l(2)k06323, MARK, l(2)27C1, dMARK, dPAR-1
Shares 5' UTR with upstream gene.
gene_with_stop_codon_read_through ; SO:0000697
Gene model reviewed during 5.44
Stop-codon suppression (UGA) postulated; FBrf0216884.
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Gene model reviewed during 5.48
Gene model includes transcripts encoding non-overlapping portions of the full CDS.
None of the polypeptides share 100% sequence identity.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\par-1 using the Feature Mapper tool.
par-1 protein is initially ubiquitously expressed in germline cells, but becomes localized to the presumptive oocyte after fusome disappearance, during oocyte specification. During oocyte polarization par-1 protein relocalizes from the anterior to the posterior of the developing oocyte. In stage 5 oocytes FBgn0260934:par-1 becomes tightly associated with the posterior cell cortex and does not overlap with FBgn0000163:baz @ protein.
par-1 protein localizes cortically within follicle cells at early stages of oogenesis. It is restricted to the basolateral membrane domain of the columnar epithelium in stage 10 egg chambers. The earliest germ line staining is in the fusome in the germarium. par-1 protein then localizes to the ring canals and the cortical cytoskeleton of the nurse cells. No asymmetric localization of par-1 protein is observed in the oocyte during stages 1-8 but in stage 9, a transient enrichment is observed in the anterior of the oocyte. par-1 protein then becomes progressively more concentrated at the posterior pole during stages 9 and 10.
GBrowse - Visual display of RNA-Seq signalsView Dmel\par-1 in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
One or more of the processed transcripts for this gene share(s) untranslated sequences with a transcript of an adjacent gene, but encode(s) a single open reading frame (ORF). The non-overlapping ORFs that share untranslated sequences are represented by par-1 and mei-W68.
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a greater than three-fold increase in AttA activity in response to heat-killed E.coli after ecdysone treatment in S2 cells.
When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an increase in the proportion of G2/M phase cells, an increase in cell size, a decrease in mitotic index and a whole range of mitotic abnormalities are seen.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R+ cells: reduced F-actin and altered cell shape. Kc167 cells show change from round to spindle-shaped, with the formation of F-actin puncta and microtubule extensions.
par-1 is required early in the germline for organisation of the microtubule cytoskeleton and subsequent oocyte determination. It has a second role late in oogenesis in axis determination.
Loss of par-1 function disrupts pole cell formation and abdominal patterning in the embryo. These defects arise from a disorganisation of the oocyte microtubule cytoskeleton.