The symbol "CG13383" has been used erroneously in FlyBase to name 2 adjacent annotations in different releases of the genome annotation. The two annotations represent the CSN8
genes. In release 2 of the genome annotation, the "CG13383" annotation corresponded to the CSN8
open reading frame (ORF), and the annotation corresponding to Pp2A-29B
was labelled "CG17291". In release 3.1 of the genome annotation, the "CG13383" annotation corresponded to the Pp2A-29B
open reading frame (ORF). In release 3.2 of the genome annotation, the two genes were annotated as part of a dicistronic transcript represented by the "CG33297" annotation. In release 5.1 of the genome annotation, the two genes were annotated as monocistronic transcripts, as there was no longer sufficient evidence to support a processed dicistronic transcript, with the annotation representing the Pp2A-29B
gene being labelled "CG17291" and the annotation representing the CSN8
gene being labelled "CG13383". Since the "CG13383" symbol has been used for two different annotations, the annotation for the CSN8
gene has been renamed to "CG42522" in release 5.13 of the genome annotation to avoid confusion.
RT-PCR analysis of CSN8
transcription provides no evidence that a dicistronic transcript containing both open reading frames is produced. Thus it is concluded that CSN8
is transcribed independently of Pp2A-29B
and that a dicistronic transcript is unlikely or is present at undetectable levels.
were annotated as being non-overlapping open reading frames derived from a processed dicistronic transcript in release 3.2 of the genome based on the LD10247 cDNA. However, this clone appears to be problematic and there is no longer sufficient evidence to support a processed dicistronic transcript, thus CSN8
have been annotated as genes encoded by monocistronic transcripts in release 5.1 of the genome annotation.