FlyBase Gene Report: Dmel\mfas

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\mfas using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

mfas_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

expression microarray

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male

(Arbeitman et al., 2004)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

oogenesis stage S9 -- S10 & adult stage | female

follicle cell

(Arbeitman et al., 2004)

adult stage | female

female accessory gland

(Arbeitman et al., 2004)

fat body

(Arbeitman et al., 2004)

adult stage

central nervous system

(Arbeitman et al., 2004)

adult stage | male

testis

(Arbeitman et al., 2004)

male accessory gland

(Arbeitman et al., 2004)

seminal vesicle | restricted

Comment: surface where testis attaches

(Arbeitman et al., 2004)

ejaculatory bulb

(Arbeitman et al., 2004)

embryonic stage 4 -- 6

central brain anlage in statu nascendi

(Fisher et al., 2012)

dorsal ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

organism | segmentally repeated

(Fisher et al., 2012)

ventral ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

antennal anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

dorsal head epidermis anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

visual anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

embryonic stage 7 -- 8

dorsal ectoderm

(Fisher et al., 2012)

ventral ectoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

amnioserosa

(Fisher et al., 2012)

foregut primordium

(Fisher et al., 2012)

mesectoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

midline ventral glial cell

(Fisher et al., 2012)

tracheal primordium

(Fisher et al., 2012)

yolk nucleus

(Fisher et al., 2012)

embryonic stage 9 -- 12

midline primordium | subset

(Kearney et al., 2004)

embryonic stage 10 -- 11

embryonic/larval trachea

(Chung et al., 2011)

oogenesis

border follicle cell

(Wang et al., 2006)

centripetal follicle cell

(Wang et al., 2006)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male

(Arbeitman et al., 2004)

Additional Descriptive Data

mfas expression is enriched in border cells and in centripetal cells relative to other cells in the egg chamber.

(Wang et al., 2006)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Wasbrough et al., 2010)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

extracellular space

inferred from high throughput direct assay

(Findlay et al., 2008)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\mfas in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\mfas

View exonic expression by tissue for Dmel\mfas

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0260745

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0260745

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3359

Flygut - An atlas of the Drosophila adult midgut

FBgn0260745

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 5 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 8 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of mfas

Transgenic constructs containing regulatory region of mfas

Deletions and Duplications ( 6 )

Disrupted in

(Ryder, 2004.4.26)

(Ryder, 2004.4.26)

(Ryder, 2004.4.26)

(Hu et al., 1998)

(Hu et al., 1998)

Not disrupted in

Df(3R)ΔTk²

(Asahina et al., 2014)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^Mef2.PR

mfas^GD5174

viable

mfas^{MI11275-GFSTF.2}

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

mfas^GD5174

viable, with Scer\GAL4^pnr-MD237

viable, with Scer\GAL4^tin.CΔ4

mfas^GD5174

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (3)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\TGFBI

14 of 15

Yes

Hsap\POSTN

10 of 15

Yes

Hsap\STAB2

1 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (3)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Tgfbi

14 of 15

Yes

Mmus\Postn

9 of 15

Yes

Mmus\Stab2

1 of 15

Yes

Rattus norvegicus (Norway rat) (2)

Rnor\Tgfbi

13 of 13

Yes

Rnor\Postn

8 of 13

Yes

Xenopus tropicalis (Western clawed frog) (3)

Xtro\tgfbi

6 of 12

Yes

Xtro\postn

5 of 12

Xtro\LOC101733581

2 of 12

Yes

Danio rerio (Zebrafish) (4)

Drer\tgfbi

11 of 15

Yes

Drer\postna

9 of 15

Yes

Drer\postnb

9 of 15

Yes

Drer\stab2

1 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\F26E4.7

5 of 15

Yes

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\YLR001C

6 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\SPAC22H12.05c

5 of 12

Yes

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091902L3 )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA17404

Drosophila persimilis

Dper\GL21586

Drosophila willistoni

Dwil\GK11667

Drosophila virilis

Dvir\GJ23688

Drosophila mojavensis

Dmoj\GI24063

Drosophila grimshawi

Dgri\GH12578

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091500SH )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17010627

Musca domestica

House fly

Mdoa\17014429

Glossina morsitans

Tsetse fly

Gmor\GMOY001277

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_12951

Mayetiola destructor

Hessian fly

Mdes\Mdes007399

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL008657

Anopheles darlingi

American malaria mosquito

Adar\ADAC000708

Anopheles gambiae

Malaria mosquito

Agam\AGAP004426

Anopheles gambiae

Malaria mosquito

Agam\AGAP004428

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ006247

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W064M )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_11378

Tribolium castaneum

Red flour beetle

Tcas\TC012903

Rhodnius prolixus

Kissing bug

Rpro\RPRC001419

Cimex lectularius

Bed bug

Clec\CLEC012901

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_01878

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X017W )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR011743

Ixodes scapularis

Black-legged tick

Isca\ISCW001538

Stegodyphus mimosarum

African social velvet spider

Smim\KFM74623.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur21g00150

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_302377

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )

No non-Arthropod Metazoa orthologies identified

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (4)

Gene Symbol

Score

Source

Alignment

5 of 10

5 of 10

2 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 6 )

Human Ortholog

Disease

Evidence

References

TGFBI; transforming growth factor beta induced

model of lattice corneal dystrophy

IEA

(FlyBase, 2019-)

model of granular corneal dystrophy 1

IEA

(FlyBase, 2019-)

model of Thiel-Behnke corneal dystrophy

IEA

(FlyBase, 2019-)

model of epithelial basement membrane dystrophy

IEA

(FlyBase, 2019-)

model of granular corneal dystrophy 2

IEA

(FlyBase, 2019-)

model of Reis-Bucklers corneal dystrophy

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

mfas^GD5174

exacerbates hematologic cancer

modeled by hop^Tum

(Bina et al., 2010)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

TGFBI; transforming growth factor beta induced

14 of 15

601692

POSTN; periostin

10 of 15

608777

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

mfas - lms

two hybrid

(Hu et al., 2017.6.13)

mfas - osk

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Hurd et al., 2016)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

hop

enhanceable

mfas

(Bina et al., 2010)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

66572

DroID - A comprehensive database of gene and protein interactions.

FBgn0260745

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

41455

MIST (protein-protein) - An integrated Molecular Interaction Database

mfas

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-51

Cytogenetic map

87A7-87A7

Sequence location

3R:11,984,862..11,993,315 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

87A7-87A7

Left limit from molecular mapping relative to Hsp70Aa (FBrf0080055) Right limit from molecular mapping relative to Hsp70Ab (FBrf0080055)

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

87A7-87A9

(Hu, 1997.12.15, Glover et al., 1995)

87A4-87A9

(determined by in situ hybridisation)

(Hu et al., 1998)

Experimentally Determined Recombination Data

Location

3-51

(FlyBase, 2016)

3-49.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (8)

Bloomington

58256

y¹ v¹; P{TRiP.HMJ22320}attP40

56580

y¹ w^*; Mi{MIC}mfas^MI11275

63204

y¹ w^*; Mi{PT-GFSTF.2}mfas^{MI11275-GFSTF.2}

52905

y¹ sc^* v¹ sev²¹; P{TRiP.HMC03645}attP40

VDRC

v103621

P{KK101776}VIE-260B

v37888

w¹¹¹⁸; P{GD5174}v37888

v37889

w¹¹¹⁸; P{GD5174}v37889

v318000

PBac{fTRG00001.sfGFP-TVPTBF}VK00033

Genomic Clones (20)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (157)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0260745

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0260745

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

41455

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: mfas anon- EST:Liang-97

(Ashburner, 2004.6.30)

Source for merge of: mfas anon-WO0140519.174

(FlyBase, 1996-)

Source for merge of: mfas anon-87Af

(FlyBase, 1996-)

Additional comments

Source for merge of mfas anon-87Af was comparison of the chromosomal walk and transformation fragments shown in Figure 1. of FBrf0080055 with the corresponding region of the release 5.14 D.melanogaster genome.

(FlyBase, 1996-)

Source for merge of mfas anon-WO0140519.174 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Other Comments

Identified with: GH06752 <up>FlyBase curator comment: EST subsequently found to be chimeric</up>.

(Zou et al., 2000)

Mutants reveal weak defects in CNS axonogenesis. More severe defects are seen in double mutants with Abl.

(Hu et al., 1998)

EST GH06752 is chimeric; the 3' portion corresponds to part of mfas, the 5' portion matches part of AE003617 on the opposite strand to ninaC.

(FlyBase, 1996-)

Identified during molecular analysis of aur.

(Glover et al., 1995)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 87 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

41455

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0260745

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0260745

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

mfas_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3359

Flygut - An atlas of the Drosophila adult midgut

FBgn0260745

FlyMine - An integrated database for Drosophila genomics

FBgn0260745

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

41455

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC012903

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG3359

modMine - A data warehouse for the modENCODE project

FBgn0260745

Linkouts

BioGRID - A database of protein and genetic interactions.

66572

DroID - A comprehensive database of gene and protein interactions.

FBgn0260745

DRSC - Results frm RNAi screens

FBgn0260745

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0260745

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

41455

MIST (protein-protein) - An integrated Molecular Interaction Database

mfas

Synonyms and Secondary IDs (13)

Reported As

Symbol Synonym

87A7-9/7

(Glover et al., 1995)

BEST:GH06752

CG3359

(Çiçek et al., 2016, Hurd et al., 2016, Hosono et al., 2015, Coolon et al., 2012, Cammarato et al., 2011, Khanna et al., 2010, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Klebes et al., 2005, Mueller et al., 2005, Ashburner, 2004.6.30)

Mfas

(Betz and Darnell, 2006, Dierick and Greenspan, 2006)

anon-87Af

anon-EST:Liang-97

anon-WO0140519.174

clone 97

(Liang and Biggin, 1998)

gh06752

(Zou et al., 2000)

mfas

(Mu et al., 2019, Hu et al., 2017.6.13, Sarov et al., 2016, Aradska et al., 2015, Gene Disruption Project members, 2015-, Van Bortle et al., 2015, Asahina et al., 2014, Cernilogar et al., 2011, Chung et al., 2011, Bina et al., 2010, Wasbrough et al., 2010, Takemori and Yamamoto, 2009, Gilchrist et al., 2008, Jastrzebowska and Tomancak, 2007, Lecuyer et al., 2007, Muse et al., 2007, Ravi Ram and Wolfner, 2007, Jordan et al., 2005, Loop et al., 2004)

Name Synonyms

Midline Fasciclin

(Wang et al., 2006)

Midline fasciclin

(Khanna et al., 2010, Takemori and Yamamoto, 2009)

anon-87Af

midline fasciclin

(Cammarato et al., 2011, Jastrzebowska and Tomancak, 2007, Jordan et al., 2005)

Secondary FlyBase IDs

FBgn0024211
FBgn0025940
FBgn0043833
FBgn0044703
FBgn0015348

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (79)

The following external sites may use different assemblies or annotations than FlyBase.

Report Sections