FlyBase Gene Report: Dmel\rhea

General Information

Symbol

Dmel\rhea

Species

D. melanogaster

Name

rhea

Annotation Symbol

CG6831

Feature Type

protein_coding_gene

FlyBase ID

FBgn0260442

Gene Model Status

Stock Availability

19 publicly available

Gene Snapshot

rhea (rhea) encodes fly talin, a large adaptor protein that is essential for all adhesive functions of integrins. It also has at least one role independent of integrins: repressing shg transcription in follicle cells. [Date last reviewed: 2019-03-14]

Other Summaries

Alliance
Auto summary
Interactive Fly

Also Known As

talin, tendrils

Key Links

Genomic Location

Cytogenetic map

Sequence location

3L:8,545,001..8,574,409 [+]

Recombination map

3-26

RefSeq locus

NT_037436 REGION:8545001..8574409

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (23 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables integrin binding

inferred from physical interaction with FLYBASE:mys; FB:FBgn0004657

(Tanentzapf et al., 2006)

enables protein binding

inferred from physical interaction with FLYBASE:wech; FB:FBgn0259745

(Loer et al., 2008)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables actin filament binding

inferred from electronic annotation with InterPro:IPR006077

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables integrin binding

inferred from biological aspect of ancestor with PANTHER:PTN001072688

(assigned by GO_Central )

(Gaudet et al., 2011)

enables structural constituent of cytoskeleton

inferred from electronic annotation with InterPro:IPR015224

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Biological Process (14 terms)

Terms Based on Experimental Evidence (13 terms)

CV Term

Evidence

References

involved_in apposition of dorsal and ventral imaginal disc-derived wing surfaces

inferred from mutant phenotype

(Prout et al., 1997)

involved_in border follicle cell migration

inferred from mutant phenotype

(Kókai et al., 2012)

involved_in cardiac muscle cell-cardiac muscle cell adhesion

inferred from mutant phenotype

(Bogatan et al., 2015)

involved_in cell adhesion mediated by integrin

inferred from mutant phenotype

(Perkins et al., 2010)

involved_in cell adhesion

inferred from mutant phenotype

(Klapholz et al., 2015)

involved_in germ-band shortening

inferred from mutant phenotype

(Klapholz et al., 2015)

involved_in larval somatic muscle development

inferred from mutant phenotype

(Schnorrer et al., 2010)

involved_in lumen formation, open tracheal system

inferred from high throughput mutant phenotype

(Ghabrial et al., 2011)

involved_in maintenance of epithelial integrity, open tracheal system

inferred from mutant phenotype

(Levi et al., 2006)

involved_in muscle attachment

inferred from mutant phenotype

(Klapholz et al., 2015, Prout et al., 1997)

involved_in negative regulation of transcription, DNA-templated

inferred from mutant phenotype

(Becam et al., 2005)

involved_in sarcomere organization

inferred from mutant phenotype

(Perkins et al., 2010)

involved_in terminal branching, open tracheal system

inferred from high throughput mutant phenotype

(Ghabrial et al., 2011)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in cell adhesion

traceable author statement

(Bokel and Brown, 2002)

involved_in cell-cell adhesion

inferred from biological aspect of ancestor with PANTHER:PTN000463398

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (5 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

located_in focal adhesion

inferred from direct assay

(Delon and Brown, 2009)

located_in plasma membrane

inferred from high throughput direct assay

(Sarov et al., 2016)

located_in Z disc

inferred from high throughput direct assay

(Sarov et al., 2016)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

located_in cytoskeleton

inferred from electronic annotation with InterPro:IPR000299

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

located_in focal adhesion

inferred from biological aspect of ancestor with PANTHER:PTN001072688

(assigned by GO_Central )

(Gaudet et al., 2011)

inferred from sequence or structural similarity

(Hynes and Zhao, 2000)

located_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN000463398

(assigned by GO_Central )

(Gaudet et al., 2011)

located_in ruffle

inferred from electronic annotation with InterPro:IPR015224

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Gene Group (FlyBase)

Protein Family (UniProt)

-

Protein Signatures (InterPro)

Summaries

Summary (Interactive Fly)

(preferred name: Rhea) essential for integrin function - crosslinks extracellular matrix-linked integrins to the cytoskeleton - represses E-cadherin Shotgun transcription in follicle cells independently of integrins

Interactive Fly

Gene Model and Products

Number of Transcripts

8

Number of Unique Polypeptides

5

Please see the JBrowse view of Dmel\rhea for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.46

Alternative translation stop created by use of multiphasic reading frames within coding region.

Sequence Ontology: Class of Gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0305210

9584

2836

FBtr0305211

7261

2169

FBtr0305212

7373

2169

FBtr0333105

9937

2836

FBtr0333106

9409

2836

FBtr0333107

9521

2815

FBtr0333108

9265

2689

FBtr0333109

9722

513

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

FBpp0293740

307.0

2836

6.14

FBpp0293741

235.3

2169

5.87

FBpp0293742

235.3

2169

5.87

FBpp0305317

307.0

2836

6.14

FBpp0305318

307.0

2836

6.14

FBpp0305319

304.7

2815

6.20

FBpp0305320

291.5

2689

5.95

FBpp0305321

58.8

513

7.04

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

2836 aa isoforms: rhea-PB, rhea-PE, rhea-PF

2169 aa isoforms: rhea-PC, rhea-PD

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\rhea using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 16

ventral nervous system

(Brown et al., 2002)

embryonic/larval midgut primordium

(Brown et al., 2002)

muscle attachment site

(Brown et al., 2002)

embryonic stage 6

organism | ubiquitous

(Brown et al., 2002)

embryonic stage 12

ventral nervous system

(Brown et al., 2002)

embryonic stage 13 -- 16

larval midline neuron

(Kearney et al., 2004)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 6

• cytoplasm

(Brown et al., 2002)

embryonic stage 16

muscle attachment site

• plasma membrane & adherens junction

(Brown et al., 2002)

third instar larval stage

wing disc | restricted

(Brown et al., 2002)

embryonic stage 12

anterior midgut primordium & posterior midgut primordium

• plasma membrane

(Brown et al., 2002)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 5 of adulthood & male -- day 7 of adulthood & male

(Wasbrough et al., 2010)

day 7 of adulthood -- day 49 of adulthood

(Cammarato et al., 2011)

• membrane

(Aradska et al., 2015)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

(Wheeler and Hynes, 2001)

Additional Descriptive Data

The rhea protein was detected in the cytoplasm of early cellularized embryos. Subsequently, rhea protein concentrated at the membrane of migrating midgut primordial cells and then at muscle attachment sites, specifically at hemi-adherens junctions. In wing imaginal discs the rhea protein localized to focal adhesion like structures at the basal surface.

(Brown et al., 2002)

Marker for

(Johnson et al., 2013)

Subcellular Localization

CV Term

Evidence

References

located_in focal adhesion

inferred from direct assay

(Delon and Brown, 2009)

located_in plasma membrane

inferred from high throughput direct assay

(Sarov et al., 2016)

located_in Z disc

inferred from high throughput direct assay

(Sarov et al., 2016)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\rhea in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\rhea

View exonic expression by tissue for Dmel\rhea

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0260442

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6831

Flygut - An atlas of the Drosophila adult midgut

FBgn0260442

Images

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 69 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 45 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of rhea

Transgenic constructs containing regulatory region of rhea

Deletions and Duplications ( 5 )

Disrupted in

(Ghabrial et al., 2011, Brown et al., 2002)

(Prout et al., 1997)

Not disrupted in

(Levi et al., 2006)

(Levi et al., 2006)

(Levi et al., 2006)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Df(3L)79a

lethal, with Scer\GAL4^elav.PLu

rhea^GD12050

lethal, with Scer\GAL4^Mef2.PR

rhea^GD12050

lethal, with Scer\GAL4^pnr-MD237

rhea^GD12050

lethal, with Scer\GAL4^tin.CΔ4

rhea^GD12050

lethal (with rhea^6-66)

lethal (with rhea^13-8)

lethal (with rhea^14-69)

lethal (with rhea^15-39)

lethal | pupal stage, with Scer\GAL4^pnr-MD237

rhea^GD12050

lethal - all die before end of embryonic stage

lethal - all die before end of embryonic stage | recessive

rhea¹

lethal - all die during embryonic stage

rhea^13-8

partially lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

rhea^GD12050

partially lethal - majority die, with Scer\GAL4^Hand.PU

rhea^GD12050

rhea^MI00296

Other Phenotypes

Allele

cell adhesion defective, with Df(3L)79a

rhea^Δhead.GFP

cell adhesion defective | embryonic stage 17

rhea^13-8

cell adhesion defective | recessive

decreased cell number | adult stage, with Scer\GAL4^NP5130

decreased cell number | adult stage | somatic clone

decreased cell number | third instar larval stage, with Scer\GAL4^repo

rhea^HMS00799

increased cell size | larval stage, with Scer\GAL4^hth-GETDB

rhea^GD12050

majority die during P-stage, with Scer\GAL4^Hand.PU

rhea^GD12050

neuroanatomy defective | third instar larval stage, with Scer\GAL4^repo

short lived, with Scer\GAL4^Hand.PU

rhea^GD12050

size defective | adult stage | somatic clone

some die during embryonic stage

some die during embryonic stage | recessive

rhea¹

some die during pupal stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

rhea^GD12050

visible | somatic clone

rhea¹

Phenotype manifest in

Allele

actin filament & follicle cell | somatic clone

rhea^unspecified

adult heart muscle, with Scer\GAL4^Hand.PU

rhea^GD12050

adult posterior midgut epithelium, with Scer\GAL4^NP5130

adult posterior midgut epithelium | somatic clone

antennal disc | larval stage, with Scer\GAL4^hth-GETDB

rhea^GD12050

border follicle cell, with Scer\GAL4^slbo.2.6

rhea^NIG.6831R

cardial cell, with Scer\GAL4^Hand.PU

rhea^GD12050

cardial cell | larval stage, with Scer\GAL4^Hand.PU

rhea^GD12050

egg chamber | conditional, with Scer\GAL4^hs.PU

rhea^VDRC.cUa

egg chamber | somatic clone

rhea^unspecified

embryo | dorsal closure stage

rhea¹⁷

embryo | dorsal closure stage, with Df(3L)79a

rhea^{E1777A.Ubi-p63E.EGFP}

embryo | dorsal closure stage (with Df(3L)79a)

rhea¹⁷

embryo | embryonic stage 12

rhea¹⁷

embryo | embryonic stage 12 (with Df(3L)79a)

rhea¹⁷

embryonic/larval fat body branch | somatic clone

rhea^13-8

embryonic/larval heart | larval stage, with Scer\GAL4^Hand.PU

rhea^GD12050

embryonic/larval heart | third instar larval stage, with Scer\GAL4^Hand.PU

rhea^HM05161

embryonic/larval heart visceral muscle | third instar larval stage, with Scer\GAL4^Hand.PU

rhea^HM05161

embryonic/larval midgut primordium

rhea²

embryonic/larval optic stalk | third instar larval stage, with Scer\GAL4^repo

embryonic/larval somatic muscle

rhea¹

embryonic/larval somatic muscle, with Scer\GAL4^Mef2.PR

rhea^GD12050

embryonic/larval tracheal system

embryonic proventriculus

rhea²

embryonic somatic muscle | embryonic stage 17

rhea^13-8

embryonic somatic muscle | embryonic stage 17, with Scer\GAL4^Mef2.PU, rhea^13-8

rhea^{T150A.T152A.UAS.Tag:FLAG}

embryonic somatic muscle | embryonic stage 17, with Scer\GAL4^Mef2.PU, rhea^{T150A.T152A.UAS.Tag:FLAG}

rhea^13-8

eye | somatic clone

eye disc | third instar larval stage, with Scer\GAL4^repo

eye photoreceptor cell | third instar larval stage, with Scer\GAL4^repo

gastric caecum primordium

rhea²

germ band | rescuable maternal effect

rhea²

glial cell | third instar larval stage, with Scer\GAL4^repo

rhea^HMS00799

intestinal stem cell of posterior adult midgut epithelium, with Scer\GAL4^NP5130

muscle attachment site

muscle attachment site, with Df(3L)79a

muscle attachment site | embryonic stage | germline clone, with Df(3L)79a

rhea^{R367A.Ubi-p63E}

muscle attachment site | rescuable maternal effect

rhea²

muscle cell & actin filament & embryo

rhea^unspecified

muscle cell & focal adhesion & embryo

rhea^unspecified

muscle cell | embryonic stage

rhea^unspecified

muscle tendon junction

rhea¹⁷

myofibril, with Scer\GAL4^Hand.PU

rhea^GD12050

oocyte | cell non-autonomous | somatic clone

rhea²

presumptive embryonic/larval muscle system | embryonic stage | germline clone, with Df(3L)79a

rhea^{R367A.Ubi-p63E}

sarcomere, with Scer\GAL4^Mef2.PR

rhea^GD12050

tendon cell & basal lamina & embryo

rhea^unspecified

tendon cell & focal adhesion & embryo

rhea^unspecified

tendon cell & microtubule & embryo

rhea^unspecified

tendon cell | embryonic stage

rhea^unspecified

terminal tracheal cell

terminal tracheal cell | somatic clone

tracheal system

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (4)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

14 of 15

Yes

Yes

12 of 15

No

Yes

3 of 15

No

Yes

2 of 15

No

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (3)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

14 of 15

Yes

Yes

12 of 15

No

Yes

3 of 15

No

Yes

Rattus norvegicus (Norway rat) (3)

11 of 13

Yes

Yes

7 of 13

No

Yes

3 of 13

No

Yes

Xenopus tropicalis (Western clawed frog) (3)

9 of 12

Yes

Yes

3 of 12

No

Yes

1 of 12

No

Yes

Danio rerio (Zebrafish) (4)

11 of 15

Yes

Yes

10 of 15

No

Yes

3 of 15

No

Yes

2 of 15

No

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

14 of 15

Yes

Yes

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG0919003J )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Dsuz\DS10_00003750

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Drosophila persimilis

Drosophila willistoni

Drosophila virilis

Drosophila mojavensis

Drosophila grimshawi

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915002G )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Glossina morsitans

Tsetse fly

Gmor\GMOY004670

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_07751

Mayetiola destructor

Hessian fly

Mdes\Mdes014722

Mayetiola destructor

Hessian fly

Mdes\Mdes000258

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL006222

Anopheles darlingi

American malaria mosquito

Adar\ADAC002250

Anopheles darlingi

American malaria mosquito

Adar\ADAC002257

Anopheles gambiae

Malaria mosquito

Agam\AGAP007474

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ011390

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W02DE )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA001370

Bombyx mori

Silkmoth

Bmor\BGIBMGA001371

Bombyx mori

Silkmoth

Bmor\BGIBMGA001372

Danaus plexippus

Monarch butterfly

Dple\DPOGS211677-PA

Danaus plexippus

Monarch butterfly

Dple\DPOGS211678-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL015881

Heliconius melpomene

Postman butterfly

Hmel\HMEL015879

Apis florea

Little honeybee

Aflo\2408700516

Apis mellifera

Western honey bee

Apis mellifera

Western honey bee

Bombus impatiens

Common eastern bumble bee

Bombus impatiens

Common eastern bumble bee

Bombus terrestris

Buff-tailed bumblebee

Linepithema humile

Argentine ant

Linepithema humile

Argentine ant

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:001868

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG002134

Dendroctonus ponderosae

Mountain pine beetle

Tribolium castaneum

Red flour beetle

Pediculus humanus

Human body louse

Phum\PHUM574140

Rhodnius prolixus

Kissing bug

Rpro\RPRC007435

Cimex lectularius

Bed bug

Clec\CLEC009792

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI003888

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI000925

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_01319

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X02AV )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR007090

Ixodes scapularis

Black-legged tick

Isca\ISCW023338

Stegodyphus mimosarum

African social velvet spider

Smim\KFM78418.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur04g06860

Tetranychus urticae

Two-spotted spider mite

Turt\tetur04g06830

Tetranychus urticae

Two-spotted spider mite

Turt\tetur04g06840

Tetranychus urticae

Two-spotted spider mite

Turt\tetur04g07040

Tetranychus urticae

Two-spotted spider mite

Turt\tetur10g01300

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_41019

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_32232

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0644 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_006921gn

Strongylocentrotus purpuratus

Purple sea urchin

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000009522

Gallus gallus

Domestic chicken

Gallus gallus

Domestic chicken

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (0)

No records found.

Human Disease Associations

FlyBase Human Disease Model Reports

cardiac dysfunction (postulated), talin-related

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

14 of 15

12 of 15

TLNRD1; talin rod domain containing 1

3 of 15

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

enzymatic study, western blot, anti bait coimmunoprecipitation

(Kókai et al., 2012)

pull down, peptide massfingerprinting

(Gillingham et al., 2014)

protein kinase assay, western blot

(Katzemich et al., 2019)

phenotype-based detection assay, fluorescence microscopy, inferred by author

(Maartens et al., 2016)

phenotype-based detection assay, fluorescence microscopy, inferred by author

(Tanentzapf et al., 2006)

pull down, western blot, anti tag coimmunoprecipitation, anti tag western blot

(Loer et al., 2008)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

(Kókai et al., 2012)

suppressible

(Tanentzapf and Brown, 2006)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

(Kókai et al., 2012)

suppressible

(Kókai et al., 2012)

enhanceable

(Pronovost et al., 2013)

suppressible

(Pronovost et al., 2013)

enhanceable

(Prout et al., 1997)

enhanceable

(MacMullin and Jacobs, 2006)

suppressible

(Katzemich et al., 2019)

suppressible

(Issigonis et al., 2009)

suppressible

(Maartens et al., 2016)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

64387

DroID - A comprehensive database of gene and protein interactions.

FBgn0260442

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

38978

MIST (genetic) - An integrated Molecular Interaction Database

rhea

MIST (protein-protein) - An integrated Molecular Interaction Database

rhea

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

3L

Recombination map

3-26

Cytogenetic map

Sequence location

3L:8,545,001..8,574,409 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

66D6-66D7

Limits computationally determined from genome sequence between P{lacW}foi^j8E8 and P{PZ}SrpRβ^rK561&P{lacW}l(3)j5B6^j5B6

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

rhea is not located at 75A-75C, but is elsewhere on 3L.

(Fristrom, 1997.8.6)

Experimentally Determined Recombination Data

Location

3-26

(FlyBase, 2016)

3-20.1

(Comeron et al., 2012)

3-

(Ghabrial et al., 2011, Prout et al., 1997)

Left of (cM)

h

(Ghabrial et al., 2011)

Right of (cM)

ru

(Ghabrial et al., 2011)

Notes

Located on 3L.

(Prout et al., 1997)

Stocks and Reagents

Stocks (19)

28950

y¹ v¹; P{TRiP.HM05161}attP2

82654

y¹ sc^* v¹ sev²¹; P{TKO.GS04131}attP40

83596

y¹ sc^* v¹ sev²¹; P{TOE.GS05257}attP40

32999

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00799}attP2

33913

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00856}attP2

30955

y¹ w^*; Mi{MIC}rhea^MI00296 lncRNA:CR43910^MI00296

51194

y¹ w^*; Mi{MIC}rhea^MI00738/TM3, Sb¹ Ser¹

v40399

w¹¹¹⁸; P{GD12050}v40399

v318486

PBac{fTRG00587.sfGFP-TVPTBF}VK00033

v318475

PBac{fTRG00731.sfGFP-TVPTBF}VK00033

v40400

w¹¹¹⁸; P{GD12050}v40400/CyO

v318423

PBac{fTRG10236.sfGFP-nlsTTSTF}VK00033

More stocks available...

Genomic Clones (38)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (86)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

SD07967

Other clones

MIP14644

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0260442

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0260442

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

38978

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Katzemich et al., 2019, Tanentzapf et al., 2006)

monoclonal, polyclonal

(Brown et al., 2002)

Commercially Available Antibodies

Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research

Talin_A22A

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: CG6831 Talin

(Brody et al., 2000)

Source for database merge of

Source for merge of: Talin rhea

(Brown et al., 2002)

Additional comments

mir-190 is located within an intron of rhea.

(Stark et al., 2007, Stark et al., 2007)

Other Comments

rhea is required continuously during heart growth to maintain the one-to-one apposition of myofibril ends between cardiomyocytes. Retracted myofibrils cannot regenerate appositions to adjacent cells if normal rhea expression is subsequently restored.

(Bogatan et al., 2015)

Distinct combinations of talin (rhea) domains are required for each of three different integrin functions during development. The talin IBS2 domain is separated from integrins in the muscles but not at wing adhesion sites, and this separation partly requires myosin activity and Vinc.

(Klapholz et al., 2015)

rhea is required for maintenance of tracheal terminal branches and luminal organization.

(Levi et al., 2006)

S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.

(Stroschein-Stevenson et al., 2006)

rhea is required to specifically inhibit transcription of shg in the somatic follicle cells.

(Becam et al., 2005)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in S2R⁺ cells: cells become round and detached. Kc167 cells are unaffected.

(Kiger et al., 2003)

dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation.

(Rogers et al., 2003)

Identification: Genetic screen for autosomal mutations that produce blisters in somatic wing clones.

(Prout et al., 1997)

Mutations affect myo-epidermal junctions or muscle function in embryos.

(Prout et al., 1997)

Identification: Genetic screen for autosomal mutations that produce blisters in somatic wing clones. 2 alleles of rhea have been isolated.

(Prout et al., 1997)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 77 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

38978

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0260442

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6831

Flygut - An atlas of the Drosophila adult midgut

FBgn0260442

FlyMine - An integrated database for Drosophila genomics

FBgn0260442

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

38978

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC033529

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG6831

modMine - A data warehouse for the modENCODE project

FBgn0260442

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q9VSL8

Linkouts

BioGRID - A database of protein and genetic interactions.

64387

DroID - A comprehensive database of gene and protein interactions.

FBgn0260442

DRSC - Results frm RNAi screens

FBgn0260442

Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research

Talin_A22A

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0260442

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

38978

MIST (genetic) - An integrated Molecular Interaction Database

rhea

MIST (protein-protein) - An integrated Molecular Interaction Database

rhea

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

CG6831

(Balakrishnan et al., 2015, Hosono et al., 2015, Christis and Munro, 2012, Yano et al., 2012, Cammarato et al., 2011, Ni et al., 2010.12.1, Krejcí et al., 2009, Perkins et al., 2009, Carrera et al., 2008, Montana and Littleton, 2006, Rogers et al., 2003, Brody et al., 2002, Brown et al., 2002, Goldstein and Gunawardena, 2000)

CT21159

(Hynes and Zhao, 2000)

Rhea

(Fujisawa et al., 2020, Proag et al., 2019, Wang et al., 2014, Venken et al., 2011, Dibya et al., 2009, Kim et al., 2005)

Tln

(Senetar and McCann, 2005)

rhea

(Bajpai et al., 2020, Chougule et al., 2020, Gilbert et al., 2020, Hunter et al., 2020, Jammrath et al., 2020, Katzemich et al., 2019, Park et al., 2019, Yee et al., 2019, Camp et al., 2018, Cho et al., 2018, Gusareva et al., 2018, Klapholz and Brown, 2017, Rohde et al., 2017, Transgenic RNAi Project members, 2017-, Vanderploeg and Jacobs, 2017, De Lella Ezcurra et al., 2016, Sarov et al., 2016, Álvarez-Fernández et al., 2015, Gene Disruption Project members, 2015-, Klapholz et al., 2015, Maartens and Brown, 2015, Morozova et al., 2015, Ashwal-Fluss et al., 2014, Chen et al., 2014, Ellis et al., 2014, Gómez-Lamarca et al., 2014, Gupta et al., 2014, Perkins and Tanentzapf, 2014, Bharadwaj et al., 2013, Comber et al., 2013, Kwon et al., 2013, Lin et al., 2013, Moreira et al., 2013, Zhang et al., 2013, Bouaouina et al., 2012, Gates, 2012, Japanese National Institute of Genetics, 2012.5.21, Vanderploeg et al., 2012, Wu et al., 2012, Yano et al., 2012, Ellis et al., 2011, Friedman et al., 2011, Knox et al., 2011, Venken et al., 2011, Zervas et al., 2011, Carmon et al., 2010, Deng et al., 2010, Franco-Cea et al., 2010, Perkins et al., 2010, Schnorrer et al., 2010, Wasbrough et al., 2010, Issigonis et al., 2009, Krejcí et al., 2009, Carrera et al., 2008, Fernandez-Minan et al., 2008, Helsten et al., 2008, Loer et al., 2008, Beltran et al., 2007, Dietzl et al., 2007, Dominguez-Gimenez et al., 2007, Fernández-Miñán et al., 2007, Jani and Schock, 2007, Stark et al., 2007, Stark et al., 2007, Tanentzapf et al., 2007, Levi et al., 2006, Levi et al., 2006, Lo, 2006, MacMullin and Jacobs, 2006, Stroschein-Stevenson et al., 2006, Tanentzapf and Brown, 2006)

tendrils

(Ghabrial et al., 2011)

Name Synonyms

rhea

(Cammarato et al., 2011, Carmon et al., 2010, Krejcí et al., 2009, Beckstead et al., 2005, Prout et al., 1997)

tendrils

(Ghabrial et al., 2011, Levi et al., 2005)

Secondary FlyBase IDs

FBgn0035910
FBgn0020254

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (224)