeat loop. The repeat units may be arranged laterally to form a rod-like structure.
(UniProt, P36179)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Pp2A-29B using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Gene Ontology (15 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from sequence or structural similarity
inferred from biological aspect of ancestor with PANTHER:PTN000068596
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000068596
(assigned by GO_Central )
Biological Process (9 terms)
Terms Based on Experimental Evidence (8 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from sequence or structural similarity
Cellular Component (4 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
inferred from direct assay
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000068663
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000068663
(assigned by GO_Central )
inferred from sequence or structural similarity
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Pp2A-29B and mts proteins show the same cytoplasmic localization in embryos and imaginal discs as the tws protein. In syncytial blastoderm embryos the protein is found in the cytoplasmic region surrounding the cortical layer of nuclei. The protein is cytoplasmic in pole cells. In cycle 14 embryos, the protein is located throughout the cytoplasm of interphase cells, and throughout the whole cell as it progresses through mitosis. In dividing third larval instar brain neuroblasts, the protein is detected in the cytoplasm of interphase and mitotic cells.
Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Pp2A-29B in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 4 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Pp2A-29B
Transgenic constructs containing regulatory region of Pp2A-29B
Deletions and Duplications ( 0 )
Phenotypes
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
15 of 15
Yes
Yes
12 of 15
Yes
No
1 of 15
No
No
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
14 of 15
Yes
Yes
12 of 15
Yes
No
1 of 15
No
No
Rattus norvegicus (Norway rat) (3)
13 of 13
Yes
Yes
7 of 13
Yes
No
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (4)
8 of 12
Yes
Yes
6 of 12
Yes
No
1 of 12
No
No
1 of 12
No
Yes
Danio rerio (Zebrafish) (3)
13 of 15
Yes
Yes
13 of 15
Yes
Yes
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (1)
13 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (4)
9 of 9
Yes
Yes
8 of 9
Yes
No
8 of 9
Yes
No
1 of 9
No
No
Saccharomyces cerevisiae (Brewer's yeast) (1)
15 of 15
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
12 of 12
Yes
Yes
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG0919047Z )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091502XU )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W02SW )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X02PY )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G045V )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 2 )
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser

    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Subunit Structure (UniProtKB)
    PP2A exists in several trimeric forms, all of which consist of a core composed of a catalytic subunit associated with a 65 kDa regulatory subunit (PR65) (subunit A). The core complex associates with a third, variable subunit (subunit B), which confers distinct properties to the holoenzyme.
    (UniProt, P36179 )
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Gene Group - Pathway Membership (FlyBase)
    Negative Regulators of Hippo Signaling Pathway -
    The Hippo signaling pathway is an intracellular kinase cascade in which hpo kinase in complex with sav, phosphorylates wts kinase which, in turn, phosphorylates yki transcriptional co-activator leading to its cytosolic retention. Negative regulators of the pathway promote the nuclear accumulation of yki, enhancing yki-mediated transcriptional regulation and the expression of genes that positively regulate tissue growth (Adapted from FBrf0224870).
    Negative Regulators of Insulin-like Receptor Signaling Pathway -
    Negative regulators of the Insulin-like Receptor signaling pathway suppress Insulin-like receptor (InR) activation or the activity of intracellular effectors. (Adapted from FBrf0232297, FBrf0230017 and FBrf0229989).
    Negative Regulators of Hedgehog Signaling Pathway -
    Negative regulators of hedgehog signaling down-regulation the pathway, resulting in the repression of transcription of hh-responsive genes.
    External Data
    Linkouts
    KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2L
    Recombination map
    2-31
    Cytogenetic map
    Sequence location
    2L:8,366,038..8,370,090 [+]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    29B2-29B2
    Limits computationally determined from genome sequence between P{EP}EP646EP646&P{lacW}RpS13k09614 and P{lacW}l(2)k12914k12914&P{lacW}Akap200k07118a
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    29B1-29B2
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (13)
    Genomic Clones (15)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (260)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
    Commercially Available Antibodies
     
    Cell Signaling Technology - Commercial vendor for primary antibodies and antibody conjugates.
    Other Information
    Relationship to Other Genes
    Source for database identify of
    Source for database merge of
    Source for merge of: Pp2A-29B CG17291
    Additional comments
    The symbol "CG13383" has been used erroneously in FlyBase to name 2 adjacent annotations in different releases of the genome annotation. The two annotations represent the CSN8 and Pp2A-29B genes. In release 2 of the genome annotation, the "CG13383" annotation corresponded to the CSN8 open reading frame (ORF), and the annotation corresponding to Pp2A-29B was labelled "CG17291". In release 3.1 of the genome annotation, the "CG13383" annotation corresponded to the Pp2A-29B open reading frame (ORF). In release 3.2 of the genome annotation, the two genes were annotated as part of a dicistronic transcript represented by the "CG33297" annotation. In release 5.1 of the genome annotation, the two genes were annotated as monocistronic transcripts, as there was no longer sufficient evidence to support a processed dicistronic transcript, with the annotation representing the Pp2A-29B gene being labelled "CG17291" and the annotation representing the CSN8 gene being labelled "CG13383". Since the "CG13383" symbol has been used for two different annotations, the annotation for the CSN8 gene has been renamed to "CG42522" in release 5.13 of the genome annotation to avoid confusion.
    RT-PCR analysis of CSN8 and Pp2A-29B transcription provides no evidence that a dicistronic transcript containing both open reading frames is produced. Thus it is concluded that CSN8 is transcribed independently of Pp2A-29B and that a dicistronic transcript is unlikely or is present at undetectable levels.
    CSN8 and Pp2A-29B were annotated as being non-overlapping open reading frames derived from a processed dicistronic transcript in release 3.2 of the genome based on the LD10247 cDNA. However, this clone appears to be problematic and there is no longer sufficient evidence to support a processed dicistronic transcript, thus CSN8 and Pp2A-29B have been annotated as genes encoded by monocistronic transcripts in release 5.1 of the genome annotation.
    Other Comments
    Pp2A-29B is required for efficient recruitment of pericentriolar material.
    RNAi screen using dsRNA made from templates generated with primers directed against this gene causes defects in the formation of monastral bipolar spindles when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.
    S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.
    dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation.
    No distinct cDNA has been detected for CSN8, but a sequence putatively encoding CSN8 is found in the 5' untranslated region of the Pp2A-29B gene.
    Pp2A-29B has been molecularly cloned and developmental expression patterns examined.
    Origin and Etymology
    Discoverer
    Etymology
    Identification
    External Crossreferences and Linkouts ( 110 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    Flygut - An atlas of the Drosophila adult midgut
    FlyMine - An integrated database for Drosophila genomics
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    InterPro - A database of protein families, domains and functional sites
    KEGG Genes - Molecular building blocks of life in the genomic space.
    KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
    modMine - A data warehouse for the modENCODE project
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    Cell Signaling Technology
    Cell Signaling Technology - Commercial vendor for primary antibodies and antibody conjugates.
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Synonyms and Secondary IDs (25)
    Reported As
    Symbol Synonym
    Name Synonyms
    65 kDa regulatory subunit of protein phosphatase
    PP2A 65kD subunit A
    Protein phosphatase 2A at 29B
    protein phosphatase 2A
    protein phosphatase 2A at 29B
    protein phosphate 2A
    regulatory subunit A of type 2A protein phosphatase
    Secondary FlyBase IDs
    • FBgn0032028
    • FBgn0053297
    • FBtr0079644
    • FBpp0079260
    • FBtr0079645
    • FBpp0079261
    • FBtr0079646
    • FBpp0079262
    • FBtr0079647
    • FBpp0079263
    • FBgn0005776
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (122)