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Gene Dmel\spdo

General Information
Symbol	Dmel\spdo	Species	D. melanogaster
Name	sanpodo	Annotation symbol	CG31020
Feature type	protein_coding_gene	FlyBase ID	FBgn0260440
Gene Model Status	Current	Stock availability	7 publicly available
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	99F6-99F6	Sequence location	3R:26,302,406..26,304,571 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene sanpodo is referred to in FlyBase by the symbol Dmel\spdo (CG31020, FBgn0260440). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: Notch binding; protein binding. There is experimental evidence that it is involved in the biological process: negative regulation of Notch signaling pathway; heart morphogenesis; sensory organ development; Notch signaling pathway; sensory organ precursor cell division; asymmetric cell division. 35 alleles are reported. The phenotypes of these alleles are annotated with: organ system; organ system subdivision; ISN innervated muscle of A1-7; hypodermal muscle of larval abdomen; embryonic/larval neuron; abdominal dorsal bipolar neuron dbp; peripheral nervous system; adult segment; non-connected developing system; sense organ. It has one annotated transcript and one annotated polypeptide. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 06-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system. Comments on Affy2 ProbeSet: ProbeSet 1637254_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of spdo. Gene sequence location is 3R:26302406..26304571.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 99F6-99F6   Limits computationally determined from genome sequence between P{PZ}ncd05884 and P{lacW}l(3)s2500s2500
Experimentally Determined Cytological Location
Cytogenetic map Notes References 99F1-100B5   (determined by in situ hybridisation)   (Salzberg et al., 1994)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\spdo for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.44
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) spdo-RA FBtr0085651  NM_170493   2087   565
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein spdo-PA   FBpp0085013   63.8   565   8.71     NP_733372   AAN14235
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014297 AAN14235   AY069737   BI164912   BI231733   BT001576 AAN71331   BT057990   EC211423   EC246757
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q8IMI5 Q8IGV5
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References regulatory region FBsf0000435900 3R:26,300,357..26,301,356 evidence=Reporter construct (in vivo) (Rouault et al., 2010, Halfon, 2012.6.19) regulatory region FBsf0000435902 3R:26,301,660..26,302,659 evidence=Reporter construct (in vivo) (Rouault et al., 2010, Halfon, 2012.6.19) regulatory region FBsf0000436577 3R:26,300,453..26,301,406 evidence=Reporter construct (in vivo) (Aerts et al., 2010, Halfon, 2012.6.19)
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage   presumptive embryonic/larval central nervous system   (O'Connor-Giles and Skeath, 2003) embryonic/larval central nervous system   (O'Connor-Giles and Skeath, 2003) presumptive embryonic/larval peripheral nervous system   (O'Connor-Giles and Skeath, 2003) embryonic/larval peripheral nervous system   (O'Connor-Giles and Skeath, 2003) mesoderm   (O'Connor-Giles and Skeath, 2003)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage 10 -- 13   embryonic neuroblast   • plasma membrane (O'Connor-Giles and Skeath, 2003) embryonic ganglion mother cell   • plasma membrane (O'Connor-Giles and Skeath, 2003) mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010)
Additional Descriptive Data
	spdo protein is expressed in all embryonic cells known to undergo asymmetric divisions. (O'Connor-Giles and Skeath, 2003)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
cortical cytoskeleton inferred from direct assay (Roegiers et al., 2005) cytoplasm inferred from direct assay (O'Connor-Giles and Skeath, 2003) colocalizes_with cytoplasmic vesicle inferred from direct assay (Jafar-Nejad et al., 2005) early endosome inferred from direct assay (Hutterer and Knoblich, 2005) late endosome inferred from direct assay (Hutterer and Knoblich, 2005) plasma membrane inferred from direct assay (O'Connor-Giles and Skeath, 2003, Roegiers et al., 2005, Hutterer and Knoblich, 2005) colocalizes_with plasma membrane inferred from direct assay (Jafar-Nejad et al., 2005)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    240.375 Larval Midgut    31.3 Larval Hindgut    9.2 Larval Malpighian Tubules    11.3 Larval Fat Body    12.5 Larval Salivary Gland    6.9 Larval Trachea    6.325 Larval Carcass    6.5 Adult Head    8.2 Adult Eye    5.95 Adult Brain    5.9 Adult Thoracic-Abdominal Ganglion    11.2 Adult Crop    12.4 Adult Midgut    34.9 Adult Hindgut    14.4 Adult Malpighian Tubules    9.5 Adult Fat Body    14.9 Adult Salivary Gland    13.2 Adult Heart    4.75 Adult VirginFemale Spermatheca    19.9 Adult InseminatedFemale Spermatheca    18.1 Adult Ovary    6.6 Adult Testis    4.8 Adult Male Accessory Gland    55.2 Adult Carcass    13.8 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    240.375 Larval Midgut    31.3 Larval Hindgut    9.2 Larval Malpighian Tubules    11.3 Larval Fat Body    12.5 Larval Salivary Gland    6.9 Larval Trachea    6.325 Larval Carcass    6.5 Adult Head    8.2 Adult Eye    5.95 Adult Brain    5.9 Adult Thoracic-Abdominal Ganglion    11.2 Adult Crop    12.4 Adult Midgut    34.9 Adult Hindgut    14.4 Adult Malpighian Tubules    9.5 Adult Fat Body    14.9 Adult Salivary Gland    13.2 Adult Heart    4.75 Adult VirginFemale Spermatheca    19.9 Adult InseminatedFemale Spermatheca    18.1 Adult Ovary    6.6 Adult Testis    4.8 Adult Male Accessory Gland    55.2 Adult Carcass    13.8 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    240.375 Larval Midgut    31.3 Larval Hindgut    9.2 Larval Malpighian Tubules    11.3 Larval Fat Body    12.5 Larval Salivary Gland    6.9 Larval Trachea    6.325 Larval Carcass    6.5 Adult Head    8.2 Adult Eye    5.95 Adult Brain    5.9 Adult Thoracic-Abdominal Ganglion    11.2 Adult Crop    12.4 Adult Midgut    34.9 Adult Hindgut    14.4 Adult Malpighian Tubules    9.5 Adult Fat Body    14.9 Adult Salivary Gland    13.2 Adult Heart    4.75 Adult VirginFemale Spermatheca    19.9 Adult InseminatedFemale Spermatheca    18.1 Adult Ovary    6.6 Adult Testis    4.8 Adult Male Accessory Gland    55.2 Adult Carcass    13.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    240.375 Larval Midgut    31.3 Larval Hindgut    9.2 Larval Malpighian Tubules    11.3 Larval Fat Body    12.5 Larval Salivary Gland    6.9 Larval Trachea    6.325 Larval Carcass    6.5 Adult Head    8.2 Adult Eye    5.95 Adult Brain    5.9 Adult Thoracic-Abdominal Ganglion    11.2 Adult Crop    12.4 Adult Midgut    34.9 Adult Hindgut    14.4 Adult Malpighian Tubules    9.5 Adult Fat Body    14.9 Adult Salivary Gland    13.2 Adult Heart    4.75 Adult VirginFemale Spermatheca    19.9 Adult InseminatedFemale Spermatheca    18.1 Adult Ovary    6.6 Adult Testis    4.8 Adult Male Accessory Gland    55.2 Adult Carcass    13.8 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    240.375 Larval Midgut    31.3 Larval Hindgut    9.2 Larval Malpighian Tubules    11.3 Larval Fat Body    12.5 Larval Salivary Gland    6.9 Larval Trachea    6.325 Larval Carcass    6.5 Adult Head    8.2 Adult Eye    5.95 Adult Brain    5.9 Adult Thoracic-Abdominal Ganglion    11.2 Adult Crop    12.4 Adult Midgut    34.9 Adult Hindgut    14.4 Adult Malpighian Tubules    9.5 Adult Fat Body    14.9 Adult Salivary Gland    13.2 Adult Heart    4.75 Adult VirginFemale Spermatheca    19.9 Adult InseminatedFemale Spermatheca    18.1 Adult Ovary    6.6 Adult Testis    4.8 Adult Male Accessory Gland    55.2 Adult Carcass    13.8 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    240.375 Larval Midgut    31.3 Larval Hindgut    9.2 Larval Malpighian Tubules    11.3 Larval Fat Body    12.5 Larval Salivary Gland    6.9 Larval Trachea    6.325 Larval Carcass    6.5 Adult Head    8.2 Adult Eye    5.95 Adult Brain    5.9 Adult Thoracic-Abdominal Ganglion    11.2 Adult Crop    12.4 Adult Midgut    34.9 Adult Hindgut    14.4 Adult Malpighian Tubules    9.5 Adult Fat Body    14.9 Adult Salivary Gland    13.2 Adult Heart    4.75 Adult VirginFemale Spermatheca    19.9 Adult InseminatedFemale Spermatheca    18.1 Adult Ovary    6.6 Adult Testis    4.8 Adult Male Accessory Gland    55.2 Adult Carcass    13.8 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    240.375 Larval Midgut    31.3 Larval Hindgut    9.2 Larval Malpighian Tubules    11.3 Larval Fat Body    12.5 Larval Salivary Gland    6.9 Larval Trachea    6.325 Larval Carcass    6.5 Adult Head    8.2 Adult Eye    5.95 Adult Brain    5.9 Adult Thoracic-Abdominal Ganglion    11.2 Adult Crop    12.4 Adult Midgut    34.9 Adult Hindgut    14.4 Adult Malpighian Tubules    9.5 Adult Fat Body    14.9 Adult Salivary Gland    13.2 Adult Heart    4.75 Adult VirginFemale Spermatheca    19.9 Adult InseminatedFemale Spermatheca    18.1 Adult Ovary    6.6 Adult Testis    4.8 Adult Male Accessory Gland    55.2 Adult Carcass    13.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    240.375 Larval Midgut    31.3 Larval Hindgut    9.2 Larval Malpighian Tubules    11.3 Larval Fat Body    12.5 Larval Salivary Gland    6.9 Larval Trachea    6.325 Larval Carcass    6.5 Adult Head    8.2 Adult Eye    5.95 Adult Brain    5.9 Adult Thoracic-Abdominal Ganglion    11.2 Adult Crop    12.4 Adult Midgut    34.9 Adult Hindgut    14.4 Adult Malpighian Tubules    9.5 Adult Fat Body    14.9 Adult Salivary Gland    13.2 Adult Heart    4.75 Adult VirginFemale Spermatheca    19.9 Adult InseminatedFemale Spermatheca    18.1 Adult Ovary    6.6 Adult Testis    4.8 Adult Male Accessory Gland    55.2 Adult Carcass    13.8 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    8.2   NA  Eye    5.95   NA  Brain    5.9   240.375  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    no informative data   NA  Crop    no informative data   31.3  Midgut    34.9   9.2  Hindgut    no informative data   no informative data  Malpighian Tubules    9.5   no informative data  Fat Body    no informative data   6.9  Salivary Gland    no informative data   NA  Heart    4.75   6.325  Trachea    NA   NA  VirginFemale Spermatheca    19.9   NA  InseminatedFemale Spermatheca    18.1   NA  Ovary    6.6   NA  Testis    4.8   NA  Male Accessory Gland    55.2   6.5  Carcass    no informative data FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    5 central nervous system, larvae L3    59 central nervous system, pupae P8    1 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    0 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    2 fat body, pupae P8    2 carcass, larvae L3 wandering    9 carcass, 1-day adult    0 carcass, 4-day adult    1 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    5 central nervous system, larvae L3  (59) central nervous system, pupae P8    1 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    0 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    2 fat body, pupae P8    2 carcass, larvae L3 wandering    9 carcass, 1-day adult    0 carcass, 4-day adult    1 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    5 central nervous system, larvae L3    59 central nervous system, pupae P8    1 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    0 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    2 fat body, pupae P8    2 carcass, larvae L3 wandering    9 carcass, 1-day adult    0 carcass, 4-day adult    1 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    5 central nervous system, larvae L3    59 central nervous system, pupae P8    1 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    0 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    2 fat body, pupae P8    2 carcass, larvae L3 wandering    9 carcass, 1-day adult    0 carcass, 4-day adult    1 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    5 central nervous system, larvae L3    59 central nervous system, pupae P8    1 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    0 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    2 fat body, pupae P8    2 carcass, larvae L3 wandering    9 carcass, 1-day adult    0 carcass, 4-day adult    1 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    5 central nervous system, larvae L3  (59) central nervous system, pupae P8    1 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    0 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    2 fat body, pupae P8    2 carcass, larvae L3 wandering    9 carcass, 1-day adult    0 carcass, 4-day adult    1 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    5 central nervous system, larvae L3    59 central nervous system, pupae P8    1 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    0 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    2 fat body, pupae P8    2 carcass, larvae L3 wandering    9 carcass, 1-day adult    0 carcass, 4-day adult    1 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    5 central nervous system, larvae L3    59 central nervous system, pupae P8    1 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    0 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    1 fat body, larvae L3 wandering    1 fat body, white prepupae    2 fat body, pupae P8    2 carcass, larvae L3 wandering    9 carcass, 1-day adult    0 carcass, 4-day adult    1 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 06-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    8 embryo 04-06hr    29 embryo 06-08hr    83 embryo 08-10hr    62 embryo 10-12hr    31 embryo 12-14hr    16 embryo 14-16hr    6 embryo 16-18hr    2 embryo 18-20hr    3 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    3 white prepupae new    4 white prepupae 12hr    5 white prepupae 24hr    4 pupae 2d postWPP    1 pupae 3d postWPP    1 pupae 4d postWPP    1 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    8 embryo 04-06hr  (29) embryo 06-08hr  (83) embryo 08-10hr  (62) embryo 10-12hr  (31) embryo 12-14hr    16 embryo 14-16hr    6 embryo 16-18hr    2 embryo 18-20hr    3 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    3 white prepupae new    4 white prepupae 12hr    5 white prepupae 24hr    4 pupae 2d postWPP    1 pupae 3d postWPP    1 pupae 4d postWPP    1 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    8 embryo 04-06hr    29 embryo 06-08hr    83 embryo 08-10hr    62 embryo 10-12hr    31 embryo 12-14hr    16 embryo 14-16hr    6 embryo 16-18hr    2 embryo 18-20hr    3 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    3 white prepupae new    4 white prepupae 12hr    5 white prepupae 24hr    4 pupae 2d postWPP    1 pupae 3d postWPP    1 pupae 4d postWPP    1 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    8 embryo 04-06hr    29 embryo 06-08hr    83 embryo 08-10hr    62 embryo 10-12hr    31 embryo 12-14hr    16 embryo 14-16hr    6 embryo 16-18hr    2 embryo 18-20hr    3 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    3 white prepupae new    4 white prepupae 12hr    5 white prepupae 24hr    4 pupae 2d postWPP    1 pupae 3d postWPP    1 pupae 4d postWPP    1 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    8 embryo 04-06hr    29 embryo 06-08hr    83 embryo 08-10hr    62 embryo 10-12hr    31 embryo 12-14hr    16 embryo 14-16hr    6 embryo 16-18hr    2 embryo 18-20hr    3 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    3 white prepupae new    4 white prepupae 12hr    5 white prepupae 24hr    4 pupae 2d postWPP    1 pupae 3d postWPP    1 pupae 4d postWPP    1 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    8 embryo 04-06hr  29 embryo 06-08hr  (83) embryo 08-10hr  (62) embryo 10-12hr  31 embryo 12-14hr    16 embryo 14-16hr    6 embryo 16-18hr    2 embryo 18-20hr    3 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    3 white prepupae new    4 white prepupae 12hr    5 white prepupae 24hr    4 pupae 2d postWPP    1 pupae 3d postWPP    1 pupae 4d postWPP    1 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    8 embryo 04-06hr    29 embryo 06-08hr    83 embryo 08-10hr    62 embryo 10-12hr    31 embryo 12-14hr    16 embryo 14-16hr    6 embryo 16-18hr    2 embryo 18-20hr    3 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    3 white prepupae new    4 white prepupae 12hr    5 white prepupae 24hr    4 pupae 2d postWPP    1 pupae 3d postWPP    1 pupae 4d postWPP    1 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    8 embryo 04-06hr    29 embryo 06-08hr    83 embryo 08-10hr    62 embryo 10-12hr    31 embryo 12-14hr    16 embryo 14-16hr    6 embryo 16-18hr    2 embryo 18-20hr    3 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    3 white prepupae new    4 white prepupae 12hr    5 white prepupae 24hr    4 pupae 2d postWPP    1 pupae 3d postWPP    1 pupae 4d postWPP    1 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    3 Schneider line 2 Sg4    2 embryonic 1182-4H    3 embryonic GM2    0 embryonic Kc167    1 embryonic S1    19 embryonic S3    3 leg disc CME L1    3 wing disc CME-W2    1 wing disc ML-DmD8    2 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    7 wing disc ML-DmD21    1 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    1 ovary fGS/OSS    1 ovary OSC    1 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    3 Schneider line 2 Sg4    2 embryonic 1182-4H    3 embryonic GM2    0 embryonic Kc167    1 embryonic S1    19 embryonic S3    3 leg disc CME L1    3 wing disc CME-W2    1 wing disc ML-DmD8    2 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    7 wing disc ML-DmD21    1 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    1 ovary fGS/OSS    1 ovary OSC    1 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    3 Schneider line 2 Sg4    2 embryonic 1182-4H    3 embryonic GM2    0 embryonic Kc167    1 embryonic S1    19 embryonic S3    3 leg disc CME L1    3 wing disc CME-W2    1 wing disc ML-DmD8    2 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    7 wing disc ML-DmD21    1 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    1 ovary fGS/OSS    1 ovary OSC    1 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    3 Schneider line 2 Sg4    2 embryonic 1182-4H    3 embryonic GM2    0 embryonic Kc167    1 embryonic S1    19 embryonic S3    3 leg disc CME L1    3 wing disc CME-W2    1 wing disc ML-DmD8    2 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    7 wing disc ML-DmD21    1 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    1 ovary fGS/OSS    1 ovary OSC    1 ovary OSS    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    3 Schneider line 2 Sg4    2 embryonic 1182-4H    3 embryonic GM2    0 embryonic Kc167    1 embryonic S1    19 embryonic S3    3 leg disc CME L1    3 wing disc CME-W2    1 wing disc ML-DmD8    2 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    7 wing disc ML-DmD21    1 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    1 ovary fGS/OSS    1 ovary OSC    1 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    3 Schneider line 2 Sg4    2 embryonic 1182-4H    3 embryonic GM2    0 embryonic Kc167    1 embryonic S1    19 embryonic S3    3 leg disc CME L1    3 wing disc CME-W2    1 wing disc ML-DmD8    2 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    7 wing disc ML-DmD21    1 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    1 ovary fGS/OSS    1 ovary OSC    1 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    3 Schneider line 2 Sg4    2 embryonic 1182-4H    3 embryonic GM2    0 embryonic Kc167    1 embryonic S1    19 embryonic S3    3 leg disc CME L1    3 wing disc CME-W2    1 wing disc ML-DmD8    2 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    7 wing disc ML-DmD21    1 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    1 ovary fGS/OSS    1 ovary OSC    1 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    3 Schneider line 2 Sg4    2 embryonic 1182-4H    3 embryonic GM2    0 embryonic Kc167    1 embryonic S1    19 embryonic S3    3 leg disc CME L1    3 wing disc CME-W2    1 wing disc ML-DmD8    2 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    7 wing disc ML-DmD21    1 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    1 ovary fGS/OSS    1 ovary OSC    1 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    5 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    4 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    5 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    4 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    5 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    4 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    5 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    4 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    5 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    4 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    5 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    4 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    5 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    4 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    5 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    1 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    4 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    1 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (Graveley et al., 2011) mE1_20_mRNA_expression_cluster_06
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0260440
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal (with spdo3R6) spdoC55 lethal (with spdoC55) spdo3R6 lethal | embryonic stage spdoG104 spdoH7 spdoK433 spdounspecified lethal | embryonic stage | recessive spdoS097002b lethal | recessive spdoC55 spdoK433 spdoS097002b viable, with Scer\GAL4pnr-MD237 spdoGD3130 Other Phenotypes Allele neuroanatomy defective spdoC55 spdoG104 neuroanatomy defective | recessive | somatic clone spdoG104 size defective spdoScer\UAS.cOa visible, with Scer\GAL4pnr-MD237 spdoGD3130 visible | somatic clone spdoC55 Phenotype manifest in Allele abdominal 1 basiconical sensillum dbd & embryonic glial cell spdoG104 spdoK433 abdominal 1 dorsal acute muscle 3 spdoK433 abdominal 1 dorsal bipolar neuron dbp spdoG104 spdoK433 abdominal 1 dorsal oblique muscle 5 spdoK433 abdominal 2 basiconical sensillum dbd & embryonic glial cell spdoG104 spdoK433 abdominal 2 dorsal acute muscle 3 spdoK433 abdominal 2 dorsal bipolar neuron dbp spdoG104 spdoK433 abdominal 2 dorsal oblique muscle 5 spdoK433 abdominal 3 basiconical sensillum dbd & embryonic glial cell spdoG104 spdoK433 abdominal 3 dorsal acute muscle 3 spdoK433 abdominal 3 dorsal bipolar neuron dbp spdoG104 spdoK433 abdominal 3 dorsal oblique muscle 5 spdoK433 abdominal 4 basiconical sensillum dbd & embryonic glial cell spdoG104 spdoK433 abdominal 4 dorsal acute muscle 3 spdoK433 abdominal 4 dorsal bipolar neuron dbp spdoG104 spdoK433 abdominal 4 dorsal oblique muscle 5 spdoK433 abdominal 5 basiconical sensillum dbd & embryonic glial cell spdoG104 spdoK433 abdominal 5 dorsal acute muscle 3 spdoK433 abdominal 5 dorsal bipolar neuron dbp spdoG104 spdoK433 abdominal 5 dorsal oblique muscle 5 spdoK433 abdominal 6 basiconical sensillum dbd & embryonic glial cell spdoG104 spdoK433 abdominal 6 dorsal acute muscle 3 spdoK433 abdominal 6 dorsal bipolar neuron dbp spdoG104 spdoK433 abdominal 6 dorsal oblique muscle 5 spdoK433 abdominal 7 basiconical sensillum dbd & embryonic glial cell spdoG104 spdoK433 abdominal 7 dorsal acute muscle 3 spdoK433 abdominal 7 dorsal bipolar neuron dbp spdoG104 spdoK433 abdominal 7 dorsal oblique muscle 5 spdoK433 abdominal dorsal bipolar neuron dbp | ectopic spdoC55 abdominal dorsal bipolar neuron dbp | embryonic stage | precursor spdounspecified abdominal dorsal bipolar neuron dbp | embryonic stage | supernumerary spdounspecified aCC neuron spdoG104 anterior commissure spdoC55 spdoG104 spdoK433 cardioblast spdoC55 chaeta | somatic clone spdoC55 CNS glial cell spdoC55 spdoG104 spdoK433 dMP2 neuron spdoG104 dMP2 neuron | ectopic spdoG104 dorsal multidendritic neuron | ectopic spdoC55 embryonic/larval neuroblast | somatic clone spdoG104 embryonic/larval pericardial cell spdoK433 embryonic heart cardioblast spdoG104 spdounspecified embryonic heart cardioblast | embryonic stage 16 | supernumerary, with Scer\GAL4twi.PG spdoScer\UAS.cOa embryonic pericardial cell spdoG104 embryonic pericardial cell | embryonic stage 16 | supernumerary, with Scer\GAL4twi.PG spdoScer\UAS.cOa embryonic peripheral nervous system & glial cell spdoC55 spdoH7 spdounspecified eo neuron | somatic clone spdoC55 EW1 neuron | ectopic spdoG104 external sensory organ spdoG104 spdoK433 external sensory organ precursor cell | ectopic | third instar larval stage, with Scer\GAL4ptc-559.1 spdoScer\UAS.cOa external sensory organ precursor cell IIb spdoG104 spdoK433 external sensory organ precursor cell IIb | somatic clone | supernumerary spdo3R6 external sensory organ precursor cell IIIb | somatic clone spdo3R6 frontal bristle | somatic clone spdo3R6 GW neuron spdoG104 interommatidial bristle | somatic clone spdo3R6 longitudinal connective spdoC55 spdoG104 spdoK433 spdounspecified macrochaeta & mesothoracic tergum | somatic clone spdoG104 macrochaeta | somatic clone spdoC55 mesothoracic tergum, with Scer\GAL4pnr-MD237 spdoKK103906 multidendritic neuron spdoG104 spdoK433 multidendritic neuron | precursor spdoG104 spdoK433 neuron | embryonic stage spdoS097002b pCC neuron spdoG104 peripheral nervous system spdoG104 spdoK433 peripheral nervous system precursor cluster spdoH7 spdounspecified peripheral nervous system precursor cluster & neuron | supernumerary spdoH7 spdounspecified posterior commissure spdoC55 spdoG104 spdoK433 presumptive embryonic/larval central nervous system spdoG104 spdoS097002b spdounspecified presumptive embryonic/larval peripheral nervous system spdoC55 RP2 motor neuron spdoG104 RP2 motor neuron | supernumerary spdodsRNA.cOa RP2sib neuron spdoG104 scutellar bristle | ectopic, with Scer\GAL4ptc-559.1 spdoScer\UAS.cOa secondary neuroblast | somatic clone spdoG104 thecogen cell spdoG104 spdoK433 thecogen cell & peripheral nervous system spdoC55 spdoK433 tormogen cell, with Scer\GAL4pnr-MD237 spdoGD3130 trichogen cell | supernumerary, with Scer\GAL4pnr-MD237 spdoGD3130 U neuron spdoG104 spdodsRNA.cOa Usib neuron spdoG104 vMP2 neuron spdoG104 wing, with Scer\GAL4nub-AC-62 spdoScer\UAS.cOa wing margin, with Scer\GAL4nub-AC-62 spdoScer\UAS.cOa wing vein, with Scer\GAL4nub-AC-62 spdoScer\UAS.cOa
Classical Alleles ( 16 )
For All Classical Alleles Show
Allele of spdo Class Mutagen Stocks Known lesion spdoC55 loss of function allele ethyl methanesulfonate 1 Yes spdoG104 loss of function allele ethyl methanesulfonate 1 Yes spdo3R6 hypomorphic allele - genetic evidence ethyl methanesulfonate 0 Yes spdoAB153 0 Yes spdoAC81 0 Yes spdoAC85 0 Yes spdoH7 loss of function allele 0 Yes spdoK433 loss of function allele ethyl methanesulfonate 0 Yes spdoOO3 0 Yes spdoP46 0 Yes spdoS097002b Delta2-3 P-element activity 0 Yes spdounspecified ethyl methanesulfonate 0 -- spdoVV86 0 Yes spdoYY233 0 -- spdoZ143 0 Yes spdoZZ213 0 Yes
Alleles Carried on Transgenic Constructs ( 19 )
For All Alleles Carried on Transgenic Constructs Show
Allele of spdo Class Mutagen Stocks Known lesion spdoGD3130 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes spdoJF01806 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes spdoKK103906 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes spdoScer\UAS.cOa in vitro construct - regulatory fusion 1 Yes spdo1-424.Scer\UAS.T:Mmus\Cd8a,T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes spdodsRNA.cOa in vitro construct - RNAi 0 Yes spdoL2.Scer\UAS.T:Disc\RFP-mCherry in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes spdoNIG.31020R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes spdoNPAA.Scer\UAS.T:Avic\GFP-EGFP in vitro construct - amino acid replacement 0 Yes spdoScer\UAS.T:Avic\GFP-EGFP 0 Yes spdoScer\UAS.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes spdoScer\UAS.T:Disc\RFP-mCherry in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes spdoΔ1-180.Scer\UAS.T:Avic\GFP-EGFP in vitro construct - deletion 0 Yes spdoΔ1-424.Scer\UAS.T:Avic\GFP-EGFP in vitro construct - deletion 0 Yes spdoΔ18.Scer\UAS.T:Disc\RFP-mCherry in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - deletion 0 Yes spdoΔ196-255.Scer\UAS.T:Avic\GFP-EGFP in vitro construct - deletion 0 Yes spdoΔ424-565.Scer\UAS.T:Avic\GFP-EGFP in vitro construct - deletion 0 Yes spdoΔN18.Scer\UAS.T:Avic\GFP-EGFP in vitro construct - deletion 0 Yes spdoΔNm.Scer\UAS.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - deletion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)Exel6215 (Ryder, 2004.4.26) Df(3R)Exel6216 (Ryder, 2004.4.26, Abrams et al., 2006) Df(3R)tll-g (Dye et al., 1998, Salzberg et al., 1994) Df(3R)ZZ27 (Dye et al., 1998, Park et al., 1998, Skeath, 2004) T(Y;3)R133 (BDGP Project Members, 1994-1999)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD3130} NA P{KK103906} NA P{NIG.31020R} NA P{TRiP.JF01806} NA P{UAS-Cherry-spdo.L2} NA P{UAS-spdo.GFP.FL} NA P{UAS-spdo.GFP.ΔNm} NA P{UAS-spdo.GFP} NA P{UAS-spdo.mCD8.1-424.GFP} NA P{UAS-Spdo.mCherry} NA P{UAS-spdo.NPAA.GFP} NA P{UAS-spdo.O} NA P{UAS-spdo.Δ1-180.GFP} NA P{UAS-spdo.Δ1-424.GFP} NA P{UAS-Spdo.Δ18.mCherry} NA P{UAS-spdo.Δ196-255.GFP} NA P{UAS-spdo.Δ424-565.GFP} NA P{UAS-spdo.ΔN18.GFP} NA heat-shock construct P{HS-tmod.E42} NA
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 16 unique terms )
Terms Based on Experimental Evidence ( 14 terms )
Molecular Function
CV term Evidence References
Notch binding inferred from physical interaction with N (O'Connor-Giles and Skeath, 2003) protein binding inferred from physical interaction with numb (O'Connor-Giles and Skeath, 2003)
Biological Process
CV term Evidence References
asymmetric cell division inferred from direct assay (Babaoglan et al., 2009) inferred from mutant phenotype (O'Connor-Giles and Skeath, 2003) heart morphogenesis inferred from mutant phenotype (Albrecht et al., 2006) negative regulation of Notch signaling pathway inferred from mutant phenotype (Babaoglan et al., 2009) Notch signaling pathway inferred from genetic interaction with N AND inferred from physical interaction with N (O'Connor-Giles and Skeath, 2003) sensory organ development inferred from mutant phenotype (Roegiers et al., 2005) sensory organ precursor cell division inferred from mutant phenotype (Babaoglan et al., 2009, Hutterer and Knoblich, 2005)
Cellular Component
CV term Evidence References
cortical cytoskeleton inferred from direct assay (Roegiers et al., 2005) cytoplasm inferred from direct assay (O'Connor-Giles and Skeath, 2003) colocalizes_with cytoplasmic vesicle inferred from direct assay (Jafar-Nejad et al., 2005) early endosome inferred from direct assay (Hutterer and Knoblich, 2005) late endosome inferred from direct assay (Hutterer and Knoblich, 2005) plasma membrane inferred from direct assay (O'Connor-Giles and Skeath, 2003, Roegiers et al., 2005, Hutterer and Knoblich, 2005) colocalizes_with plasma membrane inferred from direct assay (Jafar-Nejad et al., 2005)
Terms Based on Predictions or Assertions ( 2 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
peripheral nervous system development non-traceable author statement (Prokopenko et al., 2000) traceable author statement (Salzberg et al., 1994)
Cellular Component
CV term Evidence References
integral to membrane inferred from sequence or structural similarity (O'Connor-Giles and Skeath, 2003)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
spdo - numb anti tag coimmunoprecipitation, anti tag western blot (Song and Lu, 2012)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions spdo allele Gene References spdoG104 numb (Lundell et al., 2003) spdoH7 numb (Dye et al., 1998) tmod (Dye et al., 1998) unspecified numb (Skeath and Doe, 1998)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 76878 DroID - A comprehensive database of gene and protein interactions. • FBgn0260440 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 318559 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q8IMI5
Orthologs
OrthoDB Orthologs (13) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG62FT39 Dipteran inclusive ortholog search EOG62BXJ0 Insect inclusive ortholog search EOG65TBHF Arthropod inclusive ortholog search No orthologs identified Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (EOG62FT39)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\spdo     Drosophila yakuba   Dyak\GE10880 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA15944     Drosophila persimilis   Dper\GL13461 Y   Drosophila virilis   Dvir\GJ24501 Y   Drosophila mojavensis   Dmoj\GI24859 Y   Drosophila grimshawi   Dgri\GH14100 Y
Orthologs in non-Drosophila Dipterans (EOG62BXJ0)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Anopheles gambiae Malaria mosquito  Agam\AGAP002689   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ002901
Orthologs in non-Dipteran Insects (EOG65TBHF)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Acyrthosiphon pisum Pea aphid  Apim\ACYPI45608   Bombyx mori Silkmoth  Bmor\BGIBMGA009789   Pediculus humanus Human body louse  Phum\PHUM065540   Tribolium castaneum Red flour beetle  Tcas\TC010201
Orthologs in non-Insect Arthropods (None identified)
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (5) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila yakuba Dyak\GE10880 Drosophila persimilis Dper\GL13461 Drosophila virilis Dvir\GJ24501 Drosophila mojavensis Dmoj\GI24859 Drosophila grimshawi Dgri\GH14100
Stocks & Reagents
Stocks Listed in FlyBase ( 7 )
Bloomington	9934 w*; P{UAS-spdo.O}G14 31227 y1 v1; P{TRiP.JF01806}attP2 9933 y1 w*; P{neoFRT}82B es spdoG104/TM3, Sb1 4374 y1 w1; P{lacW}64A th1 st1 cu1 es spdoC55 ca1/TM6B, P{iab-2(1.7)lacZ}6B, Tb+
VDRC	v104092 P{KK103906}VIE-260B v6858 w1118 P{GD3130}v6858 v6856 w1118; P{GD3130}v6856/TM3
Genomic Clones ( 1 )
	BACR48G16 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 6 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LP05501 RE23355
Other clones	FI04474
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RE04681
Other clones	55734 66330
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0260440 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 318559
Antibody Information
	polyclonal (Hutterer and Knoblich, 2005, O'Connor-Giles and Skeath, 2003)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: CG31020 BcDNA:RE23355 (FlyBase, 1996-) Source for merge of: spdo CG31020 (O'Connor-Giles and Skeath, 2003)
Additional comments	The "spdo" complementation group corresponds to the CG31020 annotation and not to "tmod" as previously reported in FBrf0102830; molecular lesions in the CG31020 annotation have been identified in 12 spdo alleles, RNAi against CG31020 results in a central nervous system (CNS) phenotype essentially identical to that of spdo mutants and expression of CG31020 throughout the CNS of spdo mutant embryos results in complete to near-complete rescue of the spdo CNS phenotype. (O'Connor-Giles and Skeath, 2003) FlyBase curator comment: FBrf0102830 states that the "spdo" complementation group corresponds to the "tmod, tropomodulin" gene. However, FBrf0162056 shows that this was an error and the spdo complementation group actually corresponds to the CG31020 annotation. (Dye et al., 1998) The "spdo" complementation group corresponds to the CG31020 annotation and not to the CG1539 annotation. It was reported in FBrf0102830 that "spdo" corresponds to the "tmod, tropomodulin" gene (the CG1539 annotation). However, this was subsequently shown to be an error. FBrf0162056 shows that the "spdo" complementation group corresponds instead to the CG31020 annotation. (FlyBase, 1996-) Source for merge of CG31020 BcDNA:RE23355 was a shared cDNA (date:030728). (FlyBase, 1996-)
Other Comments
	New annotation (CG31020) in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003) numb and spdo mediate the asymmetric divisions in the developing heart which result in the production of an odd-expressing pericardial cell and a cardioblast. (Ward and Skeath, 2000) Two EMS induced alleles have been identified in a screen for mutations affecting commissure formation in the CNS of the embryo. (Hummel et al., 1999) Mutations in spdo affect the asymmetric cell division of the embryonic PNS SOPIIb. Rather than a neuron and a glial cell, two neurons are produced. spdo is also required in several other PNS lineages. spdo is epistatic to numb. (Dye et al., 1998) spdo and N act in opposition to numb to distinguish sibling neuron fates in the CNS. (Skeath and Doe, 1998) The numb mutant phenotype is extremely sensitive to the dosage of spdo. (Skeath and Doe, 1998) sanpodo is Korean for mountain grape. (Lee et al., 1995) spdo is required to define glial versus neuronal cell identity. (Lee et al., 1995) Mutation in spdo affects the neuronal lineage, causes transformation of support cells into neurons. (Salzberg et al., 1994) "sanpodo" is Korean for mountain grapes. (Salzberg et al., 1994) spdo is required for PNS development in the embryo. (Salzberg et al., 1994)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_170493 RefSeq (Proteins) • NP_733372 DDBJ / EMBL / GenBank • AAN14235 AAN71331 BI231733 BT001576 Entrez Gene - A searchable database of RefSeq genes. • 318559 UniProtKB/TrEMBL • Q8IGV5 Q8IMI5
Other Crossreferences
Linkouts
	BioGRID - A database of protein and genetic interactions • 76878 DroID - A comprehensive database of gene and protein interactions. • FBgn0260440 DRSC - Results from RNAi screens. • FBgn0260440 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0260440 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0260440 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 318559 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /cytoskel/sanpdo1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 318559 modMine - Data generated by the modENCODE project. • FBgn0260440 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q8IMI5
Synonyms & Secondary IDs ( 7 )
Reported As
Symbol Synonym	0970/02 (Deak et al., 1997) BcDNA:RE23355   CG31020 (Bellen, 2005.1.25, Skeath, 2004, O'Connor-Giles and Skeath, 2003, FlyBase, 1996-, Abrams et al., 2006, Keleman et al., 2009.8.5, Ni et al., 2008, Aerts et al., 2010) spdo (Monastirioti et al., 2010, Wheeler and Crews, 2007, Skeath, 2007.3.20, Karcavich and Doe, 2005, Jafar-Nejad, 2005, Babaoglan et al., 2008, Jafar-Nejad et al., 2005, Tio et al., 2011, Wheeler et al., 2008, Tran and Doe, 2008, O'Connor-Giles and Skeath, 2003, Garces and Thor, 2006, Babaoglan et al., 2009, Rouault et al., 2010, Losada-Pérez et al., 2010, Aerts et al., 2010, Wasbrough et al., 2010, Benhra et al., 2011, Couturier et al., 2012, Ouyang et al., 2011, Japanese National Institute of Genetics, 2012.5.21, Haenfler et al., 2012, Zacharioudaki et al., 2012) Spdo (Benhra et al., 2010, Reed et al., 2008, Giagtzoglou et al., 2012, Song and Lu, 2012, Perdigoto et al., 2011, Moretti et al., 2012)
Name Synonym	sanpodo (Karcavich and Doe, 2005, Albrecht et al., 2006, Roegiers et al., 2005, Tio et al., 2011, Wheeler et al., 2008, O'Connor-Giles and Skeath, 2003, Mok et al., 2005, Hutterer and Knoblich, 2005, Babaoglan et al., 2009, Lacin et al., 2009, Mummery-Widmer et al., 2009, Cohen et al., 2010, Losada-Pérez et al., 2010) Sanpodo (Roegiers et al., 2007, Polo and Di, 2006, Wheeler et al., 2006, Somers and Chia, 2005, Wang et al., 2006, Benhra et al., 2010, Babaoglan et al., 2008, Natarajan and Skeath, 2008, Yamamoto et al., 2008, Roegiers et al., 2005, Coumailleau et al., 2009, Song and Lu, 2012, Tong et al., 2010, Benhra et al., 2011, Moretti et al., 2012)
Secondary FlyBase IDs
	FBgn0010731 FBgn0011716 FBgn0039785 FBgn0039786 FBgn0051020 FBgn0064900
References ( 112 )
Generate a list of	All references relating to this gene ( 112 )
List References by type	Research paper  ( 58 ) Supplementary material  ( 4 ) Review  ( 12 ) Personal communication to FlyBase  ( 10 ) Abstract  ( 24 ) FlyBase analysis  ( 2 ) Stock list  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 13 )
	Couturier et al., 2012, Nat. Cell Biol. 14(2): 131--139 Endocytosis by Numb breaks Notch symmetry at cytokinesis. [FBrf0217937] Giagtzoglou et al., 2012, J. Cell Biol. 196(1): 65--83 dEHBP1 Controls Exocytosis and Recycling of Delta During Asymmetric Divisions. [FBrf0217836] Haenfler et al., 2012, Dev. Biol. 365(1): 219--228 Cortical aPKC kinase activity distinguishes neural stem cells from progenitor cells by ensuring asymmetric segregation of Numb. [FBrf0218950] Lin et al., 2012, PLoS Biol. 10(11): e1001425 Lineage analysis of Drosophila lateral antennal lobe neurons reveals notch-dependent binary temporal fate decisions. [FBrf0219980] Moretti et al., 2012, J. Biol. Chem. 287(35): 29429--29441 The Ubiquitin-specific Protease 12 (USP12) Is a Negative Regulator of Notch Signaling Acting on Notch Receptor Trafficking toward Degradation. [FBrf0219264] Song and Lu, 2012, J. Biol. Chem. 287(21): 17716--17728 Interaction of Notch Signaling Modulator Numb with α-Adaptin Regulates Endocytosis of Notch Pathway Components and Cell Fate Determination of Neural Stem Cells. [FBrf0218342] Ulvklo et al., 2012, Development 139(4): 678--689 Control of neuronal cell fate and number by integration of distinct daughter cell proliferation modes with temporal progression. [FBrf0217281] Zacharioudaki et al., 2012, Development 139(7): 1258--1269 bHLH-O proteins are crucial for Drosophila neuroblast self-renewal and mediate Notch-induced overproliferation. [FBrf0217605] Benhra et al., 2011, Curr. Biol. 21(1): 87--95 AP-1 Controls the Trafficking of Notch and Sanpodo toward E-Cadherin Junctions in Sensory Organ Precursors. [FBrf0212697] Ouyang et al., 2011, Development 138(11): 2185--2196 Dronc caspase exerts a non-apoptotic function to restrain phospho-Numb-induced ectopic neuroblast formation in Drosophila. [FBrf0213702] Perdigoto et al., 2011, Development 138(21): 4585--4595 Distinct levels of Notch activity for commitment and terminal differentiation of stem cells in the adult fly intestine. [FBrf0216374] Song and Lu, 2011, Genes Dev. 25(24): 2644--2658 Regulation of cell growth by Notch signaling and its differential requirement in normal vs. tumor-forming stem cells in Drosophila. [FBrf0217051] Tio et al., 2011, PLoS ONE 6(11): e26879 Asymmetric cell division and notch signaling specify dopaminergic neurons in Drosophila. [FBrf0216651] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...