PP2A, PP2A-A, PP2A 29B
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Gene model reviewed during 5.50
7.5, 5.5, 2.8 (northern blot)
591 (aa); 65 (kD predicted)
PP2A exists in several trimeric forms, all of which consist of a core composed of a catalytic subunit associated with a 65 kDa regulatory subunit (PR65) (subunit A). The core complex associates with a third, variable subunit (subunit B), which confers distinct properties to the holoenzyme.
Each HEAT repeat appears to consist of two alpha helices joined by a hydrophilic region, the intrarepeat loop. The repeat units may be arranged laterally to form a rod-like structure.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Pp2A-29B using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Pp2A-29B in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for merge of: Pp2A-29B CG17291
The symbol "CG13383" has been used erroneously in FlyBase to name 2 adjacent annotations in different releases of the genome annotation. The two annotations represent the CSN8 and Pp2A-29B genes. In release 2 of the genome annotation, the "CG13383" annotation corresponded to the CSN8 open reading frame (ORF), and the annotation corresponding to Pp2A-29B was labelled "CG17291". In release 3.1 of the genome annotation, the "CG13383" annotation corresponded to the Pp2A-29B open reading frame (ORF). In release 3.2 of the genome annotation, the two genes were annotated as part of a dicistronic transcript represented by the "CG33297" annotation. In release 5.1 of the genome annotation, the two genes were annotated as monocistronic transcripts, as there was no longer sufficient evidence to support a processed dicistronic transcript, with the annotation representing the Pp2A-29B gene being labelled "CG17291" and the annotation representing the CSN8 gene being labelled "CG13383". Since the "CG13383" symbol has been used for two different annotations, the annotation for the CSN8 gene has been renamed to "CG42522" in release 5.13 of the genome annotation to avoid confusion.
RT-PCR analysis of CSN8 and Pp2A-29B transcription provides no evidence that a dicistronic transcript containing both open reading frames is produced. Thus it is concluded that CSN8 is transcribed independently of Pp2A-29B and that a dicistronic transcript is unlikely or is present at undetectable levels.
CSN8 and Pp2A-29B were annotated as being non-overlapping open reading frames derived from a processed dicistronic transcript in release 3.2 of the genome based on the LD10247 cDNA. However, this clone appears to be problematic and there is no longer sufficient evidence to support a processed dicistronic transcript, thus CSN8 and Pp2A-29B have been annotated as genes encoded by monocistronic transcripts in release 5.1 of the genome annotation.
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes defects in the formation of monastral bipolar spindles when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.
S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.
dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation.
Pp2A-29B has been molecularly cloned and developmental expression patterns examined.