General Information
Symbol
Dmel\rhea
Species
D. melanogaster
Name
rhea
Annotation Symbol
CG6831
Feature Type
FlyBase ID
FBgn0260442
Gene Model Status
Stock Availability
Gene Snapshot
Rhea is fly talin, a large adaptor protein that is essential for all adhesive functions of integrins. It also has at least one role independent of integrins: repressing E-cadherin shg transcription in follicle cells. [Date last reviewed: 2017-07-20]
Also Known As
talin
Genomic Location
Cytogenetic map
Sequence location
3L:8,545,001..8,574,409 [+]
Recombination map
3-26
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
GO Summary Ribbons
Families, Domains and Molecular Function
Gene Group Membership (FlyBase)
Protein Family (UniProt, Sequence Similarities)
-
Molecular Function (see GO section for details)
Summaries
Gene Model and Products
Number of Transcripts
8
Number of Unique Polypeptides
5

Please see the GBrowse view of Dmel\rhea or the JBrowse view of Dmel\rhea for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Gene model reviewed during 5.46
Alternative translation stop created by use of multiphasic reading frames within coding region.
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0305210
9584
2836
FBtr0305211
7261
2169
FBtr0305212
7373
2169
FBtr0333105
9937
2836
FBtr0333106
9409
2836
FBtr0333107
9521
2815
FBtr0333108
9265
2689
FBtr0333109
9722
513
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0293740
307.0
2836
6.14
FBpp0293741
235.3
2169
5.87
FBpp0293742
235.3
2169
5.87
FBpp0305317
307.0
2836
6.14
FBpp0305318
307.0
2836
6.14
FBpp0305319
304.7
2815
6.20
FBpp0305320
291.5
2689
5.95
FBpp0305321
58.8
513
7.04
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

2836 aa isoforms: rhea-PB, rhea-PE, rhea-PF
2169 aa isoforms: rhea-PC, rhea-PD
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\rhea using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (22 terms)
Molecular Function (5 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
inferred from physical interaction with FLYBASE:mys; FB:FBgn0004657
inferred from physical interaction with FLYBASE:wech; FB:FBgn0259745
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from sequence or structural similarity
inferred from electronic annotation with InterPro:IPR006077
(assigned by InterPro )
inferred from electronic annotation with InterPro:IPR015224
(assigned by InterPro )
Biological Process (14 terms)
Terms Based on Experimental Evidence (13 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
traceable author statement
inferred from electronic annotation with InterPro:IPR015224
(assigned by InterPro )
inferred from sequence or structural similarity
Cellular Component (3 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from direct assay
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR000299
(assigned by InterPro )
inferred from sequence or structural similarity
inferred from electronic annotation with InterPro:IPR015224
(assigned by InterPro )
Expression Data
Transcript Expression
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
The rhea protein was detected in the cytoplasm of early cellularized embryos. Subsequently, rhea protein concentrated at the membrane of migrating midgut primordial cells and then at muscle attachment sites, specifically at hemi-adherens junctions. In wing imaginal discs the rhea protein localized to focal adhesion like structures at the basal surface.
Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\rhea in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, Transgenic Constructs and Phenotypes
Classical and Insertion Alleles ( 56 )
For All Classical and Insertion Alleles Show
 
Allele of rhea
Class
Mutagen
Associated Insertion
Stocks
Known lesion
    0
    Yes
    Other relevant insertions
    insertion of mobile activating element
    miscellaneous insertions
    Transgenic Constructs ( 38 )
    For All Alleles Carried on Transgenic Constructs Show
    Transgenic constructs containing/affecting coding region of rhea
    Allele of rhea
    Mutagen
    Associated Transgenic Construct
    Stocks
    Transgenic constructs containing regulatory region of rhea
    vital-reporter construct
    Name
    Expression Data
    Deletions and Duplications ( 5 )
    Summary of Phenotypes
    For more details about a specific phenotype click on the relevant allele symbol.
    Lethality
    Allele
    Other Phenotypes
    Allele
    Phenotype manifest in
    Allele
    actin filament & follicle cell | somatic clone
    muscle cell & actin filament & embryo
    muscle cell & focal adhesion & embryo
    tendon cell & basal lamina & embryo
    tendon cell & focal adhesion & embryo
    tendon cell & microtubule & embryo
    Orthologs
    Human Orthologs (via DIOPT v7.1)
    Homo sapiens (Human) (3)
    Species\Gene Symbol
    Score
    Best Score
    Best Reverse Score
    Alignment
    Complementation?
    Transgene?
    14 of 15
    Yes
    Yes
    12 of 15
    No
    Yes
    2 of 15
    No
    Yes
    Model Organism Orthologs (via DIOPT v7.1)
    Mus musculus (laboratory mouse) (3)
    Species\Gene Symbol
    Score
    Best Score
    Best Reverse Score
    Alignment
    Complementation?
    Transgene?
    13 of 15
    Yes
    Yes
    11 of 15
    No
    Yes
    2 of 15
    No
    Yes
    Rattus norvegicus (Norway rat) (3)
    9 of 13
    Yes
    Yes
    7 of 13
    No
    Yes
    2 of 13
    No
    Yes
    Xenopus tropicalis (Western clawed frog) (2)
    7 of 12
    Yes
    Yes
    1 of 12
    No
    Yes
    Danio rerio (Zebrafish) (4)
    11 of 15
    Yes
    Yes
    10 of 15
    No
    Yes
    2 of 15
    No
    Yes
    1 of 15
    No
    Yes
    Caenorhabditis elegans (Nematode, roundworm) (1)
    14 of 15
    Yes
    Yes
    Arabidopsis thaliana (thale-cress) (4)
    1 of 9
    Yes
    Yes
    1 of 9
    Yes
    Yes
    1 of 9
    Yes
    Yes
    1 of 9
    Yes
    Yes
    Saccharomyces cerevisiae (Brewer's yeast) (1)
    1 of 15
    Yes
    No
    Schizosaccharomyces pombe (Fission yeast) (0)
    No orthologs reported.
    Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG0919003J )
    Organism
    Common Name
    Gene
    AAA Syntenic Ortholog
    Multiple Dmel Genes in this Orthologous Group
    Drosophila melanogaster
    fruit fly
    Drosophila suzukii
    Spotted wing Drosophila
    Drosophila simulans
    Drosophila sechellia
    Drosophila erecta
    Drosophila yakuba
    Drosophila ananassae
    Drosophila pseudoobscura pseudoobscura
    Drosophila persimilis
    Drosophila willistoni
    Drosophila virilis
    Drosophila mojavensis
    Drosophila grimshawi
    Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915002G )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Musca domestica
    House fly
    Glossina morsitans
    Tsetse fly
    Lucilia cuprina
    Australian sheep blowfly
    Mayetiola destructor
    Hessian fly
    Mayetiola destructor
    Hessian fly
    Aedes aegypti
    Yellow fever mosquito
    Anopheles darlingi
    American malaria mosquito
    Anopheles darlingi
    American malaria mosquito
    Anopheles gambiae
    Malaria mosquito
    Culex quinquefasciatus
    Southern house mosquito
    Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W02DE )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Bombyx mori
    Silkmoth
    Bombyx mori
    Silkmoth
    Bombyx mori
    Silkmoth
    Danaus plexippus
    Monarch butterfly
    Danaus plexippus
    Monarch butterfly
    Heliconius melpomene
    Postman butterfly
    Heliconius melpomene
    Postman butterfly
    Apis florea
    Little honeybee
    Apis mellifera
    Western honey bee
    Apis mellifera
    Western honey bee
    Bombus impatiens
    Common eastern bumble bee
    Bombus impatiens
    Common eastern bumble bee
    Bombus terrestris
    Buff-tailed bumblebee
    Linepithema humile
    Argentine ant
    Linepithema humile
    Argentine ant
    Megachile rotundata
    Alfalfa leafcutting bee
    Nasonia vitripennis
    Parasitic wasp
    Dendroctonus ponderosae
    Mountain pine beetle
    Tribolium castaneum
    Red flour beetle
    Pediculus humanus
    Human body louse
    Rhodnius prolixus
    Kissing bug
    Cimex lectularius
    Bed bug
    Acyrthosiphon pisum
    Pea aphid
    Acyrthosiphon pisum
    Pea aphid
    Zootermopsis nevadensis
    Nevada dampwood termite
    Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X02AV )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Strigamia maritima
    European centipede
    Ixodes scapularis
    Black-legged tick
    Stegodyphus mimosarum
    African social velvet spider
    Tetranychus urticae
    Two-spotted spider mite
    Tetranychus urticae
    Two-spotted spider mite
    Tetranychus urticae
    Two-spotted spider mite
    Tetranychus urticae
    Two-spotted spider mite
    Tetranychus urticae
    Two-spotted spider mite
    Daphnia pulex
    Water flea
    Daphnia pulex
    Water flea
    Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0644 )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Strongylocentrotus purpuratus
    Purple sea urchin
    Strongylocentrotus purpuratus
    Purple sea urchin
    Strongylocentrotus purpuratus
    Purple sea urchin
    Ciona intestinalis
    Vase tunicate
    Gallus gallus
    Domestic chicken
    Gallus gallus
    Domestic chicken
    Human Disease Model Data
    FlyBase Human Disease Model Reports
      Alleles Reported to Model Human Disease (Disease Ontology)
      Download
      Models ( 0 )
      Allele
      Disease
      Evidence
      References
      Interactions ( 0 )
      Allele
      Disease
      Interaction
      References
      Comments ( 0 )
       
      Human Orthologs (via DIOPT v7.1)
      Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
      Homo sapiens (Human)
      Gene name
      Score
      OMIM
      OMIM Phenotype
      Complementation?
      Transgene?
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      esyN Network Diagram
      Show neighbor-neighbor interactions:
      Select Layout:
      Legend:
      Protein
      RNA
      Selected Interactor(s)
      Interactions Browser

      Please look at the Interaction Group reports for full details of the physical interactions
      protein-protein
      Interacting group
      Assay
      References
      Summary of Genetic Interactions
      esyN Network Diagram
      esyN Network Key:
      Suppression
      Enhancement

      Please look at the allele data for full details of the genetic interactions
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      External Data
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      Pathways
      Gene Group - Pathway Membership (FlyBase)
      External Data
      Linkouts
      SignaLink - A signaling pathway resource with multi-layered regulatory networks.
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      3L
      Recombination map
      3-26
      Cytogenetic map
      Sequence location
      3L:8,545,001..8,574,409 [+]
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      66D6-66D7
      Limits computationally determined from genome sequence between P{lacW}foij8E8 and P{PZ}SrpRβrK561&P{lacW}l(3)j5B6j5B6
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      rhea is not located at 75A-75C, but is elsewhere on 3L.
      Experimentally Determined Recombination Data
      Left of (cM)
      Right of (cM)
      Notes
      Located on 3L.
      Stocks and Reagents
      Stocks (17)
      Genomic Clones (38)
      cDNA Clones (86)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequences
      BDGP DGC clones
      Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      Antibody Information
      Laboratory Generated Antibodies
       
      monoclonal, polyclonal
      Commercially Available Antibodies
       
      Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
      Other Information
      Relationship to Other Genes
      Source for database identify of
      Source for identity of: CG6831 Talin
      Source for database merge of
      Source for merge of: Talin rhea
      Additional comments
      mir-190 is located within an intron of rhea.
      Other Comments
      rhea is required continuously during heart growth to maintain the one-to-one apposition of myofibril ends between cardiomyocytes. Retracted myofibrils cannot regenerate appositions to adjacent cells if normal rhea expression is subsequently restored.
      Distinct combinations of talin (rhea) domains are required for each of three different integrin functions during development. The talin IBS2 domain is separated from integrins in the muscles but not at wing adhesion sites, and this separation partly requires myosin activity and Vinc.
      rhea is required for maintenance of tracheal terminal branches and luminal organization.
      S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.
      rhea is required to specifically inhibit transcription of shg in the somatic follicle cells.
      dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
      RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in S2R+ cells: cells become round and detached. Kc167 cells are unaffected.
      dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation.
      Identification: Genetic screen for autosomal mutations that produce blisters in somatic wing clones.
      Mutations affect myo-epidermal junctions or muscle function in embryos.
      Identification: Genetic screen for autosomal mutations that produce blisters in somatic wing clones. 2 alleles of rhea have been isolated.
      Origin and Etymology
      Discoverer
      Etymology
      Identification
      External Crossreferences and Linkouts ( 77 )
      Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center cDNA clones
      DroID - A comprehensive database of gene and protein interactions.
      DRSC - Results frm RNAi screens
      Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
      FLIGHT - Cell culture data for RNAi and other high-throughput technologies
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Flygut - An atlas of the Drosophila adult midgut
      FlyMine - An integrated database for Drosophila genomics
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
      Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      KEGG Genes - Molecular building blocks of life in the genomic space.
      modMine - A data warehouse for the modENCODE project
      SignaLink - A signaling pathway resource with multi-layered regulatory networks.
      Synonyms and Secondary IDs (8)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
      • FBgn0035910
      • FBgn0020254
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      References (200)