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Gene Dmel\γTub23C

General Information
Symbol	Dmel\γTub23C	Species	D. melanogaster
Name	γ-Tubulin at 23C	Annotation symbol	CG3157
Feature type	protein_coding_gene	FlyBase ID	FBgn0260639
Gene Model Status	Current	Stock availability	10 publicly available
Also Known As	γTub, γ-tub, gammaTub23C, γ-Tub23C, γ-tubulin, GTU88*
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	23C1-23C4	Sequence location	2L:2,972,892..2,974,862 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene γ-Tubulin at 23C is referred to in FlyBase by the symbol Dmel\γTub23C (CG3157, FBgn0260639). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: GTP binding; guanyl nucleotide binding; microtubule minus-end binding. There is experimental evidence that it is involved in the biological process: centrosome organization; microtubule nucleation; mitotic sister chromatid separation; mitosis; mitotic spindle organization; centriole-centriole cohesion; centrosome duplication; regulation of cell cycle. 23 alleles are reported. The phenotypes of these alleles are annotated with: organ system subdivision; adult segment; organ system; thoracic segment; organelle; spindle; adult; imaginal precursor; external compound sense organ; intracellular organelle part. It has one annotated transcript and one annotated polypeptide. Protein features are: Gamma tubulin; Tubulin; Tubulin, C-terminal; Tubulin, conserved site; Tubulin/FtsZ, 2-layer sandwich domain; Tubulin/FtsZ, C-terminal; Tubulin/FtsZ, GTPase domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, larval trachea, adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1626125_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of gammaTub23C. Gene sequence location is 2L:2972892..2974862.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 23C1-23C4   Left limit from inclusion within Df(2L)JS17 (FBrf0125088) Right limit from inclusion within Df(2L)JS17 (FBrf0125088); Limits computationally determined from genome sequence between P{PZ}lilli00632 and P{PZ}toc01361
Experimentally Determined Cytological Location
Cytogenetic map Notes References 23C1-23C4   (determined by in situ hybridisation)   (Sunkel et al., 1995) 23C-23D   (determined by in situ hybridisation)   (Zheng et al., 1991)
Experimentally Determined Recombination Data
Location
Left of (cM)	Rrp1 (Huang et al., 1998) P{EPgy2}CG9643EY07345 dp (Vázquez et al., 2008)
Right of (cM)	al P{EP}Rrp1EP1020 (Vázquez et al., 2008)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\γTub23C for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) γTub23C-RA FBtr0077641  NM_057456   1853   475
Additional Transcript Data & Comments
Reported size (kB)	1.875 (longest cDNA) (Zheng et al., 1991)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein gammaTub23C-PA   FBpp0077326   53.3   475   5.78     NP_476804   AAF51174
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Tubulin, conserved site (IPR017975) Tubulin/FtsZ, 2-layer sandwich domain (IPR018316) Tubulin (IPR000217) Gamma tubulin (IPR002454) Tubulin/FtsZ, GTPase domain (IPR003008) Tubulin/FtsZ, C-terminal (IPR008280) Tubulin, C-terminal (IPR023123)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014134 AAF51174   AC010009   AC092187   AF073994 AAC27620   AI136006   AI406263   AI514354   AI531067   AW943499   AY069633 AAL39778   BF485702   BF485718   BF485719   BF485743   BF485744   BF487180   BF495310   BI354266   BI637398   BI640028   BP547240   BX011263   CO152833   CO181861   CO194465   CO278028   CO309403   CO325068   CO327885   CO330384   CO331621   CO332236   EC074882   EC077676   EC214847   EC228082   EC233123   EC239723   EC247819   EC256579   GH941587   GH947093   GH948720   GH952105   GH952804   GH954171   GH954206   GH955121   GH956228   GH959278   GH960394   GH960462   GH961923   GH961939   GH962310   GH963072   GH963246   GH963656   GH966012   GH966304   GH966382   GH966429   GH967555   GH970398   GH972208   GH974389   GH975259   GH976485   GH976619   GH979166   GH980462   GH980751   GH983461   GH983680   M61765 AAA28597
UniProtKB/Swiss-Prot	P23257
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | faint & ubiquitous   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   organism | faint & ubiquitous   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
cytoplasm inferred from direct assay (Dobbelaere et al., 2008) gamma-tubulin complex inferred from physical interaction with Grip128 (Wiese, 2008) inferred from physical interaction with Grip163 (Wiese, 2008) gamma-tubulin large complex inferred from direct assay (Wiese, 2008) gamma-tubulin ring complex inferred from direct assay (Wiese, 2008) gamma-tubulin small complex inferred from physical interaction with Grip84 (Gunawardane et al., 2000) inferred from physical interaction with l(1)dd4 (Gunawardane et al., 2000) microtubule inferred from direct assay (Dobbelaere et al., 2008) pericentriolar material inferred from direct assay (Dobbelaere et al., 2008) spindle inferred from direct assay (Dobbelaere et al., 2008)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, larval trachea, adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    361.05 Larval Midgut    29.8 Larval Hindgut    80.9 Larval Malpighian Tubules    54.4 Larval Fat Body    59.2 Larval Salivary Gland    21.7 Larval Trachea    123.875 Larval Carcass    80.05 Adult Head    76.3 Adult Eye    78.3 Adult Brain    190.5 Adult Thoracic-Abdominal Ganglion    207.4 Adult Crop    90.5 Adult Midgut    36.5 Adult Hindgut    70.6 Adult Malpighian Tubules    32.3 Adult Fat Body    30.9 Adult Salivary Gland    21.3 Adult Heart    55.65 Adult VirginFemale Spermatheca    15.2 Adult InseminatedFemale Spermatheca    20.5 Adult Ovary    201.6 Adult Testis    83.6 Adult Male Accessory Gland    17.2 Adult Carcass    50.7 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    361.05 Larval Midgut    29.8 Larval Hindgut    80.9 Larval Malpighian Tubules    54.4 Larval Fat Body    59.2 Larval Salivary Gland    21.7 Larval Trachea    123.875 Larval Carcass    80.05 Adult Head    76.3 Adult Eye    78.3 Adult Brain    190.5 Adult Thoracic-Abdominal Ganglion    207.4 Adult Crop    90.5 Adult Midgut    36.5 Adult Hindgut    70.6 Adult Malpighian Tubules    32.3 Adult Fat Body    30.9 Adult Salivary Gland    21.3 Adult Heart    55.65 Adult VirginFemale Spermatheca    15.2 Adult InseminatedFemale Spermatheca    20.5 Adult Ovary    201.6 Adult Testis    83.6 Adult Male Accessory Gland    17.2 Adult Carcass    50.7 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    361.05 Larval Midgut    29.8 Larval Hindgut    80.9 Larval Malpighian Tubules    54.4 Larval Fat Body    59.2 Larval Salivary Gland    21.7 Larval Trachea    123.875 Larval Carcass    80.05 Adult Head    76.3 Adult Eye    78.3 Adult Brain    190.5 Adult Thoracic-Abdominal Ganglion    207.4 Adult Crop    90.5 Adult Midgut    36.5 Adult Hindgut    70.6 Adult Malpighian Tubules    32.3 Adult Fat Body    30.9 Adult Salivary Gland    21.3 Adult Heart    55.65 Adult VirginFemale Spermatheca    15.2 Adult InseminatedFemale Spermatheca    20.5 Adult Ovary    201.6 Adult Testis    83.6 Adult Male Accessory Gland    17.2 Adult Carcass    50.7 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    361.05 Larval Midgut    29.8 Larval Hindgut    80.9 Larval Malpighian Tubules    54.4 Larval Fat Body    59.2 Larval Salivary Gland    21.7 Larval Trachea    123.875 Larval Carcass    80.05 Adult Head    76.3 Adult Eye    78.3 Adult Brain    190.5 Adult Thoracic-Abdominal Ganglion    207.4 Adult Crop    90.5 Adult Midgut    36.5 Adult Hindgut    70.6 Adult Malpighian Tubules    32.3 Adult Fat Body    30.9 Adult Salivary Gland    21.3 Adult Heart    55.65 Adult VirginFemale Spermatheca    15.2 Adult InseminatedFemale Spermatheca    20.5 Adult Ovary    201.6 Adult Testis    83.6 Adult Male Accessory Gland    17.2 Adult Carcass    50.7 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    361.05 Larval Midgut    29.8 Larval Hindgut    80.9 Larval Malpighian Tubules    54.4 Larval Fat Body    59.2 Larval Salivary Gland    21.7 Larval Trachea    123.875 Larval Carcass    80.05 Adult Head    76.3 Adult Eye    78.3 Adult Brain    190.5 Adult Thoracic-Abdominal Ganglion    207.4 Adult Crop    90.5 Adult Midgut    36.5 Adult Hindgut    70.6 Adult Malpighian Tubules    32.3 Adult Fat Body    30.9 Adult Salivary Gland    21.3 Adult Heart    55.65 Adult VirginFemale Spermatheca    15.2 Adult InseminatedFemale Spermatheca    20.5 Adult Ovary    201.6 Adult Testis    83.6 Adult Male Accessory Gland    17.2 Adult Carcass    50.7 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    361.05 Larval Midgut    29.8 Larval Hindgut    80.9 Larval Malpighian Tubules    54.4 Larval Fat Body    59.2 Larval Salivary Gland    21.7 Larval Trachea    123.875 Larval Carcass    80.05 Adult Head    76.3 Adult Eye    78.3 Adult Brain    190.5 Adult Thoracic-Abdominal Ganglion    207.4 Adult Crop    90.5 Adult Midgut    36.5 Adult Hindgut    70.6 Adult Malpighian Tubules    32.3 Adult Fat Body    30.9 Adult Salivary Gland    21.3 Adult Heart    55.65 Adult VirginFemale Spermatheca    15.2 Adult InseminatedFemale Spermatheca    20.5 Adult Ovary    201.6 Adult Testis    83.6 Adult Male Accessory Gland    17.2 Adult Carcass    50.7 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    361.05 Larval Midgut    29.8 Larval Hindgut    80.9 Larval Malpighian Tubules    54.4 Larval Fat Body    59.2 Larval Salivary Gland    21.7 Larval Trachea    123.875 Larval Carcass    80.05 Adult Head    76.3 Adult Eye    78.3 Adult Brain    190.5 Adult Thoracic-Abdominal Ganglion    207.4 Adult Crop    90.5 Adult Midgut    36.5 Adult Hindgut    70.6 Adult Malpighian Tubules    32.3 Adult Fat Body    30.9 Adult Salivary Gland    21.3 Adult Heart    55.65 Adult VirginFemale Spermatheca    15.2 Adult InseminatedFemale Spermatheca    20.5 Adult Ovary    201.6 Adult Testis    83.6 Adult Male Accessory Gland    17.2 Adult Carcass    50.7 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    361.05 Larval Midgut    29.8 Larval Hindgut    80.9 Larval Malpighian Tubules    54.4 Larval Fat Body    59.2 Larval Salivary Gland    21.7 Larval Trachea    123.875 Larval Carcass    80.05 Adult Head    76.3 Adult Eye    78.3 Adult Brain    190.5 Adult Thoracic-Abdominal Ganglion    207.4 Adult Crop    90.5 Adult Midgut    36.5 Adult Hindgut    70.6 Adult Malpighian Tubules    32.3 Adult Fat Body    30.9 Adult Salivary Gland    21.3 Adult Heart    55.65 Adult VirginFemale Spermatheca    15.2 Adult InseminatedFemale Spermatheca    20.5 Adult Ovary    201.6 Adult Testis    83.6 Adult Male Accessory Gland    17.2 Adult Carcass    50.7 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    76.3   NA  Eye    78.3   NA  Brain    190.5   361.05  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    207.4   NA  Crop    90.5   29.8  Midgut    36.5   80.9  Hindgut    70.6   54.4  Malpighian Tubules    32.3   59.2  Fat Body    30.9   21.7  Salivary Gland    no informative data   NA  Heart    55.65   123.875  Trachea    NA   NA  VirginFemale Spermatheca    15.2   NA  InseminatedFemale Spermatheca    no informative data   NA  Ovary    201.6   NA  Testis    83.6   NA  Male Accessory Gland    17.2   80.05  Carcass    50.7 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    21 central nervous system, larvae L3    18 central nervous system, pupae P8    10 head, virgin 1-day female    5 head, virgin 4-day female    4 head, virgin 20-day female    5 head, mated 1-day female    5 head, mated 4-day female    4 head, mated 20-day female    4 head, mated 1-day male    6 head, mated 4-day male    7 head, mated 20-day male    10 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    7 carcass, larvae L3 wandering    15 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    2 ovary, virgin 4-day female    27 ovary, mated 4-day female    19 testis, mated 4-day male    8 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    21 central nervous system, larvae L3    18 central nervous system, pupae P8    10 head, virgin 1-day female    5 head, virgin 4-day female    4 head, virgin 20-day female    5 head, mated 1-day female    5 head, mated 4-day female    4 head, mated 20-day female    4 head, mated 1-day male    6 head, mated 4-day male    7 head, mated 20-day male    10 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    7 carcass, larvae L3 wandering    15 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    2 ovary, virgin 4-day female  (27) ovary, mated 4-day female    19 testis, mated 4-day male    8 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    21 central nervous system, larvae L3    18 central nervous system, pupae P8    10 head, virgin 1-day female    5 head, virgin 4-day female    4 head, virgin 20-day female    5 head, mated 1-day female    5 head, mated 4-day female    4 head, mated 20-day female    4 head, mated 1-day male    6 head, mated 4-day male    7 head, mated 20-day male    10 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    7 carcass, larvae L3 wandering    15 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    2 ovary, virgin 4-day female    27 ovary, mated 4-day female    19 testis, mated 4-day male    8 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    21 central nervous system, larvae L3    18 central nervous system, pupae P8    10 head, virgin 1-day female    5 head, virgin 4-day female    4 head, virgin 20-day female    5 head, mated 1-day female    5 head, mated 4-day female    4 head, mated 20-day female    4 head, mated 1-day male    6 head, mated 4-day male    7 head, mated 20-day male    10 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    7 carcass, larvae L3 wandering    15 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    2 ovary, virgin 4-day female    27 ovary, mated 4-day female    19 testis, mated 4-day male    8 accessory gland, mated 4-day male    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    21 central nervous system, larvae L3    18 central nervous system, pupae P8    10 head, virgin 1-day female    5 head, virgin 4-day female    4 head, virgin 20-day female    5 head, mated 1-day female    5 head, mated 4-day female    4 head, mated 20-day female    4 head, mated 1-day male    6 head, mated 4-day male    7 head, mated 20-day male    10 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    7 carcass, larvae L3 wandering    15 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    2 ovary, virgin 4-day female    27 ovary, mated 4-day female    19 testis, mated 4-day male    8 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    21 central nervous system, larvae L3    18 central nervous system, pupae P8    10 head, virgin 1-day female    5 head, virgin 4-day female    4 head, virgin 20-day female    5 head, mated 1-day female    5 head, mated 4-day female    4 head, mated 20-day female    4 head, mated 1-day male    6 head, mated 4-day male    7 head, mated 20-day male    10 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    7 carcass, larvae L3 wandering    15 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    2 ovary, virgin 4-day female  27 ovary, mated 4-day female    19 testis, mated 4-day male    8 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    21 central nervous system, larvae L3    18 central nervous system, pupae P8    10 head, virgin 1-day female    5 head, virgin 4-day female    4 head, virgin 20-day female    5 head, mated 1-day female    5 head, mated 4-day female    4 head, mated 20-day female    4 head, mated 1-day male    6 head, mated 4-day male    7 head, mated 20-day male    10 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    7 carcass, larvae L3 wandering    15 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    2 ovary, virgin 4-day female    27 ovary, mated 4-day female    19 testis, mated 4-day male    8 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    21 central nervous system, larvae L3    18 central nervous system, pupae P8    10 head, virgin 1-day female    5 head, virgin 4-day female    4 head, virgin 20-day female    5 head, mated 1-day female    5 head, mated 4-day female    4 head, mated 20-day female    4 head, mated 1-day male    6 head, mated 4-day male    7 head, mated 20-day male    10 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    5 fat body, pupae P8    7 carcass, larvae L3 wandering    15 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    2 ovary, virgin 4-day female    27 ovary, mated 4-day female    19 testis, mated 4-day male    8 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    59 embryo 02-04hr    79 embryo 04-06hr    66 embryo 06-08hr    43 embryo 08-10hr    33 embryo 10-12hr    21 embryo 12-14hr    13 embryo 14-16hr    8 embryo 16-18hr    5 embryo 18-20hr    5 embryo 20-22hr    4 embryo 22-24hr    5 larva L1    6 larva L2    7 larva L3 12hr old    7 larva L3 puffstage 1-2    13 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    24 white prepupae new    22 white prepupae 12hr    26 white prepupae 24hr    30 pupae 2d postWPP    18 pupae 3d postWPP    14 pupae 4d postWPP    7 adult male 01day    10 adult male 05day    12 adult male 30day    13 adult female 01day    11 adult female 05day    15 adult female 30day    16 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (59) embryo 02-04hr  (79) embryo 04-06hr  (66) embryo 06-08hr  (43) embryo 08-10hr  (33) embryo 10-12hr    21 embryo 12-14hr    13 embryo 14-16hr    8 embryo 16-18hr    5 embryo 18-20hr    5 embryo 20-22hr    4 embryo 22-24hr    5 larva L1    6 larva L2    7 larva L3 12hr old    7 larva L3 puffstage 1-2    13 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    24 white prepupae new    22 white prepupae 12hr  26 white prepupae 24hr  (30) pupae 2d postWPP    18 pupae 3d postWPP    14 pupae 4d postWPP    7 adult male 01day    10 adult male 05day    12 adult male 30day    13 adult female 01day    11 adult female 05day    15 adult female 30day    16 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    59 embryo 02-04hr    79 embryo 04-06hr    66 embryo 06-08hr    43 embryo 08-10hr    33 embryo 10-12hr    21 embryo 12-14hr    13 embryo 14-16hr    8 embryo 16-18hr    5 embryo 18-20hr    5 embryo 20-22hr    4 embryo 22-24hr    5 larva L1    6 larva L2    7 larva L3 12hr old    7 larva L3 puffstage 1-2    13 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    24 white prepupae new    22 white prepupae 12hr    26 white prepupae 24hr    30 pupae 2d postWPP    18 pupae 3d postWPP    14 pupae 4d postWPP    7 adult male 01day    10 adult male 05day    12 adult male 30day    13 adult female 01day    11 adult female 05day    15 adult female 30day    16 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    59 embryo 02-04hr    79 embryo 04-06hr    66 embryo 06-08hr    43 embryo 08-10hr    33 embryo 10-12hr    21 embryo 12-14hr    13 embryo 14-16hr    8 embryo 16-18hr    5 embryo 18-20hr    5 embryo 20-22hr    4 embryo 22-24hr    5 larva L1    6 larva L2    7 larva L3 12hr old    7 larva L3 puffstage 1-2    13 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    24 white prepupae new    22 white prepupae 12hr    26 white prepupae 24hr    30 pupae 2d postWPP    18 pupae 3d postWPP    14 pupae 4d postWPP    7 adult male 01day    10 adult male 05day    12 adult male 30day    13 adult female 01day    11 adult female 05day    15 adult female 30day    16 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    59 embryo 02-04hr    79 embryo 04-06hr    66 embryo 06-08hr    43 embryo 08-10hr    33 embryo 10-12hr    21 embryo 12-14hr    13 embryo 14-16hr    8 embryo 16-18hr    5 embryo 18-20hr    5 embryo 20-22hr    4 embryo 22-24hr    5 larva L1    6 larva L2    7 larva L3 12hr old    7 larva L3 puffstage 1-2    13 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    24 white prepupae new    22 white prepupae 12hr    26 white prepupae 24hr    30 pupae 2d postWPP    18 pupae 3d postWPP    14 pupae 4d postWPP    7 adult male 01day    10 adult male 05day    12 adult male 30day    13 adult female 01day    11 adult female 05day    15 adult female 30day    16 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (59) embryo 02-04hr  (79) embryo 04-06hr  (66) embryo 06-08hr  (43) embryo 08-10hr  (33) embryo 10-12hr    21 embryo 12-14hr    13 embryo 14-16hr    8 embryo 16-18hr    5 embryo 18-20hr    5 embryo 20-22hr    4 embryo 22-24hr    5 larva L1    6 larva L2    7 larva L3 12hr old    7 larva L3 puffstage 1-2    13 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    24 white prepupae new    22 white prepupae 12hr  26 white prepupae 24hr  30 pupae 2d postWPP    18 pupae 3d postWPP    14 pupae 4d postWPP    7 adult male 01day    10 adult male 05day    12 adult male 30day    13 adult female 01day    11 adult female 05day    15 adult female 30day    16 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    59 embryo 02-04hr    79 embryo 04-06hr    66 embryo 06-08hr    43 embryo 08-10hr    33 embryo 10-12hr    21 embryo 12-14hr    13 embryo 14-16hr    8 embryo 16-18hr    5 embryo 18-20hr    5 embryo 20-22hr    4 embryo 22-24hr    5 larva L1    6 larva L2    7 larva L3 12hr old    7 larva L3 puffstage 1-2    13 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    24 white prepupae new    22 white prepupae 12hr    26 white prepupae 24hr    30 pupae 2d postWPP    18 pupae 3d postWPP    14 pupae 4d postWPP    7 adult male 01day    10 adult male 05day    12 adult male 30day    13 adult female 01day    11 adult female 05day    15 adult female 30day    16 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    59 embryo 02-04hr    79 embryo 04-06hr    66 embryo 06-08hr    43 embryo 08-10hr    33 embryo 10-12hr    21 embryo 12-14hr    13 embryo 14-16hr    8 embryo 16-18hr    5 embryo 18-20hr    5 embryo 20-22hr    4 embryo 22-24hr    5 larva L1    6 larva L2    7 larva L3 12hr old    7 larva L3 puffstage 1-2    13 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    24 white prepupae new    22 white prepupae 12hr    26 white prepupae 24hr    30 pupae 2d postWPP    18 pupae 3d postWPP    14 pupae 4d postWPP    7 adult male 01day    10 adult male 05day    12 adult male 30day    13 adult female 01day    11 adult female 05day    15 adult female 30day    16 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    43 Schneider line 2 Sg4    43 embryonic 1182-4H    23 embryonic GM2    22 embryonic Kc167    19 embryonic S1    40 embryonic S3    28 leg disc CME L1    32 wing disc CME-W2    35 wing disc ML-DmD8    30 wing disc ML-DmD9    37 wing disc ML-DmD16-c3    21 wing disc ML-DmD21    38 wing disc ML-DmD32    26 haltere disc ML-DmD17-c3    40 eye-antennal disc ML-DmD11    30 antennal disc ML-DmD20-c5    34 mixed discs ML-DmD4-c1    24 CNS ML-DmBG1-c1    21 CNS ML-DmBG2-c2    25 tumorous blood cells mbn2    15 ovary fGS/OSS    16 ovary OSC    16 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (43) Schneider line 2 Sg4  (43) embryonic 1182-4H    23 embryonic GM2    22 embryonic Kc167    19 embryonic S1  (40) embryonic S3  (28) leg disc CME L1  (32) wing disc CME-W2  (35) wing disc ML-DmD8  (30) wing disc ML-DmD9  (37) wing disc ML-DmD16-c3    21 wing disc ML-DmD21  (38) wing disc ML-DmD32  26 haltere disc ML-DmD17-c3  (40) eye-antennal disc ML-DmD11  (30) antennal disc ML-DmD20-c5  (34) mixed discs ML-DmD4-c1    24 CNS ML-DmBG1-c1    21 CNS ML-DmBG2-c2    25 tumorous blood cells mbn2    15 ovary fGS/OSS    16 ovary OSC    16 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    43 Schneider line 2 Sg4    43 embryonic 1182-4H    23 embryonic GM2    22 embryonic Kc167    19 embryonic S1    40 embryonic S3    28 leg disc CME L1    32 wing disc CME-W2    35 wing disc ML-DmD8    30 wing disc ML-DmD9    37 wing disc ML-DmD16-c3    21 wing disc ML-DmD21    38 wing disc ML-DmD32    26 haltere disc ML-DmD17-c3    40 eye-antennal disc ML-DmD11    30 antennal disc ML-DmD20-c5    34 mixed discs ML-DmD4-c1    24 CNS ML-DmBG1-c1    21 CNS ML-DmBG2-c2    25 tumorous blood cells mbn2    15 ovary fGS/OSS    16 ovary OSC    16 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    43 Schneider line 2 Sg4    43 embryonic 1182-4H    23 embryonic GM2    22 embryonic Kc167    19 embryonic S1    40 embryonic S3    28 leg disc CME L1    32 wing disc CME-W2    35 wing disc ML-DmD8    30 wing disc ML-DmD9    37 wing disc ML-DmD16-c3    21 wing disc ML-DmD21    38 wing disc ML-DmD32    26 haltere disc ML-DmD17-c3    40 eye-antennal disc ML-DmD11    30 antennal disc ML-DmD20-c5    34 mixed discs ML-DmD4-c1    24 CNS ML-DmBG1-c1    21 CNS ML-DmBG2-c2    25 tumorous blood cells mbn2    15 ovary fGS/OSS    16 ovary OSC    16 ovary OSS    8 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    43 Schneider line 2 Sg4    43 embryonic 1182-4H    23 embryonic GM2    22 embryonic Kc167    19 embryonic S1    40 embryonic S3    28 leg disc CME L1    32 wing disc CME-W2    35 wing disc ML-DmD8    30 wing disc ML-DmD9    37 wing disc ML-DmD16-c3    21 wing disc ML-DmD21    38 wing disc ML-DmD32    26 haltere disc ML-DmD17-c3    40 eye-antennal disc ML-DmD11    30 antennal disc ML-DmD20-c5    34 mixed discs ML-DmD4-c1    24 CNS ML-DmBG1-c1    21 CNS ML-DmBG2-c2    25 tumorous blood cells mbn2    15 ovary fGS/OSS    16 ovary OSC    16 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (43) Schneider line 2 Sg4  (43) embryonic 1182-4H    23 embryonic GM2    22 embryonic Kc167    19 embryonic S1  (40) embryonic S3  28 leg disc CME L1  32 wing disc CME-W2  (35) wing disc ML-DmD8  30 wing disc ML-DmD9  (37) wing disc ML-DmD16-c3    21 wing disc ML-DmD21  (38) wing disc ML-DmD32  26 haltere disc ML-DmD17-c3  (40) eye-antennal disc ML-DmD11  30 antennal disc ML-DmD20-c5  (34) mixed discs ML-DmD4-c1    24 CNS ML-DmBG1-c1    21 CNS ML-DmBG2-c2    25 tumorous blood cells mbn2    15 ovary fGS/OSS    16 ovary OSC    16 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    43 Schneider line 2 Sg4    43 embryonic 1182-4H    23 embryonic GM2    22 embryonic Kc167    19 embryonic S1    40 embryonic S3    28 leg disc CME L1    32 wing disc CME-W2    35 wing disc ML-DmD8    30 wing disc ML-DmD9    37 wing disc ML-DmD16-c3    21 wing disc ML-DmD21    38 wing disc ML-DmD32    26 haltere disc ML-DmD17-c3    40 eye-antennal disc ML-DmD11    30 antennal disc ML-DmD20-c5    34 mixed discs ML-DmD4-c1    24 CNS ML-DmBG1-c1    21 CNS ML-DmBG2-c2    25 tumorous blood cells mbn2    15 ovary fGS/OSS    16 ovary OSC    16 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    43 Schneider line 2 Sg4    43 embryonic 1182-4H    23 embryonic GM2    22 embryonic Kc167    19 embryonic S1    40 embryonic S3    28 leg disc CME L1    32 wing disc CME-W2    35 wing disc ML-DmD8    30 wing disc ML-DmD9    37 wing disc ML-DmD16-c3    21 wing disc ML-DmD21    38 wing disc ML-DmD32    26 haltere disc ML-DmD17-c3    40 eye-antennal disc ML-DmD11    30 antennal disc ML-DmD20-c5    34 mixed discs ML-DmD4-c1    24 CNS ML-DmBG1-c1    21 CNS ML-DmBG2-c2    25 tumorous blood cells mbn2    15 ovary fGS/OSS    16 ovary OSC    16 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    9 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    11 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    8 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    20 Ethanol 5% 3 hrs, larvae L3    6 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    15 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    9 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    11 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    8 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    20 Ethanol 5% 3 hrs, larvae L3    6 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    15 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    9 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    11 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    8 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    20 Ethanol 5% 3 hrs, larvae L3    6 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    15 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    9 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    11 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    8 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    20 Ethanol 5% 3 hrs, larvae L3    6 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    15 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    9 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    11 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    8 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    20 Ethanol 5% 3 hrs, larvae L3    6 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    15 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    9 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    11 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    8 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    20 Ethanol 5% 3 hrs, larvae L3    6 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    15 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    9 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    11 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    8 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    20 Ethanol 5% 3 hrs, larvae L3    6 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    15 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    9 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    11 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    19 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    8 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    20 Ethanol 5% 3 hrs, larvae L3    6 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    15 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_06 mE1_20_mRNA_expression_cluster_04
External Data & Images
Linkouts	BDGP expression data • LD40196 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0260639
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal γTub23CΔ lethal (with Df(2L)3G) γTub23CA6-2 γTub23CA15-2 γTub23Cbmps1 γTub23CPl-2 lethal (with Df(2L)JS17) γTub23CA6-2 γTub23CA15-2 γTub23Cbmps1 γTub23CPl-2 lethal (with γTub23CA6-2) γTub23CA15-2 γTub23Cbmps1 γTub23CPl-2 lethal (with γTub23CA14-9) γTub23Cbmps1 γTub23CPl-2 lethal (with γTub23CA15-2) γTub23CA6-2 γTub23Cbmps1 γTub23CPl-2 lethal (with γTub23Cbmps1) γTub23CA6-2 γTub23CA14-9 γTub23CA15-2 γTub23CPl-2 lethal (with γTub23CPl-2) γTub23CA6-2 γTub23CA14-9 γTub23CA15-2 γTub23Cbmps1 lethal | larval stage | recessive γTub23CPI lethal | recessive γTub23CA6-2 γTub23CA14-9 γTub23CA15-2 viable γTub23C+t9 viable, with Scer\GAL4pnr-MD237 γTub23CGD8749 γTub23CGD16397 Other Phenotypes Allele mitotic cell cycle defective γTub23CΔ mitotic cell cycle defective | recessive γTub23CPI visible (with Df(2L)3G) γTub23CA14-9 visible | dominant γTub23Cbmps1 γTub23Cbmps2 γTub23Cbmps4 γTub23CPl-2 γTub23Cunspecified visible | dominant | heat sensitive γTub23Cbmps1 γTub23Cbmps4 wild-type γTub23C+t9 Phenotype manifest in Allele embryonic/larval brain γTub23CPI eye γTub23CPl-2 γTub23Cbmps1 γTub23Cbmps2 γTub23Cbmps4 imaginal disc γTub23CPI sensillum campaniformium of dorsal radius | P-stage γTub23Cbmps1 spermatid γTub23CPI spermatocyte & aster γTub23CPI spermatocyte & spindle γTub23CPI spindle γTub23CdsRNA.cMa spindle microtubule γTub23CdsRNA.cMa wing γTub23CPl-2 γTub23Cbmps1 γTub23Cbmps4 wing (with Df(2L)3G) γTub23CA14-9 wing | heat sensitive γTub23Cbmps1 γTub23Cbmps4 wing blade γTub23CPl-2 γTub23Cunspecified wing margin γTub23CPl-2 wing vein γTub23CPl-2 wing vein L2 γTub23CPl-2 wing vein L3 γTub23CPl-2
Classical Alleles ( 11 )
For All Classical Alleles Show
Allele of γTub23C Class Mutagen Stocks Known lesion γTub23CA6-2 ethyl methanesulfonate 2 Yes γTub23CA14-9 hypomorphic allele - genetic evidence ethyl methanesulfonate 1 Yes γTub23CA15-2 loss of function allele ethyl methanesulfonate 1 Yes γTub23Cbmps1 ethyl methanesulfonate 1 Yes γTub23Cbmps2 ethyl methanesulfonate 0 Yes γTub23Cbmps3 ethyl methanesulfonate 0 Yes γTub23Cbmps4 ethyl methanesulfonate 0 Yes γTub23Cbmps5 ethyl methanesulfonate 0 Yes γTub23CPI loss of function allele P-element activity 0 Yes γTub23CPl-2 antimorphic allele - genetic evidence 0 Yes γTub23Cunspecified 0 --
Alleles Carried on Transgenic Constructs ( 12 )
For All Alleles Carried on Transgenic Constructs Show
Allele of γTub23C Class Mutagen Stocks Known lesion γTub23CGD16397 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes γTub23CGD8749 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes γTub23CGL01171 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes γTub23CJF01722 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes γTub23CKK107500 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes γTub23C+t9 in vitro construct - genomic fragment 0 Yes γTub23C+tLa in vitro construct - genomic fragment 0 Yes γTub23CAct5C.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes γTub23CdsRNA.cMa in vitro construct - RNAi 0 -- γTub23CNIG.3157R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes γTub23CScer\UAS.T:Avic\GFP 0 Yes γTub23CΔ in vitro construct - genomic fragment 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)3G (Vázquez et al., 2008) Df(2L)AS2 (Ferro, 2002.12) Df(2L)JS17 (Eeken, 2000.2.29, Ferro, 2002.12, Vázquez et al., 2008) Df(2L)JS31 (Vázquez et al., 2008)
Not disrupted in	Df(2L)16 (Ferro, 2002.12) Df(2L)17 (Ferro, 2002.12) Df(2L)18 (Ferro, 2002.12) Df(2L)19 (Ferro, 2002.12) Df(2L)20 (Ferro, 2002.12) Df(2L)AS8 (Ferro, 2002.12) Df(2L)C28 (Eeken, 2000.2.29, Ferro, 2002.12, Vázquez et al., 2008) Df(2L)C144 (Vázquez et al., 2008) Df(2L)JS7 (Eeken, 2000.2.29, Vázquez et al., 2008) Df(2L)JS25 (Ferro, 2002.12) Df(2L)JS27 (Ferro, 2002.12) Df(2L)S2590 (Vázquez et al., 2008) Ts(2Rt;3Lt)DTD62+Ts(2Lt;3Rt)HVD7 (Vázquez et al., 2008)
Duplicated in	Dp(2;1)JS13 (Vázquez et al., 2008)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data characterization construct P{w-γ-23C} NA P{w-γ-23CΔ} NA P{γTub23C+tLa} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{hsneo}γTub23CPI NA
Gene Ontology: Function, Process & Cellular Component ( 26 unique terms )
Terms Based on Experimental Evidence ( 19 terms )
Molecular Function
CV term Evidence References
GTP binding inferred from direct assay (Gunawardane et al., 2003) guanyl nucleotide binding inferred from direct assay (Gunawardane et al., 2000) contributes_to microtubule minus-end binding inferred from direct assay (Gunawardane et al., 2000)
Biological Process
CV term Evidence References
centriole-centriole cohesion inferred from direct assay (Raynaud-Messina et al., 2004) centrosome duplication inferred from mutant phenotype (Müller et al., 2010) centrosome organization inferred from mutant phenotype (Goshima et al., 2007, Müller et al., 2010, Dobbelaere et al., 2008) microtubule nucleation inferred from direct assay (Gunawardane et al., 2000) inferred from mutant phenotype (Mahoney et al., 2006, M Mahoney, 2006) mitosis inferred from mutant phenotype (Ducat et al., 2008) mitotic sister chromatid separation inferred from direct assay (Raynaud-Messina et al., 2004) mitotic spindle organization inferred from mutant phenotype (Goshima et al., 2007) regulation of cell cycle inferred from mutant phenotype (Muller et al., 2006)
Cellular Component
CV term Evidence References
cytoplasm inferred from direct assay (Dobbelaere et al., 2008) gamma-tubulin complex inferred from physical interaction with Grip128 (Wiese, 2008) inferred from physical interaction with Grip163 (Wiese, 2008) gamma-tubulin large complex inferred from direct assay (Wiese, 2008) gamma-tubulin ring complex inferred from direct assay (Wiese, 2008) gamma-tubulin small complex inferred from physical interaction with Grip84 (Gunawardane et al., 2000) inferred from physical interaction with l(1)dd4 (Gunawardane et al., 2000) microtubule inferred from direct assay (Dobbelaere et al., 2008) pericentriolar material inferred from direct assay (Dobbelaere et al., 2008) spindle inferred from direct assay (Dobbelaere et al., 2008)
Terms Based on Predictions or Assertions ( 9 terms )
Molecular Function
CV term Evidence References
GTPase activity inferred from electronic annotation with InterPro:IPR008280, InterPro:IPR018316 (FlyBase Curators et al., 2004-) structural constituent of cytoskeleton inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000, Goldstein and Gunawardena, 2000) inferred from sequence or structural similarity with UniProtKB:P23330 (FlyBase, 1992-)
Biological Process
CV term Evidence References
GTP catabolic process inferred from electronic annotation with InterPro:IPR008280, InterPro:IPR018316 (FlyBase Curators et al., 2004-) microtubule-based process inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000) non-traceable author statement (Swiss-Prot Project Members, 1991.11.1) protein polymerization inferred from electronic annotation with InterPro:IPR003008, InterPro:IPR008280, InterPro:IPR018316 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
gamma-tubulin small complex inferred from sequence or structural similarity with UniProtKB:P23330 (FlyBase, 1992-) microtubule inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000, Goldstein and Gunawardena, 2000) inferred from sequence or structural similarity with UniProtKB:P23330 (FlyBase, 1992-) microtubule organizing center inferred from sequence or structural similarity with UniProtKB:P23330 (FlyBase, 1992-) non-traceable author statement (Swiss-Prot Project Members, 1991.11.1) tubulin complex non-traceable author statement (Swiss-Prot Project Members, 1991.11.1)
Sequence Ontology: Class of Gene
	gene   nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
γTub23C - CG15220 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) γTub23C - CG5525 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) γTub23C - CG7033 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) γTub23C - CG7766 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) γTub23C - CG8258 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) γTub23C - Cct5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) γTub23C - Cctγ anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) γTub23C - Fatp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) γTub23C - Grip75 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) γTub23C - Grip84 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) γTub23C - Rpb8 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) γTub23C - Rpp30 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) γTub23C - Tcp-1ζ anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) γTub23C - Tcp-1η anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) γTub23C - mael anti bait coimmunoprecipitation, western blot (Sato et al., 2011)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions γTub23C allele Gene References γTub23CPI γTub37C (Tavosanis and Gonzalez, 2003) unspecified brm (Vazquez et al., 2006) N (Mahoney et al., 2006, Vazquez et al., 2006) Psn (Mahoney et al., 2006)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 59721 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0260639 DroID - A comprehensive database of gene and protein interactions. • FBgn0260639 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 33501
Orthologs
OrthoDB Orthologs (41) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6575SB Dipteran inclusive ortholog search EOG6JT0DS Insect inclusive ortholog search EOG6KPS66 Arthropod inclusive ortholog search EOG6ZPC9H Metazoa inclusive ortholog search EOG6M0STZ
Orthologs in Drosophila Species (EOG6575SB)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\γTub23C     Drosophila simulans   Dsim\GD23196 Y   Drosophila sechellia   Dsec\GM18381 Y   Drosophila erecta   Dere\GG24903 Y   Drosophila yakuba   Dyak\GE18195 Y   Drosophila ananassae   Dana\GF14993 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA16328 Y   Drosophila persimilis   Dper\GL26300 Y   Drosophila willistoni   Dwil\GK24850 Y   Drosophila virilis   Dvir\GJ17947 Y   Drosophila mojavensis   Dmoj\GI17602 Y   Drosophila grimshawi   Dgri\GH11085
Orthologs in non-Drosophila Dipterans (EOG6JT0DS)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL012843 Y Aedes aegypti Yellow fever mosquito  Aaeg\AAEL013064 Y Anopheles gambiae Malaria mosquito  Agam\AGAP007834 Y Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ005487 Y
Orthologs in non-Dipteran Insects (EOG6KPS66)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\TUBG1 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG013088 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH18693 Y Atta cephalotes Leafcutter ant  Acep\ACEP14750 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO19306 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL21946 Y Linepithema humile Argentine ant  Lhum\LH19286 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB24138 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI004513 Y Bombyx mori Silkmoth  Bmor\BGIBMGA013500 Y Pediculus humanus Human body louse  Phum\PHUM087820 Y Tribolium castaneum Red flour beetle  Tcas\GLEAN_14883 Y
Orthologs in non-Insect Arthropods (EOG6ZPC9H)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\Clec3b Y Ixodes scapularis Deer tick  Isca\ISCW023762 Y
Orthologs in non-Arthropod Metazoa (EOG6M0STZ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\tbg-1 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_020943gn Y Danio rerio Zebrafish  Drer\tubgl Y Xenopus tropicalis Western clawed frog  Xtro\tubg1 Y Gallus gallus Domestic chicken  Ggal\TUBG1 Y Mus musculus House mouse  Mmus\Tubg2 Y Mus musculus House mouse  Mmus\Tubg1 Y Rattus norvegicus Norway rat  Rnor\LOC688197 Y Rattus norvegicus Norway rat  Rnor\Tubg1 Y Homo sapiens Human  Hsap\TUBG2 Y Homo sapiens Human  Hsap\TUBG1 Y
Human Orthologs (2)
Gene OMIM HGNC Hsap\TUBG2     Hsap\TUBG1
AAA Orthologs (10) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD23196 Drosophila sechellia Dsec\GM18381 Drosophila erecta Dere\GG24903 Drosophila yakuba Dyak\GE18195 Drosophila ananassae Dana\GF14993 Drosophila pseudoobscura pseudoobscura Dpse\GA16328 Drosophila persimilis Dper\GL26300 Drosophila willistoni Dwil\GK24850 Drosophila virilis Dvir\GJ17947 Drosophila mojavensis Dmoj\GI17602
Stocks & Reagents
Stocks Listed in FlyBase ( 10 )
Bloomington	31204 y1 v1; P{TRiP.JF01722}attP2 42799 y1 v1; P{TRiP.GL01171}attP2 8399 l(1)dd4su(Pl); γTub23Cbmps1/CyO 5728 γTub23CA6-2 cn1 bw1/CyO 7041 γTub23CA14-9 cn1 bw1/CyO 7042 γTub23CA15-2 cn1 bw1/CyO
Kyoto	108391 γTub23CA6-2 cn1 bw1/CyO
VDRC	v107572 P{KK107500}VIE-260B v19130 w1118; P{GD8749}v19130 v47163 w1118 P{GD16397}v47163
Genomic Clones ( 1 )
	BACR23N06 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 60 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD40196
Other clones	DPiM_LD40196
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT04292 AT19648 AT19670 AT19671 AT19703 AT19704 AT21887 GH13220 GH23220 GM17319 SD01946 SD19406 SD22793
Other clones	EC15125 EC38617 EK128542 EK195518 EK217523 EK295109 EK300725 EK302855 EN00546 EP08213 FGH114H06 RP004006319 RP004083090 RP004103413 RP004117848 RP004118472 RP004139317 RP004148663 RP004194785 RP004207433 RP004209982 RP004212425 RP004237879 RP004241385 RP004253073 RP004265745 RP004315153 RP004318302 RP004347080 RP004364601 RP004400870 RP004427907 RP004466070 RP004480048 RP004480916 RP004481349 RP004485261 RP004512250 RP004516192 RP004518444 RP004526193 RP004530388 RP004530812 RP004546099 RP004546173
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0260639 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 33501
Antibody Information
	polyclonal (Wiese, 2008)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: γTub23C l(2)23Ce (Vázquez et al., 2008)
Additional comments	The "Pearl-2" allele of "γTub23C" (γTub23C[Pl-2]) maps to the same chromosomal location as the Pl[1] mutant allele of the "Pl, Pearl" locus. Both mutations have the same unique combination of mutant phenotypes, but the Pl[1] mutation is no longer extant and so the relationship between the "γTub23C" and "Pl" loci cannot be tested. (Vázquez et al., 2008)
Other Comments
	γTub23C is required for efficient recruitment of pericentriolar material. (Dobbelaere et al., 2008) RNAi screen using dsRNA made from templates generated with primers directed against this gene results in the formation of an aberrantly long, monopolar spindle when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA. (Goshima et al., 2007) SL2 cells treated with dsRNA against γTub23C show a clear increase in the mitotic index compared to control cells. The treated cells show bipolar monastral and monopolar anastral spindle organisation with metaphase-like chromosome arrangements. (Muller et al., 2006) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) Immunoblot analysis and immunolocalisation show that γTub23C and γTub37C are differentially expressed during gametogenesis and development. (Wilson et al., 1997) Subcellular localisation of the protein during the cell cycle of cells with and without centrioles is determined. Data suggests centrioles are not essential to the formation of a functional mitotic spindle, but participate in the focal concentration of γTub23C and γTub37C protein during both interphase and mitosis. (Debec et al., 1995) γTub23C is required for spermatogenesis. (Sampaio and Sunkel, 1995) γTub23C is required for the structure as well as the function of the microtubule organising center MTOC. (Sunkel et al., 1995) Drosophila γ tubulin is part of a complex containing Cp190 and Map60 gene products. (Raff et al., 1993) γTub23C has been cloned and sequenced. (Zheng et al., 1991) Isolated from a D.melanogaster ovary cDNA library using an A.nidulans mipA gene cDNA probe, under low stringency conditions. (Zheng and Oakley, 1989) A γTub23C cDNA has been cloned and sequenced. (Zheng and Oakley, 1989)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057456 RefSeq (Proteins) • NP_476804 DDBJ / EMBL / GenBank • AAA28597 AAC27620 AAF51174 AAL39778 AC010009 AC092187 AF073994 AI514354 AW943499 AY069633 M61765 Entrez Gene - A searchable database of RefSeq genes. • 33501 UniProtKB/Swiss-Prot • P23257
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Tubulin, conserved site (IPR017975) Tubulin/FtsZ, 2-layer sandwich domain (IPR018316) Tubulin (IPR000217) Gamma tubulin (IPR002454) Tubulin/FtsZ, GTPase domain (IPR003008) Tubulin/FtsZ, C-terminal (IPR008280) Tubulin, C-terminal (IPR023123) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD40196
Linkouts
	BioGRID - A database of protein and genetic interactions • 59721 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0260639 DroID - A comprehensive database of gene and protein interactions. • FBgn0260639 DRSC - Results from RNAi screens. • FBgn0260639 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0260639 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0260639 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 33501 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /cytoskel/tubgm1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 33501 modMine - Data generated by the modENCODE project. • FBgn0260639
Synonyms & Secondary IDs ( 46 )
Reported As
Symbol Synonym	CG3157 (Spellman and Rubin, 2002, Rebollo et al., 2004, Schubeler et al., 2002, Goldstein and Gunawardena, 2000, Goshima et al., 2007, Wirbelauer et al., 2005, Bell et al., 2007, Dobbelaere et al., 2008, Ducat et al., 2008, Keleman et al., 2009.8.5, Ni et al., 2008, Ni et al., 2010.12.1) CG 3157 (M Mahoney, 2006) D.m.GAMMA-23C (Goldstein and Gunawardena, 2000) GAMMA23C (Goldstein and Gunawardena, 2000) gammaTUB (Castellanos et al., 2008) gammaTub23C (Leemans et al., 2000, Giot et al., 2003, Bakal et al., 2007, Telonis-Scott et al., 2009, Japanese National Institute of Genetics, 2012.5.21) gammaTUB23C (Castellanos et al., 2008) gamma tubulin (Zhang and Megraw, 2006) GTU88* (Martinez-Campos et al., 2004, Basto et al., 2006) GTUB (Tsoi, 1998.6.19) g-Tubulin (Hutterer et al., 2003) l(2)23Ce (Vázquez et al., 2008, ) Tub23 (Spellman and Rubin, 2002) γzy (Wilson et al., 1992) γTub (Sato et al., 2011, Emery et al., 2005, Pai et al., 2004, Barros et al., 2005, Orian et al., 2007) γ-tub (Muller et al., 2006, Minakhina et al., 2007, Akbar et al., 2009) γtub (Prigent et al., 2005, Ghabrial et al., 1998, Harris and Peifer, 2004) γ-Tub (Pelissier et al., 2003, de Carcer et al., 2001, Hutterer et al., 2006, Anderson et al., 2009, Wang et al., 2011, Zhang and Megraw, 2007) γTUB (Herzig et al., 2002, Varmark et al., 2007) γ-tub23 (Vied and Horabin, 2001) γ-Tub23C (Rogers et al., 2008, Kai et al., 2005, Mahoney et al., 2006) γTub23C (Vázquez et al., 2008, Mahoney et al., 2006, Goshima et al., 2007, Wiese, 2008, Doheny et al., 2008, Dobbelaere et al., 2008, Rogers et al., 2008, Nguyen et al., 2011, Varmark, 2004, Whyard et al., 2009) γTUB23C (Tavosanis et al., 1997) γtub23C (Sampaio and Sunkel, 1995, Sunkel et al., 1995) γ-tub 23C (Giansanti et al., 2008) γ-tub23C (Raynaud-Messina et al., 2001) γTub23CD (Zheng et al., 1991, ) γ-tubulin (Bettencourt-Dias et al., 2004, Gunawardane et al., 2000) γ tubulin (Riparbelli et al., 2002)
Name Synonym	23C γ-tubulin (Colombie et al., 2006, Bouissou et al., 2009) 23C-γ-tubulin (Raynaud-Messina et al., 2004) &ggr-tubulin (Cheng et al., 2011, Nerusheva et al., 2009) gama-tubulin (Moutinho-Santos et al., 2001) gamma-tubulin (Kaltschmidt and Brand, 2002, Jeng and Stearns, 1999, Endow and Komma, 1998, Vied and Horabin, 1999, Rusan and Peifer, 2007, Martins et al., 2007) gamma tubulin (Megraw et al., 2000, Donaldson et al., 2001, Grieder et al., 2000) Gamma-tubulin (Baker and Kernan, 2001) lethal (2) 23Ce   tubulin (Goshima et al., 2007, Goshima et al., 2005, Ashley et al., 2005, Yuen et al., 2008) γ-tubulin (Dix and Raff, 2007, Chen et al., 2013, Salisbury, 2004, Januschke et al., 2005, Kwon and Scholey, 2004, Cottam et al., 2004, Greaves, 2001, Fuse et al., 2003, Albertson and Doe, 2003, Mathe et al., 2003, Gergely, 2002, Mollinari et al., 2002, Ohkura et al., 2001, Lee et al., 2001, Wakefield et al., 2001, Bolivar et al., 2001, MacDougall et al., 2001, Huynh et al., 2001, Ceron et al., 2001, Megraw et al., 2001, Gunawardane et al., 2000, Barbosa et al., 2000, Riparbelli et al., 2000, Grieder et al., 2000, Callaini et al., 1999, Su and Vidwans, 2000, Moritz et al., 2000, Lemos et al., 2000, Lange et al., 2000, Brent et al., 2000, Afshar et al., 2000, Sibon et al., 2000, Vidwans et al., 1999, Moutinho-Santos et al., 1999, Gunawardane et al., 1999, Bonaccorsi et al., 2000, Swan et al., 1999, Vaizel-Ohayon and Schejter, 1999, Gho et al., 1999, Megraw et al., 1999, Oegema et al., 1999, Bamburg et al., 1999, Herrmann et al., 1998, Lajoie-Mazenc et al., 1994, Marshall, 1998, Riparbelli and Callaini, 1998, Davis, 1997, Callaini et al., 1997, Li and Kaufman, 1996, Moritz et al., 1995, Moritz and Alberts, 1992, Wilson et al., 2004, Silvanovich et al., 2003, Gunawardane et al., 2003, Murphy, 2003, Prigent et al., 2005, Roper and Brown, 2004, Yamashita et al., 2003, Verollet et al., 2006, Siegrist and Doe, 2005, Wilson, 2005, Harris and Peifer, 2004, Yamashita et al., 2007, Yamashita et al., 2007, Ohlstein and Spradling, 2007, Barros et al., 2005, Caussinus and Gonzalez, 2005, Goshima et al., 2005, Rodrigues-Martins et al., 2007, Januschke et al., 2006, Morales-Mulia and Scholey, 2005, Mottier et al., 2008, Blagden and Glover, 2003, Zhang et al., 2007, Goshima et al., 2008, Tan et al., 2008, Wei et al., 2008, Trammell et al., 2008, Blachon et al., 2008, Colombié et al., 2008, Andersen and Tapon, 2008, Giansanti et al., 2008, Peel et al., 2007, Akbar et al., 2009, Mottier-Pavie and Megraw, 2009, Bouissou et al., 2009, Bucciarelli et al., 2009, Kracklauer et al., 2010, Mottier-Pavie et al., 2011, Yuan et al., 2012, Delgehyr et al., 2012, Sanghavi et al., 2012, Guerin and Kramer, 2009) γ tubulin (Fischer et al., 2004, Lee et al., 2001, Gunawardane et al., 2000, Schaefer et al., 2001, Donaldson et al., 2001, Foe et al., 2000, Zimmerman et al., 1999, Avides and Glover, 1999, Carmena et al., 1998, Moritz et al., 1998, Gigliotti et al., 1998, Bonaccorsi et al., 1998, Helps et al., 1998, Riparbelli et al., 1997, Stumpff et al., 2004, Goshima and Vale, 2003, Zheng and Oakley, 1989, Harris and Peifer, 2005) γ-Tubulin (Cha et al., 2002, Berdnik and Knoblich, 2002, Berdnik et al., 2001, Riparbelli et al., 2004, Megraw et al., 2008, Hutterer et al., 2006, Colombie et al., 2006, Martins et al., 2009, Rickmyre et al., 2007) γtubulin (Wilson, 2001, Kaltschmidt et al., 2000, Wilson, 1999, Wilson, 1999, Terada et al., 2003, Leatherman and Dinardo, 2010) γTubulin (Wang and Riechmann, 2008) γ-tubulin-1 (Debec et al., 1995) γ tubulin 23C (Somma et al., 2008) γ-tubulin 23C (Giansanti et al., 2008, Brodu et al., 2010) γ-tubulin (23C) (M Mahoney, 2006) γ-tubulin(23C) (Andersen and Tapon, 2008) γ-Tubulin at 23C (Ducat et al., 2008)
Secondary FlyBase IDs
	FBgn0004176 FBgn0040117
References ( 271 )
Generate a list of	All references relating to this gene ( 271 )
List References by type	Research paper  ( 182 ) Supplementary material  ( 14 ) Review  ( 20 ) Personal communication to FlyBase  ( 9 ) Abstract  ( 35 ) FlyBase analysis  ( 2 ) Conference report  ( 2 ) Letter  ( 1 ) DNA/RNA sequence record  ( 1 ) Stock list  ( 1 ) Protein sequence record  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 1 ) Book  ( 1 )
Recent research papers ( 11 )
	Chen et al., 2013, RNA 19(2): 208--218 BCAS2 is essential for Drosophila viability and functions in pre-mRNA splicing. [FBrf0220579] Chen et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109(29): 11842--11847 Axon injury and stress trigger a microtubule-based neuroprotective pathway. [FBrf0218884] Delgehyr et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109(15): 5729--5734 Drosophila Mgr, a Prefoldin subunit cooperating with von Hippel Lindau to regulate tubulin stability. [FBrf0218047] Fu and Glover, 2012, Open Biol. 2(8): 120104 Structured illumination of the interface between centriole and peri-centriolar material. [FBrf0219444] Sanghavi et al., 2012, Dev. Biol. 367(1): 66--77 A functional link between localized Oskar, dynamic microtubules, and endocytosis. [FBrf0218511] Yuan et al., 2012, Dev. Biol. 361(1): 57--67 Regulation of cyclin A localization downstream of Par-1 function is critical for the centrosome orientation checkpoint in Drosophila male germline stem cells. [FBrf0216861] Cheng et al., 2011, Development 138(5): 831--837 Asymmetric division of cyst stem cells in Drosophila testis is ensured by anaphase spindle repositioning. [FBrf0213009] Mottier-Pavie et al., 2011, J. Cell Sci. 124(5): 706--717 Phenotypic analysis of misato function reveals roles of noncentrosomal microtubules in Drosophila spindle formation. [FBrf0212996] Nguyen et al., 2011, Neural Dev. 6: 38 Microtubules are organized independently of the centrosome in Drosophila neurons. [FBrf0217384] Sato et al., 2011, Genes Dev. 25(22): 2361--2373 Maelstrom coordinates microtubule organization during Drosophila oogenesis through interaction with components of the MTOC. [FBrf0216748] Wang et al., 2011, Dev. Cell 21(3): 520--533 An ana2/ctp/mud complex regulates spindle orientation in Drosophila neuroblasts. [FBrf0215580] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...